Type II secretion system (T2SS), protein G; The Type II secretion system, also called Secretion-dependent pathway (SDP), is responsible for the transport of proteins across the outer membrane first exported to the periplasm by the Sec or Tat translocon in Gram-negative (diderm) bacteria. The T2SG family includes proteins such as EpsG (P45773) in Vibrio cholera, XcpT also called PddA (Q00514) in Pseudomonas aeruginosa or PulG (P15746)in Klebsiella pneumoniae. The PulG is thought to be anchored in the inner membrane with its C-terminus directed towards the periplasme. Together with other members of the Type II secretion machinery, it is thought to assemble into a pilus-like structure that may function as a dynamic mechanism to push secreted proteins out of the cell. The polypeptide is organized into a long N-terminal alpha-helix followed by a loop region that separates it from a C-terminal anti-parallel beta-sheet.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 752687055  3 EKGFTLVEMLIVLFIISILLLITIPNVTKHNQTIQKKGCEGLQNMVKAQMTAFELDHeGQTPTLADLQSEGYVKKDAvCP 82
Cdd:NF040999  1 VKGFTLIEMLIVLLIISVLLLLFVPNLSKQKESVQEKGCEAVVKVVESQVELYELDH-NKKPSLSELVSEGYITKKQ-CP 78

                ....*
gi 752687055 83 NGKHI 87
Cdd:NF040999 79 NYKDY 83

competence type IV pilus major pilin ComGC; ComGC, encoded in the comG operon, is the major pilin of a type IV pilus involved in natural transformation of monoderm bacteria (those lacking an outer membrane) such as Bacillus subtilis and Streptococcus pneumoniae. In the seed alignment, Bacillus proteins have a pair of Cys residues likely to form a disulfide bond while Streptococcus proteins lack Cys residues.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 752687055  3 EKGFTLVEMLIVLFIISILLLITIPNVTKHNQTIQKKGCEGLQNMVKAQMTAFELDHeGQTPTLADLQSEGYVKKDAvCP 82
Cdd:NF040999  1 VKGFTLIEMLIVLLIISVLLLLFVPNLSKQKESVQEKGCEAVVKVVESQVELYELDH-NKKPSLSELVSEGYITKKQ-CP 78

                ....*
gi 752687055 83 NGKHI 87
Cdd:NF040999 79 NYKDY 83

competence type IV pilus major pilin ComGC; ComGC, encoded in the comG operon, is the major pilin of a type IV pilus involved in natural transformation of monoderm bacteria (those lacking an outer membrane) such as Bacillus subtilis and Streptococcus pneumoniae. In the seed alignment, Bacillus proteins have a pair of Cys residues likely to form a disulfide bond while Streptococcus proteins lack Cys residues.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 752687055  3 EKGFTLVEMLIVLFIISILLLITIPNVTKHNQTIQKKGCEGLQNMVKAQMTAFELDHeGQTPTLADLQSEGYVKKDAvCP 82
Cdd:NF040999  1 VKGFTLIEMLIVLLIISVLLLLFVPNLSKQKESVQEKGCEAVVKVVESQVELYELDH-NKKPSLSELVSEGYITKKQ-CP 78

                ....*
gi 752687055 83 NGKHI 87
Cdd:NF040999 79 NYKDY 83