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Conserved domains on  [gi|71147043|gb|AAZ27516|]
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putative polysaccharide biosynthesis glycosyltransferase [Colwellia psychrerythraea 34H]

Protein Classification

sugar transferase( domain architecture ID 10005412)

sugar transferase catalyzes the transfer of a sugar from a donor such as UDP-glucose or UDP-galactose, to a lipid carrier such as undecaprenyl phosphate

EC:  2.-.-.-
Gene Ontology:  GO:0016780|GO:0000271|GO:0005886
PubMed:  29769739
SCOP:  4007826

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
WcaJ COG2148
Sugar transferase involved in LPS biosynthesis (colanic, teichoic acid) [Cell wall/membrane ...
 1-176 7.15e-65

Sugar transferase involved in LPS biosynthesis (colanic, teichoic acid) [Cell wall/membrane/envelope biogenesis];


:

Pssm-ID: 441751 [Multi-domain]  Cd Length: 322  Bit Score: 202.27  E-value: 7.15e-65
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71147043   1 MTLLLIRILDFIFSFFGLLFACPFLIILTIIGYFDTGSPI-FIQERVGRNKKPFNLIKFRTMTVGTASVASH---LASSA 76
Cdd:COG2148 134 YQRVLKRLFDIVLALLGLILLSPLLLLIALAIKLDSGGPVfFRQERVGRNGRPFTIYKFRTMRVDAEKLLGAvfkLKNDP 213

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71147043  77 SITNFGSFLRKTKLDELPQLWNVLKGEMSLVGPRPNLFNQTELVLERESRGVYNVRPGVTGLSQVNEIDMSTPILLAKTD 156
Cdd:COG2148 214 RITRVGRFLRKTSLDELPQLWNVLKGDMSLVGPRPELPEEVELYEEEEYRRRLLVKPGITGLAQVNGRNGETFEERVELD 293

                       170       180
                ....*....|....*....|
gi 71147043 157 EQMILTLTISYYFKYILQTV 176
Cdd:COG2148 294 LYYIENWSLWLDLKILLKTV 313
 
Name Accession Description Interval E-value
WcaJ COG2148
Sugar transferase involved in LPS biosynthesis (colanic, teichoic acid) [Cell wall/membrane ...
 1-176 7.15e-65

Sugar transferase involved in LPS biosynthesis (colanic, teichoic acid) [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441751 [Multi-domain]  Cd Length: 322  Bit Score: 202.27  E-value: 7.15e-65
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71147043   1 MTLLLIRILDFIFSFFGLLFACPFLIILTIIGYFDTGSPI-FIQERVGRNKKPFNLIKFRTMTVGTASVASH---LASSA 76
Cdd:COG2148 134 YQRVLKRLFDIVLALLGLILLSPLLLLIALAIKLDSGGPVfFRQERVGRNGRPFTIYKFRTMRVDAEKLLGAvfkLKNDP 213

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71147043  77 SITNFGSFLRKTKLDELPQLWNVLKGEMSLVGPRPNLFNQTELVLERESRGVYNVRPGVTGLSQVNEIDMSTPILLAKTD 156
Cdd:COG2148 214 RITRVGRFLRKTSLDELPQLWNVLKGDMSLVGPRPELPEEVELYEEEEYRRRLLVKPGITGLAQVNGRNGETFEERVELD 293

                       170       180
                ....*....|....*....|
gi 71147043 157 EQMILTLTISYYFKYILQTV 176
Cdd:COG2148 294 LYYIENWSLWLDLKILLKTV 313
Bac_transf pfam02397
Bacterial sugar transferase; This Pfam family represents a conserved region from a number of ...
 7-176 1.41e-61

Bacterial sugar transferase; This Pfam family represents a conserved region from a number of different bacterial sugar transferases, involved in diverse biosynthesis pathways.


