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Conserved domains on  [gi|490391141|gb|AGL12180|]
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putative thioesterase [Actinoplanes sp. N902-109]

Protein Classification

thioesterase II family protein( domain architecture ID 10007057)

thioesterase II family protein such as gramicidin S biosynthesis protein GrsT, S-acyl fatty acid synthase thioesterase, and the surfactin synthase thioesterase subunit, which is involved in the surfactin biosynthesis pathway

CATH:  3.40.50.1820
EC:  3.1.2.-
Gene Ontology:  GO:0009058
PubMed:  3732600

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GrsT COG3208
Surfactin synthase thioesterase subunit [Secondary metabolites biosynthesis, transport and ...
 18-251 9.33e-109

Surfactin synthase thioesterase subunit [Secondary metabolites biosynthesis, transport and catabolism];


:

Pssm-ID: 442441 [Multi-domain]  Cd Length: 237  Bit Score: 313.33  E-value: 9.33e-109
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141  18 PAPQSSVRLVCFPYAGGSGSFFLPISRALAPTVEVLAVQYPARQTRRLEPGIDNIPDFADQIFAAVRQSTDKPLAFFGHS 97
Cdd:COG3208    1 PRPDARLRLFCFPYAGGSASAYRPWAAALPPDIEVLAVQLPGRGDRLGEPPLTSLEELADDLAEELAPLLDRPFALFGHS 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141  98 MGAVLAYEVALRFQQAGLPSPVRLFVSGRRAPSKYRDERF-HAAPDAEFIAELKGLGGPNSAFLNDPEMLEMVLPALRSD 176
Cdd:COG3208   81 MGALLAFELARRLERRGRPLPAHLFVSGRRAPHLPRRRRPlHDLSDAELLAELRRLGGTPEEVLADPELLELFLPILRAD 160

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 490391141 177 YTAIERYRHEPGRTLDCPISAFVGDSDPRASLDEVRAWAQHTTGPFDLEIFQGDHFYLIGQGPQLIELIAKKLAG 251
Cdd:COG3208  161 FRLLETYRYTPGPPLDCPITALGGDDDPLVSPEELAAWREHTTGPFRLRVFPGGHFFLRDHPAELLALIRAALAA 235
 
Name Accession Description Interval E-value
GrsT COG3208
Surfactin synthase thioesterase subunit [Secondary metabolites biosynthesis, transport and ...
 18-251 9.33e-109

Surfactin synthase thioesterase subunit [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 442441 [Multi-domain]  Cd Length: 237  Bit Score: 313.33  E-value: 9.33e-109
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141  18 PAPQSSVRLVCFPYAGGSGSFFLPISRALAPTVEVLAVQYPARQTRRLEPGIDNIPDFADQIFAAVRQSTDKPLAFFGHS 97
Cdd:COG3208    1 PRPDARLRLFCFPYAGGSASAYRPWAAALPPDIEVLAVQLPGRGDRLGEPPLTSLEELADDLAEELAPLLDRPFALFGHS 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141  98 MGAVLAYEVALRFQQAGLPSPVRLFVSGRRAPSKYRDERF-HAAPDAEFIAELKGLGGPNSAFLNDPEMLEMVLPALRSD 176
Cdd:COG3208   81 MGALLAFELARRLERRGRPLPAHLFVSGRRAPHLPRRRRPlHDLSDAELLAELRRLGGTPEEVLADPELLELFLPILRAD 160

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 490391141 177 YTAIERYRHEPGRTLDCPISAFVGDSDPRASLDEVRAWAQHTTGPFDLEIFQGDHFYLIGQGPQLIELIAKKLAG 251
Cdd:COG3208  161 FRLLETYRYTPGPPLDCPITALGGDDDPLVSPEELAAWREHTTGPFRLRVFPGGHFFLRDHPAELLALIRAALAA 235
Thioesterase pfam00975
Thioesterase domain; Peptide synthetases are involved in the non-ribosomal synthesis of ...
 25-249 1.15e-62

Thioesterase domain; Peptide synthetases are involved in the non-ribosomal synthesis of peptide antibiotics. Next to the operons encoding these enzymes, in almost all cases, are genes that encode proteins that have similarity to the type II fatty acid thioesterases of vertebrates. There are also modules within the peptide synthetases that also share this similarity. With respect to antibiotic production, thioesterases are required for the addition of the last amino acid to the peptide antibiotic, thereby forming a cyclic antibiotic. Thioesterases (non-integrated) have molecular masses of 25-29 kDa.


