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Conserved domains on  [gi|524105081|emb|CCY80752|]
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universal bacterial protein YeaZ [Prevotella sp. CAG:1185]

Protein Classification

tRNA threonylcarbamoyladenosine biosynthesis protein TsaB( domain architecture ID 10003119)

tRNA threonylcarbamoyladenosine biosynthesis protein TsaB (also known as YeaZ) is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine

EC:  2.3.1.234
Gene Ontology:  GO:0002949
PubMed:  22378793

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TsaB COG1214
tRNA A37 threonylcarbamoyladenosine modification protein TsaB [Translation, ribosomal ...
 27-248 1.34e-75

tRNA A37 threonylcarbamoyladenosine modification protein TsaB [Translation, ribosomal structure and biogenesis]; tRNA A37 threonylcarbamoyladenosine modification protein TsaB is part of the Pathway/BioSystem: tRNA modification


:

Pssm-ID: 440827  Cd Length: 227  Bit Score: 228.58  E-value: 1.34e-75
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081  27 ILNIETSTNVCSVAVSQDGACIFSKEDHSGPNHAVKLGIFVDEA-----ISFADshaipLDAVAVSCGPGSYTGLRIGVS 101
Cdd:COG1214    3 ILAIDTSTEACSVALLDDGEVLAEREENDGRGHSERLLPMIDELlaeagLTLSD-----LDAIAVGIGPGSFTGLRIGVA 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081 102 MAKGICYGRNIKLIAVPTLELLTVPVLlthePEPGALLCPMLDARRMEVYSAIYDRSLKEVRGVQAD-VVDADTYKEFLD 180
Cdd:COG1214   78 TAKGLALALGIPLVGVSSLEALAAQAP----RAGAGLVLVAIDARRGEVYWAVYDADDGELERLGEPrVLAPEELAEPLA 153

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 524105081 181 KGKVYFFGNGACKCIEAINHPNAVLLKGI-EPLAKNMFPLAEKRMALENFEDVAYFVPFYLKDFVAKTP 248
Cdd:COG1214  154 GGPVLFVGDGAEAYAELLAEALAVLADALaLPSAAALARLAAARLAAGEAEDPADLEPLYLRDPDAEPP 222
 
Name Accession Description Interval E-value
TsaB COG1214
tRNA A37 threonylcarbamoyladenosine modification protein TsaB [Translation, ribosomal ...
 27-248 1.34e-75

tRNA A37 threonylcarbamoyladenosine modification protein TsaB [Translation, ribosomal structure and biogenesis]; tRNA A37 threonylcarbamoyladenosine modification protein TsaB is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440827  Cd Length: 227  Bit Score: 228.58  E-value: 1.34e-75
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081  27 ILNIETSTNVCSVAVSQDGACIFSKEDHSGPNHAVKLGIFVDEA-----ISFADshaipLDAVAVSCGPGSYTGLRIGVS 101
Cdd:COG1214    3 ILAIDTSTEACSVALLDDGEVLAEREENDGRGHSERLLPMIDELlaeagLTLSD-----LDAIAVGIGPGSFTGLRIGVA 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081 102 MAKGICYGRNIKLIAVPTLELLTVPVLlthePEPGALLCPMLDARRMEVYSAIYDRSLKEVRGVQAD-VVDADTYKEFLD 180
Cdd:COG1214   78 TAKGLALALGIPLVGVSSLEALAAQAP----RAGAGLVLVAIDARRGEVYWAVYDADDGELERLGEPrVLAPEELAEPLA 153

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 524105081 181 KGKVYFFGNGACKCIEAINHPNAVLLKGI-EPLAKNMFPLAEKRMALENFEDVAYFVPFYLKDFVAKTP 248
Cdd:COG1214  154 GGPVLFVGDGAEAYAELLAEALAVLADALaLPSAAALARLAAARLAAGEAEDPADLEPLYLRDPDAEPP 222
ASKHA_NBD_TsaB cd24032
nucleotide-binding domain (NBD) of tRNA threonylcarbamoyladenosine biosynthesis protein TsaB ...
 27-233 8.67e-54

nucleotide-binding domain (NBD) of tRNA threonylcarbamoyladenosine biosynthesis protein TsaB (previously known as YeaZ) and similar proteins; TsaB, also called t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaB, is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. It may be involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaE. TsaB seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. In fact, it can act as a protease that specifically degrades TsaD in vitro; therefore, TsaB may post-translationally regulate cellular pools of TsaD via proteolytic degradation. TsaB does not show sialoglycoprotease activity against glycophorin A.


