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Conserved domains on  [gi|530406470|ref|XP_005254734|]
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sorting nexin-22 isoform X1 [Homo sapiens]

Protein Classification

PX domain-containing protein( domain architecture ID 572)

PX (Phox Homology) domain-containing protein may bind phosphoinositides and may function in targeting proteins to membranes

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PX_domain super family cl02563
The Phox Homology domain, a phosphoinositide binding module; The PX domain is a ...
 2-88 2.19e-32

The Phox Homology domain, a phosphoinositide binding module; The PX domain is a phosphoinositide (PI) binding module involved in targeting proteins to membranes. Proteins containing PX domains interact with PIs and have been implicated in highly diverse functions such as cell signaling, vesicular trafficking, protein sorting, lipid modification, cell polarity and division, activation of T and B cells, and cell survival. Many members of this superfamily bind phosphatidylinositol-3-phosphate (PI3P) but in some cases, other PIs such as PI4P or PI(3,4)P2, among others, are the preferred substrates. In addition to protein-lipid interaction, the PX domain may also be involved in protein-protein interaction, as in the cases of p40phox, p47phox, and some sorting nexins (SNXs). The PX domain is conserved from yeast to humans and is found in more than 100 proteins. The majority of PX domain-containing proteins are SNXs, which play important roles in endosomal sorting.


The actual alignment was detected with superfamily member cd06880:

Pssm-ID: 470617  Cd Length: 110  Bit Score: 111.98  E-value: 2.19e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530406470   2 LEVHIPSVGPEAE----------------GPRQSPEKSH--------MIKKLYKVPDFPSKRLPNWRTRGLEQRRQGLEA 57
Cdd:cd06880    1 IEVSIPSYRLEVDesekpytvftievlvnGRRHTVEKRYsefhalhkKLKKSIKTPDFPPKRVRNWNPKVLEQRRQGLEA 80

                         90       100       110
                 ....*....|....*....|....*....|.
gi 530406470  58 YIQGILYLNqEVPKELLEFLRLRHFPTDPKA 88
Cdd:cd06880   81 YLQGLLKIN-ELPKQLLDFLGVRHFPSLPKS 110
 
Name Accession Description Interval E-value
PX_SNX22 cd06880
The phosphoinositide binding Phox Homology domain of Sorting Nexin 22; The PX domain is a ...
 2-88 2.19e-32

The phosphoinositide binding Phox Homology domain of Sorting Nexin 22; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX22 may be involved in recruiting other proteins to the membrane via protein-protein and protein-ligand interaction. The biological function of SNX22 is not yet known.


Pssm-ID: 132790  Cd Length: 110  Bit Score: 111.98  E-value: 2.19e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530406470   2 LEVHIPSVGPEAE----------------GPRQSPEKSH--------MIKKLYKVPDFPSKRLPNWRTRGLEQRRQGLEA 57
Cdd:cd06880    1 IEVSIPSYRLEVDesekpytvftievlvnGRRHTVEKRYsefhalhkKLKKSIKTPDFPPKRVRNWNPKVLEQRRQGLEA 80

                         90       100       110
                 ....*....|....*....|....*....|.
gi 530406470  58 YIQGILYLNqEVPKELLEFLRLRHFPTDPKA 88
Cdd:cd06880   81 YLQGLLKIN-ELPKQLLDFLGVRHFPSLPKS 110
PX pfam00787
PX domain; PX domains bind to phosphoinositides.
 24-77 3.57e-03

PX domain; PX domains bind to phosphoinositides.


Pssm-ID: 459940  Cd Length: 84  Bit Score: 34.91  E-value: 3.57e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 530406470   24 HMIKKLYK---VPDFPSKRLP-NWRTRGLEQRRQGLEAYIQGILyLNQEVPK--ELLEFL 77
Cdd:pfam00787  23 KKLLRKFPsviIPPLPPKRWLgRYNEEFIEKRRKGLEQYLQRLL-QHPELRNseVLLEFL 81
PX smart00312
PhoX homologous domain, present in p47phox and p40phox; Eukaryotic domain of unknown function ...
 28-77 9.76e-03

PhoX homologous domain, present in p47phox and p40phox; Eukaryotic domain of unknown function present in phox proteins, PLD isoforms, a PI3K isoform.


Pssm-ID: 214610  Cd Length: 105  Bit Score: 34.24  E-value: 9.76e-03
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*.
gi 530406470    28 KLYKVPDFPSK----RLPNWRTRGLEQRRQGLEAYIQGILYLNQEVP--KELLEFL 77
Cdd:smart00312  49 PRSILPPLPGKklfgRLNNFSEEFIEKRRRGLEKYLQSLLNHPELINhsEVVLEFL 104
 
Name Accession Description Interval E-value
PX_SNX22 cd06880
The phosphoinositide binding Phox Homology domain of Sorting Nexin 22; The PX domain is a ...
 2-88 2.19e-32

The phosphoinositide binding Phox Homology domain of Sorting Nexin 22; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX22 may be involved in recruiting other proteins to the membrane via protein-protein and protein-ligand interaction. The biological function of SNX22 is not yet known.


