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Conserved domains on  [gi|532373745|gb|EQL82305|]
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acetyltransferase (GNAT) domain protein [Streptococcus pyogenes UTSW-2]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 10456837)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  15581578|10940244|9175471|27367672|26818562
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PhnO super family cl42766
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 39-148 9.69e-14

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


The actual alignment was detected with superfamily member COG0454:

Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 64.30  E-value: 9.69e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 532373745  39 LRRIPSINPD-LLTIETS-------YLEKGDCFWLAVNEESRVIGSIAYSSIPKtNEVFLHRLFIKPSLKHQGIGSQLLT 110
Cdd:COG0454    3 IRKATPEDINfILLIEALdaelkamEGSLAGAEFIAVDDKGEPIGFAGLRRLDD-KVLELKRLYVLPEYRGKGIGKALLE 81

                         90       100       110
                 ....*....|....*....|....*....|....*...
gi 532373745 111 FAEDYLRKIGKSHVRIHLGQPKEqwfESYRFYPKHGYQ 148
Cdd:COG0454   82 ALLEWARERGCTALELDTLDGNP---AAIRFYERLGFK 116
 
Name Accession Description Interval E-value
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 39-148 9.69e-14

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 64.30  E-value: 9.69e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 532373745  39 LRRIPSINPD-LLTIETS-------YLEKGDCFWLAVNEESRVIGSIAYSSIPKtNEVFLHRLFIKPSLKHQGIGSQLLT 110
Cdd:COG0454    3 IRKATPEDINfILLIEALdaelkamEGSLAGAEFIAVDDKGEPIGFAGLRRLDD-KVLELKRLYVLPEYRGKGIGKALLE 81

                         90       100       110
                 ....*....|....*....|....*....|....*...
gi 532373745 111 FAEDYLRKIGKSHVRIHLGQPKEqwfESYRFYPKHGYQ 148
Cdd:COG0454   82 ALLEWARERGCTALELDTLDGNP---AAIRFYERLGFK 116
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 46-147 6.54e-11

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 56.37  E-value: 6.54e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 532373745   46 NPDLLTIETSYLEKGDCFWLAVNEESRVIGSIAYSSIPKT-NEVFLHRLFIKPSLKHQGIGSQLLTFAEDYLRKIGKSHV 124
Cdd:pfam00583  17 PDEPLDLLEDWDEDASEGFFVAEEDGELVGFASLSIIDDEpPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERI 96

                          90       100
                  ....*....|....*....|...
gi 532373745  125 RIHLGQPKEQwfeSYRFYPKHGY 147
Cdd:pfam00583  97 FLEVAADNLA---AIALYEKLGF 116
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 63-127 1.63e-09

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 51.12  E-value: 1.63e-09
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 532373745  63 FWLAVNEEsRVIGSIAYSSIPKT-NEVFLHRLFIKPSLKHQGIGSQLLTFAEDYLRKIGKSHVRIH 127
Cdd:cd04301    1 FLVAEDDG-EIVGFASLSPDGSGgDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
 
Name Accession Description Interval E-value
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 39-148 9.69e-14

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 64.30  E-value: 9.69e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 532373745  39 LRRIPSINPD-LLTIETS-------YLEKGDCFWLAVNEESRVIGSIAYSSIPKtNEVFLHRLFIKPSLKHQGIGSQLLT 110
Cdd:COG0454    3 IRKATPEDINfILLIEALdaelkamEGSLAGAEFIAVDDKGEPIGFAGLRRLDD-KVLELKRLYVLPEYRGKGIGKALLE 81

                         90       100       110
                 ....*....|....*....|....*....|....*...
gi 532373745 111 FAEDYLRKIGKSHVRIHLGQPKEqwfESYRFYPKHGYQ 148
Cdd:COG0454   82 ALLEWARERGCTALELDTLDGNP---AAIRFYERLGFK 116
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
61-148 8.28e-13

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 62.03  E-value: 8.28e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 532373745  61 DCFWLAVnEESRVIGSIAYSSIPKTNE---VFLHRLFIKPSLKHQGIGSQLLTFAEDYLRKIGKSHVRIHlGQPkeqwfE 137
Cdd:COG3153   39 GLSLVAE-DDGEIVGHVALSPVDIDGEgpaLLLGPLAVDPEYRGQGIGRALMRAALEAARERGARAVVLL-GDP-----S 111

                         90
                 ....*....|.
gi 532373745 138 SYRFYPKHGYQ 148
Cdd:COG3153  112 LLPFYERFGFR 122
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 56-155 5.50e-11

