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Conserved domains on  [gi|585177563|ref|XP_006740514|]
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isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial isoform X1 [Leptonychotes weddellii]

Protein Classification

NAD-dependent isocitrate dehydrogenase( domain architecture ID 10015520)

NAD-dependent isocitrate dehydrogenase converts isocitrate to 2-oxoglutarate in an NAD-dependent manner

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
mito_nad_idh TIGR00175
isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent ...
 51-382 0e+00

isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the multimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates. Mitochondrial NAD-dependent isocitrate dehydrogenases (IDH) resemble prokaryotic NADP-dependent IDH and 3-isopropylmalate dehydrogenase (an NAD-dependent enzyme) more closely than they resemble eukaryotic NADP-dependent IDH. The mitochondrial NAD-dependent isocitrate dehydrogenase is believed to be an alpha(2)-beta-gamma heterotetramer. All subunits are homologous and found by this model. The NADP-dependent IDH of Thermus aquaticus thermophilus strain HB8 resembles these NAD-dependent IDH, except for the residues involved in cofactor specificity, much more closely than it resembles other prokaryotic NADP-dependent IDH, including that of Thermus aquaticus strain YT1. [Energy metabolism, TCA cycle]


:

Pssm-ID: 272942  Cd Length: 333  Bit Score: 588.58  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563   51 GGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNAD--EEDIRNAIMAIRRNRVALKGNIETNHNLPpSHK 128
Cdd:TIGR00175   1 GGKYTVTLIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTDgkTEIPDEAVESIKRNKVALKGPLETPIGKG-GHR 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  129 SRNNILRTSLDLYANVIHCKSLPGVVTRHRDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQ 208
Cdd:TIGR00175  80 SLNVALRKELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYAR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  209 ESGRKKVTAVHKANIMKLGDGLFLQCCKEVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVG 288
Cdd:TIGR00175 160 KNGRKKVTAVHKANIMKLADGLFLNVCREVAKEYPDITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLVG 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  289 GPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQ 368
Cdd:TIGR00175 240 GPGLVPGANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLGLKEHADRIQKAVLSTIAEGKNRTKDLGGT 319

                         330
                  ....*....|....
gi 585177563  369 GTTSEAIQDIIRHI 382
Cdd:TIGR00175 320 ATTSDFTEAVIKRL 333
 
Name Accession Description Interval E-value
mito_nad_idh TIGR00175
isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent ...
 51-382 0e+00

isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the multimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates. Mitochondrial NAD-dependent isocitrate dehydrogenases (IDH) resemble prokaryotic NADP-dependent IDH and 3-isopropylmalate dehydrogenase (an NAD-dependent enzyme) more closely than they resemble eukaryotic NADP-dependent IDH. The mitochondrial NAD-dependent isocitrate dehydrogenase is believed to be an alpha(2)-beta-gamma heterotetramer. All subunits are homologous and found by this model. The NADP-dependent IDH of Thermus aquaticus thermophilus strain HB8 resembles these NAD-dependent IDH, except for the residues involved in cofactor specificity, much more closely than it resembles other prokaryotic NADP-dependent IDH, including that of Thermus aquaticus strain YT1. [Energy metabolism, TCA cycle]


Pssm-ID: 272942  Cd Length: 333  Bit Score: 588.58  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563   51 GGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNAD--EEDIRNAIMAIRRNRVALKGNIETNHNLPpSHK 128
Cdd:TIGR00175   1 GGKYTVTLIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTDgkTEIPDEAVESIKRNKVALKGPLETPIGKG-GHR 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  129 SRNNILRTSLDLYANVIHCKSLPGVVTRHRDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQ 208
Cdd:TIGR00175  80 SLNVALRKELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYAR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  209 ESGRKKVTAVHKANIMKLGDGLFLQCCKEVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVG 288
Cdd:TIGR00175 160 KNGRKKVTAVHKANIMKLADGLFLNVCREVAKEYPDITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLVG 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  289 GPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQ 368
Cdd:TIGR00175 240 GPGLVPGANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLGLKEHADRIQKAVLSTIAEGKNRTKDLGGT 319

                         330
                  ....*....|....
gi 585177563  369 GTTSEAIQDIIRHI 382
Cdd:TIGR00175 320 ATTSDFTEAVIKRL 333
PLN00123 PLN00123
isocitrate dehydrogenase (NAD+)
 30-382 1.34e-129

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215065  Cd Length: 360  Bit Score: 376.51  E-value: 1.34e-129
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  30 SAHEAPRRSVsqqTIPPSAKYGGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSnaDEEDIRNAIM-AIRR 108
Cdd:PLN00123  10 LGSKAQRRSV---TYMPRPGDGAPRAVTLIPGDGIGPLVTGAVEQVMEAMHAPVYFERYEVHG--DMKKVPEEVLeSIRR 84

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 109 NRVALKGNIETNHNLPPShkSRNNILRTSLDLYANVIHCKSLPGVVTRHRDIDILIVRENTEGEYSSLEHESVAGVVESL 188
Cdd:PLN00123  85 NKVCLKGGLATPVGGGVS--SLNVQLRKELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYSGLEHEVVPGVVESL 162

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 189 KIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCKEVAARYPQITFENMIVDNTTMQLVSRPQQFDV 268
Cdd:PLN00123 163 KVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAKKYPGIKYNEIIVDNCCMQLVSKPEQFDV 242

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 269 MVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATR--NTGK-SIANKNIANPTATLLASCMMLDHLKLHSYAT 345
Cdd:PLN00123 243 MVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVFEQGASagNVGNeKLVEQKKANPVALLLSSAMMLRHLQFPSFAD 322

                        330       340       350
                 ....*....|....*....|....*....|....*..
gi 585177563 346 SIRKAVLASMDNENMHTPDIGGQGTTSEAIQDIIRHI 382
Cdd:PLN00123 323 RLETAVKRVIAEGKYRTKDLGGSSTTQEVVDAVIANL 359
LeuB COG0473
Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid ...
 53-382 2.13e-117

Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid transport and metabolism]; Isocitrate/isopropylmalate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440241  Cd Length: 346  Bit Score: 345.07  E-value: 2.13e-117
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  53 RHTVTMIPGDGIGPELMLHVKSVFRHAC----VPVDFEEVHVSSNA--------DEEDIRnaimAIRRNRVALKGNIETN 120
Cdd:COG0473    1 TYKIAVLPGDGIGPEVVAAALKVLEAAAerfgLDFEFEEADIGGAAydktgtplPDETLE----ALRKADAILLGAVGGP 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 121 HnlPPSHKSRNNI---LRTSLDLYANVIHCKSLPGVVTRHR-----DIDILIVRENTEGEYSSLEHESVAG----VVESL 188
Cdd:COG0473   77 K--WDDGVRPESGllaLRKELDLYANLRPAKLYPGLPSPLKpeiveGIDLVIVRENTEGLYFGIGGRIGTGtgeeVAIDT 154

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 189 KIITKAKSLRIAEYAFKLAQESgRKKVTAVHKANIMKLGDGLFLQCCKEVAARYPQITFENMIVDNTTMQLVSRPQQFDV 268
Cdd:COG0473  155 RVYTRKGIERIARYAFELARKR-RKKVTSVDKANVLKLTSGLWREVVREVAKEYPDVELDHMYVDAAAMQLVRNPEQFDV 233

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 269 MVMPNLYGNIVNNVCAGLVGGPGLVAGANYG-HVYAVFE----TATRntgksIANKNIANPTATLLASCMMLDHLKLHSY 343
Cdd:COG0473  234 IVTENLFGDILSDLAAGLTGSLGLAPSANIGdEGKALFEpvhgSAPD-----IAGKGIANPIATILSAAMMLRHLGEEEA 308

                        330       340       350
                 ....*....|....*....|....*....|....*....
gi 585177563 344 ATSIRKAVLASMDnENMHTPDIGGQGTTSEAIQDIIRHI 382
Cdd:COG0473  309 ADAIEAAVEKVLA-EGVRTPDLGGKAGTSEMGDAIIAAL 346
Iso_dh pfam00180
Isocitrate/isopropylmalate dehydrogenase;
55-378 9.43e-92

Isocitrate/isopropylmalate dehydrogenase;


Pssm-ID: 425507  Cd Length: 346  Bit Score: 279.56  E-value: 9.43e-92
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563   55 TVTMIPGDGIGPELMLHVKSVFRHACVPV----DFEEVHVSSNA-DEEDI---RNAIMAIRRNRVALKGNIETNHNLPPS 126
Cdd:pfam00180   1 KIAVLPGDGIGPEVMAAALKVLKAALEKAplefEFEERDVGGAAiDETGEplpDETLEACKKADAVLLGAVGGPKWDPAG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  127 HKSRNNI--LRTSLDLYANVIHCKSLPGVVT------RHRDIDILIVRENTEGEYSSLEHE---SVAGVVESLKIITKAK 195
Cdd:pfam00180  81 VRPENGLlaLRKELGLFANLRPAKVFPPLGDasplknEVEGVDIVIVRELTGGIYFGIEKGikgSGNEVAVDTKLYSRDE 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  196 SLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCKEVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLY 275
Cdd:pfam00180 161 IERIARVAFELARKRGRKKVTSVDKANVLKSSDLWRKIVTEVAKAEYPDVELEHQLVDNAAMQLVKNPSQFDVIVTPNLF 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  276 GNIVNNVCAGLVGGPGLVAGANYG-HVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDH-LKLHSYATSIRKAVLA 353
Cdd:pfam00180 241 GDILSDEASMLTGSLGLLPSASLGaNGFGIFE-PVHGSAPDIAGKGIANPIATILSAAMMLRYsLGLEDAADKIEAAVLK 319

                         330       340
                  ....*....|....*....|....*...
gi 585177563  354 SMDNENMhTPDIGGQGT---TSEAIQDI 378
Cdd:pfam00180 320 VLESGIR-TGDLAGSATyvsTSEFGEAV 346
 
Name Accession Description Interval E-value
mito_nad_idh TIGR00175
isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent ...
 51-382 0e+00

isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the multimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates. Mitochondrial NAD-dependent isocitrate dehydrogenases (IDH) resemble prokaryotic NADP-dependent IDH and 3-isopropylmalate dehydrogenase (an NAD-dependent enzyme) more closely than they resemble eukaryotic NADP-dependent IDH. The mitochondrial NAD-dependent isocitrate dehydrogenase is believed to be an alpha(2)-beta-gamma heterotetramer. All subunits are homologous and found by this model. The NADP-dependent IDH of Thermus aquaticus thermophilus strain HB8 resembles these NAD-dependent IDH, except for the residues involved in cofactor specificity, much more closely than it resembles other prokaryotic NADP-dependent IDH, including that of Thermus aquaticus strain YT1. [Energy metabolism, TCA cycle]


Pssm-ID: 272942  Cd Length: 333  Bit Score: 588.58  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563   51 GGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNAD--EEDIRNAIMAIRRNRVALKGNIETNHNLPpSHK 128
Cdd:TIGR00175   1 GGKYTVTLIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTDgkTEIPDEAVESIKRNKVALKGPLETPIGKG-GHR 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  129 SRNNILRTSLDLYANVIHCKSLPGVVTRHRDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQ 208
Cdd:TIGR00175  80 SLNVALRKELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYAR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  209 ESGRKKVTAVHKANIMKLGDGLFLQCCKEVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVG 288
Cdd:TIGR00175 160 KNGRKKVTAVHKANIMKLADGLFLNVCREVAKEYPDITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLVG 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  289 GPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQ 368
Cdd:TIGR00175 240 GPGLVPGANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLGLKEHADRIQKAVLSTIAEGKNRTKDLGGT 319

