|
Name |
Accession |
Description |
Interval |
E-value |
| SEC23 |
COG5047 |
Vesicle coat complex COPII, subunit SEC23 [Intracellular trafficking and secretion]; |
1-767 |
0e+00 |
|
Vesicle coat complex COPII, subunit SEC23 [Intracellular trafficking and secretion];
Pssm-ID: 227380 [Multi-domain] Cd Length: 755 Bit Score: 1266.73 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 1 MDFETNEDINGVRFTWNVFPSTRSDANSNVVPVGCLYTPLKEYDELNVAPYNPVVCSGPhCKSILNPYCVIDPRNSSWSC 80
Cdd:COG5047 1 MNFEIIEENDGIRLTWNVFPATRGDATRTVIPIACLYTPLHEDDALTVNYYEPVKCTAP-CKAVLNPYCHIDERNQSWIC 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 81 PICNSRNHLPPQYTNLSQENMPLEL--QSTTIEYITNKPVTVPPIFFFVVDLTSETENLDSLKESIITSLSLLPPNALIG 158
Cdd:COG5047 80 PFCNQRNTLPPQYRDISNANLPLELlpQSSTIEYTLSKPVILPPVFFFVVDACCDEEELTALKDSLIVSLSLLPPEALVG 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 159 LITYGNVVQLHDLSSETIDRCNVFRGDREYQLEALTEMLTGQKPTGPGGAASHLPNAMNkvtpFSLNRFFLPLEQVEFKL 238
Cdd:COG5047 160 LITYGTSIQVHELNAENHRRSYVFSGNKEYTKENLQELLALSKPTKSGGFESKISGIGQ----FASSRFLLPTQQCEFKL 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 239 NQLLENLSPDQWSVPAGHRPLRATGSALNIASLLLQGCYKNIPARIILFASGPGTVAPGLIVNSELKDPLRSHHDIDSDH 318
Cdd:COG5047 236 LNILEQLQPDPWPVPAGKRPLRCTGSALNIASSLLEQCFPNAGCHIVLFAGGPCTVGPGTVVSTELKEPMRSHHDIESDS 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 319 AQHYKKACKFYNQIAQRVAANGHTVDIFAGCYDQIGMSEMKQLTDSTGGVLLLTDAFSTAIFKQSYLRLFAKDEEGYLKM 398
Cdd:COG5047 316 AQHSKKATKFYKGLAERVANQGHALDIFAGCLDQIGIMEMEPLTTSTGGALVLSDSFTTSIFKQSFQRIFNRDSEGYLKM 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 399 AFNGNMAVKTSKDLKVQGLIGHASAVKKtDANNISESEIGIGATSTWKMASLSPYHSYAIFFEIANTAANSNPmmsapgs 478
Cdd:COG5047 396 GFNANMEVKTSKNLKIKGLIGHAVSVKK-KANNISDSEIGIGATNSWKMASLSPKSNYALYFEIALGAASGSA------- 467
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 479 aDRPHLAYTQFITTYQHSSGTNRIRVTTVANQLLPFGTPAIAASFDQEAAAVLMARIAVHKAETDDGADVIRWLDRTLIK 558
Cdd:COG5047 468 -QRPAEAYIQFITTYQHSSGTYRIRVTTVARMFTDGGLPKINRSFDQEAAAVFMARIAAFKAETEDIIDVFRWIDRNLIR 546
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 559 LCQKYADYNKDDPQSFRLAPNFSLYPQFTYYLRRSQFLSVFNNSPDETAFYRHIFTREDTTNSLIMIQPTLTSFSMEDDP 638
Cdd:COG5047 547 LCQKFADYRKDDPSSFRLDPNFTLYPQFMYHLRRSPFLSVFNNSPDETAFYRHMLNNADVNDSLIMIQPTLQSYSFEKGG 626
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 639 QPVLLDSISVKPNTILLLDTFFFILIYHGEQIAQWRKAGYQDDPQYADFKALLEEPKLEAAELLVDRFPLPRFIDTEAGG 718
Cdd:COG5047 627 VPVLLDSVSVKPDVILLLDTFFHILIFHGSYIAQWRNAGYQEQPEYLNLKELLEAPRLEAAELLQDRFPIPRFIVTEQGG 706
|
730 740 750 760
....*....|....*....|....*....|....*....|....*....