Pssm-ID: 395776 [Multi-domain]  Cd Length: 223  Bit Score: 195.68  E-value: 1.15e-62
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141   25 RLVCFPYAGGSGSFFLPISRALAPTVEVLAVQYPARQtrRLEPGIDNIPDFADQIFAAVRQS-TDKPLAFFGHSMGAVLA 103
Cdd:pfam00975   2 PLFCFPPAGGSASSFRSLARRLPPPAEVLAVQYPGRG--RGEPPLNSIEALADEYAEALRQIqPEGPYALFGHSMGGMLA 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141  104 YEVALRFQQAGLPsPVRLFVSGRRAPSKYRDERFHAAPDAEFIAELKGLGGPNSAFLNDPEMLEMVLPALRSDYTAIERY 183
Cdd:pfam00975  80 FEVARRLERQGEA-VRSLFLSDASAPHTVRYEASRAPDDDEVVAEFTDEGGTPEELLEDEELLSMLLPALRADYRALESY 158

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 490391141  184 RHEPGRTLDcpISAFVGDSDPRASLDEVRAWA-QHTTGPFDLEIFQGDHFYLIGQGPQLIELIAKKL 249
Cdd:pfam00975 159 SCPPLDAQS--ATLFYGSDDPLHDADDLAEWVrDHTPGEFDVHVFDGDHFYLIEHLEAVLEIIEAKL 223
PKS_TE smart00824
Thioesterase; Peptide synthetases are involved in the non-ribosomal synthesis of peptide ...
 81-235 4.42e-04

Thioesterase; Peptide synthetases are involved in the non-ribosomal synthesis of peptide antibiotics. Next to the operons encoding these enzymes, in almost all cases, are genes that encode proteins that have similarity to the type II fatty acid thioesterases of vertebrates. There are also modules within the peptide synthetases that also share this similarity. With respect to antibiotic production, thioesterases are required for the addition of the last amino acid to the peptide antibiotic, thereby forming a cyclic antibiotic. Thioesterases (non-integrated) have molecular masses of 25-29 kDa.


Pssm-ID: 214835 [Multi-domain]  Cd Length: 212  Bit Score: 40.29  E-value: 4.42e-04
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141    81 AAVRQSTDKPLAFFGHSMGAVLAYEVALRFQQAGL-PSPVRLFVSgrRAPSKYRDERFHAAPDAEFIAELKGLGGPNSAF 159
Cdd:smart00824  56 AVLRAAGGRPFVLVGHSSGGLLAHAVAARLEARGIpPAAVVLLDT--YPPGDPAPEGWLPELLRGVFEREDSFVPMDDAR 133

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141   160 LndpemlemvlpalrsdyTAIERYRHEPGRTLDCPISA---FVGDSDP--RASLDEVRAWAQHTTGPFDLEIFQGDHFYL 234
Cdd:smart00824 134 L-----------------TAMGAYLRLFGGWTPGPVAAptlLVRASEPlaEWPDEDPDGWRAHWPLPHTVVDVPGDHFTM 196


                   .
gi 490391141   235 I 235
Cdd:smart00824 197 M 197
entF PRK10252
enterobactin non-ribosomal peptide synthetase EntF;
26-250 1.09e-03

enterobactin non-ribosomal peptide synthetase EntF;


Pssm-ID: 236668 [Multi-domain]  Cd Length: 1296  Bit Score: 40.03  E-value: 1.09e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141   26 LVCFPYAGGSGSFFLPISRALAPTVEVLAVQYPARQTRRLEPgiDNIPDFADQIFAAVRQSTDK-PLAFFGHSMGAVLAY 104
Cdd:PRK10252 1071 LFCFHPASGFAWQFSVLSRYLDPQWSIYGIQSPRPDGPMQTA--TSLDEVCEAHLATLLEQQPHgPYHLLGYSLGGTLAQ 1148

