Uncharacterized Fe-S cluster protein [Function unknown];

                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 499428361  5 INNECIACDACAEECPNGAIEEGDPIYSIDPDVCTECvgsydePSCLSVCPVDAIIPDPDNIENMEE 71
Cdd:COG2768   9 DEEKCIGCGACVKVCPVGAISIEDGKAVIDPEKCIGC------GACIEVCPVGAIKIEWEEDEEFQE 69

uncharacterized subfamily of DMSO Reductase beta subunit family; This family consists of the small beta iron-sulfur (FeS) subunit of the DMSO Reductase (DMSOR) family. Members of this family also contain a large, periplasmic molybdenum-containing alpha subunit and may have a small gamma subunit as well. Examples of heterodimeric members with alpha and beta subunits include arsenite oxidase, and tungsten-containing formate dehydrogenase (FDH-T) while heterotrimeric members containing alpha, beta, and gamma subunits include formate dehydrogenase-N (FDH-N), and nitrate reductase (NarGHI). The beta subunit contains four Fe4/S4 and/or Fe3/S4 clusters which transfer the electrons from the alpha subunit to a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups. The reducing equivalents are then transferred to menaquinone, which finally reduces the electron-accepting enzyme system.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 499428361   5 INNECIACDACAEECPNGAIEegdpiySIDPDVCTECVGsYDEPSCLSVCPVDAII 60
Cdd:cd16367   84 ISDACCGCGNCASACPYGAIQ------MVRAVKCDLCAG-YAGPACVSACPTGAAI 132

4Fe-4S dicluster domain; Superfamily includes proteins containing domains which bind to iron-sulfur clusters. Members include bacterial ferredoxins, various dehydrogenases, and various reductases. Structure of the domain is an alpha-antiparallel beta sandwich. Domain contains two 4Fe4S clusters.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 499428361   9 CIACDACAEECPNGAIE-------EGDPIYSIDPDVCTECvgsydePSCLSVCPVDA 58
Cdd:pfam12838  1 CIGCGACVAACPVGAITldevgekKGTKTVVIDPERCVGC------GACVAVCPTGA 51

Uncharacterized Fe-S cluster protein [Function unknown];

                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 499428361  5 INNECIACDACAEECPNGAIEEGDPIYSIDPDVCTECvgsydePSCLSVCPVDAIIPDPDNIENMEE 71
Cdd:COG2768   9 DEEKCIGCGACVKVCPVGAISIEDGKAVIDPEKCIGC------GACIEVCPVGAIKIEWEEDEEFQE 69

Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I) [Energy production and conversion]; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I) is part of the Pathway/BioSystem: NADH dehydrogenase

                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*....
gi 499428361  9 CIACDACAEECPNGAIE----EGDPIYSIDPDVCTECVgsydepSCLSVCPVDAIIPDP 63
Cdd:COG1143   4 CIGCGLCVRVCPVDAITiedgEPGKVYVIDPDKCIGCG------LCVEVCPTGAISMTP 56

MinD superfamily P-loop ATPase, contains an inserted ferredoxin domain [General function prediction only];

                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 499428361  9 CIACDACAEECPNGAIEEGDP-IYSIDPDVCTECVGsydepsCLSVCPVDAIIPDPDNIENM 69
Cdd:COG1149  13 CIGCGLCVEVCPEGAIKLDDGgAPVVDPDLCTGCGA------CVGVCPTGAITLEEREAGKI 68

Heterodisulfide reductase, subunit A (polyferredoxin) [Energy production and conversion];

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|..
gi 499428361   9 CIACDACAEECPNGAIEE-GDPIYSIDPDVCTECvGsydepSCLSVCPVDAI 59
Cdd:COG1148  498 CTGCGRCVEVCPYGAISIdEKGVAEVNPALCKGC-G-----TCAAACPSGAI 543

Iron only hydrogenase large subunit, C-terminal domain [Energy production and conversion];

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 499428361   7 NECIACDACAEECPNGAIEEGDPIYSIDPDVCTECvGsydepSCLSVCPVDAI 59
Cdd:COG4624   91 EKCKNCYPCVRACPVKAIKVDDGKAEIDEEKCISC-G-----QCVAVCPFGAI 137

Pyruvate:ferredoxin oxidoreductase or related 2-oxoacid:ferredoxin oxidoreductase, delta subunit [Energy production and conversion]; Pyruvate:ferredoxin oxidoreductase or related 2-oxoacid:ferredoxin oxidoreductase, delta subunit is part of the Pathway/BioSystem: Pyruvate oxidation