gi 768899196 719 SQARFLLSKLNPSDNYQDMARGGSTIVLTDDVSLQNFMTHLQQVAVSGQ 767
Cdd:COG5047 707 SQARFLLSKINPSDITNKMSGGGSETILTDDVNLQKFMNHLRKLAVSKS 755
|
|
| PLN00162 |
PLN00162 |
transport protein sec23; Provisional |
1-766 |
0e+00 |
|
transport protein sec23; Provisional
Pssm-ID: 215083 [Multi-domain] Cd Length: 761 Bit Score: 1005.62 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 1 MDFETNEDINGVRFTWNVFPSTRSDANSNVVPVGCLYTPLKEYDELNVAPYNPVVCSGphCKSILNPYCVIDPRNSSWSC 80
Cdd:PLN00162 1 MDFAELEAIDGVRMSWNVWPSSKIEASKCVIPLAALYTPLKPLPELPVLPYDPLRCRT--CRAVLNPYCRVDFQAKIWIC 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 81 PICNSRNHLPPQYTNLSQENMPLEL--QSTTIEYITNKPVTV---PPIFFFVVDLTSETENLDSLKESIITSLSLLPPNA 155
Cdd:PLN00162 79 PFCFQRNHFPPHYSSISETNLPAELfpQYTTVEYTLPPGSGGapsPPVFVFVVDTCMIEEELGALKSALLQAIALLPENA 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 156 LIGLITYGNVVQLHDLSSETIDRCNVFRGDREYQLEALTEML----TGQKPTGPGGAashlpNAMNKVTPFSLNRFFLPL 231
Cdd:PLN00162 159 LVGLITFGTHVHVHELGFSECSKSYVFRGNKEVSKDQILEQLglggKKRRPAGGGIA-----GARDGLSSSGVNRFLLPA 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 232 EQVEFKLNQLLENLSPDQWSVPAGHRPLRATGSALNIASLLLQGCYKNIPARIILFASGPGTVAPGLIVNSELKDPLRSH 311
Cdd:PLN00162 234 SECEFTLNSALEELQKDPWPVPPGHRPARCTGAALSVAAGLLGACVPGTGARIMAFVGGPCTEGPGAIVSKDLSEPIRSH 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 312 HDIDSDHAQHYKKACKFYNQIAQRVAANGHTVDIFAGCYDQIGMSEMKQLTDSTGGVLLLTDAFSTAIFKQSYLRLFAKD 391
Cdd:PLN00162 314 KDLDKDAAPYYKKAVKFYEGLAKQLVAQGHVLDVFACSLDQVGVAEMKVAVERTGGLVVLAESFGHSVFKDSLRRVFERD 393
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 392 EEGYLKMAFNGNMAVKTSKDLKVQGLIGHASAVKKTdANNISESEIGIGATSTWKMASLSPYHSYAIFFEIANTAANSNP 471
Cdd:PLN00162 394 GEGSLGLSFNGTFEVNCSKDVKVQGAIGPCASLEKK-GPSVSDTEIGEGGTTAWKLCGLDKKTSLAVFFEVANSGQSNPQ 472
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 472 mmsAPGSAdrphlAYTQFITTYQHSSGTNRIRVTTVANQLL-PFGTPAIAASFDQEAAAVLMARIAVHKAETDDGADVIR 550
Cdd:PLN00162 473 ---PPGQQ-----FFLQFLTRYQHSNGQTRLRVTTVTRRWVeGSSSEELVAGFDQEAAAVVMARLASHKMETEEEFDATR 544
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 551 WLDRTLIKLCQKYADYNKDDPQSFRLAPNFSLYPQFTYYLRRSQFLSVFNNSPDETAFYRHIFTREDTTNSLIMIQPTLT 630
Cdd:PLN00162 545 WLDRALIRLCSKFGDYRKDDPSSFRLSPNFSLYPQFMFNLRRSQFVQVFNNSPDETAYFRMMLNRENVTNSLVMIQPTLI 624
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 631 SFSMEDDPQPVLLDSISVKPNTILLLDTFFFILIYHGEQIAQWRKAGYQDDPQYADFKALLEEPKLEAAELLVDRFPLPR 710
Cdd:PLN00162 625 SYSFNGPPEPVLLDVASIAADRILLLDSYFSVVIFHGSTIAQWRKAGYHNQPEHEAFAQLLEAPQADAQAIIKERFPVPR 704
|
730 740 750 760 770
....*....|....*....|....*....|....*....|....*....|....*..
gi 768899196 711 FIDTEAGGSQARFLLSKLNPSDNYQ-DMARGGSTIVLTDDVSLQNFMTHLQQVAVSG 766
Cdd:PLN00162 705 LVVCDQHGSQARFLLAKLNPSATYNsANAMGGSDIIFTDDVSLQVFMEHLQRLAVQS 761
|
|
| Sec23-like |
cd01478 |
Sec23-like: Protein and membrane traffic in eukaryotes is mediated by at least in part by the ... |
119-386 |
3.67e-162 |
|
Sec23-like: Protein and membrane traffic in eukaryotes is mediated by at least in part by the budding and fusion of intracellular transport vesicles that selectively carry cargo proteins and lipids from donor to acceptor organelles. The two main classes of vesicular carriers within the endocytic and the biosynthetic pathways are COP- and clathrin-coated vesicles. Formation of COPII vesicles requires the ordered assembly of the coat built from several cytosolic components GTPase Sar1, complexes of Sec23-Sec24 and Sec13-Sec31. The process is initiated by the conversion of GDP to GTP by the GTPase Sar1 which then recruits the heterodimeric complex of Sec23 and Sec24. This heterodimeric complex generates the pre-budding complex. The final step leading to membrane deformation and budding of COPII-coated vesicles is carried by the heterodimeric complex Sec13-Sec31. The members of this CD belong to the Sec23-like family. Sec 23 is very similar to Sec24. The Sec23 and Sec24 polypeptides fold into five distinct domains: a beta-barrel, a zinc finger, a vWA or trunk, an all helical region and a carboxy Gelsolin domain. The members of this subgroup lack the consensus MIDAS motif but have the overall Para-Rossmann type fold that is characteristic of this superfamily.