                         330
                  ....*....|....
gi 585177563  369 GTTSEAIQDIIRHI 382
Cdd:TIGR00175 320 ATTSDFTEAVIKRL 333
PLN00123 PLN00123
isocitrate dehydrogenase (NAD+)
 30-382 1.34e-129

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215065  Cd Length: 360  Bit Score: 376.51  E-value: 1.34e-129
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  30 SAHEAPRRSVsqqTIPPSAKYGGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSnaDEEDIRNAIM-AIRR 108
Cdd:PLN00123  10 LGSKAQRRSV---TYMPRPGDGAPRAVTLIPGDGIGPLVTGAVEQVMEAMHAPVYFERYEVHG--DMKKVPEEVLeSIRR 84

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 109 NRVALKGNIETNHNLPPShkSRNNILRTSLDLYANVIHCKSLPGVVTRHRDIDILIVRENTEGEYSSLEHESVAGVVESL 188
Cdd:PLN00123  85 NKVCLKGGLATPVGGGVS--SLNVQLRKELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYSGLEHEVVPGVVESL 162

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 189 KIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCKEVAARYPQITFENMIVDNTTMQLVSRPQQFDV 268
Cdd:PLN00123 163 KVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAKKYPGIKYNEIIVDNCCMQLVSKPEQFDV 242

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 269 MVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATR--NTGK-SIANKNIANPTATLLASCMMLDHLKLHSYAT 345
Cdd:PLN00123 243 MVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVFEQGASagNVGNeKLVEQKKANPVALLLSSAMMLRHLQFPSFAD 322

                        330       340       350
                 ....*....|....*....|....*....|....*..
gi 585177563 346 SIRKAVLASMDNENMHTPDIGGQGTTSEAIQDIIRHI 382
Cdd:PLN00123 323 RLETAVKRVIAEGKYRTKDLGGSSTTQEVVDAVIANL 359
PLN00118 PLN00118
isocitrate dehydrogenase (NAD+)
 21-382 2.11e-125

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215062  Cd Length: 372  Bit Score: 366.51  E-value: 2.11e-125
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  21 LLCRPWEVLSAHEAPRRSVSQQTIPPSAK----YGGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADE 96
Cdd:PLN00118   5 LLRRLLGNRLAQILGASSSSSGAFSSSARafssSSTPITATLFPGDGIGPEIAESVKQVFTAAGVPIEWEEHYVGTTVDP 84

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  97 ED----IRNAIMAIRRNRVALKGNIETNhnLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHRDIDILIVRENTEGE 172
Cdd:PLN00118  85 RTgsflTWESLESVRRNKVGLKGPMATP--IGKGHRSLNLTLRKELGLYANVRPCYSLPGYKTRYDDVDLVTIRENTEGE 162

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 173 YSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCKEVAARYPQITFENMIV 252
Cdd:PLN00118 163 YSGLEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTHGRKRVSAIHKANIMKKTDGLFLKCCREVAEKYPEIVYEEVII 242

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 253 DNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASC 332
Cdd:PLN00118 243 DNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGENGLALAEAVHGSAPDIAGKNLANPTALLLSAV 322

                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|
gi 585177563 333 MMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSEAIQDIIRHI 382
Cdd:PLN00118 323 MMLRHLKLNEQAEQIHNAILNTIAEGKYRTADLGGSSTTTDFTKAICDHL 372
LeuB COG0473
Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid ...
 53-382 2.13e-117

Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid transport and metabolism]; Isocitrate/isopropylmalate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440241  Cd Length: 346  Bit Score: 345.07  E-value: 2.13e-117
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  53 RHTVTMIPGDGIGPELMLHVKSVFRHAC----VPVDFEEVHVSSNA--------DEEDIRnaimAIRRNRVALKGNIETN 120
Cdd:COG0473    1 TYKIAVLPGDGIGPEVVAAALKVLEAAAerfgLDFEFEEADIGGAAydktgtplPDETLE----ALRKADAILLGAVGGP 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 121 HnlPPSHKSRNNI---LRTSLDLYANVIHCKSLPGVVTRHR-----DIDILIVRENTEGEYSSLEHESVAG----VVESL 188
Cdd:COG0473   77 K--WDDGVRPESGllaLRKELDLYANLRPAKLYPGLPSPLKpeiveGIDLVIVRENTEGLYFGIGGRIGTGtgeeVAIDT 154

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 189 KIITKAKSLRIAEYAFKLAQESgRKKVTAVHKANIMKLGDGLFLQCCKEVAARYPQITFENMIVDNTTMQLVSRPQQFDV 268
Cdd:COG0473  155 RVYTRKGIERIARYAFELARKR-RKKVTSVDKANVLKLTSGLWREVVREVAKEYPDVELDHMYVDAAAMQLVRNPEQFDV 233

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 269 MVMPNLYGNIVNNVCAGLVGGPGLVAGANYG-HVYAVFE----TATRntgksIANKNIANPTATLLASCMMLDHLKLHSY 343
Cdd:COG0473  234 IVTENLFGDILSDLAAGLTGSLGLAPSANIGdEGKALFEpvhgSAPD-----IAGKGIANPIATILSAAMMLRHLGEEEA 308

