TraB/GumN family protein similar to Enterococcus faecalis bacteriocin plasmid pPD1 TraB which functions in sex pheromone cPD1 shutdown, perhaps acting as a metalloprotease
TraB/PryY confer plasmid-borne pheromone resistance; TraB/PrgY proteins, identified in gut ...
14-223
2.32e-64
TraB/PryY confer plasmid-borne pheromone resistance; TraB/PrgY proteins, identified in gut bacterium Enterococcus faecalis, are plasmid-borne homologs that are induced by pheromones. Induction rends the host bacterium insensitive to self-induction by its own pheromones, and prevents the transfer of the pheromone-inducible conjugative plasmids to bacteria that already contain it. Based on homology to Tiki activity, it has been proposed that TraB acts as a protease in the inactivation of mating pheromone, cleaving at the amino-terminus. The pheromones are small peptides (7-8 residues) encoded by the bacterial genome, and are specific for particular plasmids, or class of plasmids, which may contain several virulence factors and disseminate rapidly. Plasmid-borne antibiotic resistance and virulence determinants make these elements important contributors to medical problems. Trab/PrygY is a member of a Tiki-like superfamily. Tiki is a membrane-associated metalloprotease (MEROPS family M96) that inhibits Wnt via the cleavage of its amino terminus. Wnt is essential in animal development and homeostasis. In Xenopus, Tiki is critical in head development. In human cells, Tiki inhibits Wnt-signaling. Tiki proteins are also related to erythromycin esterase, gumN plant pathogens, RtxA containing toxins, and Campylobacter Jejuni ChaN heme-binding protein.
Pssm-ID: 350608 Cd Length: 177 Bit Score: 203.19 E-value: 2.32e-64
pheromone shutdown-related protein TraB; traB is a plasmid encoded gene that functions in the ...
9-376
8.12e-64
pheromone shutdown-related protein TraB; traB is a plasmid encoded gene that functions in the shutdown of the peptide sex pheromone cPD1 which is produced by the plasmid free recipient cell prior to conjugative transfer in Enterococcus faecalis. Once the recipient acquires the plasmid, production of cPD1 is shut down. The gene product may play another role in the other species in the family. [Unknown function, General]
Pssm-ID: 129363 Cd Length: 380 Bit Score: 208.55 E-value: 8.12e-64
TraB/PrgY/gumN family; This entry includes Tiki1/2 from humans, TraB/PrgY from the gut flora ...
3-224
1.37e-26
TraB/PrgY/gumN family; This entry includes Tiki1/2 from humans, TraB/PrgY from the gut flora Enterococcusfaecalis and gumN from the plant pathogen Xanthomonas. Tiki1 is homologous to TraB/PrgY. They have a pair of widely spaced GX2H motifs and a conserved glutamate. From the structural study, this group of proteins have been identified as an ancient metalloprotease clan with a common protein architecture (cobbled from the folds of the EreA/ChaN/PMT group) that mediates proteolytic activities. Tiki1 is a membrane-associated protease that inhibits Wnt via the cleavage of its amino terminus, diminishing Wnt's binding to receptors. TraB/PrgY is an inhibitor peptide that may act as a protease to inactivate the mating pheromone.
Pssm-ID: 426534 Cd Length: 262 Bit Score: 106.67 E-value: 1.37e-26
TraB/PryY confer plasmid-borne pheromone resistance; TraB/PrgY proteins, identified in gut ...
14-223
2.32e-64
TraB/PryY confer plasmid-borne pheromone resistance; TraB/PrgY proteins, identified in gut bacterium Enterococcus faecalis, are plasmid-borne homologs that are induced by pheromones. Induction rends the host bacterium insensitive to self-induction by its own pheromones, and prevents the transfer of the pheromone-inducible conjugative plasmids to bacteria that already contain it. Based on homology to Tiki activity, it has been proposed that TraB acts as a protease in the inactivation of mating pheromone, cleaving at the amino-terminus. The pheromones are small peptides (7-8 residues) encoded by the bacterial genome, and are specific for particular plasmids, or class of plasmids, which may contain several virulence factors and disseminate rapidly. Plasmid-borne antibiotic resistance and virulence determinants make these elements important contributors to medical problems. Trab/PrygY is a member of a Tiki-like superfamily. Tiki is a membrane-associated metalloprotease (MEROPS family M96) that inhibits Wnt via the cleavage of its amino terminus. Wnt is essential in animal development and homeostasis. In Xenopus, Tiki is critical in head development. In human cells, Tiki inhibits Wnt-signaling. Tiki proteins are also related to erythromycin esterase, gumN plant pathogens, RtxA containing toxins, and Campylobacter Jejuni ChaN heme-binding protein.