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141  105 EVALRFQQAG-----------LPSPVRLFvsgRRAPSKYRDERFHAAPDAE---FIAELKGLGGpnsaflndpemlEMVL 170
Cdd:PRK10252 1149 GIAARLRARGeevaflglldtWPPETQNW---REKEANGLDPEVLAEIDREreaFLAAQQGSLS------------TELF 1213

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141  171 PALRSDYT-AIERYRHEPGRTLDCPISAFVGDSDPRASLDEVRAWAQHTTGpfdLEIFQGD--HFYLIgqGPQLIELIAK 247
Cdd:PRK10252 1214 TTIEGNYAdAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPEQAWSPWIAE---LDVYRQDcaHVDII--SPEAFEKIGP 1288


                  ...
gi 490391141  248 KLA 250
Cdd:PRK10252 1289 ILR 1291
 
Name Accession Description Interval E-value
GrsT COG3208
Surfactin synthase thioesterase subunit [Secondary metabolites biosynthesis, transport and ...
 18-251 9.33e-109

Surfactin synthase thioesterase subunit [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 442441 [Multi-domain]  Cd Length: 237  Bit Score: 313.33  E-value: 9.33e-109
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141  18 PAPQSSVRLVCFPYAGGSGSFFLPISRALAPTVEVLAVQYPARQTRRLEPGIDNIPDFADQIFAAVRQSTDKPLAFFGHS 97
Cdd:COG3208    1 PRPDARLRLFCFPYAGGSASAYRPWAAALPPDIEVLAVQLPGRGDRLGEPPLTSLEELADDLAEELAPLLDRPFALFGHS 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141  98 MGAVLAYEVALRFQQAGLPSPVRLFVSGRRAPSKYRDERF-HAAPDAEFIAELKGLGGPNSAFLNDPEMLEMVLPALRSD 176
Cdd:COG3208   81 MGALLAFELARRLERRGRPLPAHLFVSGRRAPHLPRRRRPlHDLSDAELLAELRRLGGTPEEVLADPELLELFLPILRAD 160

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 490391141 177 YTAIERYRHEPGRTLDCPISAFVGDSDPRASLDEVRAWAQHTTGPFDLEIFQGDHFYLIGQGPQLIELIAKKLAG 251
Cdd:COG3208  161 FRLLETYRYTPGPPLDCPITALGGDDDPLVSPEELAAWREHTTGPFRLRVFPGGHFFLRDHPAELLALIRAALAA 235
Thioesterase pfam00975
Thioesterase domain; Peptide synthetases are involved in the non-ribosomal synthesis of ...
 25-249 1.15e-62

Thioesterase domain; Peptide synthetases are involved in the non-ribosomal synthesis of peptide antibiotics. Next to the operons encoding these enzymes, in almost all cases, are genes that encode proteins that have similarity to the type II fatty acid thioesterases of vertebrates. There are also modules within the peptide synthetases that also share this similarity. With respect to antibiotic production, thioesterases are required for the addition of the last amino acid to the peptide antibiotic, thereby forming a cyclic antibiotic. Thioesterases (non-integrated) have molecular masses of 25-29 kDa.


Pssm-ID: 395776 [Multi-domain]  Cd Length: 223  Bit Score: 195.68  E-value: 1.15e-62
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141   25 RLVCFPYAGGSGSFFLPISRALAPTVEVLAVQYPARQtrRLEPGIDNIPDFADQIFAAVRQS-TDKPLAFFGHSMGAVLA 103
Cdd:pfam00975   2 PLFCFPPAGGSASSFRSLARRLPPPAEVLAVQYPGRG--RGEPPLNSIEALADEYAEALRQIqPEGPYALFGHSMGGMLA 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141  104 YEVALRFQQAGLPsPVRLFVSGRRAPSKYRDERFHAAPDAEFIAELKGLGGPNSAFLNDPEMLEMVLPALRSDYTAIERY 183
Cdd:pfam00975  80 FEVARRLERQGEA-VRSLFLSDASAPHTVRYEASRAPDDDEVVAEFTDEGGTPEELLEDEELLSMLLPALRADYRALESY 158