                        330       340       350
                 ....*....|....*....|....*....|....*....
gi 585177563 344 ATSIRKAVLASMDnENMHTPDIGGQGTTSEAIQDIIRHI 382
Cdd:COG0473  309 ADAIEAAVEKVLA-EGVRTPDLGGKAGTSEMGDAIIAAL 346
PRK08997 PRK08997
isocitrate dehydrogenase; Provisional
 55-382 2.43e-108

isocitrate dehydrogenase; Provisional


Pssm-ID: 181606  Cd Length: 334  Bit Score: 321.67  E-value: 2.43e-108
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  55 TVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDI----RNAIMAIRRNRVALKGNIETNhnLPPSHKSR 130
Cdd:PRK08997   4 TITVIPGDGIGPSIIDATLKILDKLGCDFEYEFADAGLTALEKHGellpQRTLDLIEKNKIALKGPLTTP--VGEGFTSI 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 131 NNILRTSLDLYANVIHCKSLPGVVTRHRDIDILIVRENTEGEYSSLEHE-SVAG-VVESLKIITKAKSLRIAEYAFKLAQ 208
Cdd:PRK08997  82 NVTLRKKFDLYANVRPVLSFPGTKARYDNIDIITVRENTEGMYSGEGQTvSEDGeTAEATSIITRKGAERIVRFAYELAR 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 209 ESGRKKVTAVHKANIMKLGDGLFLQCCKEVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVG 288
Cdd:PRK08997 162 KEGRKKVTAVHKANIMKSTSGLFLKVAREVALRYPDIEFEEMIVDATCMQLVMNPEQFDVIVTTNLFGDILSDLCAGLVG 241

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 289 GPGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQ 368
Cdd:PRK08997 242 GLGMAPGANIGRDAAIFE-AVHGSAPDIAGKNLANPTSVILAAIQMLEYLGMPDKAERIRKAIVAVIEAGDRTTRDLGGT 320

                        330
                 ....*....|....
gi 585177563 369 GTTSEAIQDIIRHI 382
Cdd:PRK08997 321 HGTTDFTQAVIDRL 334
PRK09222 PRK09222
NADP-dependent isocitrate dehydrogenase;
56-373 4.65e-101

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 236416 [Multi-domain]  Cd Length: 482  Bit Score: 307.97  E-value: 4.65e-101
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  56 VTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDIRNAIM-----AIRRNRVALKGNIETnhnlpP---SH 127
Cdd:PRK09222   7 ITVAYGDGIGPEIMEAVLKILEAAGAPLEIETIEIGEKVYKKGWTSGISpsaweSIRRTKVLLKAPITT-----PqggGY 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 128 KSRNNILRTSLDLYANVIHCKSL-PGVVTRHRDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKL 206
Cdd:PRK09222  82 KSLNVTLRKTLGLYANVRPCVSYhPFVETKHPNLDVVIIRENEEDLYAGIEHRQTPDVYQCLKLISRPGSEKIIRYAFEY 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 207 AQESGRKKVTAVHKANIMKLGDGLFLQCCKEVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGL 286
Cdd:PRK09222 162 ARANGRKKVTCLTKDNIMKLTDGLFHKVFDEIAKEYPDIEAEHYIVDIGAARLATNPENFDVIVTPNLYGDILSDIAAEI 241

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 287 VGGPGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDnENMHTPDIG 366
Cdd:PRK09222 242 SGSVGLAGSANIGEEYAMFE-AVHGSAPDIAGKNIANPSGLLNAAVMMLVHIGQFDIAELIENAWLKTLE-DGIHTADIY 319


                 ....*..
gi 585177563 367 GQGTTSE 373
Cdd:PRK09222 320 NEGVSKK 326
Iso_dh pfam00180
Isocitrate/isopropylmalate dehydrogenase;
55-378 9.43e-92

Isocitrate/isopropylmalate dehydrogenase;


Pssm-ID: 425507  Cd Length: 346  Bit Score: 279.56  E-value: 9.43e-92
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563   55 TVTMIPGDGIGPELMLHVKSVFRHACVPV----DFEEVHVSSNA-DEEDI---RNAIMAIRRNRVALKGNIETNHNLPPS 126
Cdd:pfam00180   1 KIAVLPGDGIGPEVMAAALKVLKAALEKAplefEFEERDVGGAAiDETGEplpDETLEACKKADAVLLGAVGGPKWDPAG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  127 HKSRNNI--LRTSLDLYANVIHCKSLPGVVT------RHRDIDILIVRENTEGEYSSLEHE---SVAGVVESLKIITKAK 195
Cdd:pfam00180  81 VRPENGLlaLRKELGLFANLRPAKVFPPLGDasplknEVEGVDIVIVRELTGGIYFGIEKGikgSGNEVAVDTKLYSRDE 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  196 SLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCKEVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLY 275
Cdd:pfam00180 161 IERIARVAFELARKRGRKKVTSVDKANVLKSSDLWRKIVTEVAKAEYPDVELEHQLVDNAAMQLVKNPSQFDVIVTPNLF 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  276 GNIVNNVCAGLVGGPGLVAGANYG-HVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDH-LKLHSYATSIRKAVLA 353
Cdd:pfam00180 241 GDILSDEASMLTGSLGLLPSASLGaNGFGIFE-PVHGSAPDIAGKGIANPIATILSAAMMLRYsLGLEDAADKIEAAVLK 319

                         330       340
                  ....*....|....*....|....*...
gi 585177563  354 SMDNENMhTPDIGGQGT---TSEAIQDI 378
Cdd:pfam00180 320 VLESGIR-TGDLAGSATyvsTSEFGEAV 346
PRK14025 PRK14025
multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase; Provisional
 54-382 5.03e-82

multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase; Provisional


Pssm-ID: 184462  Cd Length: 330  Bit Score: 253.90  E-value: 5.03e-82
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  54 HTVTMIPGDGIGPELMLHVKSVFRhaCVPVDFEEVHVSSnADEEDIR-------NAIMAIRRNRVALKGNIETnhnlppS 126
Cdd:PRK14025   2 HKICVIEGDGIGKEVVPAALHVLE--ATGLPFEFVYAEA-GDEVFEKtgkalpeETIEAAKEADAVLFGAAGE------T 72

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 127 HKSRNNILRTSLDLYANVIHCKSLPGVVTRHRDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKL 206
Cdd:PRK14025  73 AADVIVKLRRILDTYANVRPVKSYKGVKCLYPDIDYVIVRENTEGLYKGIEAEIADGVTVATRVITRKASERIFRFAFEM 152

