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Conserved domains on  [gi|504282765|ref|WP_014469867|]
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GNAT family N-acetyltransferase [Bacillus amyloliquefaciens]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 18102377)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 93-169 2.44e-09

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


:

Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 51.97  E-value: 2.44e-09
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 504282765  93 LWGDEGHGESMYLHRLAIDRRFAGTGLGRTIMAWAETGVSCPGKTKIRLDCVSENKALQSFYSGMGYESKGLASGYH 169
Cdd:COG0456    4 LLGLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGFEEVGERPNYY 80
yhbS super family cl43796
Predicted N-acetyltransferase YhbS [General function prediction only];
18-160 3.22e-07

Predicted N-acetyltransferase YhbS [General function prediction only];


The actual alignment was detected with superfamily member COG3153:

Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 47.39  E-value: 3.22e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504282765  18 PARKDDAKEVMDLLVSAaewlrdkgssqWSGLLQGEDTHNMREAIAQGHVFLFRQKAELAGVVMLLPeasewdrrlWGDE 97
Cdd:COG3153    3 PATPEDAEAIAALLRAA-----------FGPGREAELVDRLREDPAAGLSLVAEDDGEIVGHVALSP---------VDID 62

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 504282765  98 GHGESMYLHRLAIDRRFAGTGLGRTIMAWAETGVSCPGKTKIRLDCvseNKALQSFYSGMGYE 160
Cdd:COG3153   63 GEGPALLLGPLAVDPEYRGQGIGRALMRAALEAARERGARAVVLLG---DPSLLPFYERFGFR 122
 
Name Accession Description Interval E-value
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 93-169 2.44e-09

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 51.97  E-value: 2.44e-09
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 504282765  93 LWGDEGHGESMYLHRLAIDRRFAGTGLGRTIMAWAETGVSCPGKTKIRLDCVSENKALQSFYSGMGYESKGLASGYH 169
Cdd:COG0456    4 LLGLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGFEEVGERPNYY 80
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
18-160 3.22e-07

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 47.39  E-value: 3.22e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504282765  18 PARKDDAKEVMDLLVSAaewlrdkgssqWSGLLQGEDTHNMREAIAQGHVFLFRQKAELAGVVMLLPeasewdrrlWGDE 97
Cdd:COG3153    3 PATPEDAEAIAALLRAA-----------FGPGREAELVDRLREDPAAGLSLVAEDDGEIVGHVALSP---------VDID 62

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 504282765  98 GHGESMYLHRLAIDRRFAGTGLGRTIMAWAETGVSCPGKTKIRLDCvseNKALQSFYSGMGYE 160
Cdd:COG3153   63 GEGPALLLGPLAVDPEYRGQGIGRALMRAALEAARERGARAVVLLG---DPSLLPFYERFGFR 122
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 31-159 1.65e-06

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 44.82  E-value: 1.65e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504282765   31 LVSAAEWLRDKGSSQWSglLQGEDTHNMREAIAQGHVFLFRQKAELAGVVMLLPEASEWDRrlwgdeghgesMYLHRLAI 110
Cdd:pfam00583   1 LEALYELLSEEFPEPWP--DEPLDLLEDWDEDASEGFFVAEEDGELVGFASLSIIDDEPPV-----------GEIEGLAV 67

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 504282765  111 DRRFAGTGLGRTIMAWAETGVSCPGKTKIRLDCVSENKALQSFYSGMGY 159
Cdd:pfam00583  68 APEYRGKGIGTALLQALLEWARERGCERIFLEVAADNLAAIALYEKLGF 116
PRK03624 PRK03624
putative acetyltransferase; Provisional
 67-160 1.93e-06

putative acetyltransferase; Provisional


Pssm-ID: 235142 [Multi-domain]  Cd Length: 140  Bit Score: 45.30  E-value: 1.93e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504282765  67 VFLFRQKAELAGVVMLlpeasewdrrlwGDEGHGESMYlhRLAIDRRFAGTGLGRTIMAWAE---TGVSCPgktKIRLDC 143
Cdd:PRK03624  47 FLVAEVGGEVVGTVMG------------GYDGHRGWAY--YLAVHPDFRGRGIGRALVARLEkklIARGCP---KINLQV 109

                         90
                 ....*....|....*..
gi 504282765 144 VSENKALQSFYSGMGYE 160
Cdd:PRK03624 110 REDNDAVLGFYEALGYE 126
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 67-142 2.84e-03

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 34.94  E-value: 2.84e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 504282765  67 VFLFRQKAELAGVVMLLPeasewdrrlwgDEGHGESMYLHRLAIDRRFAGTGLGRTIMAWAETGVSCPGKTKIRLD 142
Cdd:cd04301    1 FLVAEDDGEIVGFASLSP-----------DGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
 
Name Accession Description Interval E-value
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 93-169 2.44e-09

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 51.97  E-value: 2.44e-09
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 504282765  93 LWGDEGHGESMYLHRLAIDRRFAGTGLGRTIMAWAETGVSCPGKTKIRLDCVSENKALQSFYSGMGYESKGLASGYH 169
Cdd:COG0456    4 LLGLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGFEEVGERPNYY 80
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
18-160 3.22e-07