                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|..
gi 499428361  9 CIACDACAEECPNGAIEEGD-PIYSIDPDVCTECvgsydePSCLSVCPVDAI 59
Cdd:COG1144  32 CIGCGLCWIVCPDGAIRVDDgKYYGIDYDYCKGC------GICAEVCPVKAI 77

Fe-S cluster domain-containing protein;

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 499428361   9 CIACDACAEECPNGAIEEGDPIYSIDPDVCTECvgsydePSCLSVCPVDAIIP 61
Cdd:PRK07118 215 CIGCGKCVKACPAGAITMENNLAVIDQEKCTSC------GKCVEKCPTKAIRI 261

EFR1 family ferrodoxin; Members of the family have a C-terminal ferrodoxin domain, with eight conserved Cys residues in two CxxCxxCxxxCP motifs, each of which binds a 4Fe-4S cluster. The N-terminal region resembles flavodoxin domains, with some members of the family recognized by Pfam models PF12724 (Flavodoxin_5) or PF00258 (Flavodoxin_1).

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 499428361   5 INNECIACDACAEECPNGAI--EEGDPIYSidpDVCTECVGsydepsCLSVCPVDAI 59
Cdd:NF038196 183 VTDKCIGCGICAKVCPVNNIemEDGKPVWG---HNCTHCLA------CIHRCPKEAI 230

NapF, iron-sulfur subunit of periplasmic nitrate reductase; This family contains NapF protein, the iron-sulfur subunit of periplasmic nitrate reductase. The periplasmic nitrate reductase NapABC of Escherichia coli likely functions during anaerobic growth in low-nitrate environments; napF operon expression is activated by cyclic AMP receptor protein (Crp). NapF is a subfamily of the beta subunit of DMSO reductase (DMSOR) family. DMSOR family members have a large, periplasmic molybdenum-containing alpha subunit as well as a small beta FeS subunit, and may also have a small gamma subunit. The beta subunit contains four Fe4/S4 and/or Fe3/S4 clusters which transfer the electrons from the alpha subunit to a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups. The reducing equivalents are then transferred to menaquinone, which finally reduces the electron-accepting enzyme system.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 499428361  10 IACDACAEECPNGAIE-----EGDPIYSIDPDVCTECVGsydepsCLSVCPVDAI 59
Cdd:cd10564   86 VECRSCQDACPTQAIRfrprlGGIALPELDADACTGCGA------CVSVCPVGAI 134

Uncharacterized polyferredoxin-like protein; This family contains uncharacterized polyferredoxin protein similar to Methanobacterium thermoautotrophicum MvhB. The mvhB is a gene of the methylviologen-reducing hydrogenase operon. It is predicted to contain 12 [4Fe-4S] clusters, and was therefore suggested to be a polyferredoxin. As a subfamily of the beta subunit of the DMSO Reductase (DMSOR) family, it is predicted to function as electron carrier in the reducing reaction.

                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499428361   9 CIACDACAEECPNGAIEEGDPI-----YSIDPDVCTECvgsydePSCLSVCPVDAIIPDPDNIENMEELK 73
Cdd:cd10549    8 CIGCGICVKACPTDAIELGPNGaiargPEIDEDKCVFC------GACVEVCPTGAIELTPEGKEYVPKEK 71

4Fe-4S dicluster domain; Superfamily includes proteins containing domains which bind to iron-sulfur clusters. Members include bacterial ferredoxins, various dehydrogenases, and various reductases. Structure of the domain is an alpha-antiparallel beta sandwich. Domain contains two 4Fe4S clusters.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 499428361   9 CIACDACAEECPNGAIE-------EGDPIYSIDPDVCTECvgsydePSCLSVCPVDA 58
Cdd:pfam12838  1 CIGCGACVAACPVGAITldevgekKGTKTVVIDPERCVGC------GACVAVCPTGA 51

NADH-quinone oxidoreductase, chain I; This model represents the I subunit (one of 14: A->N) of the NADH-quinone oxidoreductase complex I which generally couples NADH and ubiquinone oxidation/reduction in bacteria and mammalian mitochondria, but may act on NADPH and/or plastoquinone in cyanobacteria and plant chloroplasts. This model excludes "I" subunits from the closely related F420H2 dehydrogenase and formate hydrogenlyase complexes. [Energy metabolism, Electron transport]

                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 499428361    9 CIACDACAEECPNGAI----EEGDP------IYSIDPDVCTECvgsydePSCLSVCPVDAIIPDPD 64
Cdd:TIGR01971  45 CIGCTLCAAVCPADAIrvvpAEGEDgkrrlkFYEINFGRCIFC------GLCEEACPTDAIVLTPE 104