Pssm-ID: 238755 [Multi-domain] Cd Length: 267 Bit Score: 469.54 E-value: 3.67e-162
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 119 TVPPIFFFVVDLTSETENLDSLKESIITSLSLLPPNALIGLITYGNVVQLHDLSSETIDRCNVFRGDREYQLEALTEMLT 198
Cdd:cd01478 1 TSPPVFLFVVDTCMDEEELDALKESLIMSLSLLPPNALVGLITFGTMVQVHELGFEECSKSYVFRGNKDYTAKQIQDMLG 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 199 GQKPTGPGGAASHlPNAMNKVTPFSLNRFFLPLEQVEFKLNQLLENLSPDQWSVPAGHRPLRATGSALNIASLLLQGCYK 278
Cdd:cd01478 81 LGGPAMRPSASQH-PGAGNPLPSAAASRFLLPVSQCEFTLTDLLEQLQPDPWPVPAGHRPLRCTGVALSIAVGLLEACFP 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 279 NIPARIILFASGPGTVAPGLIVNSELKDPLRSHHDIDSDHAQHYKKACKFYNQIAQRVAANGHTVDIFAGCYDQIGMSEM 358
Cdd:cd01478 160 NTGARIMLFAGGPCTVGPGAVVSTELKDPIRSHHDIDKDNAKYYKKAVKFYDSLAKRLAANGHAVDIFAGCLDQVGLLEM 239
|
250 260
....*....|....*....|....*...
gi 768899196 359 KQLTDSTGGVLLLTDAFSTAIFKQSYLR 386
Cdd:cd01478 240 KVLVNSTGGHVVLSDSFTTSIFKQSFQR 267
|
|
| Sec23_trunk |
pfam04811 |
Sec23/Sec24 trunk domain; COPII-coated vesicles carry proteins from the endoplasmic reticulum ... |
121-388 |
3.47e-83 |
|
Sec23/Sec24 trunk domain; COPII-coated vesicles carry proteins from the endoplasmic reticulum to the Golgi complex. This vesicular transport can be reconstituted by using three cytosolic components containing five proteins: the small GTPase Sar1p, the Sec23p/24p complex, and the Sec13p/Sec31p complex. This domain is known as the trunk domain and has an alpha/beta vWA fold and forms the dimer interface.
Pssm-ID: 398467 [Multi-domain] Cd Length: 241 Bit Score: 264.50 E-value: 3.47e-83
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 121 PPIFFFVVDLT---SETENLDSLKESIITSLSLLP--PNALIGLITYGNVVQLHDLSSETidrcnvfRGDREYQLEALTE 195
Cdd:pfam04811 3 PPVFLFVIDVSynaIKSGLLAALKESLLQSLDLLPgdPRARVGFITFDSTVHFFNLGSSL-------RQPQMLVVSDLQD 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 196 MLTgqkptgpggaashlpnamnkvtPFSlNRFFLPLEQVEFKLNQLLENLsPDQWSVPagHRPLRATGSALNIASLLLQG 275
Cdd:pfam04811 76 MFL----------------------PLP-DRFLVPLSECRFVLEDLLEQL-PPMFPVT--KRPERCLGPALQAAFLLLKA 129
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 276 CYKniPARIILFASGPGTVAPGLIVNSELKDplrSHHDIDSDHAQHYKKACKFYNQIAQRVAANGHTVDIFAGCYDQIGM 355
Cdd:pfam04811 130 AFT--GGKIMVFQGGLPTVGPGGKLKSRLDE---SHHGTDKEKAKLVKKADKFYKSLAKECVKQGHSVDLFAFSLDYVDV 204
|
250 260 270
....*....|....*....|....*....|....*..
