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Conserved domains on  [gi|609234|emb|CAA86583|]
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bluB [Rhodobacter capsulatus]

Protein Classification

5,6-dimethylbenzimidazole synthase( domain architecture ID 10114863)

5,6-dimethylbenzimidazole synthase (BluB) catalyzes the oxidative fragmentation and contraction of the isoalloxazine heterocycle and the cleavage of the ribityl tail of FMNH(2) to form 5,6-dimethylbenzimidazole (DMB) and D-erythrose 4-phosphate (E4P)

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
BluB cd02145
5,6-dimethylbenzimidazole synthase; BluB catalyzes the O2-dependent conversion of FMNH2 to 5, ...
 12-206 4.54e-94

5,6-dimethylbenzimidazole synthase; BluB catalyzes the O2-dependent conversion of FMNH2 to 5,6-dimethylbenzimidazole (DMB), a component of vitamin B12; is is a subfamily of the nitroreductase family; nitroreductases typically reduce their substrates by using NAD(P)H as electron donor and often use FMN as a cofactor.


:

Pssm-ID: 380321  Cd Length: 196  Bit Score: 272.69  E-value: 4.54e-94
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234    12 TEVLRWRRDVRHFRPDPIDEAVIDRLRAVMDMAPSVGNARPWRVIRVDSPALRAEVLANFNAARAAAGSAYAGEQAEAYA 91
Cdd:cd02145   1 YRVIRWRRDVRHFRPDPVPEEVLERLLQAAHLAPSVGLMQPWRFVRVRSAATRKAVHELFQRANAEAAEMYTGERAAQYR 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234    92 TLKLQGIDQAPLQLAVFTHRDPAAGHGLGRASMPVTLQQSTAMA-FTRSGCRAGENLGLGMVSVLDPKAVERLLNAPPDW 170
Cdd:cd02145  81 TLKLEGIEEAPLQLAVFCDRARAGGHGLGRTTMPEMDLYSSVCAvQNLWLAARAEGLGVGWVSILDPDEVKRLLGIPEHW 160

                       170       180       190
                ....*....|....*....|....*....|....*.
gi 609234   171 DFVAWLCIGVPEFTDDTPLLHRAGWQENLPTEWERR 206
Cdd:cd02145 161 EPVAYLCIGYPEFFYDEPELEQAGWEQRRPLEWVVF 196
 
Name Accession Description Interval E-value
BluB cd02145
5,6-dimethylbenzimidazole synthase; BluB catalyzes the O2-dependent conversion of FMNH2 to 5, ...
 12-206 4.54e-94

5,6-dimethylbenzimidazole synthase; BluB catalyzes the O2-dependent conversion of FMNH2 to 5,6-dimethylbenzimidazole (DMB), a component of vitamin B12; is is a subfamily of the nitroreductase family; nitroreductases typically reduce their substrates by using NAD(P)H as electron donor and often use FMN as a cofactor.


Pssm-ID: 380321  Cd Length: 196  Bit Score: 272.69  E-value: 4.54e-94
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234    12 TEVLRWRRDVRHFRPDPIDEAVIDRLRAVMDMAPSVGNARPWRVIRVDSPALRAEVLANFNAARAAAGSAYAGEQAEAYA 91
Cdd:cd02145   1 YRVIRWRRDVRHFRPDPVPEEVLERLLQAAHLAPSVGLMQPWRFVRVRSAATRKAVHELFQRANAEAAEMYTGERAAQYR 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234    92 TLKLQGIDQAPLQLAVFTHRDPAAGHGLGRASMPVTLQQSTAMA-FTRSGCRAGENLGLGMVSVLDPKAVERLLNAPPDW 170
Cdd:cd02145  81 TLKLEGIEEAPLQLAVFCDRARAGGHGLGRTTMPEMDLYSSVCAvQNLWLAARAEGLGVGWVSILDPDEVKRLLGIPEHW 160

                       170       180       190
                ....*....|....*....|....*....|....*.
gi 609234   171 DFVAWLCIGVPEFTDDTPLLHRAGWQENLPTEWERR 206
Cdd:cd02145 161 EPVAYLCIGYPEFFYDEPELEQAGWEQRRPLEWVVF 196
BluB TIGR02476
5,6-dimethylbenzimidazole synthase; A previously published hypothesis that BluB, involved in ...
 3-205 1.95e-71

