WfgS and WfeV are involved in O-antigen biosynthesis; Escherichia coli WfgS and Shigella dysenteriae WfeV are glycosyltransferase 2 family enzymes involved in O-antigen biosynthesis. GT-2 enzymes have GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 459495030   2 ITACFNSEKTIEDTILSVLHQTYKNIEYIIIDGASADSTLGIIQKYKDQIACVVSEKDKGIYDAMNKGIKRASGDIIALL 81
Cdd:cd06433    3 ITPTYNQAETLEETIDSVLSQTYPNIEYIVIDGGSTDGTVDIIKKYEDKITYWISEPDKGIYDAMNKGIALATGDIIGFL 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 459495030  82 NSDDFYIDEFVIEKVVHEFEKKHCDSVYADLVFVKPNRLEKVVRYYESgeFSPKTLLYGVVPAHPTLFVKKAIHERYGLY 161
Cdd:cd06433   83 NSDDTLLPGALLAVVAAFAEHPEVDVVYGDVLLVDENGRVIGRRRPPP--FLDKFLLYGMPICHQATFFRRSLFEKYGGF 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|..
gi 459495030 162 KTDYKISADFEMIIRLFVVQKIsFSYLKEVLVVMRTGGVSAS 203
Cdd:cd06433  161 DESYRIAADYDLLLRLLLAGKI-FKYLPEVLAAFRLGGVSST 201

WfgS and WfeV are involved in O-antigen biosynthesis; Escherichia coli WfgS and Shigella dysenteriae WfeV are glycosyltransferase 2 family enzymes involved in O-antigen biosynthesis. GT-2 enzymes have GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 459495030   2 ITACFNSEKTIEDTILSVLHQTYKNIEYIIIDGASADSTLGIIQKYKDQIACVVSEKDKGIYDAMNKGIKRASGDIIALL 81
Cdd:cd06433    3 ITPTYNQAETLEETIDSVLSQTYPNIEYIVIDGGSTDGTVDIIKKYEDKITYWISEPDKGIYDAMNKGIALATGDIIGFL 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 459495030  82 NSDDFYIDEFVIEKVVHEFEKKHCDSVYADLVFVKPNRLEKVVRYYESgeFSPKTLLYGVVPAHPTLFVKKAIHERYGLY 161
Cdd:cd06433   83 NSDDTLLPGALLAVVAAFAEHPEVDVVYGDVLLVDENGRVIGRRRPPP--FLDKFLLYGMPICHQATFFRRSLFEKYGGF 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|..
gi 459495030 162 KTDYKISADFEMIIRLFVVQKIsFSYLKEVLVVMRTGGVSAS 203
Cdd:cd06433  161 DESYRIAADYDLLLRLLLAGKI-FKYLPEVLAAFRLGGVSST 201

Glycosyl transferase family 2; Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 459495030    2 ITACFNSEKTIEDTILSVLHQTYKNIEYIIIDGASADSTLGIIQKYKDQ---IACVVSEKDKGIYDAMNKGIKRASGDII 78
Cdd:pfam00535   3 IIPTYNEEKYLLETLESLLNQTYPNFEIIVVDDGSTDGTVEIAEEYAKKdprVRVIRLPENRGKAGARNAGLRAATGDYI 82

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 459495030   79 ALLNSDDFYIDEFvIEKVVHEFEKKHCDSVYADLVFVKPNRLEKVVRYYESGEFSPKTLLYGVVP 143
Cdd:pfam00535  83 AFLDADDEVPPDW-LEKLVEALEEDGADVVVGSRYVIFGETGEYRRASRITLSRLPFFLGLRLLG 146

