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Conserved domains on  [gi|489276232|ref|WP_003183920|]
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MULTISPECIES: IreB family regulatory phosphoprotein [Bacillus]

Protein Classification

IreB family regulatory phosphoprotein( domain architecture ID 10012429)

IreB family regulatory phosphoprotein similar to IreB, which was characterized in Enterococcus faecalis as a small protein, well-conserved in the Firmicutes. It belongs to a system that includes the Ser/Thr protein kinase IreK, and phosphatase IreP, undergoes phosphorylation on threonine residues, and is involved in regulating cephalosporin resistance. This family was previously named DUF965

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
IreB-like PRK05473
IreB family regulatory phosphoprotein; IreB (EF1202) was characterized in Enterococcus ...
 1-87 2.12e-58

IreB family regulatory phosphoprotein; IreB (EF1202) was characterized in Enterococcus faecalis as a small protein, well-conserved in the Firmicutes. It belongs to a system that includes the Ser/Thr protein kinase IreK, and phosphatase IreP, undergoes phosphorylation on threonine residues, and is involved in regulating cephalosporin resistance. This family was previously named DUF965 by Pfam model pfam06135


:

Pssm-ID: 180108  Cd Length: 86  Bit Score: 173.09  E-value: 2.12e-58
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489276232  1 MSSFDKTMKFNFSDDsMETNVNEVLITVHDALQEKGYNPINQIVGYLLSGDPAYIPRHRDARNLIRKIERDEIIEELVKS 80
Cdd:PRK05473  1 MSSFDKTMRFDFDDE-KKKDVREILTTVYDALEEKGYNPINQIVGYLLSGDPAYIPRHNDARNLIRKLERDEILEELVKS 79


                ....*..
gi 489276232 81 YLEQHKE 87
Cdd:PRK05473 80 YLKGNGK 86
 
Name Accession Description Interval E-value
IreB-like PRK05473
IreB family regulatory phosphoprotein; IreB (EF1202) was characterized in Enterococcus ...
 1-87 2.12e-58

IreB family regulatory phosphoprotein; IreB (EF1202) was characterized in Enterococcus faecalis as a small protein, well-conserved in the Firmicutes. It belongs to a system that includes the Ser/Thr protein kinase IreK, and phosphatase IreP, undergoes phosphorylation on threonine residues, and is involved in regulating cephalosporin resistance. This family was previously named DUF965 by Pfam model pfam06135


Pssm-ID: 180108  Cd Length: 86  Bit Score: 173.09  E-value: 2.12e-58
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489276232  1 MSSFDKTMKFNFSDDsMETNVNEVLITVHDALQEKGYNPINQIVGYLLSGDPAYIPRHRDARNLIRKIERDEIIEELVKS 80
Cdd:PRK05473  1 MSSFDKTMRFDFDDE-KKKDVREILTTVYDALEEKGYNPINQIVGYLLSGDPAYIPRHNDARNLIRKLERDEILEELVKS 79


                ....*..
gi 489276232 81 YLEQHKE 87
Cdd:PRK05473 80 YLKGNGK 86
COG4472 COG4472
Uncharacterized conserved protein, UPF0297 family [Function unknown];
5-86 3.20e-53

Uncharacterized conserved protein, UPF0297 family [Function unknown];


Pssm-ID: 443569  Cd Length: 81  Bit Score: 159.93  E-value: 3.20e-53
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489276232  5 DKTMKFNFsDDSMETNVNEVLITVHDALQEKGYNPINQIVGYLLSGDPAYIPRHRDARNLIRKIERDEIIEELVKSYLEQ 84
Cdd:COG4472   1 DETMKFNF-DKEEEEDVKEILTEVYEALEEKGYNPINQIVGYLLSGDPAYITSHNNARNLIRKLERDELLEELVKSYLKE 79


                ..
gi 489276232 85 HK 86
Cdd:COG4472  80 KG 81
IreB pfam06135
IreB regulatory phosphoprotein; IreB is a cytosolic 10.5-kDa protein that is highly conserved ...
 5-82 1.10e-50

IreB regulatory phosphoprotein; IreB is a cytosolic 10.5-kDa protein that is highly conserved among low-GC Gram-positive bacteria. Studies of E. faecalis mutants lacking ireB revealed that IreB acts downstream of IreK as a negative regulator of cephalosporin resistance in E. faecalis. IreB can be modified by phosphorylation in vivo, and mutations that prevent modification at the known sites of phosphorylation (T4 and T7) influence the ability of IreB to modulate cephalosporin resistance. Furthermore, mutations that impair dimerization abolish the regulation of cephalosporin resistance.


