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Conserved domains on  [gi|68563388|gb|AAH99661|]
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ZNF124 protein [Homo sapiens]

Protein Classification

KRAB domain-containing zinc finger protein( domain architecture ID 12016853)

KRAB (Kruppel-associated box) domain-containing zinc finger protein (KRAB-ZFP) plays important roles in cell differentiation and organ development and in regulating viral replication and transcription

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
KRAB pfam01352
KRAB box; The KRAB domain (or Kruppel-associated box) is present in about a third of zinc ...
 12-53 2.02e-25

KRAB box; The KRAB domain (or Kruppel-associated box) is present in about a third of zinc finger proteins containing C2H2 fingers. The KRAB domain is found to be involved in protein-protein interactions. The KRAB domain is generally encoded by two exons. The regions coded by the two exons are known as KRAB-A and KRAB-B. The A box plays an important role in repression by binding to corepressors, while the B box is thought to enhance this repression brought about by the A box. KRAB-containing proteins are thought to have critical functions in cell proliferation and differentiation, apoptosis and neoplastic transformation.


:

Pssm-ID: 460171  Cd Length: 42  Bit Score: 96.39  E-value: 2.02e-25
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 68563388    12 SVAFEDVAVNFTQEEWALLDPSQKNLYRDVMQETFRNLASIG 53
Cdd:pfam01352   1 SVTFEDVAVDFTQEEWALLDPAQRNLYRDVMLENYRNLVSLG 42
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
176-338 7.56e-07

FOG: Zn-finger [General function prediction only];


:

Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 50.46  E-value: 7.56e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 68563388 176 KRYECKQCGKAFSRSSHLRDHERT--HTGE--KPYECKH--CGKAFRYSNCLHYHERTHTGEKPYVCM--ECGKAFSCLS 247
Cdd:COG5048 288 LPIKSKQCNISFSRSSPLTRHLRSvnHSGEslKPFSCPYslCGKLFSRNDALKRHILLHTSISPAKEKllNSSSKFSPLL 367

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 68563388 248 ------SLQGHIKAHAGEEPYPCKQ-CGKAFRYASSLQKHEKTHIAQKPYVCNN--CGKGFRCSSSLRDHERTHTGEKPY 318
Cdd:COG5048 368 nneppqSLQQYKDLKNDKKSETLSNsCIRNFKRDSNLSLHIITHLSFRPYNCKNppCSKSFNRHYNLIPHKKIHTNHAPL 447

                       170       180
                ....*....|....*....|
gi 68563388 319 ECQKCGKaFSRASTLWKHKK 338
Cdd:COG5048 448 LCSILKS-FRRDLDLSNHGK 466
 
Name Accession Description Interval E-value
KRAB pfam01352
KRAB box; The KRAB domain (or Kruppel-associated box) is present in about a third of zinc ...
 12-53 2.02e-25

KRAB box; The KRAB domain (or Kruppel-associated box) is present in about a third of zinc finger proteins containing C2H2 fingers. The KRAB domain is found to be involved in protein-protein interactions. The KRAB domain is generally encoded by two exons. The regions coded by the two exons are known as KRAB-A and KRAB-B. The A box plays an important role in repression by binding to corepressors, while the B box is thought to enhance this repression brought about by the A box. KRAB-containing proteins are thought to have critical functions in cell proliferation and differentiation, apoptosis and neoplastic transformation.


Pssm-ID: 460171  Cd Length: 42  Bit Score: 96.39  E-value: 2.02e-25
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 68563388    12 SVAFEDVAVNFTQEEWALLDPSQKNLYRDVMQETFRNLASIG 53
Cdd:pfam01352   1 SVTFEDVAVDFTQEEWALLDPAQRNLYRDVMLENYRNLVSLG 42
KRAB smart00349
krueppel associated box;
13-55 3.01e-23

krueppel associated box;


Pssm-ID: 214630 [Multi-domain]  Cd Length: 61  Bit Score: 91.11  E-value: 3.01e-23
                           10        20        30        40
                   ....*....|....*....|....*....|....*....|...
gi 68563388     13 VAFEDVAVNFTQEEWALLDPSQKNLYRDVMQETFRNLASIGNK 55
Cdd:smart00349   1 VTFEDVAVYFTQEEWEQLDPAQKNLYRDVMLENYSNLVSLGFQ 43
KRAB_A-box cd07765
KRAB (Kruppel-associated box) domain -A box; The KRAB domain is a transcription repression ...
 13-52 6.39e-21