WfgS and WfeV are involved in O-antigen biosynthesis; Escherichia coli WfgS and Shigella dysenteriae WfeV are glycosyltransferase 2 family enzymes involved in O-antigen biosynthesis. GT-2 enzymes have GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 459495030   2 ITACFNSEKTIEDTILSVLHQTYKNIEYIIIDGASADSTLGIIQKYKDQIACVVSEKDKGIYDAMNKGIKRASGDIIALL 81
Cdd:cd06433    3 ITPTYNQAETLEETIDSVLSQTYPNIEYIVIDGGSTDGTVDIIKKYEDKITYWISEPDKGIYDAMNKGIALATGDIIGFL 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 459495030  82 NSDDFYIDEFVIEKVVHEFEKKHCDSVYADLVFVKPNRLEKVVRYYESgeFSPKTLLYGVVPAHPTLFVKKAIHERYGLY 161
Cdd:cd06433   83 NSDDTLLPGALLAVVAAFAEHPEVDVVYGDVLLVDENGRVIGRRRPPP--FLDKFLLYGMPICHQATFFRRSLFEKYGGF 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|..
gi 459495030 162 KTDYKISADFEMIIRLFVVQKIsFSYLKEVLVVMRTGGVSAS 203
Cdd:cd06433  161 DESYRIAADYDLLLRLLLAGKI-FKYLPEVLAAFRLGGVSST 201

Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold; Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. Based on the stereochemistry of the donor and acceptor molecules, GTs are classified as either retaining or inverting enzymes. To date, all GT structures adopt one of two possible folds, termed GT-A fold and GT-B fold. This hierarchy includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. But it also includes families GT-43, GT-6, GT-8, GT13 and GT-7; which are evolutionarily related to GT-2 and share structure similarities.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 459495030   2 ITACFNSEKTIEDTILSVLHQTYKNIEYIIIDGASADSTLGIIQKYKDQ---IACVVSEKDKGIYDAMNKGIKRASGDII 78
Cdd:cd00761    2 IIPAYNEEPYLERCLESLLAQTYPNFEVIVVDDGSTDGTLEILEEYAKKdprVIRVINEENQGLAAARNAGLKAARGEYI 81

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 459495030  79 ALLNSDDFYIDEFVIEKVVHEFEKKHCDSVYAD-LVFVKPNRLEKVVRYYESGEF 132
Cdd:cd00761   82 LFLDADDLLLPDWLERLVAELLADPEADAVGGPgNLLFRRELLEEIGGFDEALLS 136

ExoA is involved in the biosynthesis of succinoglycan; Succinoglycan Biosynthesis Protein ExoA catalyzes the formation of a beta-1,3 linkage of the second sugar (glucose) of the succinoglycan with the galactose on the lipid carrie. Succinoglycan is an acidic exopolysaccharide that is important for invasion of the nodules. Succinoglycan is a high-molecular-weight polymer composed of repeating octasaccharide units. These units are synthesized on membrane-bound isoprenoid lipid carriers, beginning with galactose followed by seven glucose molecules, and modified by the addition of acetate, succinate, and pyruvate. ExoA is a membrane protein with a transmembrance domain at c-terminus.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 459495030   2 ITACFNSEKTIEDTILSVLHQTY--KNIEYIIIDGASADSTLGIIQKY--KDQIACVVSEKDKGIYDAMNKGIKRASGDI 77
Cdd:cd02525    5 IIPVRNEEKYIEELLESLLNQSYpkDLIEIIVVDGGSTDGTREIVQEYaaKDPRIRLIDNPKRIQSAGLNIGIRNSRGDI 84

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 459495030  78 IALLNSDDFYIDEFvIEKVVHEFEKKHCDSVYADLVFVKPNRLEKVVRYYESGEFSPKTLLY-------GVVPAHPTLFV 150
Cdd:cd02525   85 IIRVDAHAVYPKDY-ILELVEALKRTGADNVGGPMETIGESKFQKAIAVAQSSPLGSGGSAYrggavkiGYVDTVHHGAY 163

                        170       180
                 ....*....|....*....|....*..
gi 459495030 151 KKAIHERYGLYKTDYKISADFEMIIRL 177
Cdd:cd02525  164 RREVFEKVGGFDESLVRNEDAELNYRL 190

Glycosyltransferase, GT2 family [Carbohydrate transport and metabolism];