5,6-dimethylbenzimidazole synthase; A previously published hypothesis that BluB, involved in cobalamin biosynthesis, is EC 1.16.8.1 (cob(II)yrinic acid a,c-diamide reductase) is now contradicted by newer work ascribing a role in 5,6-dimethylbenzimidazole (DMB) biosynthesis. The BluB protein is related to the nitroreductase family (pfam0881). [Biosynthesis of cofactors, prosthetic groups, and carriers, Heme, porphyrin, and cobalamin]


Pssm-ID: 162875  Cd Length: 205  Bit Score: 215.77  E-value: 1.95e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234       3 FEQTHRDALTEVLRWRRDVRHFRPDPIDEAVIDRLRAVMDMAPSVGNARPWRVIRVDSPALRAEVLANFNAARAAAGSAY 82
Cdd:TIGR02476   1 FSDAERAAVYRLIRERRDVRHFRSDPVPEAVLERLLDAAHHAPSVGFSQPWRFVRVESPATREAVHALFTRANQAAAAIY 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234      83 AGEQAEAYATLKLQGIDQAPLQLAVFTHRDPAAGHGLGRASMPVTLQQSTAMAFTRSGCRA-GENLGLGMVSVLDPKAVE 161
Cdd:TIGR02476  81 DGERASQYHRLKLEGIREAPVQLAVFCDDARGEGHGLGRHTMPEMLRYSVACAIQNLWLAArAEGLGVGWVSILDPDAVR 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....
gi 609234     162 RLLNAPPDWDFVAWLCIGVPEFTDDTPLLHRAGWQENLPTEWER 205
Cdd:TIGR02476 161 RLLGVPEGWRLVAYLCLGWPDAFYDEPELERAGWQERRPLEWRR 204
NfnB COG0778
Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway ...
 11-182 1.43e-25

Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440541 [Multi-domain]  Cd Length: 163  Bit Score: 96.85  E-value: 1.43e-25
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234    11 LTEVLRWRRDVRHFRPDPIDEAVIDRLRAVMDMAPSVGNARPWRVIRVDSPALRAEVlanfnaaraaagsayageqAEAY 90
Cdd:COG0778   1 LLELLLTRRSVRKFTDKPVSDEELEELLEAARLAPSAGNLQPWRFVVVRDPELRERL-------------------AEAL 61

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234    91 ATLKLQGIDQAPLQLAVFTHRDPAAGHglgrasMPVTLQQSTAMAFTrSGCRAGENLGLG--MVSVLDPKAVERLLNAPP 168
Cdd:COG0778  62 AEANQEWVADAPVLIVVCADPDRSEKV------PERYALLDAGIAAQ-NLLLAARALGLGtcWIGGFDPEKVRELLGLPE 134

                       170
                ....*....|....
gi 609234   169 DWDFVAWLCIGVPE 182
Cdd:COG0778 135 GEEPVALLALGYPA 148
Nitroreductase pfam00881
Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent ...
 15-179 1.68e-22

Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent and NAD(P)H-dependent enzymes able to metabolize nitrosubstituted compounds.


Pssm-ID: 425926 [Multi-domain]  Cd Length: 168  Bit Score: 89.37  E-value: 1.68e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234      15 LRWRRDVRHFRPDPIDEAVIDRLRAVMDMAPSVGNARPWRVIRVDSPALR---AEVLANFNAARAAAGSAYAGEQAEAYA 91
Cdd:pfam00881   1 IRQRRSVRKFDPEPVPKEVLEEILEAARRAPSAGNLQPWRFYVVTDGELRyrlAEAALELLLVEPAAALLLLLRRDANLK 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234      92 TLKLQGIDQAPLQLAVftHRDPAAGHGLGRASMPVTLQQSTAMAFTRS--GCRAgENLGLGMVSVLDPKAVERLLNAPPD 169
Cdd:pfam00881  81 LLLQDFLRGAPVLIVI--TASLSTYLRKAAERAYREALLDAGAAAQNLllAATS-LGLGSCPIGGFDAAAVRELLGLPDD 157

                         170
                  ....*....|
gi 609234     170 WDFVAWLCIG 179
Cdd:pfam00881 158 ERLVGLIAVG 167
PRK13294 PRK13294
F420-0--gamma-glutamyl ligase; Provisional
 4-68 3.96e-05