Polyferredoxin NapH [Energy production and conversion];

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....
gi 499428361   7 NECIACDACAEECPNG-AIEEGDPIYSidpdvctECVGSYDepsCLSVCPVDAI 59
Cdd:COG0348  210 GDCIDCGLCVKVCPMGiDIRKGEINQS-------ECINCGR---CIDACPKDAI 253

electron transport complex subunit RsxB;

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 499428361   9 CIACDACAEECPNGAIeEGDP--IYSIDPDVCTECvgsydePSCLSVCPVDAI 59
Cdd:PRK06991  87 CIGCTLCMQACPVDAI-VGAPkqMHTVLADLCTGC------DLCVPPCPVDCI 132

HycB, HydN and similar proteins; This family includes HycB, the FeS subunit of a membrane-associated formate hydrogenlyase system (FHL-1) in Escherichia coli that breaks down formate, produced during anaerobic fermentation, to H2 and CO2. FHL-1 consists of formate dehydrogenase H (FDH-H) and the hydrogenase 3 complex (Hyd-3). HycB is thought to code for the [4Fe-4S] ferredoxin subunit of hydrogenase 3, which functions as an intermediate electron carrier protein between hydrogenase 3 and formate dehydrogenase. HydN codes for the [4Fe-4S] ferredoxin subunit of FDH-H; a hydN in-frame deletion mutation causes only weak reduction in hydrogenase activity, but loss of more than 60% of FDH-H activity. This pathway is only active at low pH and high formate concentrations, and is thought to provide a detoxification/de-acidification system countering the buildup of formate during fermentation.

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 499428361   9 CIACDACAEECPNGAIE---EGDPIYSIDPDV------CTECVGSYDEPSCLSVCPVDAI 59
Cdd:cd10554   87 CIGCKLCVLACPFGAIEmapTTVPGVDWERGPravavkCDLCAGREGGPACVEACPTKAL 146

HycB, HydN and similar proteins; This family includes HycB, the FeS subunit of a membrane-associated formate hydrogenlyase system (FHL-1) in Escherichia coli that breaks down formate, produced during anaerobic fermentation, to H2 and CO2. FHL-1 consists of formate dehydrogenase H (FDH-H) and the hydrogenase 3 complex (Hyd-3). HycB is thought to code for the [4Fe-4S] ferredoxin subunit of hydrogenase 3, which functions as an intermediate electron carrier protein between hydrogenase 3 and formate dehydrogenase. HydN codes for the [4Fe-4S] ferredoxin subunit of FDH-H; a hydN in-frame deletion mutation causes only weak reduction in hydrogenase activity, but loss of more than 60% of FDH-H activity. This pathway is only active at low pH and high formate concentrations, and is thought to provide a detoxification/de-acidification system countering the buildup of formate during fermentation.

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*....
gi 499428361  15 CAEECPNGAIEEGDPIYSIDPDVCTECvgsydePSCLSVCPVDAIIPDP 63
Cdd:cd10554   64 CANVCPVGAISQEDGVVQVDEERCIGC------KLCVLACPFGAIEMAP 106

putative oxidoreductase Fe-S binding subunit; Reviewed

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 499428361   2 SLMINNE-CIACDACAEECPNGAIE-----EGDPIYSIDPDVCTECVGSYDEPSCLSVCPVDAI 59
Cdd:PRK12769  79 SIQVNQQkCIGCKSCVVACPFGTMQivltpVAAGKVKATAHKCDLCAGRENGPACVENCPADAL 142

Rnf electron transport complex subunit RnfB;

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 499428361   1 MSLMINNECIACDACAEECPNGAIEEGDP-IYSIDPDVCTECvgsydePSCLSVCPVDAI 59
Cdd:PRK08764  79 VAWIVEADCIGCTKCIQACPVDAIVGGAKhMHTVIAPLCTGC------ELCVPACPVDCI 132

