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4-oxalocrotonate tautomerase [Streptococcus pyogenes]

Protein Classification

4-oxalocrotonate tautomerase family protein( domain architecture ID 10011791)

4-oxalocrotonate tautomerase family protein similar to 4-oxalocrotonate tautomerase, also called 2-hydroxymuconate tautomerase, which catalyzes the ketonization of 2-hydroxymuconate stereoselectively to yield 2-oxo-3-hexenedioate

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK02289 PRK02289
4-oxalocrotonate tautomerase; Provisional
 1-60 1.22e-37

4-oxalocrotonate tautomerase; Provisional


:

Pssm-ID: 179406  Cd Length: 60  Bit Score: 118.88  E-value: 1.22e-37
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 489074719  1 MPFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFINDMPEGTYYPQGEMKQK 60
Cdd:PRK02289  1 MPFVRIDLFEGRSQEQKNALAREVTEVVSRIAKAPKEAIHVFINDMPEGTYYPQGEMKTK 60
 
Name Accession Description Interval E-value
PRK02289 PRK02289
4-oxalocrotonate tautomerase; Provisional
 1-60 1.22e-37

4-oxalocrotonate tautomerase; Provisional


Pssm-ID: 179406  Cd Length: 60  Bit Score: 118.88  E-value: 1.22e-37
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 489074719  1 MPFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFINDMPEGTYYPQGEMKQK 60
Cdd:PRK02289  1 MPFVRIDLFEGRSQEQKNALAREVTEVVSRIAKAPKEAIHVFINDMPEGTYYPQGEMKTK 60
Tautomerase pfam01361
Tautomerase enzyme; This family includes the enzyme 4-oxalocrotonate tautomerase that ...
 2-58 9.07e-23

Tautomerase enzyme; This family includes the enzyme 4-oxalocrotonate tautomerase that catalyzes the ketonization of 2-hydroxymuconate to 2-oxo-3-hexenedioate.


Pssm-ID: 396090  Cd Length: 60  Bit Score: 81.18  E-value: 9.07e-23
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 489074719   2 PFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFINDMPEGTYYPQGEMK 58
Cdd:pfam01361  1 PFVNIKLFEGRTDEQKKELIERVTDALVEVLGAPRSAIVVIIEEVPPENWGVGGESV 57
PptA COG1942
Phenylpyruvate tautomerase PptA, 4-oxalocrotonate tautomerase family [Secondary metabolites ...
 2-57 2.01e-18

Phenylpyruvate tautomerase PptA, 4-oxalocrotonate tautomerase family [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 441545  Cd Length: 58  Bit Score: 70.18  E-value: 2.01e-18
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*.
gi 489074719  2 PFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFINDMPEGTYYPQGEM 57
Cdd:COG1942   1 PIINIKLFEGRTPEQKRALAKAVTDAVVEVLGKPPESVSVVIEEVPPENWGIGGKL 56
4Oxalocrotonate_Tautomerase cd00491
4-Oxalocrotonate Tautomerase: Catalyzes the isomerization of unsaturated ketones. The ...
 2-56 1.36e-15

4-Oxalocrotonate Tautomerase: Catalyzes the isomerization of unsaturated ketones. The structure is a homohexamer that is arranged as a trimer of dimers. The hexamer contains six active sites, each formed by residues from three monomers, two from one dimer and the third from a neighboring monomer. Each monomer is a beta-alpha-beta fold with two small beta strands at the C-terminus that fold back on themselves. A pair of monomers form a dimer with two-fold symmetry, consisting of a 4-stranded beta sheet with two helices on one side and two additional small beta strands at each end. The dimers are assembled around a 3-fold axis of rotation to form a hexamer, with the short beta strands from each dimer contacting the neighboring dimers.


Pssm-ID: 238274  Cd Length: 58  Bit Score: 63.18  E-value: 1.36e-15
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*
gi 489074719  2 PFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFINDMPEGTYYPQGE 56
Cdd:cd00491   1 PFVQIYILEGRTDEQKRELIERVTEAVSEILGAPEATIVVIIDEMPKENWGIGGE 55
taut TIGR00013
4-oxalocrotonate tautomerase family enzyme; 4-oxalocrotonate tautomerase is a homohexamer in ...
 2-56 1.11e-09

