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Conserved domains on  [gi|447069915|ref|WP_001147171|]
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MULTISPECIES: 2-hydroxymuconate tautomerase [Bacillus]

Protein Classification

4-oxalocrotonate tautomerase family protein( domain architecture ID 10011761)

4-oxalocrotonate tautomerase family protein similar to 4-oxalocrotonate tautomerase, also called 2-hydroxymuconate tautomerase, which catalyzes the ketonization of 2-hydroxymuconate stereoselectively to yield 2-oxo-3-hexenedioate

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK02220 PRK02220
4-oxalocrotonate tautomerase; Provisional
 1-61 1.77e-29

4-oxalocrotonate tautomerase; Provisional


:

Pssm-ID: 235013  Cd Length: 61  Bit Score: 98.30  E-value: 1.77e-29
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 447069915  1 MPYVTVKMLEGRTEEQKKALAEKVTAAVSETTGAPEENIVVFIEEMSKNHYAVGGKRLSDK 61
Cdd:PRK02220  1 MPYVHIKLIEGRTEEQLKALVKDVTAAVSKNTGAPAEHIHVIINEMSKNHYAVGGKRLSDK 61
 
Name Accession Description Interval E-value
PRK02220 PRK02220
4-oxalocrotonate tautomerase; Provisional
 1-61 1.77e-29

4-oxalocrotonate tautomerase; Provisional


Pssm-ID: 235013  Cd Length: 61  Bit Score: 98.30  E-value: 1.77e-29
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 447069915  1 MPYVTVKMLEGRTEEQKKALAEKVTAAVSETTGAPEENIVVFIEEMSKNHYAVGGKRLSDK 61
Cdd:PRK02220  1 MPYVHIKLIEGRTEEQLKALVKDVTAAVSKNTGAPAEHIHVIINEMSKNHYAVGGKRLSDK 61
4Oxalocrotonate_Tautomerase cd00491
4-Oxalocrotonate Tautomerase: Catalyzes the isomerization of unsaturated ketones. The ...
 2-59 1.45e-23

4-Oxalocrotonate Tautomerase: Catalyzes the isomerization of unsaturated ketones. The structure is a homohexamer that is arranged as a trimer of dimers. The hexamer contains six active sites, each formed by residues from three monomers, two from one dimer and the third from a neighboring monomer. Each monomer is a beta-alpha-beta fold with two small beta strands at the C-terminus that fold back on themselves. A pair of monomers form a dimer with two-fold symmetry, consisting of a 4-stranded beta sheet with two helices on one side and two additional small beta strands at each end. The dimers are assembled around a 3-fold axis of rotation to form a hexamer, with the short beta strands from each dimer contacting the neighboring dimers.


Pssm-ID: 238274  Cd Length: 58  Bit Score: 83.21  E-value: 1.45e-23
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 447069915  2 PYVTVKMLEGRTEEQKKALAEKVTAAVSETTGAPEENIVVFIEEMSKNHYAVGGKRLS 59
Cdd:cd00491   1 PFVQIYILEGRTDEQKRELIERVTEAVSEILGAPEATIVVIIDEMPKENWGIGGESAS 58
PptA COG1942
Phenylpyruvate tautomerase PptA, 4-oxalocrotonate tautomerase family [Secondary metabolites ...
 2-59 2.60e-23

Phenylpyruvate tautomerase PptA, 4-oxalocrotonate tautomerase family [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 441545  Cd Length: 58  Bit Score: 82.50  E-value: 2.60e-23
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 447069915  2 PYVTVKMLEGRTEEQKKALAEKVTAAVSETTGAPEENIVVFIEEMSKNHYAVGGKRLS 59
Cdd:COG1942   1 PIINIKLFEGRTPEQKRALAKAVTDAVVEVLGKPPESVSVVIEEVPPENWGIGGKLLS 58
Tautomerase pfam01361
Tautomerase enzyme; This family includes the enzyme 4-oxalocrotonate tautomerase that ...
 2-61 2.63e-22

Tautomerase enzyme; This family includes the enzyme 4-oxalocrotonate tautomerase that catalyzes the ketonization of 2-hydroxymuconate to 2-oxo-3-hexenedioate.