gi 768899196 356 SEMKQLTDSTGGVLLLTDAFS----TAIFKQSYLRLF 388
Cdd:pfam04811 205 ATLGQLSRLTGGQVYLYPSFQadvdGSKFKQDLQRYF 241
|
|
| |
|
Name |
Accession |
Description |
Interval |
E-value |
| SEC23 |
COG5047 |
Vesicle coat complex COPII, subunit SEC23 [Intracellular trafficking and secretion]; |
1-767 |
0e+00 |
|
Vesicle coat complex COPII, subunit SEC23 [Intracellular trafficking and secretion];
Pssm-ID: 227380 [Multi-domain] Cd Length: 755 Bit Score: 1266.73 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 1 MDFETNEDINGVRFTWNVFPSTRSDANSNVVPVGCLYTPLKEYDELNVAPYNPVVCSGPhCKSILNPYCVIDPRNSSWSC 80
Cdd:COG5047 1 MNFEIIEENDGIRLTWNVFPATRGDATRTVIPIACLYTPLHEDDALTVNYYEPVKCTAP-CKAVLNPYCHIDERNQSWIC 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 81 PICNSRNHLPPQYTNLSQENMPLEL--QSTTIEYITNKPVTVPPIFFFVVDLTSETENLDSLKESIITSLSLLPPNALIG 158
Cdd:COG5047 80 PFCNQRNTLPPQYRDISNANLPLELlpQSSTIEYTLSKPVILPPVFFFVVDACCDEEELTALKDSLIVSLSLLPPEALVG 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 159 LITYGNVVQLHDLSSETIDRCNVFRGDREYQLEALTEMLTGQKPTGPGGAASHLPNAMNkvtpFSLNRFFLPLEQVEFKL 238
Cdd:COG5047 160 LITYGTSIQVHELNAENHRRSYVFSGNKEYTKENLQELLALSKPTKSGGFESKISGIGQ----FASSRFLLPTQQCEFKL 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 239 NQLLENLSPDQWSVPAGHRPLRATGSALNIASLLLQGCYKNIPARIILFASGPGTVAPGLIVNSELKDPLRSHHDIDSDH 318
Cdd:COG5047 236 LNILEQLQPDPWPVPAGKRPLRCTGSALNIASSLLEQCFPNAGCHIVLFAGGPCTVGPGTVVSTELKEPMRSHHDIESDS 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 319 AQHYKKACKFYNQIAQRVAANGHTVDIFAGCYDQIGMSEMKQLTDSTGGVLLLTDAFSTAIFKQSYLRLFAKDEEGYLKM 398
Cdd:COG5047 316 AQHSKKATKFYKGLAERVANQGHALDIFAGCLDQIGIMEMEPLTTSTGGALVLSDSFTTSIFKQSFQRIFNRDSEGYLKM 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 399 AFNGNMAVKTSKDLKVQGLIGHASAVKKtDANNISESEIGIGATSTWKMASLSPYHSYAIFFEIANTAANSNPmmsapgs 478
Cdd:COG5047 396 GFNANMEVKTSKNLKIKGLIGHAVSVKK-KANNISDSEIGIGATNSWKMASLSPKSNYALYFEIALGAASGSA------- 467
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 479 aDRPHLAYTQFITTYQHSSGTNRIRVTTVANQLLPFGTPAIAASFDQEAAAVLMARIAVHKAETDDGADVIRWLDRTLIK 558
Cdd:COG5047 468 -QRPAEAYIQFITTYQHSSGTYRIRVTTVARMFTDGGLPKINRSFDQEAAAVFMARIAAFKAETEDIIDVFRWIDRNLIR 546
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 559 LCQKYADYNKDDPQSFRLAPNFSLYPQFTYYLRRSQFLSVFNNSPDETAFYRHIFTREDTTNSLIMIQPTLTSFSMEDDP 638
Cdd:COG5047 547 LCQKFADYRKDDPSSFRLDPNFTLYPQFMYHLRRSPFLSVFNNSPDETAFYRHMLNNADVNDSLIMIQPTLQSYSFEKGG 626
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 639 QPVLLDSISVKPNTILLLDTFFFILIYHGEQIAQWRKAGYQDDPQYADFKALLEEPKLEAAELLVDRFPLPRFIDTEAGG 718
Cdd:COG5047 627 VPVLLDSVSVKPDVILLLDTFFHILIFHGSYIAQWRNAGYQEQPEYLNLKELLEAPRLEAAELLQDRFPIPRFIVTEQGG 706
|
730 740 750 760
....*....|....*....|....*....|....*....|....*....
gi 768899196 719 SQARFLLSKLNPSDNYQDMARGGSTIVLTDDVSLQNFMTHLQQVAVSGQ 767
Cdd:COG5047 707 SQARFLLSKINPSDITNKMSGGGSETILTDDVNLQKFMNHLRKLAVSKS 755
|
|
| PLN00162 |
PLN00162 |
transport protein sec23; Provisional |
1-766 |
0e+00 |
|
transport protein sec23; Provisional
Pssm-ID: 215083 [Multi-domain] Cd Length: 761 Bit Score: 1005.62 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 1 MDFETNEDINGVRFTWNVFPSTRSDANSNVVPVGCLYTPLKEYDELNVAPYNPVVCSGphCKSILNPYCVIDPRNSSWSC 80
Cdd:PLN00162 1 MDFAELEAIDGVRMSWNVWPSSKIEASKCVIPLAALYTPLKPLPELPVLPYDPLRCRT--CRAVLNPYCRVDFQAKIWIC 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 81 PICNSRNHLPPQYTNLSQENMPLEL--QSTTIEYITNKPVTV---PPIFFFVVDLTSETENLDSLKESIITSLSLLPPNA 155