F420-0--gamma-glutamyl ligase; Provisional


Pssm-ID: 183957 [Multi-domain]  Cd Length: 448  Bit Score: 43.46  E-value: 3.96e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 609234      4 EQTHRDALTEvlrwRRDVRHFRPDPIDEAVIDRLRAVMDMAPSVGNARPWRVIRVDSPALRAEVL 68
Cdd:PRK13294 250 ELGRREAVLL----RRSVREFSDDPVDPEAVRRAVAAALTAPAPHHTRPVRFVWLRSAAVRTRLL 310
 
Name Accession Description Interval E-value
BluB cd02145
5,6-dimethylbenzimidazole synthase; BluB catalyzes the O2-dependent conversion of FMNH2 to 5, ...
 12-206 4.54e-94

5,6-dimethylbenzimidazole synthase; BluB catalyzes the O2-dependent conversion of FMNH2 to 5,6-dimethylbenzimidazole (DMB), a component of vitamin B12; is is a subfamily of the nitroreductase family; nitroreductases typically reduce their substrates by using NAD(P)H as electron donor and often use FMN as a cofactor.


Pssm-ID: 380321  Cd Length: 196  Bit Score: 272.69  E-value: 4.54e-94
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234    12 TEVLRWRRDVRHFRPDPIDEAVIDRLRAVMDMAPSVGNARPWRVIRVDSPALRAEVLANFNAARAAAGSAYAGEQAEAYA 91
Cdd:cd02145   1 YRVIRWRRDVRHFRPDPVPEEVLERLLQAAHLAPSVGLMQPWRFVRVRSAATRKAVHELFQRANAEAAEMYTGERAAQYR 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234    92 TLKLQGIDQAPLQLAVFTHRDPAAGHGLGRASMPVTLQQSTAMA-FTRSGCRAGENLGLGMVSVLDPKAVERLLNAPPDW 170
Cdd:cd02145  81 TLKLEGIEEAPLQLAVFCDRARAGGHGLGRTTMPEMDLYSSVCAvQNLWLAARAEGLGVGWVSILDPDEVKRLLGIPEHW 160

                       170       180       190
                ....*....|....*....|....*....|....*.
gi 609234   171 DFVAWLCIGVPEFTDDTPLLHRAGWQENLPTEWERR 206
Cdd:cd02145 161 EPVAYLCIGYPEFFYDEPELEQAGWEQRRPLEWVVF 196
BluB TIGR02476
5,6-dimethylbenzimidazole synthase; A previously published hypothesis that BluB, involved in ...
 3-205 1.95e-71

5,6-dimethylbenzimidazole synthase; A previously published hypothesis that BluB, involved in cobalamin biosynthesis, is EC 1.16.8.1 (cob(II)yrinic acid a,c-diamide reductase) is now contradicted by newer work ascribing a role in 5,6-dimethylbenzimidazole (DMB) biosynthesis. The BluB protein is related to the nitroreductase family (pfam0881). [Biosynthesis of cofactors, prosthetic groups, and carriers, Heme, porphyrin, and cobalamin]


Pssm-ID: 162875  Cd Length: 205  Bit Score: 215.77  E-value: 1.95e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234       3 FEQTHRDALTEVLRWRRDVRHFRPDPIDEAVIDRLRAVMDMAPSVGNARPWRVIRVDSPALRAEVLANFNAARAAAGSAY 82
Cdd:TIGR02476   1 FSDAERAAVYRLIRERRDVRHFRSDPVPEAVLERLLDAAHHAPSVGFSQPWRFVRVESPATREAVHALFTRANQAAAAIY 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234      83 AGEQAEAYATLKLQGIDQAPLQLAVFTHRDPAAGHGLGRASMPVTLQQSTAMAFTRSGCRA-GENLGLGMVSVLDPKAVE 161
Cdd:TIGR02476  81 DGERASQYHRLKLEGIREAPVQLAVFCDDARGEGHGLGRHTMPEMLRYSVACAIQNLWLAArAEGLGVGWVSILDPDAVR 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....
gi 609234     162 RLLNAPPDWDFVAWLCIGVPEFTDDTPLLHRAGWQENLPTEWER 205
Cdd:TIGR02476 161 RLLGVPEGWRLVAYLCLGWPDAFYDEPELERAGWQERRPLEWRR 204
NfnB COG0778
Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway ...
 11-182 1.43e-25

Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440541 [Multi-domain]  Cd Length: 163  Bit Score: 96.85  E-value: 1.43e-25
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234    11 LTEVLRWRRDVRHFRPDPIDEAVIDRLRAVMDMAPSVGNARPWRVIRVDSPALRAEVlanfnaaraaagsayageqAEAY 90
Cdd:COG0778   1 LLELLLTRRSVRKFTDKPVSDEELEELLEAARLAPSAGNLQPWRFVVVRDPELRERL-------------------AEAL 61

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234    91 ATLKLQGIDQAPLQLAVFTHRDPAAGHglgrasMPVTLQQSTAMAFTrSGCRAGENLGLG--MVSVLDPKAVERLLNAPP 168
Cdd:COG0778  62 AEANQEWVADAPVLIVVCADPDRSEKV------PERYALLDAGIAAQ-NLLLAARALGLGtcWIGGFDPEKVRELLGLPE 134

                       170
                ....*....|....
gi 609234   169 DWDFVAWLCIGVPE 182
Cdd:COG0778 135 GEEPVALLALGYPA 148
Nitroreductase pfam00881
Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent ...
 15-179 1.68e-22

Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent and NAD(P)H-dependent enzymes able to metabolize nitrosubstituted compounds.


Pssm-ID: 425926 [Multi-domain]  Cd Length: 168  Bit Score: 89.37  E-value: 1.68e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234      15 LRWRRDVRHFRPDPIDEAVIDRLRAVMDMAPSVGNARPWRVIRVDSPALR---AEVLANFNAARAAAGSAYAGEQAEAYA 91
Cdd:pfam00881   1 IRQRRSVRKFDPEPVPKEVLEEILEAARRAPSAGNLQPWRFYVVTDGELRyrlAEAALELLLVEPAAALLLLLRRDANLK 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234      92 TLKLQGIDQAPLQLAVftHRDPAAGHGLGRASMPVTLQQSTAMAFTRS--GCRAgENLGLGMVSVLDPKAVERLLNAPPD 169
Cdd:pfam00881  81 LLLQDFLRGAPVLIVI--TASLSTYLRKAAERAYREALLDAGAAAQNLllAATS-LGLGSCPIGGFDAAAVRELLGLPDD 157

                         170
                  ....*....|
gi 609234     170 WDFVAWLCIG 179
Cdd:pfam00881 158 ERLVGLIAVG 167
Nitro_FMN_reductase cd02062
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 18-179 4.12e-17

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380311 [Multi-domain]  Cd Length: 139  Bit Score: 74.26  E-value: 4.12e-17
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234    18 RRDVRHFRPDPIDEAVIDRLRAVMDMAPSVGNARPWRVIRVDSPALRaevlanfnaaraaagsayagEQAEAYATLKLQG 97
Cdd:cd02062   4 RRSIRKFTDKPVPEEKLRKILEAARLAPSAGNLQPWRFIVVRDREKK--------------------EKLAKLAAPNQKF 63

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234    98 IDQAPLQLAVFTHRDPAAGHGLGRASMPVTLQQSTAMAftrsgcragENLGLGMVSVLD--PKAVERLLNAPPDWDFVAW 175
Cdd:cd02062  64 IAGAPVVIVVVADPDKSRPWALEDAGAAAQNLLLAAAA---------LGLGSCWIGGFDfrEDKVRELLGIPENLRPVAL 134


                ....
gi 609234   176 LCIG 179
Cdd:cd02062 135 IAIG 138
nitroreductase cd02139
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 13-182 1.47e-11

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380316 [Multi-domain]  Cd Length: 165  Bit Score: 60.18  E-value: 1.47e-11
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234    13 EVLRWRRDVRHFRPDPIDEAVIDR-LRAVMdMAPSVGNARPWRVIRVDSPALRAEVlanfnaaraaagsayageqAEAYA 91
Cdd:cd02139   3 EAIKKRRSIRKYKPTPVEEEKLLRiLEAAR-LAPSAKNRQPWRFIVVKDKELKEKL-------------------AEAAN 62