Pssm-ID: 396090  Cd Length: 60  Bit Score: 80.03  E-value: 2.63e-22
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 447069915   2 PYVTVKMLEGRTEEQKKALAEKVTAAVSETTGAPEENIVVFIEEMSKNHYAVGGKRLSDK 61
Cdd:pfam01361  1 PFVNIKLFEGRTDEQKKELIERVTDALVEVLGAPRSAIVVIIEEVPPENWGVGGESVGDR 60
taut TIGR00013
4-oxalocrotonate tautomerase family enzyme; 4-oxalocrotonate tautomerase is a homohexamer in ...
 2-60 1.07e-19

4-oxalocrotonate tautomerase family enzyme; 4-oxalocrotonate tautomerase is a homohexamer in which each monomer is very small, at about 62 amino acids. Pro-1 of the mature protein serves as a general base. The enzyme functions in meta-cleavage pathways of aromatic hydrocarbon catabolism. Because several Arg residues located near the active site in the crystal structure of Pseudomonas putida are not conserved among all members of this family, because the literature describes a general role in the isomerization of beta,gamma-unsaturated enones to their alpha,beta-isomers, and because of the presence of fairly distantly related paralogs in Campylobacter jejuni, the family is regarded as not necessarily uniform in function. [Energy metabolism, Other]


Pssm-ID: 129125  Cd Length: 63  Bit Score: 73.61  E-value: 1.07e-19
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 447069915   2 PYVTVKML-EGRTEEQKKALAEKVTAAVSETTGAPEENIVVFIEEMSKNHYAVGGKRLSD 60
Cdd:TIGR00013  1 PFVNIYILkEGRTDEQKRQLIEGVTEAMAETLGANLESIVVIIDEMPKNNYGIGGELVSD 60
 
Name Accession Description Interval E-value
PRK02220 PRK02220
4-oxalocrotonate tautomerase; Provisional
 1-61 1.77e-29

4-oxalocrotonate tautomerase; Provisional


Pssm-ID: 235013  Cd Length: 61  Bit Score: 98.30  E-value: 1.77e-29
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 447069915  1 MPYVTVKMLEGRTEEQKKALAEKVTAAVSETTGAPEENIVVFIEEMSKNHYAVGGKRLSDK 61
Cdd:PRK02220  1 MPYVHIKLIEGRTEEQLKALVKDVTAAVSKNTGAPAEHIHVIINEMSKNHYAVGGKRLSDK 61
4Oxalocrotonate_Tautomerase cd00491
4-Oxalocrotonate Tautomerase: Catalyzes the isomerization of unsaturated ketones. The ...
 2-59 1.45e-23

4-Oxalocrotonate Tautomerase: Catalyzes the isomerization of unsaturated ketones. The structure is a homohexamer that is arranged as a trimer of dimers. The hexamer contains six active sites, each formed by residues from three monomers, two from one dimer and the third from a neighboring monomer. Each monomer is a beta-alpha-beta fold with two small beta strands at the C-terminus that fold back on themselves. A pair of monomers form a dimer with two-fold symmetry, consisting of a 4-stranded beta sheet with two helices on one side and two additional small beta strands at each end. The dimers are assembled around a 3-fold axis of rotation to form a hexamer, with the short beta strands from each dimer contacting the neighboring dimers.


Pssm-ID: 238274  Cd Length: 58  Bit Score: 83.21  E-value: 1.45e-23
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 447069915  2 PYVTVKMLEGRTEEQKKALAEKVTAAVSETTGAPEENIVVFIEEMSKNHYAVGGKRLS 59
Cdd:cd00491   1 PFVQIYILEGRTDEQKRELIERVTEAVSEILGAPEATIVVIIDEMPKENWGIGGESAS 58
PptA COG1942
Phenylpyruvate tautomerase PptA, 4-oxalocrotonate tautomerase family [Secondary metabolites ...
 2-59 2.60e-23