Cdd:PLN00162 79 PFCFQRNHFPPHYSSISETNLPAELfpQYTTVEYTLPPGSGGapsPPVFVFVVDTCMIEEELGALKSALLQAIALLPENA 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 156 LIGLITYGNVVQLHDLSSETIDRCNVFRGDREYQLEALTEML----TGQKPTGPGGAashlpNAMNKVTPFSLNRFFLPL 231
Cdd:PLN00162 159 LVGLITFGTHVHVHELGFSECSKSYVFRGNKEVSKDQILEQLglggKKRRPAGGGIA-----GARDGLSSSGVNRFLLPA 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 232 EQVEFKLNQLLENLSPDQWSVPAGHRPLRATGSALNIASLLLQGCYKNIPARIILFASGPGTVAPGLIVNSELKDPLRSH 311
Cdd:PLN00162 234 SECEFTLNSALEELQKDPWPVPPGHRPARCTGAALSVAAGLLGACVPGTGARIMAFVGGPCTEGPGAIVSKDLSEPIRSH 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 312 HDIDSDHAQHYKKACKFYNQIAQRVAANGHTVDIFAGCYDQIGMSEMKQLTDSTGGVLLLTDAFSTAIFKQSYLRLFAKD 391
Cdd:PLN00162 314 KDLDKDAAPYYKKAVKFYEGLAKQLVAQGHVLDVFACSLDQVGVAEMKVAVERTGGLVVLAESFGHSVFKDSLRRVFERD 393
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 392 EEGYLKMAFNGNMAVKTSKDLKVQGLIGHASAVKKTdANNISESEIGIGATSTWKMASLSPYHSYAIFFEIANTAANSNP 471
Cdd:PLN00162 394 GEGSLGLSFNGTFEVNCSKDVKVQGAIGPCASLEKK-GPSVSDTEIGEGGTTAWKLCGLDKKTSLAVFFEVANSGQSNPQ 472
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 472 mmsAPGSAdrphlAYTQFITTYQHSSGTNRIRVTTVANQLL-PFGTPAIAASFDQEAAAVLMARIAVHKAETDDGADVIR 550
Cdd:PLN00162 473 ---PPGQQ-----FFLQFLTRYQHSNGQTRLRVTTVTRRWVeGSSSEELVAGFDQEAAAVVMARLASHKMETEEEFDATR 544
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 551 WLDRTLIKLCQKYADYNKDDPQSFRLAPNFSLYPQFTYYLRRSQFLSVFNNSPDETAFYRHIFTREDTTNSLIMIQPTLT 630
Cdd:PLN00162 545 WLDRALIRLCSKFGDYRKDDPSSFRLSPNFSLYPQFMFNLRRSQFVQVFNNSPDETAYFRMMLNRENVTNSLVMIQPTLI 624
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 631 SFSMEDDPQPVLLDSISVKPNTILLLDTFFFILIYHGEQIAQWRKAGYQDDPQYADFKALLEEPKLEAAELLVDRFPLPR 710
Cdd:PLN00162 625 SYSFNGPPEPVLLDVASIAADRILLLDSYFSVVIFHGSTIAQWRKAGYHNQPEHEAFAQLLEAPQADAQAIIKERFPVPR 704
|
730 740 750 760 770
....*....|....*....|....*....|....*....|....*....|....*..
gi 768899196 711 FIDTEAGGSQARFLLSKLNPSDNYQ-DMARGGSTIVLTDDVSLQNFMTHLQQVAVSG 766
Cdd:PLN00162 705 LVVCDQHGSQARFLLAKLNPSATYNsANAMGGSDIIFTDDVSLQVFMEHLQRLAVQS 761
|
|
| Sec23-like |
cd01478 |
Sec23-like: Protein and membrane traffic in eukaryotes is mediated by at least in part by the ... |
119-386 |
3.67e-162 |
|
Sec23-like: Protein and membrane traffic in eukaryotes is mediated by at least in part by the budding and fusion of intracellular transport vesicles that selectively carry cargo proteins and lipids from donor to acceptor organelles. The two main classes of vesicular carriers within the endocytic and the biosynthetic pathways are COP- and clathrin-coated vesicles. Formation of COPII vesicles requires the ordered assembly of the coat built from several cytosolic components GTPase Sar1, complexes of Sec23-Sec24 and Sec13-Sec31. The process is initiated by the conversion of GDP to GTP by the GTPase Sar1 which then recruits the heterodimeric complex of Sec23 and Sec24. This heterodimeric complex generates the pre-budding complex. The final step leading to membrane deformation and budding of COPII-coated vesicles is carried by the heterodimeric complex Sec13-Sec31. The members of this CD belong to the Sec23-like family. Sec 23 is very similar to Sec24. The Sec23 and Sec24 polypeptides fold into five distinct domains: a beta-barrel, a zinc finger, a vWA or trunk, an all helical region and a carboxy Gelsolin domain. The members of this subgroup lack the consensus MIDAS motif but have the overall Para-Rossmann type fold that is characteristic of this superfamily.