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234    92 TLKlqGIDQAPLQLAVFTHRDpAAGHGLGRASMPVTLqqSTAMAFTrsgCRAGENLGLGM--VSVLDPKAVERLLNAPPD 169
Cdd:cd02139  63 GQK--FIAEAPVVIVACADPS-ESGMGCGKPYYLVDV--AIAMEHL---VLAATEEGLGTcwIGAFDEDKVKEILGIPEE 134

                       170
                ....*....|...
gi 609234   170 WDFVAWLCIGVPE 182
Cdd:cd02139 135 YRVVALTPLGYPA 147
YdjA-like cd02135
nitroreductase family protein similar to Escherichia coli YdjA; A subfamily of the ...
 13-179 1.85e-10

nitroreductase family protein similar to Escherichia coli YdjA; A subfamily of the nitroreductase family containing uncharacterized proteins that are similar to nitroreductase YdjA from Escherichia coli. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer. Members of this family are also called NADH dehydrogenase, oxygen-insensitive NAD(P)H nitrogenase or dihydropteridine reductase.


Pssm-ID: 380312 [Multi-domain]  Cd Length: 162  Bit Score: 57.23  E-value: 1.85e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234    13 EVLRWRRDVRHFRPD-PIDEAVIDR-LRAVMdMAPSVGNARPWRVIRVDSPALR--AEVLANFNAARAAAGSAYAGEQAE 88
Cdd:cd02135   2 ELIKTRRSIRKFKLTgAPPEEQLEElLEAAM-WAPNHGKLEPWRFIVVTGEGRErlAELLAAAAAARAPGADPEKLEKAR 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234    89 AYATlklqgidQAPLQLAVFTHRDPaaghglgRASMPVTLQQSTAMAFTRSGCRAGENLGLGMV----SVLDPKAVERLL 164
Cdd:cd02135  81 EKAL-------RAPVVIAVVAKPDE-------DPKVPEWEQYAAVGAAVQNLLLAAHALGLGAVwrtgPVTYDPAVREAL 146

                       170
                ....*....|....*
gi 609234   165 NAPPDWDFVAWLCIG 179
Cdd:cd02135 147 GLPEDERIVGFLYLG 161
nitroreductase cd02151
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 13-63 2.17e-09

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers..


Pssm-ID: 380326 [Multi-domain]  Cd Length: 157  Bit Score: 54.08  E-value: 2.17e-09
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|.
gi 609234    13 EVLRWRRDVRHFRPDPIDEAVIDRLRAVMDMAPSVGNARPWRVIRVDSPAL 63
Cdd:cd02151   1 ELLKKRRSIRKYTDEPIEEEKLEEILEAALLAPSSRNSRPVEFIVVDDKET 51
NfsA-like cd02146
nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin ...
 13-203 5.05e-08

nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin reductase and oxygen-insensitive nitroreductase. These enzymes are homodimeric flavoproteins that contain one FMN per monomer as a cofactor. Flavin reductase catalyzes the reduction of flavin by using NADPH as an electron donor. Oxygen-insensitive nitroreductase, such as NfsA protein in Escherichia coli, catalyzes reduction of nitrocompounds using NADPH as electron donor.


Pssm-ID: 380322 [Multi-domain]  Cd Length: 229  Bit Score: 51.47  E-value: 5.05e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234    13 EVLRWRRDVRHFRPDPIDEAVIDRLRAVMDMAPSVGNARPWRVIRVDSPALRAEVlanfnaaraaagsayageqAEayat 92
Cdd:cd02146   3 ETILNHRSVRKFTDEPLTDETLETLIAAAQSASTSSNLQAYSVIVVTDPELREKL-------------------AE---- 59

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234    93 lkLQG----IDQAPLQLaVF---THRDPAAGHGLGRASMPVTLQQSTAMAFTRSG------CRAGENLGLGMV---SVL- 155
Cdd:cd02146  60 --LAGnqpyVAQAPVFL-VFcadLYRHQKIAEEAGGKDVGLDYLESFLVGVVDAAlaaqnaLVAAESLGLGIVyigGIRn 136

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 609234   156 DPKAVERLLNAPPDWDFVAWLCIGVPeftDDTP----------LLHRAGWQENLPTEW 203
Cdd:cd02146 137 NPEEVIELLGLPEYVFPLFGLTVGHP---DPTPevkprlpleaVVHEETYQDLTDLED 191
nitroreductase cd02150
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 18-63 2.96e-07

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers.