Pssm-ID: 460547 [Multi-domain]  Cd Length: 180  Bit Score: 188.72  E-value: 1.41e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71147043     7 RILDFIFSFFGLLFACPFLIILTIIGYFDTGSPI-FIQERVGRNKKPFNLIKFRTMTVGTASVASHLASS--ASITNFGS 83
Cdd:pfam02397   2 RLFDIVLSLLGLILLSPLLLLIAIAIKLLSGGPVfFRQERVGKNGKPFTIYKFRTMVVDAEKRGPLFKLKndPRITRVGR 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71147043    84 FLRKTKLDELPQLWNVLKGEMSLVGPRPNLFNQTELVLERESRGVYNVRPGVTGLSQVN----EIDMSTPILLaktDEQM 159
Cdd:pfam02397  82 FLRKTSLDELPQLINVLKGDMSLVGPRPELPEFEYELYERDQRRRLSVKPGITGLAQVNggrsELSFEEKLEL---DLYY 158

                         170
                  ....*....|....*..
gi 71147043   160 ILTLTISYYFKYILQTV 176
Cdd:pfam02397 159 IENWSLWLDLKILLKTV 175
EPS_sugtrans TIGR03025
exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase; Members of this family ...
 7-142 1.52e-41

exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase; Members of this family are generally found near other genes involved in the biosynthesis of a variety of exopolysaccharides. These proteins consist of two fused domains, an N-terminal hydrophobic domain of generally low conservation and a highly conserved C-terminal sugar transferase domain (pfam02397). Characterized and partially characterized members of this subfamily include Salmonella WbaP (originally RfbP), E. coli WcaJ, Methylobacillus EpsB, Xanthomonas GumD, Vibrio CpsA, Erwinia AmsG, Group B Streptococcus CpsE (originally CpsD), and Streptococcus suis Cps2E. Each of these is believed to act in transferring the sugar from, for instance, UDP-glucose or UDP-galactose, to a lipid carrier such as undecaprenyl phosphate as the first (priming) step in the synthesis of an oligosaccharide "block". This function is encoded in the C-terminal domain. The liposaccharide is believed to be subsequently transferred through a "flippase" function from the cytoplasmic to the periplasmic face of the inner membrane by the N-terminal domain. Certain closely related transferase enzymes, such as Sinorhizobium ExoY and Lactococcus EpsD, lack the N-terminal domain and are not found by this model.


Pssm-ID: 274398 [Multi-domain]  Cd Length: 445  Bit Score: 144.65  E-value: 1.52e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71147043     7 RILDFIFSFFGLLFACPFLIILTIIGYFDTGSPI-FIQERVGRNKKPFNLIKFRTMTVGtASVASHLASSAS----ITNF 81
Cdd:TIGR03025 259 RLFDIVLSLLALLLLSPLMLAIALAIKLDSPGPVfFRQERVGLNGKPFTVYKFRSMRVD-AEEGGGPVQATKndprITRV 337

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 71147043    82 GSFLRKTKLDELPQLWNVLKGEMSLVGPRPNL--FNQ--TELVLERESRgvYNVRPGVTGLSQVN 142
Cdd:TIGR03025 338 GRFLRRTSLDELPQLFNVLKGDMSLVGPRPERpaEVEkyEQEIPGYMLR--HKVKPGITGWAQVS 400
PRK15204 PRK15204
undecaprenyl-phosphate galactose phosphotransferase; Provisional
 4-150 1.88e-25

undecaprenyl-phosphate galactose phosphotransferase; Provisional


Pssm-ID: 185126 [Multi-domain]  Cd Length: 476  Bit Score: 101.62  E-value: 1.88e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71147043    4 LLIRILDFIFSFFGLLFACPFLIILTIIGYFDTGSPIFIQERVGRNKKPFNLIKFRTMTVGTASVASHLASS-------- 75
Cdd:PRK15204 278 FLKRTFDIVCSIMILIIASPLMIYLWYKVTRDGGPAIYGHQRVGRHGKLFPCYKFRSMVMNSQEVLKELLANdpiaraew 357

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71147043   76 ---------ASITNFGSFLRKTKLDELPQLWNVLKGEMSLVGPRPNLFNQtelvLERESRGV---YNVRPGVTGLSQV-- 141
Cdd:PRK15204 358 ekdfklkndPRITAVGRFIRKTSLDELPQLFNVLKGDMSLVGPRPIVSDE----LERYCDDVdyyLMAKPGMTGLWQVsg 433

