|
Name |
Accession |
Description |
Interval |
E-value |
| PRK00174 |
PRK00174 |
acetyl-CoA synthetase; Provisional |
4-640 |
0e+00 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 234677 [Multi-domain] Cd Length: 637 Bit Score: 1289.33 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 4 IYPVPEEFKKTARTVEADYFKRYQHSIENPDEFWAEQAKIVDWIKPFtqvkNTSFDKDNFKIEWFADGELNVSANCLDRH 83
Cdd:PRK00174 1 VFPPPAEFAANALIDMEQYKALYQESVEDPEGFWAEQAKRLDWFKPF----DTVLDWNAPFIKWFEDGELNVSYNCLDRH 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 84 LKEHPHKPAIIWEGDHPSRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGG 163
Cdd:PRK00174 77 LKTRGDKVAIIWEGDDPGDSRKITYRELHREVCRFANALKSLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVVFGG 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 164 FSPDSLASRIEDSQAKLVITADSSLRAGKLLPLKENVDLALALPgtECVENVIVVYRNANPIEMKPGRDLWYHLIIMEVD 243
Cdd:PRK00174 157 FSAEALADRIIDAGAKLVITADEGVRGGKPIPLKANVDEALANC--PSVEKVIVVRRTGGDVDWVEGRDLWWHELVAGAS 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 244 ANCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGT 323
Cdd:PRK00174 235 DECEPEPMDAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAAMTMKYVFDYKDGDVYWCTADVGWVTGHSYIVYGPLANGA 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 324 TTLMFEGVPQYPTWARLGHVVDKHKVSILYTAPTAIRAMMREGDSYVRESNRSSLRLLGSVGEPINPEAWNWYYNVVGEG 403
Cdd:PRK00174 315 TTLMFEGVPNYPDPGRFWEVIDKHKVTIFYTAPTAIRALMKEGDEHPKKYDLSSLRLLGSVGEPINPEAWEWYYKVVGGE 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 404 RCPIVDTWWQTETGGILIAPLPGATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDSWPGQMRTIWGDPDRFI 483
Cdd:PRK00174 395 RCPIVDTWWQTETGGIMITPLPGATPLKPGSATRPLPGIQPAVVDEEGNPLEGGEGGNLVIKDPWPGMMRTIYGDHERFV 474
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 484 EAYFSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTL 563
Cdd:PRK00174 475 KTYFSTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKVAEAAVVGRPDDIKGQGIYAFVTL 554
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446925704 564 QAGVPESEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKIAANELdSLGDTSTLAEPAVVDQLI 640
Cdd:PRK00174 555 KGGEEPSDELRKELRNWVRKEIGPIAKPDVIQFAPGLPKTRSGKIMRRILRKIAEGEE-ILGDTSTLADPSVVEKLI 630
|
|
| ACS |
cd05966 |
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ... |
21-632 |
0e+00 |
|
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.
Pssm-ID: 341270 [Multi-domain] Cd Length: 608 Bit Score: 1148.07 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 21 DYFKRYQHSIENPDEFWAEQAKIVDWIKPFTQVKNTSFDKDNFKieWFADGELNVSANCLDRHLKEHPHKPAIIWEGDHP 100
Cdd:cd05966 2 QYKELYKQSIEDPEEFWGEIAKELDWFKPWDKVLDWSKGPPFIK--WFEGGKLNISYNCLDRHLKERGDKVAIIWEGDEP 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 101 SRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKL 180
Cdd:cd05966 80 DQSRTITYRELLREVCRFANVLKSLGVKKGDRVAIYMPMIPELVIAMLACARIGAVHSVVFAGFSAESLADRINDAQCKL 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 181 VITADSSLRAGKLLPLKENVDLALAlpGTECVENVIVVYRNANPIEMKPGRDLWYHLIIMEVDANCPPEPMKAEDPLFIL 260
Cdd:cd05966 160 VITADGGYRGGKVIPLKEIVDEALE--KCPSVEKVLVVKRTGGEVPMTEGRDLWWHDLMAKQSPECEPEWMDSEDPLFIL 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 261 YTSGSTGKPKGVLHTTGGYLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFEGVPQYPTWARL 340
Cdd:cd05966 238 YTSGSTGKPKGVVHTTGGYLLYAATTFKYVFDYHPDDIYWCTADIGWITGHSYIVYGPLANGATTVMFEGTPTYPDPGRY 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 341 GHVVDKHKVSILYTAPTAIRAMMREGDSYVRESNRSSLRLLGSVGEPINPEAWNWYYNVVGEGRCPIVDTWWQTETGGIL 420
Cdd:cd05966 318 WDIVEKHKVTIFYTAPTAIRALMKFGDEWVKKHDLSSLRVLGSVGEPINPEAWMWYYEVIGKERCPIVDTWWQTETGGIM 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 421 IAPLPGATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDSWPGQMRTIWGDPDRFIEAYFSTFKNTYFTGDGA 500
Cdd:cd05966 398 ITPLPGATPLKPGSATRPFFGIEPAILDEEGNEVEGEVEGYLVIKRPWPGMARTIYGDHERYEDTYFSKFPGYYFTGDGA 477
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 501 RRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEELRKELISW 580
Cdd:cd05966 478 RRDEDGYYWITGRVDDVINVSGHRLGTAEVESALVAHPAVAEAAVVGRPHDIKGEAIYAFVTLKDGEEPSDELRKELRKH 557
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|..
gi 446925704 581 VRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKIAANELDsLGDTSTLAE 632
Cdd:cd05966 558 VRKEIGPIATPDKIQFVPGLPKTRSGKIMRRILRKIAAGEEE-LGDTSTLAD 608
|
|
| Ac_CoA_lig_AcsA |
TIGR02188 |
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called ... |
21-642 |
0e+00 |
|
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called acetyl-CoA synthetase and acetyl-activating enzyme. It catalyzes the reaction ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA and belongs to the family of AMP-binding enzymes described by pfam00501.
Pssm-ID: 274022 [Multi-domain] Cd Length: 626 Bit Score: 1135.82 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 21 DYFKRYQHSIENPDEFWAEQAK-IVDWIKPFTQVKNTSFDKDnfkIEWFADGELNVSANCLDRHLKEHPHKPAIIWEGDH 99
Cdd:TIGR02188 6 QYKELYEESIEDPDKFWAKLAReLLDWFKPFTKVLDWSFPPF---YKWFVGGELNVSYNCVDRHLEARPDKVAIIWEGDE 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 100 PSRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAK 179
Cdd:TIGR02188 83 PGEVRKITYRELHREVCRFANVLKSLGVKKGDRVAIYMPMIPEAAIAMLACARIGAIHSVVFGGFSAEALADRINDAGAK 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 180 LVITADSSLRAGKLLPLKENVDLALALPGTEcVENVIVVYRNANPI-EMKPGRDLWYHLIIMEVDANCPPEPMKAEDPLF 258
Cdd:TIGR02188 163 LVITADEGLRGGKVIPLKAIVDEALEKCPVS-VEHVLVVRRTGNPVvPWVEGRDVWWHDLMAKASAYCEPEPMDSEDPLF 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 259 ILYTSGSTGKPKGVLHTTGGYLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFEGVPQYPTWA 338
Cdd:TIGR02188 242 ILYTSGSTGKPKGVLHTTGGYLLYAAMTMKYVFDIKDGDIFWCTADVGWITGHSYIVYGPLANGATTVMFEGVPTYPDPG 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 339 RLGHVVDKHKVSILYTAPTAIRAMMREGDSYVRESNRSSLRLLGSVGEPINPEAWNWYYNVVGEGRCPIVDTWWQTETGG 418
Cdd:TIGR02188 322 RFWEIIEKHKVTIFYTAPTAIRALMRLGDEWVKKHDLSSLRLLGSVGEPINPEAWMWYYKVVGKERCPIVDTWWQTETGG 401
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 419 ILIAPLPGATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGN-LVIKDSWPGQMRTIWGDPDRFIEAYFSTFKNTYFTG 497
Cdd:TIGR02188 402 IMITPLPGATPTKPGSATLPFFGIEPAVVDEEGNPVEGPGEGGyLVIKQPWPGMLRTIYGDHERFVDTYFSPFPGYYFTG 481
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 498 DGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEELRKEL 577
Cdd:TIGR02188 482 DGARRDKDGYIWITGRVDDVINVSGHRLGTAEIESALVSHPAVAEAAVVGIPDDIKGQAIYAFVTLKDGYEPDDELRKEL 561
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 446925704 578 ISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKIAANELDSLGDTSTLAEPAVVDQLITT 642
Cdd:TIGR02188 562 RKHVRKEIGPIAKPDKIRFVPGLPKTRSGKIMRRLLRKIAAGEAEILGDTSTLEDPSVVEELIEA 626
|
|
| Acs |
COG0365 |
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism]; |
66-640 |
0e+00 |
|
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
Pssm-ID: 440134 [Multi-domain] Cd Length: 565 Bit Score: 940.31 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 66 EWFADGELNVSANCLDRHLKEHPHKPAIIWEGDhPSRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAI 145
Cdd:COG0365 1 RWFVGGRLNIAYNCLDRHAEGRGDKVALIWEGE-DGEERTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAVI 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 146 AMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITADSSLRAGKLLPLKENVDLALAlpGTECVENVIVVYRNANPI 225
Cdd:COG0365 80 AMLACARIGAVHSPVFPGFGAEALADRIEDAEAKVLITADGGLRGGKVIDLKEKVDEALE--ELPSLEHVIVVGRTGADV 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 226 EMkPGrDLWYHLIIMEVDANCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVASTFKEVFDLKQDDVYWCTADV 305
Cdd:COG0365 158 PM-EG-DLDWDELLAAASAEFEPEPTDADDPLFILYTSGTTGKPKGVVHTHGGYLVHAATTAKYVLDLKPGDVFWCTADI 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 306 GWITGHSYLIYGPLANGTTTLMFEGVPQYPTWARLGHVVDKHKVSILYTAPTAIRAMMREGDSYVRESNRSSLRLLGSVG 385
Cdd:COG0365 236 GWATGHSYIVYGPLLNGATVVLYEGRPDFPDPGRLWELIEKYGVTVFFTAPTAIRALMKAGDEPLKKYDLSSLRLLGSAG 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 386 EPINPEAWNWYYNVVGegrCPIVDTWWQTETGGILIAPLPGaTALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIK 465
Cdd:COG0365 316 EPLNPEVWEWWYEAVG---VPIVDGWGQTETGGIFISNLPG-LPVKPGSMGKPVPGYDVAVVDEDGNPVPPGEEGELVIK 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 466 DSWPGQMRTIWGDPDRFIEAYFSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAV 545
Cdd:COG0365 392 GPWPGMFRGYWNDPERYRETYFGRFPGWYRTGDGARRDEDGYFWILGRSDDVINVSGHRIGTAEIESALVSHPAVAEAAV 471
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 546 VGMPHDIKGQGICTFVTLQAGVPESEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKIAANEldSLG 625
Cdd:COG0365 472 VGVPDEIRGQVVKAFVVLKPGVEPSDELAKELQAHVREELGPYAYPREIEFVDELPKTRSGKIMRRLLRKIAEGR--PLG 549
|
570
....*....|....*
gi 446925704 626 DTSTLAEPAVVDQLI 640
Cdd:COG0365 550 DTSTLEDPEALDEIK 564
|
|
| ACS-like |
cd17634 |
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ... |
22-609 |
0e+00 |
|
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341289 [Multi-domain] Cd Length: 587 Bit Score: 841.08 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 22 YFKRYQHSIENPDEFWAEQAKIVDWIKPFTQVKNTSFDKDNFKIEWFADGELNVSANCLDRHLKEHPHKPAIIWEGDHPS 101
Cdd:cd17634 1 YETKYRQSINDPDTFWGEAGKILDWITPYQKVKNTSFAPGAPSIKWFEDATLNLAANALDRHLRENGDRTAIIYEGDDTS 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 102 RHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLV 181
Cdd:cd17634 81 QSRTISYRELHREVCRFAGTLLDLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVIFGGFAPEAVAGRIIDSSSRLL 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 182 ITADSSLRAGKLLPLKENVDLALALPGTEcVENVIVVYRNANPIEMKPGRDLWYHLIIMEVDANCPPEPMKAEDPLFILY 261
Cdd:cd17634 161 ITADGGVRAGRSVPLKKNVDDALNPNVTS-VEHVIVLKRTGSDIDWQEGRDLWWRDLIAKASPEHQPEAMNAEDPLFILY 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 262 TSGSTGKPKGVLHTTGGYLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFEGVPQYPTWARLG 341
Cdd:cd17634 240 TSGTTGKPKGVLHTTGGYLVYAATTMKYVFDYGPGDIYWCTADVGWVTGHSYLLYGPLACGATTLLYEGVPNWPTPARMW 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 342 HVVDKHKVSILYTAPTAIRAMMREGDSYVRESNRSSLRLLGSVGEPINPEAWNWYYNVVGEGRCPIVDTWWQTETGGILI 421
Cdd:cd17634 320 QVVDKHGVNILYTAPTAIRALMAAGDDAIEGTDRSSLRILGSVGEPINPEAYEWYWKKIGKEKCPVVDTWWQTETGGFMI 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 422 APLPGATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDSWPGQMRTIWGDPDRFIEAYFSTFKNTYFTGDGAR 501
Cdd:cd17634 400 TPLPGAIELKAGSATRPVFGVQPAVVDNEGHPQPGGTEGNLVITDPWPGQTRTLFGDHERFEQTYFSTFKGMYFSGDGAR 479
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 502 RDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEELRKELISWV 581
Cdd:cd17634 480 RDEDGYYWITGRSDDVINVAGHRLGTAEIESVLVAHPKVAEAAVVGIPHAIKGQAPYAYVVLNHGVEPSPELYAELRNWV 559
|
570 580
....*....|....*....|....*...
gi 446925704 582 RKVLGPVASPDALHWAPALPKTRSGKIM 609
Cdd:cd17634 560 RKEIGPLATPDVVHWVDSLPKTRSGKIM 587
|
|
| PLN02654 |
PLN02654 |
acetate-CoA ligase |
4-640 |
0e+00 |
|
acetate-CoA ligase
Pssm-ID: 215353 [Multi-domain] Cd Length: 666 Bit Score: 838.40 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 4 IYPvPEEFKKTAR-TVEADYFKRYQHSIENPDEFWAEQAKIVDWIKPFTQVK----NTSFDKDNFKIEWFADGELNVSAN 78
Cdd:PLN02654 14 VFP-SKDFSAQALvSSPQQYMEMYKRSVDDPAGFWSDIASQFYWKQKWEGDEvcseNLDVRKGPISIEWFKGGKTNICYN 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 79 CLDRHLKE-HPHKPAIIWEGDHPSRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVH 157
Cdd:PLN02654 93 CLDRNVEAgNGDKIAIYWEGNEPGFDASLTYSELLDRVCQLANYLKDVGVKKGDAVVIYLPMLMELPIAMLACARIGAVH 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 158 CVVFGGFSPDSLASRIEDSQAKLVITADSSLRAGKLLPLKENVDLALAlpgtECVENVIVV-----YRNANPIEM----- 227
Cdd:PLN02654 173 SVVFAGFSAESLAQRIVDCKPKVVITCNAVKRGPKTINLKDIVDAALD----ESAKNGVSVgicltYENQLAMKRedtkw 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 228 KPGRDLWYHLIIMEVDANCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVASTFKEVFDLKQDDVYWCTADVGW 307
Cdd:PLN02654 249 QEGRDVWWQDVVPNYPTKCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPTDVYWCTADCGW 328
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 308 ITGHSYLIYGPLANGTTTLMFEGVPQYPTWARLGHVVDKHKVSILYTAPTAIRAMMREGDSYVRESNRSSLRLLGSVGEP 387
Cdd:PLN02654 329 ITGHSYVTYGPMLNGATVLVFEGAPNYPDSGRCWDIVDKYKVTIFYTAPTLVRSLMRDGDEYVTRHSRKSLRVLGSVGEP 408
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 388 INPEAWNWYYNVVGEGRCPIVDTWWQTETGGILIAPLPGATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDS 467
Cdd:PLN02654 409 INPSAWRWFFNVVGDSRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGKEIEGECSGYLCVKKS 488
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 468 WPGQMRTIWGDPDRFIEAYFSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVG 547
Cdd:PLN02654 489 WPGAFRTLYGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVG 568
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 548 MPHDIKGQGICTFVTLQAGVPESEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKIAANELDSLGDT 627
Cdd:PLN02654 569 IEHEVKGQGIYAFVTLVEGVPYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASRQLDELGDT 648
|
650
....*....|...
gi 446925704 628 STLAEPAVVDQLI 640
Cdd:PLN02654 649 STLADPGVVDQLI 661
|
|
| PrpE |
cd05967 |
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ... |
22-639 |
0e+00 |
|
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.
