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Conserved domains on  [gi|490173398|ref|WP_004072035|]
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prepilin-type N-terminal cleavage/methylation domain-containing protein [Desulfobacter postgatei]

Protein Classification

PilV family protein( domain architecture ID 706969)

PilV family protein, similar to type IV pili (T4P) PilV protein; T4P, found on the surface of many Gram-negative and Gram-positive bacteria, facilitates biological functions important for bacterial pathogenicity, including twitching motility, DNA uptake, and adhesion to host cells

Gene Ontology:  GO:0044096
PubMed:  30988511|23204365

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PilV COG4967
Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];
3-60 1.11e-16

Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];


:

Pssm-ID: 443993 [Multi-domain]  Cd Length: 86  Bit Score: 70.01  E-value: 1.11e-16
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 490173398   3 FLRNNQGFTLIESLIAMFVLAVGILALNTMQISSIRGNTSANKLTVASTLAGNSYERL 60
Cdd:COG4967    6 RRRRQRGFTLIEVLVALVILSIGLLGLAGLQAASLRSSQDARQRTQAALLAQDLLERL 63
acid_disulf_rpt NF033662
acidic double-disulfide repeat; The acidic double-disulfide repeat is an Asp-rich repeat with ...
 99-119 5.77e-06

acidic double-disulfide repeat; The acidic double-disulfide repeat is an Asp-rich repeat with four nearly invariant Cys residues in a repeat length of about 35 amino acids.


:

Pssm-ID: 411265 [Multi-domain]  Cd Length: 32  Bit Score: 40.96  E-value: 5.77e-06
                         10        20
                 ....*....|....*....|.
gi 490173398  99 TEWTNSDGVDNDGDGVTDESD 119
Cdd:NF033662   3 TDTTCSDGIDNDGDGLTDCAD 23
 
Name Accession Description Interval E-value
PilV COG4967
Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];
3-60 1.11e-16

Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];


Pssm-ID: 443993 [Multi-domain]  Cd Length: 86  Bit Score: 70.01  E-value: 1.11e-16
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 490173398   3 FLRNNQGFTLIESLIAMFVLAVGILALNTMQISSIRGNTSANKLTVASTLAGNSYERL 60
Cdd:COG4967    6 RRRRQRGFTLIEVLVALVILSIGLLGLAGLQAASLRSSQDARQRTQAALLAQDLLERL 63
type_IV_pilV TIGR02523
type IV pilus modification protein PilV; Pilus systems categorized as type IV pilins differ ...
 9-61 4.55e-08

type IV pilus modification protein PilV; Pilus systems categorized as type IV pilins differ greatly from one another, with some showing greater similarty to type II or type III secretion systems than to each other. Members of this protein family represent the PilV protein of type IV pilus systems as found in Pseudomonas aeruginosa PAO1, Pseudomonas syringae DC3000, Neisseria meningitidis MC58, Xylella fastidiosa 9a5c, etc. [Cell envelope, Surface structures, Protein fate, Protein modification and repair]


Pssm-ID: 131575  Cd Length: 139  Bit Score: 48.99  E-value: 4.55e-08
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 490173398    9 GFTLIESLIAMFVLAVGILALNTMQISSIRGNTSANKLTVASTLAGNSYERLL 61
Cdd:TIGR02523   3 GFSMIEVLVALLVLAIGVLGMAALQLKAVRYTRSASTRTIASMLAYNLLDRMR 55
acid_disulf_rpt NF033662
acidic double-disulfide repeat; The acidic double-disulfide repeat is an Asp-rich repeat with ...
 99-119 5.77e-06

acidic double-disulfide repeat; The acidic double-disulfide repeat is an Asp-rich repeat with four nearly invariant Cys residues in a repeat length of about 35 amino acids.


Pssm-ID: 411265 [Multi-domain]  Cd Length: 32  Bit Score: 40.96  E-value: 5.77e-06
                         10        20
                 ....*....|....*....|.
gi 490173398  99 TEWTNSDGVDNDGDGVTDESD 119
Cdd:NF033662   3 TDTTCSDGIDNDGDGLTDCAD 23
N_methyl pfam07963
Prokaryotic N-terminal methylation motif; This short motif directs methylation of the ...
 5-29 1.19e-05

Prokaryotic N-terminal methylation motif; This short motif directs methylation of the conserved phenylalanine residue. It is most often found at the N-terminus of pilins and other proteins involved in secretion, see pfam00114, pfam05946, pfam02501 and pfam07596.


