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Conserved domains on  [gi|70870669|gb|EAN84963|]
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hypothetical protein, conserved [Trypanosoma cruzi]

Protein Classification

P-loop NTPase family protein( domain architecture ID 1562424)

P-loop NTPase (nucleoside triphosphate hydrolase) family protein contains two conserved sequence signatures, the Walker A motif (the P-loop proper) and Walker B motif which bind, respectively, the beta and gamma phosphate moieties of the bound nucleotide (typically ATP or GTP), and a Mg(2+) cation

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Roc super family cl48019
Ras of Complex, Roc, domain of DAPkinase; Roc, or Ras of Complex, proteins are mitochondrial ...
 63-195 2.67e-03

Ras of Complex, Roc, domain of DAPkinase; Roc, or Ras of Complex, proteins are mitochondrial Rho proteins (Miro-1, and Miro-2) and atypical Rho GTPases. Full-length proteins have a unique domain organization, with tandem GTP-binding domains and two EF hand domains (pfam00036) that may bind calcium. They are also larger than classical small GTPases. It has been proposed that they are involved in mitochondrial homeostasis and apoptosis.


The actual alignment was detected with superfamily member pfam08477:

Pssm-ID: 462490 [Multi-domain]  Cd Length: 114  Bit Score: 37.87  E-value: 2.67e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70870669    63 KAVVRGMRRTGKSTIVSRLCGCSPPTSYIPSseITADTLFYRGTagrTGNCGGGGAKVELWDvvdegisTS-QSASITTH 141
Cdd:pfam08477   1 KVVLLGDSGVGKTSLLKRFVDDTFDPKYKST--IGVDFKTKTVL---ENDDNGKKIKLNIWD-------TAgQERFRSLH 68

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....
gi 70870669   142 PTladarsidVYRGCHLAAFVIDRTRKETLDYAVREARHVPPDTCVLFILNFYD 195
Cdd:pfam08477  69 PF--------YYRGAAAALLVYDSRTFSNLKYWLRELKKYAGNSPVILVGNKID 114
 
Name Accession Description Interval E-value
Roc pfam08477
Ras of Complex, Roc, domain of DAPkinase; Roc, or Ras of Complex, proteins are mitochondrial ...
 63-195 2.67e-03

Ras of Complex, Roc, domain of DAPkinase; Roc, or Ras of Complex, proteins are mitochondrial Rho proteins (Miro-1, and Miro-2) and atypical Rho GTPases. Full-length proteins have a unique domain organization, with tandem GTP-binding domains and two EF hand domains (pfam00036) that may bind calcium. They are also larger than classical small GTPases. It has been proposed that they are involved in mitochondrial homeostasis and apoptosis.


Pssm-ID: 462490 [Multi-domain]  Cd Length: 114  Bit Score: 37.87  E-value: 2.67e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70870669    63 KAVVRGMRRTGKSTIVSRLCGCSPPTSYIPSseITADTLFYRGTagrTGNCGGGGAKVELWDvvdegisTS-QSASITTH 141
Cdd:pfam08477   1 KVVLLGDSGVGKTSLLKRFVDDTFDPKYKST--IGVDFKTKTVL---ENDDNGKKIKLNIWD-------TAgQERFRSLH 68

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....
gi 70870669   142 PTladarsidVYRGCHLAAFVIDRTRKETLDYAVREARHVPPDTCVLFILNFYD 195
Cdd:pfam08477  69 PF--------YYRGAAAALLVYDSRTFSNLKYWLRELKKYAGNSPVILVGNKID 114
Ras_like_GTPase cd00882
Rat sarcoma (Ras)-like superfamily of small guanosine triphosphatases (GTPases); Ras-like ...
 65-259 6.12e-03

Rat sarcoma (Ras)-like superfamily of small guanosine triphosphatases (GTPases); Ras-like GTPase superfamily. The Ras-like superfamily of small GTPases consists of several families with an extremely high degree of structural and functional similarity. The Ras superfamily is divided into at least four families in eukaryotes: the Ras, Rho, Rab, and Sar1/Arf families. This superfamily also includes proteins like the GTP translation factors, Era-like GTPases, and G-alpha chain of the heterotrimeric G proteins. Members of the Ras superfamily regulate a wide variety of cellular functions: the Ras family regulates gene expression, the Rho family regulates cytoskeletal reorganization and gene expression, the Rab and Sar1/Arf families regulate vesicle trafficking, and the Ran family regulates nucleocytoplasmic transport and microtubule organization. The GTP translation factor family regulates initiation, elongation, termination, and release in translation, and the Era-like GTPase family regulates cell division, sporulation, and DNA replication. Members of the Ras superfamily are identified by the GTP binding site, which is made up of five characteristic sequence motifs, and the switch I and switch II regions.