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 207 AQ----ESGRKKVTAVHKANIMKLGDGLFLQCCKEVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNV 282
Cdd:PRK14025 153 AKrrkkMGKEGKVTCAHKANVLKKTDGLFKKTFYEVAKEYPDIKAEDYYVDAMNMYIITRPQTFDVVVTSNLFGDILSDG 232

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 283 CAGLVGGPGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDnENMHT 362
Cdd:PRK14025 233 AAGLVGGLGLAPSANIGDKYGLFE-PVHGSAPDIAGKGIANPTATILTAVLMLRHLGENEEADKVEKALEEVLA-LGLTT 310

                        330       340
                 ....*....|....*....|
gi 585177563 363 PDIGGQGTTSEAIQDIIRHI 382
Cdd:PRK14025 311 PDLGGNLSTMEMAEEVAKRV 330
LEU3_arch TIGR02088
isopropylmalate/isohomocitrate dehydrogenases; This model represents a group of archaeal ...
 56-379 4.49e-79

isopropylmalate/isohomocitrate dehydrogenases; This model represents a group of archaeal decarboxylating dehydrogenases which include the leucine biosynthesis enzyme 3-isopropylmalate dehydrogenase (LeuB, LEU3) and the methanogenic cofactor CoB biosynthesis enzyme isohomocitrate dehydrogenase (AksF). Both of these have been characterized in Methanococcus janaschii. Non-methanogenic archaea have only one hit to this model and presumably this is LeuB, although phylogenetic trees cannot establish which gene is which in the methanogens. The AksF gene is capable of acting on isohomocitrate, iso(homo)2-citrate and iso(homo)3-citrate in the successive elongation cycles of coenzyme B (7-mercaptoheptanoyl-threonine phosphate). This family is closely related to both the LeuB genes found in TIGR00169 and the mitochondrial eukaryotic isocitrate dehydratases found in TIGR00175. All of these are included within the broader subfamily model, pfam00180.


Pssm-ID: 273962  Cd Length: 322  Bit Score: 246.21  E-value: 4.49e-79
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563   56 VTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVH--------VSSNADEEDIRnaimAIRRNRVALKGNIETNhnLPPSH 127
Cdd:TIGR02088   1 VAVIPGDGIGPEVIEAAIRILNKLGLEIEFIEFEagdealkkYGSALPEDTLE----EIRKADAILFGAVTTP--ANPGY 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  128 KSRNNILRTSLDLYANVIHCKSLPGVVTRH-RDIDILIVRENTEGEYSSLEhESVAGVVESLKIITKAKSLRIAEYAFKL 206
Cdd:TIGR02088  75 KSVIVTLRKELDLYANVRPAKSLPGIPDLYpNGKDIVIVRENTEGLYAGFE-FGFSDRAIAIRVITREGSERIARFAFNL 153

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  207 AQESGRKkVTAVHKANIMKLGDGLFLQCCKEVAARYpQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGL 286
Cdd:TIGR02088 154 AKERNRK-VTCVHKANVLKGTDGLFREVCREIAKRY-GVEYRDMYVDSAAMNLVKDPWRFDVIVTTNMFGDILSDLASAL 231

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  287 VGGPGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMhTPDIG 366
Cdd:TIGR02088 232 AGSLGLAPSANIGDRKALFE-PVHGSAPDIAGKGIANPTAAILSVAMMLDYLGELEKGKLVWEAVEYYIIEGKK-TPDLG 309

                         330
                  ....*....|...
gi 585177563  367 GQGTTSEAIQDII 379
Cdd:TIGR02088 310 GTAKTKEVGDEIA 322
ICDH_alpha TIGR02924
isocitrate dehydrogenase; This family of mainly alphaproteobacterial enzymes is a member of ...
 56-365 9.50e-79

isocitrate dehydrogenase; This family of mainly alphaproteobacterial enzymes is a member of the isocitrate/isopropylmalate dehydrogenase superfamily described by pfam00180. Every member of the seed of this model appears to have a TCA cycle lacking only a determined isocitrate dehydrogenase. The precise identity of the cofactor (NADH -- 1.1.1.41 vs. NADPH -- 1.1.1.42) is unclear. [Energy metabolism, TCA cycle]


Pssm-ID: 274353 [Multi-domain]  Cd Length: 473  Bit Score: 250.06  E-value: 9.50e-79
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563   56 VTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDIRNAIM-----AIRRNRVALKGNIETNHNlpPSHKSR 130
Cdd:TIGR02924   3 ITVAYGDGIGPEIMEAVLLILKEAEAPIDIETIEIGEKVYKKGWPSGISpssweSIRRTKVLLKAPITTPQG--GGHKSL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  131 NNILRTSLDLYANVIHCKSL-PGVVTRHRDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQE 209
Cdd:TIGR02924  81 NVTLRKTLGLYANIRPCVSYhPFIETKSPNLNIVIVRENEEDLYTGIEYRQTPDTYECTKLITRSGSEKICRYAFEYARK 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  210 SGRKKVTAVHKANIMKLGDGLFLQCCKEVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGG 289
Cdd:TIGR02924 161 HNRKKVTCLTKDNIMKMTDGIFHKIFDKIAAEYPDIESEHYIVDIGMARLATNPENFDVIVTPNLYGDILSDVAAEISGS 240

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 585177563  290 PGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDnENMHTPDI 365
Cdd:TIGR02924 241 VGLAGSANIGEEYAMFE-AVHGSAPDIAGQNIANPSGLLNAAIQMLVHIGQSDIAQLIYNAWLKTLE-DGVHTADI 314
PRK00772 PRK00772
3-isopropylmalate dehydrogenase; Provisional
 54-383 9.60e-55

