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Conserved domains on  [gi|770386604]
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Chain B, Aminoglycoside 3'-N-acetyltransferase

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 10456837)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  15581578|10940244|9175471|27367672|26818562
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 48-160 2.39e-17

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


:

Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 73.71  E-value: 2.39e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 770386604   48 KEFEDIPTYSDRQPTNEYLANLLHSETFIALAAFDRGTAIGGLAAYVLPKFEQarsEIYIYDLAVASSHRRLGVATALIS 127
Cdd:pfam00583   6 ELLSEEFPEPWPDEPLDLLEDWDEDASEGFFVAEEDGELVGFASLSIIDDEPP---VGEIEGLAVAPEYRGKGIGTALLQ 82

                          90       100       110
                  ....*....|....*....|....*....|...
gi 770386604  128 HLKRVAVELGAYVIYVQADYGDDPAVALYTKLG 160
Cdd:pfam00583  83 ALLEWARERGCERIFLEVAADNLAAIALYEKLG 115
 
Name Accession Description Interval E-value
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 48-160 2.39e-17

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 73.71  E-value: 2.39e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 770386604   48 KEFEDIPTYSDRQPTNEYLANLLHSETFIALAAFDRGTAIGGLAAYVLPKFEQarsEIYIYDLAVASSHRRLGVATALIS 127
Cdd:pfam00583   6 ELLSEEFPEPWPDEPLDLLEDWDEDASEGFFVAEEDGELVGFASLSIIDDEPP---VGEIEGLAVAPEYRGKGIGTALLQ 82

                          90       100       110
                  ....*....|....*....|....*....|...
gi 770386604  128 HLKRVAVELGAYVIYVQADYGDDPAVALYTKLG 160
Cdd:pfam00583  83 ALLEWARERGCERIFLEVAADNLAAIALYEKLG 115
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 92-173 1.57e-13

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 63.14  E-value: 1.57e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 770386604  92 AYVLPKFEQARSEIYIYDLAVASSHRRLGVATALISHLKRVAVELGAYVIYVQADYGDDPAVALYTKLG--VREDVMHFD 169
Cdd:COG0456    1 GFALLGLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGfeEVGERPNYY 80


                 ....
gi 770386604 170 IDPR 173
Cdd:COG0456   81 GDDA 84
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 78-143 9.90e-08

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 46.89  E-value: 9.90e-08
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 770386604  78 LAAFDRGTAIGGLAAYvlpKFEQARSEIYIYDLAVASSHRRLGVATALISHLKRVAVELGAYVIYV 143
Cdd:cd04301    2 LVAEDDGEIVGFASLS---PDGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRL 64
PRK03624 PRK03624
putative acetyltransferase; Provisional
 107-164 3.55e-07

putative acetyltransferase; Provisional


Pssm-ID: 235142 [Multi-domain]  Cd Length: 140  Bit Score: 47.23  E-value: 3.55e-07
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 770386604 107 IYDLAVASSHRRLGVATALISHLKRVAVELGAYVIYVQADYGDDPAVALYTKLGVRED 164
Cdd:PRK03624  71 AYYLAVHPDFRGRGIGRALVARLEKKLIARGCPKINLQVREDNDAVLGFYEALGYEEQ 128
 
Name Accession Description Interval E-value
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 48-160 2.39e-17

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 73.71  E-value: 2.39e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 770386604   48 KEFEDIPTYSDRQPTNEYLANLLHSETFIALAAFDRGTAIGGLAAYVLPKFEQarsEIYIYDLAVASSHRRLGVATALIS 127
Cdd:pfam00583   6 ELLSEEFPEPWPDEPLDLLEDWDEDASEGFFVAEEDGELVGFASLSIIDDEPP---VGEIEGLAVAPEYRGKGIGTALLQ 82

                          90       100       110
                  ....*....|....*....|....*....|...
gi 770386604  128 HLKRVAVELGAYVIYVQADYGDDPAVALYTKLG 160
Cdd:pfam00583  83 ALLEWARERGCERIFLEVAADNLAAIALYEKLG 115
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 92-173 1.57e-13

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 63.14  E-value: 1.57e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 770386604  92 AYVLPKFEQARSEIYIYDLAVASSHRRLGVATALISHLKRVAVELGAYVIYVQADYGDDPAVALYTKLG--VREDVMHFD 169
Cdd:COG0456    1 GFALLGLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGfeEVGERPNYY 80