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 56.92  E-value: 5.50e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 532373745  56 YLEKGDCFWLAVnEESRVIGSIAYSSIPkTNEVFLHRLFIKPSLKHQGIGSQLLTFAEDYLRKIGKSHVRIHLGQPkeqw 135
Cdd:COG1246   23 LEEEIGEFWVAE-EDGEIVGCAALHPLD-EDLAELRSLAVHPDYRGRGIGRRLLEALLAEARELGLKRLFLLTTSA---- 96

                         90       100
                 ....*....|....*....|
gi 532373745 136 feSYRFYPKHGYQFYDDTHL 155
Cdd:COG1246   97 --AIHFYEKLGFEEIDKEDL 114
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 46-147 6.54e-11

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 56.37  E-value: 6.54e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 532373745   46 NPDLLTIETSYLEKGDCFWLAVNEESRVIGSIAYSSIPKT-NEVFLHRLFIKPSLKHQGIGSQLLTFAEDYLRKIGKSHV 124
Cdd:pfam00583  17 PDEPLDLLEDWDEDASEGFFVAEEDGELVGFASLSIIDDEpPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERI 96

                          90       100
                  ....*....|....*....|...
gi 532373745  125 RIHLGQPKEQwfeSYRFYPKHGY 147
Cdd:pfam00583  97 FLEVAADNLA---AIALYEKLGF 116
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 59-148 5.30e-10

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 52.84  E-value: 5.30e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 532373745   59 KGDCFWLAVNEEsRVIGSIAYSSIPKTNEVFLHRLFIKPSLKHQGIGSQLLTFAEDYLRKIGKSHVRIHLGQPKEqwfes 138
Cdd:pfam13508   1 PGGRFFVAEDDG-KIVGFAALLPLDDEGALAELRLAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLELETTNRAA----- 74

                          90
                  ....*....|
gi 532373745  139 yRFYPKHGYQ 148
Cdd:pfam13508  75 -AFYEKLGFE 83
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 63-127 1.63e-09

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 51.12  E-value: 1.63e-09
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 532373745  63 FWLAVNEEsRVIGSIAYSSIPKT-NEVFLHRLFIKPSLKHQGIGSQLLTFAEDYLRKIGKSHVRIH 127
Cdd:cd04301    1 FLVAEDDG-EIVGFASLSPDGSGgDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 75-153 3.64e-09

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 51.19  E-value: 3.64e-09
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 532373745  75 GSIAYSSIPKTNEVFLHRLFIKPSLKHQGIGSQLLTFAEDYLRKIGKSHVRIHLGQPKEQwfeSYRFYPKHGYQFYDDT 153
Cdd:COG0456    1 GFALLGLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEA---AIALYEKLGFEEVGER 76
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 56-148 8.54e-07

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 45.34  E-value: 8.54e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 532373745   56 YLEKGDCFWLAVNEESRVIGSIAYSsipktNEVFLHRLFIKPSLKHQGIGSQLLTFAEDYLRK--IGKSHVRIHlGQPke 133
Cdd:pfam13673  25 RIDQGEYFFFVAFEGGQIVGVIALR-----DRGHISLLFVDPDYQGQGIGKALLEAVEDYAEKdgIKLSELTVN-ASP-- 96

                          90
                  ....*....|....*
gi 532373745  134 qwfESYRFYPKHGYQ 148
Cdd:pfam13673  97 ---YAVPFYEKLGFR 108
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
63-148 3.41e-06

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 44.60  E-value: 3.41e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 532373745  63 FWLAVNEESRVIGSIA---YSSIPKTNEVFLHRLFIKPSLKHQGIGSQLLTFAEDYLRKIGKSHVR--IHLGQPKeqwfe 137
Cdd:COG1247   53 PVLVAEEDGEVVGFASlgpFRPRPAYRGTAEESIYVDPDARGRGIGRALLEALIERARARGYRRLVavVLADNEA----- 127

                         90
                 ....*....|.
gi 532373745 138 SYRFYPKHGYQ 148
Cdd:COG1247  128 SIALYEKLGFE 138
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
77-155 4.46e-06

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 43.63  E-value: 4.46e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 532373745  77 IAYSSI--PKTNEVFLHRLFIKPSLKHQGIGSQLLTFAEDYLRKIGKSHVRIHlGQPkeqwfESYRFYPKHGYQFYDDTH 154
Cdd:COG2153   46 VATARLlpPGDGEAKIGRVAVLPEYRGQGLGRALMEAAIEEARERGARRIVLS-AQA-----HAVGFYEKLGFVPVGEEF 119


                 .
gi 532373745 155 L 155
Cdd:COG2153  120 L 120
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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