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Conserved domains on  [gi|645036012|ref|WP_025411705|]
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GNAT family N-acetyltransferase [Gemmatirosa kalamazoonensis]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 10456837)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  15581578|10940244|9175471|27367672|26818562
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PhnO super family cl42766
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 61-149 3.65e-12

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


The actual alignment was detected with superfamily member COG0454:

Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 62.76  E-value: 3.65e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 645036012  61 IEDAADGAMLWRGERGEIMAF-NIAHLSGAEGWMGPLAVRPEAQGGGLGKEIVRTGVEWLRARGARAIGLETMPRTMDNI 139
Cdd:COG0454   28 EGSLAGAEFIAVDDKGEPIGFaGLRRLDDKVLELKRLYVLPEYRGKGIGKALLEALLEWARERGCTALELDTLDGNPAAI 107

                         90
                 ....*....|
gi 645036012 140 GFYSSLGFVP 149
Cdd:COG0454  108 RFYERLGFKE 117
 
Name Accession Description Interval E-value
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 61-149 3.65e-12

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 62.76  E-value: 3.65e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 645036012  61 IEDAADGAMLWRGERGEIMAF-NIAHLSGAEGWMGPLAVRPEAQGGGLGKEIVRTGVEWLRARGARAIGLETMPRTMDNI 139
Cdd:COG0454   28 EGSLAGAEFIAVDDKGEPIGFaGLRRLDDKVLELKRLYVLPEYRGKGIGKALLEALLEWARERGCTALELDTLDGNPAAI 107

                         90
                 ....*....|
gi 645036012 140 GFYSSLGFVP 149
Cdd:COG0454  108 RFYERLGFKE 117
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 58-147 1.06e-09

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 55.22  E-value: 1.06e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 645036012   58 RYAIEDAADGAMLWRGERGEIMAF---NIAHLSGAEGWMGPLAVRPEAQGGGLGKEIVRTGVEWLRARGARAIGLETMPR 134
Cdd:pfam00583  24 LEDWDEDASEGFFVAEEDGELVGFaslSIIDDEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFLEVAAD 103

                          90
                  ....*....|...
gi 645036012  135 TMDNIGFYSSLGF 147
Cdd:pfam00583 104 NLAAIALYEKLGF 116
PRK10975 PRK10975
dTDP-4-amino-4,6-dideoxy-D-galactose acyltransferase;
18-125 1.10e-06

dTDP-4-amino-4,6-dideoxy-D-galactose acyltransferase;


Pssm-ID: 182877  Cd Length: 194  Bit Score: 48.39  E-value: 1.10e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 645036012  18 VVRVEDIPALNDVFSEAFTerYRRdgmvgVRVPALNP-------AVWryaIEDAADGA-----MLWRGERGEIMAF-NIA 84
Cdd:PRK10975  51 VATETDIPALRQLAAQAFA--QSR-----FRAPWYAPddsgrfyAQW---IENAVRGTfdhqcLLLRDASGQIQGFvTLR 120

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|.
gi 645036012  85 HLSGAEGWMGPLAVRPEAQGGGLGKEIVRTGVEWLRARGAR 125
Cdd:PRK10975 121 ELNDTDARIGLLAVFPGAQGRGIGARLMQAALNWCQARGLT 161
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 76-130 1.85e-05

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 41.88  E-value: 1.85e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 645036012  76 GEIMAF---NIAHLSGAEGWMGPLAVRPEAQGGGLGKEIVRTGVEWLRARGARAIGLE 130
Cdd:cd04301    8 GEIVGFaslSPDGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
 
Name Accession Description Interval E-value
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 61-149 3.65e-12

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 62.76  E-value: 3.65e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 645036012  61 IEDAADGAMLWRGERGEIMAF-NIAHLSGAEGWMGPLAVRPEAQGGGLGKEIVRTGVEWLRARGARAIGLETMPRTMDNI 139
Cdd:COG0454   28 EGSLAGAEFIAVDDKGEPIGFaGLRRLDDKVLELKRLYVLPEYRGKGIGKALLEALLEWARERGCTALELDTLDGNPAAI 107

                         90
                 ....*....|
gi 645036012 140 GFYSSLGFVP 149
Cdd:COG0454  108 RFYERLGFKE 117
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
22-149 5.37e-12

