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Conserved domains on  [gi|6324364|ref|NP_014434|]
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putative mitochondrial 37S ribosomal protein MRPS12 [Saccharomyces cerevisiae S288C]

Protein Classification

30S ribosomal protein S12( domain architecture ID 10795611)

30S ribosomal protein S12 assists in rRNA folding and the binding of other proteins during small subunit assembly

Gene Ontology:  GO:0006412|GO:0019843|GO:0003735
PubMed:  24524803

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
rpsL_bact TIGR00981
ribosomal protein S12, bacterial/organelle; This model recognizes ribosomal protein S12 of ...
 28-152 4.18e-69

ribosomal protein S12, bacterial/organelle; This model recognizes ribosomal protein S12 of Bacteria, mitochondria, and chloroplasts. The homologous ribosomal proteins of Archaea and Eukarya, termed S23 in Eukarya and S12 or S23 in Archaea, score below the trusted cutoff. [Protein synthesis, Ribosomal proteins: synthesis and modification]


:

Pssm-ID: 130054  Cd Length: 124  Bit Score: 204.63  E-value: 4.18e-69
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324364     28 QATLNQIKRgSGPPRRKKISTAPQLDQCPQRKGVVLRVMVLKPKKPNSAQRKACRVRLTNGNVVSAYIPGEGHDAQEHSI 107
Cdd:TIGR00981   1 MPTINQLIR-KGRKKKKKKSKSPALEACPQKRGVCTRVYTTTPKKPNSALRKVARVRLTNGFEVTAYIPGEGHNLQEHSV 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 6324364    108 VYVRGGRCQDLPGVKYHVIRGAGDLSGVVNRISSRSKYGAKKPSK 152
Cdd:TIGR00981  80 VLIRGGRVKDLPGVRYHIVRGALDTAGVKNRKQGRSKYGAKRPKA 124
 
Name Accession Description Interval E-value
rpsL_bact TIGR00981
ribosomal protein S12, bacterial/organelle; This model recognizes ribosomal protein S12 of ...
 28-152 4.18e-69

ribosomal protein S12, bacterial/organelle; This model recognizes ribosomal protein S12 of Bacteria, mitochondria, and chloroplasts. The homologous ribosomal proteins of Archaea and Eukarya, termed S23 in Eukarya and S12 or S23 in Archaea, score below the trusted cutoff. [Protein synthesis, Ribosomal proteins: synthesis and modification]


Pssm-ID: 130054  Cd Length: 124  Bit Score: 204.63  E-value: 4.18e-69
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324364     28 QATLNQIKRgSGPPRRKKISTAPQLDQCPQRKGVVLRVMVLKPKKPNSAQRKACRVRLTNGNVVSAYIPGEGHDAQEHSI 107
Cdd:TIGR00981   1 MPTINQLIR-KGRKKKKKKSKSPALEACPQKRGVCTRVYTTTPKKPNSALRKVARVRLTNGFEVTAYIPGEGHNLQEHSV 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 6324364    108 VYVRGGRCQDLPGVKYHVIRGAGDLSGVVNRISSRSKYGAKKPSK 152
Cdd:TIGR00981  80 VLIRGGRVKDLPGVRYHIVRGALDTAGVKNRKQGRSKYGAKRPKA 124
RpsL COG0048
Ribosomal protein S12 [Translation, ribosomal structure and biogenesis]; Ribosomal protein S12 ...
 30-152 3.37e-61

Ribosomal protein S12 [Translation, ribosomal structure and biogenesis]; Ribosomal protein S12 is part of the Pathway/BioSystem: Ribosome 30S subunit


Pssm-ID: 439818  Cd Length: 124  Bit Score: 184.64  E-value: 3.37e-61
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324364   30 TLNQIKRGSGPPRRKKiSTAPQLDQCPQRKGVVLRVMVLKPKKPNSAQRKACRVRLTNGNVVSAYIPGEGHDAQEHSIVY 109
Cdd:COG0048   3 TINQLVRKGRKKKKKK-SKSPALKGCPQKRGVCTRVYTTTPKKPNSALRKVARVRLSNGIEVTAYIPGEGHNLQEHSVVL 81