                        170
                 ....*....|
gi 71147043  142 -NEIDMSTPI 150
Cdd:PRK15204 434 rNDVDYDTRV 443
 
Name Accession Description Interval E-value
WcaJ COG2148
Sugar transferase involved in LPS biosynthesis (colanic, teichoic acid) [Cell wall/membrane ...
 1-176 7.15e-65

Sugar transferase involved in LPS biosynthesis (colanic, teichoic acid) [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441751 [Multi-domain]  Cd Length: 322  Bit Score: 202.27  E-value: 7.15e-65
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71147043   1 MTLLLIRILDFIFSFFGLLFACPFLIILTIIGYFDTGSPI-FIQERVGRNKKPFNLIKFRTMTVGTASVASH---LASSA 76
Cdd:COG2148 134 YQRVLKRLFDIVLALLGLILLSPLLLLIALAIKLDSGGPVfFRQERVGRNGRPFTIYKFRTMRVDAEKLLGAvfkLKNDP 213

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71147043  77 SITNFGSFLRKTKLDELPQLWNVLKGEMSLVGPRPNLFNQTELVLERESRGVYNVRPGVTGLSQVNEIDMSTPILLAKTD 156
Cdd:COG2148 214 RITRVGRFLRKTSLDELPQLWNVLKGDMSLVGPRPELPEEVELYEEEEYRRRLLVKPGITGLAQVNGRNGETFEERVELD 293

                       170       180
                ....*....|....*....|
gi 71147043 157 EQMILTLTISYYFKYILQTV 176
Cdd:COG2148 294 LYYIENWSLWLDLKILLKTV 313
Bac_transf pfam02397
Bacterial sugar transferase; This Pfam family represents a conserved region from a number of ...
 7-176 1.41e-61

Bacterial sugar transferase; This Pfam family represents a conserved region from a number of different bacterial sugar transferases, involved in diverse biosynthesis pathways.


Pssm-ID: 460547 [Multi-domain]  Cd Length: 180  Bit Score: 188.72  E-value: 1.41e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71147043     7 RILDFIFSFFGLLFACPFLIILTIIGYFDTGSPI-FIQERVGRNKKPFNLIKFRTMTVGTASVASHLASS--ASITNFGS 83
Cdd:pfam02397   2 RLFDIVLSLLGLILLSPLLLLIAIAIKLLSGGPVfFRQERVGKNGKPFTIYKFRTMVVDAEKRGPLFKLKndPRITRVGR 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71147043    84 FLRKTKLDELPQLWNVLKGEMSLVGPRPNLFNQTELVLERESRGVYNVRPGVTGLSQVN----EIDMSTPILLaktDEQM 159
Cdd:pfam02397  82 FLRKTSLDELPQLINVLKGDMSLVGPRPELPEFEYELYERDQRRRLSVKPGITGLAQVNggrsELSFEEKLEL---DLYY 158

                         170
                  ....*....|....*..
gi 71147043   160 ILTLTISYYFKYILQTV 176
Cdd:pfam02397 159 IENWSLWLDLKILLKTV 175
EPS_sugtrans TIGR03025
exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase; Members of this family ...
 7-142 1.52e-41

exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase; Members of this family are generally found near other genes involved in the biosynthesis of a variety of exopolysaccharides. These proteins consist of two fused domains, an N-terminal hydrophobic domain of generally low conservation and a highly conserved C-terminal sugar transferase domain (pfam02397). Characterized and partially characterized members of this subfamily include Salmonella WbaP (originally RfbP), E. coli WcaJ, Methylobacillus EpsB, Xanthomonas GumD, Vibrio CpsA, Erwinia AmsG, Group B Streptococcus CpsE (originally CpsD), and Streptococcus suis Cps2E. Each of these is believed to act in transferring the sugar from, for instance, UDP-glucose or UDP-galactose, to a lipid carrier such as undecaprenyl phosphate as the first (priming) step in the synthesis of an oligosaccharide "block". This function is encoded in the C-terminal domain. The liposaccharide is believed to be subsequently transferred through a "flippase" function from the cytoplasmic to the periplasmic face of the inner membrane by the N-terminal domain. Certain closely related transferase enzymes, such as Sinorhizobium ExoY and Lactococcus EpsD, lack the N-terminal domain and are not found by this model.