4-oxalocrotonate tautomerase family enzyme; 4-oxalocrotonate tautomerase is a homohexamer in which each monomer is very small, at about 62 amino acids. Pro-1 of the mature protein serves as a general base. The enzyme functions in meta-cleavage pathways of aromatic hydrocarbon catabolism. Because several Arg residues located near the active site in the crystal structure of Pseudomonas putida are not conserved among all members of this family, because the literature describes a general role in the isomerization of beta,gamma-unsaturated enones to their alpha,beta-isomers, and because of the presence of fairly distantly related paralogs in Campylobacter jejuni, the family is regarded as not necessarily uniform in function. [Energy metabolism, Other]


Pssm-ID: 129125  Cd Length: 63  Bit Score: 48.19  E-value: 1.11e-09
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 489074719   2 PFVTID-LFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFINDMPEGTYYPQGE 56
Cdd:TIGR00013  1 PFVNIYiLKEGRTDEQKRQLIEGVTEAMAETLGANLESIVVIIDEMPKNNYGIGGE 56
 
Name Accession Description Interval E-value
PRK02289 PRK02289
4-oxalocrotonate tautomerase; Provisional
 1-60 1.22e-37

4-oxalocrotonate tautomerase; Provisional


Pssm-ID: 179406  Cd Length: 60  Bit Score: 118.88  E-value: 1.22e-37
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 489074719  1 MPFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFINDMPEGTYYPQGEMKQK 60
Cdd:PRK02289  1 MPFVRIDLFEGRSQEQKNALAREVTEVVSRIAKAPKEAIHVFINDMPEGTYYPQGEMKTK 60
Tautomerase pfam01361
Tautomerase enzyme; This family includes the enzyme 4-oxalocrotonate tautomerase that ...
 2-58 9.07e-23

Tautomerase enzyme; This family includes the enzyme 4-oxalocrotonate tautomerase that catalyzes the ketonization of 2-hydroxymuconate to 2-oxo-3-hexenedioate.


Pssm-ID: 396090  Cd Length: 60  Bit Score: 81.18  E-value: 9.07e-23
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 489074719   2 PFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFINDMPEGTYYPQGEMK 58
Cdd:pfam01361  1 PFVNIKLFEGRTDEQKKELIERVTDALVEVLGAPRSAIVVIIEEVPPENWGVGGESV 57
PRK02220 PRK02220
4-oxalocrotonate tautomerase; Provisional
 1-58 5.95e-20

4-oxalocrotonate tautomerase; Provisional


Pssm-ID: 235013  Cd Length: 61  Bit Score: 74.41  E-value: 5.95e-20
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 489074719  1 MPFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFINDMPEGTYYPQGEMK 58
Cdd:PRK02220  1 MPYVHIKLIEGRTEEQLKALVKDVTAAVSKNTGAPAEHIHVIINEMSKNHYAVGGKRL 58
PptA COG1942
Phenylpyruvate tautomerase PptA, 4-oxalocrotonate tautomerase family [Secondary metabolites ...
 2-57 2.01e-18

Phenylpyruvate tautomerase PptA, 4-oxalocrotonate tautomerase family [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 441545  Cd Length: 58  Bit Score: 70.18  E-value: 2.01e-18
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*.
gi 489074719  2 PFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFINDMPEGTYYPQGEM 57
Cdd:COG1942   1 PIINIKLFEGRTPEQKRALAKAVTDAVVEVLGKPPESVSVVIEEVPPENWGIGGKL 56
4Oxalocrotonate_Tautomerase cd00491
4-Oxalocrotonate Tautomerase: Catalyzes the isomerization of unsaturated ketones. The ...
 2-56 1.36e-15

4-Oxalocrotonate Tautomerase: Catalyzes the isomerization of unsaturated ketones. The structure is a homohexamer that is arranged as a trimer of dimers. The hexamer contains six active sites, each formed by residues from three monomers, two from one dimer and the third from a neighboring monomer. Each monomer is a beta-alpha-beta fold with two small beta strands at the C-terminus that fold back on themselves. A pair of monomers form a dimer with two-fold symmetry, consisting of a 4-stranded beta sheet with two helices on one side and two additional small beta strands at each end. The dimers are assembled around a 3-fold axis of rotation to form a hexamer, with the short beta strands from each dimer contacting the neighboring dimers.