KRAB (Kruppel-associated box) domain -A box; The KRAB domain is a transcription repression module, found in a subgroup of the zinc finger proteins (ZFPs) of the C2H2 family, KRAB-ZFPs. KRAB-ZFPs comprise the largest group of transcriptional regulators in mammals, and are only found in tetrapods. These proteins have been shown to play important roles in cell differentiation and organ development, and in regulating viral replication and transcription. A KRAB domain may consist of an A-box, or of an A-box plus either a B-box, a divergent B-box (b), or a C-box. Only the A-box is included in this model. The A-box is needed for repression, the B- and C- boxes are not. KRAB-ZFPs have one or two KRAB domains at their amino-terminal end, and multiple C2H2 zinc finger motifs at their C-termini. Some KRAB-ZFPs also contain a SCAN domain which mediates homo- and hetero-oligomerization. The KRAB domain is a protein-protein interaction module which represses transcription through recruiting corepressors. A key mechanism appears to be the following: KRAB-AFPs tethered to DNA recruit, via their KRAB domain, the repressor KAP1 (KRAB-associated protein-1, also known as transcription intermediary factor 1 beta , KRAB-A interacting protein , and tripartite motif protein 28). The KAP1/ KRAB-AFP complex in turn recruits the heterochromatin protein 1 (HP1) family, and other chromatin modulating proteins, leading to transcriptional repression through heterochromatin formation.


Pssm-ID: 143639  Cd Length: 40  Bit Score: 84.14  E-value: 6.39e-21
                        10        20        30        40
                ....*....|....*....|....*....|....*....|
gi 68563388  13 VAFEDVAVNFTQEEWALLDPSQKNLYRDVMQETFRNLASI 52
Cdd:cd07765   1 VTFEDVAVYFSQEEWELLDPAQRDLYRDVMLENYENLVSL 40
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
176-338 7.56e-07

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 50.46  E-value: 7.56e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 68563388 176 KRYECKQCGKAFSRSSHLRDHERT--HTGE--KPYECKH--CGKAFRYSNCLHYHERTHTGEKPYVCM--ECGKAFSCLS 247
Cdd:COG5048 288 LPIKSKQCNISFSRSSPLTRHLRSvnHSGEslKPFSCPYslCGKLFSRNDALKRHILLHTSISPAKEKllNSSSKFSPLL 367

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 68563388 248 ------SLQGHIKAHAGEEPYPCKQ-CGKAFRYASSLQKHEKTHIAQKPYVCNN--CGKGFRCSSSLRDHERTHTGEKPY 318
Cdd:COG5048 368 nneppqSLQQYKDLKNDKKSETLSNsCIRNFKRDSNLSLHIITHLSFRPYNCKNppCSKSFNRHYNLIPHKKIHTNHAPL 447

                       170       180
                ....*....|....*....|
gi 68563388 319 ECQKCGKaFSRASTLWKHKK 338
Cdd:COG5048 448 LCSILKS-FRRDLDLSNHGK 466
zf-H2C2_2 pfam13465
Zinc-finger double domain;
192-217 1.06e-04

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 38.89  E-value: 1.06e-04
                          10        20
                  ....*....|....*....|....*.
gi 68563388   192 HLRDHERTHTGEKPYECKHCGKAFRY 217
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
 
Name Accession Description Interval E-value
KRAB pfam01352
KRAB box; The KRAB domain (or Kruppel-associated box) is present in about a third of zinc ...
 12-53 2.02e-25

KRAB box; The KRAB domain (or Kruppel-associated box) is present in about a third of zinc finger proteins containing C2H2 fingers. The KRAB domain is found to be involved in protein-protein interactions. The KRAB domain is generally encoded by two exons. The regions coded by the two exons are known as KRAB-A and KRAB-B. The A box plays an important role in repression by binding to corepressors, while the B box is thought to enhance this repression brought about by the A box. KRAB-containing proteins are thought to have critical functions in cell proliferation and differentiation, apoptosis and neoplastic transformation.