Pssm-ID: 132790  Cd Length: 110  Bit Score: 111.98  E-value: 2.19e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530406470   2 LEVHIPSVGPEAE----------------GPRQSPEKSH--------MIKKLYKVPDFPSKRLPNWRTRGLEQRRQGLEA 57
Cdd:cd06880    1 IEVSIPSYRLEVDesekpytvftievlvnGRRHTVEKRYsefhalhkKLKKSIKTPDFPPKRVRNWNPKVLEQRRQGLEA 80

                         90       100       110
                 ....*....|....*....|....*....|.
gi 530406470  58 YIQGILYLNqEVPKELLEFLRLRHFPTDPKA 88
Cdd:cd06880   81 YLQGLLKIN-ELPKQLLDFLGVRHFPSLPKS 110
PX_domain cd06093
The Phox Homology domain, a phosphoinositide binding module; The PX domain is a ...
 26-79 6.25e-05

The Phox Homology domain, a phosphoinositide binding module; The PX domain is a phosphoinositide (PI) binding module involved in targeting proteins to membranes. Proteins containing PX domains interact with PIs and have been implicated in highly diverse functions such as cell signaling, vesicular trafficking, protein sorting, lipid modification, cell polarity and division, activation of T and B cells, and cell survival. Many members of this superfamily bind phosphatidylinositol-3-phosphate (PI3P) but in some cases, other PIs such as PI4P or PI(3,4)P2, among others, are the preferred substrates. In addition to protein-lipid interaction, the PX domain may also be involved in protein-protein interaction, as in the cases of p40phox, p47phox, and some sorting nexins (SNXs). The PX domain is conserved from yeast to humans and is found in more than 100 proteins. The majority of PX domain-containing proteins are SNXs, which play important roles in endosomal sorting.


Pssm-ID: 132768 [Multi-domain]  Cd Length: 106  Bit Score: 40.42  E-value: 6.25e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 530406470  26 IKKLYK---VPDFPSKRLP-NWRTRGLEQRRQGLEAYIQGILYLNQEV-PKELLEFLRL 79
Cdd:cd06093   48 LKKKFPgviLPPLPPKKLFgNLDPEFIEERRKQLEQYLQSLLNHPELRnSEELKEFLEL 106
PX_IRAS cd06875
The phosphoinositide binding Phox Homology domain of the Imidazoline Receptor ...
 48-82 1.72e-04

The phosphoinositide binding Phox Homology domain of the Imidazoline Receptor Antisera-Selected; The PX domain is a phosphoinositide binding (PI) module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. Imidazoline Receptor Antisera-Selected (IRAS), also called nischarin, contains an N-terminal PX domain, leucine rich repeats, and a predicted coiled coil domain. The PX domain of IRAS binds to phosphatidylinositol-3-phosphate in membranes. Together with the coiled coil domain, it is essential for the localization of IRAS to endosomes. IRAS has been shown to interact with integrin and inhibit cell migration. Its interaction with alpha5 integrin causes a redistribution of the receptor from the cell surface to endosomal structures, suggesting that IRAS may function as a sorting nexin (SNX) which regulates the endosomal trafficking of integrin. SNXs make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway.


Pssm-ID: 132785  Cd Length: 116  Bit Score: 39.19  E-value: 1.72e-04
                         10        20        30
                 ....*....|....*....|....*....|....*.
gi 530406470  48 LEQRRQGLEAYIQGIL-YLNQEVPKELLEFLRLRHF 82
Cdd:cd06875   72 VEKRRKELEIYLQTLLsFFQKTMPRELAHFLDFHKY 107
PX_SNX17_31 cd06885
The phosphoinositide binding Phox Homology domain of Sorting Nexins 17 and 31; The PX domain ...
 26-62 2.11e-04

The phosphoinositide binding Phox Homology domain of Sorting Nexins 17 and 31; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Members of this subfamily include sorting nexin 17 (SNX17), SNX31, and similar proteins. They contain an N-terminal PX domain followed by a truncated FERM (4.1, ezrin, radixin, and moesin) domain and a unique C-terminal region. SNXs make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX17 is known to regulate the trafficking and processing of a number of proteins. It binds some members of the low-density lipoprotein receptor (LDLR) family such as LDLR, VLDLR, ApoER2, and others, regulating their endocytosis. It also binds P-selectin and may regulate its lysosomal degradation. SNX17 is highly expressed in neurons. It binds amyloid precursor protein (APP) and may be involved in its intracellular trafficking and processing to amyloid beta peptide, which plays a central role in the pathogenesis of Alzheimer's disease. The biological function of SNX31 is unknown.


Pssm-ID: 132795  Cd Length: 104  Bit Score: 38.85  E-value: 2.11e-04
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|
gi 530406470  26 IKKLY---KVPDFPSKRLPNWRTRGLEQRRQGLEAYIQGI 62
Cdd:cd06885   45 LKKEFgnrKLPPFPPKKLLPLTPAQLEERRLQLEKYLQAV 84
PX pfam00787
PX domain; PX domains bind to phosphoinositides.
 24-77 3.57e-03

PX domain; PX domains bind to phosphoinositides.


Pssm-ID: 459940  Cd Length: 84  Bit Score: 34.91  E-value: 3.57e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 530406470   24 HMIKKLYK---VPDFPSKRLP-NWRTRGLEQRRQGLEAYIQGILyLNQEVPK--ELLEFL 77
Cdd:pfam00787  23 KKLLRKFPsviIPPLPPKRWLgRYNEEFIEKRRKGLEQYLQRLL-QHPELRNseVLLEFL 81
PX smart00312
PhoX homologous domain, present in p47phox and p40phox; Eukaryotic domain of unknown function ...
 28-77 9.76e-03

PhoX homologous domain, present in p47phox and p40phox; Eukaryotic domain of unknown function present in phox proteins, PLD isoforms, a PI3K isoform.


Pssm-ID: 214610  Cd Length: 105  Bit Score: 34.24  E-value: 9.76e-03
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*.
gi 530406470    28 KLYKVPDFPSK----RLPNWRTRGLEQRRQGLEAYIQGILYLNQEVP--KELLEFL 77
Cdd:smart00312  49 PRSILPPLPGKklfgRLNNFSEEFIEKRRRGLEKYLQSLLNHPELINhsEVVLEFL 104
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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