Pssm-ID: 341271 [Multi-domain] Cd Length: 617 Bit Score: 672.10 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 22 YFKRYQHSIENPDEFWAEQAKIVDWIKPFTQVkntsFDKDNFKI-EWFADGELNVSANCLDRHLKE-HPHKPAIIWEGDH 99
Cdd:cd05967 1 YEEVYARSIAEPEAFWAEQARLIDWFKPPEKI----LDNSNPPFtRWFVGGRLNTCYNALDRHVEAgRGDQIALIYDSPV 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 100 PSRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAK 179
Cdd:cd05967 77 TGTERTYTYAELLDEVSRLAGVLRKLGVVKGDRVIIYMPMIPEAAIAMLACARIGAIHSVVFGGFAAKELASRIDDAKPK 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 180 LVITADSSLRAGKLLPLKENVDLALALPGTEcVENVIVVYRNANPIEM-KPGRDL-WYHLIIMEVDANCPpePMKAEDPL 257
Cdd:cd05967 157 LIVTASCGIEPGKVVPYKPLLDKALELSGHK-PHHVLVLNRPQVPADLtKPGRDLdWSELLAKAEPVDCV--PVAATDPL 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 258 FILYTSGSTGKPKGVLHTTGGYLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFEG----VPQ 333
Cdd:cd05967 234 YILYTSGTTGKPKGVVRDNGGHAVALNWSMRNIYGIKPGDVWWAASDVGWVVGHSYIVYGPLLHGATTVLYEGkpvgTPD 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 334 YPTWARlghVVDKHKVSILYTAPTAIRAMMRE--GDSYVRESNRSSLRLLGSVGEPINPEAWNWYYNVVGEgrcPIVDTW 411
Cdd:cd05967 314 PGAFWR---VIEKYQVNALFTAPTAIRAIRKEdpDGKYIKKYDLSSLRTLFLAGERLDPPTLEWAENTLGV---PVIDHW 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 412 WQTETGGILIAPLPG--ATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDSW-PGQMRTIWGDPDRFIEAYFS 488
Cdd:cd05967 388 WQTETGWPITANPVGlePLPIKAGSPGKPVPGYQVQVLDEDGEPVGPNELGNIVIKLPLpPGCLLTLWKNDERFKKLYLS 467
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 489 TFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVP 568
Cdd:cd05967 468 KFPGYYDTGDAGYKDEDGYLFIMGRTDDVINVAGHRLSTGEMEESVLSHPAVAECAVVGVRDELKGQVPLGLVVLKEGVK 547
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 446925704 569 ES-EELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKIAANEldSLGDTSTLAEPAVVDQL 639
Cdd:cd05967 548 ITaEELEKELVALVREQIGPVAAFRLVIFVKRLPKTRSGKILRRTLRKIADGE--DYTIPSTIEDPSVLDEI 617
|
|
| prpE |
PRK10524 |
propionyl-CoA synthetase; Provisional |
26-639 |
0e+00 |
|
propionyl-CoA synthetase; Provisional
Pssm-ID: 182517 [Multi-domain] Cd Length: 629 Bit Score: 633.52 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 26 YQHSIENPDEFWAEQAKIVDWIKPFTQVKNTS---FDKdnfkieWFADGELNVSANCLDRHLKEHPHKPAIIWEGDHPSR 102
Cdd:PRK10524 8 YQRSIDDPEAFWAEQARRIDWQTPFTQVLDYSnppFAR------WFVGGRTNLCHNAVDRHLAKRPEQLALIAVSTETDE 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 103 HKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVI 182
Cdd:PRK10524 82 ERTYTFRQLHDEVNRMAAMLRSLGVQRGDRVLIYMPMIAEAAFAMLACARIGAIHSVVFGGFASHSLAARIDDAKPVLIV 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 183 TADSSLRAGKLLPLKENVDLALALPGTEcVENVIVVYRNANPIEMKPGRDLWY------HLiimevDANCPPEPMKAEDP 256
Cdd:PRK10524 162 SADAGSRGGKVVPYKPLLDEAIALAQHK-PRHVLLVDRGLAPMARVAGRDVDYatlraqHL-----GARVPVEWLESNEP 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 257 LFILYTSGSTGKPKGVLHTTGGYLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFEGVPQYP- 335
Cdd:PRK10524 236 SYILYTSGTTGKPKGVQRDTGGYAVALATSMDTIFGGKAGETFFCASDIGWVVGHSYIVYAPLLAGMATIMYEGLPTRPd 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 336 --TWARLghvVDKHKVSILYTAPTAIRAMMREGDSYVRESNRSSLRLLGSVGEPINPEAWNWYYNVVGEgrcPIVDTWWQ 413
Cdd:PRK10524 316 agIWWRI---VEKYKVNRMFSAPTAIRVLKKQDPALLRKHDLSSLRALFLAGEPLDEPTASWISEALGV---PVIDNYWQ 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 414 TETGGILIAPLPG--ATALKPGSATRPLFGIQPAIVDGE-GNELEGAAEGNLVIKDSW-PGQMRTIWGDPDRFIEAYFST 489
Cdd:PRK10524 390 TETGWPILAIARGveDRPTRLGSPGVPMYGYNVKLLNEVtGEPCGPNEKGVLVIEGPLpPGCMQTVWGDDDRFVKTYWSL 469
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 490 F-KNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTL-QAGV 567
Cdd:PRK10524 470 FgRQVYSTFDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEESISSHPAVAEVAVVGVKDALKGQVAVAFVVPkDSDS 549
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446925704 568 PESEELR----KELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKIAanELDSLGDTSTLAEPAVVDQL 639
Cdd:PRK10524 550 LADREARlaleKEIMALVDSQLGAVARPARVWFVSALPKTRSGKLLRRAIQAIA--EGRDPGDLTTIEDPAALQQI 623
|
|
| AACS_like |
cd05968 |
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ... |
20-633 |
0e+00 |
|
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.
Pssm-ID: 341272 [Multi-domain] Cd Length: 610 Bit Score: 566.73 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 20 ADYFKRYQHSIENPDEFWAEQAKIVD--WIKPFTQVKNTSFDKDNFKieWFADGELNVSANCLDRHLKEHPHKPAIIWEG 97
Cdd:cd05968 7 PDLEAFLERSAEDNAWFWGEFVKDVGieWYEPPYQTLDLSGGKPWAA--WFVGGRMNIVEQLLDKWLADTRTRPALRWEG 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 98 DHpSRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQ 177
Cdd:cd05968 85 ED-GTSRTLTYGELLYEVKRLANGLRALGVGKGDRVGIYLPMIPEIVPAFLAVARIGGIVVPIFSGFGKEAAATRLQDAE 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 178 AKLVITADSSLRAGKLLPLKENVDLALALPGTecVENVIVVYRNANPIEMKPGRDLWYHLIIMEVDANCppEPMKAEDPL 257
Cdd:cd05968 164 AKALITADGFTRRGREVNLKEEADKACAQCPT--VEKVVVVRHLGNDFTPAKGRDLSYDEEKETAGDGA--ERTESEDPL 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 258 FILYTSGSTGKPKGVLHTTGGYLVYVASTFKEVFDLKQDD-VYWCTaDVGWITGhSYLIYGPLANGTTTLMFEGVPQYPT 336
Cdd:cd05968 240 MIIYTSGTTGKPKGTVHVHAGFPLKAAQDMYFQFDLKPGDlLTWFT-DLGWMMG-PWLIFGGLILGATMVLYDGAPDHPK 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 337 WARLGHVVDKHKVSILYTAPTAIRAMMREGDSYVRESNRSSLRLLGSVGEPINPEAWNWYYNVVGEGRCPIVDTWWQTE- 415
Cdd:cd05968 318 ADRLWRMVEDHEITHLGLSPTLIRALKPRGDAPVNAHDLSSLRVLGSTGEPWNPEPWNWLFETVGKGRNPIINYSGGTEi 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 416 TGGIL----IAPLpgatalKPGSATRPLFGIQPAIVDGEGNELEGAaEGNLVIKDSWPGQMRTIWGDPDRFIEAYFSTFK 491
Cdd:cd05968 398 SGGILgnvlIKPI------KPSSFNGPVPGMKADVLDESGKPARPE-VGELVLLAPWPGMTRGFWRDEDRYLETYWSRFD 470
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 492 NTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESE 571
Cdd:cd05968 471 NVWVHGDFAYYDEEGYFYILGRSDDTINVAGKRVGPAEIESVLNAHPAVLESAAIGVPHPVKGEAIVCFVVLKPGVTPTE 550
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 446925704 572 ELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKIAANEldSLGDTSTLAEP 633
Cdd:cd05968 551 ALAEELMERVADELGKPLSPERILFVKDLPKTRNAKVMRRVIRAAYLGK--ELGDLSSLENP 610
|
|
| PRK04319 |
PRK04319 |
acetyl-CoA synthetase; Provisional |
65-630 |
0e+00 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 235279 [Multi-domain] Cd Length: 570 Bit Score: 540.64 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 65 IEWFADGELNVSANCLDRHLK-EHPHKPAIIWEGDHpsRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEA 143
Cdd:PRK04319 34 FSWLETGKVNIAYEAIDRHADgGRKDKVALRYLDAS--RKEKYTYKELKELSNKFANVLKELGVEKGDRVFIFMPRIPEL 111
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 144 AIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITADSSLRAgklLPLKEnvdlalaLPgteCVENVIVVyrnAN 223
Cdd:PRK04319 112 YFALLGALKNGAIVGPLFEAFMEEAVRDRLEDSEAKVLITTPALLER---KPADD-------LP---SLKHVLLV---GE 175
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 224 PIEMKPGrDLWYHLIIMEVDANCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVASTfKEVFDLKQDDVYWCTA 303
Cdd:PRK04319 176 DVEEGPG-TLDFNALMEQASDEFDIEWTDREDGAILHYTSGSTGKPKGVLHVHNAMLQHYQTG-KYVLDLHEDDVYWCTA 253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 304 DVGWITGHSYLIYGPLANGTTTLMFEGVPQYPTWARlghVVDKHKVSILYTAPTAIRAMMREGDSYVRESNRSSLRLLGS 383
Cdd:PRK04319 254 DPGWVTGTSYGIFAPWLNGATNVIDGGRFSPERWYR---ILEDYKVTVWYTAPTAIRMLMGAGDDLVKKYDLSSLRHILS 330
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 384 VGEPINPEAWNWYYNVVGEgrcPIVDTWWQTETGGILIAPLPgATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLV 463
Cdd:PRK04319 331 VGEPLNPEVVRWGMKVFGL---PIHDNWWMTETGGIMIANYP-AMDIKPGSMGKPLPGIEAAIVDDQGNELPPNRMGNLA 406
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 464 IKDSWPGQMRTIWGDPDRFiEAYFStfKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEA 543
Cdd:PRK04319 407 IKKGWPSMMRGIWNNPEKY-ESYFA--GDWYVSGDSAYMDEDGYFWFQGRVDDVIKTSGERVGPFEVESKLMEHPAVAEA 483
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 544 AVVGMPHDIKGQGICTFVTLQAGVPESEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRkiaANELD- 622
Cdd:PRK04319 484 GVIGKPDPVRGEIIKAFVALRPGYEPSEELKEEIRGFVKKGLGAHAAPREIEFKDKLPKTRSGKIMRRVLK---AWELGl 560
|
....*...
gi 446925704 623 SLGDTSTL 630
Cdd:PRK04319 561 PEGDLSTM 568
|
|
| MACS_like_4 |
cd05969 |
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ... |
107-617 |
5.14e-158 |
|
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.
Pssm-ID: 341273 [Multi-domain] Cd Length: 442 Bit Score: 461.97 E-value: 5.14e-158
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 107 SYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITADS 186
Cdd:cd05969 2 TFAQLKVLSARFANVLKSLGVGKGDRVFVLSPRSPELYFSMLGIGKIGAVICPLFSAFGPEAIRDRLENSEAKVLITTEE 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 187 SLragkllplkenvdlalalpgtecvenvivvyrnanpiemkpgrdlwyhliimevdancppEPMKAEDPLFILYTSGST 266
Cdd:cd05969 82 LY------------------------------------------------------------ERTDPEDPTLLHYTSGTT 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 267 GKPKGVLHTTGGYLVYvASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFEGVPQYPTWArlgHVVDK 346
Cdd:cd05969 102 GTPKGVLHVHDAMIFY-YFTGKYVLDLHPDDIYWCTADPGWVTGTVYGIWAPWLNGVTNVVYEGRFDAESWY---GIIER 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 347 HKVSILYTAPTAIRAMMREGDSYVRESNRSSLRLLGSVGEPINPEAWNWYYNVVGegrCPIVDTWWQTETGGILIAPLPG 426
Cdd:cd05969 178 VKVTVWYTAPTAIRMLMKEGDELARKYDLSSLRFIHSVGEPLNPEAIRWGMEVFG---VPIHDTWWQTETGSIMIANYPC 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 427 ATAlKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDSWPGQMRTIWGDPDRFiEAYFSTfkNTYFTGDGARRDEDG 506
Cdd:cd05969 255 MPI-KPGSMGKPLPGVKAAVVDENGNELPPGTKGILALKPGWPSMFRGIWNDEERY-KNSFID--GWYLTGDLAYRDEDG 330
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 507 YYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEELRKELISWVRKVLG 586
Cdd:cd05969 331 YFWFVGRADDIIKTSGHRVGPFEVESALMEHPAVAEAGVIGKPDPLRGEIIKAFISLKEGFEPSDELKEEIINFVRQKLG 410
|
490 500 510
....*....|....*....|....*....|.
gi 446925704 587 PVASPDALHWAPALPKTRSGKIMRRILRKIA 617
Cdd:cd05969 411 AHVAPREIEFVDNLPKTRSGKIMRRVLKAKE 441
|
|
| MACS_like |
cd05972 |
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ... |
106-615 |
1.27e-127 |
|
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.
Pssm-ID: 341276 [Multi-domain] Cd Length: 428 Bit Score: 383.23 E-value: 1.27e-127
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 106 VSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITad 185
Cdd:cd05972 1 WSFRELKRESAKAANVLAKLGLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAIVT-- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 186 sslragkllplkenvdlalalpgtecvenvivvyrnanpiemkpgrdlwyhliimevdancppepmKAEDPLFILYTSGS 265
Cdd:cd05972 79 ------------------------------------------------------------------DAEDPALIYFTSGT 92
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 266 TGKPKGVLHTTGgYLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFEGVPQYPTwaRLGHVVD 345
Cdd:cd05972 93 TGLPKGVLHTHS-YPLGHIPTAAYWLGLRPDDIHWNIADPGWAKGAWSSFFGPWLLGATVFVYEGPRFDAE--RILELLE 169
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 346 KHKVSILYTAPTAIRAMMREG-DSYVResnrSSLRLLGSVGEPINPEAWNWYYNVVGEgrcPIVDTWWQTETGgILIAPL 424
Cdd:cd05972 170 RYGVTSFCGPPTAYRMLIKQDlSSYKF----SHLRLVVSAGEPLNPEVIEWWRAATGL---PIRDGYGQTETG-LTVGNF 241
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 425 PGaTALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDSWPGQMRTIWGDPDR----FIEAYfstfkntYFTGDGA 500
Cdd:cd05972 242 PD-MPVKPGSMGRPTPGYDVAIIDDDGRELPPGEEGDIAIKLPPPGLFLGYVGDPEKteasIRGDY-------YLTGDRA 313
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 501 RRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEELRKELISW 580
Cdd:cd05972 314 YRDEDGYFWFVGRADDIIKSSGYRIGPFEVESALLEHPAVAEAAVVGSPDPVRGEVVKAFVVLTSGYEPSEELAEELQGH 393
|
490 500 510
....*....|....*....|....*....|....*
gi 446925704 581 VRKVLGPVASPDALHWAPALPKTRSGKIMRRILRK 615
Cdd:cd05972 394 VKKVLAPYKYPREIEFVEELPKTISGKIRRVELRD 428
|
|
| AMP-binding |
pfam00501 |
AMP-binding enzyme; |
80-521 |
1.33e-110 |
|
AMP-binding enzyme;
Pssm-ID: 459834 [Multi-domain] Cd Length: 417 Bit Score: 339.29 E-value: 1.33e-110
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIwegdhPSRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCV 159
Cdd:pfam00501 1 LERQAARTPDKTALE-----VGEGRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVP 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFGGFSPDSLASRIEDSQAKLVITaDSSLRAGKLLPLKENVdlalalpgtECVENVIVVYRNANPIEmkpgrDLWYHLII 239
Cdd:pfam00501 76 LNPRLPAEELAYILEDSGAKVLIT-DDALKLEELLEALGKL---------EVVKLVLVLDRDPVLKE-----EPLPEEAK 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 240 MEVDANCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTgGYLVYVASTFKEV----FDLKQDDVYWCTADVGWITGHSYLI 315
Cdd:pfam00501 141 PADVPPPPPPPPDPDDLAYIIYTSGTTGKPKGVMLTH-RNLVANVLSIKRVrprgFGLGPDDRVLSTLPLFHDFGLSLGL 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 316 YGPLANGTTTLMFEGVPQYPTWARLGhVVDKHKVSILYTAPTAIRAMMREGDsyVRESNRSSLRLLGSVGEPINPEAWNW 395
Cdd:pfam00501 220 LGPLLAGATVVLPPGFPALDPAALLE-LIERYKVTVLYGVPTLLNMLLEAGA--PKRALLSSLRLVLSGGAPLPPELARR 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 396 YYNVVGegrCPIVDTWWQTETGGILIAPLPGATAL-KPGSATRPLFGIQPAIVDGE-GNELEGAAEGNLVIKDswPGQMR 473
Cdd:pfam00501 297 FRELFG---GALVNGYGLTETTGVVTTPLPLDEDLrSLGSVGRPLPGTEVKIVDDEtGEPVPPGEPGELCVRG--PGVMK 371
|
410 420 430 440
....*....|....*....|....*....|....*....|....*...
gi 446925704 474 TIWGDPDRFIEAYFStfKNTYFTGDGARRDEDGYYWITGRVDDVLNVS 521
Cdd:pfam00501 372 GYLNDPELTAEAFDE--DGWYRTGDLGRRDEDGYLEIVGRKKDQIKLG 417
|
|
| MenE/FadK |
COG0318 |
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ... |
80-618 |
1.53e-106 |
|
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis
Pssm-ID: 440087 [Multi-domain] Cd Length: 452 Bit Score: 329.85 E-value: 1.53e-106
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCV 159
Cdd:COG0318 5 LRRAAARHPDRPALVFGGRR------LTYAELDARARRLAAALRALGVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVP 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFGGFSPDSLASRIEDSQAKLVITAdsslragkllplkenvdlalalpgtecvenvivvyrnanpiemkpgrdlwyhlii 239
Cdd:COG0318 79 LNPRLTAEELAYILEDSGARALVTA------------------------------------------------------- 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 240 mevdancppepmkaedplFILYTSGSTGKPKGVLHTTGGyLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPL 319
Cdd:COG0318 104 ------------------LILYTSGTTGRPKGVMLTHRN-LLANAAAIAAALGLTPGDVVLVALPLFHVFGLTVGLLAPL 164
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 320 ANGTTTLMFEGVpQYPTWARLghvVDKHKVSILYTAPTAIRAMMREGDsyVRESNRSSLRLLGSVGEPINPEAWNWYYNV 399
Cdd:COG0318 165 LAGATLVLLPRF-DPERVLEL---IERERVTVLFGVPTMLARLLRHPE--FARYDLSSLRLVVSGGAPLPPELLERFEER 238
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 400 VGegrCPIVDTWWQTETGGILIAPLPGATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDswPGQMRTIWGDP 479
Cdd:COG0318 239 FG---VRIVEGYGLTETSPVVTVNPEDPGERRPGSVGRPLPGVEVRIVDEDGRELPPGEVGEIVVRG--PNVMKGYWNDP 313
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 480 DRFIEAyfstFKNTYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGIC 558
Cdd:COG0318 314 EATAEA----FRDGWLrTGDLGRLDEDGYLYIVGRKKDMIISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKWGERVV 389
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 559 TFVTLQAGVPESEElrkELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKIAA 618
Cdd:COG0318 390 AFVVLRPGAELDAE---ELRAFLRERLARYKVPRRVEFVDELPRTASGKIDRRALRERYA 446
|
|
| AACS |
cd05943 |
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ... |
20-616 |
9.29e-96 |
|
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.