                        90       100       110       120
                ....*....|....*....|....*....|....*....|...
gi 6324364  110 VRGGRCQDLPGVKYHVIRGAGDLSGVVNRISSRSKYGAKKPSK 152
Cdd:COG0048  82 VRGGRVKDLPGVRYHIVRGALDTAGVKGRKQSRSKYGAKKPKA 124
Ribosomal_S12 cd03368
S12-like family, 30S ribosomal protein S12 subfamily; S12 is located at the interface of the ...
 30-138 2.34e-59

S12-like family, 30S ribosomal protein S12 subfamily; S12 is located at the interface of the large and small ribosomal subunits of prokaryotes, chloroplasts and mitochondria, where it plays an important role in both tRNA and ribosomal subunit interactions. S12 is essential for maintenance of a pretranslocation state and, together with S13, functions as a control element for the rRNA- and tRNA-driven movements of translocation. Antibiotics such as streptomycin bind S12 and cause the ribosome to misread the genetic code.


Pssm-ID: 239466  Cd Length: 108  Bit Score: 179.25  E-value: 2.34e-59
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324364   30 TLNQIKRgSGPPRRKKISTAPQLDQCPQRKGVVLRVMVLKPKKPNSAQRKACRVRLTNGNVVSAYIPGEGHDAQEHSIVY 109
Cdd:cd03368   1 TINQLIR-KGRKKKKKKSKSPALEGCPQKKGVCLKVYTTTPKKPNSALRKVARVRLSNGKEVTAYIPGEGHNLQEHSVVL 79

                        90       100
                ....*....|....*....|....*....
gi 6324364  110 VRGGRCQDLPGVKYHVIRGAGDLSGVVNR 138
Cdd:cd03368  80 VRGGRVKDLPGVRYHIVRGVLDLAGVKNR 108
rps12 CHL00051
ribosomal protein S12
 30-151 3.06e-52

ribosomal protein S12


Pssm-ID: 176992  Cd Length: 123  Bit Score: 161.73  E-value: 3.06e-52
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324364    30 TLNQIKRGSGPPRRKKiSTAPQLDQCPQRKGVVLRVMVLKPKKPNSAQRKACRVRLTNGNVVSAYIPGEGHDAQEHSIVY 109
Cdd:CHL00051   3 TIKQLIRNTRQPIRNK-TKSPALRGCPQRRGTCTRVYTITPKKPNSALRKVARVRLTSGFEITAYIPGIGHNLQEHSVVL 81

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|..
gi 6324364   110 VRGGRCQDLPGVKYHVIRGAGDLSGVVNRISSRSKYGAKKPS 151
Cdd:CHL00051  82 VRGGRVKDLPGVRYHIVRGTLDAVGVKDRQQGRSKYGVKKPK 123
Ribosom_S12_S23 pfam00164
Ribosomal protein S12/S23; This protein is known as S12 in bacteria and archaea and S23 in ...
 42-150 4.49e-35

Ribosomal protein S12/S23; This protein is known as S12 in bacteria and archaea and S23 in eukaryotes.


Pssm-ID: 395112  Cd Length: 114  Bit Score: 117.94  E-value: 4.49e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324364     42 RRKKIST--APQLDQCPQRKGVVLRVMVLKPKKPNSAQRKACRVRLT-NGNVVSAYIPGEG--HDAQEHSIVYVRGGRCQ 116
Cdd:pfam00164   1 RRLLGLKtkSPPLEGAPQARGVVLEKVTVEPKQPNSALRKCVRVRLIkNGKEVTAFIPGEGaiHFLQEHDEVLIRGGGGP 80

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 6324364    117 -----DLPGVKYHVIRGAGdlsgvvNRISSRSKYGAKKP 150
Cdd:pfam00164  81 ghavgDLPGVRYKVVRVAG------VSLQALSKYGAEKP 113
 