Pssm-ID: 466882 [Multi-domain]  Cd Length: 205  Bit Score: 172.46  E-value: 8.67e-54
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081  27 ILNIETSTNVCSVAVSQDGACIFSKEDHSGPNHAVKLGIFVDEA-----ISFADshaipLDAVAVSCGPGSYTGLRIGVS 101
Cdd:cd24032    1 ILAIDTSTSACSVALLKGGKILAEYELDLGRRHSERLLPMIDELlkeagLSLKD-----LDAIAVGIGPGSFTGLRIGLA 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081 102 MAKGICYGRNIKLIAVPTLELLTvpvllTHEPEPGALLCPMLDARRMEVYSAIYDRSLKEVRGVQADVV--DADTYKEFL 179
Cdd:cd24032   76 TAKGLALALGIPLVGVSTLEALA-----QNAPRADGRVLPAIDARRGEVYWALYERDKGGLILEEEEAVlpPEELEEILL 150

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....
gi 524105081 180 DKGKVYFFGNGACKCIEAINHPNAVLLKGIEPLAKNMFPLAEKRMALENFEDVA 233
Cdd:cd24032  151 LKKPAILVGDGWDKYPDLIKENPVLPIELLLPSAADLAPLALAKLAKGETDDEE 204
T6A_YeaZ TIGR03725
tRNA threonylcarbamoyl adenosine modification protein YeaZ; This family describes a protein ...
 27-241 1.18e-52

tRNA threonylcarbamoyl adenosine modification protein YeaZ; This family describes a protein family, YeaZ, now associated with the threonylcarbamoyl adenosine (t6A) tRNA modification. Members of this family may occur as fusions with ygjD (previously gcp) or the ribosomal protein N-acetyltransferase rimI, and is frequently encoded next to rimI. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 274750 [Multi-domain]  Cd Length: 204  Bit Score: 169.37  E-value: 1.18e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081   27 ILNIETSTNVCSVAVSQDGACIFSKEDHSGPNHAVKLGIFVDEA-----ISFADshaipLDAVAVSCGPGSYTGLRIGVS 101
Cdd:TIGR03725   1 ILAIDTSTEALSVALLDDGKVLAERTEPAGRNHSERLLPMIEELlaeagLSLQD-----LDAIAVGVGPGSFTGLRIGLA 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081  102 MAKGICYGRNIKLIAVPTLELLTVPVLLTHEPEPgalLCPMLDARRMEVYSAIYDrsLKEVRGVQadVVDADTYKEFLDK 181
Cdd:TIGR03725  76 TAKGLALALGIPLVGVSSLEALAAQAAAQDGGGP---VLVAIDARRGEVYWGLYD--LKPLEEPA--VLSPEELLELLKE 148

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081  182 GKVYFFGNGACKCIEAINHPNAVLLkgiePLAKNMFPLAEKRMALENFEDVAYFVPFYLK 241
Cdd:TIGR03725 149 LNVLLVGDGAEAYALALLAAAADAL----PDAAALARLALARLKAGEPLDVEELEPLYLR 204
TsaD pfam00814
tRNA N6-adenosine threonylcarbamoyltransferase; This domain can be found in Kae1/Qri7/YgjD, ...
 58-153 1.35e-25

tRNA N6-adenosine threonylcarbamoyltransferase; This domain can be found in Kae1/Qri7/YgjD, products of COG0533 that belong to a small group of 60 proteins that are present in all three domains of life. COG0533 proteins are suggest to play a role in a post translational modification of certain tRNAs. For example, YgjD along with YeaZ, YjeE, and YrdC have been deemed necessary and sufficient for the tRNA modification. This modification involves the formation of N6-threonyl carbamoyl adenosine (t6A) at position 37 in the anti-codon stem loop which is critical for translational speed and accuracy. Structural analysis indicate that YeaZ lacks resemblance to any known protease active site. Together with the absence of a putative zinc-binding motif. Thus the likelyhood of it being a protease, as previously thought, has been negated. EC:2.3.1.234