Pssm-ID: 341265 [Multi-domain] Cd Length: 629 Bit Score: 307.28 E-value: 9.29e-96
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 20 ADYFKRYQHSIENPDEFWAEqakIVDW--IKPFTQVKNTSFDKDNFKI-EWFADGELNVsANCLDRHlKEHPHKPAIIWE 96
Cdd:cd05943 17 ADYAALHRWSVDDPGAFWAA---VWDFsgVRGSKPYDVVVVSGRIMPGaRWFPGARLNY-AENLLRH-ADADDPAAIYAA 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 97 GDHPSRHkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDS 176
Cdd:cd05943 92 EDGERTE--VTWAELRRRVARLAAALRALGVKPGDRVAGYLPNIPEAVVAMLATASIGAIWSSCSPDFGVPGVLDRFGQI 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 177 QAKLVITADSSLRAGKLLPLKENV-DLALALPGTEcvENVIVVYRNANP---IEMKPGRDLWYHLIIMEVDANCPPEPMK 252
Cdd:cd05943 170 EPKVLFAVDAYTYNGKRHDVREKVaELVKGLPSLL--AVVVVPYTVAAGqpdLSKIAKALTLEDFLATGAAGELEFEPLP 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 253 AEDPLFILYTSGSTGKPKGVLHTTGGYLVYVASTFKEVFDLKQDDVYWCTADVGWITGHsYLIYGpLANGTTTLMFEGVP 332
Cdd:cd05943 248 FDHPLYILYSSGTTGLPKCIVHGAGGTLLQHLKEHILHCDLRPGDRLFYYTTCGWMMWN-WLVSG-LAVGATIVLYDGSP 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 333 QYPTWARLGHVVDKHKVSILYTAPTAIRAMMREGDSYVRESNRSSLRLLGSVGEPINPEAWNWYYNVVGEgrcpivDTWW 412
Cdd:cd05943 326 FYPDTNALWDLADEEGITVFGTSAKYLDALEKAGLKPAETHDLSSLRTILSTGSPLKPESFDYVYDHIKP------DVLL 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 413 QTETGGI-LIAPLPGATALKP---GSATRPLFGIQPAIVDGEGNELEGAAeGNLVIKDSWPGQMRTIWGDPD--RFIEAY 486
Cdd:cd05943 400 ASISGGTdIISCFVGGNPLLPvyrGEIQCRGLGMAVEAFDEEGKPVWGEK-GELVCTKPFPSMPVGFWNDPDgsRYRAAY 478
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 487 FSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAG 566
Cdd:cd05943 479 FAKYPGVWAHGDWIEITPRGGVVILGRSDGTLNPGGVRIGTAEIYRVVEKIPEVEDSLVVGQEWKDGDERVILFVKLREG 558
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|
gi 446925704 567 VPESEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKI 616
Cdd:cd05943 559 VELDDELRKRIRSTIRSALSPRHVPAKIIAVPDIPRTLSGKKVEVAVKKI 608
|
|
| AFD_class_I |
cd04433 |
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ... |
255-609 |
3.45e-93 |
|
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341228 [Multi-domain] Cd Length: 336 Bit Score: 291.11 E-value: 3.45e-93
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 255 DPLFILYTSGSTGKPKGVLHTTGGYLVYVASTFKeVFDLKQDDVYWCTADVGWItGHSYLIYGPLANGTTTLMFEGVPqY 334
Cdd:cd04433 1 DPALILYTSGTTGKPKGVVLSHRNLLAAAAALAA-SGGLTEGDVFLSTLPLFHI-GGLFGLLGALLAGGTVVLLPKFD-P 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 335 PTWARLghvVDKHKVSILYTAPTAIRAMMREGDSyvRESNRSSLRLLGSVGEPINPEAWNWYYNVVGegrCPIVDTWWQT 414
Cdd:cd04433 78 EAALEL---IEREKVTILLGVPTLLARLLKAPES--AGYDLSSLRALVSGGAPLPPELLERFEEAPG---IKLVNGYGLT 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 415 ETGGILIAPLPGATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDSWPgqMRTIWGDPDRfieAYFSTFKNTY 494
Cdd:cd04433 150 ETGGTVATGPPDDDARKPGSVGRPVPGVEVRIVDPDGGELPPGEIGELVVRGPSV--MKGYWNNPEA---TAAVDEDGWY 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 495 FTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEElr 574
Cdd:cd04433 225 RTGDLGRLDEDGYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEWGERVVAVVVLRPGADLDAE-- 302
|
330 340 350
....*....|....*....|....*....|....*
gi 446925704 575 kELISWVRKVLGPVASPDALHWAPALPKTRSGKIM 609
Cdd:cd04433 303 -ELRAHVRERLAPYKVPRRVVFVDALPRTASGKID 336
|
|
| PRK03584 |
PRK03584 |
acetoacetate--CoA ligase; |
20-632 |
2.15e-90 |
|
acetoacetate--CoA ligase;
Pssm-ID: 235134 [Multi-domain] Cd Length: 655 Bit Score: 294.01 E-value: 2.15e-90
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 20 ADYFKRYQHSIENPDEFWAEqakIVDWI-----KPFTQVKNtsfDKDNFKIEWFADGELNVSANCLdRHlkEHPHKPAII 94
Cdd:PRK03584 34 DDYAALWRWSVEDLEAFWQS---VWDFFgvigsTPYTVVLA---GRRMPGARWFPGARLNYAENLL-RH--RRDDRPAII 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 95 WEG-DHPSRhkIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRI 173
Cdd:PRK03584 105 FRGeDGPRR--ELSWAELRRQVAALAAALRALGVGPGDRVAAYLPNIPETVVAMLATASLGAIWSSCSPDFGVQGVLDRF 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 174 EDSQAKLVITADSSLRAGKLLPLKENV-DLALALPGtecVENVIVV--YRNANPIEMKPGRDLWYHLIIMEVDANCPPEP 250
Cdd:PRK03584 183 GQIEPKVLIAVDGYRYGGKAFDRRAKVaELRAALPS---LEHVVVVpyLGPAAAAAALPGALLWEDFLAPAEAAELEFEP 259
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 251 MKAEDPLFILYTSGSTGKPKGVLHTTGGYLVyvaSTFKEV---FDLKQDDVY-WCTAdVGWITgHSYLIYGpLANGTTTL 326
Cdd:PRK03584 260 VPFDHPLWILYSSGTTGLPKCIVHGHGGILL---EHLKELglhCDLGPGDRFfWYTT-CGWMM-WNWLVSG-LLVGATLV 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 327 MFEGVPQYPTWARLGHVVDKHKVSILYTAPTAIRAMMREGDSYVRESNRSSLRLLGSVGEPINPEAWNWYYNVVGEgrcp 406
Cdd:PRK03584 334 LYDGSPFYPDPNVLWDLAAEEGVTVFGTSAKYLDACEKAGLVPGETHDLSALRTIGSTGSPLPPEGFDWVYEHVKA---- 409
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 407 ivDTWWQTETGGILIAplpGATALkpGSATRPLF---------GIQPAIVDGEGNELEGAAeGNLVIKDSWPGQMRTIWG 477
Cdd:PRK03584 410 --DVWLASISGGTDIC---SCFVG--GNPLLPVYrgeiqcrglGMAVEAWDEDGRPVVGEV-GELVCTKPFPSMPLGFWN 481
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 478 DPD--RFIEAYFSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQ 555
Cdd:PRK03584 482 DPDgsRYRDAYFDTFPGVWRHGDWIEITEHGGVVIYGRSDATLNRGGVRIGTAEIYRQVEALPEVLDSLVIGQEWPDGDV 561
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 556 GICTFVTLQAGVPESEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIM----RRILR----KIAANeLDSLGDT 627
Cdd:PRK03584 562 RMPLFVVLAEGVTLDDALRARIRTTIRTNLSPRHVPDKIIAVPDIPRTLSGKKVelpvKKLLHgrpvKKAVN-RDALANP 640
|
....*
gi 446925704 628 STLAE 632
Cdd:PRK03584 641 EALDW 645
|
|
| MACS_like_2 |
cd05973 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
107-615 |
2.58e-86 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341277 [Multi-domain] Cd Length: 437 Bit Score: 276.71 E-value: 2.58e-86
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 107 SYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITaDS 186
Cdd:cd05973 2 TFGELRALSARFANALQELGVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQPLFTAFGPKAIEHRLRTSGARLVVT-DA 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 187 SLRAgKLlplkenvdlalalpgtecvenvivvyrnanpiemkpgrdlwyhliimevdancppepmkAEDPLFILYTSGST 266
Cdd:cd05973 81 ANRH-KL-----------------------------------------------------------DSDPFVMMFTSGTT 100
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 267 GKPKGVLHTTGGYLVYVAsTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFEGVPQYPTWARlghVVDK 346
Cdd:cd05973 101 GLPKGVPVPLRALAAFGA-YLRDAVDLRPEDSFWNAADPGWAYGLYYAITGPLALGHPTILLEGGFSVESTWR---VIER 176
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 347 HKVSILYTAPTAIRAMMREGDSyVRESNRSSLRLLGSVGEPINPEAWNWYYNVVGegrCPIVDTWWQTETGGILIAPLPG 426
Cdd:cd05973 177 LGVTNLAGSPTAYRLLMAAGAE-VPARPKGRLRRVSSAGEPLTPEVIRWFDAALG---VPIHDHYGQTELGMVLANHHAL 252
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 427 ATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDSWPGQM--RTIWGDPDRFIEAYFstfkntYFTGDGARRDE 504
Cdd:cd05973 253 EHPVHAGSAGRAMPGWRVAVLDDDGDELGPGEPGRLAIDIANSPLMwfRGYQLPDTPAIDGGY------YLTGDTVEFDP 326
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 505 DGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEELRKELISWVRKV 584
Cdd:cd05973 327 DGSFSFIGRADDVITMSGYRIGPFDVESALIEHPAVAEAAVIGVPDPERTEVVKAFVVLRGGHEGTPALADELQLHVKKR 406
|
490 500 510
....*....|....*....|....*....|.
gi 446925704 585 LGPVASPDALHWAPALPKTRSGKIMRRILRK 615
Cdd:cd05973 407 LSAHAYPRTIHFVDELPKTPSGKIQRFLLRR 437
|
|
| MACS_like_3 |
cd05971 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
104-614 |
6.05e-81 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341275 [Multi-domain] Cd Length: 439 Bit Score: 262.75 E-value: 6.05e-81
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 104 KIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVIT 183
Cdd:cd05971 5 EKVTFKELKTASNRFANVLKEIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASALVT 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 184 ADSSlragkllplkenvdlalalpgtecvenvivvyrnanpiemkpgrdlwyhliimevdancppepmkaeDPLFILYTS 263
Cdd:cd05971 85 DGSD-------------------------------------------------------------------DPALIIYTS 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 264 GSTGKPKGVLHTTG---GYLVYVASTFkEVFDlKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFEGVPQYPtwARL 340
Cdd:cd05971 98 GTTGPPKGALHAHRvllGHLPGVQFPF-NLFP-RDGDLYWTPADWAWIGGLLDVLLPSLYFGVPVLAHRMTKFDP--KAA 173
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 341 GHVVDKHKVSILYTAPTAIRAMMREGDSyvRESNRSSLRLLGSVGEPINPEAWNWYYNVVGEgrcPIVDTWWQTEtGGIL 420
Cdd:cd05971 174 LDLMSRYGVTTAFLPPTALKMMRQQGEQ--LKHAQVKLRAIATGGESLGEELLGWAREQFGV---EVNEFYGQTE-CNLV 247
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 421 IAPLPGATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDSWPGQMRTIWGDPdrfiEAYFSTFKNTYF-TGDG 499
Cdd:cd05971 248 IGNCSALFPIKPGSMGKPIPGHRVAIVDDNGTPLPPGEVGEIAVELPDPVAFLGYWNNP----SATEKKMAGDWLlTGDL 323
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 500 ARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEELRKELIS 579
Cdd:cd05971 324 GRKDSDGYFWYVGRDDDVITSSGYRIGPAEIEECLLKHPAVLMAAVVGIPDPIRGEIVKAFVVLNPGETPSDALAREIQE 403
|
490 500 510
....*....|....*....|....*....|....*
gi 446925704 580 WVRKVLGPVASPDALHWAPALPKTRSGKIMRRILR 614
Cdd:cd05971 404 LVKTRLAAHEYPREIEFVNELPRTATGKIRRRELR 438
|
|
| PTZ00237 |
PTZ00237 |
acetyl-CoA synthetase; Provisional |
21-615 |
9.37e-81 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 240325 [Multi-domain] Cd Length: 647 Bit Score: 268.53 E-value: 9.37e-81
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 21 DYFKRYQHSIENPDEFWAEQAK-IVDWIKPFTQVkntsFDKDNFKIEWFADGELNVSANCLDRHLKeHPHK---PAIIWE 96
Cdd:PTZ00237 9 DYENDSNYANSNPESFWDEVAKkYVHWDKMYDKV----YSGDEIYPDWFKGGELNTCYNVLDIHVK-NPLKrdqDALIYE 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 97 GDHPSRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDS 176
Cdd:PTZ00237 84 CPYLKKTIKLTYYQLYEKVCEFSRVLLNLNISKNDNVLIYMANTLEPLIAMLSCARIGATHCVLFDGYSVKSLIDRIETI 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 177 QAKLVITADSSLRAGKLLP----LKENVDLALALPgtecvENVIVVYRN----------ANPIEMKPGRDLWYHLIIMEV 242
Cdd:PTZ00237 164 TPKLIITTNYGILNDEIITftpnLKEAIELSTFKP-----SNVITLFRNditsesdlkkIETIPTIPNTLSWYDEIKKIK 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 243 DANCPP----EPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLiYGP 318
Cdd:PTZ00237 239 ENNQSPfyeyVPVESSHPLYILYTSGTTGNSKAVVRSNGPHLVGLKYYWRSIIEKDIPTVVFSHSSIGWVSFHGFL-YGS 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 319 LANGTTTLMFEG--VPQYPTWARLGHVVDKHKVSILYTAPTAIRAMMR---EGDSYVRESNRSSLRLLGSVGEPINPEAW 393
Cdd:PTZ00237 318 LSLGNTFVMFEGgiIKNKHIEDDLWNTIEKHKVTHTLTLPKTIRYLIKtdpEATIIRSKYDLSNLKEIWCGGEVIEESIP 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 394 NWYYNVVGeGRCPIVdtWWQTETGgilIAPLPGATAL-KPGSAT-RPLFGIQPAIVDGEGNELEGAAEGNLVIKDSWPGQ 471
Cdd:PTZ00237 398 EYIENKLK-IKSSRG--YGQTEIG---ITYLYCYGHInIPYNATgVPSIFIKPSILSEDGKELNVNEIGEVAFKLPMPPS 471
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 472 MRTIWGDPDRFIEAYFSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPH- 550
Cdd:PTZ00237 472 FATTFYKNDEKFKQLFSKFPGYYNSGDLGFKDENGYYTIVSRSDDQIKISGNKVQLNTIETSILKHPLVLECCSIGIYDp 551
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446925704 551 DIKGQGICTFVTLQAGVPES---EELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRK 615
Cdd:PTZ00237 552 DCYNVPIGLLVLKQDQSNQSidlNKLKNEINNIITQDIESLAVLRKIIIVNQLPKTKTGKIPRQIISK 619
|
|
| MACS_AAE_MA_like |
cd05970 |
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ... |
61-614 |
1.08e-80 |
|
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.
