Cap-0 specific (nucleoside-2'-O-)-methyltransferase of orbivirus; Cap-0 specific (nucleoside-2 ...
178-388
5.76e-121
Cap-0 specific (nucleoside-2'-O-)-methyltransferase of orbivirus; Cap-0 specific (nucleoside-2'-O-)-methyltransferase (2'OMTase) catalyzes the methylation of Cap-0 (m7GpppNp) at the 2'-hydroxyl of the ribose of the first nucleotide, using S-adenosyl-L-methionine (AdoMet) as the methyl donor. This reaction is the fourth and last step in mRNA capping, the creation of the stabilizing five-prime cap (5' cap) on mRNA. Orbiviruses, which comprise a family of dsRNA viruses, cap their mRNAs. The 2'OMTase activity is located in the multifunctional capping enzyme, VP4.
:
Pssm-ID: 467733 Cd Length: 211 Bit Score: 358.00 E-value: 5.76e-121
Orbivirus VP4 core protein; Orbiviruses are double stranded RNA retroviruses of which the ...
1-624
3.04e-115
Orbivirus VP4 core protein; Orbiviruses are double stranded RNA retroviruses of which the bluetongue virus is a member. The core of bluetongue virus (BTV) is a multienzyme complex composed of two major proteins (VP7 and VP3) and three minor proteins (VP1, VP4 and VP6) in addition to the viral genome. VP4 has been shown to perform all RNA capping activities and has both methyltransferase type 1 and type 2 activities associated with it.
The actual alignment was detected with superfamily member pfam05059:
Pssm-ID: 461538 Cd Length: 640 Bit Score: 358.63 E-value: 3.04e-115
Cap-0 specific (nucleoside-2'-O-)-methyltransferase of orbivirus; Cap-0 specific (nucleoside-2 ...
178-388
5.76e-121
Cap-0 specific (nucleoside-2'-O-)-methyltransferase of orbivirus; Cap-0 specific (nucleoside-2'-O-)-methyltransferase (2'OMTase) catalyzes the methylation of Cap-0 (m7GpppNp) at the 2'-hydroxyl of the ribose of the first nucleotide, using S-adenosyl-L-methionine (AdoMet) as the methyl donor. This reaction is the fourth and last step in mRNA capping, the creation of the stabilizing five-prime cap (5' cap) on mRNA. Orbiviruses, which comprise a family of dsRNA viruses, cap their mRNAs. The 2'OMTase activity is located in the multifunctional capping enzyme, VP4.
Pssm-ID: 467733 Cd Length: 211 Bit Score: 358.00 E-value: 5.76e-121
Orbivirus VP4 core protein; Orbiviruses are double stranded RNA retroviruses of which the ...
1-624
3.04e-115
Orbivirus VP4 core protein; Orbiviruses are double stranded RNA retroviruses of which the bluetongue virus is a member. The core of bluetongue virus (BTV) is a multienzyme complex composed of two major proteins (VP7 and VP3) and three minor proteins (VP1, VP4 and VP6) in addition to the viral genome. VP4 has been shown to perform all RNA capping activities and has both methyltransferase type 1 and type 2 activities associated with it.
Pssm-ID: 461538 Cd Length: 640 Bit Score: 358.63 E-value: 3.04e-115
Cap-0 specific (nucleoside-2'-O-)-methyltransferase of orbivirus; Cap-0 specific (nucleoside-2 ...
178-388
5.76e-121
Cap-0 specific (nucleoside-2'-O-)-methyltransferase of orbivirus; Cap-0 specific (nucleoside-2'-O-)-methyltransferase (2'OMTase) catalyzes the methylation of Cap-0 (m7GpppNp) at the 2'-hydroxyl of the ribose of the first nucleotide, using S-adenosyl-L-methionine (AdoMet) as the methyl donor. This reaction is the fourth and last step in mRNA capping, the creation of the stabilizing five-prime cap (5' cap) on mRNA. Orbiviruses, which comprise a family of dsRNA viruses, cap their mRNAs. The 2'OMTase activity is located in the multifunctional capping enzyme, VP4.
Pssm-ID: 467733 Cd Length: 211 Bit Score: 358.00 E-value: 5.76e-121
Orbivirus VP4 core protein; Orbiviruses are double stranded RNA retroviruses of which the ...
1-624
3.04e-115
Orbivirus VP4 core protein; Orbiviruses are double stranded RNA retroviruses of which the bluetongue virus is a member. The core of bluetongue virus (BTV) is a multienzyme complex composed of two major proteins (VP7 and VP3) and three minor proteins (VP1, VP4 and VP6) in addition to the viral genome. VP4 has been shown to perform all RNA capping activities and has both methyltransferase type 1 and type 2 activities associated with it.
Pssm-ID: 461538 Cd Length: 640 Bit Score: 358.63 E-value: 3.04e-115
viral Cap-0 specific (nucleoside-2'-O-)-methyltransferase; Cap-0 specific (nucleoside-2'-O-) ...
178-355
1.22e-14
viral Cap-0 specific (nucleoside-2'-O-)-methyltransferase; Cap-0 specific (nucleoside-2'-O-)-methyltransferase (2'OMTase) catalyzes the methylation of Cap-0 (m7GpppNp) at the 2'-hydroxyl of the ribose of the first nucleotide, using S-adenosyl-L-methionine (AdoMet) as the methyl donor. This reaction is the fourth and last step in mRNA capping, the creation of the stabilizing five-prime cap (5' cap) on mRNA. Some dsDNA and dsRNA viruses, like the bluetongue virus (BTV), a member of the Reoviridae family, and Vaccinia virus, a member of the Poxviridae family, as well as some ss(+)RNA viruses, like Flaviviridae and Nidovirales, cap their mRNAs and encode their own 2'OMTase. In BTV, all four reactions are catalyzed by a single protein, VP4. In Vaccinia, the activity is located in the processing factor of the poly(A) polymerase, VP39.
Pssm-ID: 467730 Cd Length: 179 Bit Score: 72.47 E-value: 1.22e-14
Database: CDSEARCH/cdd Low complexity filter: no Composition Based Adjustment: yes E-value threshold: 0.01
References:
Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
of the residues that compose this conserved feature have been mapped to the query sequence.
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The table lists conserved domains identified on the query sequence. Click on the plus sign (+) on the left to display full descriptions, alignments, and scores.
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(labeled illustration) Standard Display shows only the best scoring domain model from each source, in each hit category listed below for each region on the query sequence.
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