Pssm-ID: 460171  Cd Length: 42  Bit Score: 96.39  E-value: 2.02e-25
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 68563388    12 SVAFEDVAVNFTQEEWALLDPSQKNLYRDVMQETFRNLASIG 53
Cdd:pfam01352   1 SVTFEDVAVDFTQEEWALLDPAQRNLYRDVMLENYRNLVSLG 42
KRAB smart00349
krueppel associated box;
13-55 3.01e-23

krueppel associated box;


Pssm-ID: 214630 [Multi-domain]  Cd Length: 61  Bit Score: 91.11  E-value: 3.01e-23
                           10        20        30        40
                   ....*....|....*....|....*....|....*....|...
gi 68563388     13 VAFEDVAVNFTQEEWALLDPSQKNLYRDVMQETFRNLASIGNK 55
Cdd:smart00349   1 VTFEDVAVYFTQEEWEQLDPAQKNLYRDVMLENYSNLVSLGFQ 43
KRAB_A-box cd07765
KRAB (Kruppel-associated box) domain -A box; The KRAB domain is a transcription repression ...
 13-52 6.39e-21

KRAB (Kruppel-associated box) domain -A box; The KRAB domain is a transcription repression module, found in a subgroup of the zinc finger proteins (ZFPs) of the C2H2 family, KRAB-ZFPs. KRAB-ZFPs comprise the largest group of transcriptional regulators in mammals, and are only found in tetrapods. These proteins have been shown to play important roles in cell differentiation and organ development, and in regulating viral replication and transcription. A KRAB domain may consist of an A-box, or of an A-box plus either a B-box, a divergent B-box (b), or a C-box. Only the A-box is included in this model. The A-box is needed for repression, the B- and C- boxes are not. KRAB-ZFPs have one or two KRAB domains at their amino-terminal end, and multiple C2H2 zinc finger motifs at their C-termini. Some KRAB-ZFPs also contain a SCAN domain which mediates homo- and hetero-oligomerization. The KRAB domain is a protein-protein interaction module which represses transcription through recruiting corepressors. A key mechanism appears to be the following: KRAB-AFPs tethered to DNA recruit, via their KRAB domain, the repressor KAP1 (KRAB-associated protein-1, also known as transcription intermediary factor 1 beta , KRAB-A interacting protein , and tripartite motif protein 28). The KAP1/ KRAB-AFP complex in turn recruits the heterochromatin protein 1 (HP1) family, and other chromatin modulating proteins, leading to transcriptional repression through heterochromatin formation.


Pssm-ID: 143639  Cd Length: 40  Bit Score: 84.14  E-value: 6.39e-21
                        10        20        30        40
                ....*....|....*....|....*....|....*....|
gi 68563388  13 VAFEDVAVNFTQEEWALLDPSQKNLYRDVMQETFRNLASI 52
Cdd:cd07765   1 VTFEDVAVYFSQEEWELLDPAQRDLYRDVMLENYENLVSL 40
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
176-338 7.56e-07

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 50.46  E-value: 7.56e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 68563388 176 KRYECKQCGKAFSRSSHLRDHERT--HTGE--KPYECKH--CGKAFRYSNCLHYHERTHTGEKPYVCM--ECGKAFSCLS 247
Cdd:COG5048 288 LPIKSKQCNISFSRSSPLTRHLRSvnHSGEslKPFSCPYslCGKLFSRNDALKRHILLHTSISPAKEKllNSSSKFSPLL 367

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 68563388 248 ------SLQGHIKAHAGEEPYPCKQ-CGKAFRYASSLQKHEKTHIAQKPYVCNN--CGKGFRCSSSLRDHERTHTGEKPY 318
Cdd:COG5048 368 nneppqSLQQYKDLKNDKKSETLSNsCIRNFKRDSNLSLHIITHLSFRPYNCKNppCSKSFNRHYNLIPHKKIHTNHAPL 447

                       170       180
                ....*....|....*....|
gi 68563388 319 ECQKCGKaFSRASTLWKHKK 338
Cdd:COG5048 448 LCSILKS-FRRDLDLSNHGK 466
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
67-349 3.74e-05

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 45.46  E-value: 3.74e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 68563388  67 NSSRNLRHIISHSGNNPYGCEECGkkpCTCKQCQKTSLSVTRVHRDT-----------VMHTGNGHY---GCTICEKVFN 132
Cdd:COG5048  45 RLEHLTRHIRSHTGEKPSQCSYSG---CDKSFSRPLELSRHLRTHHNnpsdlnskslpLSNSKASSSslsSSSSNSNDNN 121