Pssm-ID: 341274 [Multi-domain] Cd Length: 537 Bit Score: 265.13 E-value: 1.08e-80
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 61 DNFKIEwfADGELNVSANCLDRHLKEHPHKPAIIWEGDHPSRhKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMV 140
Cdd:cd05970 6 NNFSIN--VPENFNFAYDVVDAMAKEYPDKLALVWCDDAGEE-RIFTFAELADYSDKTANFFKAMGIGKGDTVMLTLKRR 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 141 TEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITADsslraGKLLPlkENVDLALAlpgtECVENVIVVYR 220
Cdd:cd05970 83 YEFWYSLLALHKLGAIAIPATHQLTAKDIVYRIESADIKMIVAIA-----EDNIP--EEIEKAAP----ECPSKPKLVWV 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 221 NAnpiemkPGRDLW--YHLIIMEVDANCPP----EPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVAsTFKEVFDLK 294
Cdd:cd05970 152 GD------PVPEGWidFRKLIKNASPDFERptanSYPCGEDILLVYFSSGTTGMPKMVEHDFTYPLGHIV-TAKYWQNVR 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 295 QDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFEGVPQYPtwARLGHVVDKHKVSILYTAPTAIRAMMREGDSyvrESN 374
Cdd:cd05970 225 EGGLHLTVADTGWGKAVWGKIYGQWIAGAAVFVYDYDKFDP--KALLEKLSKYGVTTFCAPPTIYRFLIREDLS---RYD 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 375 RSSLRLLGSVGEPINPEAWNWYYNVVGegrCPIVDTWWQTETGgILIAPLPGATAlKPGSATRPLFGIQPAIVDGEGNEL 454
Cdd:cd05970 300 LSSLRYCTTAGEALNPEVFNTFKEKTG---IKLMEGFGQTETT-LTIATFPWMEP-KPGSMGKPAPGYEIDLIDREGRSC 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 455 EGAAEGNLVIK--DSWP-GQMRTIWGDPDRFIEAYFSTFkntYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIE 531
Cdd:cd05970 375 EAGEEGEIVIRtsKGKPvGLFGGYYKDAEKTAEVWHDGY---YHTGDAAWMDEDGYLWFVGRTDDLIKSSGYRIGPFEVE 451
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 532 SALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRR 611
Cdd:cd05970 452 SALIQHPAVLECAVTGVPDPIRGQVVKATIVLAKGYEPSEELKKELQDHVKKVTAPYKYPRIVEFVDELPKTISGKIRRV 531
|
...
gi 446925704 612 ILR 614
Cdd:cd05970 532 EIR 534
|
|
| BCL_4HBCL |
cd05959 |
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ... |
77-614 |
1.34e-80 |
|
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.
Pssm-ID: 341269 [Multi-domain] Cd Length: 508 Bit Score: 264.23 E-value: 1.34e-80
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 77 ANCLDRHLKE-HPHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGA 155
Cdd:cd05959 6 ATLVDLNLNEgRGDKTAFIDDAGS------LTYAELEAEARRVAGALRALGVKREERVLLIMLDTVDFPTAFLGAIRAGI 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 156 VHCVVFGGFSPDSLASRIEDSQAKLVITAdsslraGKLLPLkenvdLALALPGTECVENVIVVyrnANPIEMKPGRDLWY 235
Cdd:cd05959 80 VPVPVNTLLTPDDYAYYLEDSRARVVVVS------GELAPV-----LAAALTKSEHTLVVLIV---SGGAGPEAGALLLA 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 236 HLIIMEVDAnCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGyLVYVASTF-KEVFDLKQDDVYWCTADVGWITGHSYL 314
Cdd:cd05959 146 ELVAAEAEQ-LKPAATHADDPAFWLYSSGSTGRPKGVVHLHAD-IYWTAELYaRNVLGIREDDVCFSAAKLFFAYGLGNS 223
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 315 IYGPLANGTTTLMFegvPQYPTWARLGHVVDKHKVSILYTAPTAIRAMMREGDSyvRESNRSSLRLLGSVGEPINPEAWN 394
Cdd:cd05959 224 LTFPLSVGATTVLM---PERPTPAAVFKRIRRYRPTVFFGVPTLYAAMLAAPNL--PSRDLSSLRLCVSAGEALPAEVGE 298
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 395 WYYNVVGegrCPIVDTWWQTETGGILIAPLPGAtaLKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDswPGQMRT 474
Cdd:cd05959 299 RWKARFG---LDILDGIGSTEMLHIFLSNRPGR--VRYGTTGKPVPGYEVELRDEDGGDVADGEPGELYVRG--PSSATM 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 475 IWGDPDrfieAYFSTFKNTYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIK 553
Cdd:cd05959 372 YWNNRD----KTRDTFQGEWTrTGDKYVRDDDGFYTYAGRADDMLKVSGIWVSPFEVESALVQHPAVLEAAVVGVEDEDG 447
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 446925704 554 GQGICTFVTLQAGVPESEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILR 614
Cdd:cd05959 448 LTKPKAFVVLRPGYEDSEALEEELKEFVKDRLAPYKYPRWIVFVDELPKTATGKIQRFKLR 508
|
|
| FACL_FadD13-like |
cd17631 |
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ... |
80-610 |
2.81e-76 |
|
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.
Pssm-ID: 341286 [Multi-domain] Cd Length: 435 Bit Score: 250.22 E-value: 2.81e-76
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIWEGdhpsrhKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCV 159
Cdd:cd17631 1 LRRRARRHPDRTALVFGG------RSLTYAELDERVNRLAHALRALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVP 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFGGFSPDSLASRIEDSQAKLVItadsslragkllplkenvdlalalpgtecvenvivvyrnanpiemkpgrdlwyhlii 239
Cdd:cd17631 75 LNFRLTPPEVAYILADSGAKVLF--------------------------------------------------------- 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 240 mevdancppepmkaEDPLFILYTSGSTGKPKGVLHTTGGYLVYVASTFKEvFDLKQDDVYWCTADVGWITGHSYLIYGPL 319
Cdd:cd17631 98 --------------DDLALLMYTSGTTGRPKGAMLTHRNLLWNAVNALAA-LDLGPDDVLLVVAPLFHIGGLGVFTLPTL 162
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 320 ANGTTTLMFEGvpqyPTWARLGHVVDKHKVSILYTAPTAIRAMMREGDSyvRESNRSSLRLLGSVGEPINP---EAWnWY 396
Cdd:cd17631 163 LRGGTVVILRK----FDPETVLDLIERHRVTSFFLVPTMIQALLQHPRF--ATTDLSSLRAVIYGGAPMPErllRAL-QA 235
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 397 YNVVgegrcpIVDTWWQTETGgiliaplPGATAL-------KPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDswP 469
Cdd:cd17631 236 RGVK------FVQGYGMTETS-------PGVTFLspedhrrKLGSAGRPVFFVEVRIVDPDGREVPPGEVGEIVVRG--P 300
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 470 GQMRTIWGDPdrfiEAYFSTFKNTYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGM 548
Cdd:cd17631 301 HVMAGYWNRP----EATAAAFRDGWFhTGDLGRLDEDGYLYIVDRKKDMIISGGENVYPAEVEDVLYEHPAVAEVAVIGV 376
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 446925704 549 PHDIKGQGICTFVTLQAGVPESEElrkELISWVRKVLGPVASPDALHWAPALPKTRSGKIMR 610
Cdd:cd17631 377 PDEKWGEAVVAVVVPRPGAELDED---ELIAHCRERLARYKIPKSVEFVDALPRNATGKILK 435
|
|
| BCL_like |
cd05919 |
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ... |
106-614 |
4.95e-72 |
|
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.
Pssm-ID: 341243 [Multi-domain] Cd Length: 436 Bit Score: 239.29 E-value: 4.95e-72
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 106 VSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITAd 185
Cdd:cd05919 11 VTYGQLHDGANRLGSALRNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIARDCEARLVVTS- 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 186 sslragkllplkenvdlalalpgtecvenvivvyrnanpiemkpgrdlwyhliimevdancppepmkAEDPLFILYTSGS 265
Cdd:cd05919 90 -------------------------------------------------------------------ADDIAYLLYSSGT 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 266 TGKPKGVLHTTGGYLVYVASTFKEVFDLKQDDVYWCTADV--GWITGHSylIYGPLANGTTTLMFegvPQYPTWARLGHV 343
Cdd:cd05919 103 TGPPKGVMHAHRDPLLFADAMAREALGLTPGDRVFSSAKMffGYGLGNS--LWFPLAVGASAVLN---PGWPTAERVLAT 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 344 VDKHKVSILYTAPTAIRAMMREGDsYVRESNRSsLRLLGSVGEPINPEAWNWYYNVVGegrCPIVDTWWQTETGGILIAP 423
Cdd:cd05919 178 LARFRPTVLYGVPTFYANLLDSCA-GSPDALRS-LRLCVSAGEALPRGLGERWMEHFG---GPILDGIGATEVGHIFLSN 252
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 424 LPGAtaLKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDswPGQMRTIWGDPD----RFIEAYfstfkntYFTGDG 499
Cdd:cd05919 253 RPGA--WRLGSTGRPVPGYEIRLVDEEGHTIPPGEEGDLLVRG--PSAAVGYWNNPEksraTFNGGW-------YRTGDK 321
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 500 ARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEELRKELIS 579
Cdd:cd05919 322 FCRDADGWYTHAGRADDMLKVGGQWVSPVEVESLIIQHPAVAEAAVVAVPESTGLSRLTAFVVLKSPAAPQESLARDIHR 401
|
490 500 510
....*....|....*....|....*....|....*
gi 446925704 580 WVRKVLGPVASPDALHWAPALPKTRSGKIMRRILR 614
Cdd:cd05919 402 HLLERLSAHKVPRRIAFVDELPRTATGKLQRFKLR 436
|
|
| PRK06187 |
PRK06187 |
long-chain-fatty-acid--CoA ligase; Validated |
70-614 |
5.92e-71 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235730 [Multi-domain] Cd Length: 521 Bit Score: 238.93 E-value: 5.92e-71
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 70 DGELNVsANCLDRHLKEHPHKPAIIWEGdhpsrhKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLA 149
Cdd:PRK06187 3 DYPLTI-GRILRHGARKHPDKEAVYFDG------RRTTYAELDERVNRLANALRALGVKKGDRVAVFDWNSHEYLEAYFA 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 150 CARIGAV-HCV-VFggFSPDSLASRIEDSQAKLVItADSSLragklLPLkenvdLALALPGTECVENVIVVyrnaNPIEM 227
Cdd:PRK06187 76 VPKIGAVlHPInIR--LKPEEIAYILNDAEDRVVL-VDSEF-----VPL-----LAAILPQLPTVRTVIVE----GDGPA 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 228 KPGRDLWYHLiiMEVDANCPPEPMKAE----DPLFILYTSGSTGKPKGVLHTTggylvyvASTF------KEVFDLKQDD 297
Cdd:PRK06187 139 APLAPEVGEY--EELLAAASDTFDFPDidenDAAAMLYTSGTTGHPKGVVLSH-------RNLFlhslavCAWLKLSRDD 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 298 VYwctadvgwitghsyLIYGPLAN-GTTTL----MFEGVPQ-----YPTwARLGHVVDKHKVSILYTAPTAIRAMMREGD 367
Cdd:PRK06187 210 VY--------------LVIVPMFHvHAWGLpylaLMAGAKQviprrFDP-ENLLDLIETERVTFFFAVPTIWQMLLKAPR 274
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 368 SYVRESnrSSLRLLGSVGEPINPEAWNWYYNVVGegrCPIVDTWWQTETGGIL-IAPLP---GATALKPGSATRPLFGIQ 443
Cdd:PRK06187 275 AYFVDF--SSLRLVIYGGAALPPALLREFKEKFG---IDLVQGYGMTETSPVVsVLPPEdqlPGQWTKRRSAGRPLPGVE 349
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 444 PAIVDGEGNELE--GAAEGNLVIKDSWpgQMRTIWGDPdrfiEAYFSTFKNT-YFTGDGARRDEDGYYWITGRVDDVLNV 520
Cdd:PRK06187 350 ARIVDDDGDELPpdGGEVGEIIVRGPW--LMQGYWNRP----EATAETIDGGwLHTGDVGYIDEDGYLYITDRIKDVIIS 423
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 521 SGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEelrKELISWVRKVLGPVASPDALHWAPAL 600
Cdd:PRK06187 424 GGENIYPRELEDALYGHPAVAEVAVIGVPDEKWGERPVAVVVLKPGATLDA---KELRAFLRGRLAKFKLPKRIAFVDEL 500
|
570
....*....|....
gi 446925704 601 PKTRSGKIMRRILR 614
Cdd:PRK06187 501 PRTSVGKILKRVLR 514
|
|
| MACS_euk |
cd05928 |
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ... |
86-615 |
4.38e-69 |
|
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.
Pssm-ID: 341251 [Multi-domain] Cd Length: 530 Bit Score: 234.28 E-value: 4.38e-69
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 86 EHPHKPAIIWEGDHPSRHKIvSYKELHDEVCRFANVLKKY-GIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGF 164
Cdd:cd05928 23 KRPPNPALWWVNGKGDEVKW-SFRELGSLSRKAANVLSGAcGLQRGDRVAVILPRVPEWWLVNVACIRTGLVFIPGTIQL 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 165 SPDSLASRIEDSQAKLVITADSslragkLLPLKENVdlALALPgteCVENVIVVYRNAnpiemkpgRDLWYHL--IIMEV 242
Cdd:cd05928 102 TAKDILYRLQASKAKCIVTSDE------LAPEVDSV--ASECP---SLKTKLLVSEKS--------RDGWLNFkeLLNEA 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 243 DANCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANG 322
Cdd:cd05928 163 STEHHCVETGSQEPMAIYFTSGTTGSPKMAEHSHSSLGLGLKVNGRYWLDLTASDIMWNTSDTGWIKSAWSSLFEPWIQG 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 323 TTTLMFEgVPQYPTWARLgHVVDKHKVSILYTAPTAIRAMMREGDSYVResnRSSLRLLGSVGEPINPEAWNWYYNVVGe 402
Cdd:cd05928 243 ACVFVHH-LPRFDPLVIL-KTLSSYPITTFCGAPTVYRMLVQQDLSSYK---FPSLQHCVTGGEPLNPEVLEKWKAQTG- 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 403 grCPIVDTWWQTETGgiLIAPLPGATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVI--KDSWPGQMRTIW-GDP 479
Cdd:cd05928 317 --LDIYEGYGQTETG--LICANFKGMKIKPGSMGKASPPYDVQIIDDNGNVLPPGTEGDIGIrvKPIRPFGLFSGYvDNP 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 480 DRFIEAYFSTFkntYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICT 559
Cdd:cd05928 393 EKTAATIRGDF---YLTGDRGIMDEDGYFWFMGRADDVINSSGYRIGPFEVESALIEHPAVVESAVVSSPDPIRGEVVKA 469
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 446925704 560 FVTLQAGVPES--EELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRK 615
Cdd:cd05928 470 FVVLAPQFLSHdpEQLTKELQQHVKSVTAPYKYPRKVEFVQELPKTVTGKIQRNELRD 527
|
|
| FC-FACS_FadD_like |
cd05936 |
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ... |
80-614 |
4.81e-69 |
|
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341259 [Multi-domain] Cd Length: 468 Bit Score: 232.07 E-value: 4.81e-69
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIWEGdhpsrhKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGA--VH 157
Cdd:cd05936 5 LEEAARRFPDKTALIFMG------RKLTYRELDALAEAFAAGLQNLGVQPGDRVALMLPNCPQFPIAYFGALKAGAvvVP 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 158 CVVFggFSPDSLASRIEDSQAKLVITAdsslragklLPLKENVDLALALPGtecvenvivvyrnanpiemKPGRDlwyhl 237
Cdd:cd05936 79 LNPL--YTPRELEHILNDSGAKALIVA---------VSFTDLLAAGAPLGE-------------------RVALT----- 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 238 iimevdancppepmkAEDPLFILYTSGSTGKPKGVLHTTGGyLVYVASTFKEVF--DLKQDDVYWCTADVgwitghsYLI 315
Cdd:cd05936 124 ---------------PEDVAVLQYTSGTTGVPKGAMLTHRN-LVANALQIKAWLedLLEGDDVVLAALPL-------FHV 180
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 316 YGPLANGTTTLMFEG----VPQyPTWARLGHVVDKHKVSILYTAPTAIRAMMREGDsyVRESNRSSLRLLGSVGEPINPE 391
Cdd:cd05936 181 FGLTVALLLPLALGAtivlIPR-FRPIGVLKEIRKHRVTIFPGVPTMYIALLNAPE--FKKRDFSSLRLCISGGAPLPVE 257
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 392 AWNWYYNVVGegrCPIVDTWWQTETGGILIA-PLPGATalKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDswPG 470
Cdd:cd05936 258 VAERFEELTG---VPIVEGYGLTETSPVVAVnPLDGPR--KPGSIGIPLPGTEVKIVDDDGEELPPGEVGELWVRG--PQ 330
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 471 QMRTIWGDPdrfiEAYFSTFKNTYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMP 549
Cdd:cd05936 331 VMKGYWNRP----EETAEAFVDGWLrTGDIGYMDEDGYFFIVDRKKDMIIVGGFNVYPREVEEVLYEHPAVAEAAVVGVP 406
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 446925704 550 HDIKGQGICTFVTLQAGVPESEElrkELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILR 614
Cdd:cd05936 407 DPYSGEAVKAFVVLKEGASLTEE---EIIAFCREQLAGYKVPRQVEFRDELPKSAVGKILRRELR 468
|
|
| ac_ac_CoA_syn |
TIGR01217 |
acetoacetyl-CoA synthase; This enzyme catalyzes the first step of the mevalonate pathway of ... |
19-618 |
6.13e-65 |
|
acetoacetyl-CoA synthase; This enzyme catalyzes the first step of the mevalonate pathway of IPP biosynthesis. Most bacteria do not use this pathway, but rather the deoxyxylulose pathway. [Central intermediary metabolism, Other]
Pssm-ID: 273507 [Multi-domain] Cd Length: 652 Bit Score: 225.92 E-value: 6.13e-65
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 19 EADYFKRYQHSIENPDEFWAEQAKIVDWI--KPFTQVKNTSFDKDNfkiEWFADGELNVSANCLdRHLKEhphKPAIIWE 96
Cdd:TIGR01217 34 EGGYDALHRWSVDELDTFWKAVWEWFDVRfsTPCARVVDDRTMPGA---QWFPGARLNYAENLL-RAAGT---EPALLYV 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 97 GDHPSRHKiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDS 176
Cdd:TIGR01217 107 DETHEPAP-VTWAELRRQVASLAAALRALGVRPGDRVSGYLPNIPQAVVAMLATASVGAIWSSCSPDFGARGVLDRFQQI 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 177 QAKLVITADSSLRAGKLLPLKENV-DLALALPGTECVENVIVVYRNANPIEMKPGRDLWYHLIIMEVDANCPPEPMKAED 255
Cdd:TIGR01217 186 EPKLLFTVDGYRYNGKEHDRRDKVaEVRKELPTLRAVVHIPYLGPRETEAPKIDGALDLEDFTAAAQAAELVFEQLPFDH 265
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 256 PLFILYTSGSTGKPKGVLHTTGGYLVYVASTFKEVFDLKQDDVYWCTADVGWITgHSYLIYGpLANGTTTLMFEGVPQYP 335
Cdd:TIGR01217 266 PLWILFSSGTTGLPKCIVHSAGGTLVQHLKEHGLHCDLGPGDRLFYYTTTGWMM-WNWLVSG-LATGATLVLYDGSPGFP 343
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 336 TWARLGHVVDKHKVSILYTAPTAIRAMMREGDSYVRESNRSSLRLLGSVGEPINPEAWNWYYnvvgegRCPIVDTWWQTE 415
Cdd:TIGR01217 344 ATNVLWDIAERTGATLFGTSAKYVMACRKAGVHPARTHDLSALQCVASTGSPLPPDGFRWVY------DEIKADVWLASI 417
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 416 TGGI-LIAPLPGATALKP---GSATRPLFGIQPAIVDGEGNELEGAAeGNLVIKDSWPGQMRTIWGDPD--RFIEAYFST 489
Cdd:TIGR01217 418 SGGTdICSCFAGANPTLPvhiGEIQAPGLGTAVQSWDPEGKPVTGEV-GELVCTNPMPSMPIRFWNDPDgsKYRDAYFDT 496
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 490 FKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPE 569
Cdd:TIGR01217 497 YPGVWRHGDWITLTPRGGIVIHGRSDSTLNPQGVRMGSAEIYNAVERLDEVRESLCIGQEQPDGGYRVVLFVHLAPGATL 576
|
570 580 590 600
....*....|....*....|....*....|....*....|....*....