Pssm-ID: 380325 [Multi-domain]  Cd Length: 156  Bit Score: 47.98  E-value: 2.96e-07
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*..
gi 609234    18 RRDVRHFRPDPIDEAVIDR-LRAVMdMAPSVGNARPWRVIRVDSPAL 63
Cdd:cd02150   4 RRSIRKYTDKPVEEEDIEKlLRAAM-AAPSAGNQQPWHFIVVTDREK 49
MhqN-like cd02137
nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily ...
 13-68 4.47e-07

nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily of the nitroreductase family containing uncharacterized proteins; includes nitroreductases MhqN, YodC, YdgI, DrgA. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380314 [Multi-domain]  Cd Length: 147  Bit Score: 47.62  E-value: 4.47e-07
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*..
gi 609234    13 EVLRWRRDVRHFRPD-PIDEAVIDRLRAVMDMAPSVGNARPWRVIRVDSPALRAEVL 68
Cdd:cd02137   2 EVIKSRRSVRNFDPDhKIPKEELKEILELATLAPSSFNLQPWRFVVVRDPELKAKLA 58
iodotyrosine_dehalogenase cd02144
iodotyrosine dehalogenase; Iodotyrosine dehalogenase catalyzes the removal of iodine from the ...
 13-67 5.10e-07

iodotyrosine dehalogenase; Iodotyrosine dehalogenase catalyzes the removal of iodine from the 3, 5 positions of L-tyosine in thyroid, liver and kidney, using NADPH as electron donor. This enzyme is a homolog of the nitroreductase family. These enzymes are usually homodimers.


Pssm-ID: 380320  Cd Length: 192  Bit Score: 47.92  E-value: 5.10e-07
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*
gi 609234    13 EVLRWRRDVRHFRPDPIDEAVIDRLRAVMDMAPSVGNARPWRVIRVDSPALRAEV 67
Cdd:cd02144   3 ELMKKRRSVRDFSSEPVPREVIENAIRTAGTAPSGANTQPWTFVVVSDPEIKRKI 57
nitroreductase cd20608
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 13-67 2.18e-06

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380329 [Multi-domain]  Cd Length: 145  Bit Score: 45.40  E-value: 2.18e-06
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*
gi 609234    13 EVLRWRRDVRHFRPDPIDEAVIDRLRAVMDMAPSVGNARPWRVIRVDSPALRAEV 67
Cdd:cd20608   2 EAIKTRRSVRRFSDKPVEEEKLEKILEAARLAPSWANKQCWRFIVVTDKETLSEL 56
nitroreductase cd20609
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 13-179 3.59e-06

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers.


Pssm-ID: 380330 [Multi-domain]  Cd Length: 145  Bit Score: 45.07  E-value: 3.59e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234    13 EVLRWRRDVRHFRPDPIDEAVIDR-LRAVMdMAPSVGNARPWRVIRVDSPALRAEVlanfnaaraaagsayageqAEAYA 91
Cdd:cd20609   4 ELAKKRYSVRKFSDKPVEKEKLDKiLEAGR-LAPTAVNYQPQRILVVRSEEALEKL-------------------AKATP 63

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 609234    92 TLKlqgidQAPLQLAVFTHRDPAAGHGLGRASMpvtLQQSTAMAFTRSgCRAGENLGLG--MVSVLDPKAVERLLNAPPD 169
Cdd:cd20609  64 RFF-----GAPLVIVVCYDKDESWKRPYDGKDS---GDIDAAIVATHM-MLAATELGLGtcWVGNFDPEKVREAFNLPEN 134

                       170
                ....*....|
gi 609234   170 WDFVAWLCIG 179
Cdd:cd20609 135 LEPVAILPLG 144
nitroreductase cd03370
uncharacterized nitroreductase family proteins; Nitroreductase family containing Thermus ...
 11-66 1.27e-05

uncharacterized nitroreductase family proteins; Nitroreductase family containing Thermus thermophilus NADH oxidase and other, uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380327 [Multi-domain]  Cd Length: 191  Bit Score: 44.23  E-value: 1.27e-05
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*.
gi 609234    11 LTEVLRWRRDVRHFRPDPIDEAVIDRLRAVMDMAPSVGNARPWRVIRVDSPALRAE 66
Cdd:cd03370   1 VKEAIESRRSIRKYTQEPVPDEDLREILRLAGLAPSAWNIQPWRFVVVRDAELKEQ 56
PnbA_NfnB-like cd02136
nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as ...
 18-63 1.70e-05

nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as a cofactor and catalyze reduction of a variety of nitroaromatic compounds, including nitrofurans, nitrobenzens, nitrophenol, nitrobenzoate and quinones by using either NADH or NADPH as a source of reducing equivalents in an obligatory two-election transfer mechanism. The enzyme is typically a homodimer. Mycobacterium smegmatis nitroreductase NfnB plays a role in resistance to benzothiazinone.