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 490391141  184 RHEPGRTLDcpISAFVGDSDPRASLDEVRAWA-QHTTGPFDLEIFQGDHFYLIGQGPQLIELIAKKL 249
Cdd:pfam00975 159 SCPPLDAQS--ATLFYGSDDPLHDADDLAEWVrDHTPGEFDVHVFDGDHFYLIEHLEAVLEIIEAKL 223
EntF2 COG3319
Thioesterase domain of type I polyketide synthase or non-ribosomal peptide synthetase ...
 26-250 6.03e-10

Thioesterase domain of type I polyketide synthase or non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 442548 [Multi-domain]  Cd Length: 855  Bit Score: 59.33  E-value: 6.03e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141  26 LVCFPYAGGSGSFFLPISRALAPTVEVLAVQYPARQTRrlEPGIDNIPDFADQIFAAVRQ-STDKPLAFFGHSMGAVLAY 104
Cdd:COG3319  604 LFCVHPAGGNVLCYRPLARALGPDRPVYGLQAPGLDGG--EPPPASVEEMAARYVEAIRAvQPEGPYHLLGWSFGGLVAY 681

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141 105 EVALRFQQAG------------LPSPVR-----------LFVSGRRAPSKYRDERFHAAPDAEFIAELKGLGGPNSAFLN 161
Cdd:COG3319  682 EMARQLEAQGeevallvlldsyAPGALArldeaellaalLRDLARGVDLPLDAEELRALDPEERLARLLERLREAGLPAG 761

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141 162 -DPEMLEMVLPALRSDYTAIERYRHEPgrtLDCPISAFV-GDSDPRASLDEVRAWAQHTTGPFDLEIFQGDHFYLIgqGP 239
Cdd:COG3319  762 lDAERLRRLLRVFRANLRALRRYRPRP---YDGPVLLFRaEEDPPGRADDPALGWRPLVAGGLEVHDVPGDHFSML--RE 836

                        250
                 ....*....|.
gi 490391141 240 QLIELIAKKLA 250
Cdd:COG3319  837 PHVAELAAALR 847
MenH COG0596
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase MenH and related esterases, ...
 33-246 9.61e-07

2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase MenH and related esterases, alpha/beta hydrolase fold [Coenzyme transport and metabolism, General function prediction only]; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase MenH and related esterases, alpha/beta hydrolase fold is part of the Pathway/BioSystem: Menaquinone biosynthesis


Pssm-ID: 440361 [Multi-domain]  Cd Length: 221  Bit Score: 48.07  E-value: 9.61e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141  33 GGSGSFFLPISRALAPTVEVLAVQYPARQTRRLEPGIDNIPDFADQIFAAVRQSTDKPLAFFGHSMGAVLAYEVALRFqq 112
Cdd:COG0596   33 PGSSYEWRPLIPALAAGYRVIAPDLRGHGRSDKPAGGYTLDDLADDLAALLDALGLERVVLVGHSMGGMVALELAARH-- 110

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141 113 aglPSPVRLFVsgrrapskyrderfhaapdaeFIAELKglggpnSAFLNDPEMLEMVLPALRSDYTAIERYRHEPG-RTL 191
Cdd:COG0596  111 ---PERVAGLV---------------------LVDEVL------AALAEPLRRPGLAPEALAALLRALARTDLRERlARI 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 490391141 192 DCPISAFVGDSDPRASLDEVRAWAQHTTGpFDLEIFQG-DHFYLIGQGPQLIELIA 246
Cdd:COG0596  161 TVPTLVIWGEKDPIVPPALARRLAELLPN-AELVVLPGaGHFPPLEQPEAFAAALR 215
PldB COG2267
Lysophospholipase, alpha-beta hydrolase superfamily [Lipid transport and metabolism];
63-232 5.80e-05

Lysophospholipase, alpha-beta hydrolase superfamily [Lipid transport and metabolism];