Pssm-ID: 429756 [Multi-domain]  Cd Length: 27  Bit Score: 40.05  E-value: 1.19e-05
                          10        20
                  ....*....|....*....|....*
gi 490173398    5 RNNQGFTLIESLIAMFVLAVGILAL 29
Cdd:pfam07963   2 RKQRGFTLIELLVALAILAILLAAA 26
T4P_ComGE NF041013
competence type IV pilus minor pilin ComGE;
5-24 1.20e-03

competence type IV pilus minor pilin ComGE;


Pssm-ID: 468942 [Multi-domain]  Cd Length: 83  Bit Score: 36.02  E-value: 1.20e-03
                         10        20
                 ....*....|....*....|
gi 490173398   5 RNNQGFTLIESLIAMFVLAV 24
Cdd:NF041013   1 KKNKGFILLESLVALALLAL 20
 
Name Accession Description Interval E-value
PilV COG4967
Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];
3-60 1.11e-16

Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];


Pssm-ID: 443993 [Multi-domain]  Cd Length: 86  Bit Score: 70.01  E-value: 1.11e-16
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 490173398   3 FLRNNQGFTLIESLIAMFVLAVGILALNTMQISSIRGNTSANKLTVASTLAGNSYERL 60
Cdd:COG4967    6 RRRRQRGFTLIEVLVALVILSIGLLGLAGLQAASLRSSQDARQRTQAALLAQDLLERL 63
type_IV_pilV TIGR02523
type IV pilus modification protein PilV; Pilus systems categorized as type IV pilins differ ...
 9-61 4.55e-08

type IV pilus modification protein PilV; Pilus systems categorized as type IV pilins differ greatly from one another, with some showing greater similarty to type II or type III secretion systems than to each other. Members of this protein family represent the PilV protein of type IV pilus systems as found in Pseudomonas aeruginosa PAO1, Pseudomonas syringae DC3000, Neisseria meningitidis MC58, Xylella fastidiosa 9a5c, etc. [Cell envelope, Surface structures, Protein fate, Protein modification and repair]


Pssm-ID: 131575  Cd Length: 139  Bit Score: 48.99  E-value: 4.55e-08
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 490173398    9 GFTLIESLIAMFVLAVGILALNTMQISSIRGNTSANKLTVASTLAGNSYERLL 61
Cdd:TIGR02523   3 GFSMIEVLVALLVLAIGVLGMAALQLKAVRYTRSASTRTIASMLAYNLLDRMR 55
acid_disulf_rpt NF033662
acidic double-disulfide repeat; The acidic double-disulfide repeat is an Asp-rich repeat with ...
 99-119 5.77e-06

acidic double-disulfide repeat; The acidic double-disulfide repeat is an Asp-rich repeat with four nearly invariant Cys residues in a repeat length of about 35 amino acids.


Pssm-ID: 411265 [Multi-domain]  Cd Length: 32  Bit Score: 40.96  E-value: 5.77e-06
                         10        20
                 ....*....|....*....|.
gi 490173398  99 TEWTNSDGVDNDGDGVTDESD 119
Cdd:NF033662   3 TDTTCSDGIDNDGDGLTDCAD 23
N_methyl pfam07963
Prokaryotic N-terminal methylation motif; This short motif directs methylation of the ...
 5-29 1.19e-05

Prokaryotic N-terminal methylation motif; This short motif directs methylation of the conserved phenylalanine residue. It is most often found at the N-terminus of pilins and other proteins involved in secretion, see pfam00114, pfam05946, pfam02501 and pfam07596.