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 62.41  E-value: 5.37e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 645036012  22 EDIPALNDVFSEAFTERYRRDGMVGVRvpalnpavwryaiEDAADGAMLWRGERGEIMAFNIAHLSGAEG-----WMGPL 96
Cdd:COG3153    7 EDAEAIAALLRAAFGPGREAELVDRLR-------------EDPAAGLSLVAEDDGEIVGHVALSPVDIDGegpalLLGPL 73

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 645036012  97 AVRPEAQGGGLGKEIVRTGVEWLRARGARAIGLETMPrtmDNIGFYSSLGFVP 149
Cdd:COG3153   74 AVDPEYRGQGIGRALMRAALEAARERGARAVVLLGDP---SLLPFYERFGFRP 123
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 85-161 1.31e-10

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 57.36  E-value: 1.31e-10
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 645036012  85 HLSGAEGWMGPLAVRPEAQGGGLGKEIVRTGVEWLRARGARAIGLETMPRTMDNIGFYSSLGFVPGRLTVTLTVDAA 161
Cdd:COG0456    8 VDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGFEEVGERPNYYGDDA 84
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 58-147 1.06e-09

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 55.22  E-value: 1.06e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 645036012   58 RYAIEDAADGAMLWRGERGEIMAF---NIAHLSGAEGWMGPLAVRPEAQGGGLGKEIVRTGVEWLRARGARAIGLETMPR 134
Cdd:pfam00583  24 LEDWDEDASEGFFVAEEDGELVGFaslSIIDDEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFLEVAAD 103

                          90
                  ....*....|...
gi 645036012  135 TMDNIGFYSSLGF 147
Cdd:pfam00583 104 NLAAIALYEKLGF 116
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 76-149 3.14e-08

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 51.53  E-value: 3.14e-08
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 645036012  76 GEIMAF-NIAHLSGAEGWMGPLAVRPEAQGGGLGKEIVRTGVEWLRARGARAIGLETMPRTmdnIGFYSSLGFVP 149
Cdd:COG1246   37 GEIVGCaALHPLDEDLAELRSLAVHPDYRGRGIGRRLLEALLAEARELGLKRLFLLTTSAA---IHFYEKLGFEE 108
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
83-149 5.40e-08

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 50.95  E-value: 5.40e-08
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 645036012  83 IAHLSGAEGWMGPLAVRPEAQGGGLGKEIVRTGVEWLRARGARAIGLETmpRTmDNIGFYSSLGFVP 149
Cdd:COG2153   51 LLPPGDGEAKIGRVAVLPEYRGQGLGRALMEAAIEEARERGARRIVLSA--QA-HAVGFYEKLGFVP 114
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 74-149 1.54e-07

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 48.22  E-value: 1.54e-07
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 645036012   74 ERGEIMAFNIAHLSGAEGWMG--PLAVRPEAQGGGLGKEIVRTGVEWLRARGARAIGLETMPRTmdnIGFYSSLGFVP 149
Cdd:pfam13508  10 DDGKIVGFAALLPLDDEGALAelRLAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLELETTNRA---AAFYEKLGFEE 84
PRK10975 PRK10975
dTDP-4-amino-4,6-dideoxy-D-galactose acyltransferase;
18-125 1.10e-06

dTDP-4-amino-4,6-dideoxy-D-galactose acyltransferase;


Pssm-ID: 182877  Cd Length: 194  Bit Score: 48.39  E-value: 1.10e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 645036012  18 VVRVEDIPALNDVFSEAFTerYRRdgmvgVRVPALNP-------AVWryaIEDAADGA-----MLWRGERGEIMAF-NIA 84
Cdd:PRK10975  51 VATETDIPALRQLAAQAFA--QSR-----FRAPWYAPddsgrfyAQW---IENAVRGTfdhqcLLLRDASGQIQGFvTLR 120

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|.
gi 645036012  85 HLSGAEGWMGPLAVRPEAQGGGLGKEIVRTGVEWLRARGAR 125
Cdd:PRK10975 121 ELNDTDARIGLLAVFPGAQGRGIGARLMQAALNWCQARGLT 161
PRK03624 PRK03624
putative acetyltransferase; Provisional
 88-147 2.05e-06

putative acetyltransferase; Provisional


Pssm-ID: 235142 [Multi-domain]  Cd Length: 140  Bit Score: 46.46  E-value: 2.05e-06
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 645036012  88 GAEGWMGPLAVRPEAQGGGLGKEIVRTGVEWLRARGARAIGLETMPRTMDNIGFYSSLGF 147
Cdd:PRK03624  66 GHRGWAYYLAVHPDFRGRGIGRALVARLEKKLIARGCPKINLQVREDNDAVLGFYEALGY 125
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
83-149 2.55e-06

