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Conserved domains on  [gi|67462078|sp|Q99PM3|]
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RecName: Full=Transcription initiation factor IIA subunit 1; AltName: Full=General transcription factor IIA subunit 1; Contains: RecName: Full=Transcription initiation factor IIA alpha chain; AltName: Full=TFIIA p35 subunit; Contains: RecName: Full=Transcription initiation factor IIA beta chain; AltName: Full=TFIIA p19 subunit

Protein Classification

transcription initiation factor IIA subunit 1 family protein( domain architecture ID 12042495)

transcription initiation factor IIA (TFIIA) subunit 1 family protein similar to TFIIA, a component of the transcription machinery of RNA polymerase II that plays an important role in transcriptional activation

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TFIIA pfam03153
Transcription factor IIA, alpha/beta subunit; Transcription initiation factor IIA (TFIIA) is a ...
 13-378 6.32e-56

Transcription factor IIA, alpha/beta subunit; Transcription initiation factor IIA (TFIIA) is a heterotrimer, the three subunits being known as alpha, beta, and gamma, in order of molecular weight. The N and C-terminal domains of the gamma subunit are represented in pfam02268 and pfam02751, respectively. This family represents the precursor that yields both the alpha and beta subunits. The TFIIA heterotrimer is an essential general transcription initiation factor for the expression of genes transcribed by RNA polymerase II. Together with TFIID, TFIIA binds to the promoter region; this is the first step in the formation of a pre-initiation complex (PIC). Binding of the rest of the transcription machinery follows this step. After initiation, the PIC does not completely dissociate from the promoter. Some components, including TFIIA, remain attached and re-initiate a subsequent round of transcription.


:

Pssm-ID: 460829 [Multi-domain]  Cd Length: 344  Bit Score: 186.86  E-value: 6.32e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 67462078    13 LYRSVIEDVINDVRDIFLDDGVDEQVLMELKTLWENKLMQSRAVD---GFHSEEQQLLLQVQQQHQPQQQQHHHHHHQHQ 89
Cdd:pfam03153   1 VYRSVIEDVINASRVDFEEEGVDEQVLEELKQLWQSKLSQSKVAEfpwDPKPEPPHPPPFVIKQEPGVTLQPASGPQAQL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 67462078    90 QAQPQQTVPQQAQTQQvlIPASQQATAPQVIVPdskLLQHMNASSITSAAATAATLALPAGVTPVQQLLTNSGQLLQVVR 169
Cdd:pfam03153  81 YKNVQAQGAAQRAAQP--LQQQQGSAAQSSINA---LQAGASQQQQALALPQQQPQPQQQQQQQQQQQQQQQQQQQQQQQ 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 67462078   170 aangaQYILQPQQSVVLQQQVIPQMQPGGVQAPVIQQVLAPLPGGISPQ--TGVIIQP---QQILFTGNKTQVIPTTVAA 244
Cdd:pfam03153 156 -----QQQEQAQQAPQQGGLNNSQTDGADDALEDWEGVLAQRRAAGAPEelGRVEIDRmlrEQIAARAKQMGGGLMLPLK 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 67462078   245 PAPAQAPMPAAGQQQPQAQPAqqqaplVLQVDGTGDTSSEEDEdeeedydddeeedkekdgaEDGQVEEEPLNSE-DDVS 323
Cdd:pfam03153 231 EALKGKSRAKKSRKAPRATAE------IAQLDGAGDDSDDEES-------------------SNEDDDEDAINSDlDDPD 285

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 67462078   324 DEEGQELFDTEN----VVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW 378
Cdd:pfam03153 286 DDDDDDLEDTDNalghVMLCQYDKVQRVKNKWKCTLKDGVMTVNGKDYVFHKATGEFEW 344
 
Name Accession Description Interval E-value
TFIIA pfam03153
Transcription factor IIA, alpha/beta subunit; Transcription initiation factor IIA (TFIIA) is a ...
 13-378 6.32e-56

Transcription factor IIA, alpha/beta subunit; Transcription initiation factor IIA (TFIIA) is a heterotrimer, the three subunits being known as alpha, beta, and gamma, in order of molecular weight. The N and C-terminal domains of the gamma subunit are represented in pfam02268 and pfam02751, respectively. This family represents the precursor that yields both the alpha and beta subunits. The TFIIA heterotrimer is an essential general transcription initiation factor for the expression of genes transcribed by RNA polymerase II. Together with TFIID, TFIIA binds to the promoter region; this is the first step in the formation of a pre-initiation complex (PIC). Binding of the rest of the transcription machinery follows this step. After initiation, the PIC does not completely dissociate from the promoter. Some components, including TFIIA, remain attached and re-initiate a subsequent round of transcription.