Pssm-ID: 206648 [Multi-domain]  Cd Length: 161  Bit Score: 37.82  E-value: 6.12e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70870669  65 VVRGMRRTGKSTIVSRLCGcSPPTSYIPSSEITADTLFYRGTAGRTGNcggggaKVELWDVvdEGISTSQsasitthPTL 144
Cdd:cd00882   1 VVVGRGGVGKSSLLNALLG-GEVGEVSDVPGTTRDPDVYVKELDKGKV------KLVLVDT--PGLDEFG-------GLG 64

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70870669 145 ADARSIDVYRGCHLAAFVIDRTRKETLDYA--VREARHVPPDTCVLFILNFYDAPRDAHVVsdgDVDAACKSIRRATTPM 222
Cdd:cd00882  65 REELARLLLRGADLILLVVDSTDRESEEDAklLILRRLRKEGIPIILVGNKIDLLEEREVE---ELLRLEELAKILGVPV 141

                       170       180       190
                ....*....|....*....|....*....|....*..
gi 70870669 223 ILaasagrlppeeysisatwvsISAATGHGMELLRNA 259
Cdd:cd00882 142 FE--------------------VSAKTGEGVDELFEK 158
 
Name Accession Description Interval E-value
Roc pfam08477
Ras of Complex, Roc, domain of DAPkinase; Roc, or Ras of Complex, proteins are mitochondrial ...
 63-195 2.67e-03

Ras of Complex, Roc, domain of DAPkinase; Roc, or Ras of Complex, proteins are mitochondrial Rho proteins (Miro-1, and Miro-2) and atypical Rho GTPases. Full-length proteins have a unique domain organization, with tandem GTP-binding domains and two EF hand domains (pfam00036) that may bind calcium. They are also larger than classical small GTPases. It has been proposed that they are involved in mitochondrial homeostasis and apoptosis.


Pssm-ID: 462490 [Multi-domain]  Cd Length: 114  Bit Score: 37.87  E-value: 2.67e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70870669    63 KAVVRGMRRTGKSTIVSRLCGCSPPTSYIPSseITADTLFYRGTagrTGNCGGGGAKVELWDvvdegisTS-QSASITTH 141
Cdd:pfam08477   1 KVVLLGDSGVGKTSLLKRFVDDTFDPKYKST--IGVDFKTKTVL---ENDDNGKKIKLNIWD-------TAgQERFRSLH 68

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....
gi 70870669   142 PTladarsidVYRGCHLAAFVIDRTRKETLDYAVREARHVPPDTCVLFILNFYD 195
Cdd:pfam08477  69 PF--------YYRGAAAALLVYDSRTFSNLKYWLRELKKYAGNSPVILVGNKID 114
Ras_like_GTPase cd00882
Rat sarcoma (Ras)-like superfamily of small guanosine triphosphatases (GTPases); Ras-like ...
 65-259 6.12e-03

Rat sarcoma (Ras)-like superfamily of small guanosine triphosphatases (GTPases); Ras-like GTPase superfamily. The Ras-like superfamily of small GTPases consists of several families with an extremely high degree of structural and functional similarity. The Ras superfamily is divided into at least four families in eukaryotes: the Ras, Rho, Rab, and Sar1/Arf families. This superfamily also includes proteins like the GTP translation factors, Era-like GTPases, and G-alpha chain of the heterotrimeric G proteins. Members of the Ras superfamily regulate a wide variety of cellular functions: the Ras family regulates gene expression, the Rho family regulates cytoskeletal reorganization and gene expression, the Rab and Sar1/Arf families regulate vesicle trafficking, and the Ran family regulates nucleocytoplasmic transport and microtubule organization. The GTP translation factor family regulates initiation, elongation, termination, and release in translation, and the Era-like GTPase family regulates cell division, sporulation, and DNA replication. Members of the Ras superfamily are identified by the GTP binding site, which is made up of five characteristic sequence motifs, and the switch I and switch II regions.


Pssm-ID: 206648 [Multi-domain]  Cd Length: 161  Bit Score: 37.82  E-value: 6.12e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70870669  65 VVRGMRRTGKSTIVSRLCGcSPPTSYIPSSEITADTLFYRGTAGRTGNcggggaKVELWDVvdEGISTSQsasitthPTL 144
Cdd:cd00882   1 VVVGRGGVGKSSLLNALLG-GEVGEVSDVPGTTRDPDVYVKELDKGKV------KLVLVDT--PGLDEFG-------GLG 64

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70870669 145 ADARSIDVYRGCHLAAFVIDRTRKETLDYA--VREARHVPPDTCVLFILNFYDAPRDAHVVsdgDVDAACKSIRRATTPM 222
Cdd:cd00882  65 REELARLLLRGADLILLVVDSTDRESEEDAklLILRRLRKEGIPIILVGNKIDLLEEREVE---ELLRLEELAKILGVPV 141

                       170       180       190
                ....*....|....*....|....*....|....*..
gi 70870669 223 ILaasagrlppeeysisatwvsISAATGHGMELLRNA 259
Cdd:cd00882 142 FE--------------------VSAKTGEGVDELFEK 158
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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