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 44.90  E-value: 2.55e-06
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 645036012  83 IAHLSGAEGWMGPLAVRPEAQGGGLGKEIVRTGVEWLRARGARAIGLETMPRTMDNIGFYSSLGFVP 149
Cdd:COG3393    8 VRAESPGVAEISGVYTHPEYRGRGLASALVAALAREALARGARTPFLYVDADNPAARRLYERLGFRP 74
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
20-149 7.16e-06

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 45.37  E-value: 7.16e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 645036012  20 RVEDIPALNDVFSEAFTERYRRDGMVGVRVPAlnpavWRYAIEDAADGAMLWR--GERGEIMAFniAHLSGAEGWMGP-- 95
Cdd:COG1247    8 TPEDAPAIAAIYNEAIAEGTATFETEPPSEEE-----REAWFAAILAPGRPVLvaEEDGEVVGF--ASLGPFRPRPAYrg 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 645036012  96 -----LAVRPEAQGGGLGKEIVRTGVEWLRARGARAIGLETMPRTMDNIGFYSSLGFVP 149
Cdd:COG1247   81 taeesIYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLADNEASIALYEKLGFEE 139
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 76-130 1.85e-05

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 41.88  E-value: 1.85e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 645036012  76 GEIMAF---NIAHLSGAEGWMGPLAVRPEAQGGGLGKEIVRTGVEWLRARGARAIGLE 130
Cdd:cd04301    8 GEIVGFaslSPDGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
rimI PRK09491
ribosomal-protein-alanine N-acetyltransferase; Provisional
 59-147 1.23e-03

ribosomal-protein-alanine N-acetyltransferase; Provisional


Pssm-ID: 181904 [Multi-domain]  Cd Length: 146  Bit Score: 38.76  E-value: 1.23e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 645036012  59 YAIEDAADgAMLWR--------GER---------GEIMAFNIAHLSGAEGWMGPLAVRPEAQGGGLGKEIVRTGVEWLRA 121
Cdd:PRK09491  16 YHIEQRAH-AFPWSektfasnqGERylnlkltvnGQMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEK 94

                         90       100
                 ....*....|....*....|....*.
gi 645036012 122 RGARAIGLETMPRTMDNIGFYSSLGF 147
Cdd:PRK09491  95 RGVATLWLEVRASNAAAIALYESLGF 120
PLN02706 PLN02706
glucosamine 6-phosphate N-acetyltransferase
 29-148 5.10e-03

glucosamine 6-phosphate N-acetyltransferase


Pssm-ID: 178308 [Multi-domain]  Cd Length: 150  Bit Score: 36.99  E-value: 5.10e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 645036012  29 DVFSEAFTERYRRdgmvgvrVPALNPAVWRYAIEDAADGAMLWRG----ERgeimafNIAHLSGAEGWMGPLAVRPEAQG 104
Cdd:PLN02706  33 DVTEEEFEARFQE-------LASLGDDHLICVIEDAASGRIIATGsvfvER------KFIRNCGKVGHIEDVVVDSAARG 99

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....
gi 645036012 105 GGLGKEIVRTGVEWLRARGARAIGLETMPrtmDNIGFYSSLGFV 148
Cdd:PLN02706 100 KGLGKKIIEALTEHARSAGCYKVILDCSE---ENKAFYEKCGYV 140
RimL COG1670
Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, ...
 19-149 5.90e-03

Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 441276 [Multi-domain]  Cd Length: 173  Bit Score: 36.90  E-value: 5.90e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 645036012  19 VRVEDIPALNDVFSEAFTERYRRDGMV----------GVRVPALNPAVWRYAIEDAADGAMLwrgerGEIMAFNIAHLSG 88
Cdd:COG1670   13 LRPEDAEALAELLNDPEVARYLPGPPYsleearawleRLLADWADGGALPFAIEDKEDGELI-----GVVGLYDIDRANR 87

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 645036012  89 aEGWMGpLAVRPEAQGGGLGKEIVRTGVEWL-RARGARAIGLETMPrtmDN---IGFYSSLGFVP 149
Cdd:COG1670   88 -SAEIG-YWLAPAYWGKGYATEALRALLDYAfEELGLHRVEAEVDP---DNtasIRVLEKLGFRL 147
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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