                 ....
gi 770386604 170 IDPR 173
Cdd:COG0456   81 GDDA 84
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 51-160 2.00e-12

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 61.22  E-value: 2.00e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 770386604  51 EDIPTYSDRQPTNEYLAN---LLHSETFIAlaAFDRGTAIGGLAAYVLPKfeqarSEIYIYDLAVASSHRRLGVATALIS 127
Cdd:COG0454    9 EDINFILLIEALDAELKAmegSLAGAEFIA--VDDKGEPIGFAGLRRLDD-----KVLELKRLYVLPEYRGKGIGKALLE 81

                         90       100       110
                 ....*....|....*....|....*....|...
gi 770386604 128 HLKRVAVELGAYVIYVQADYGDDPAVALYTKLG 160
Cdd:COG0454   82 ALLEWARERGCTALELDTLDGNPAAIRFYERLG 114
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
33-160 6.65e-10

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 54.71  E-value: 6.65e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 770386604  33 PDEISAMRAVLDL-FGKEFEDiptysdrqptnEYLANLLHSETF-IALAAFDRGTAIGGLAAYVLPkFEQARSEIYIYDL 110
Cdd:COG3153    6 PEDAEAIAALLRAaFGPGREA-----------ELVDRLREDPAAgLSLVAEDDGEIVGHVALSPVD-IDGEGPALLLGPL 73

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 770386604 111 AVASSHRRLGVATALISHLKRVAVELGAYVIYVqadYGDDPAVALYTKLG 160
Cdd:COG3153   74 AVDPEYRGQGIGRALMRAALEAARERGARAVVL---LGDPSLLPFYERFG 120
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
55-160 2.56e-09

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 53.46  E-value: 2.56e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 770386604  55 TYSDRQPTNEYLANLL---HSETFIALAAFDRGTAIGgLAAYVLPKFEQARSEIYIYDLAVASSHRRLGVATALISHLKR 131
Cdd:COG1247   29 TFETEPPSEEEREAWFaaiLAPGRPVLVAEEDGEVVG-FASLGPFRPRPAYRGTAEESIYVDPDARGRGIGRALLEALIE 107

                         90       100
                 ....*....|....*....|....*....
gi 770386604 132 VAVELGAYVIYVQADYGDDPAVALYTKLG 160
Cdd:COG1247  108 RARARGYRRLVAVVLADNEASIALYEKLG 136
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 78-160 1.02e-08

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 50.15  E-value: 1.02e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 770386604   78 LAAFDRGTAIGGLAAYVLPkfeqARSEIYIYDLAVASSHRRLGVATALISHLKRVAVELGAYVIYVQAdygDDPAVALYT 157
Cdd:pfam13508   6 FVAEDDGKIVGFAALLPLD----DEGALAELRLAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLELET---TNRAAAFYE 78


                  ...
gi 770386604  158 KLG 160
Cdd:pfam13508  79 KLG 81
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
105-160 2.70e-08

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 49.14  E-value: 2.70e-08
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 770386604 105 IYIYDLAVASSHRRLGVATALISHLKRVAVELGAYVIYVQADYGDDPAVALYTKLG 160
Cdd:COG3393   16 AEISGVYTHPEYRGRGLASALVAALAREALARGARTPFLYVDADNPAARRLYERLG 71
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 78-143 9.90e-08

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 46.89  E-value: 9.90e-08
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 770386604  78 LAAFDRGTAIGGLAAYvlpKFEQARSEIYIYDLAVASSHRRLGVATALISHLKRVAVELGAYVIYV 143
Cdd:cd04301    2 LVAEDDGEIVGFASLS---PDGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRL 64
PRK03624 PRK03624
putative acetyltransferase; Provisional
 107-164 3.55e-07

putative acetyltransferase; Provisional


Pssm-ID: 235142 [Multi-domain]  Cd Length: 140  Bit Score: 47.23  E-value: 3.55e-07
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 770386604 107 IYDLAVASSHRRLGVATALISHLKRVAVELGAYVIYVQADYGDDPAVALYTKLGVRED 164
Cdd:PRK03624  71 AYYLAVHPDFRGRGIGRALVARLEKKLIARGCPKINLQVREDNDAVLGFYEALGYEEQ 128
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 80-160 7.05e-07