Name Accession Description Interval E-value
rpsL_bact TIGR00981
ribosomal protein S12, bacterial/organelle; This model recognizes ribosomal protein S12 of ...
 28-152 4.18e-69

ribosomal protein S12, bacterial/organelle; This model recognizes ribosomal protein S12 of Bacteria, mitochondria, and chloroplasts. The homologous ribosomal proteins of Archaea and Eukarya, termed S23 in Eukarya and S12 or S23 in Archaea, score below the trusted cutoff. [Protein synthesis, Ribosomal proteins: synthesis and modification]


Pssm-ID: 130054  Cd Length: 124  Bit Score: 204.63  E-value: 4.18e-69
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324364     28 QATLNQIKRgSGPPRRKKISTAPQLDQCPQRKGVVLRVMVLKPKKPNSAQRKACRVRLTNGNVVSAYIPGEGHDAQEHSI 107
Cdd:TIGR00981   1 MPTINQLIR-KGRKKKKKKSKSPALEACPQKRGVCTRVYTTTPKKPNSALRKVARVRLTNGFEVTAYIPGEGHNLQEHSV 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 6324364    108 VYVRGGRCQDLPGVKYHVIRGAGDLSGVVNRISSRSKYGAKKPSK 152
Cdd:TIGR00981  80 VLIRGGRVKDLPGVRYHIVRGALDTAGVKNRKQGRSKYGAKRPKA 124
RpsL COG0048
Ribosomal protein S12 [Translation, ribosomal structure and biogenesis]; Ribosomal protein S12 ...
 30-152 3.37e-61

Ribosomal protein S12 [Translation, ribosomal structure and biogenesis]; Ribosomal protein S12 is part of the Pathway/BioSystem: Ribosome 30S subunit


Pssm-ID: 439818  Cd Length: 124  Bit Score: 184.64  E-value: 3.37e-61
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324364   30 TLNQIKRGSGPPRRKKiSTAPQLDQCPQRKGVVLRVMVLKPKKPNSAQRKACRVRLTNGNVVSAYIPGEGHDAQEHSIVY 109
Cdd:COG0048   3 TINQLVRKGRKKKKKK-SKSPALKGCPQKRGVCTRVYTTTPKKPNSALRKVARVRLSNGIEVTAYIPGEGHNLQEHSVVL 81

                        90       100       110       120
                ....*....|....*....|....*....|....*....|...
gi 6324364  110 VRGGRCQDLPGVKYHVIRGAGDLSGVVNRISSRSKYGAKKPSK 152
Cdd:COG0048  82 VRGGRVKDLPGVRYHIVRGALDTAGVKGRKQSRSKYGAKKPKA 124
Ribosomal_S12 cd03368
S12-like family, 30S ribosomal protein S12 subfamily; S12 is located at the interface of the ...
 30-138 2.34e-59

S12-like family, 30S ribosomal protein S12 subfamily; S12 is located at the interface of the large and small ribosomal subunits of prokaryotes, chloroplasts and mitochondria, where it plays an important role in both tRNA and ribosomal subunit interactions. S12 is essential for maintenance of a pretranslocation state and, together with S13, functions as a control element for the rRNA- and tRNA-driven movements of translocation. Antibiotics such as streptomycin bind S12 and cause the ribosome to misread the genetic code.


Pssm-ID: 239466  Cd Length: 108  Bit Score: 179.25  E-value: 2.34e-59
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324364   30 TLNQIKRgSGPPRRKKISTAPQLDQCPQRKGVVLRVMVLKPKKPNSAQRKACRVRLTNGNVVSAYIPGEGHDAQEHSIVY 109
Cdd:cd03368   1 TINQLIR-KGRKKKKKKSKSPALEGCPQKKGVCLKVYTTTPKKPNSALRKVARVRLSNGKEVTAYIPGEGHNLQEHSVVL 79