3-isopropylmalate dehydrogenase; Provisional


Pssm-ID: 234832  Cd Length: 358  Bit Score: 184.53  E-value: 9.60e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  54 HTVTMIPGDGIGPELM---LHV-KSVFRHACVPVDFEEVHVSSNA-DE--EDIRNAIMAIRRNRVA-LKGNIETNH--NL 123
Cdd:PRK00772   3 YKIAVLPGDGIGPEVMaeaVKVlDAVAEKFGFDFEFEEALVGGAAiDAhgVPLPEETLEACRAADAvLLGAVGGPKwdNL 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 124 PPSHKSRNNIL--RTSLDLYAN---------VIHCKSL-PGVVtrhRDIDILIVRENTEGEY-------SSLEHESVAgv 184
Cdd:PRK00772  83 PPDVRPERGLLalRKELGLFANlrpaklypgLADASPLkPEIV---AGLDILIVRELTGGIYfgeprgrEGLGGEERA-- 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 185 VESLkIITKAKSLRIAEYAFKLAQESgRKKVTAVHKANIMKlGDGLFLQCCKEVAARYPQITFENMIVDNTTMQLVSRPQ 264
Cdd:PRK00772 158 FDTM-VYTREEIERIARVAFELARKR-RKKVTSVDKANVLE-SSRLWREVVTEVAKEYPDVELSHMYVDNAAMQLVRNPK 234

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 265 QFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHV-YAVFETAtrntGKS---IANKNIANPTATLLASCMMLDH-LK 339
Cdd:PRK00772 235 QFDVIVTENLFGDILSDEAAMLTGSLGMLPSASLGESgPGLYEPI----HGSapdIAGKGIANPIATILSAAMMLRYsLG 310

                        330       340       350       360
                 ....*....|....*....|....*....|....*....|....*..
gi 585177563 340 LHSYATSIRKAVLASMDnENMHTPDI---GGQGTTSEAIQDIIRHIR 383
Cdd:PRK00772 311 LEEAADAIEAAVEKVLA-QGYRTADIaegGGKVSTSEMGDAILAALA 356
leuB TIGR00169
3-isopropylmalate dehydrogenase; Several NAD- or NADP-dependent dehydrogenases, including ...
 56-382 1.32e-46

3-isopropylmalate dehydrogenase; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the dimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates.Among these decarboxylating dehydrogenases of hydroxyacids, overall sequence homology indicates evolutionary history rather than actual substrate or cofactor specifity, which may be toggled experimentally by replacement of just a few amino acids. 3-isopropylmalate dehydrogenase is an NAD-dependent enzyme and should have a sequence resembling HGSAPDI around residue 340. The subtrate binding loop should include a sequence resembling E[KQR]X(0,1)LLXXR around residue 115. Other contacts of importance are known from crystallography but not detailed here.This model will not find all isopropylmalate dehydrogenases; the enzyme from Sulfolobus sp. strain 7 is more similar to mitochondrial NAD-dependent isocitrate dehydrogenases than to other known isopropylmalate dehydrogenases and was omitted to improve the specificity of the model. It scores below the cutoff and below some enzymes known not to be isopropylmalate dehydrogenase. [Amino acid biosynthesis, Pyruvate family]


Pssm-ID: 272939  Cd Length: 346  Bit Score: 162.57  E-value: 1.32e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563   56 VTMIPGDGIGPELM---LHV-KSVFRHACVPVDFEEVHVSSNADE---EDIRNAIMAIRRNRVA-LKGNIETNH--NLPP 125
Cdd:TIGR00169   2 IAVLPGDGIGPEVMaqaLKVlKAVAERFGLKFEFEEHLIGGAAIDatgQPLPEETLKGCKEADAvLLGAVGGPKwdNLPR 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  126 SHKSRNNIL--RTSLDLYANVIHCKSLPGVVTRH-------RDIDILIVRENTEGEYSSLEHE-SVAGVVESLKIITKAK 195
Cdd:TIGR00169  82 DQRPEQGLLklRKSLDLFANLRPAKVFPGLEDLSplkeeiaKGVDFVVVRELTGGIYFGEPKGrEGEGEAWDTEVYTVPE 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  196 SLRIAEYAFKLAQESgRKKVTAVHKANIMKLGDgLFLQCCKEVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLY 275
Cdd:TIGR00169 162 IERIARVAFEMARKR-RKKVTSVDKANVLESSR-LWRKTVEEIAKEYPDVELEHQYIDNAAMQLVKSPTQFDVVVTSNLF 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  276 GNIVNNVCAGLVGGPGLVAGANYGH-VYAVFETAtRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLAS 354
Cdd:TIGR00169 240 GDILSDEASVITGSLGMLPSASLGSdGFGLFEPV-HGSAPDIAGKGIANPIAQILSAAMLLRYSFNLEEAADAIEAAVKK 318

                         330       340
                  ....*....|....*....|....*...
gi 585177563  355 MDNENMHTPDIGGQGTTSEAIQDIIRHI 382
Cdd:TIGR00169 319 VLAEGYRTPDLGSSATTAVGTAEMGEEL 346
PRK08194 PRK08194
tartrate dehydrogenase; Provisional
 55-383 1.29e-45

tartrate dehydrogenase; Provisional


Pssm-ID: 181281  Cd Length: 352  Bit Score: 160.27  E-value: 1.29e-45
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  55 TVTMIPGDGIGPELMLHVKSVFR-----HACVPVDFEEVHVSSnadEEDIRNAIMAIRRNRVALKgNIET-------NHN 122
Cdd:PRK08194   5 KIAVIPGDGVGKEVVPAAVRVLKavaevHGGLKFEFTEFPWSC---EYYLEHGEMMPEDGLEQLK-QFDAiflgavgNPK 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 123 LPPSHKSRNNIL---RTSLDLYANVIHCKSLPGV---VTRHRDIDILIVRENTEGEYSSL-------EHEsvagVVESLK 189
Cdd:PRK08194  81 LVPDHISLWGLLikiRREFEQVINIRPAKQLRGIkspLANPKDFDLLVVRENSEGEYSEVggrihrgEDE----IAIQNA 156