Pssm-ID: 238274  Cd Length: 58  Bit Score: 63.18  E-value: 1.36e-15
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*
gi 489074719  2 PFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFINDMPEGTYYPQGE 56
Cdd:cd00491   1 PFVQIYILEGRTDEQKRELIERVTEAVSEILGAPEATIVVIIDEMPKENWGIGGE 55
PRK01964 PRK01964
4-oxalocrotonate tautomerase; Provisional
 1-47 7.29e-11

4-oxalocrotonate tautomerase; Provisional


Pssm-ID: 179355  Cd Length: 64  Bit Score: 51.34  E-value: 7.29e-11
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*..
gi 489074719  1 MPFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFINDMP 47
Cdd:PRK01964  1 MPIVQIQLLEGRPEEKIKNLIREVTEAISATLDVPKERVRVIVNEVP 47
taut TIGR00013
4-oxalocrotonate tautomerase family enzyme; 4-oxalocrotonate tautomerase is a homohexamer in ...
 2-56 1.11e-09

4-oxalocrotonate tautomerase family enzyme; 4-oxalocrotonate tautomerase is a homohexamer in which each monomer is very small, at about 62 amino acids. Pro-1 of the mature protein serves as a general base. The enzyme functions in meta-cleavage pathways of aromatic hydrocarbon catabolism. Because several Arg residues located near the active site in the crystal structure of Pseudomonas putida are not conserved among all members of this family, because the literature describes a general role in the isomerization of beta,gamma-unsaturated enones to their alpha,beta-isomers, and because of the presence of fairly distantly related paralogs in Campylobacter jejuni, the family is regarded as not necessarily uniform in function. [Energy metabolism, Other]


Pssm-ID: 129125  Cd Length: 63  Bit Score: 48.19  E-value: 1.11e-09
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 489074719   2 PFVTID-LFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFINDMPEGTYYPQGE 56
Cdd:TIGR00013  1 PFVNIYiLKEGRTDEQKRQLIEGVTEAMAETLGANLESIVVIIDEMPKNNYGIGGE 56
PRK00745 PRK00745
4-oxalocrotonate tautomerase; Provisional
 1-57 2.72e-09

4-oxalocrotonate tautomerase; Provisional


Pssm-ID: 179107  Cd Length: 62  Bit Score: 47.41  E-value: 2.72e-09
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*..
gi 489074719  1 MPFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFINDMPEGTYYPQGEM 57
Cdd:PRK00745  1 MPTFHIELFEGRTVEQKRKLVEEITRVTVETLGCPPESVDIIITDVKRENWATGGKL 57
HpaF COG3232
5-carboxymethyl-2-hydroxymuconate isomerase [Amino acid transport and metabolism];
4-52 2.49e-05

5-carboxymethyl-2-hydroxymuconate isomerase [Amino acid transport and metabolism];


Pssm-ID: 442464 [Multi-domain]  Cd Length: 121  Bit Score: 38.30  E-value: 2.49e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|..
gi 489074719   4 VTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKEN---IHVFINDMPEGTYY 52
Cdd:COG3232   63 VTLRILAGRSDEQKQALSEALFAALRAHLAPVFARyvaLSVEIREIDRASYA 114
Tautomerase_2 pfam14552
Tautomerase enzyme;
3-44 1.74e-03

Tautomerase enzyme;


Pssm-ID: 434035  Cd Length: 82  Bit Score: 32.92  E-value: 1.74e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*
gi 489074719   3 FVTIDLFEGRSQEQKNQLAREVTEvvsRIAKAPkeNI---HVFIN 44
Cdd:pfam14552 31 IIQITLFAGRSVEQKKALYRALAE---RLAAKL--GIrpeDVFIV 70
CHMI cd00580
5-carboxymethyl-2-hydroxymuconate isomerase (CHMI) is a trimeric enzyme catalyzing the ...
 4-43 2.65e-03

5-carboxymethyl-2-hydroxymuconate isomerase (CHMI) is a trimeric enzyme catalyzing the isomerization of the unsaturated ketone 5-(carboxymethyl)-2-hydroxymuconate to 5-(carboxymethyl)-2-oxo-3-hexene-1,6-dionate. This is one step in the homoprotocatechuate pathway, one of the microbial meta-fission pathways that degrade aromatic carbon sources to citric acid cycle intermediates. Despite the structural similarity of CHMI with 4-oxalocrotonate tautomerase (4-OT) and macrophage migration inhibitory factor (MIF), there is no significant sequence similarity among these protein families, and therefore, they are not combined in one hierarchy.


Pssm-ID: 238324 [Multi-domain]  Cd Length: 113  Bit Score: 32.92  E-value: 2.65e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|
gi 489074719   4 VTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFI 43
Cdd:cd00580   63 VTLRILAGRSEEQKQELSEALLAALRAHLAPVFAKRYLSL 102
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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