                        90       100
                ....*....|....*....|....*....
gi 6324364  110 VRGGRCQDLPGVKYHVIRGAGDLSGVVNR 138
Cdd:cd03368  80 VRGGRVKDLPGVRYHIVRGVLDLAGVKNR 108
rps12 CHL00051
ribosomal protein S12
 30-151 3.06e-52

ribosomal protein S12


Pssm-ID: 176992  Cd Length: 123  Bit Score: 161.73  E-value: 3.06e-52
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324364    30 TLNQIKRGSGPPRRKKiSTAPQLDQCPQRKGVVLRVMVLKPKKPNSAQRKACRVRLTNGNVVSAYIPGEGHDAQEHSIVY 109
Cdd:CHL00051   3 TIKQLIRNTRQPIRNK-TKSPALRGCPQRRGTCTRVYTITPKKPNSALRKVARVRLTSGFEITAYIPGIGHNLQEHSVVL 81

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|..
gi 6324364   110 VRGGRCQDLPGVKYHVIRGAGDLSGVVNRISSRSKYGAKKPS 151
Cdd:CHL00051  82 VRGGRVKDLPGVRYHIVRGTLDAVGVKDRQQGRSKYGVKKPK 123
PTZ00115 PTZ00115
40S ribosomal protein S12; Provisional
 33-148 1.19e-38

40S ribosomal protein S12; Provisional


Pssm-ID: 240276  Cd Length: 290  Bit Score: 132.27  E-value: 1.19e-38
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324364    33 QIKRGSGPPRRKKistapQLDQCPQRKGVVLRVMVLKPKKPNSAQRKACRVRLTNGNVVSAYIPGEGHDAQEHSIVYVRG 112
Cdd:PTZ00115 106 QVPKLKPKNRRSK-----WLEGAPQKKGICVKVRVQTPRKPNSGLRKVARVRLSTGRTVTVYIPGIGHNLNTHSVVLVRG 180

                         90       100       110
                 ....*....|....*....|....*....|....*.
gi 6324364   113 GRCQDLPGVKYHVIRGAGDLSGVVNRISSRSKYGAK 148
Cdd:PTZ00115 181 GRCKDVPGCNYKAVRGVYDLLPVKNRARSRSKYGVK 216
Ribosom_S12_S23 pfam00164
Ribosomal protein S12/S23; This protein is known as S12 in bacteria and archaea and S23 in ...
 42-150 4.49e-35

Ribosomal protein S12/S23; This protein is known as S12 in bacteria and archaea and S23 in eukaryotes.


Pssm-ID: 395112  Cd Length: 114  Bit Score: 117.94  E-value: 4.49e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324364     42 RRKKIST--APQLDQCPQRKGVVLRVMVLKPKKPNSAQRKACRVRLT-NGNVVSAYIPGEG--HDAQEHSIVYVRGGRCQ 116
Cdd:pfam00164   1 RRLLGLKtkSPPLEGAPQARGVVLEKVTVEPKQPNSALRKCVRVRLIkNGKEVTAFIPGEGaiHFLQEHDEVLIRGGGGP 80

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 6324364    117 -----DLPGVKYHVIRGAGdlsgvvNRISSRSKYGAKKP 150
Cdd:pfam00164  81 ghavgDLPGVRYKVVRVAG------VSLQALSKYGAEKP 113
Ribosomal_S12_like cd00319
Ribosomal protein S12-like family; composed of prokaryotic 30S ribosomal protein S12, ...
 59-133 3.99e-16

Ribosomal protein S12-like family; composed of prokaryotic 30S ribosomal protein S12, eukaryotic 40S ribosomal protein S23 and similar proteins. S12 and S23 are located at the interface of the large and small ribosomal subunits, adjacent to the decoding center. They play an important role in translocation during the peptide elongation step of protein synthesis. They are also involved in important RNA and protein interactions. Ribosomal protein S12 is essential for maintenance of a pretranslocation state and, together with S13, functions as a control element for the rRNA- and tRNA-driven movements of translocation. S23 interacts with domain III of the eukaryotic elongation factor 2 (eEF2), which catalyzes translocation. Mutations in S12 and S23 have been found to affect translational accuracy. Antibiotics such as streptomycin may also bind S12/S23 and cause the ribosome to misread the genetic code.