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 459495030   2 ITACFNSEKTIEDTILSVLHQTYKNIEYIIIDGASADSTLGIIQKYK-DQIACVVSEKDKGIYDAMNKGIKRASGDIIAL 80
Cdd:COG1216    8 VIPTYNRPELLRRCLESLLAQTYPPFEVIVVDNGSTDGTAELLAALAfPRVRVIRNPENLGFAAARNLGLRAAGGDYLLF 87

                         90
                 ....*....|..
gi 459495030  81 LNSDDFYIDEFV 92
Cdd:COG1216   88 LDDDTVVEPDWL 99

CESA_like is the cellulose synthase superfamily; The cellulose synthase (CESA) superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins. Cellulose synthase catalyzes the polymerization reaction of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues in plants, most algae, some bacteria and fungi, and even some animals. In bacteria, algae and lower eukaryotes, there is a second unrelated type of cellulose synthase (Type II), which produces acylated cellulose, a derivative of cellulose. Chitin synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of beta-(1,4)-linked GlcNAc residues and Glucan Biosynthesis protein catalyzes the elongation of beta-1,2 polyglucose chains of Glucan.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 459495030   2 ITACFNSEKTIEDTILSVLHQTYKNIEYIIIDGASADSTLGIIQKYKDQIAC----VVSEKDKGIYDAMNKGIKRASGDI 77
Cdd:cd06423    2 IVPAYNEEAVIERTIESLLALDYPKLEVIVVDDGSTDDTLEILEELAALYIRrvlvVRDKENGGKAGALNAGLRHAKGDI 81

                         90       100
                 ....*....|....*....|....*.
gi 459495030  78 IALLNSdDFYIDEFVIEKVVHEFEKK 103
Cdd:cd06423   82 VVVLDA-DTILEPDALKRLVVPFFAD 106

DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily; DPM1 is the catalytic subunit of eukaryotic dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. In higher eukaryotes,the enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. In lower eukaryotes, such as Saccharomyces cerevisiae and Trypanosoma brucei, DPM synthase consists of a single component (Dpm1p and TbDpm1, respectively) that possesses one predicted transmembrane region near the C terminus for anchoring to the ER membrane. In contrast, the Dpm1 homologues of higher eukaryotes, namely fission yeast, fungi, and animals, have no transmembrane region, suggesting the existence of adapter molecules for membrane anchoring. This family also includes bacteria and archaea DPM1_like enzymes. However, the enzyme structure and mechanism of function are not well understood. The UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate. This protein family belongs to Glycosyltransferase 2 superfamily.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 459495030   2 ITACFNSEKTIEDT---ILSVLHQTYkNIEYIIIDGASADSTLGIIQKYKDQ---IACVVSEKDKGIYDAMNKGIKRASG 75
Cdd:cd04179    2 VIPAYNEEENIPELverLLAVLEEGY-DYEIIVVDDGSTDGTAEIARELAARvprVRVIRLSRNFGKGAAVRAGFKAARG 80

                         90       100       110
                 ....*....|....*....|....*....|....*
gi 459495030  76 DIIALLNSDDFYiDEFVIEKVVHEFEKKHCDSVYA 110
Cdd:cd04179   81 DIVVTMDADLQH-PPEDIPKLLEKLLEGGADVVIG 114

CESA_like_1 is a member of the cellulose synthase (CESA) superfamily; This is a subfamily of cellulose synthase (CESA) superfamily. CESA superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members of the superfamily include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 459495030   1 MITACFNSEKTIEDTILSVLHQTYKN--IEYIIIDGASADSTLGIIQKYKDQIACVVSEKD-KGIYDAMNKGIKRASGDI 77
Cdd:cd06439   33 IIIPAYNEEAVIEAKLENLLALDYPRdrLEIIVVSDGSTDGTAEIAREYADKGVKLLRFPErRGKAAALNRALALATGEI 112

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 459495030  78 IALlnSD-DFYIDEFVIEKVVHEFEKKHCDSVYADLVFVKPN----------RLEKVVRYYESgefspktLLYGVVPAHP 146
Cdd:cd06439  113 VVF--TDaNALLDPDALRLLVRHFADPSVGAVSGELVIVDGGgsgsgeglywKYENWLKRAES-------RLGSTVGANG 183