Pssm-ID: 395656  Cd Length: 272  Bit Score: 101.31  E-value: 1.35e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081   58 NHAVKLGIFVDEAISFADSHAIPLDAVAVSCGPGSYTGLRIGVSMAKGICYGRNIKLIAVPTLELLTVPVLLTHEPEPga 137
Cdd:pfam00814  28 HHSERLMPLIDEALAEAGLSLEDLDAIAVTKGPGLFTGLRVGASFAKGLALALNKPLVGVNHLEAHALAARLETGLEF-- 105

                          90
                  ....*....|....*.
gi 524105081  138 LLCPMLDARRMEVYSA 153
Cdd:pfam00814 106 PVVLLVSGGHTQVYAA 121
PRK09604 PRK09604
tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD;
27-139 2.23e-10

tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD;


Pssm-ID: 236585  Cd Length: 332  Bit Score: 59.70  E-value: 2.23e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081  27 ILNIETStnvC---SVAVSQDGACIFS------KEDHS---G--P-----NHAVKLGIFVDEA-----ISFADshaipLD 82
Cdd:PRK09604   3 ILGIETS---CdetSVAVVDDGRGLLSnvvasqIDLHArygGvvPelasrAHVENIVPLIEEAlkeagLTLED-----ID 74

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 524105081  83 AVAVSCGPGSYTGLRIGVSMAKGICYGRNIKLIAVPTLE--LLTvpVLLTHEPEP--GALL 139
Cdd:PRK09604  75 AIAVTAGPGLVGALLVGVSFAKALALALNKPLIGVNHLEghLLA--PFLEEEPEFpfLALL 133
 
Name Accession Description Interval E-value
TsaB COG1214
tRNA A37 threonylcarbamoyladenosine modification protein TsaB [Translation, ribosomal ...
 27-248 1.34e-75

tRNA A37 threonylcarbamoyladenosine modification protein TsaB [Translation, ribosomal structure and biogenesis]; tRNA A37 threonylcarbamoyladenosine modification protein TsaB is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440827  Cd Length: 227  Bit Score: 228.58  E-value: 1.34e-75
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081  27 ILNIETSTNVCSVAVSQDGACIFSKEDHSGPNHAVKLGIFVDEA-----ISFADshaipLDAVAVSCGPGSYTGLRIGVS 101
Cdd:COG1214    3 ILAIDTSTEACSVALLDDGEVLAEREENDGRGHSERLLPMIDELlaeagLTLSD-----LDAIAVGIGPGSFTGLRIGVA 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081 102 MAKGICYGRNIKLIAVPTLELLTVPVLlthePEPGALLCPMLDARRMEVYSAIYDRSLKEVRGVQAD-VVDADTYKEFLD 180
Cdd:COG1214   78 TAKGLALALGIPLVGVSSLEALAAQAP----RAGAGLVLVAIDARRGEVYWAVYDADDGELERLGEPrVLAPEELAEPLA 153

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 524105081 181 KGKVYFFGNGACKCIEAINHPNAVLLKGI-EPLAKNMFPLAEKRMALENFEDVAYFVPFYLKDFVAKTP 248
Cdd:COG1214  154 GGPVLFVGDGAEAYAELLAEALAVLADALaLPSAAALARLAAARLAAGEAEDPADLEPLYLRDPDAEPP 222
ASKHA_NBD_TsaB cd24032
nucleotide-binding domain (NBD) of tRNA threonylcarbamoyladenosine biosynthesis protein TsaB ...
 27-233 8.67e-54

nucleotide-binding domain (NBD) of tRNA threonylcarbamoyladenosine biosynthesis protein TsaB (previously known as YeaZ) and similar proteins; TsaB, also called t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaB, is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. It may be involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaE. TsaB seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. In fact, it can act as a protease that specifically degrades TsaD in vitro; therefore, TsaB may post-translationally regulate cellular pools of TsaD via proteolytic degradation. TsaB does not show sialoglycoprotease activity against glycophorin A.