Pssm-ID: 238196  Cd Length: 95  Bit Score: 68.97  E-value: 3.99e-16
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 6324364   59 KGVVLRVMVLKPKKPNSAQRKACRVRLTNGNVVSAYIPGEGHDAQEHSIVYVRGGRCQDLPGVKYHVIRGAGDLS 133
Cdd:cd00319  16 RGVCTVVRTVTPKKPNSALRKVAKVRLTSGYEVTAYIPGEGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGVYDAA 90
Ribosomal_S23 cd03367
S12-like family, 40S ribosomal protein S23 subfamily; S23 is located at the interface of the ...
 49-149 1.02e-12

S12-like family, 40S ribosomal protein S23 subfamily; S23 is located at the interface of the large and small ribosomal subunits of eukaryotes, adjacent to the decoding center. It interacts with domain III of the eukaryotic elongation factor 2 (eEF2), which catalyzes the translocation of the growing peptidyl-tRNA to the P site to make room for the next aminoacyl-tRNA at the A (acceptor) site. Through its interaction with eEF2, S23 may play an important role in translocation. Also members of this subfamily are the archaeal 30S ribosomal S12 proteins. Prokaryotic S12 is essential for maintenance of a pretranslocation state and, together with S13, functions as control element for the rRNA- and tRNA-driven movements of translocation. S12 and S23 are also implicated in translation accuracy. Antibiotics such as streptomycin bind S12/S23 and cause the ribosome to misread the genetic code.


Pssm-ID: 239465  Cd Length: 115  Bit Score: 60.75  E-value: 1.02e-12
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324364   49 APQLDQCPQRKGVVLRVMVLKPKKPNSAQRKACRVRLT-NGNVVSAYIPGEG--HDAQEHSIVYV----RGGRCQ-DLPG 120
Cdd:cd03367  13 ADPLGGAPHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIkNGKKITAFVPGDGclNFIDENDEVLVagfgRKGRAVgDIPG 92

                        90       100
                ....*....|....*....|....*....
gi 6324364  121 VKYHVIRgagdlsgvVNRISSRSKYGAKK 149
Cdd:cd03367  93 VRFKVVK--------VNGVSLLALFKGKK 113
rps12P PRK04211
30S ribosomal protein S12P; Reviewed
 52-149 5.93e-12

30S ribosomal protein S12P; Reviewed


Pssm-ID: 235257  Cd Length: 145  Bit Score: 59.58  E-value: 5.93e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324364    52 LDQCPQRKGVVLRVMVLKPKKPNSAQRKACRVRLT-NGNVVSAYIPGEG--HDAQEHSIVYV------RGGRCQDLPGVK 122
Cdd:PRK04211  42 LEGAPMARGIVLEKVGVEAKQPNSAIRKCVRVQLIkNGKQVTAFCPGDGaiNFIDEHDEVVIegiggpKGRSMGDIPGVR 121

                         90       100
                 ....*....|....*....|....*..
gi 6324364   123 YHVIRgagdlsgvVNRISSRSKYGAKK 149
Cdd:PRK04211 122 YKVIK--------VNGVSLKELVKGKK 140
PTZ00067 PTZ00067
40S ribosomal S23; Provisional
 59-130 1.13e-08

40S ribosomal S23; Provisional


Pssm-ID: 185422  Cd Length: 143  Bit Score: 50.90  E-value: 1.13e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324364    59 KGVVLRVMVLKPKKPNSAQRKACRVRLT-NGNVVSAYIPGEG--HDAQEHSIVYVRG-GR----CQDLPGVKYHVIRGAG 130
Cdd:PTZ00067  48 KGIVVEKIGIEAKQPNSAIRKCVRVQLIkNGKKITAFVPNDGclNFINENDEVLVSGfGRsghaVGDIPGVRFKVVKVAG 127
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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