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 46.14  E-value: 7.05e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 770386604  80 AFDRGTAIGGLAAYVLPKfEQArseiYIYDLAVASSHRRLGVATALISHLKRVAVELGAYVIYVQAdygDDPAVALYTKL 159
Cdd:COG1246   33 AEEDGEIVGCAALHPLDE-DLA----ELRSLAVHPDYRGRGIGRRLLEALLAEARELGLKRLFLLT---TSAAIHFYEKL 104


                 .
gi 770386604 160 G 160
Cdd:COG1246  105 G 105
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 53-160 5.85e-06

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 43.80  E-value: 5.85e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 770386604   53 IPTYSD-------RQPTNEYLANLLHSETFIALAAFDrGTAIGGLAAyvlpkfeqARSEIYIYDLAVASSHRRLGVATAL 125
Cdd:pfam13673   2 APDYSEegietfyEFISPEALRERIDQGEYFFFVAFE-GGQIVGVIA--------LRDRGHISLLFVDPDYQGQGIGKAL 72

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 770386604  126 ISHLKRVAVELGAYVIYVQADyGDDPAVALYTKLG 160
Cdd:pfam13673  73 LEAVEDYAEKDGIKLSELTVN-ASPYAVPFYEKLG 106
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
110-160 1.19e-04

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 40.17  E-value: 1.19e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 770386604 110 LAVASSHRRLGVATALISHLKRVAVELGAYVIYVQADYgddPAVALYTKLG 160
Cdd:COG2153   64 VAVLPEYRGQGLGRALMEAAIEEARERGARRIVLSAQA---HAVGFYEKLG 111
Eis COG4552
Predicted acetyltransferase [General function prediction only];
30-140 4.24e-04

Predicted acetyltransferase [General function prediction only];


Pssm-ID: 443616 [Multi-domain]  Cd Length: 393  Bit Score: 39.88  E-value: 4.24e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 770386604  30 KIGPDEISAMRAVLDL-FGKEFEDiptysdrQPTNEYLANLLHSETFialAAFDRGTAIGGLAAYvlpKFEQA-RSEIY- 106
Cdd:COG4552    5 PLTEDDLDAFARLLAYaFGPEPDD-------EELEAYRPLLEPGRVL---GVFDDGELVGTLALY---PFTLNvGGARVp 71

                         90       100       110
                 ....*....|....*....|....*....|....*..
gi 770386604 107 ---IYDLAVASSHRRLGVATALISHLKRVAVELGAYV 140
Cdd:COG4552   72 magITGVAVAPEHRRRGVARALLREALAELRERGQPL 108
PTZ00330 PTZ00330
acetyltransferase; Provisional
 87-170 1.42e-03

acetyltransferase; Provisional


Pssm-ID: 140351 [Multi-domain]  Cd Length: 147  Bit Score: 37.51  E-value: 1.42e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 770386604  87 IGGLAAYVLPKFEQA-RSEIYIYDLAVASSHRRLGVATALISHLKRVAVELGAYVIYVqaDYGDDpAVALYTKLGVR--E 163
Cdd:PTZ00330  64 VGTASLFVEPKFTRGgKCVGHIEDVVVDPSYRGQGLGRALISDLCEIARSSGCYKVIL--DCTED-MVAFYKKLGFRacE 140


                 ....*..
gi 770386604 164 DVMHFDI 170
Cdd:PTZ00330 141 RQMRLDL 147
FR47 pfam08445
FR47-like protein; The members of this family are similar to the C-terminal region of the D. ...
 106-164 3.74e-03

FR47-like protein; The members of this family are similar to the C-terminal region of the D. melanogaster hypothetical protein FR47. This protein has been found to consist of two N-acyltransferase-like domains swapped with the C-terminal strands.


Pssm-ID: 117022 [Multi-domain]  Cd Length: 86  Bit Score: 35.00  E-value: 3.74e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 770386604  106 YIYDLAVASSHRRLGVATALISHLKRVAVELGaYVIYVQADYGDDPAVALYTKLGVRED 164
Cdd:pfam08445  23 ELGALQTLPEHRRRGLGSRLVAALARGIAERG-ITPFAVVVAGNTPSRRLYEKLGFRKI 80
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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