Pssm-ID: 460829 [Multi-domain]  Cd Length: 344  Bit Score: 186.86  E-value: 6.32e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 67462078    13 LYRSVIEDVINDVRDIFLDDGVDEQVLMELKTLWENKLMQSRAVD---GFHSEEQQLLLQVQQQHQPQQQQHHHHHHQHQ 89
Cdd:pfam03153   1 VYRSVIEDVINASRVDFEEEGVDEQVLEELKQLWQSKLSQSKVAEfpwDPKPEPPHPPPFVIKQEPGVTLQPASGPQAQL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 67462078    90 QAQPQQTVPQQAQTQQvlIPASQQATAPQVIVPdskLLQHMNASSITSAAATAATLALPAGVTPVQQLLTNSGQLLQVVR 169
Cdd:pfam03153  81 YKNVQAQGAAQRAAQP--LQQQQGSAAQSSINA---LQAGASQQQQALALPQQQPQPQQQQQQQQQQQQQQQQQQQQQQQ 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 67462078   170 aangaQYILQPQQSVVLQQQVIPQMQPGGVQAPVIQQVLAPLPGGISPQ--TGVIIQP---QQILFTGNKTQVIPTTVAA 244
Cdd:pfam03153 156 -----QQQEQAQQAPQQGGLNNSQTDGADDALEDWEGVLAQRRAAGAPEelGRVEIDRmlrEQIAARAKQMGGGLMLPLK 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 67462078   245 PAPAQAPMPAAGQQQPQAQPAqqqaplVLQVDGTGDTSSEEDEdeeedydddeeedkekdgaEDGQVEEEPLNSE-DDVS 323
Cdd:pfam03153 231 EALKGKSRAKKSRKAPRATAE------IAQLDGAGDDSDDEES-------------------SNEDDDEDAINSDlDDPD 285

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 67462078   324 DEEGQELFDTEN----VVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW 378
Cdd:pfam03153 286 DDDDDDLEDTDNalghVMLCQYDKVQRVKNKWKCTLKDGVMTVNGKDYVFHKATGEFEW 344
TFIIA_alpha_beta_like cd07976
Precursor of TFIIA alpha and beta subunits and similar proteins; Transcription factor II A ...
 318-378 8.43e-30

Precursor of TFIIA alpha and beta subunits and similar proteins; Transcription factor II A (TFIIA) is one of the general transcription factors for RNA polymerase II. TFIIA increases the affinity of TATA-binding protein (TBP) for DNA in order to assemble the initiation complex. TFIIA also functions as an activator during development and differentiation, and is involved in transcription from TATA-less promoters. TFIIA is composed of more than one subunit in various organisms. Mammalian TFIIA large subunits (TFIIA alpha and beta) and the smaller subunit (TFIIA gamma) form a heterotrimer. TFIIA alpha and beta are encoded by a single gene (TFIIA_alpha_beta), its protein product is post-translationally processed and cleaved. TOA1 and TOA2 are the two subunits of Yeast TFIIA which correspond to Mammalian TFIIA_alpha_beta and TFIIA gamma, respectively. TOA1 and TOA2 form a heterodimeric protein complex. TFIIA_alpha_beta alone is sufficient for transcription in early embryogenesis, but the cleaved forms, TFIIA alpha and TFIIA beta, represent the vast majority of TFIIA in most differentiated cells. The exact functional differences between cleaved and uncleaved forms are not yet clear. This model also contains paralogs of the canonical TFIIA_alpha_beta, such as the human ALF, which may be involved in gametogenesis and early embryogenesis (and is also subject to proteolytic cleavage).