gi 446925704 570 SEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKIAA 618
Cdd:TIGR01217 577 DDALLDRIKRTIRAGLSPRHVPDEIIEVPGIPHTLTGKRVEVAVKRVLQ 625
|
|
| PRK08316 |
PRK08316 |
acyl-CoA synthetase; Validated |
87-618 |
3.37e-62 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181381 [Multi-domain] Cd Length: 523 Bit Score: 215.57 E-value: 3.37e-62
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 87 HPHKPAIIWegdhpsRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSP 166
Cdd:PRK08316 24 YPDKTALVF------GDRSWTYAELDAAVNRVAAALLDLGLKKGDRVAALGHNSDAYALLWLACARAGAVHVPVNFMLTG 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 167 DSLASRIEDSQAKLVITAD---SSLRAGKLLPLKENVDLALALPGTEcvenvivvyrnanpiemKPGRDLWYHLIIMEVD 243
Cdd:PRK08316 98 EELAYILDHSGARAFLVDPalaPTAEAALALLPVDTLILSLVLGGRE-----------------APGGWLDFADWAEAGS 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 244 ANCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLV-YVASTFKevFDLKQDDV-------YWCTADvgwitgHSYLi 315
Cdd:PRK08316 161 VAEPDVELADDDLAQILYTSGTESLPKGAMLTHRALIAeYVSCIVA--GDMSADDIplhalplYHCAQL------DVFL- 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 316 yGP-LANGTTTLMFEGvpqyPTWARLGHVVDKHKVSILYTAPTAIRAMMREGDsyVRESNRSSLRLlGSVGEPINPEA-- 392
Cdd:PRK08316 232 -GPyLYVGATNVILDA----PDPELILRTIEAERITSFFAPPTVWISLLRHPD--FDTRDLSSLRK-GYYGASIMPVEvl 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 393 ------------WNWYynvvgeGrcpivdtwwQTEtggilIAPLpgATAL-------KPGSATRPLFGIQPAIVDGEGNE 453
Cdd:PRK08316 304 kelrerlpglrfYNCY------G---------QTE-----IAPL--ATVLgpeehlrRPGSAGRPVLNVETRVVDDDGND 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 454 LEGAAEGNLVIKDswPGQMRTIWGDPDRFIEAyfstFKNTYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIES 532
Cdd:PRK08316 362 VAPGEVGEIVHRS--PQLMLGYWDDPEKTAEA----FRGGWFhSGDLGVMDEEGYITVVDRKKDMIKTGGENVASREVEE 435
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 533 ALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEElrkELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRI 612
Cdd:PRK08316 436 ALYTHPAVAEVAVIGLPDPKWIEAVTAVVVPKAGATVTED---ELIAHCRARLAGFKVPKRVIFVDELPRNPSGKILKRE 512
|
....*.
gi 446925704 613 LRKIAA 618
Cdd:PRK08316 513 LRERYA 518
|
|
| FACL_DitJ_like |
cd05934 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
107-614 |
1.64e-60 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.
Pssm-ID: 341257 [Multi-domain] Cd Length: 422 Bit Score: 207.91 E-value: 1.64e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 107 SYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITads 186
Cdd:cd05934 5 TYAELLRESARIAAALAALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLVVV--- 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 187 slragkllplkenvdlalalpgtecvenvivvyrnanpiemkpgrdlwyhliimevdancppepmkaeDPLFILYTSGST 266
Cdd:cd05934 82 --------------------------------------------------------------------DPASILYTSGTT 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 267 GKPKGVLhTTGGYLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMfegVPQYPT---WArlghV 343
Cdd:cd05934 94 GPPKGVV-ITHANLTFAGYYSARRFGLGEDDVYLTVLPLFHINAQAVSVLAALSVGATLVL---LPRFSAsrfWS----D 165
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 344 VDKHKVSILYTAPTAIRAMMREGDSYVRESNRssLRLLGsvGEPINPEAWNWYYNVVGegrCPIVDTWWQTETGGILIAP 423
Cdd:cd05934 166 VRRYGATVTNYLGAMLSYLLAQPPSPDDRAHR--LRAAY--GAPNPPELHEEFEERFG---VRLLEGYGMTETIVGVIGP 238
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 424 LPGATalKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDSWP-GQMRTIWGDPDRFIEAyfstFKNTYF-TGDGAR 501
Cdd:cd05934 239 RDEPR--RPGSIGRPAPGYEVRIVDDDGQELPAGEPGELVIRGLRGwGFFKGYYNMPEATAEA----MRNGWFhTGDLGY 312
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 502 RDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEElrkELISWV 581
Cdd:cd05934 313 RDADGFFYFVDRKKDMIRRRGENISSAEVERAILRHPAVREAAVVAVPDEVGEDEVKAVVVLRPGETLDPE---ELFAFC 389
|
490 500 510
....*....|....*....|....*....|...
gi 446925704 582 RKVLGPVASPDALHWAPALPKTRSGKIMRRILR 614
Cdd:cd05934 390 EGQLAYFKVPRYIRFVDDLPKTPTEKVAKAQLR 422
|
|
| MACS_like_1 |
cd05974 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
106-617 |
1.87e-60 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341278 [Multi-domain] Cd Length: 432 Bit Score: 208.19 E-value: 1.87e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 106 VSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITAD 185
Cdd:cd05974 1 VSFAEMSARSSRVANFLRSIGVGRGDRILLMLGNVVELWEAMLAAMKLGAVVIPATTLLTPDDLRDRVDRGGAVYAAVDE 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 186 SSlragkllplkenvdlalalpgtecvenvivvyrnanpiemkpgrdlwyhliimevdancppepmKAEDPLFILYTSGS 265
Cdd:cd05974 81 NT----------------------------------------------------------------HADDPMLLYFTSGT 96
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 266 TGKPKGVLHTTGGYLVYVASTFKEVfDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFegvpQYPTW--ARLGHV 343
Cdd:cd05974 97 TSKPKLVEHTHRSYPVGHLSTMYWI-GLKPGDVHWNISSPGWAKHAWSCFFAPWNAGATVFLF----NYARFdaKRVLAA 171
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 344 VDKHKVSILYTAPTAIRAMMREGDSyvreSNRSSLRLLGSVGEPINPE-------AWNwyynvvgegrCPIVDTWWQTET 416
Cdd:cd05974 172 LVRYGVTTLCAPPTVWRMLIQQDLA----SFDVKLREVVGAGEPLNPEvieqvrrAWG----------LTIRDGYGQTET 237
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 417 GgILIAPLPGATaLKPGSATRPLFGIQPAIVDGEGNElegAAEGN--LVIKDSWP-GQMRTIWGDPDRFIEAYFSTFknt 493
Cdd:cd05974 238 T-ALVGNSPGQP-VKAGSMGRPLPGYRVALLDPDGAP---ATEGEvaLDLGDTRPvGLMKGYAGDPDKTAHAMRGGY--- 309
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 494 YFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEEL 573
Cdd:cd05974 310 YRTGDIAMRDEDGYLTYVGRADDVFKSSDYRISPFELESVLIEHPAVAEAAVVPSPDPVRLSVPKAFIVLRAGYEPSPET 389
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 446925704 574 RKELISWVRKVLGPVASPDALHWApALPKTRSGKIMRRILRKIA 617
Cdd:cd05974 390 ALEIFRFSRERLAPYKRIRRLEFA-ELPKTISGKIRRVELRRRE 432
|
|
| PRK07656 |
PRK07656 |
long-chain-fatty-acid--CoA ligase; Validated |
80-615 |
5.12e-58 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236072 [Multi-domain] Cd Length: 513 Bit Score: 203.98 E-value: 5.12e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCV 159
Cdd:PRK07656 11 LARAARRFGDKEAYVFGDQR------LTYAELNARVRRAAAALAALGIGKGDRVAIWAPNSPHWVIAALGALKAGAVVVP 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFGGFSPDSLASRIEDSQAKLVITADsslragKLLPLKENVDLALALpgtecVENVIVVyRNANPIEMKPGRDLWYHLII 239
Cdd:PRK07656 85 LNTRYTADEAAYILARGDAKALFVLG------LFLGVDYSATTRLPA-----LEHVVIC-ETEEDDPHTEKMKTFTDFLA 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 240 MEVDANCPPEpMKAEDPLFILYTSGSTGKPKGVLhTTGGYLVYVASTFKEVFDLKQDDVYWCTA--------DVGWITgh 311
Cdd:PRK07656 153 AGDPAERAPE-VDPDDVADILFTSGTTGRPKGAM-LTHRQLLSNAADWAEYLGLTEGDRYLAANpffhvfgyKAGVNA-- 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 312 syliygPLANGTTTLMfegVPQY-PtwARLGHVVDKHKVSILYTAPTAIRAMMREGDSyvRESNRSSLRLLGSVGEPINP 390
Cdd:PRK07656 229 ------PLMRGATILP---LPVFdP--DEVFRLIETERITVLPGPPTMYNSLLQHPDR--SAEDLSSLRLAVTGAASMPV 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 391 EAWNwyyNVVGEGRCPIVDTWWQ-TETGGIL-IAPLPGATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDsw 468
Cdd:PRK07656 296 ALLE---RFESELGVDIVLTGYGlSEASGVTtFNRLDDDRKTVAGTIGTAIAGVENKIVNELGEEVPVGEVGELLVRG-- 370
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 469 PGQMRTIWGDPDRFIEAYfsTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGM 548
Cdd:PRK07656 371 PNVMKGYYDDPEATAAAI--DADGWLHTGDLGRLDEEGYLYIVDRKKDMFIVGGFNVYPAEVEEVLYEHPAVAEAAVIGV 448
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446925704 549 PHDIKGQGICTFVTLQAGVPESEElrkELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRK 615
Cdd:PRK07656 449 PDERLGEVGKAYVVLKPGAELTEE---ELIAYCREHLAKYKVPRSIEFLDELPKNATGKVLKRALRE 512
|
|
| ABCL |
cd05958 |
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ... |
101-614 |
1.47e-56 |
|
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.
Pssm-ID: 341268 [Multi-domain] Cd Length: 439 Bit Score: 198.09 E-value: 1.47e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 101 SRHKIVSYKELHDEVCRFANVLK-KYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVhcvvfggfspdslasriedsqak 179
Cdd:cd05958 6 SPEREWTYRDLLALANRIANVLVgELGIVPGNRVLLRGSNSPELVACWFGIQKAGAI----------------------- 62
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 180 lVITADSSLRAGKLLPLKENVDLALALpgteCVENVIVVyrnanpiemkpgrdlwyhliimevdancppepmkaEDPLFI 259
Cdd:cd05958 63 -AVATMPLLRPKELAYILDKARITVAL----CAHALTAS-----------------------------------DDICIL 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 260 LYTSGSTGKPKGVLHTTGGYLVyVASTF-KEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFEG-VPQyptw 337
Cdd:cd05958 103 AFTSGTTGAPKATMHFHRDPLA-SADRYaVNVLRLREDDRFVGSPPLAFTFGLGGVLLFPFGVGASGVLLEEaTPD---- 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 338 aRLGHVVDKHKVSILYTAPTAIRAMMREGDSyvRESNRSSLRLLGSVGEPINPEAWNWYYNVVGegrCPIVDTWWQTETG 417
Cdd:cd05958 178 -LLLSAIARYKPTVLFTAPTAYRAMLAHPDA--AGPDLSSLRKCVSAGEALPAALHRAWKEATG---IPIIDGIGSTEMF 251
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 418 GILIAPLPGAtaLKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDSwpgqmrTI-WGDPDRFIEAYFSTFKNTyfT 496
Cdd:cd05958 252 HIFISARPGD--ARPGATGKPVPGYEAKVVDDEGNPVPDGTIGRLAVRGP------TGcRYLADKRQRTYVQGGWNI--T 321
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 497 GDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEELRKE 576
Cdd:cd05958 322 GDTYSRDPDGYFRHQGRSDDMIVSGGYNIAPPEVEDVLLQHPAVAECAVVGHPDESRGVVVKAFVVLRPGVIPGPVLARE 401
|
490 500 510
....*....|....*....|....*....|....*...
gi 446925704 577 LISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILR 614
Cdd:cd05958 402 LQDHAKAHIAPYKYPRAIEFVTELPRTATGKLQRFALR 439
|
|
| benz_CoA_lig |
TIGR02262 |
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ... |
107-615 |
2.09e-56 |
|
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ligase, 4-hydroxybenzoate-CoA ligase, 2-aminobenzoate-CoA ligase, etc. Members are related to fatty acid and acetate CoA ligases.
Pssm-ID: 274059 [Multi-domain] Cd Length: 505 Bit Score: 199.29 E-value: 2.09e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 107 SYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITAds 186
Cdd:TIGR02262 32 SYGELEAQVRRLAAALRRLGVKREERVLLLMLDGVDFPIAFLGAIRAGIVPVALNTLLTADDYAYMLEDSRARVVFVS-- 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 187 slraGKLLPLkenvdLALALPGTECVENVIVVYRNanpiemKPGRDLWYHLIIMEVDANcPPEPMKAEDPLFILYTSGST 266
Cdd:TIGR02262 110 ----GALLPV-----IKAALGKSPHLEHRVVVGRP------EAGEVQLAELLATESEQF-KPAATQADDPAFWLYSSGST 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 267 GKPKGVLHTTGGYLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFegvPQYPTWARLGHVVDK 346
Cdd:TIGR02262 174 GMPKGVVHTHSNPYWTAELYARNTLGIREDDVCFSAAKLFFAYGLGNALTFPMSVGATTVLM---GERPTPDAVFDRLRR 250
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 347 HKVSILYTAPTAIRAMMREGDSyvRESNRSSLRLLGSVGEPINPEAWNWYYNVVGegrCPIVDTWWQTETGGILIAPLPG 426
Cdd:TIGR02262 251 HQPTIFYGVPTLYAAMLADPNL--PSEDQVRLRLCTSAGEALPAEVGQRWQARFG---VDIVDGIGSTEMLHIFLSNLPG 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 427 AtaLKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIkdSWPGQMRTIWGDPDRfieaYFSTFKNTYF-TGDGARRDED 505
Cdd:TIGR02262 326 D--VRYGTSGKPVPGYRLRLVGDGGQDVADGEPGELLI--SGPSSATMYWNNRAK----SRDTFQGEWTrSGDKYVRNDD 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 506 GYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMP---HDIKGQGictFVTLQAGvpeSEELRKELISWVR 582
Cdd:TIGR02262 398 GSYTYAGRTDDMLKVSGIYVSPFEIESALIQHPAVLEAAVVGVAdedGLIKPKA---FVVLRPG---QTALETELKEHVK 471
|
490 500 510
....*....|....*....|....*....|...
gi 446925704 583 KVLGPVASPDALHWAPALPKTRSGKIMRRILRK 615
Cdd:TIGR02262 472 DRLAPYKYPRWIVFVDDLPKTATGKIQRFKLRE 504
|
|
| A_NRPS |
cd05930 |
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ... |
88-611 |
1.07e-55 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341253 [Multi-domain] Cd Length: 444 Bit Score: 195.82 E-value: 1.07e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 88 PHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVfggfSPD 167
Cdd:cd05930 1 PDAVAVVDGDQS------LTYAELDARANRLARYLRERGVGPGDLVAVLLERSLEMVVAILAVLKAGAAYVPL----DPS 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 168 SLASRI----EDSQAKLVITADSslragkllplkenvdlalalpgtecvenvivvyrnanpiemkpgrDLWYhliimevd 243
Cdd:cd05930 71 YPAERLayilEDSGAKLVLTDPD---------------------------------------------DLAY-------- 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 244 ancppepmkaedplfILYTSGSTGKPKGVLHTTGGYLVYVAStFKEVFDLKQDDVYWCTA----DVGWitghsYLIYGPL 319
Cdd:cd05930 98 ---------------VIYTSGSTGKPKGVMVEHRGLVNLLLW-MQEAYPLTPGDRVLQFTsfsfDVSV-----WEIFGAL 156
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 320 ANGTTTLMF-EGVPQYPtwARLGHVVDKHKVSILYTAPTAIRAMMREGDsyvrESNRSSLRLLGSVGEPINPEAWN-WY- 396
Cdd:cd05930 157 LAGATLVVLpEEVRKDP--EALADLLAEEGITVLHLTPSLLRLLLQELE----LAALPSLRLVLVGGEALPPDLVRrWRe 230
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 397 -------YNVVGEGRCPIVDTWWQTETGGILIAPLP-GatalkpgsatRPLFGIQPAIVDGEGNELEGAAEGNLVIkdSW 468
Cdd:cd05930 231 llpgarlVNLYGPTEATVDATYYRVPPDDEEDGRVPiG----------RPIPNTRVYVLDENLRPVPPGVPGELYI--GG 298
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 469 PGQMRTIWGDPD----RFIEAYFstFKNT--YFTGDGARRDEDG--YYwiTGRVDDVLNVSGHRLGTAEIESALVSHEAV 540
Cdd:cd05930 299 AGLARGYLNRPEltaeRFVPNPF--GPGErmYRTGDLVRWLPDGnlEF--LGRIDDQVKIRGYRIELGEIEAALLAHPGV 374
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 446925704 541 AEAAVVGMPHDIKGQGICTFVTL-QAGVPESEELRKELiswvRKVLGPVASPDALHWAPALPKTRSGKIMRR 611
Cdd:cd05930 375 REAAVVAREDGDGEKRLVAYVVPdEGGELDEEELRAHL----AERLPDYMVPSAFVVLDALPLTPNGKVDRK 442
|
|
| FACL_fum10p_like |
cd05926 |
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ... |
88-615 |
2.40e-55 |
|
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.