                        170
                 ....*....|.
gi 459495030 147 TLF-VKKAIHE 156
Cdd:cd06439  184 AIYaIRRELFR 194

putative glycosyl transferase; Provisional

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 459495030   2 ITACFNSEKTIEDTILSVLHQTYKNIEYIIIDGASADSTLGIIQKYKDQIA--CVVSEKDKGIYDAMNKGIKRASGDIIA 79
Cdd:PRK10073  11 IIPLYNAGKDFRAFMESLIAQTWTALEIIIVNDGSTDNSVEIAKHYAENYPhvRLLHQANAGVSVARNTGLAVATGKYVA 90

                 ....*.
gi 459495030  80 LLNSDD 85
Cdd:PRK10073  91 FPDADD 96

Beta 1, 3-N-acetylglucosaminyltransferase is essential for the formation of poly-N-acetyllactosamine ; This family includes human Beta3GnTL1 and related eukaryotic proteins. Human Beta3GnTL1 is a putative beta-1,3-N-acetylglucosaminyltransferase. Beta3GnTL1 is expressed at various levels in most of tissues examined. Beta 1, 3-N-acetylglucosaminyltransferase has been found to be essential for the formation of poly-N-acetyllactosamine. Poly-N-acetyllactosamine is a unique carbohydrate composed of N-acetyllactosamine repeats. It is often an important part of cell-type-specific oligosaccharide structures and some functional oligosaccharides. It has been shown that the structure and biosynthesis of poly-N-acetyllactosamine display a dramatic change during development and oncogenesis. Several members of beta-1, 3-N-acetylglucosaminyltransferase have been identified.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 459495030   1 MITACFNSEKTIEDTILSVLHQTYKN-IEYIIIDGASADSTLGIIQKYKDQ-----IACVVSEKD----KGIYDAMNKGI 70
Cdd:cd06913    1 IILPVHNGEQWLDECLESVLQQDFEGtLELSVFNDASTDKSAEIIEKWRKKledsgVIVLVGSHNspspKGVGYAKNQAI 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 459495030  71 KRASGDIIALLNSDDFYIDEFVieKVVHEFEKKHCDSVYADLVF-VKPNRLEKVVRYYESgeFSPKTLLYGVVPAH---- 145
Cdd:cd06913   81 AQSSGRYLCFLDSDDVMMPQRI--RLQYEAALQHPNSIIGCQVRrIPEDSTERYTRWINT--LTREQLLTQVYTSHgptv 156

                        170
                 ....*....|....*...
gi 459495030 146 --PTLFVKKAIHERYGLY 161
Cdd:cd06913  157 imPTWFCSREWFSHVGPF 174

Glycosyltransferase like family 2; Members of this family of prokaryotic proteins include putative glucosyltransferase, which are involved in bacterial capsule biosynthesis.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 459495030    2 ITACFNSEKTIEDTILSVLHQTYKNIEYIIIDGASADSTL----GIIQKYKDQ-IACVVSEKDKGIY---DAMNKGIKRA 73
Cdd:pfam13641   7 VVPAFNEDSVLGRVLEAILAQPYPPVEVVVVVNPSDAETLdvaeEIAARFPDVrLRVIRNARLLGPTgksRGLNHGFRAV 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 459495030   74 SGDIIALLNSDDFyIDEFVIEKVVHEFEKKHCDSVYADlVFVK--------PNRLEKVVRYyesGEFSPKTLLYGVVPAH 145
Cdd:pfam13641  87 KSDLVVLHDDDSV-LHPGTLKKYVQYFDSPKVGAVGTP-VFSLnrstmlsaLGALEFALRH---LRMMSLRLALGVLPLS 161