Pssm-ID: 238755 [Multi-domain] Cd Length: 267 Bit Score: 469.54 E-value: 3.67e-162
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 119 TVPPIFFFVVDLTSETENLDSLKESIITSLSLLPPNALIGLITYGNVVQLHDLSSETIDRCNVFRGDREYQLEALTEMLT 198
Cdd:cd01478 1 TSPPVFLFVVDTCMDEEELDALKESLIMSLSLLPPNALVGLITFGTMVQVHELGFEECSKSYVFRGNKDYTAKQIQDMLG 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 199 GQKPTGPGGAASHlPNAMNKVTPFSLNRFFLPLEQVEFKLNQLLENLSPDQWSVPAGHRPLRATGSALNIASLLLQGCYK 278
Cdd:cd01478 81 LGGPAMRPSASQH-PGAGNPLPSAAASRFLLPVSQCEFTLTDLLEQLQPDPWPVPAGHRPLRCTGVALSIAVGLLEACFP 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 279 NIPARIILFASGPGTVAPGLIVNSELKDPLRSHHDIDSDHAQHYKKACKFYNQIAQRVAANGHTVDIFAGCYDQIGMSEM 358
Cdd:cd01478 160 NTGARIMLFAGGPCTVGPGAVVSTELKDPIRSHHDIDKDNAKYYKKAVKFYDSLAKRLAANGHAVDIFAGCLDQVGLLEM 239
|
250 260
....*....|....*....|....*...
gi 768899196 359 KQLTDSTGGVLLLTDAFSTAIFKQSYLR 386
Cdd:cd01478 240 KVLVNSTGGHVVLSDSFTTSIFKQSFQR 267
|
|
| Sec23_trunk |
pfam04811 |
Sec23/Sec24 trunk domain; COPII-coated vesicles carry proteins from the endoplasmic reticulum ... |
121-388 |
3.47e-83 |
|
Sec23/Sec24 trunk domain; COPII-coated vesicles carry proteins from the endoplasmic reticulum to the Golgi complex. This vesicular transport can be reconstituted by using three cytosolic components containing five proteins: the small GTPase Sar1p, the Sec23p/24p complex, and the Sec13p/Sec31p complex. This domain is known as the trunk domain and has an alpha/beta vWA fold and forms the dimer interface.
Pssm-ID: 398467 [Multi-domain] Cd Length: 241 Bit Score: 264.50 E-value: 3.47e-83
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 121 PPIFFFVVDLT---SETENLDSLKESIITSLSLLP--PNALIGLITYGNVVQLHDLSSETidrcnvfRGDREYQLEALTE 195
Cdd:pfam04811 3 PPVFLFVIDVSynaIKSGLLAALKESLLQSLDLLPgdPRARVGFITFDSTVHFFNLGSSL-------RQPQMLVVSDLQD 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 196 MLTgqkptgpggaashlpnamnkvtPFSlNRFFLPLEQVEFKLNQLLENLsPDQWSVPagHRPLRATGSALNIASLLLQG 275
Cdd:pfam04811 76 MFL----------------------PLP-DRFLVPLSECRFVLEDLLEQL-PPMFPVT--KRPERCLGPALQAAFLLLKA 129
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 276 CYKniPARIILFASGPGTVAPGLIVNSELKDplrSHHDIDSDHAQHYKKACKFYNQIAQRVAANGHTVDIFAGCYDQIGM 355
Cdd:pfam04811 130 AFT--GGKIMVFQGGLPTVGPGGKLKSRLDE---SHHGTDKEKAKLVKKADKFYKSLAKECVKQGHSVDLFAFSLDYVDV 204
|
250 260 270
....*....|....*....|....*....|....*..
gi 768899196 356 SEMKQLTDSTGGVLLLTDAFS----TAIFKQSYLRLF 388
Cdd:pfam04811 205 ATLGQLSRLTGGQVYLYPSFQadvdGSKFKQDLQRYF 241
|
|
| trunk_domain |
cd01468 |
trunk domain. COPII-coated vesicles carry proteins from the endoplasmic reticulum to the Golgi ... |
121-386 |
7.65e-79 |
|
trunk domain. COPII-coated vesicles carry proteins from the endoplasmic reticulum to the Golgi complex. This vesicular transport can be reconstituted by using three cytosolic components containing five proteins: the small GTPase Sar1p, the Sec23p/24p complex, and the Sec13p/Sec31p complex. This domain is known as the trunk domain and has an alpha/beta vWA fold and forms the dimer interface. Some members of this family possess a partial MIDAS motif that is a characteristic feature of most vWA domain proteins.
Pssm-ID: 238745 [Multi-domain] Cd Length: 239 Bit Score: 252.94 E-value: 7.65e-79
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 121 PPIFFFVVDLTSE---TENLDSLKESIITSLSLLP--PNALIGLITYGNVVQLHDLSSETID-RCNVFRGDREyqlealt 194
Cdd:cd01468 3 PPVFVFVIDVSYEaikEGLLQALKESLLASLDLLPgdPRARVGLITYDSTVHFYNLSSDLAQpKMYVVSDLKD------- 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 195 emltgqkptgpggaashlpnamnkVTPFSLNRFFLPLEQVEFKLNQLLENLSPDQWSVPaGHRPLRATGSALNIASLLLQ 274
Cdd:cd01468 76 ------------------------VFLPLPDRFLVPLSECKKVIHDLLEQLPPMFWPVP-THRPERCLGPALQAAFLLLK 130
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 275 GCYknIPARIILFASGPGTVAPGLIVNSELKDPLRSHhdidsDHAQHYKKACKFYNQIAQRVAANGHTVDIFAGCYDQIG 354
Cdd:cd01468 131 GTF--AGGRIIVFQGGLPTVGPGKLKSREDKEPIRSH-----DEAQLLKPATKFYKSLAKECVKSGICVDLFAFSLDYVD 203
|
250 260 270
....*....|....*....|....*....|....*.