Pssm-ID: 341249 [Multi-domain] Cd Length: 493 Bit Score: 195.99 E-value: 2.40e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 88 PHKPAIIWEGDHPSrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPD 167
Cdd:cd05926 1 PDAPALVVPGSTPA----LTYADLAELVDDLARQLAALGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKA 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 168 SLASRIEDSQAKLVIT-ADSSLRAGKLLPLKENVDLALALPGTECVENVivvyrnanpiemkPGRDLWYHLiiMEVDANC 246
Cdd:cd05926 77 EFEFYLADLGSKLVLTpKGELGPASRAASKLGLAILELALDVGVLIRAP-------------SAESLSNLL--ADKKNAK 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 247 PPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLV---YVASTFKevfdLKQDDVYWCTADVGWITGhsyLIYG---PLA 320
Cdd:cd05926 142 SEGVPLPDDLALILHTSGTTGRPKGVPLTHRNLAAsatNITNTYK----LTPDDRTLVVMPLFHVHG---LVASllsTLA 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 321 NGTTTLMFEG---------VPQY-PTWarlghvvdkhkvsilYTA-PTAIRAM-MREGDSYvrESNRSSLRLLGSVGEPI 388
Cdd:cd05926 215 AGGSVVLPPRfsastfwpdVRDYnATW---------------YTAvPTIHQILlNRPEPNP--ESPPPKLRFIRSCSASL 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 389 NPEAWNWYYNVVGegrCPIVDTWWQTETGGILIA-PLPgATALKPGSATRPlFGIQPAIVDGEGNELEGAAEGNLVIKDs 467
Cdd:cd05926 278 PPAVLEALEATFG---APVLEAYGMTEAAHQMTSnPLP-PGPRKPGSVGKP-VGVEVRILDEDGEILPPGVVGEICLRG- 351
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 468 wPGQMRTIWGDPDRFIEayfSTFKNTYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVV 546
Cdd:cd05926 352 -PNVTRGYLNNPEANAE---AAFKDGWFrTGDLGYLDADGYLFLTGRIKELINRGGEKISPLEVDGVLLSHPAVLEAVAF 427
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 446925704 547 GMPHDIKGQGICTFVTLQAGVPESEElrkELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRK 615
Cdd:cd05926 428 GVPDEKYGEEVAAAVVLREGASVTEE---ELRAFCRKHLAAFKVPKKVYFVDELPKTATGKIQRRKVAE 493
|
|
| PLN03052 |
PLN03052 |
acetate--CoA ligase; Provisional |
26-621 |
2.30e-53 |
|
acetate--CoA ligase; Provisional
Pssm-ID: 215553 [Multi-domain] Cd Length: 728 Bit Score: 195.30 E-value: 2.30e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 26 YQHSIENPDEFWA---EQAKIVDWIKPfTQVKNTSfDKDNFKIEWFADGELNVSANCLDRHLKEHPHKPAIIW--EGDHP 100
Cdd:PLN03052 126 QRFSVENPEVYWSivlDELSLVFSVPP-RCILDTS-DESNPGGQWLPGAVLNVAECCLTPKPSKTDDSIAIIWrdEGSDD 203
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 101 SRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKL 180
Cdd:PLN03052 204 LPVNRMTLSELRSQVSRVANALDALGFEKGDAIAIDMPMNVHAVIIYLAIILAGCVVVSIADSFAPSEIATRLKISKAKA 283
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 181 VITADSSLRAGKLLPLKENV-----DLALALPGTECveNVIVVYRNAnpiemkpgrDLWYHLIIMEVDANCPPEPMKA-- 253
Cdd:PLN03052 284 IFTQDVIVRGGKSIPLYSRVveakaPKAIVLPADGK--SVRVKLREG---------DMSWDDFLARANGLRRPDEYKAve 352
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 254 ---EDPLFILYTSGSTGKPKGVLHTTGGYLVYVASTFKEVfDLKQDDVY-WCTaDVGWITGHsYLIYGPLANGTTTLMFE 329
Cdd:PLN03052 353 qpvEAFTNILFSSGTTGEPKAIPWTQLTPLRAAADAWAHL-DIRKGDIVcWPT-NLGWMMGP-WLVYASLLNGATLALYN 429
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 330 GVPQYPTWARLghvVDKHKVSILYTAPTAIRAMMREG--DSYvresNRSSLRLLGSVGEPINPEAWNW-----YYNvvge 402
Cdd:PLN03052 430 GSPLGRGFAKF---VQDAKVTMLGTVPSIVKTWKNTNcmAGL----DWSSIRCFGSTGEASSVDDYLWlmsraGYK---- 498
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 403 grcPIVDTWWQTETGGILIAplpgATALKP---GSATRPLFGIQPAIVDGEGNEL--EGAAEGNLVIKDSWPGQMRTIWg 477
Cdd:PLN03052 499 ---PIIEYCGGTELGGGFVT----GSLLQPqafAAFSTPAMGCKLFILDDSGNPYpdDAPCTGELALFPLMFGASSTLL- 570
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 478 DPDRFiEAYFS---TFKNTYFT--GDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESAL-VSHEAVAEAAVVGMPHd 551
Cdd:PLN03052 571 NADHY-KVYFKgmpVFNGKILRrhGDIFERTSGGYYRAHGRADDTMNLGGIKVSSVEIERVCnAADESVLETAAIGVPP- 648
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446925704 552 iKGQG---ICTFVTLQA---GVPESEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKIAANEL 621
Cdd:PLN03052 649 -PGGGpeqLVIAAVLKDppgSNPDLNELKKIFNSAIQKKLNPLFKVSAVVIVPSFPRTASNKVMRRVLRQQLAQEL 723
|
|
| PRK08314 |
PRK08314 |
long-chain-fatty-acid--CoA ligase; Validated |
73-613 |
4.22e-53 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236235 [Multi-domain] Cd Length: 546 Bit Score: 190.94 E-value: 4.22e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 73 LNVSANcldrhlkEHPHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLK-KYGIGKGDRVVLYMPMVTEAAIAMLACA 151
Cdd:PRK08314 16 LEVSAR-------RYPDKTAIVFYGRA------ISYRELLEEAERLAGYLQqECGVRKGDRVLLYMQNSPQFVIAYYAIL 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 152 RIGAVHCVVFGGFSPDSLASRIEDSQAKLVITAdSSLrAGKLLPLKenvdlalalpGTECVENVIVV-YRNANP------ 224
Cdd:PRK08314 83 RANAVVVPVNPMNREEELAHYVTDSGARVAIVG-SEL-APKVAPAV----------GNLRLRHVIVAqYSDYLPaepeia 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 225 --------IEMKPGRDLWYHLIIMEVDANCPPEPMKA--EDPLFILYTSGSTGKPKGVLHTTGGYLVYVASTfKEVFDLK 294
Cdd:PRK08314 151 vpawlraePPLQALAPGGVVAWKEALAAGLAPPPHTAgpDDLAVLPYTSGTTGVPKGCMHTHRTVMANAVGS-VLWSNST 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 295 QDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFegvpqyPTWAR--LGHVVDKHKVsilyTAPTAIRAMMRE--GDSYV 370
Cdd:PRK08314 230 PESVVLAVLPLFHVTGMVHSMNAPIYAGATVVLM------PRWDReaAARLIERYRV----THWTNIPTMVVDflASPGL 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 371 RESNRSSLRLLGSVGEPInPEAwnwyynvVGE---GRC--PIVDTWWQTETggilIAPlpgaTALKPGSATR------PL 439
Cdd:PRK08314 300 AERDLSSLRYIGGGGAAM-PEA-------VAErlkELTglDYVEGYGLTET----MAQ----THSNPPDRPKlqclgiPT 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 440 FGIQPAIVDGE-GNELEGAAEGNLVIkdSWPGQMRTIWGDPDRFIEAYFSTFKNTYF-TGDGARRDEDGYYWITGRVDDV 517
Cdd:PRK08314 364 FGVDARVIDPEtLEELPPGEVGEIVV--HGPQVFKGYWNRPEATAEAFIEIDGKRFFrTGDLGRMDEEGYFFITDRLKRM 441
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 518 LNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPE--SEElrkELISWVRKVLGPVASPDALH 595
Cdd:PRK08314 442 INASGFKVWPAEVENLLYKHPAIQEACVIATPDPRRGETVKAVVVLRPEARGktTEE---EIIAWAREHMAAYKYPRIVE 518
|
570
....*....|....*...
gi 446925704 596 WAPALPKTRSGKIMRRIL 613
Cdd:PRK08314 519 FVDSLPKSGSGKILWRQL 536
|
|
| Firefly_Luc_like |
cd05911 |
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ... |
104-609 |
5.43e-53 |
|
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341237 [Multi-domain] Cd Length: 486 Bit Score: 189.35 E-value: 5.43e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 104 KIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVIT 183
Cdd:cd05911 9 KELTYAQLRTLSRRLAAGLRKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKPKVIFT 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 184 ADSSLragkllplkENVDLALALPGTecVENVIVVyrNANPIEMKPGRDLWYHLIIMEVDANCPPEPMKAEDPLFILYTS 263
Cdd:cd05911 89 DPDGL---------EKVKEAAKELGP--KDKIIVL--DDKPDGVLSIEDLLSPTLGEEDEDLPPPLKDGKDDTAAILYSS 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 264 GSTGKPKGVLHTTGGYLVYVASTFKEVFDLKQ-DDVYWCTADVGWITGHSYLIYGPLaNGTTTLMFEGvPQYPTWARLgh 342
Cdd:cd05911 156 GTTGLPKGVCLSHRNLIANLSQVQTFLYGNDGsNDVILGFLPLYHIYGLFTTLASLL-NGATVIIMPK-FDSELFLDL-- 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 343 vVDKHKVSILYTAPtAIRAMMREGDSYVREsNRSSLRLLGSVGEPINPEAwnwyYNVVGE--GRCPIVDTWWQTETGGIL 420
Cdd:cd05911 232 -IEKYKITFLYLVP-PIAAALAKSPLLDKY-DLSSLRVILSGGAPLSKEL----QELLAKrfPNATIKQGYGMTETGGIL 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 421 IAPLPGAtaLKPGSATRPLFGIQPAIVDGEGNELEGAAE-GNLVIKDswPGQMRTIWGDPdrfiEAYFSTFKNTYF--TG 497
Cdd:cd05911 305 TVNPDGD--DKPGSVGRLLPNVEAKIVDDDGKDSLGPNEpGEICVRG--PQVMKGYYNNP----EATKETFDEDGWlhTG 376
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 498 DGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGvPESEElrKEL 577
Cdd:cd05911 377 DIGYFDEDGYLYIVDRKKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGIPDEVSGELPRAYVVRKPG-EKLTE--KEV 453
|
490 500 510
....*....|....*....|....*....|....*.
gi 446925704 578 ISWVRKvlgPVASPDALH----WAPALPKTRSGKIM 609
Cdd:cd05911 454 KDYVAK---KVASYKQLRggvvFVDEIPKSASGKIL 486
|
|
| AA-adenyl-dom |
TIGR01733 |
amino acid adenylation domain; This model represents a domain responsible for the specific ... |
107-545 |
6.55e-53 |
|
amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.
Pssm-ID: 273779 [Multi-domain] Cd Length: 409 Bit Score: 187.09 E-value: 6.55e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 107 SYKELHDEVCRFANVLKKY-GIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITaD 185
Cdd:TIGR01733 1 TYRELDERANRLARHLRAAgGVGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLLLT-D 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 186 SSLRAgkllplkenvDLALALPGTECVENVIVVYRNANPIEMKPgrdlwyhliimevdancpPEPMKAEDPLFILYTSGS 265
Cdd:TIGR01733 80 SALAS----------RLAGLVLPVILLDPLELAALDDAPAPPPP------------------DAPSGPDDLAYVIYTSGS 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 266 TGKPKGVLHTTGGyLVYVASTFKEVFDLKQDDVYWCTADVGWitGHSYL-IYGPLANGTTTLMFEGVPQYPTWARLGHVV 344
Cdd:TIGR01733 132 TGRPKGVVVTHRS-LVNLLAWLARRYGLDPDDRVLQFASLSF--DASVEeIFGALLAGATLVVPPEDEERDDAALLAALI 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 345 DKHKVSILYTAPTAIRAMMREGdsyvrESNRSSLRLLGSVGEPINPEAWNWYYNVVGEGRcpIVDTWWQTE-TGGILIAP 423
Cdd:TIGR01733 209 AEHPVTVLNLTPSLLALLAAAL-----PPALASLRLVILGGEALTPALVDRWRARGPGAR--LINLYGPTEtTVWSTATL 281
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 424 LPGATALKPGSAT--RPLFGIQPAIVDGEGNELEGAAEGNLVIkdSWPGQMRTIWGDPD----RFIEAYFSTFKNT--YF 495
Cdd:TIGR01733 282 VDPDDAPRESPVPigRPLANTRLYVLDDDLRPVPVGVVGELYI--GGPGVARGYLNRPEltaeRFVPDPFAGGDGArlYR 359
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|
gi 446925704 496 TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAV 545
Cdd:TIGR01733 360 TGDLVRYLPDGNLEFLGRIDDQVKIRGYRIELGEIEAALLRHPGVREAVV 409
|
|
| CHC_CoA_lg |
cd05903 |
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ... |
106-615 |
8.74e-50 |
|
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.
Pssm-ID: 341229 [Multi-domain] Cd Length: 437 Bit Score: 179.50 E-value: 8.74e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 106 VSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITAD 185
Cdd:cd05903 2 LTYSELDTRADRLAAGLAALGVGPGDVVAFQLPNWWEFAVLYLACLRIGAVTNPILPFFREHELAFILRRAKAKVFVVPE 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 186 SslragkllplkenvdlalalpgtecvenvivvYRNANPIEMkPGrdlwyhliimevdancppepmkaeDPLFILYTSGS 265
Cdd:cd05903 82 R--------------------------------FRQFDPAAM-PD------------------------AVALLLFTSGT 104
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 266 TGKPKGVLHTTGGyLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFEgvpqypTW--ARLGHV 343
Cdd:cd05903 105 TGEPKGVMHSHNT-LSASIRQYAERLGLGPGDVFLVASPMAHQTGFVYGFTLPLLLGAPVVLQD------IWdpDKALAL 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 344 VDKHKVSILYTAPTAIRAMMREGDSYVRESnrSSLRLLGSVGEPINP----EAWNWYYNVVgegrcpiVDTWWQTETGGI 419
Cdd:cd05903 178 MREHGVTFMMGATPFLTDLLNAVEEAGEPL--SRLRTFVCGGATVPRslarRAAELLGAKV-------CSAYGSTECPGA 248
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 420 LIAPLPGATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDswPGQMRTIWGDPDRFIEAYFSTFkntYFTGDG 499
Cdd:cd05903 249 VTSITPAPEDRRLYTDGRPLPGVEIKVVDDTGATLAPGVEGELLSRG--PSVFLGYLDRPDLTADAAPEGW---FRTGDL 323
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 500 ARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAG-VPESEELRKELi 578
Cdd:cd05903 324 ARLDEDGYLRITGRSKDIIIRGGENIPVLEVEDLLLGHPGVIEAAVVALPDERLGERACAVVVTKSGaLLTFDELVAYL- 402
|
490 500 510
....*....|....*....|....*....|....*..
gi 446925704 579 swVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRK 615
Cdd:cd05903 403 --DRQGVAKQYWPERLVHVDDLPRTPSGKVQKFRLRE 437
|
|
| 4CL |
cd05904 |
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ... |
85-613 |
2.71e-46 |
|
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.