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 68563388 133 IPSSFQIHQRNHTGEKPYECMEC--------GKALGFSRSLNRHKRIHTGEKRYECKQCGKAFSRSSHLRdheRTHTGEK 204
Cdd:COG5048 122 LLSSHSLPPSSRDPQLPDLLSISnlrnnplpGNNSSSVNTPQSNSLHPPLPANSLSKDPSSNLSLLISSN---VSTSIPS 198

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 68563388 205 PYECKHCGKAFRYSNCLHYHERTHTgEKPYVCMECGKAFSCLSSLQGHIKAHAGEEPYPCKQCG------KAFRYASSLQ 278
Cdd:COG5048 199 SSENSPLSSSYSIPSSSSDQNLENS-SSSLPLTTNSQLSPKSLLSQSPSSLSSSDSSSSASESPrsslptASSQSSSPNE 277

                       250       260       270       280       290       300       310
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 68563388 279 KHEKTHIAQ-KPYVCNNCGKGFRCSSSLRDHERT--HTGE--KPYEC--QKCGKAFSRASTLWKHKKTHTGEKPYKCK 349
Cdd:COG5048 278 SDSSSEKGFsLPIKSKQCNISFSRSSPLTRHLRSvnHSGEslKPFSCpySLCGKLFSRNDALKRHILLHTSISPAKEK 355
zf-H2C2_2 pfam13465
Zinc-finger double domain;
192-217 1.06e-04

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 38.89  E-value: 1.06e-04
                          10        20
                  ....*....|....*....|....*.
gi 68563388   192 HLRDHERTHTGEKPYECKHCGKAFRY 217
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
zf-H2C2_2 pfam13465
Zinc-finger double domain;
164-189 1.65e-04

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 38.12  E-value: 1.65e-04
                          10        20
                  ....*....|....*....|....*.
gi 68563388   164 SLNRHKRIHTGEKRYECKQCGKAFSR 189
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
zf-H2C2_2 pfam13465
Zinc-finger double domain;
304-329 1.84e-04

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 38.12  E-value: 1.84e-04
                          10        20
                  ....*....|....*....|....*.
gi 68563388   304 SLRDHERTHTGEKPYECQKCGKAFSR 329
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
SFP1 COG5189
Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division ...
 202-254 1.15e-03

Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division and chromosome partitioning];


Pssm-ID: 227516 [Multi-domain]  Cd Length: 423  Bit Score: 40.47  E-value: 1.15e-03
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 68563388 202 GEKPYECK--HCGKAFRYSNCLHYH---------------ERTHTG----EKPYVCMECGKAFSCLSSLQGHIK 254
Cdd:COG5189 346 DGKPYKCPveGCNKKYKNQNGLKYHmlhghqnqklhenpsPEKMNIfsakDKPYRCEVCDKRYKNLNGLKYHRK 419
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
178-306 1.68e-03

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 40.06  E-value: 1.68e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 68563388 178 YECKQCGKAFSRSSHLRDHERTHTGEKPYECKHCGKAFRYSN--CLHYHERTHTGEKPYVC-MECGKAFSCLSSLQGHIK 254
Cdd:COG5048  34 DSCPNCTDSFSRLEHLTRHIRSHTGEKPSQCSYSGCDKSFSRplELSRHLRTHHNNPSDLNsKSLPLSNSKASSSSLSSS 113

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|....*
gi 68563388 255 AHAGEEPYPCKQCGKAFRYASSLQKHEKTH---IAQKPYVCNNCGKGFRCSSSLR 306
Cdd:COG5048 114 SSNSNDNNLLSSHSLPPSSRDPQLPDLLSIsnlRNNPLPGNNSSSVNTPQSNSLH 168
zf-H2C2_2 pfam13465
Zinc-finger double domain;
224-245 2.08e-03

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 35.04  E-value: 2.08e-03
                          10        20
                  ....*....|....*....|..
gi 68563388   224 HERTHTGEKPYVCMECGKAFSC 245
Cdd:pfam13465   5 HMRTHTGEKPYKCPECGKSFKS 26
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 178-200 3.97e-03

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 34.20  E-value: 3.97e-03
                          10        20
                  ....*....|....*....|...
gi 68563388   178 YECKQCGKAFSRSSHLRDHERTH 200
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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