gi 768899196 355 MSEMKQLTDSTGGVLLLTDAFS----TAIFKQSYLR 386
Cdd:cd01468 204 VATLKQLAKSTGGQVYLYDSFQapndGSKFKQDLQR 239
|
|
| Sec23_C |
cd11287 |
C-terminal Actin depolymerization factor-homology domain of Sec23; The C-terminal domain of ... |
616-736 |
5.80e-71 |
|
C-terminal Actin depolymerization factor-homology domain of Sec23; The C-terminal domain of the Sec23 subunit of the coat protein complex II (COPII) is distantly related to gelsolin-like repeats and the actin depolymerizing domains found in cofilin and similar proteins. Sec23 forms a tight complex with Sec24. The cytoplasmic Sec23/24 complex is recruited together with Sar1-GTP and Sec13/31 to induce coat polymerization and membrane deformation in the forming of COPII-coated endoplasmic reticulum vesicles. The function of the Sec23 C-terminal domain is unclear.
Pssm-ID: 200443 [Multi-domain] Cd Length: 121 Bit Score: 227.64 E-value: 5.80e-71
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 616 EDTTNSLIMIQPTLTSFSMEDDPQPVLLDSISVKPNTILLLDTFFFILIYHGEQIAQWRKAGYQDDPQYADFKALLEEPK 695
Cdd:cd11287 1 EDVSNSLIMIQPTLYSYSFNGPPEPVLLDSSSILPDRILLLDTFFHILIYHGETIAQWRKAGYQDQPEYENFKDLLEAPV 80
|
90 100 110 120
....*....|....*....|....*....|....*....|.
gi 768899196 696 LEAAELLVDRFPLPRFIDTEAGGSQARFLLSKLNPSDNYQD 736
Cdd:cd11287 81 DDAQELLQDRFPMPRYIVTEQGGSQARFLLSKVNPSQTHNN 121
|
|
| Sec23_helical |
pfam04815 |
Sec23/Sec24 helical domain; COPII-coated vesicles carry proteins from the endoplasmic ... |
524-623 |
1.93e-32 |
|
Sec23/Sec24 helical domain; COPII-coated vesicles carry proteins from the endoplasmic reticulum to the Golgi complex. This vesicular transport can be reconstituted by using three cytosolic components containing five proteins: the small GTPase Sar1p, the Sec23p/24p complex, and the Sec13p/Sec31p complex. This domain is composed of five alpha helices.
Pssm-ID: 461441 [Multi-domain] Cd Length: 103 Bit Score: 121.07 E-value: 1.93e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 524 DQEAAAVLMARIAVHKAETDDGADVIRWLDRTLIKLCQKYADYNKD--DPQSFRLAPNFSLYPQFTYYLRRSQFLSVFNN 601
Cdd:pfam04815 1 DQEAIAVLLAKKAVEKALSSSLSDAREALDNKLVDILAAYRKYCASssSPGQLILPESLKLLPLYMLALLKSPALRGGNS 80
|
90 100
....*....|....*....|...
gi 768899196 602 SP-DETAFYRHIFTREDTTNSLI 623
Cdd:pfam04815 81 SPsDERAYARHLLLSLPVEELLL 103
|
|
| Sec23_BS |
pfam08033 |
Sec23/Sec24 beta-sandwich domain; |
399-510 |
7.19e-26 |
|
Sec23/Sec24 beta-sandwich domain;
Pssm-ID: 429794 [Multi-domain] Cd Length: 86 Bit Score: 101.84 E-value: 7.19e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 399 AFNGNMAVKTSKDLKVQGLIGHASAVKKTDanniseseigigatsTWKMASLSPYHSYAIFFEIantaansnpmmsaPGS 478
Cdd:pfam08033 1 GFNAVLRVRTSKGLKVSGFIGNFVSRSSGD---------------TWKLPSLDPDTSYAFEFDI-------------DEP 52
|
90 100 110
....*....|....*....|....*....|..