Phenylpyruvate tautomerase PptA, 4-oxalocrotonate tautomerase family [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 441545  Cd Length: 58  Bit Score: 82.50  E-value: 2.60e-23
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 447069915  2 PYVTVKMLEGRTEEQKKALAEKVTAAVSETTGAPEENIVVFIEEMSKNHYAVGGKRLS 59
Cdd:COG1942   1 PIINIKLFEGRTPEQKRALAKAVTDAVVEVLGKPPESVSVVIEEVPPENWGIGGKLLS 58
Tautomerase pfam01361
Tautomerase enzyme; This family includes the enzyme 4-oxalocrotonate tautomerase that ...
 2-61 2.63e-22

Tautomerase enzyme; This family includes the enzyme 4-oxalocrotonate tautomerase that catalyzes the ketonization of 2-hydroxymuconate to 2-oxo-3-hexenedioate.


Pssm-ID: 396090  Cd Length: 60  Bit Score: 80.03  E-value: 2.63e-22
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 447069915   2 PYVTVKMLEGRTEEQKKALAEKVTAAVSETTGAPEENIVVFIEEMSKNHYAVGGKRLSDK 61
Cdd:pfam01361  1 PFVNIKLFEGRTDEQKKELIERVTDALVEVLGAPRSAIVVIIEEVPPENWGVGGESVGDR 60
taut TIGR00013
4-oxalocrotonate tautomerase family enzyme; 4-oxalocrotonate tautomerase is a homohexamer in ...
 2-60 1.07e-19

4-oxalocrotonate tautomerase family enzyme; 4-oxalocrotonate tautomerase is a homohexamer in which each monomer is very small, at about 62 amino acids. Pro-1 of the mature protein serves as a general base. The enzyme functions in meta-cleavage pathways of aromatic hydrocarbon catabolism. Because several Arg residues located near the active site in the crystal structure of Pseudomonas putida are not conserved among all members of this family, because the literature describes a general role in the isomerization of beta,gamma-unsaturated enones to their alpha,beta-isomers, and because of the presence of fairly distantly related paralogs in Campylobacter jejuni, the family is regarded as not necessarily uniform in function. [Energy metabolism, Other]


Pssm-ID: 129125  Cd Length: 63  Bit Score: 73.61  E-value: 1.07e-19
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 447069915   2 PYVTVKML-EGRTEEQKKALAEKVTAAVSETTGAPEENIVVFIEEMSKNHYAVGGKRLSD 60
Cdd:TIGR00013  1 PFVNIYILkEGRTDEQKRQLIEGVTEAMAETLGANLESIVVIIDEMPKNNYGIGGELVSD 60
PRK02289 PRK02289
4-oxalocrotonate tautomerase; Provisional
 1-51 2.44e-14

4-oxalocrotonate tautomerase; Provisional


Pssm-ID: 179406  Cd Length: 60  Bit Score: 59.95  E-value: 2.44e-14
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|.
gi 447069915  1 MPYVTVKMLEGRTEEQKKALAEKVTAAVSETTGAPEENIVVFIEEMSKNHY 51
Cdd:PRK02289  1 MPFVRIDLFEGRSQEQKNALAREVTEVVSRIAKAPKEAIHVFINDMPEGTY 51
PRK01964 PRK01964
4-oxalocrotonate tautomerase; Provisional
 1-61 1.56e-13

4-oxalocrotonate tautomerase; Provisional


Pssm-ID: 179355  Cd Length: 64  Bit Score: 58.27  E-value: 1.56e-13
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 447069915  1 MPYVTVKMLEGRTEEQKKALAEKVTAAVSETTGAPEENIVVFIEEMSKNHYAVGGKRLSDK 61
Cdd:PRK01964  1 MPIVQIQLLEGRPEEKIKNLIREVTEAISATLDVPKERVRVIVNEVPSSHWGVAGVPKSEL 61
PRK00745 PRK00745
4-oxalocrotonate tautomerase; Provisional
 1-61 7.46e-13