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 47.39  E-value: 3.22e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504282765  18 PARKDDAKEVMDLLVSAaewlrdkgssqWSGLLQGEDTHNMREAIAQGHVFLFRQKAELAGVVMLLPeasewdrrlWGDE 97
Cdd:COG3153    3 PATPEDAEAIAALLRAA-----------FGPGREAELVDRLREDPAAGLSLVAEDDGEIVGHVALSP---------VDID 62

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 504282765  98 GHGESMYLHRLAIDRRFAGTGLGRTIMAWAETGVSCPGKTKIRLDCvseNKALQSFYSGMGYE 160
Cdd:COG3153   63 GEGPALLLGPLAVDPEYRGQGIGRALMRAALEAARERGARAVVLLG---DPSLLPFYERFGFR 122
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 100-176 1.12e-06

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 45.81  E-value: 1.12e-06
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 504282765 100 GESMYLHRLAIDRRFAGTGLGRTIMAWAETGVSCPGKTKIRLDCVSENKALQSFYSGMGYE--SKGLASGYHLFEKEMS 176
Cdd:COG0454   56 DKVLELKRLYVLPEYRGKGIGKALLEALLEWARERGCTALELDTLDGNPAAIRFYERLGFKeiERYVAYVGGEFEKELS 134
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 31-159 1.65e-06

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 44.82  E-value: 1.65e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504282765   31 LVSAAEWLRDKGSSQWSglLQGEDTHNMREAIAQGHVFLFRQKAELAGVVMLLPEASEWDRrlwgdeghgesMYLHRLAI 110
Cdd:pfam00583   1 LEALYELLSEEFPEPWP--DEPLDLLEDWDEDASEGFFVAEEDGELVGFASLSIIDDEPPV-----------GEIEGLAV 67

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 504282765  111 DRRFAGTGLGRTIMAWAETGVSCPGKTKIRLDCVSENKALQSFYSGMGY 159
Cdd:pfam00583  68 APEYRGKGIGTALLQALLEWARERGCERIFLEVAADNLAAIALYEKLGF 116
PRK03624 PRK03624
putative acetyltransferase; Provisional
 67-160 1.93e-06

putative acetyltransferase; Provisional


Pssm-ID: 235142 [Multi-domain]  Cd Length: 140  Bit Score: 45.30  E-value: 1.93e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504282765  67 VFLFRQKAELAGVVMLlpeasewdrrlwGDEGHGESMYlhRLAIDRRFAGTGLGRTIMAWAE---TGVSCPgktKIRLDC 143
Cdd:PRK03624  47 FLVAEVGGEVVGTVMG------------GYDGHRGWAY--YLAVHPDFRGRGIGRALVARLEkklIARGCP---KINLQV 109

                         90
                 ....*....|....*..
gi 504282765 144 VSENKALQSFYSGMGYE 160
Cdd:PRK03624 110 REDNDAVLGFYEALGYE 126
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
66-163 3.02e-04

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 39.01  E-value: 3.02e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504282765  66 HVFLFRQKaELAGVVMLLPEAsewdrrlwGDEGHGEsmylhRLAIDRRFAGTGLGRTIMAWAETGVSCPGKTKIRLDCvs 145
Cdd:COG2153   36 HLLAYDDG-ELVATARLLPPG--------DGEAKIG-----RVAVLPEYRGQGLGRALMEAAIEEARERGARRIVLSA-- 99

                         90
                 ....*....|....*...
gi 504282765 146 eNKALQSFYSGMGYESKG 163
Cdd:COG2153  100 -QAHAVGFYEKLGFVPVG 116
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 54-160 6.69e-04

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 38.02  E-value: 6.69e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504282765   54 DTHNMREAIAQGHVFLF--RQKAELAGVVMLlpeasewdrrlwGDEGHgesmyLHRLAIDRRFAGTGLGRTIMAWAETGV 131
Cdd:pfam13673  18 SPEALRERIDQGEYFFFvaFEGGQIVGVIAL------------RDRGH-----ISLLFVDPDYQGQGIGKALLEAVEDYA 80

                          90       100
                  ....*....|....*....|....*....
gi 504282765  132 SCPGKTKIRLDcVSENKALQSFYSGMGYE 160
Cdd:pfam13673  81 EKDGIKLSELT-VNASPYAVPFYEKLGFR 108
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 65-160 8.43e-04

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 36.66  E-value: 8.43e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504282765   65 GHVFLFRQKAELAGVVMLLPEasewdrrlwgdeGHGESMYLHRLAIDRRFAGTGLGRTIMAWAETgvsCPGKTKIRLDCV 144
Cdd:pfam13508   3 GRFFVAEDDGKIVGFAALLPL------------DDEGALAELRLAVHPEYRGQGIGRALLEAAEA---AAKEGGIKLLEL 67

                          90
                  ....*....|....*.
gi 504282765  145 SENKALQSFYSGMGYE 160
Cdd:pfam13508  68 ETTNRAAAFYEKLGFE 83
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 67-142 2.84e-03

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 34.94  E-value: 2.84e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 504282765  67 VFLFRQKAELAGVVMLLPeasewdrrlwgDEGHGESMYLHRLAIDRRFAGTGLGRTIMAWAETGVSCPGKTKIRLD 142
Cdd:cd04301    1 FLVAEDDGEIVGFASLSP-----------DGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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