Pssm-ID: 341230 [Multi-domain] Cd Length: 505 Bit Score: 171.26 E-value: 2.71e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 85 KEHPHKPAIIwegdHPSRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGF 164
Cdd:cd05904 16 SAHPSRPALI----DAATGRALTYAELERRVRRLAAGLAKRGGRKGDVVLLLSPNSIEFPVAFLAVLSLGAVVTTANPLS 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 165 SPDSLASRIEDSQAKLVITadSSLRAGKLLplkenvdlALALPgtecvenVIVVYRNANPiemkpgRDLWYHLIIMEVDA 244
Cdd:cd05904 92 TPAEIAKQVKDSGAKLAFT--TAELAEKLA--------SLALP-------VVLLDSAEFD------SLSFSDLLFEADEA 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 245 NCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVAST-FKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGT 323
Cdd:cd05904 149 EPPVVVIKQDDVAALLYSSGTTGRSKGVMLTHRNLIAMVAQFvAGEGSNSDSEDVFLCVLPMFHIYGLSSFALGLLRLGA 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 324 TTLMfegVPQYPTWARLGhVVDKHKVSILYTAPTAIRAMMR--EGDSYvresNRSSLRLLGSVGEPINPEawnwyynvVG 401
Cdd:cd05904 229 TVVV---MPRFDLEELLA-AIERYKVTHLPVVPPIVLALVKspIVDKY----DLSSLRQIMSGAAPLGKE--------LI 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 402 EG------RCPIVDTWWQTETGGILIA-PLPGATALKPGSATRPLFGIQPAIVDGEGNELEGAAE-GNLVIKDswPGQMR 473
Cdd:cd05904 293 EAfrakfpNVDLGQGYGMTESTGVVAMcFAPEKDRAKYGSVGRLVPNVEAKIVDPETGESLPPNQtGELWIRG--PSIMK 370
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 474 TIWGDPdrfiEAYFSTFKNTYF--TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHD 551
Cdd:cd05904 371 GYLNNP----EATAATIDKEGWlhTGDLCYIDEDGYLFIVDRLKELIKYKGFQVAPAELEALLLSHPEILDAAVIPYPDE 446
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 446925704 552 IKGQGICTFVTLQagvPESEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRIL 613
Cdd:cd05904 447 EAGEVPMAFVVRK---PGSSLTEDEIMDFVAKQVAPYKKVRKVAFVDAIPKSPSGKILRKEL 505
|
|
| A_NRPS_Srf_like |
cd12117 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ... |
81-613 |
3.88e-46 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.
Pssm-ID: 341282 [Multi-domain] Cd Length: 483 Bit Score: 170.46 E-value: 3.88e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 81 DRHLKEHPHKPAIIWEGdhpsrhKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVV 160
Cdd:cd12117 4 EEQAARTPDAVAVVYGD------RSLTYAELNERANRLARRLRAAGVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPL 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 161 FGGFSPDSLASRIEDSQAKLVITADSslragkllplkenvdLALALPGTECVenvivvyrnanpiemkpgrdLWYHLIIM 240
Cdd:cd12117 78 DPELPAERLAFMLADAGAKVLLTDRS---------------LAGRAGGLEVA--------------------VVIDEALD 122
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 241 EVDANCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGylvyVASTFKEV--FDLKQDDVYWCTADVGWiTGHSYLIYGP 318
Cdd:cd12117 123 AGPAGNPAVPVSPDDLAYVMYTSGSTGRPKGVAVTHRG----VVRLVKNTnyVTLGPDDRVLQTSPLAF-DASTFEIWGA 197
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 319 LANGTT-TLMFEGVPQYPTwaRLGHVVDKHKVSILYTAPTAIRAMMREgdsyvRESNRSSLRLLGSVGEPINPEAWNWYY 397
Cdd:cd12117 198 LLNGARlVLAPKGTLLDPD--ALGALIAEEGVTVLWLTAALFNQLADE-----DPECFAGLRELLTGGEVVSPPHVRRVL 270
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 398 NVVGEGRcpIVDTWWQTETGGI----LIAPL-PGATALKPGsatRPLFGIQPAIVDGEGNELEGAAEGNLVIkdSWPGQM 472
Cdd:cd12117 271 AACPGLR--LVNGYGPTENTTFttshVVTELdEVAGSIPIG---RPIANTRVYVLDEDGRPVPPGVPGELYV--GGDGLA 343
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 473 RTIWGDPD----RFIEAYFSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGM 548
Cdd:cd12117 344 LGYLNRPAltaeRFVADPFGPGERLYRTGDLARWLPDGRLEFLGRIDDQVKIRGFRIELGEIEAALRAHPGVREAVVVVR 423
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 446925704 549 PHDIKGQGICTFVTLQAGVPeSEELRKelisWVRKVLGPVASPDALHWAPALPKTRSGKIMRRIL 613
Cdd:cd12117 424 EDAGGDKRLVAYVVAEGALD-AAELRA----FLRERLPAYMVPAAFVVLDELPLTANGKVDRRAL 483
|
|
| ligase_PEP_1 |
TIGR03098 |
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an ... |
88-615 |
3.64e-45 |
|
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an AMP-binding domain (pfam00501) associated with acyl CoA-ligases. These proteins are generally found in genomes containing the exosortase/PEP-CTERM protein expoert system, specifically the type 1 variant of this system described by the Genome Property GenProp0652. When found in this context they are invariably present next to a decarboxylase enzyme. A number of sequences from Burkholderia species also hit this model, but the genomic context is obviously different. The hypothesis of a constant substrate for this family is only strong where the exosortase context is present.
Pssm-ID: 211788 [Multi-domain] Cd Length: 517 Bit Score: 168.42 E-value: 3.64e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 88 PHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPD 167
Cdd:TIGR03098 14 PDATALVHHDRT------LTYAALSERVLALASGLRGLGLARGERVAIYLDKRLETVTAMFGAALAGGVFVPINPLLKAE 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 168 SLASRIEDSQAKLVITADSSLRAgkllplkenvdLALALPGTECVENVIVVYRNANPIEMKPGRDL--WYHLIIMEVDAn 245
Cdd:TIGR03098 88 QVAHILADCNVRLLVTSSERLDL-----------LHPALPGCHDLRTLIIVGDPAHASEGHPGEEPasWPKLLALGDAD- 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 246 cPPEPMKAEDPLFILYTSGSTGKPKGVL--HTTggyLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGT 323
Cdd:TIGR03098 156 -PPHPVIDSDMAAILYTSGSTGRPKGVVlsHRN---LVAGAQSVATYLENRPDDRLLAVLPLSFDYGFNQLTTAFYVGAT 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 324 TTLMfegvpQYPTWARLGHVVDKHKVSILYTAPtAIRAMMREGDsyVRESNRSSLRLLGSVGEPINPEAWNWYYNVVGEG 403
Cdd:TIGR03098 232 VVLH-----DYLLPRDVLKALEKHGITGLAAVP-PLWAQLAQLD--WPESAAPSLRYLTNSGGAMPRATLSRLRSFLPNA 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 404 RcpIVDTWWQTETGGILIAPlPGATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDSWPGQmrTIWGDPDRFI 483
Cdd:TIGR03098 304 R--LFLMYGLTEAFRSTYLP-PEEVDRRPDSIGKAIPNAEVLVLREDGSECAPGEEGELVHRGALVAM--GYWNDPEKTA 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 484 EAY---------FSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKG 554
Cdd:TIGR03098 379 ERFrplppfpgeLHLPELAVWSGDTVRRDEEGFLYFVGRRDEMIKTSGYRVSPTEVEEVAYATGLVAEAVAFGVPDPTLG 458
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 446925704 555 QGICTFVTLQAGVPESeelRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRK 615
Cdd:TIGR03098 459 QAIVLVVTPPGGEELD---RAALLAECRARLPNYMVPALIHVRQALPRNANGKIDRKALAK 516
|
|
| EntE |
COG1021 |
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ... |
80-618 |
5.15e-45 |
|
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440644 [Multi-domain] Cd Length: 533 Bit Score: 168.40 E-value: 5.15e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIwEGDHpsRHkivSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVhcV 159
Cdd:COG1021 31 LRRRAERHPDRIAVV-DGER--RL---SYAELDRRADRLAAGLLALGLRPGDRVVVQLPNVAEFVIVFFALFRAGAI--P 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFggfspdSLAS-R-------IEDSQAKLVITADsslRAGKLlplkENVDLALAL-PGTECVENVIVVYRNANPIEmkpg 230
Cdd:COG1021 103 VF------ALPAhRraeishfAEQSEAVAYIIPD---RHRGF----DYRALARELqAEVPSLRHVLVVGDAGEFTS---- 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 231 rdlWYHLIIMEVDANcPPEPmKAEDPLFILYTSGSTGKPKGVLHTTGGYLvYVASTFKEVFDLKQDDVYWCTADVGwitg 310
Cdd:COG1021 166 ---LDALLAAPADLS-EPRP-DPDDVAFFQLSGGTTGLPKLIPRTHDDYL-YSVRASAEICGLDADTVYLAALPAA---- 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 311 HSYL-----IYGPLANGTTTLMfEGVPqYPTWA-RLghvVDKHKVSILYTAPTAIRAMMREGDSyvRESNRSSLRLLGSV 384
Cdd:COG1021 236 HNFPlsspgVLGVLYAGGTVVL-APDP-SPDTAfPL---IERERVTVTALVPPLALLWLDAAER--SRYDLSSLRVLQVG 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 385 GEPINPEAWN--------WYYNVVG--EGRC----PIVDTWWQTETGGiliaplpgatalkpgsatRPlfgIQPA----I 446
Cdd:COG1021 309 GAKLSPELARrvrpalgcTLQQVFGmaEGLVnytrLDDPEEVILTTQG------------------RP---ISPDdevrI 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 447 VDGEGNELEGAAEGNLVIKDswPGqmrTIWG------------DPDRFieayfstfkntYFTGDGARRDEDGYYWITGRV 514
Cdd:COG1021 368 VDEDGNPVPPGEVGELLTRG--PY---TIRGyyrapehnarafTPDGF-----------YRTGDLVRRTPDGYLVVEGRA 431
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 515 DDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVtlqagVPESEELR-KELISWVRKvLGpVAS--- 590
Cdd:COG1021 432 KDQINRGGEKIAAEEVENLLLAHPAVHDAAVVAMPDEYLGERSCAFV-----VPRGEPLTlAELRRFLRE-RG-LAAfkl 504
|
570 580
....*....|....*....|....*...
gi 446925704 591 PDALHWAPALPKTRSGKIMRRILRKIAA 618
Cdd:COG1021 505 PDRLEFVDALPLTAVGKIDKKALRAALA 532
|
|
| PRK07529 |
PRK07529 |
AMP-binding domain protein; Validated |
80-618 |
5.17e-45 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236043 [Multi-domain] Cd Length: 632 Bit Score: 170.14 E-value: 5.17e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIW--EGDHPSRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVh 157
Cdd:PRK07529 31 LSRAAARHPDAPALSFllDADPLDRPETWTYAELLADVTRTANLLHSLGVGPGDVVAFLLPNLPETHFALWGGEAAGIA- 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 158 CVVFGGFSPDSLASRIEDSQAKLVITADSSLRA----------GKLLPLKE--NVDLALALPGTECVENVIVVYRNANPI 225
Cdd:PRK07529 110 NPINPLLEPEQIAELLRAAGAKVLVTLGPFPGTdiwqkvaevlAALPELRTvvEVDLARYLPGPKRLAVPLIRRKAHARI 189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 226 -----EMKPGRDlwyhliimevDANCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGyLVYVASTFKEVFDLKQDDVYW 300
Cdd:PRK07529 190 ldfdaELARQPG----------DRLFSGRPIGPDDVAAYFHTGGTTGMPKLAQHTHGN-EVANAWLGALLLGLGPGDTVF 258
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 301 C------------TADVGWITGHSYLIYGPLANGTTTLMfegvpqyptwARLGHVVDKHKVSILYTAPTAIRAMMregDS 368
Cdd:PRK07529 259 CglplfhvnallvTGLAPLARGAHVVLATPQGYRGPGVI----------ANFWKIVERYRINFLSGVPTVYAALL---QV 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 369 YVRESNRSSLRLLGSVGEPINPEAWNWYYNVVGegrCPIVDTWWQTE-TGGILIAPLPGAtaLKPGSATRPLFGIQPAIV 447
Cdd:PRK07529 326 PVDGHDISSLRYALCGAAPLPVEVFRRFEAATG---VRIVEGYGLTEaTCVSSVNPPDGE--RRIGSVGLRLPYQRVRVV 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 448 --DGEGNELEGAAE---GNLVIkdSWPG---------QMRTIWGDPDRFIeayfstfkntyfTGDGARRDEDGYYWITGR 513
Cdd:PRK07529 401 ilDDAGRYLRDCAVdevGVLCI--AGPNvfsgyleaaHNKGLWLEDGWLN------------TGDLGRIDADGYFWLTGR 466
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 514 VDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEElrkELISWVRK-VLGPVASPD 592
Cdd:PRK07529 467 AKDLIIRGGHNIDPAAIEEALLRHPAVALAAAVGRPDAHAGELPVAYVQLKPGASATEA---ELLAFARDhIAERAAVPK 543
|
570 580
....*....|....*....|....*.
gi 446925704 593 ALHWAPALPKTRSGKIMRRILRKIAA 618
Cdd:PRK07529 544 HVRILDALPKTAVGKIFKPALRRDAI 569
|
|
| A_NRPS_PvdJ-like |
cd17649 |
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ... |
88-614 |
6.83e-45 |
|
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341304 [Multi-domain] Cd Length: 450 Bit Score: 166.00 E-value: 6.83e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 88 PHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPD 167
Cdd:cd17649 1 PDAVALVFGDQS------LSYAELDARANRLAHRLRALGVGPEVRVGIALERSLEMVVALLAILKAGGAYVPLDPEYPAE 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 168 SLASRIEDSQAKLVITADsslragkllplkenvdlalalpgtecvenvivvyrnanpiemkpGRDLWYhliimevdancp 247
Cdd:cd17649 75 RLRYMLEDSGAGLLLTHH--------------------------------------------PRQLAY------------ 98
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 248 pepmkaedplfILYTSGSTGKPKGVLhTTGGYLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLiYGPLANGTTTLM 327
Cdd:cd17649 99 -----------VIYTSGSTGTPKGVA-VSHGPLAAHCQATAERYGLTPGDRELQFASFNFDGAHEQL-LPPLICGACVVL 165
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 328 fEGVPQYPTWARLGHVVDKHKVSILYTAPTAIRAMMREGDSyVRESNRSSLRLLGSVGEPINPE-AWNWyynvvGEGRCP 406
Cdd:cd17649 166 -RPDELWASADELAEMVRELGVTVLDLPPAYLQQLAEEADR-TGDGRPPSLRLYIFGGEALSPElLRRW-----LKAPVR 238
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 407 IVDTWWQTETggiLIAPL-----PGATALKPGSAT-RPLFGIQPAIVDGEGNELEGAAEGNLVIkdSWPGQMRTIWGDP- 479
Cdd:cd17649 239 LFNAYGPTEA---TVTPLvwkceAGAARAGASMPIgRPLGGRSAYILDADLNPVPVGVTGELYI--GGEGLARGYLGRPe 313
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 480 ---DRFIE-AYFSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQ 555
Cdd:cd17649 314 ltaERFVPdPFGAPGSRLYRTGDLARWRDDGVIEYLGRVDHQVKIRGFRIELGEIEAALLEHPGVREAAVVALDGAGGKQ 393
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 446925704 556 gICTFVTLQAGVPEsEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILR 614
Cdd:cd17649 394 -LVAYVVLRAAAAQ-PELRAQLRTALRASLPDYMVPAHLVFLARLPLTPNGKLDRKALP 450
|
|
| ttLC_FACS_AlkK_like |
cd12119 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
80-615 |
1.43e-44 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.
