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Conserved domains on  [gi|488907062|ref|WP_002818137|]
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GNAT family N-acetyltransferase [Oenococcus oeni]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 10006425)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate; similar to Escherichia coli uncharacterized protein YjdJ

CATH:  3.40.630.30
EC:  2.3.1.-|2.3.-.-
Gene Ontology:  GO:0008080|GO:0016746
PubMed:  10940244|9175471|15581578|27367672|26818562
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
YidJ COG2388
Predicted acetyltransferase, GNAT superfamily [General function prediction only];
4-90 1.87e-17

Predicted acetyltransferase, GNAT superfamily [General function prediction only];


:

Pssm-ID: 441953 [Multi-domain]  Cd Length: 88  Bit Score: 70.18  E-value: 1.87e-17
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488907062  4 YQIKFKED--CYYAENNaGKVISELLYSKikQGKVISINQVRVDPLFRNRGIAGKLLKKAVDDAVKNGQLIKAVCSFSKK 81
Cdd:COG2388   1 MEITHNEEkgRFELEVD-GELAGELTYRL--EGGVIIITHTEVPPALRGQGIASALVEAALDDARERGLKVVPLCPFVAA 77


                ....*....
gi 488907062 82 AFLQNKNYQ 90
Cdd:COG2388  78 YFERHPEYA 86
 
Name Accession Description Interval E-value
YidJ COG2388
Predicted acetyltransferase, GNAT superfamily [General function prediction only];
4-90 1.87e-17

Predicted acetyltransferase, GNAT superfamily [General function prediction only];


Pssm-ID: 441953 [Multi-domain]  Cd Length: 88  Bit Score: 70.18  E-value: 1.87e-17
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488907062  4 YQIKFKED--CYYAENNaGKVISELLYSKikQGKVISINQVRVDPLFRNRGIAGKLLKKAVDDAVKNGQLIKAVCSFSKK 81
Cdd:COG2388   1 MEITHNEEkgRFELEVD-GELAGELTYRL--EGGVIIITHTEVPPALRGQGIASALVEAALDDARERGLKVVPLCPFVAA 77


                ....*....
gi 488907062 82 AFLQNKNYQ 90
Cdd:COG2388  78 YFERHPEYA 86
Acetyltransf_CG pfam14542
GCN5-related N-acetyl-transferase; This family of GCN5-related N-acetyl-transferases bind both ...
 13-90 7.31e-16

GCN5-related N-acetyl-transferase; This family of GCN5-related N-acetyl-transferases bind both CoA and acetyl-CoA. They are characterized by highly conserved glycine, a cysteine residue in the acetyl-CoA binding site near the acetyl group, their small size compared with other GNATs and a lack of of an obvious substrate-binding site. It is proposed that they transfer an acetyl group from acetyl-CoA to one or more unidentified aliphatic amines via an acetyl (cysteine) enzyme intermediate. The substrate might be another macromolecule.


Pssm-ID: 434030 [Multi-domain]  Cd Length: 79  Bit Score: 66.00  E-value: 7.31e-16
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 488907062  13 YYAENNAGKVISELLYSKikQGKVISINQVRVDPLFRNRGIAGKLLKKAVDDAVKNGQLIKAVCSFSKKAFLQNKNYQ 90
Cdd:pfam14542  2 FEIRVDGGAEVAFLTYRR--GDGVLIITHTEVPPALRGQGIASKLVKAALDDAREEGLKIVPLCSYVAAYLEKHPEYA 77
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 12-69 4.75e-05

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 38.03  E-value: 4.75e-05
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*....
gi 488907062 12 CYYAENNaGKVISELLYSKI-KQGKVISINQVRVDPLFRNRGIAGKLLKKAVDDAVKNG 69
Cdd:cd04301   1 FLVAEDD-GEIVGFASLSPDgSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERG 58
 
Name Accession Description Interval E-value
YidJ COG2388
Predicted acetyltransferase, GNAT superfamily [General function prediction only];
4-90 1.87e-17

Predicted acetyltransferase, GNAT superfamily [General function prediction only];


Pssm-ID: 441953 [Multi-domain]  Cd Length: 88  Bit Score: 70.18  E-value: 1.87e-17
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488907062  4 YQIKFKED--CYYAENNaGKVISELLYSKikQGKVISINQVRVDPLFRNRGIAGKLLKKAVDDAVKNGQLIKAVCSFSKK 81
Cdd:COG2388   1 MEITHNEEkgRFELEVD-GELAGELTYRL--EGGVIIITHTEVPPALRGQGIASALVEAALDDARERGLKVVPLCPFVAA 77