Pssm-ID: 274398 [Multi-domain]  Cd Length: 445  Bit Score: 144.65  E-value: 1.52e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71147043     7 RILDFIFSFFGLLFACPFLIILTIIGYFDTGSPI-FIQERVGRNKKPFNLIKFRTMTVGtASVASHLASSAS----ITNF 81
Cdd:TIGR03025 259 RLFDIVLSLLALLLLSPLMLAIALAIKLDSPGPVfFRQERVGLNGKPFTVYKFRSMRVD-AEEGGGPVQATKndprITRV 337

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 71147043    82 GSFLRKTKLDELPQLWNVLKGEMSLVGPRPNL--FNQ--TELVLERESRgvYNVRPGVTGLSQVN 142
Cdd:TIGR03025 338 GRFLRRTSLDELPQLFNVLKGDMSLVGPRPERpaEVEkyEQEIPGYMLR--HKVKPGITGWAQVS 400
WcaJ_sugtrans TIGR03023
Undecaprenyl-phosphate glucose phosphotransferase; This family of proteins encompasses the E. ...
 7-142 1.17e-38

Undecaprenyl-phosphate glucose phosphotransferase; This family of proteins encompasses the E. coli WcaJ protein involved in colanic acid biosynthesis, the Methylobacillus EpsB protein involved in methanolan biosynthesis, as well as the GumD protein involved in the biosynthesis of xanthan. All of these are closely related to the well-characterized WbaP (formerly RfbP) protein, which is the first enzyme in O-antigen biosynthesis in Salmonella typhimurium. The enzyme transfers galactose from UDP-galactose (NOTE: not glucose) to a polyprenyl carrier (utilizing the highly conserved C-terminal sugar transferase domain, pfam02397) a reaction which takes place at the cytoplasmic face of the inner membrane. The N-terminal hydrophobic domain is then believed to facilitate the "flippase" function of transferring the liposaccharide unit from the cytoplasmic face to the periplasmic face of the inner membrane. Most of these genes are found within large operons dedicated to the production of complex exopolysaccharides such as the enterobacterial O-antigen. Colanic acid biosynthesis utilizes a glucose-undecaprenyl carrier, knockout of EpsB abolishes incorporation of UDP-glucose into the lipid phase, and the C-terminal portion of GumD has been shown to be responsible for the glucosyl-1-transferase activity.


Pssm-ID: 274396 [Multi-domain]  Cd Length: 450  Bit Score: 137.33  E-value: 1.17e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71147043     7 RILDFIFSFFGLLFACPFLIILTIIGYFDtgSP---IFIQERVGRNKKPFNLIKFRTMTVGT--ASVASHLASSASITNF 81
Cdd:TIGR03023 261 RAFDIVLALLVLLLLSPLLLLIAIAIKLT--SPgpvLFRQERYGLDGRPFMVYKFRSMRVHAegDGVTQATRNDPRVTRV 338

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 71147043    82 GSFLRKTKLDELPQLWNVLKGEMSLVGPRPN--LFNQT--ELVLERESRgvYNVRPGVTGLSQVN 142
Cdd:TIGR03023 339 GAFLRRTSLDELPQFFNVLKGDMSIVGPRPHavAHNEQyrKLIPGYMLR--HKVKPGITGWAQVN 401
WbaP_sugtrans TIGR03022
Undecaprenyl-phosphate galactose phosphotransferase, WbaP; The WbaP (formerly RfbP) protein ...
 2-143 2.33e-38

