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Conserved domains on  [gi|45597404|ref|NP_996689|]
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RusA-like Holliday junction resolvase [Lactococcus phage phiLC3]

Protein Classification

RusA family crossover junction endodeoxyribonuclease( domain architecture ID 10531192)

RusA family crossover junction endodeoxyribonuclease similar to crossover junction endodeoxyribonuclease RusA that resolves Holliday junction intermediates and promotes DNA repair

CATH:  3.30.1330.70
EC:  3.1.21.10
Gene Ontology:  GO:0006281|GO:0006310|GO:0000287|GO:0000400|GO:0008821
PubMed:  17028102|10482492

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RusA pfam05866
Endodeoxyribonuclease RusA; This family consists of several bacterial and phage Holliday ...
 11-127 4.09e-24

Endodeoxyribonuclease RusA; This family consists of several bacterial and phage Holliday junction resolvase (RusA) like proteins. The RusA protein of Escherichia coli is an endonuclease that can resolve Holliday intermediates and correct the defects in genetic recombination and DNA repair associated with inactivation of RuvAB or RuvC.


:

Pssm-ID: 428654  Cd Length: 124  Bit Score: 89.65  E-value: 4.09e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 45597404    11 PTTQQQKGIKKVKGklQFYDRRGTNNYSLK-AQLMKNKPKECFEKNVPLKLSVTFFYAI-------KQKKRWWQWKTSRP 82
Cdd:pfam05866   3 PVGKGRPRFRRVGG--RTYTPEKTKAYENLvKLYLAQQMPGRPPLEGPVKVEIKAYFPIpkswskkKRALEGKIRPTKKP 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 45597404    83 DLDNLMKNLQDYMTKLrYYSDDSQIVWLEAKKVNDEKNRIEIEIT 127
Cdd:pfam05866  81 DIDNLAKALLDALNGG-VWKDDSQVVDIIVEKFYSEEPRVEVEIE 124
 
Name Accession Description Interval E-value
RusA pfam05866
Endodeoxyribonuclease RusA; This family consists of several bacterial and phage Holliday ...
 11-127 4.09e-24

Endodeoxyribonuclease RusA; This family consists of several bacterial and phage Holliday junction resolvase (RusA) like proteins. The RusA protein of Escherichia coli is an endonuclease that can resolve Holliday intermediates and correct the defects in genetic recombination and DNA repair associated with inactivation of RuvAB or RuvC.


Pssm-ID: 428654  Cd Length: 124  Bit Score: 89.65  E-value: 4.09e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 45597404    11 PTTQQQKGIKKVKGklQFYDRRGTNNYSLK-AQLMKNKPKECFEKNVPLKLSVTFFYAI-------KQKKRWWQWKTSRP 82
Cdd:pfam05866   3 PVGKGRPRFRRVGG--RTYTPEKTKAYENLvKLYLAQQMPGRPPLEGPVKVEIKAYFPIpkswskkKRALEGKIRPTKKP 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 45597404    83 DLDNLMKNLQDYMTKLrYYSDDSQIVWLEAKKVNDEKNRIEIEIT 127
Cdd:pfam05866  81 DIDNLAKALLDALNGG-VWKDDSQVVDIIVEKFYSEEPRVEVEIE 124
RusA COG4570
Holliday junction resolvase RusA (prophage-encoded endonuclease) [Replication, recombination ...
 1-129 2.64e-23

Holliday junction resolvase RusA (prophage-encoded endonuclease) [Replication, recombination and repair];


Pssm-ID: 443627  Cd Length: 120  Bit Score: 87.25  E-value: 2.64e-23
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 45597404   1 MKFEFELDKMPTTQQQKgiKKVKGKlqFYDRRGTNNYS--LKAQLMKNKPKECFEKnvPLKLSVTFFYaikqkkrwwQWK 78
Cdd:COG4570   1 MMIKFTLPGPPSPKGRP--RTKRGR--RYTPEKGKAYRnlVALAARQQMPKKPLEG--PVAVEIKFYF---------PPD 65

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|..
gi 45597404  79 TSRPDLDNLMKNLQDYMTKLRYYSDDSQIVWLEAKKVNDEKN-RIEIEITEV 129
Cdd:COG4570  66 RRKPDLDNLLKAILDALTGAGVWKDDSQVVDLIVEKRYDDKGpRVEVEIEEL 117
 
Name Accession Description Interval E-value
RusA pfam05866
Endodeoxyribonuclease RusA; This family consists of several bacterial and phage Holliday ...
 11-127 4.09e-24

Endodeoxyribonuclease RusA; This family consists of several bacterial and phage Holliday junction resolvase (RusA) like proteins. The RusA protein of Escherichia coli is an endonuclease that can resolve Holliday intermediates and correct the defects in genetic recombination and DNA repair associated with inactivation of RuvAB or RuvC.


Pssm-ID: 428654  Cd Length: 124  Bit Score: 89.65  E-value: 4.09e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 45597404    11 PTTQQQKGIKKVKGklQFYDRRGTNNYSLK-AQLMKNKPKECFEKNVPLKLSVTFFYAI-------KQKKRWWQWKTSRP 82
Cdd:pfam05866   3 PVGKGRPRFRRVGG--RTYTPEKTKAYENLvKLYLAQQMPGRPPLEGPVKVEIKAYFPIpkswskkKRALEGKIRPTKKP 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 45597404    83 DLDNLMKNLQDYMTKLrYYSDDSQIVWLEAKKVNDEKNRIEIEIT 127
Cdd:pfam05866  81 DIDNLAKALLDALNGG-VWKDDSQVVDIIVEKFYSEEPRVEVEIE 124
RusA COG4570
Holliday junction resolvase RusA (prophage-encoded endonuclease) [Replication, recombination ...
 1-129 2.64e-23

Holliday junction resolvase RusA (prophage-encoded endonuclease) [Replication, recombination and repair];


Pssm-ID: 443627  Cd Length: 120  Bit Score: 87.25  E-value: 2.64e-23
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 45597404   1 MKFEFELDKMPTTQQQKgiKKVKGKlqFYDRRGTNNYS--LKAQLMKNKPKECFEKnvPLKLSVTFFYaikqkkrwwQWK 78
Cdd:COG4570   1 MMIKFTLPGPPSPKGRP--RTKRGR--RYTPEKGKAYRnlVALAARQQMPKKPLEG--PVAVEIKFYF---------PPD 65

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|..
gi 45597404  79 TSRPDLDNLMKNLQDYMTKLRYYSDDSQIVWLEAKKVNDEKN-RIEIEITEV 129
Cdd:COG4570  66 RRKPDLDNLLKAILDALTGAGVWKDDSQVVDLIVEKRYDDKGpRVEVEIEEL 117
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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