Pssm-ID: 199899 [Multi-domain]  Cd Length: 102  Bit Score: 110.31  E-value: 8.43e-30
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 67462078 318 SEDDVSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW 378
Cdd:cd07976  42 SQSGAASFPWDPKPETENIVLCQYDKVTRVKNKWKCTLKDGIMTLNGKDYVFQKATGEFEW 102
TOA1 COG5149
Transcription initiation factor IIA, large chain [Transcription];
306-378 3.32e-15

Transcription initiation factor IIA, large chain [Transcription];


Pssm-ID: 227478 [Multi-domain]  Cd Length: 293  Bit Score: 75.48  E-value: 3.32e-15
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 67462078 306 AEDGQVEEEPLNSEDDVSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW 378
Cdd:COG5149 220 AKDEGMFSDLDDSDVDSGDSEIEGTKGSTNCMLCLYDKVNMSKGKWKCTFKDGVVSINNIDYVFNKAQGELEW 292
 
Name Accession Description Interval E-value
TFIIA pfam03153
Transcription factor IIA, alpha/beta subunit; Transcription initiation factor IIA (TFIIA) is a ...
 13-378 6.32e-56

Transcription factor IIA, alpha/beta subunit; Transcription initiation factor IIA (TFIIA) is a heterotrimer, the three subunits being known as alpha, beta, and gamma, in order of molecular weight. The N and C-terminal domains of the gamma subunit are represented in pfam02268 and pfam02751, respectively. This family represents the precursor that yields both the alpha and beta subunits. The TFIIA heterotrimer is an essential general transcription initiation factor for the expression of genes transcribed by RNA polymerase II. Together with TFIID, TFIIA binds to the promoter region; this is the first step in the formation of a pre-initiation complex (PIC). Binding of the rest of the transcription machinery follows this step. After initiation, the PIC does not completely dissociate from the promoter. Some components, including TFIIA, remain attached and re-initiate a subsequent round of transcription.


Pssm-ID: 460829 [Multi-domain]  Cd Length: 344  Bit Score: 186.86  E-value: 6.32e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 67462078    13 LYRSVIEDVINDVRDIFLDDGVDEQVLMELKTLWENKLMQSRAVD---GFHSEEQQLLLQVQQQHQPQQQQHHHHHHQHQ 89
Cdd:pfam03153   1 VYRSVIEDVINASRVDFEEEGVDEQVLEELKQLWQSKLSQSKVAEfpwDPKPEPPHPPPFVIKQEPGVTLQPASGPQAQL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 67462078    90 QAQPQQTVPQQAQTQQvlIPASQQATAPQVIVPdskLLQHMNASSITSAAATAATLALPAGVTPVQQLLTNSGQLLQVVR 169
Cdd:pfam03153  81 YKNVQAQGAAQRAAQP--LQQQQGSAAQSSINA---LQAGASQQQQALALPQQQPQPQQQQQQQQQQQQQQQQQQQQQQQ 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 67462078   170 aangaQYILQPQQSVVLQQQVIPQMQPGGVQAPVIQQVLAPLPGGISPQ--TGVIIQP---QQILFTGNKTQVIPTTVAA 244
Cdd:pfam03153 156 -----QQQEQAQQAPQQGGLNNSQTDGADDALEDWEGVLAQRRAAGAPEelGRVEIDRmlrEQIAARAKQMGGGLMLPLK 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 67462078   245 PAPAQAPMPAAGQQQPQAQPAqqqaplVLQVDGTGDTSSEEDEdeeedydddeeedkekdgaEDGQVEEEPLNSE-DDVS 323
Cdd:pfam03153 231 EALKGKSRAKKSRKAPRATAE------IAQLDGAGDDSDDEES-------------------SNEDDDEDAINSDlDDPD 285

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 67462078   324 DEEGQELFDTEN----VVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW 378
Cdd:pfam03153 286 DDDDDDLEDTDNalghVMLCQYDKVQRVKNKWKCTLKDGVMTVNGKDYVFHKATGEFEW 344
TFIIA_alpha_beta_like cd07976
Precursor of TFIIA alpha and beta subunits and similar proteins; Transcription factor II A ...
 318-378 8.43e-30