Pssm-ID: 350608 Cd Length: 177 Bit Score: 203.19 E-value: 2.32e-64
pheromone shutdown-related protein TraB; traB is a plasmid encoded gene that functions in the ...
9-376
8.12e-64
pheromone shutdown-related protein TraB; traB is a plasmid encoded gene that functions in the shutdown of the peptide sex pheromone cPD1 which is produced by the plasmid free recipient cell prior to conjugative transfer in Enterococcus faecalis. Once the recipient acquires the plasmid, production of cPD1 is shut down. The gene product may play another role in the other species in the family. [Unknown function, General]
Pssm-ID: 129363 Cd Length: 380 Bit Score: 208.55 E-value: 8.12e-64
TraB/PrgY/gumN family; This entry includes Tiki1/2 from humans, TraB/PrgY from the gut flora ...
3-224
1.37e-26
TraB/PrgY/gumN family; This entry includes Tiki1/2 from humans, TraB/PrgY from the gut flora Enterococcusfaecalis and gumN from the plant pathogen Xanthomonas. Tiki1 is homologous to TraB/PrgY. They have a pair of widely spaced GX2H motifs and a conserved glutamate. From the structural study, this group of proteins have been identified as an ancient metalloprotease clan with a common protein architecture (cobbled from the folds of the EreA/ChaN/PMT group) that mediates proteolytic activities. Tiki1 is a membrane-associated protease that inhibits Wnt via the cleavage of its amino terminus, diminishing Wnt's binding to receptors. TraB/PrgY is an inhibitor peptide that may act as a protease to inactivate the mating pheromone.
Pssm-ID: 426534 Cd Length: 262 Bit Score: 106.67 E-value: 1.37e-26
diverse proteins related to the Tiki and TraB protease domains; The extracellular domain of ...
14-100
2.88e-10
diverse proteins related to the Tiki and TraB protease domains; The extracellular domain of Tiki family proteins shares homology with bacterial TraB/PrgY proteins which are known for their roles in the inhibition of mating pheromones. Tiki and TraB/PrgY proteins share limited sequence identity, but their predicted secondary structures reveal that several catalytic residues are anchored in a similar manner, consistent with a common evolutionary origin. Tiki domains are related to the erythromycin esterase, gumN plant pathogens, RtxA toxins, and Campylobacter Jejuni heme-binding, ChaN-like proteins. Tiki is a membrane-associated metalloprotease (MEROPS family M96) that inhibits Wnt via the cleavage of its amino terminus, diminishing Wnt's binding to receptors. Wnt is essential in animal development and homeostasis. In Xenopus, Tiki is critical in head development. In human cells, Tiki inhibits Wnt-signaling, which is important in embryogenesis, homeostasis, and regeneration. Deregulation of Wnt contributes to birth defects, cancer and various diseases. TraB/PrgY protein has been identified in gut bacterium Enterococcus faecalis, but its function has not been well characterized. Plasmid-borne TraB has been implicated in the regulation of pheromone sensitivity and specificity. Based on homology to Tiki activity, it has been proposed that TraB acts as a metalloprotease in the inactivation of mating pheromone. Pasteurella multicida toxin has structural and sequence similarity to the Tiki/TraB family of proteases. However, unlike related multidomain toxins in this family, they do not exhibit conservation of the typical active site residues.
Pssm-ID: 350612 Cd Length: 127 Bit Score: 57.47 E-value: 2.88e-10
Tiki homology domain antagonizes Wnt function via cleavage of amino-terminal residues; Tiki is ...
170-223
2.13e-04
Tiki homology domain antagonizes Wnt function via cleavage of amino-terminal residues; Tiki is a membrane-associated metalloprotease that inhibits Wnt via the cleavage of its amino terminus, diminishing Wnt's binding to receptors. Wnt is essential in animal development and homeostasis. In xenopus, tiki is critical in head development. In human cells, TIKI inhibits Wnt-signaling, which is important in embryogenesis, homeostasis, and regeneration. Deregulation of WNT contributes to birth defects, cancer and various diseases. TIKI homology domains are part of the TraB family and are related to the Erythromycin esterase, GumN plant pathogens, RtxA toxins, and Campylobacter Jejuni heme-binding, Chan-like proteins. TraB/PrgY are identified in gut bacterium Enterococcus faecalis, but its function has not been well characterized. Plasmid-borne, TraB has been implicated in the regulation of pheromone sensitivity and specificity. Based on homology to TIKI activity, it has been proposed that TraB acts as a metalloprotease in the inactivation of mating pheromone. The TIKI/TraB family has 2 conserved GxxH motifs and conserved glutamate and arginine residues that may be catalytic.
Pssm-ID: 350614 Cd Length: 259 Bit Score: 42.67 E-value: 2.13e-04
Database: CDSEARCH/cdd Low complexity filter: no Composition Based Adjustment: yes E-value threshold: 0.01
References:
Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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