Pssm-ID: 429756 [Multi-domain]  Cd Length: 27  Bit Score: 40.05  E-value: 1.19e-05
                          10        20
                  ....*....|....*....|....*
gi 490173398    5 RNNQGFTLIESLIAMFVLAVGILAL 29
Cdd:pfam07963   2 RKQRGFTLIELLVALAILAILLAAA 26
PulG COG2165
Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, ...
 1-75 2.34e-05

Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, secretion, and vesicular transport, Extracellular structures];


Pssm-ID: 441768 [Multi-domain]  Cd Length: 99  Bit Score: 41.05  E-value: 2.34e-05
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 490173398   1 MTFLRNNQGFTLIESLIAMFVLAVGILALntmqISSIRGNTSANKLTVASTLAgNSYERLLNVSYNDSTMDPATN 75
Cdd:COG2165    4 RRRRRRQRGFTLIELLVVIAIIGILAALA----LPALQGARERARRAELRSNL-RQIQQALERYRLDNGRYPSSL 73
PulJ COG4795
Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and ...
 1-43 3.16e-04

Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 443823 [Multi-domain]  Cd Length: 118  Bit Score: 38.07  E-value: 3.16e-04
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 490173398   1 MTFLRNNQ-GFTLIESLIAMFVLAVGILALNTMQISSIRGNTSA 43
Cdd:COG4795    1 MKRARRRQrGFTLLELLVALAIFALLLLAAYRGLDSVLRSRERL 44
FimT COG4970
Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];
5-53 3.61e-04

Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];


Pssm-ID: 443996 [Multi-domain]  Cd Length: 73  Bit Score: 37.13  E-value: 3.61e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 490173398   5 RNNQGFTLIESLIAMFVLA----VGILALNTMqISSIRGNTSANKLTVASTLA 53
Cdd:COG4970    6 RRQRGFTLIELLVVLAILAilaaIAVPSFSSL-IARQRLRAAANELAAALRLA 57
T4P_ComGE NF041013
competence type IV pilus minor pilin ComGE;
5-24 1.20e-03

competence type IV pilus minor pilin ComGE;


Pssm-ID: 468942 [Multi-domain]  Cd Length: 83  Bit Score: 36.02  E-value: 1.20e-03
                         10        20
                 ....*....|....*....|
gi 490173398   5 RNNQGFTLIESLIAMFVLAV 24
Cdd:NF041013   1 KKNKGFILLESLVALALLAL 20
ComGF COG4940
Competence protein ComGF [Mobilome: prophages, transposons];
2-45 2.00e-03

Competence protein ComGF [Mobilome: prophages, transposons];


Pssm-ID: 443967 [Multi-domain]  Cd Length: 153  Bit Score: 36.53  E-value: 2.00e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*.
gi 490173398   2 TFLRNNQGFTLIESLIAMFVLA--VGILALNTMQISSIRGNTSANK 45
Cdd:COG4940   10 AASINRKGFTLLEKLAALMIIAyiLAVLELLLKLLLKLNQSLDAGE 55
IV_pilin_GFxxxE TIGR02532
prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all ...
 7-29 3.04e-03

prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all examples of the N-terminal region of bacterial proteins that resemble type IV pilins at their N-terminus, with a cleavage site G^FxxxE followed by a hydrophobic stretch. The new N-terminal residue, usually Phe, is methylated. Separate domains of the prepilin peptidase appear responsible for cleavage and methylation. Proteins with this N-terminal region include type IV pilins and other components of pilus biogenesis, competence proteins, and type II secretion proteins. Typically several proteins in a single operon have this N-terminal domain. The N-terminal cleavage and methylation site is described by PROSITE motif PS00409 as [KRHEQSTAG]-G-[FYLIVM]-[ST]-[LT]-[LIVP]-E-[LIVMFWSTAG](14). [Cell envelope, Surface structures, Protein fate, Protein and peptide secretion and trafficking]


Pssm-ID: 274182 [Multi-domain]  Cd Length: 24  Bit Score: 33.43  E-value: 3.04e-03
                          10        20
                  ....*....|....*....|...
gi 490173398    7 NQGFTLIESLIAMFVLAVGILAL 29
Cdd:TIGR02532   1 QRGFTLIELLVVLAILGILALIA 23
ComGC COG4537
Competence protein ComGC [Mobilome: prophages, transposons];
3-27 3.74e-03

Competence protein ComGC [Mobilome: prophages, transposons];


Pssm-ID: 443603 [Multi-domain]  Cd Length: 108  Bit Score: 34.90  E-value: 3.74e-03
                         10        20
                 ....*....|....*....|....*
gi 490173398   3 FLRNNQGFTLIESLIAMFVLAVGIL 27
Cdd:COG4537    7 KLKKEKGFTLIEMLIVLLIISILLL 31
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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