Pssm-ID: 441868 [Multi-domain]  Cd Length: 221  Bit Score: 43.07  E-value: 5.80e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141  63 RRLEPGIDNIPDFADQIFAAVRQSTDKPLAFFGHSMGAVLAYEVALRFqqaglPSPVRLFVsgrrapskyrderfhaapd 142
Cdd:COG2267   73 RGHVDSFDDYVDDLRAALDALRARPGLPVVLLGHSMGGLIALLYAARY-----PDRVAGLV------------------- 128

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141 143 aefiaelkgLGGPnsAFLNDPEMLemVLPALRSDYTAIERYRHepgrtLDCPISAFVGDSDPRASLDEVRAWAQHTTGPF 222
Cdd:COG2267  129 ---------LLAP--AYRADPLLG--PSARWLRALRLAEALAR-----IDVPVLVLHGGADRVVPPEAARRLAARLSPDV 190

                        170
                 ....*....|.
gi 490391141 223 DLEIFQG-DHF 232
Cdd:COG2267  191 ELVLLPGaRHE 201
Abhydrolase_6 pfam12697
Alpha/beta hydrolase family; This family contains alpha/beta hydrolase enzymes of diverse ...
 34-232 9.00e-05

Alpha/beta hydrolase family; This family contains alpha/beta hydrolase enzymes of diverse specificity.


Pssm-ID: 463673 [Multi-domain]  Cd Length: 211  Bit Score: 42.46  E-value: 9.00e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141   34 GSGSFFLPISRALAPTVEVLAVQYPARQTRRLEPgiDNIPDFADQIFAAVRQSTDKPLAFFGHSMGAVLAYEValrfqqA 113
Cdd:pfam12697   6 GAGLSAAPLAALLAAGVAVLAPDLPGHGSSSPPP--LDLADLADLAALLDELGAARPVVLVGHSLGGAVALAA------A 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141  114 GLPSPVRLFVSGRRAPSKYRDERFHAAPDAEFIAELKGLGGPNS---AFLNDPEMLEMV---LPALRSDYTAIERYRHEP 187
Cdd:pfam12697  78 AAALVVGVLVAPLAAPPGLLAALLALLARLGAALAAPAWLAAESlarGFLDDLPADAEWaaaLARLAALLAALALLPLAA 157

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*
gi 490391141  188 GRTLDCPIsAFVGDSDPRASLDEVRAWAQHTTGPFDlEIFQGDHF 232
Cdd:pfam12697 158 WRDLPVPV-LVLAEEDRLVPELAQRLLAALAGARLV-VLPGAGHL 200
Abhydrolase_1 pfam00561
alpha/beta hydrolase fold; This catalytic domain is found in a very wide range of enzymes.
 26-232 1.18e-04

alpha/beta hydrolase fold; This catalytic domain is found in a very wide range of enzymes.


Pssm-ID: 395444 [Multi-domain]  Cd Length: 245  Bit Score: 42.11  E-value: 1.18e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141   26 LVCFPYAGGSGSFFLPISRALAPTV-EVLAVQYP--ARQTRRLEPGIDNIPDFADQIFAAVRQSTDKPLAFFGHSMGAVL 102
Cdd:pfam00561   3 VLLLHGLPGSSDLWRKLAPALARDGfRVIALDLRgfGKSSRPKAQDDYRTDDLAEDLEYILEALGLEKVNLVGHSMGGLI 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141  103 AYEVALRFQQ--------AGLPSPV---RLFVSGRRAPSKYRD---ERFHAAPDAEFIAELKGLGGPNSAFLNdpEMLEM 168
Cdd:pfam00561  83 ALAYAAKYPDrvkalvllGALDPPHeldEADRFILALFPGFFDgfvADFAPNPLGRLVAKLLALLLLRLRLLK--ALPLL 160

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 490391141  169 VLPALRSDY---------------TAIERYRHEPGRTLDCPISAFVGDSDPRASLDEVRAWAQHTTGPFDLEIFQGDHF 232
Cdd:pfam00561 161 NKRFPSGDYalakslvtgallfieTWSTELRAKFLGRLDEPTLIIWGDQDPLVPPQALEKLAQLFPNARLVVIPDAGHF 239
PKS_TE smart00824
Thioesterase; Peptide synthetases are involved in the non-ribosomal synthesis of peptide ...
 81-235 4.42e-04

