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Conserved domains on  [gi|489176367|ref|WP_003085889|]
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MULTISPECIES: lysozyme inhibitor [Pseudomonas]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK11372 super family cl01604
C-type lysozyme inhibitor;
1-127 7.36e-96

C-type lysozyme inhibitor;


The actual alignment was detected with superfamily member PRK13792:

Pssm-ID: 470265  Cd Length: 127  Bit Score: 271.20  E-value: 7.36e-96
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489176367   1 MKKALWLLLAAVPVVLVACGGSDDDKQTAQVDYLALPGDAKLDTRSVDYKCENGRKFTVQYLNKGDNSLAVVPVSDNSTL 80
Cdd:PRK13792   1 MKKALWLLLAAVPVVLVACGGSDDDKQTAQVDYLALPGDAKLDTRSVDYKCENGRKFTVQYLNKGDNSLAVVPVSDNSTL 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 489176367  81 VFSNVISASGAKYAAGQYIWWTKGEEATLYGDWKGGEPTDGVACKER 127
Cdd:PRK13792  81 VFSNVISASGAKYAAGQYIWWTKGEEATLYGDWKGGEPTDGVACKER 127
 
Name Accession Description Interval E-value
PRK13792 PRK13792
lysozyme inhibitor; Provisional
 1-127 7.36e-96

lysozyme inhibitor; Provisional


Pssm-ID: 106733  Cd Length: 127  Bit Score: 271.20  E-value: 7.36e-96
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489176367   1 MKKALWLLLAAVPVVLVACGGSDDDKQTAQVDYLALPGDAKLDTRSVDYKCENGRKFTVQYLNKGDNSLAVVPVSDNSTL 80
Cdd:PRK13792   1 MKKALWLLLAAVPVVLVACGGSDDDKQTAQVDYLALPGDAKLDTRSVDYKCENGRKFTVQYLNKGDNSLAVVPVSDNSTL 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 489176367  81 VFSNVISASGAKYAAGQYIWWTKGEEATLYGDWKGGEPTDGVACKER 127
Cdd:PRK13792  81 VFSNVISASGAKYAAGQYIWWTKGEEATLYGDWKGGEPTDGVACKER 127
MliC COG3895
Membrane-bound inhibitor of C-type lysozyme [Cell wall/membrane/envelope biogenesis];
1-127 1.88e-33

Membrane-bound inhibitor of C-type lysozyme [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 443102  Cd Length: 108  Bit Score: 112.84  E-value: 1.88e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489176367   1 MKKalwLLLAAVPVVLVACGgsdddkqtaqvdYLALPGDAKLDTRSVDYKCENGRKFTVQYLNkGDNSLAVVPVsDNSTL 80
Cdd:COG3895    1 MKK---LLLLLAALLLAGCA------------SAAPPADPAPATQTVHYQCEDGKPLTVTYIN-NDNSLAVLRL-DGETL 63

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 489176367  81 VFSNVISASGAKYAAGQYIWWTKGEEATLYGDWKGGEPTDgvaCKER 127
Cdd:COG3895   64 VLKQVVSASGARYSDGQYVFWSKGDEATLERNGDDVVLND---CKLV 107
MliC pfam09864
Membrane-bound lysozyme-inhibitor of c-type lysozyme; Lysozymes are ancient and important ...
 49-120 3.66e-20

Membrane-bound lysozyme-inhibitor of c-type lysozyme; Lysozymes are ancient and important components of the innate immune system of animals that hydrolyse peptidoglycan, the major bacterial cell wall polymer. Various mechanisms have evolved by which bacteria can evade this bactericidal enzyme, one being the production of lysozyme inhibitors. MliC (membrane bound lysozyme inhibitor of c-type lysozyme) of E. coli and Pseudomonas aeruginosa, possess lysozyme inhibitory activity and confer increased lysozyme tolerance upon expression in E. coli. Structural analyses show that the invariant loop of MliC plays a crucial role in the inhibition of the lysozyme by its insertion into the active site cleft of the lysozyme, where the loop forms hydrogen and ionic bonds with the catalytic residues.