Pssm-ID: 466882 [Multi-domain]  Cd Length: 205  Bit Score: 172.46  E-value: 8.67e-54
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081  27 ILNIETSTNVCSVAVSQDGACIFSKEDHSGPNHAVKLGIFVDEA-----ISFADshaipLDAVAVSCGPGSYTGLRIGVS 101
Cdd:cd24032    1 ILAIDTSTSACSVALLKGGKILAEYELDLGRRHSERLLPMIDELlkeagLSLKD-----LDAIAVGIGPGSFTGLRIGLA 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081 102 MAKGICYGRNIKLIAVPTLELLTvpvllTHEPEPGALLCPMLDARRMEVYSAIYDRSLKEVRGVQADVV--DADTYKEFL 179
Cdd:cd24032   76 TAKGLALALGIPLVGVSTLEALA-----QNAPRADGRVLPAIDARRGEVYWALYERDKGGLILEEEEAVlpPEELEEILL 150

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....
gi 524105081 180 DKGKVYFFGNGACKCIEAINHPNAVLLKGIEPLAKNMFPLAEKRMALENFEDVA 233
Cdd:cd24032  151 LKKPAILVGDGWDKYPDLIKENPVLPIELLLPSAADLAPLALAKLAKGETDDEE 204
T6A_YeaZ TIGR03725
tRNA threonylcarbamoyl adenosine modification protein YeaZ; This family describes a protein ...
 27-241 1.18e-52

tRNA threonylcarbamoyl adenosine modification protein YeaZ; This family describes a protein family, YeaZ, now associated with the threonylcarbamoyl adenosine (t6A) tRNA modification. Members of this family may occur as fusions with ygjD (previously gcp) or the ribosomal protein N-acetyltransferase rimI, and is frequently encoded next to rimI. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 274750 [Multi-domain]  Cd Length: 204  Bit Score: 169.37  E-value: 1.18e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081   27 ILNIETSTNVCSVAVSQDGACIFSKEDHSGPNHAVKLGIFVDEA-----ISFADshaipLDAVAVSCGPGSYTGLRIGVS 101
Cdd:TIGR03725   1 ILAIDTSTEALSVALLDDGKVLAERTEPAGRNHSERLLPMIEELlaeagLSLQD-----LDAIAVGVGPGSFTGLRIGLA 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081  102 MAKGICYGRNIKLIAVPTLELLTVPVLLTHEPEPgalLCPMLDARRMEVYSAIYDrsLKEVRGVQadVVDADTYKEFLDK 181
Cdd:TIGR03725  76 TAKGLALALGIPLVGVSSLEALAAQAAAQDGGGP---VLVAIDARRGEVYWGLYD--LKPLEEPA--VLSPEELLELLKE 148

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081  182 GKVYFFGNGACKCIEAINHPNAVLLkgiePLAKNMFPLAEKRMALENFEDVAYFVPFYLK 241
Cdd:TIGR03725 149 LNVLLVGDGAEAYALALLAAAADAL----PDAAALARLALARLKAGEPLDVEELEPLYLR 204
TsaD pfam00814
tRNA N6-adenosine threonylcarbamoyltransferase; This domain can be found in Kae1/Qri7/YgjD, ...
 58-153 1.35e-25

tRNA N6-adenosine threonylcarbamoyltransferase; This domain can be found in Kae1/Qri7/YgjD, products of COG0533 that belong to a small group of 60 proteins that are present in all three domains of life. COG0533 proteins are suggest to play a role in a post translational modification of certain tRNAs. For example, YgjD along with YeaZ, YjeE, and YrdC have been deemed necessary and sufficient for the tRNA modification. This modification involves the formation of N6-threonyl carbamoyl adenosine (t6A) at position 37 in the anti-codon stem loop which is critical for translational speed and accuracy. Structural analysis indicate that YeaZ lacks resemblance to any known protease active site. Together with the absence of a putative zinc-binding motif. Thus the likelyhood of it being a protease, as previously thought, has been negated. EC:2.3.1.234