Pssm-ID: 428785  Cd Length: 77  Bit Score: 153.35  E-value: 1.10e-50
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 489276232   5 DKTMKFNFSDDSmETNVNEVLITVHDALQEKGYNPINQIVGYLLSGDPAYIPRHRDARNLIRKIERDEIIEELVKSYL 82
Cdd:pfam06135  1 DETMKFKVEKEK-EEEVKEILETVYEALEEKGYNPINQIVGYLLSGDPTYITSHNNARSLIRKIERDELLEELVKSYL 77
 
Name Accession Description Interval E-value
IreB-like PRK05473
IreB family regulatory phosphoprotein; IreB (EF1202) was characterized in Enterococcus ...
 1-87 2.12e-58

IreB family regulatory phosphoprotein; IreB (EF1202) was characterized in Enterococcus faecalis as a small protein, well-conserved in the Firmicutes. It belongs to a system that includes the Ser/Thr protein kinase IreK, and phosphatase IreP, undergoes phosphorylation on threonine residues, and is involved in regulating cephalosporin resistance. This family was previously named DUF965 by Pfam model pfam06135


Pssm-ID: 180108  Cd Length: 86  Bit Score: 173.09  E-value: 2.12e-58
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489276232  1 MSSFDKTMKFNFSDDsMETNVNEVLITVHDALQEKGYNPINQIVGYLLSGDPAYIPRHRDARNLIRKIERDEIIEELVKS 80
Cdd:PRK05473  1 MSSFDKTMRFDFDDE-KKKDVREILTTVYDALEEKGYNPINQIVGYLLSGDPAYIPRHNDARNLIRKLERDEILEELVKS 79


                ....*..
gi 489276232 81 YLEQHKE 87
Cdd:PRK05473 80 YLKGNGK 86
COG4472 COG4472
Uncharacterized conserved protein, UPF0297 family [Function unknown];
5-86 3.20e-53

Uncharacterized conserved protein, UPF0297 family [Function unknown];


Pssm-ID: 443569  Cd Length: 81  Bit Score: 159.93  E-value: 3.20e-53
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489276232  5 DKTMKFNFsDDSMETNVNEVLITVHDALQEKGYNPINQIVGYLLSGDPAYIPRHRDARNLIRKIERDEIIEELVKSYLEQ 84
Cdd:COG4472   1 DETMKFNF-DKEEEEDVKEILTEVYEALEEKGYNPINQIVGYLLSGDPAYITSHNNARNLIRKLERDELLEELVKSYLKE 79


                ..
gi 489276232 85 HK 86
Cdd:COG4472  80 KG 81
IreB pfam06135
IreB regulatory phosphoprotein; IreB is a cytosolic 10.5-kDa protein that is highly conserved ...
 5-82 1.10e-50

IreB regulatory phosphoprotein; IreB is a cytosolic 10.5-kDa protein that is highly conserved among low-GC Gram-positive bacteria. Studies of E. faecalis mutants lacking ireB revealed that IreB acts downstream of IreK as a negative regulator of cephalosporin resistance in E. faecalis. IreB can be modified by phosphorylation in vivo, and mutations that prevent modification at the known sites of phosphorylation (T4 and T7) influence the ability of IreB to modulate cephalosporin resistance. Furthermore, mutations that impair dimerization abolish the regulation of cephalosporin resistance.


Pssm-ID: 428785  Cd Length: 77  Bit Score: 153.35  E-value: 1.10e-50
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 489276232   5 DKTMKFNFSDDSmETNVNEVLITVHDALQEKGYNPINQIVGYLLSGDPAYIPRHRDARNLIRKIERDEIIEELVKSYL 82
Cdd:pfam06135  1 DETMKFKVEKEK-EEEVKEILETVYEALEEKGYNPINQIVGYLLSGDPTYITSHNNARSLIRKIERDELLEELVKSYL 77
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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