4-oxalocrotonate tautomerase; Provisional


Pssm-ID: 179107  Cd Length: 62  Bit Score: 56.27  E-value: 7.46e-13
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 447069915  1 MPYVTVKMLEGRTEEQKKALAEKVTAAVSETTGAPEENIVVFIEEMSKNHYAVGGKRLSDK 61
Cdd:PRK00745  1 MPTFHIELFEGRTVEQKRKLVEEITRVTVETLGCPPESVDIIITDVKRENWATGGKLWSDP 61
HpaF COG3232
5-carboxymethyl-2-hydroxymuconate isomerase [Amino acid transport and metabolism];
3-52 6.54e-10

5-carboxymethyl-2-hydroxymuconate isomerase [Amino acid transport and metabolism];


Pssm-ID: 442464 [Multi-domain]  Cd Length: 121  Bit Score: 50.24  E-value: 6.54e-10
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 447069915   3 YVTVKMLEGRTEEQKKALAEKVTAAVSETTGAPEENIV---VFIEEMSKNHYA 52
Cdd:COG3232   62 HVTLRILAGRSDEQKQALSEALFAALRAHLAPVFARYValsVEIREIDRASYA 114
CHMI cd00580
5-carboxymethyl-2-hydroxymuconate isomerase (CHMI) is a trimeric enzyme catalyzing the ...
 3-48 2.07e-07

5-carboxymethyl-2-hydroxymuconate isomerase (CHMI) is a trimeric enzyme catalyzing the isomerization of the unsaturated ketone 5-(carboxymethyl)-2-hydroxymuconate to 5-(carboxymethyl)-2-oxo-3-hexene-1,6-dionate. This is one step in the homoprotocatechuate pathway, one of the microbial meta-fission pathways that degrade aromatic carbon sources to citric acid cycle intermediates. Despite the structural similarity of CHMI with 4-oxalocrotonate tautomerase (4-OT) and macrophage migration inhibitory factor (MIF), there is no significant sequence similarity among these protein families, and therefore, they are not combined in one hierarchy.


Pssm-ID: 238324 [Multi-domain]  Cd Length: 113  Bit Score: 43.71  E-value: 2.07e-07
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 447069915   3 YVTVKMLEGRTEEQKKALAEKVTAAVSETTGAPEENIVVF----IEEMSK 48
Cdd:cd00580   62 HVTLRILAGRSEEQKQELSEALLAALRAHLAPVFAKRYLSlsveIRELDP 111
Tautomerase_2 pfam14552
Tautomerase enzyme;
3-45 1.01e-04

Tautomerase enzyme;


Pssm-ID: 434035  Cd Length: 82  Bit Score: 36.00  E-value: 1.01e-04
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|...
gi 447069915   3 YVTVKMLEGRTEEQKKALAEKVTAAVSETTGAPEENIVVFIEE 45
Cdd:pfam14552 31 IIQITLFAGRSVEQKKALYRALAERLAAKLGIRPEDVFIVLVE 73
Tautomerase_3 pfam14832
Putative oxalocrotonate tautomerase enzyme; 4-oxalocrotonate tautomerase enzyme is involved in ...
 13-61 7.22e-04

Putative oxalocrotonate tautomerase enzyme; 4-oxalocrotonate tautomerase enzyme is involved in the anthranilate synthase pathway.1


Pssm-ID: 434251 [Multi-domain]  Cd Length: 136  Bit Score: 34.59  E-value: 7.22e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 447069915   13 TEEQKKALAEKVTAaVSETTGAPEENIVVFIEEMSKNHYAVGGKRLSDK 61
Cdd:pfam14832  13 TDEEKQALAASITK-IYTSVGLPAFYVVVNFIEIPPSDFFVGGEPHPEN 60
CHMI pfam02962
5-carboxymethyl-2-hydroxymuconate isomerase;
3-30 2.10e-03

5-carboxymethyl-2-hydroxymuconate isomerase;


Pssm-ID: 427080  Cd Length: 124  Bit Score: 33.24  E-value: 2.10e-03
                          10        20
                  ....*....|....*....|....*...
gi 447069915    3 YVTVKMLEGRTEEQKKALAEKVTAAVSE 30
Cdd:pfam02962  62 HLTLKIGAGRSEEVKKAAGDALFAAIKD 89
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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