Thioesterase; Peptide synthetases are involved in the non-ribosomal synthesis of peptide antibiotics. Next to the operons encoding these enzymes, in almost all cases, are genes that encode proteins that have similarity to the type II fatty acid thioesterases of vertebrates. There are also modules within the peptide synthetases that also share this similarity. With respect to antibiotic production, thioesterases are required for the addition of the last amino acid to the peptide antibiotic, thereby forming a cyclic antibiotic. Thioesterases (non-integrated) have molecular masses of 25-29 kDa.


Pssm-ID: 214835 [Multi-domain]  Cd Length: 212  Bit Score: 40.29  E-value: 4.42e-04
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141    81 AAVRQSTDKPLAFFGHSMGAVLAYEVALRFQQAGL-PSPVRLFVSgrRAPSKYRDERFHAAPDAEFIAELKGLGGPNSAF 159
Cdd:smart00824  56 AVLRAAGGRPFVLVGHSSGGLLAHAVAARLEARGIpPAAVVLLDT--YPPGDPAPEGWLPELLRGVFEREDSFVPMDDAR 133

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141   160 LndpemlemvlpalrsdyTAIERYRHEPGRTLDCPISA---FVGDSDP--RASLDEVRAWAQHTTGPFDLEIFQGDHFYL 234
Cdd:smart00824 134 L-----------------TAMGAYLRLFGGWTPGPVAAptlLVRASEPlaEWPDEDPDGWRAHWPLPHTVVDVPGDHFTM 196


                   .
gi 490391141   235 I 235
Cdd:smart00824 197 M 197
entF PRK10252
enterobactin non-ribosomal peptide synthetase EntF;
26-250 1.09e-03

enterobactin non-ribosomal peptide synthetase EntF;


Pssm-ID: 236668 [Multi-domain]  Cd Length: 1296  Bit Score: 40.03  E-value: 1.09e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141   26 LVCFPYAGGSGSFFLPISRALAPTVEVLAVQYPARQTRRLEPgiDNIPDFADQIFAAVRQSTDK-PLAFFGHSMGAVLAY 104
Cdd:PRK10252 1071 LFCFHPASGFAWQFSVLSRYLDPQWSIYGIQSPRPDGPMQTA--TSLDEVCEAHLATLLEQQPHgPYHLLGYSLGGTLAQ 1148

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141  105 EVALRFQQAG-----------LPSPVRLFvsgRRAPSKYRDERFHAAPDAE---FIAELKGLGGpnsaflndpemlEMVL 170
Cdd:PRK10252 1149 GIAARLRARGeevaflglldtWPPETQNW---REKEANGLDPEVLAEIDREreaFLAAQQGSLS------------TELF 1213

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141  171 PALRSDYT-AIERYRHEPGRTLDCPISAFVGDSDPRASLDEVRAWAQHTTGpfdLEIFQGD--HFYLIgqGPQLIELIAK 247
Cdd:PRK10252 1214 TTIEGNYAdAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPEQAWSPWIAE---LDVYRQDcaHVDII--SPEAFEKIGP 1288


                  ...
gi 490391141  248 KLA 250
Cdd:PRK10252 1289 ILR 1291
YvaK COG1647
Esterase/lipase [Secondary metabolites biosynthesis, transport and catabolism];
74-221 1.66e-03

Esterase/lipase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 441253 [Multi-domain]  Cd Length: 246  Bit Score: 38.77  E-value: 1.66e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141  74 DFADQIFAAVRQSTDKpLAFFGHSMGAVLAYEVALRFQQ-AGLpspvrLFVSgrrAPSKYRDERFHAAPDAEFIAELKGL 152
Cdd:COG1647   70 EDVEEAYEILKAGYDK-VIVIGLSMGGLLALLLAARYPDvAGL-----VLLS---PALKIDDPSAPLLPLLKYLARSLRG 140