Pssm-ID: 462921  Cd Length: 68  Bit Score: 77.76  E-value: 3.66e-20
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 489176367   49 YKCENGRKFTVQYlNKGDNSLAVVPVsDNSTLVFSNVISASGAKYAAGQYIWWTKGEEATLYgdWKGGEPTD 120
Cdd:pfam09864   1 YQCDDGEEVTVTY-FDADPSTATLRL-GGEQLVLPQVPSASGARYVGGGYEWWTKGNEATLT--WGGKDPLQ 68
 
Name Accession Description Interval E-value
PRK13792 PRK13792
lysozyme inhibitor; Provisional
 1-127 7.36e-96

lysozyme inhibitor; Provisional


Pssm-ID: 106733  Cd Length: 127  Bit Score: 271.20  E-value: 7.36e-96
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489176367   1 MKKALWLLLAAVPVVLVACGGSDDDKQTAQVDYLALPGDAKLDTRSVDYKCENGRKFTVQYLNKGDNSLAVVPVSDNSTL 80
Cdd:PRK13792   1 MKKALWLLLAAVPVVLVACGGSDDDKQTAQVDYLALPGDAKLDTRSVDYKCENGRKFTVQYLNKGDNSLAVVPVSDNSTL 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 489176367  81 VFSNVISASGAKYAAGQYIWWTKGEEATLYGDWKGGEPTDGVACKER 127
Cdd:PRK13792  81 VFSNVISASGAKYAAGQYIWWTKGEEATLYGDWKGGEPTDGVACKER 127
MliC COG3895
Membrane-bound inhibitor of C-type lysozyme [Cell wall/membrane/envelope biogenesis];
1-127 1.88e-33

Membrane-bound inhibitor of C-type lysozyme [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 443102  Cd Length: 108  Bit Score: 112.84  E-value: 1.88e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489176367   1 MKKalwLLLAAVPVVLVACGgsdddkqtaqvdYLALPGDAKLDTRSVDYKCENGRKFTVQYLNkGDNSLAVVPVsDNSTL 80
Cdd:COG3895    1 MKK---LLLLLAALLLAGCA------------SAAPPADPAPATQTVHYQCEDGKPLTVTYIN-NDNSLAVLRL-DGETL 63

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 489176367  81 VFSNVISASGAKYAAGQYIWWTKGEEATLYGDWKGGEPTDgvaCKER 127
Cdd:COG3895   64 VLKQVVSASGARYSDGQYVFWSKGDEATLERNGDDVVLND---CKLV 107
MliC pfam09864
Membrane-bound lysozyme-inhibitor of c-type lysozyme; Lysozymes are ancient and important ...
 49-120 3.66e-20

Membrane-bound lysozyme-inhibitor of c-type lysozyme; Lysozymes are ancient and important components of the innate immune system of animals that hydrolyse peptidoglycan, the major bacterial cell wall polymer. Various mechanisms have evolved by which bacteria can evade this bactericidal enzyme, one being the production of lysozyme inhibitors. MliC (membrane bound lysozyme inhibitor of c-type lysozyme) of E. coli and Pseudomonas aeruginosa, possess lysozyme inhibitory activity and confer increased lysozyme tolerance upon expression in E. coli. Structural analyses show that the invariant loop of MliC plays a crucial role in the inhibition of the lysozyme by its insertion into the active site cleft of the lysozyme, where the loop forms hydrogen and ionic bonds with the catalytic residues.


Pssm-ID: 462921  Cd Length: 68  Bit Score: 77.76  E-value: 3.66e-20
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 489176367   49 YKCENGRKFTVQYlNKGDNSLAVVPVsDNSTLVFSNVISASGAKYAAGQYIWWTKGEEATLYgdWKGGEPTD 120
Cdd:pfam09864   1 YQCDDGEEVTVTY-FDADPSTATLRL-GGEQLVLPQVPSASGARYVGGGYEWWTKGNEATLT--WGGKDPLQ 68
PRK11372 PRK11372
C-type lysozyme inhibitor;
1-110 4.21e-13

C-type lysozyme inhibitor;


Pssm-ID: 236902  Cd Length: 109  Bit Score: 60.63  E-value: 4.21e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489176367   1 MKKalwLLLAAVPVVLVACGGSDDDKQTAQVDYLalpgdakldtrsvDYKCENgRKFTVQyLNKGDNSLAVVpvSDNSTL 80
Cdd:PRK11372   3 MKK---LLIICLPVLLTGCSAYNQFVERMQTDTL-------------EYQCDE-KPLTVK-LNNPRQEVSFV--YDNQLL 62

                         90       100       110
                 ....*....|....*....|....*....|
gi 489176367  81 VFSNVISASGAKYAAGQYIWWTKGEEATLY 110
Cdd:PRK11372  63 HLKQGISASGARYTDGIYVFWSKGDEATVY 92
CamS COG4851
Uncharacterized conserved protein involved in sex pheromone biosynthesis [General function ...
 1-30 3.38e-03

Uncharacterized conserved protein involved in sex pheromone biosynthesis [General function prediction only];


Pssm-ID: 443879 [Multi-domain]  Cd Length: 384  Bit Score: 36.03  E-value: 3.38e-03
                         10        20        30
                 ....*....|....*....|....*....|.
gi 489176367   1 MKKALWLLLAAVPVVLVACG-GSDDDKQTAQ 30
Cdd:COG4851    1 MKKILWILLLASLLLLSGCGpSFDKEEEVVQ 31
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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