Pssm-ID: 395656  Cd Length: 272  Bit Score: 101.31  E-value: 1.35e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081   58 NHAVKLGIFVDEAISFADSHAIPLDAVAVSCGPGSYTGLRIGVSMAKGICYGRNIKLIAVPTLELLTVPVLLTHEPEPga 137
Cdd:pfam00814  28 HHSERLMPLIDEALAEAGLSLEDLDAIAVTKGPGLFTGLRVGASFAKGLALALNKPLVGVNHLEAHALAARLETGLEF-- 105

                          90
                  ....*....|....*.
gi 524105081  138 LLCPMLDARRMEVYSA 153
Cdd:pfam00814 106 PVVLLVSGGHTQVYAA 121
ASKHA_NBD_TsaD_bac cd24133
nucleotide-binding domain (NBD) of bacterial tRNA N6-adenosine threonylcarbamoyltransferase ...
 27-135 5.23e-11

nucleotide-binding domain (NBD) of bacterial tRNA N6-adenosine threonylcarbamoyltransferase TsaD and similar proteins; TsaD (EC 2.3.1.234), also called N6-L-threonylcarbamoyladenine synthase, t(6)A synthase, t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD, or tRNA threonylcarbamoyladenosine biosynthesis protein TsaD, is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. It is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.


Pssm-ID: 466983  Cd Length: 328  Bit Score: 61.73  E-value: 5.23e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081  27 ILNIETSTNVCSVAVSQDGACIFS------KEDHS---G--P-----NHAVKLGIFVDEAISFADSHAIPLDAVAVSCGP 90
Cdd:cd24133    1 ILGIETSCDETAVAVVDDGGKILSnvvssqIDLHAkygGvvPeiasrAHLENIIPVVEEALEEAGLTLDDIDAIAVTYGP 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 524105081  91 GSYTGLRIGVSMAKGICYGRNIKLIAVPTLE--LLTvPVLLTHEPEP 135
Cdd:cd24133   81 GLIGALLVGVSFAKALAFALNKPLIGVNHLEghILA-PFLEDPPPEF 126
ASKHA_NBD_Kae1_TsaB-like cd24001
nucleotide-binding domain (NBD) of the Kae1/TsaB-like domain family; The family includes tRNA ...
 27-139 1.85e-10

nucleotide-binding domain (NBD) of the Kae1/TsaB-like domain family; The family includes tRNA N6-adenosine threonylcarbamoyltransferase Kae1/TsaD, tRNA threonylcarbamoyladenosine biosynthesis protein TsaB (previously known as YeaZ), as well as proteins from the NodU/CmcH subfamily. tRNA N6-adenosine threonylcarbamoyltransferase (EC 2.3.1.234) is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. It is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. TsaB is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. It may be involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaE. TsaB seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. The NodU/CmcH family includes NodU from Rhizobium, CmcH from Amycolatopsis lactamdurans, the bifunctional carbamoyltransferase TobZ from Streptoalloteichus tenebrarius, NovN from Streptomyces niveus and NolNO from Sinorhizobium fredii. NodU is a Rhizobium nodulation protein involved in the synthesis of nodulation factors has 6-O-carbamoyltransferase-like activity. 3'-hydroxymethylcephem-O-carbamoyltransferase CmcH (EC 2.1.3.7) is involved in cephamycin (antibiotic) biosynthesis and has 3-hydroxymethylcephem carbamoyltransferase activity. nebramycin 5' synthase TobZ (EC 6.1.2.2) functions as an ATP carbamoyltransferase and tobramycin carbamoyltransferase. Novobiocin biosynthesis protein NovN (EC 2.1.3.12) acts as a carbamoyltransferase that mediates 3'-carbamoylation of the noviosyl ring to produce novobiocin, the final step in the novobiocin biosynthesis pathway. nodulation protein NolNO (EC 2.1.3.-) is involved in the O-carbamoylation of nod factors. The NodU/CmcH subfamily proteins consist of two domains. Only the N-terminal domain shows similarity with Kae1/TsaB-like domain, which belongs to the ASKHA (Acetate and Sugar Kinases/Hsc70/Actin) superfamily of phosphotransferases, all members of which share a common characteristic five-stranded beta sheet occurring in both the N- and C-terminal domains.