gi 768899196 479 ADRPHLAYTQFITTYQHSSGTNRIRVTTVANQ 510
Cdd:pfam08033 53 LPNGSNAYIQFALLYTHSSGERRIRVTTVALP 84
|
|
| COG5028 |
COG5028 |
Vesicle coat complex COPII, subunit SEC24/subunit SFB2/subunit SFB3 [Intracellular trafficking ... |
5-673 |
1.24e-24 |
|
Vesicle coat complex COPII, subunit SEC24/subunit SFB2/subunit SFB3 [Intracellular trafficking and secretion];
Pssm-ID: 227361 [Multi-domain] Cd Length: 861 Bit Score: 110.27 E-value: 1.24e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 5 TNEDINGVRFTWNVFPSTRSDANSNVVPVGCLYTPLKE-YDELNVAPYN----PVVCSgpHCKSILNPYCVIDPRNSSWS 79
Cdd:COG5028 146 SNCSPKYVRSTMYAIPETNDLLKKSKIPFGLVIRPFLElYPEEDPVPLVedgsIVRCR--RCRSYINPFVQFIEQGRKWR 223
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 80 CPICNSRNHLPPQYTNLSQENMPL-------ELQSTTIEYITNKPVTV----PPIFFFVVDLTSETEN---LDSLKESII 145
Cdd:COG5028 224 CNICRSKNDVPEGFDNPSGPNDPRsdrysrpELKSGVVDFLAPKEYSLrqppPPVYVFLIDVSFEAIKnglVKAAIRAIL 303
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 146 TSLSLLP---PNALIGLITYGNVVQLHDLSSetidrcnvfrGDREYQLEAL-TEmltgqkptgpggaashlpnamNKVTP 221
Cdd:COG5028 304 ENLDQIPnfdPRTKIAIICFDSSLHFFKLSP----------DLDEQMLIVSdLD---------------------EPFLP 352
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 222 FSLNRFFLPLEQVEFKLNQLLENLSpdqwSVPAG-HRPLRATGSALNIASLLLQGCYKNIparIILFASGPgTVAPGliv 300
Cdd:COG5028 353 FPSGLFVLPLKSCKQIIETLLDRVP----RIFQDnKSPKNALGPALKAAKSLIGGTGGKI---IVFLSTLP-NMGIG--- 421
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 301 NSELKDPLRSHHDIDSDhaqhykkacKFYNQIAQRVAANGHTVDIFAGCYDQIGMSEMKQLTDSTGGVLL------LTDA 374
Cdd:COG5028 422 KLQLREDKESSLLSCKD---------SFYKEFAIECSKVGISVDLFLTSEDYIDVATLSHLCRYTGGQTYfypnfsATRP 492
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 375 FSTAIFKQSYLRLfakdeeGYLKMAFNGNMAVKTSKDLKVQGLIGHASavkktdaNNISESEIgigatstwkMASLSPYH 454
Cdd:COG5028 493 NDATKLANDLVSH------LSMEIGYEAVMRVRCSTGLRVSSFYGNFF-------NRSSDLCA---------FSTMPRDT 550
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 455 SYAIFFEIANTAANSNpmmsapgsadrphlAYTQFITTYQHSSGTNRIRVTTVAnqlLPFGTPA--IAASFDQEAAAVLM 532
Cdd:COG5028 551 SLLVEFSIDEKLMTSD--------------VYFQVALLYTLNDGERRIRVVNLS---LPTSSSIreVYASADQLAIACIL 613
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 533 ARIAVHKAETDDGADVIRWLDRTLIKLCQKYADY--NKDDPQSFRLAPNFSLYPQFTYYLRRSQFLSVFNNSPDETAFYR 610
Cdd:COG5028 614 AKKASTKALNSSLKEARVLINKSMVDILKAYKKElvKSNTSTQLPLPANLKLLPLLMLALLKSSAFRSGSTPSDIRISAL 693
|
650 660 670 680 690 700
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 768899196 611 HIFTREDTTNSLIMIQPTLTSF-SMEDD---PQPVLLDSISVKPNTILLLDTFFFILIYHGEQIAQW 673
Cdd:COG5028 694 NRLTSLPLKQLMRNIYPTLYALhDMPIEaglPDEGLLVLPSPINATSSLLESGGLYLIDTGQKIFLW 760
|
|
| zf-Sec23_Sec24 |
pfam04810 |
Sec23/Sec24 zinc finger; COPII-coated vesicles carry proteins from the endoplasmic reticulum ... |
53-92 |
8.41e-15 |
|
Sec23/Sec24 zinc finger; COPII-coated vesicles carry proteins from the endoplasmic reticulum to the Golgi complex. This vesicular transport can be reconstituted by using three cytosolic components containing five proteins: the small GTPase Sar1p, the Sec23p/24p complex, and the Sec13p/Sec31p complex. This domain is found to be zinc binding domain.
Pssm-ID: 461437 [Multi-domain] Cd Length: 38 Bit Score: 68.63 E-value: 8.41e-15
10 20 30 40
....*....|....*....|....*....|....*....|
gi 768899196 53 PVVCSgpHCKSILNPYCVIDPRNSSWSCPICNSRNHLPPQ 92
Cdd:pfam04810 1 PVRCR--RCRAYLNPFCQFDFGGKKWTCNFCGTRNPVPPE 38
|
|
| Gelsolin |
pfam00626 |
Gelsolin repeat; |
638-724 |
1.45e-10 |
|
Gelsolin repeat;
Pssm-ID: 395501 [Multi-domain] Cd Length: 76 Bit Score: 57.70 E-value: 1.45e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 768899196 638 PQPVLLDSISVKPNTILLLDTFFfiliyhgeQIAQWRkaGYQDDPQYADFKALLEEPKLEaaellVDRFPLPRFIDTEAG 717
Cdd:pfam00626 5 PPPVPLSQESLNSGDCYLLDNGF--------TIFLWV--GKGSSLLEKLFAALLAAQLDD-----DERFPLPEVIRVPQG 69
|
....*..
gi 768899196 718 GSQARFL 724
Cdd:pfam00626 70 KEPARFL 76
|
|
| |