                  ....*
gi 459495030  146 PTLFV 150
Cdd:pfam13641 162 GAGSA 166

N-terminal domain of Chondroitin polymerase functions as a GalNAc transferase; Chondroitin polymerase is a two domain, bi-functional protein. The N-terminal domain functions as a GalNAc transferase. The bacterial chondroitin polymerase catalyzes elongation of the chondroitin chain by alternatively transferring the GlcUA and GalNAc moiety from UDP-GlcUA and UDP-GalNAc to the non-reducing ends of the chondroitin chain. The enzyme consists of N-terminal and C-terminal domains in which the two active sites catalyze the addition of GalNAc and GlcUA, respectively. Chondroitin chains range from 40 to over 100 repeating units of the disaccharide. Sulfated chondroitins are involved in the regulation of various biological functions such as central nervous system development, wound repair, infection, growth factor signaling, and morphogenesis, in addition to its conventional structural roles. In Caenorhabditis elegans, chondroitin is an essential factor for the worm to undergo cytokinesis and cell division. Chondroitin is synthesized as proteoglycans, sulfated and secreted to the cell surface or extracellular matrix.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 459495030   2 ITACFNSEKTIEDTILSVLHQTYKNIEYIIIDGASADSTLGIIQKYKDQIACVVS---EKDKGIYDA--MNKGIKRASGD 76
Cdd:cd06420    2 IITTYNRPEALELVLKSVLNQSILPFEVIIADDGSTEETKELIEEFKSQFPIPIKhvwQEDEGFRKAkiRNKAIAAAKGD 81

                 ....*...
gi 459495030  77 IIALLNSD 84
Cdd:cd06420   82 YLIFIDGD 89

DPM1_like represents putative enzymes similar to eukaryotic DPM1; Proteins similar to eukaryotic DPM1, including enzymes from bacteria and archaea; DPM1 is the catalytic subunit of eukaryotic dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. In higher eukaryotes,the enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. In lower eukaryotes, such as Saccharomyces cerevisiae and Trypanosoma brucei, DPM synthase consists of a single component (Dpm1p and TbDpm1, respectively) that possesses one predicted transmembrane region near the C terminus for anchoring to the ER membrane. In contrast, the Dpm1 homologues of higher eukaryotes, namely fission yeast, fungi, and animals, have no transmembrane region, suggesting the existence of adapter molecules for membrane anchoring. This family also includes bacteria and archaea DPM1_like enzymes. However, the enzyme structure and mechanism of function are not well understood. This protein family belongs to Glycosyltransferase 2 superfamily.

                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 459495030  16 ILSVLHQTYKNIEY--IIIDGASADSTLGIIQKYKDQ---IACVVSEKDKGIYDAMNKGIKRASGDIIALLNSD 84
Cdd:cd06442   15 LIERLDAALKGIDYeiIVVDDNSPDGTAEIVRELAKEyprVRLIVRPGKRGLGSAYIEGFKAARGDVIVVMDAD 88

pp-GalNAc-T initiates the formation of mucin-type O-linked glycans; UDP-GalNAc: polypeptide alpha-N-acetylgalactosaminyltransferases (pp-GalNAc-T) initiate the formation of mucin-type, O-linked glycans by catalyzing the transfer of alpha-N-acetylgalactosamine (GalNAc) from UDP-GalNAc to hydroxyl groups of Ser or Thr residues of core proteins to form the Tn antigen (GalNAc-a-1-O-Ser/Thr). These enzymes are type II membrane proteins with a GT-A type catalytic domain and a lectin domain located on the lumen side of the Golgi apparatus. In human, there are 15 isozymes of pp-GalNAc-Ts, representing the largest of all glycosyltransferase families. Each isozyme has unique but partially redundant substrate specificity for glycosylation sites on acceptor proteins.

                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 459495030  15 TILSVLHQTYKN--IEYIIIDGASADSTLGI-----IQKYKDQIACVVSEKDKGIYDAMNKGIKRASGDIIALLNS 83
Cdd:cd02510   17 TVHSVINRTPPEllKEIILVDDFSDKPELKLlleeyYKKYLPKVKVLRLKKREGLIRARIAGARAATGDVLVFLDS 92