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 190 IITKAKSLRIAEYAFKLAQESgRKKVTAVHKAN----IMKLGDGLFlqccKEVAARYPQITFENMIVDNTTMQLVSRPQQ 265
Cdd:PRK08194 157 VFTRKGTERAMRYAFELAAKR-RKHVTSATKSNgivhSMPFWDEVF----QEVGKDYPEIETDSQHIDALAAFFVTRPEE 231

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 266 FDVMVMPNLYGNIVNNVCAGLVGGPGLVAGAN---YGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKlHS 342
Cdd:PRK08194 232 FDVIVASNLFGDILTDIGAAIMGSIGIAPAANinvNGKYPSMFE-PVHGSAPDIAGKGIANPIGQIWTAKLMLDHFG-EE 309

                        330       340       350       360
                 ....*....|....*....|....*....|....*....|.
gi 585177563 343 YATSIRKAVLASMDNENMHTPDIGGQGTTSEAIQDIIRHIR 383
Cdd:PRK08194 310 ELGSHLLDVIEDVTEDGIKTPDIGGRATTDEVTDEIISRLK 350
PRK03437 PRK03437
3-isopropylmalate dehydrogenase; Provisional
 58-366 3.96e-45

3-isopropylmalate dehydrogenase; Provisional


Pssm-ID: 179579  Cd Length: 344  Bit Score: 158.94  E-value: 3.96e-45
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  58 MIPGDGIGPELMLHVKSVFRHACVP-VDFEEVH-------------VSSNADEEDIR--NAIM--AIRRNRVAlKGNIET 119
Cdd:PRK03437   9 VIPGDGIGPEVVAEALKVLDAVAAGgPGVETTEydlgarrylrtgeTLPDSVLAELRqhDAILlgAIGDPSVP-SGVLER 87

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 120 NHNLPpshksrnniLRTSLDLYANVIHCKSLPGVVT---RHRDIDILIVRENTEGEYSS--------LEHEsVAGVVEsl 188
Cdd:PRK03437  88 GLLLK---------LRFALDHYVNLRPSKLYPGVTSplaGPGDIDFVVVREGTEGPYTGnggalrvgTPHE-VATEVS-- 155

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 189 kIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCKEVAARYPQITFENMIVDNTTMQLVSRPQQFDV 268
Cdd:PRK03437 156 -VNTAFGVERVVRDAFERAQKRPRKHLTLVHKTNVLTFAGDLWQRTVDEVAAEYPDVTVDYQHVDAATIFMVTDPSRFDV 234

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 269 MVMPNLYGNIVNNVCAGLVGGPGLVAGANY---GHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYAT 345
Cdd:PRK03437 235 IVTDNLFGDIITDLAAAVTGGIGLAASGNInptGTNPSMFE-PVHGSAPDIAGQGIADPTAAILSVALLLDHLGEEDAAA 313

                        330       340
                 ....*....|....*....|....
gi 585177563 346 SIRKAV---LASMDNENMHTPDIG 366
Cdd:PRK03437 314 RIEAAVeadLAERGKMGRSTAEVG 337
Icd COG0538
Isocitrate dehydrogenase [Energy production and conversion]; Isocitrate dehydrogenase is part ...
 59-383 3.91e-43

Isocitrate dehydrogenase [Energy production and conversion]; Isocitrate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440304  Cd Length: 409  Bit Score: 154.88  E-value: 3.91e-43
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  59 IPGDGIGPELMLHVKSVFRHA-------CVPVDFEEVHVS-SNADEEDIR---NAIMAIRRNRVALKGNIETnhnlpP-- 125
Cdd:COG0538   24 IEGDGIGPEITRAIWKVIDAAvekayggKRDIEWKEVDAGeKARDETGDWlpdETAEAIKEYGVGIKGPLTT-----Pvg 98

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 126 -SHKSRNNILRTSLDLYAN---VIHCKSLPGVVTRHRDIDILIVRENTEGEYSSLEHES----VAGVVESL--------- 188
Cdd:COG0538   99 gGWRSLNVTIRQILDLYVCrrpVRYFKGVPSPVKHPEKVDIVIFRENTEDIYAGIEWKAgspeALKLIFFLedemgvtvi 178

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 189 ----------KIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCKEVAAR-YP-------------- 243
Cdd:COG0538  179 rfpedsgigiKPVSDEGTERLVRAAIQYALDNKRKSVTLVHKGNIMKFTEGAFKDWGYEVAEEeFGdkfitegpwekykg 258

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 244 -----QITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYG-HVYAVFE----TATRntg 313
Cdd:COG0538  259 pkpagKIVYKDRIADDMLQQILLRPGEYDVIATKNLNGDYISDALAAQVGGLGIAPGANIGdDGGAEFEathgTAPK--- 335

                        330       340       350       360       370       380       390
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 585177563 314 ksIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMhTPD----IGGQG--TTSEAIQDIIRHIR 383
Cdd:COG0538  336 --YAGKDSTNPGSLILSGTMMLRHRGWLEAADLIEKAVEKTIESGKV-TYDlarlMEGATelSTSEFGDAIIENLD 408
PRK06451 PRK06451
NADP-dependent isocitrate dehydrogenase;
59-365 4.01e-42

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 235803  Cd Length: 412  Bit Score: 152.68  E-value: 4.01e-42
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  59 IPGDGIGPELMLHVKSVFRHACV-------PVDFEEVHVSSNADEED----IRNAIMAIRRNRVALKGNIETNhnLPPSH 127
Cdd:PRK06451  29 VEGDGIGPEITHAAMKVINKAVEkaygsdrEIKWVEVLAGDKAEKLTgnrfPKESEELIEKYRVLLKGPLETP--IGKGW 106

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 128 KSRNNILRTSLDLYANVIHCKSLPGVVT--RHRD-IDILIVRENTEGEYSSLEH----------------------ESVA 182
Cdd:PRK06451 107 KSINVAIRLMLDLYANIRPVKYIPGIESplKNPEkIDLIIFRENTDDLYRGIEYpydseeakkirdflrkelgvevEDDT 186