                ....*....
gi 488907062 82 AFLQNKNYQ 90
Cdd:COG2388  78 YFERHPEYA 86
Acetyltransf_CG pfam14542
GCN5-related N-acetyl-transferase; This family of GCN5-related N-acetyl-transferases bind both ...
 13-90 7.31e-16

GCN5-related N-acetyl-transferase; This family of GCN5-related N-acetyl-transferases bind both CoA and acetyl-CoA. They are characterized by highly conserved glycine, a cysteine residue in the acetyl-CoA binding site near the acetyl group, their small size compared with other GNATs and a lack of of an obvious substrate-binding site. It is proposed that they transfer an acetyl group from acetyl-CoA to one or more unidentified aliphatic amines via an acetyl (cysteine) enzyme intermediate. The substrate might be another macromolecule.


Pssm-ID: 434030 [Multi-domain]  Cd Length: 79  Bit Score: 66.00  E-value: 7.31e-16
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 488907062  13 YYAENNAGKVISELLYSKikQGKVISINQVRVDPLFRNRGIAGKLLKKAVDDAVKNGQLIKAVCSFSKKAFLQNKNYQ 90
Cdd:pfam14542  2 FEIRVDGGAEVAFLTYRR--GDGVLIITHTEVPPALRGQGIASKLVKAALDDAREEGLKIVPLCSYVAAYLEKHPEYA 77
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 1-69 2.94e-05

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 39.42  E-value: 2.94e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488907062    1 MNEYQIKFKEDCYYAENNaGKVISELLYSKI-KQGKVISINQVRVDPLFRNRGIAGKLLKKAVDDAVKNG 69
Cdd:pfam00583  24 LEDWDEDASEGFFVAEED-GELVGFASLSIIdDEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERG 92
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 12-69 4.75e-05

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 38.03  E-value: 4.75e-05
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*....
gi 488907062 12 CYYAENNaGKVISELLYSKI-KQGKVISINQVRVDPLFRNRGIAGKLLKKAVDDAVKNG 69
Cdd:cd04301   1 FLVAEDD-GEIVGFASLSPDgSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERG 58
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
12-69 2.56e-04

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 37.37  E-value: 2.56e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 488907062  12 CYYAENNaGKVISELLYSKIK---QGKVISINQVRVDPLFRNRGIAGKLLKKAVDDAVKNG 69
Cdd:COG3153   41 SLVAEDD-GEIVGHVALSPVDidgEGPALLLGPLAVDPEYRGQGIGRALMRAALEAARERG 100
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 26-69 2.94e-04

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 36.56  E-value: 2.94e-04
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....
gi 488907062 26 LLYSKIKQGKVISINQVRVDPLFRNRGIAGKLLKKAVDDAVKNG 69
Cdd:COG0456   3 ALLGLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERG 46
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
34-69 4.33e-04

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 36.04  E-value: 4.33e-04
                        10        20        30
                ....*....|....*....|....*....|....*.
gi 488907062 34 GKVISINQVRVDPLFRNRGIAGKLLKKAVDDAVKNG 69
Cdd:COG3393  13 PGVAEISGVYTHPEYRGRGLASALVAALAREALARG 48
Eis COG4552
Predicted acetyltransferase [General function prediction only];
42-72 1.40e-03

Predicted acetyltransferase [General function prediction only];


Pssm-ID: 443616 [Multi-domain]  Cd Length: 393  Bit Score: 36.03  E-value: 1.40e-03
                         10        20        30
                 ....*....|....*....|....*....|.
gi 488907062  42 VRVDPLFRNRGIAGKLLKKAVDDAVKNGQLI 72
Cdd:COG4552   78 VAVAPEHRRRGVARALLREALAELRERGQPL 108
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 9-96 2.82e-03

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 33.58  E-value: 2.82e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488907062   9 KEDCYYAENNaGKVISELLYSKIKQGKVISINQVRVDPLFRNRGIAGKLLKKAVDDAVKNG-QLIKAVCSFSKKAFlqnk 87
Cdd:pfam13508  2 GGRFFVAEDD-GKIVGFAALLPLDDEGALAELRLAVHPEYRGQGIGRALLEAAEAAAKEGGiKLLELETTNRAAAF---- 76


                 ....*....
gi 488907062  88 nYQKYEYKE 96
Cdd:pfam13508 77 -YEKLGFEE 84
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 10-69 4.05e-03

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 34.26  E-value: 4.05e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 488907062  10 EDCYYAENNAGKVISELLYSKIkQGKVISINQVRVDPLFRNRGIAGKLLKKAVDDAVKNG 69
Cdd:COG0454   33 GAEFIAVDDKGEPIGFAGLRRL-DDKVLELKRLYVLPEYRGKGIGKALLEALLEWARERG 91
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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