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 490391141 153 GGPNsafLNDPEMLEMV-----LPALRSDYTAIERYRHEPGRtLDCPISAFVGDSDPRASLDEVRAWAQHTTGP 221
Cdd:COG1647  141 IGSD---IEDPEVAEYAydrtpLRALAELQRLIREVRRDLPK-ITAPTLIIQSRKDEVVPPESARYIYERLGSP 210
YpfH COG0400
Predicted esterase [General function prediction only];
26-113 2.00e-03

Predicted esterase [General function prediction only];


Pssm-ID: 440169 [Multi-domain]  Cd Length: 200  Bit Score: 38.35  E-value: 2.00e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141  26 LVCFPYAGGSGSFFLPISRALA-PTVEVLAVQ------YPARQ--TRRLEPGIDNIPDFA---DQIFAAVRQS------T 87
Cdd:COG0400    8 VVLLHGYGGDEEDLLPLAPELAlPGAAVLAPRapvpegPGGRAwfDLSFLEGREDEEGLAaaaEALAAFIDELearygiD 87

                         90       100
                 ....*....|....*....|....*.
gi 490391141  88 DKPLAFFGHSMGAVLAYEVALRFQQA 113
Cdd:COG0400   88 PERIVLAGFSQGAAMALSLALRRPEL 113
EstA COG1075
Triacylglycerol esterase/lipase EstA, alpha/beta hydrolase fold [Lipid transport and ...
 33-124 2.45e-03

Triacylglycerol esterase/lipase EstA, alpha/beta hydrolase fold [Lipid transport and metabolism];


Pssm-ID: 440693 [Multi-domain]  Cd Length: 106  Bit Score: 36.73  E-value: 2.45e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141  33 GGSGSFFLPISRAL-APTVEVLAVQYPARQtRRLEpgiDNIPDFADQIFAAVRQSTDKPLAFFGHSMGAVLAYEVAlrfQ 111
Cdd:COG1075   15 GGSAASWAPLAPRLrAAGYPVYALNYPSTN-GSIE---DSAEQLAAFVDAVLAATGAEKVDLVGHSMGGLVARYYL---K 87

                         90
                 ....*....|...
gi 490391141 112 QAGLPSPVRLFVS 124
Cdd:COG1075   88 RLGGAAKVARVVT 100
DAP2 COG1506
Dipeptidyl aminopeptidase/acylaminoacyl peptidase [Amino acid transport and metabolism];
14-246 6.45e-03

Dipeptidyl aminopeptidase/acylaminoacyl peptidase [Amino acid transport and metabolism];


Pssm-ID: 441115 [Multi-domain]  Cd Length: 234  Bit Score: 36.92  E-value: 6.45e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141  14 TVFTPAPQSSVRLVCFPYAGGSGSF--FLPISRALAptvE----VLAVQYPARQTRRLEPGIDNIPDFAdqifAAVRQST 87
Cdd:COG1506   13 WLYLPADGKKYPVVVYVHGGPGSRDdsFLPLAQALA---SrgyaVLAPDYRGYGESAGDWGGDEVDDVL----AAIDYLA 85

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141  88 DKP------LAFFGHSMGAVLAYEVALRfqqaglpspvrlfvsgrrapskyRDERFHA----APDAEFIAELKGLGGPNS 157
Cdd:COG1506   86 ARPyvdpdrIGIYGHSYGGYMALLAAAR-----------------------HPDRFKAavalAGVSDLRSYYGTTREYTE 142

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490391141 158 AFLNDPEMLEMVLPAlRSDYTAIERyrhepgrtLDCPISAFVGDSDPRASLDEVR---AWAQHTTGPFDLEIFQG-DHFY 233
Cdd:COG1506  143 RLMGGPWEDPEAYAA-RSPLAYADK--------LKTPLLLIHGEADDRVPPEQAErlyEALKKAGKPVELLVYPGeGHGF 213

                        250
                 ....*....|...
gi 490391141 234 LIGQGPQLIELIA 246
Cdd:COG1506  214 SGAGAPDYLERIL 226
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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