Pssm-ID: 466851 [Multi-domain]  Cd Length: 186  Bit Score: 58.23  E-value: 1.85e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081  27 ILNIETSTNVCSVAVSQDGACIFSKED-----HSGPN-------HAVKLGIFVDEAISFADSHAIPLDAVAVSCGPGSYT 94
Cdd:cd24001    1 VLGIEGSAEDTGVAIVDDGGVLANHFEtyvteKTGGYppeaarhHARRIVPLIQEALAESGLTLDDIDAIAFGRGPGLGG 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*.
gi 524105081  95 GLRIGVSMAKGICYGRNIKLIAVPTLE-LLTVPVLLTHEPEPGALL 139
Cdd:cd24001   81 ALRVGATVARGLALAWDKPLIGVNHCIaHAEIAKLKTGATRPVALI 126
PRK09604 PRK09604
tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD;
27-139 2.23e-10

tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD;


Pssm-ID: 236585  Cd Length: 332  Bit Score: 59.70  E-value: 2.23e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081  27 ILNIETStnvC---SVAVSQDGACIFS------KEDHS---G--P-----NHAVKLGIFVDEA-----ISFADshaipLD 82
Cdd:PRK09604   3 ILGIETS---CdetSVAVVDDGRGLLSnvvasqIDLHArygGvvPelasrAHVENIVPLIEEAlkeagLTLED-----ID 74

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 524105081  83 AVAVSCGPGSYTGLRIGVSMAKGICYGRNIKLIAVPTLE--LLTvpVLLTHEPEP--GALL 139
Cdd:PRK09604  75 AIAVTAGPGLVGALLVGVSFAKALALALNKPLIGVNHLEghLLA--PFLEEEPEFpfLALL 133
TsaD COG0533
tRNA A37 threonylcarbamoyltransferase TsaD [Translation, ribosomal structure and biogenesis]; ...
 27-133 3.06e-09

tRNA A37 threonylcarbamoyltransferase TsaD [Translation, ribosomal structure and biogenesis]; tRNA A37 threonylcarbamoyltransferase TsaD is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440299  Cd Length: 333  Bit Score: 56.17  E-value: 3.06e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081  27 ILNIETStnvC---SVAVSQDGACIFS------KEDHSG-----P-----NHAVKLGIFVDEA-----ISFADshaipLD 82
Cdd:COG0533    3 ILGIETS---CdetAAAVVDDGRGLLSnvvasqIDLHARyggvvPelasrAHLENILPLVEEAleeagVTLKD-----ID 74

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 524105081  83 AVAVSCGPGSYTGLRIGVSMAKGICYGRNIKLIAVPTLE--LLTvpVLLTHEP 133
Cdd:COG0533   75 AIAVTAGPGLIGALLVGVSFAKALALALGKPLIGVNHLEghLLA--PFLEDPP 125
ASKHA_NBD_OSGEPL1_QRI7_euk cd24134
nucleotide-binding domain (NBD) of O-sialoglycoprotein endopeptidase-like protein 1 (OSGEPL1) ...
 27-133 9.80e-09

nucleotide-binding domain (NBD) of O-sialoglycoprotein endopeptidase-like protein 1 (OSGEPL1) and similar proteins from eukayotes; The family includes mammalian OSGEPL1 and yeast QRI7, which are the mitochondrial orthologs of the universal Kae1/ TsaD (also known as YgjD) protein. OSGEPL1/QRI7 (EC 2.3.1.234), also called mitochondrial tRNA N6-adenosine threonylcarbamoyltransferase, N6-L-threonylcarbamoyladenine synthase, t(6)A synthase, t(6)A37 threonylcarbamoyladenosine biosynthesis protein, or tRNA threonylcarbamoyladenosine biosynthesis protein, is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in mitochondrial tRNAs that read codons beginning with adenine. It is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. It is involved in mitochondrial genome maintenance.