Undecaprenyl-phosphate galactose phosphotransferase, WbaP; The WbaP (formerly RfbP) protein has been characterized as the first enzyme in O-antigen biosynthesis in Salmonella typhimurium. The enzyme transfers galactose from UDP-galactose to a polyprenyl carrier (utilizing the highly conserved C-terminal sugar transferase domain, pfam02397) a reaction which takes place at the cytoplasmic face of the inner membrane. The N-terminal hydrophobic domain is then believed to facilitate the "flippase" function of transferring the liposaccharide unit from the cytoplasmic face to the periplasmic face of the inner membrane. This model includes the enterobacterial enzymes, where the function is presumed to be identical to the S. typhimurium enzyme as well as a somewhat broader group which are likely to catalyze the same or highly similar reactions based on a phylogenetic tree-building analysis of the broader sugar transferase family. Most of these genes are found within large operons dedicated to the production of complex exopolysaccharides such as the enterobacterial O-antigen. The most likely heterogeneity would be in the precise nature of the sugar molecule transferred.


Pssm-ID: 274395 [Multi-domain]  Cd Length: 456  Bit Score: 136.72  E-value: 2.33e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71147043     2 TLLLIRILDFIFSFFGLLFACPFLIILTIIGYFDTGSP-IFIQERVGRNKKPFNLIKFRTMTVGTASV-ASHLASSAS-- 77
Cdd:TIGR03022 255 ARLIKRTLDLVLSLLALPLLLPLLLVIALLIRLDSKGPaFYKQERVGRNGKLFKCYKFRTMVMNSDQVlEELLAADPElr 334

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71147043    78 --------------ITNFGSFLRKTKLDELPQLWNVLKGEMSLVGPRPNLFNQTELVLErESRGVYNVRPGVTGLSQVNE 143
Cdd:TIGR03022 335 aeweeyhklrndprITRIGKFLRKTSLDELPQLWNVLKGDMSLVGPRPYLTSELSRYGE-ALELYLRVRPGITGLWQVSG 413
PRK15204 PRK15204
undecaprenyl-phosphate galactose phosphotransferase; Provisional
 4-150 1.88e-25

undecaprenyl-phosphate galactose phosphotransferase; Provisional


Pssm-ID: 185126 [Multi-domain]  Cd Length: 476  Bit Score: 101.62  E-value: 1.88e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71147043    4 LLIRILDFIFSFFGLLFACPFLIILTIIGYFDTGSPIFIQERVGRNKKPFNLIKFRTMTVGTASVASHLASS-------- 75
Cdd:PRK15204 278 FLKRTFDIVCSIMILIIASPLMIYLWYKVTRDGGPAIYGHQRVGRHGKLFPCYKFRSMVMNSQEVLKELLANdpiaraew 357

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71147043   76 ---------ASITNFGSFLRKTKLDELPQLWNVLKGEMSLVGPRPNLFNQtelvLERESRGV---YNVRPGVTGLSQV-- 141
Cdd:PRK15204 358 ekdfklkndPRITAVGRFIRKTSLDELPQLFNVLKGDMSLVGPRPIVSDE----LERYCDDVdyyLMAKPGMTGLWQVsg 433

                        170
                 ....*....|
gi 71147043  142 -NEIDMSTPI 150
Cdd:PRK15204 434 rNDVDYDTRV 443
PRK10124 PRK10124
putative UDP-glucose lipid carrier transferase; Provisional
 4-142 2.58e-23

putative UDP-glucose lipid carrier transferase; Provisional


Pssm-ID: 182254 [Multi-domain]  Cd Length: 463  Bit Score: 95.56  E-value: 2.58e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71147043    4 LLIRILDFIFSFFGLLFACPFLIILTIIGYFDTGSP-IFIQERVGRNKKPFNLIKFRTMTV--GTASVASHLASSASITN 80
Cdd:PRK10124 271 LLKRAEDIVLASLILLLISPVLCCIALAVKLSSPGPvIFRQTRYGMDGKPIKVWKFRSMKVmeNDKVVTQATQNDPRVTK 350

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 71147043   81 FGSFLRKTKLDELPQLWNVLKGEMSLVGPRPNLFNQTE----LVLERESRgvYNVRPGVTGLSQVN 142
Cdd:PRK10124 351 VGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEqyrqLIEGYMLR--HKVKPGITGWAQIN 414
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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