Precursor of TFIIA alpha and beta subunits and similar proteins; Transcription factor II A (TFIIA) is one of the general transcription factors for RNA polymerase II. TFIIA increases the affinity of TATA-binding protein (TBP) for DNA in order to assemble the initiation complex. TFIIA also functions as an activator during development and differentiation, and is involved in transcription from TATA-less promoters. TFIIA is composed of more than one subunit in various organisms. Mammalian TFIIA large subunits (TFIIA alpha and beta) and the smaller subunit (TFIIA gamma) form a heterotrimer. TFIIA alpha and beta are encoded by a single gene (TFIIA_alpha_beta), its protein product is post-translationally processed and cleaved. TOA1 and TOA2 are the two subunits of Yeast TFIIA which correspond to Mammalian TFIIA_alpha_beta and TFIIA gamma, respectively. TOA1 and TOA2 form a heterodimeric protein complex. TFIIA_alpha_beta alone is sufficient for transcription in early embryogenesis, but the cleaved forms, TFIIA alpha and TFIIA beta, represent the vast majority of TFIIA in most differentiated cells. The exact functional differences between cleaved and uncleaved forms are not yet clear. This model also contains paralogs of the canonical TFIIA_alpha_beta, such as the human ALF, which may be involved in gametogenesis and early embryogenesis (and is also subject to proteolytic cleavage).


Pssm-ID: 199899 [Multi-domain]  Cd Length: 102  Bit Score: 110.31  E-value: 8.43e-30
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 67462078 318 SEDDVSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW 378
Cdd:cd07976  42 SQSGAASFPWDPKPETENIVLCQYDKVTRVKNKWKCTLKDGIMTLNGKDYVFQKATGEFEW 102
TFIIA_alpha_beta_like cd07976
Precursor of TFIIA alpha and beta subunits and similar proteins; Transcription factor II A ...
 10-60 1.44e-22

Precursor of TFIIA alpha and beta subunits and similar proteins; Transcription factor II A (TFIIA) is one of the general transcription factors for RNA polymerase II. TFIIA increases the affinity of TATA-binding protein (TBP) for DNA in order to assemble the initiation complex. TFIIA also functions as an activator during development and differentiation, and is involved in transcription from TATA-less promoters. TFIIA is composed of more than one subunit in various organisms. Mammalian TFIIA large subunits (TFIIA alpha and beta) and the smaller subunit (TFIIA gamma) form a heterotrimer. TFIIA alpha and beta are encoded by a single gene (TFIIA_alpha_beta), its protein product is post-translationally processed and cleaved. TOA1 and TOA2 are the two subunits of Yeast TFIIA which correspond to Mammalian TFIIA_alpha_beta and TFIIA gamma, respectively. TOA1 and TOA2 form a heterodimeric protein complex. TFIIA_alpha_beta alone is sufficient for transcription in early embryogenesis, but the cleaved forms, TFIIA alpha and TFIIA beta, represent the vast majority of TFIIA in most differentiated cells. The exact functional differences between cleaved and uncleaved forms are not yet clear. This model also contains paralogs of the canonical TFIIA_alpha_beta, such as the human ALF, which may be involved in gametogenesis and early embryogenesis (and is also subject to proteolytic cleavage).


Pssm-ID: 199899 [Multi-domain]  Cd Length: 102  Bit Score: 91.05  E-value: 1.44e-22
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|.
gi 67462078  10 VPKLYRSVIEDVINDVRDIFLDDGVDEQVLMELKTLWENKLMQSRAVDGFH 60
Cdd:cd07976   1 VSKVYESVIEDVINNVREDFEDEGVDESVLQELKQLWEEKLSQSGAASFPW 51
TOA1 COG5149
Transcription initiation factor IIA, large chain [Transcription];
306-378 3.32e-15

Transcription initiation factor IIA, large chain [Transcription];


Pssm-ID: 227478 [Multi-domain]  Cd Length: 293  Bit Score: 75.48  E-value: 3.32e-15
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 67462078 306 AEDGQVEEEPLNSEDDVSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW 378
Cdd:COG5149 220 AKDEGMFSDLDDSDVDSGDSEIEGTKGSTNCMLCLYDKVNMSKGKWKCTFKDGVVSINNIDYVFNKAQGELEW 292
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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