Pssm-ID: 466984  Cd Length: 330  Bit Score: 54.83  E-value: 9.80e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081  27 ILNIETStnvC---SVA-VSQDGAcIFSKEDHS---------G--PNHAVK-----LGIFVDEAISFADSHAIPLDAVAV 86
Cdd:cd24134    1 VLGIETS---CddtGAAvVDSDGR-ILGEALASqkeiheqygGivPTLAADlhranIPRVVEEALEQAGLSLSDLDAVAV 76

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 524105081  87 SCGPGSYTGLRIGVSMAKGICYGRNIKLIAVPTLE--LLTvpVLLTHEP 133
Cdd:cd24134   77 TVGPGLALCLRVGLEFAKGLAAAHNKPLIPVHHMEahALT--ARLTEEP 123
ASKHA_NBD_TsaD-like cd24097
nucleotide-binding domain (NBD) of TsaD and similar proteins; TsaD (EC 2.3.1.234), also called ...
 27-134 3.37e-05

nucleotide-binding domain (NBD) of TsaD and similar proteins; TsaD (EC 2.3.1.234), also called N6-L-threonylcarbamoyladenine synthase, t(6)A synthase, t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD, or tRNA threonylcarbamoyladenosine biosynthesis protein TsaD, is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. It is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction. The family also includes mammalian OSGEP-like protein 1 (OSGEPL1) and yeast protein Qri7, which are the mitochondrial versions of the universal Kae1/TsaD (also known as YgjD) protein and essential for mitochondrial genome maintenance.


Pssm-ID: 466947  Cd Length: 313  Bit Score: 44.20  E-value: 3.37e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081  27 ILNIETSTNVCSVAVSQDGACIFSK---------EDHSG--P-----NHAVKLGIFVDEAISFADSHAIPLDAVAVSCGP 90
Cdd:cd24097    1 VLGIETSCDETGIAIYDDEKGLLANqlysqvklhADYGGvvPelasrDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGP 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*
gi 524105081  91 GSYTGLRIGVSMAKGICYGRNIKLIAVPTLE-LLTVPVLLTHEPE 134
Cdd:cd24097   81 GLVGALLVGATVGRSLAFAWNVPAIPVHHMEgHLLAPMLEDNPPE 125
ASKHA_NBD_Kae1_TsaD cd24031
nucleotide-binding domain (NBD) of the Kae1/TsaD family tRNA N6-adenosine ...
 27-139 5.02e-04

nucleotide-binding domain (NBD) of the Kae1/TsaD family tRNA N6-adenosine threonylcarbamoyltransferase; tRNA N6-adenosine threonylcarbamoyltransferase (EC 2.3.1.234), also called N6-L-threonylcarbamoyladenine synthase, t(6)A synthase, t(6)A37 threonylcarbamoyladenosine biosynthesis protein, or tRNA threonylcarbamoyladenosine biosynthesis protein, is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. It is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The family includes different orthologous of tRNA N6-adenosine threonylcarbamoyltransferase, such as bacterial kinase-associated endopeptidase 1 (Kae1) and TsaD (also known as YgjD) protein, mammalian O-sialoglycoprotein endopeptidase (OSGEP) and yeast protein Kae1, as well as mammalian OSGEP-like protein 1 (OSGEPL1) and yeast protein Qri7, which are the mitochondrial versions of the universal Kae1/TsaD (also known as YgjD) protein and essential for mitochondrial genome maintenance.


Pssm-ID: 466881  Cd Length: 304  Bit Score: 40.54  E-value: 5.02e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 524105081  27 ILNIETSTNVCSVA-VSQDGACIFSKED-----HSG---P-----NHAVKLGIFVDEAISFADSHAIPLDAVAVSCGPGS 92
Cdd:cd24031    1 VLGIEGSADKTGVGiVDDEGKVLANQLDtyvtpKAGgivPeeaarHHARKIVPLIQEALKESGLTAKDIDLIAYTQGPGL 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 524105081  93 YTGLRIGVSMAKGICYGRNIKLIAVPTLEL-LTVPVLLTHEPEPGALL 139
Cdd:cd24031   81 GGALRVGATVARTLAVAWNKPIIGVNHCIGhLEIPKLNTPAFPPVALY 128
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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