uncharacterized subfamily of beta subunit of formate dehydrogenase; This subfamily includes the beta-subunit of formate dehydrogenases that are as yet uncharacterized. Members of the DMSO reductase family include formate dehydrogenase N and O (FDH-N, FDH-O) and tungsten-containing formate dehydrogenase (W-FDH) and other similar proteins. FDH-N, a major component of nitrate respiration of Escherichia coli, is involved in the major anaerobic respiratory pathway in the presence of nitrate, catalyzing the oxidation of formate to carbon dioxide at the expense of nitrate reduction to nitrite. It forms a heterotrimer; the alpha-subunit (FDH-G) is the catalytic site of formate oxidation and membrane-associated, incorporating a selenocysteine (SeCys) residue and a [4Fe/4S] cluster in addition to two bis-MGD cofactors, the beta subunit (FDH-H) contains four [4Fe/4S] clusters which transfer the electrons from the alpha subunit to the gamma-subunit (FDH-I), a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups. W-FDH contains a tungsten instead of molybdenum at the catalytic center. This enzyme seems to be exclusively found in organisms such as hyperthermophilic archaea that live in extreme environments. It is a heterodimer of a large and a small subunit; the large subunit harbors the W site and one [4Fe-4S] center and the small subunit, containing three [4Fe-4S] clusters, functions to transfer electrons.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 499428361   6 NNECIACDACAEECPNGAIEEGDPIYSIDPdvCTECVGSYDE---PSCLSVCPVDAI 59
Cdd:cd10562   99 EDKCIGCGYCVAACPFDVPRYDETTNKITK--CTLCFDRIENgmqPACVKTCPTGAL 153

electron transport complex protein RnfB; Provisional

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 499428361   7 NECIACDACAEECPNGAI-EEGDPIYSIDPDVCTECvgsydePSCLSVCPVDAI--IPDPDNIEN 68
Cdd:PRK05113 114 DNCIGCTKCIQACPVDAIvGATKAMHTVISDLCTGC------DLCVAPCPTDCIemIPVAETPDN 172

electron transport complex, RnfABCDGE type, B subunit; The six subunit complex RnfABCDGE in Rhodobacter capsulatus encodes an apparent NADH oxidoreductase responsible for electron transport to nitrogenase, necessary for nitrogen fixation. A closely related complex in E. coli, RsxABCDGE (Reducer of SoxR), reduces the 2Fe-2S-containing superoxide sensor SoxR, active as a transcription factor when oxidized. This family of putative NADH oxidoreductase complexes exists in many of the same species as the related NQR, a Na(+)-translocating NADH-quinone reductase, but is distinct. This model describes the B subunit. [Energy metabolism, Electron transport]

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 499428361    7 NECIACDACAEECPNGAIEEG-DPIYSIDPDVCTECvgsydePSCLSVCPVDAI 59
Cdd:TIGR01944 113 DNCIGCTKCIQACPVDAIVGAaKAMHTVIADECTGC------DLCVEPCPTDCI 160

Na+-translocating ferredoxin:NAD+ oxidoreductase RNF, RnfB subunit [Energy production and conversion]; Na+-translocating ferredoxin:NAD+ oxidoreductase RNF, RnfB subunit is part of the Pathway/BioSystem: Na+-translocating Fd:NADH oxidoreductase

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 499428361   6 NNECIACDACAEECPNGAIeEGDP--IYSIDPDVCTECvgsydePSCLSVCPVDAI 59
Cdd:COG2878  136 EYGCIGCGDCIKACPFDAI-VGAAkgMHTVDEDKCTGC------GLCVEACPVDCI 184

uncharacterized subfamily of DMSO Reductase beta subunit family; This family consists of the small beta iron-sulfur (FeS) subunit of the DMSO Reductase (DMSOR) family. Members of this family also contain a large, periplasmic molybdenum-containing alpha subunit and may have a small gamma subunit as well. Examples of heterodimeric members with alpha and beta subunits include arsenite oxidase, and tungsten-containing formate dehydrogenase (FDH-T) while heterotrimeric members containing alpha, beta, and gamma subunits include formate dehydrogenase-N (FDH-N), and nitrate reductase (NarGHI). The beta subunit contains four Fe4/S4 and/or Fe3/S4 clusters which transfer the electrons from the alpha subunit to a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups. The reducing equivalents are then transferred to menaquinone, which finally reduces the electron-accepting enzyme system.

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 499428361   9 CIACDACAEECPNGAIEEGDPIYSID-----------PDVCTECvgsyDEPSCLSVCPVDAIIPDPDNI 66
Cdd:cd16369   11 CIGCRACVAACRECGTHRGKSMIHVDyidrgestqtaPTVCMHC----EDPTCAEVCPADAIKVTEDGV 75

electron transport protein HydN;

                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 499428361   9 CIACDACAEECPNGAIE-----------EGDPI--YSIDPDVCTECVGSYDEPSCLSVCPVDAIIP-DPDNIENM 69
Cdd:PRK10330  89 CIGCKTCVVACPYGAMEvvvrpvirnsgAGLNVraEKAEANKCDLCNHREDGPACMAACPTHALICvDRNKLEQL 163