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Conserved domains on  [gi|798548171|gb|AKA63287|]
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VP3 [Rotavirus I]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
capping_2-OMTase_viral super family cl41719
viral Cap-0 specific (nucleoside-2'-O-)-methyltransferase; Cap-0 specific (nucleoside-2'-O-) ...
 273-451 2.46e-05

viral Cap-0 specific (nucleoside-2'-O-)-methyltransferase; Cap-0 specific (nucleoside-2'-O-)-methyltransferase (2'OMTase) catalyzes the methylation of Cap-0 (m7GpppNp) at the 2'-hydroxyl of the ribose of the first nucleotide, using S-adenosyl-L-methionine (AdoMet) as the methyl donor. This reaction is the fourth and last step in mRNA capping, the creation of the stabilizing five-prime cap (5' cap) on mRNA. Some dsDNA and dsRNA viruses, like the bluetongue virus (BTV), a member of the Reoviridae family, and Vaccinia virus, a member of the Poxviridae family, as well as some ss(+)RNA viruses, like Flaviviridae and Nidovirales, cap their mRNAs and encode their own 2'OMTase. In BTV, all four reactions are catalyzed by a single protein, VP4. In Vaccinia, the activity is located in the processing factor of the poly(A) polymerase, VP39.


The actual alignment was detected with superfamily member cd20757:

Pssm-ID: 477759  Cd Length: 198  Bit Score: 45.89  E-value: 2.46e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 798548171 273 QNKNEHNFLKGLIAHCKKFGFPSRILYLGSYPSFWLENIPWFPAM-IHCYDPKyrqtNLKYVKWHTTE-FTVKDCSAIIS 350
Cdd:cd20757    2 QYKNMLNVLTTIYYYYNLYHKKPIIYMIGSAPSYWIYDVKQYSDFtFETWDPL----DTPYSTEHHKElFFIKDVIKLKD 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 798548171 351 NTYVYIDIRTDIRGMS--EDAFIREDNMIT--DIAMEISSKPscsvffKRKVLSDNFTFNDpIYPPDS---IHF-----G 418
Cdd:cd20757   78 NSILYIDIRTDRGNADwkEWRKLVEEETISnlEIAYKYLETG------KVKVCCVKMTAMD-IELPISaklLHHptteiR 150

                        170       180       190
                 ....*....|....*....|....*....|....*.
gi 798548171 419 KEYYNYM--SGVTN-KRKISKEDLFSGINSAITENI 451
Cdd:cd20757  151 SEFYLLLdkWDIKNiKRFIPKGVLYSFINNIITENV 186
 
Name Accession Description Interval E-value
capping_2-OMTase_Rotavirus cd20757
Cap-0 specific (nucleoside-2'-O-)-methyltransferase of rotavirus; Cap-0 specific (nucleoside-2 ...
 273-451 2.46e-05

Cap-0 specific (nucleoside-2'-O-)-methyltransferase of rotavirus; Cap-0 specific (nucleoside-2'-O-)-methyltransferase (2'OMTase) catalyzes the methylation of Cap-0 (m7GpppNp) at the 2'-hydroxyl of the ribose of the first nucleotide, using S-adenosyl-L-methionine (AdoMet) as the methyl donor. This reaction is the fourth and last step in mRNA capping, the creation of the stabilizing five-prime cap (5' cap) on mRNA. Rotaviruses, which comprise a family of dsRNA viruses, cap their mRNAs. The 2'OMTase activity is located in the multifunctional capping enzyme, VP3.


Pssm-ID: 467732  Cd Length: 198  Bit Score: 45.89  E-value: 2.46e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 798548171 273 QNKNEHNFLKGLIAHCKKFGFPSRILYLGSYPSFWLENIPWFPAM-IHCYDPKyrqtNLKYVKWHTTE-FTVKDCSAIIS 350
Cdd:cd20757    2 QYKNMLNVLTTIYYYYNLYHKKPIIYMIGSAPSYWIYDVKQYSDFtFETWDPL----DTPYSTEHHKElFFIKDVIKLKD 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 798548171 351 NTYVYIDIRTDIRGMS--EDAFIREDNMIT--DIAMEISSKPscsvffKRKVLSDNFTFNDpIYPPDS---IHF-----G 418
Cdd:cd20757   78 NSILYIDIRTDRGNADwkEWRKLVEEETISnlEIAYKYLETG------KVKVCCVKMTAMD-IELPISaklLHHptteiR 150

                        170       180       190
                 ....*....|....*....|....*....|....*.
gi 798548171 419 KEYYNYM--SGVTN-KRKISKEDLFSGINSAITENI 451
Cdd:cd20757  151 SEFYLLLdkWDIKNiKRFIPKGVLYSFINNIITENV 186
 
Name Accession Description Interval E-value
capping_2-OMTase_Rotavirus cd20757
Cap-0 specific (nucleoside-2'-O-)-methyltransferase of rotavirus; Cap-0 specific (nucleoside-2 ...
 273-451 2.46e-05

Cap-0 specific (nucleoside-2'-O-)-methyltransferase of rotavirus; Cap-0 specific (nucleoside-2'-O-)-methyltransferase (2'OMTase) catalyzes the methylation of Cap-0 (m7GpppNp) at the 2'-hydroxyl of the ribose of the first nucleotide, using S-adenosyl-L-methionine (AdoMet) as the methyl donor. This reaction is the fourth and last step in mRNA capping, the creation of the stabilizing five-prime cap (5' cap) on mRNA. Rotaviruses, which comprise a family of dsRNA viruses, cap their mRNAs. The 2'OMTase activity is located in the multifunctional capping enzyme, VP3.


Pssm-ID: 467732  Cd Length: 198  Bit Score: 45.89  E-value: 2.46e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 798548171 273 QNKNEHNFLKGLIAHCKKFGFPSRILYLGSYPSFWLENIPWFPAM-IHCYDPKyrqtNLKYVKWHTTE-FTVKDCSAIIS 350
Cdd:cd20757    2 QYKNMLNVLTTIYYYYNLYHKKPIIYMIGSAPSYWIYDVKQYSDFtFETWDPL----DTPYSTEHHKElFFIKDVIKLKD 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 798548171 351 NTYVYIDIRTDIRGMS--EDAFIREDNMIT--DIAMEISSKPscsvffKRKVLSDNFTFNDpIYPPDS---IHF-----G 418
Cdd:cd20757   78 NSILYIDIRTDRGNADwkEWRKLVEEETISnlEIAYKYLETG------KVKVCCVKMTAMD-IELPISaklLHHptteiR 150

                        170       180       190
                 ....*....|....*....|....*....|....*.
gi 798548171 419 KEYYNYM--SGVTN-KRKISKEDLFSGINSAITENI 451
Cdd:cd20757  151 SEFYLLLdkWDIKNiKRFIPKGVLYSFINNIITENV 186
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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