Pssm-ID: 380313 [Multi-domain]  Cd Length: 152  Bit Score: 43.35  E-value: 1.70e-05
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*..
gi 609234    18 RRDVRHFRPDPIDEAVIDR-LRAVMdMAPSVGNARPWRVIRVDSPAL 63
Cdd:cd02136   5 RRSVRAFKDKPVPKETIEKiLEAAR-RAPSGKNTQPWRVYVVTGKAR 50
nitroreductase_FeS-like cd02143
nitroreductases with an N-terminal iron-sulfur cluster-binding domain; Members of this family ...
 15-62 1.84e-05

nitroreductases with an N-terminal iron-sulfur cluster-binding domain; Members of this family utilize FMN as a cofactor. This family may be involved in the reduction of flavin or nitroaromatic compounds via an obligatory two-electron transfer. Nitroreductase is homodimer. Each subunit contains one FMN molecule.


Pssm-ID: 380319 [Multi-domain]  Cd Length: 187  Bit Score: 43.62  E-value: 1.84e-05
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*...
gi 609234    15 LRWRRDVRHFRPDPIDEAVIDRLRAVMDMAPSVGNARPWRVIRVDSPA 62
Cdd:cd02143   2 LRSRRSIRRYKDKPVPRETLEKLLDIARYAPTGHNSQPVHWLVVDDPE 49
PRK13294 PRK13294
F420-0--gamma-glutamyl ligase; Provisional
 4-68 3.96e-05

F420-0--gamma-glutamyl ligase; Provisional


Pssm-ID: 183957 [Multi-domain]  Cd Length: 448  Bit Score: 43.46  E-value: 3.96e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 609234      4 EQTHRDALTEvlrwRRDVRHFRPDPIDEAVIDRLRAVMDMAPSVGNARPWRVIRVDSPALRAEVL 68
Cdd:PRK13294 250 ELGRREAVLL----RRSVREFSDDPVDPEAVRRAVAAALTAPAPHHTRPVRFVWLRSAAVRTRLL 310
NfsB-like cd02149
nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This ...
 13-67 1.16e-03

nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This domain catalyzes the reduction of flavin, nitrocompound, quinones and azo compounds using NADH or NADPH as an electron donor. The enzyme is a homodimer, and each monomer binds a FMN as co-factor. This family includes FRase I in Vibrio fischeri, wihich reduces FMN into FMNH2 as part of the bioluminescent reaction. The family also includes oxygen-insensitive nitroreductases that use NADH or NADPH as an electron donor in the ping pong bi bi mechanism. This type of nitroreductase can be used in cancer chemotherapy to activate a range of prodrugs.


Pssm-ID: 380324 [Multi-domain]  Cd Length: 156  Bit Score: 38.00  E-value: 1.16e-03
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*.
gi 609234    13 EVLRWRRDVRHFRPD-PIDEAVIDRLRAVMDMAPSVGNARPWRVIRVDSPALRAEV 67
Cdd:cd02149   4 ELLNFRYATKKFDPNkKISDEDLETILEALRLSPSSFGLEPWKFLVVENPELKAKL 59
PRK05365 PRK05365
malonic semialdehyde reductase; Provisional
 9-65 4.46e-03

malonic semialdehyde reductase; Provisional


Pssm-ID: 180040 [Multi-domain]  Cd Length: 195  Bit Score: 36.72  E-value: 4.46e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 609234      9 DALTEVLRWRRDVRHFRPDPIDEAVIDRLRAVMDMAPSVGNARPWRVIRVDSPALRA 65
Cdd:PRK05365   7 AALDQLFTEARTHNGWLDEPVSDEQLRELYDLVKWGPTSANCSPARFVFVRSAEAKE 63
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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