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 183 GVveSLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCKEVAAR--------------------- 241
Cdd:PRK06451 187 GI--GIKLISKFKTQRIARMAIKYAIDHKRKKVTIMHKGNVMKYTEGAFREWAYEVALKefrdyvvteeevtknyngvpp 264

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 242 YPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFEtATRNTGKSIANKNI 321
Cdd:PRK06451 265 SGKVIINDRIADNMFQQIIIRPDEYDIILAPNVNGDYISDAAGALVGNIGMLGGANIGDTGGMFE-AIHGTAPKYAGKNV 343

                        330       340       350       360
                 ....*....|....*....|....*....|....*....|....
gi 585177563 322 ANPTATLLASCMMLDHLKLHSYATSIRKAVLASMdNENMHTPDI 365
Cdd:PRK06451 344 ANPTGIIKGGELMLRFMGWDKAADLIDKAIMESI-KQKKVTQDL 386
PRK07006 PRK07006
isocitrate dehydrogenase; Reviewed
 105-351 2.76e-31

isocitrate dehydrogenase; Reviewed


Pssm-ID: 180792  Cd Length: 409  Bit Score: 122.86  E-value: 2.76e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 105 AIRRNRVALKGNIETnhnlpP---SHKSRNNILRTSLDLYA---NVIHCKSLPGVVTRHRDIDILIVRENTEGEYSSLEH 178
Cdd:PRK07006  84 LIREYRVAIKGPLTT-----PvggGIRSLNVALRQELDLYVclrPVRYFKGVPSPVKRPEDTDMVIFRENSEDIYAGIEW 158

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 179 EsvAGVVESLKII-------------------------TKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQ 233
Cdd:PRK07006 159 K--AGSAEAKKVIkflqeemgvkkirfpetsgigikpvSEEGTERLVRAAIEYAIDNDRKSVTLVHKGNIMKFTEGAFKD 236

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 234 CCKEVAARY--------------------PQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLV 293
Cdd:PRK07006 237 WGYQLAEEEfgdelidggpwdkiknpetgKEIIVKDSIADAFLQQILLRPAEYDVIATMNLNGDYISDALAAQVGGIGIA 316

                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 585177563 294 AGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAV 351
Cdd:PRK07006 317 PGANINDGHAIFE-ATHGTAPKYAGLDKVNPGSVILSAEMMLRHMGWTEAADLIIKSM 373
PLN02329 PLN02329
3-isopropylmalate dehydrogenase
 53-369 6.01e-26

3-isopropylmalate dehydrogenase


Pssm-ID: 215188  Cd Length: 409  Bit Score: 108.24  E-value: 6.01e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563  53 RHTVTMIPGDGIGPELMLHVKSVFRHAC----VPVDFEEVHVSSNA-DEEDI---RNAIMAIRRNRVALKGNI------E 118
Cdd:PLN02329  46 RYNIALLPGDGIGPEVISVAKNVLQKAGslegLEFDFQEMPVGGAAlDLVGVplpEETFTAAKQSDAILLGAIggykwdK 125

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 119 TNHNLPPSHKSRNniLRTSLDLYANVIHCKSLPGVVTRH-------RDIDILIVRENTEGEYSSLE-----HESVAGVVE 186
Cdd:PLN02329 126 NEKHLRPEMALFY--LRRDLKVFANLRPATVLPQLVDAStlkkevaEGVDMMIVRELTGGIYFGEPrgitiNENGEEVGV 203

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 187 SLKIITKAKSLRIAEYAFKLAQESgRKKVTAVHKANIMKlGDGLFLQCCKEVAARYPQITFENMIVDNTTMQLVSRPQQF 266
Cdd:PLN02329 204 STEIYAAHEIDRIARVAFETARKR-RGKLCSVDKANVLD-ASILWRKRVTALASEYPDVELSHMYVDNAAMQLIRDPKQF 281

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 267 DVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDH-LKLHSYAT 345
Cdd:PLN02329 282 DTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYgLGEEKAAK 361

                        330       340
                 ....*....|....*....|....
gi 585177563 346 SIRKAVLASMdNENMHTPDIGGQG 369
Cdd:PLN02329 362 RIEDAVVDAL-NKGFRTGDIYSPG 384
PRK07362 PRK07362
NADP-dependent isocitrate dehydrogenase;
103-357 2.84e-21

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 180944  Cd Length: 474  Bit Score: 95.17  E-value: 2.84e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 103 IMAIRRNRVALKGNIETnhnlpP---SHKSRNNILRTSLDLYANVIHCKSLPGVVTRHR---DIDILIVRENTEGEYSSL 176
Cdd:PRK07362  91 LEAIREYGVAIKGPLTT-----PiggGIRSLNVALRQIFDLYSCVRPCRYYAGTPSPHKnpeKLDVIVYRENTEDIYMGI 165

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 177 EHESVAGVVESL----------------------------KIITKAKSLRIAEYAFK--LAQESGRKKVTAVHKANIMKL 226
Cdd:PRK07362 166 EWEAGDEIGDKLikhlneevipaspelgkrqiplgsgigiKPVSKTGSQRHIRRAIEhaLRLPGDKRHVTLVHKGNIMKY 245

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 227 GDGLFLQCCKEVAA--------------------RYPQITFEN------------------------------------- 249
Cdd:PRK07362 246 TEGAFRDWGYELATtefrdecvtereswilsnkeKNPNISIEDnarmiepgydsltpekkaaicaevkevldsiwsshgn 325

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 585177563 250 -----------MIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFEtATRNTGKSIAN 318
Cdd:PRK07362 326 gkwkekvlvddRIADSIFQQIQTRPQEYSILATLNLNGDYISDAAAAIVGGLGMAPGANIGDNAAIFE-ATHGTAPKHAG 404

                        330       340       350
                 ....*....|....*....|....*....|....*....
gi 585177563 319 KNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDN 357
Cdd:PRK07362 405 LDRINPGSVILSGVMMLEYLGWQEAADLITKGLSAAIAN 443
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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