Pssm-ID: 341284 [Multi-domain] Cd Length: 518 Bit Score: 166.65 E-value: 1.43e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPaiIWEGDHPSRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCV 159
Cdd:cd12119 2 LEHAARLHGDRE--IVSRTHEGEVHRYTYAEVAERARRLANALRRLGVKPGDRVATLAWNTHRHLELYYAVPGMGAVLHT 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFGGFSPDSLASRIEDSQAKLVITADSslragkLLPLKENVdlalaLPGTECVENVIVVYRNANPIEMKPGRDLWYHLII 239
Cdd:cd12119 80 INPRLFPEQIAYIINHAEDRVVFVDRD------FLPLLEAI-----APRLPTVEHVVVMTDDAAMPEPAGVGVLAYEELL 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 240 MEVDANCPPEPMKAEDPLFILYTSGSTGKPKGV--------LHTtggylvyVASTFKEVFDLKQDDVY-----------W 300
Cdd:cd12119 149 AAESPEYDWPDFDENTAAAICYTSGTTGNPKGVvyshrslvLHA-------MAALLTDGLGLSESDVVlpvvpmfhvnaW 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 301 CTADVGWITGHSYLIYGPlangtttlmfegvpqYPTWARLGHVVDKHKVSILYTAPTAIRAMMREGDSYVREsnRSSLRL 380
Cdd:cd12119 222 GLPYAAAMVGAKLVLPGP---------------YLDPASLAELIEREGVTFAAGVPTVWQGLLDHLEANGRD--LSSLRR 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 381 L---GSVGEPINPEAWNWYYnvvgegrCPIVDTWWQTETGGILIA--PLPGATALKPG-------SATRPLFGIQPAIVD 448
Cdd:cd12119 285 VvigGSAVPRSLIEAFEERG-------VRVIHAWGMTETSPLGTVarPPSEHSNLSEDeqlalraKQGRPVPGVELRIVD 357
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 449 GEGNELE--GAAEGNLVIKDSW--PGQMRtiwgdPDRFIEAYFStfkNTYF-TGDGARRDEDGYYWITGRVDDVLNVSGH 523
Cdd:cd12119 358 DDGRELPwdGKAVGELQVRGPWvtKSYYK-----NDEESEALTE---DGWLrTGDVATIDEDGYLTITDRSKDVIKSGGE 429
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 524 RLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQagvPESEELRKELISWVRKVLGPVASPDALHWAPALPKT 603
Cdd:cd12119 430 WISSVELENAIMAHPAVAEAAVIGVPHPKWGERPLAVVVLK---EGATVTAEELLEFLADKVAKWWLPDDVVFVDEIPKT 506
|
570
....*....|..
gi 446925704 604 RSGKIMRRILRK 615
Cdd:cd12119 507 STGKIDKKALRE 518
|
|
| A_NRPS_VisG_like |
cd17651 |
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ... |
82-614 |
2.97e-44 |
|
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341306 [Multi-domain] Cd Length: 491 Bit Score: 165.21 E-value: 2.97e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 82 RHLKEHPHKPAIIWEGDhpsrhkIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVF 161
Cdd:cd17651 3 RQAARTPDAPALVAEGR------RLTYAELDRRANRLAHRLRARGVGPGDLVALCARRSAELVVALLAILKAGAAYVPLD 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 162 GGFSPDSLASRIEDSQAKLVITADsslRAGKLLPLKENVDLALALPGTecvenvivvyrnANPIEMKPGRDLwyhliime 241
Cdd:cd17651 77 PAYPAERLAFMLADAGPVLVLTHP---ALAGELAVELVAVTLLDQPGA------------AAGADAEPDPAL-------- 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 242 vdancppepmKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVASTfKEVFDLKQDDVYWCTADVG--------WitghSY 313
Cdd:cd17651 134 ----------DADDLAYVIYTSGSTGRPKGVVMPHRSLANLVAWQ-ARASSLGPGARTLQFAGLGfdvsvqeiF----ST 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 314 LIYGplanGTTTLMFEGVPQYPtwARLGHVVDKHKVSILYTAPTAIRAMMREGDSYVRESnrSSLRLLGSVGEPINPEAW 393
Cdd:cd17651 199 LCAG----ATLVLPPEEVRTDP--PALAAWLDEQRISRVFLPTVALRALAEHGRPLGVRL--AALRYLLTGGEQLVLTED 270
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 394 NWYYNVvGEGRCPIVDTWWQTETGGILIAPLPGATALKPGSAT--RPLFGIQPAIVDGEGNELEGAAEGNLVIkdSWPGQ 471
Cdd:cd17651 271 LREFCA-GLPGLRLHNHYGPTETHVVTALSLPGDPAAWPAPPPigRPIDNTRVYVLDAALRPVPPGVPGELYI--GGAGL 347
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 472 MRTIWGDPD----RFIEAYFSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVG 547
Cdd:cd17651 348 ARGYLNRPEltaeRFVPDPFVPGARMYRTGDLARWLPDGELEFLGRADDQVKIRGFRIELGEIEAALARHPGVREAVVLA 427
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446925704 548 MPHDIKGQGICTFVTLQAGVPES-EELRKELiswvRKVLGPVASPDALHWAPALPKTRSGKIMRRILR 614
Cdd:cd17651 428 REDRPGEKRLVAYVVGDPEAPVDaAELRAAL----ATHLPEYMVPSAFVLLDALPLTPNGKLDRRALP 491
|
|
| PRK06188 |
PRK06188 |
acyl-CoA synthetase; Validated |
71-614 |
1.08e-43 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235731 [Multi-domain] Cd Length: 524 Bit Score: 164.39 E-value: 1.08e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 71 GELNVSAncldrhLKEHPHKPAIIWEGDhpsrhkIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAM--- 147
Cdd:PRK06188 15 GHLLVSA------LKRYPDRPALVLGDT------RLTYGQLADRISRYIQAFEALGLGTGDAVALLSLNRPEVLMAIgaa 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 148 -LACARIGAVHCVVfggfSPDSLASRIEDSQAKLVITADSSLragkllplkenVDLALAL----PGTECVENVivvyrna 222
Cdd:PRK06188 83 qLAGLRRTALHPLG----SLDDHAYVLEDAGISTLIVDPAPF-----------VERALALlarvPSLKHVLTL------- 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 223 NPIEMkpGRDLWYHLIIME----VDANCPPepmkaeDPLFILYTSGSTGKPKGVLHTTGGYLVYVAstfkevfdlkqddv 298
Cdd:PRK06188 141 GPVPD--GVDLLAAAAKFGpaplVAAALPP------DIAGLAYTGGTTGKPKGVMGTHRSIATMAQ-------------- 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 299 yWCTADVGWITGHSYLIYGPLANGTttlmfeGVPQYPTWARLGHV--------------VDKHKVSILYTAPTAIRAMMR 364
Cdd:PRK06188 199 -IQLAEWEWPADPRFLMCTPLSHAG------GAFFLPTLLRGGTVivlakfdpaevlraIEEQRITATFLVPTMIYALLD 271
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 365 EGDSyvRESNRSSLRLLGSVGEPINP----EAwnwyYNVVGegrcPI-VDTWWQTEtggiliAPLPgATALKP------- 432
Cdd:PRK06188 272 HPDL--RTRDLSSLETVYYGASPMSPvrlaEA----IERFG----PIfAQYYGQTE------APMV-ITYLRKrdhdpdd 334
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 433 ----GSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDswPGQMRTIWGDPDRFIEayfsTFKNTYF-TGDGARRDEDGY 507
Cdd:PRK06188 335 pkrlTSCGRPTPGLRVALLDEDGREVAQGEVGEICVRG--PLVMDGYWNRPEETAE----AFRDGWLhTGDVAREDEDGF 408
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 508 YWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEElrkELISWVRKVLGP 587
Cdd:PRK06188 409 YYIVDRKKDMIVTGGFNVFPREVEDVLAEHPAVAQVAVIGVPDEKWGEAVTAVVVLRPGAAVDAA---ELQAHVKERKGS 485
|
570 580
....*....|....*....|....*..
gi 446925704 588 VASPDALHWAPALPKTRSGKIMRRILR 614
Cdd:PRK06188 486 VHAPKQVDFVDSLPLTALGKPDKKALR 512
|
|
| MCS |
cd05941 |
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ... |
89-615 |
1.44e-43 |
|
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.
Pssm-ID: 341264 [Multi-domain] Cd Length: 442 Bit Score: 162.07 E-value: 1.44e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 89 HKPAIIWEGdhpsrhKIVSYKELHDEVCRFANVL-KKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPD 167
Cdd:cd05941 1 DRIAIVDDG------DSITYADLVARAARLANRLlALGKDLRGDRVAFLAPPSAEYVVAQLAIWRAGGVAVPLNPSYPLA 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 168 SLASRIEDSQAKLVItadsslragkllplkenvDLALalpgtecvenvivvyrnanpiemkpgrdlwyhliimevdancp 247
Cdd:cd05941 75 ELEYVITDSEPSLVL------------------DPAL------------------------------------------- 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 248 pepmkaedplfILYTSGSTGKPKGVLHTTGGylvyVASTFKEVFDLKQddvyWCTADV-----------GWITGhsylIY 316
Cdd:cd05941 94 -----------ILYTSGTTGRPKGVVLTHAN----LAANVRALVDAWR----WTEDDVllhvlplhhvhGLVNA----LL 150
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 317 GPLANGTTTLMfegVPQYPTWARLGHVVDKHkVSILYTAPT-------AIRAMMREGDSYVRESNRSsLRLLGSVGEPIN 389
Cdd:cd05941 151 CPLFAGASVEF---LPKFDPKEVAISRLMPS-ITVFMGVPTiytrllqYYEAHFTDPQFARAAAAER-LRLMVSGSAALP 225
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 390 PEAWNWYYNVVGEgrcPIVDTWWQTETGGILIAPLPGATalKPGSATRPLFGIQPAIVDGEGNE-LEGAAEGNLVIKDsw 468
Cdd:cd05941 226 VPTLEEWEAITGH---TLLERYGMTEIGMALSNPLDGER--RPGTVGMPLPGVQARIVDEETGEpLPRGEVGEIQVRG-- 298
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 469 PGQMRTIWGDPDRFIEAYfsTFKNTYFTGDGARRDEDGYYWITGRV-DDVLNVSGHRLGTAEIESALVSHEAVAEAAVVG 547
Cdd:cd05941 299 PSVFKEYWNKPEATKEEF--TDDGWFKTGDLGVVDEDGYYWILGRSsVDIIKSGGYKVSALEIERVLLAHPGVSECAVIG 376
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446925704 548 MPHDIKGQGICTFVTLQAGVPESEELrkELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRK 615
Cdd:cd05941 377 VPDPDWGERVVAVVVLRAGAAALSLE--ELKEWAKQRLAPYKRPRRLILVDELPRNAMGKVNKKELRK 442
|
|
| A_NRPS_AB3403-like |
cd17646 |
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ... |
80-613 |
1.90e-43 |
|
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341301 [Multi-domain] Cd Length: 488 Bit Score: 162.83 E-value: 1.90e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCV 159
Cdd:cd17646 4 VAEQAARTPDAPAVVDEGRT------LTYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLP 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFGGFSPDSLASRIEDSQAKLVIT-ADSSLRAGKLLPLKENVDLALALPgtecvenvivvyrnanpiemkPGRDlwyhli 238
Cdd:cd17646 78 LDPGYPADRLAYMLADAGPAVVLTtADLAARLPAGGDVALLGDEALAAP---------------------PATP------ 130
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 239 imevdancPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVAsTFKEVFDLKQDDVYWCTA----DV-GWitghsy 313
Cdd:cd17646 131 --------PLVPPRPDNLAYVIYTSGSTGRPKGVMVTHAGIVNRLL-WMQDEYPLGPGDRVLQKTplsfDVsVW------ 195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 314 LIYGPLANGTTTLMFE----GVPQYptwarLGHVVDKHKVSILYTAPTAIRAMMREGDSyvreSNRSSLRLLGSVGEPIN 389
Cdd:cd17646 196 ELFWPLVAGARLVVARpgghRDPAY-----LAALIREHGVTTCHFVPSMLRVFLAEPAA----GSCASLRRVFCSGEALP 266
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 390 PEAWNWYYNVVGegrCPIVDTWWQTETG-GILIAPLPGATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIkdSW 468
Cdd:cd17646 267 PELAARFLALPG---AELHNLYGPTEAAiDVTHWPVRGPAETPSVPIGRPVPNTRLYVLDDALRPVPVGVPGELYL--GG 341
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 469 PGQMRTIWGDP----DRFIEAYFSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAA 544
Cdd:cd17646 342 VQLARGYLGRPaltaERFVPDPFGPGSRMYRTGDLARWRPDGALEFLGRSDDQVKIRGFRVEPGEIEAALAAHPAVTHAV 421
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 446925704 545 VVGMPHDIKGQGICTFVTLQAGvpESEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRIL 613
Cdd:cd17646 422 VVARAAPAGAARLVGYVVPAAG--AAGPDTAALRAHLAERLPEYMVPAAFVVLDALPLTANGKLDRAAL 488
|
|
| PRK07786 |
PRK07786 |
long-chain-fatty-acid--CoA ligase; Validated |
77-615 |
5.18e-43 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 169098 [Multi-domain] Cd Length: 542 Bit Score: 162.64 E-value: 5.18e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 77 ANCLDRHLKEHPHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAV 156
Cdd:PRK07786 20 VNQLARHALMQPDAPALRFLGNT------TTWRELDDRVAALAGALSRRGVGFGDRVLILMLNRTEFVESVLAANMLGAI 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 157 HCVVFGGFSPDSLASRIEDSQAKLVITADSslragkLLPLKENVDLALALPGTecvenVIVVYRNANpiemkpGRDLWYH 236
Cdd:PRK07786 94 AVPVNFRLTPPEIAFLVSDCGAHVVVTEAA------LAPVATAVRDIVPLLST-----VVVAGGSSD------DSVLGYE 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 237 LIIMEVDANCPPEPMKAEDPLFILYTSGSTGKPKG-VL-HTTggyLVYVASTFKEVFDL-KQDDVYWCTADVGWITGHSY 313
Cdd:PRK07786 157 DLLAEAGPAHAPVDIPNDSPALIMYTSGTTGRPKGaVLtHAN---LTGQAMTCLRTNGAdINSDVGFVGVPLFHIAGIGS 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 314 LIYGPLANGTTTLmfegvpqYPTWA----RLGHVVDKHKVSILYTAPTAIRAMMREGDSYVREsnrSSLRLLGSVGEPIN 389
Cdd:PRK07786 234 MLPGLLLGAPTVI-------YPLGAfdpgQLLDVLEAEKVTGIFLVPAQWQAVCAEQQARPRD---LALRVLSWGAAPAS 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 390 PEAWNWYYNVVGEGRcpIVDTWWQTETGGILIApLPGATAL-KPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDsw 468
Cdd:PRK07786 304 DTLLRQMAATFPEAQ--ILAAFGQTEMSPVTCM-LLGEDAIrKLGSVGKVIPTVAARVVDENMNDVPVGEVGEIVYRA-- 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 469 PGQMRTIWGDPDRFIEAyfstFKNTYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVG 547
Cdd:PRK07786 379 PTLMSGYWNNPEATAEA----FAGGWFhSGDLVRQDEEGYVWVVDRKKDMIISGGENIYCAEVENVLASHPDIVEVAVIG 454
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 446925704 548 MPHDIKGQGICTFVTLQAGvpeSEELR-KELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRK 615
Cdd:PRK07786 455 RADEKWGEVPVAVAAVRND---DAALTlEDLAEFLTDRLARYKHPKALEIVDALPRNPAGKVLKTELRE 520
|
|
| EntF |
COG1020 |
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ... |
80-640 |
1.65e-42 |
|
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440643 [Multi-domain] Cd Length: 1329 Bit Score: 165.42 E-value: 1.65e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCV 159
Cdd:COG1020 482 FEAQAARTPDAVAVVFGDQS------LTYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAVLKAGAAYVP 555
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFGGFSPDSLASRIEDSQAKLVITaDSSLRAgkllplkenvdlalALPGTECveNVIVVyrnanpiemkpgrDlwyHLII 239
Cdd:COG1020 556 LDPAYPAERLAYMLEDAGARLVLT-QSALAA--------------RLPELGV--PVLAL-------------D---ALAL 602
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 240 MEVDANCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGyLVYVASTFKEVFDLKQDDVywctadVGWITGHS-----YL 314
Cdd:COG1020 603 AAEPATNPPVPVTPDDLAYVIYTSGSTGRPKGVMVEHRA-LVNLLAWMQRRYGLGPGDR------VLQFASLSfdasvWE 675
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 315 IYGPLANGTTTLMfegVPQYPTW--ARLGHVVDKHKVSILYTAPTAIRAMMREGdsyvrESNRSSLRLLGSVGEPINPEA 392
Cdd:COG1020 676 IFGALLSGATLVL---APPEARRdpAALAELLARHRVTVLNLTPSLLRALLDAA-----PEALPSLRLVLVGGEALPPEL 747
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 393 WNWYYNVVGEGR---------CPIVDTWWQTETGGILIAPLP-GatalkpgsatRPLFGIQPAIVDGEGN--------EL 454
Cdd:COG1020 748 VRRWRARLPGARlvnlygpteTTVDSTYYEVTPPDADGGSVPiG----------RPIANTRVYVLDAHLQpvpvgvpgEL 817
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 455 ----EGAAEGnlvikdswpgqmrtIWGDPD----RFIEAYFStFKNT--YFTGDGARRDEDG---YywiTGRVDDVLNVS 521
Cdd:COG1020 818 yiggAGLARG--------------YLNRPEltaeRFVADPFG-FPGArlYRTGDLARWLPDGnleF---LGRADDQVKIR 879
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 522 GHR--LGtaEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEELRKELiswVRKVLGPVASPDALHWAPA 599
Cdd:COG1020 880 GFRieLG--EIEAALLQHPGVREAVVVAREDAPGDKRLVAYVVPEAGAAAAAALLRLA---LALLLPPYMVPAAVVLLLP 954
|
570 580 590 600
....*....|....*....|....*....|....*....|.
gi 446925704 600 LPKTRSGKimrrILRKIAANELDSLGDTSTLAEPAVVDQLI 640
Cdd:COG1020 955 LPLTGNGK----LDRLALPAPAAAAAAAAAAPPAEEEEEEA 991
|
|
| PRK13295 |
PRK13295 |
cyclohexanecarboxylate-CoA ligase; Reviewed |
80-620 |
4.11e-42 |
|
cyclohexanecarboxylate-CoA ligase; Reviewed
Pssm-ID: 171961 [Multi-domain] Cd Length: 547 Bit Score: 160.22 E-value: 4.11e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAII---WEGDHPSRhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAV 156
Cdd:PRK13295 30 LDACVASCPDKTAVTavrLGTGAPRR---FTYRELAALVDRVAVGLARLGVGRGDVVSCQLPNWWEFTVLYLACSRIGAV 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 157 HCVVFGGFSPDSLASRIEDSQAKLVITA------DSSLRAGKLLPlkenvdlalALPGtecVENVIVV-YRNANPIE--- 226
Cdd:PRK13295 107 LNPLMPIFRERELSFMLKHAESKVLVVPktfrgfDHAAMARRLRP---------ELPA---LRHVVVVgGDGADSFEall 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 227 MKPGRDLWYHLIIMeVDANCPpepmKAEDPLFILYTSGSTGKPKGVLHTT----GGYLVYVastfkEVFDLKQDDVYWCT 302
Cdd:PRK13295 175 ITPAWEQEPDAPAI-LARLRP----GPDDVTQLIYTSGTTGEPKGVMHTAntlmANIVPYA-----ERLGLGADDVILMA 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 303 ADVGWITGHSYLIYGPLANGTTTLMFEgvpqypTW--ARLGHVVDKHKVSILYTAPTAIRAMMRegdsYVRESNR--SSL 378
Cdd:PRK13295 245 SPMAHQTGFMYGLMMPVMLGATAVLQD------IWdpARAAELIRTEGVTFTMASTPFLTDLTR----AVKESGRpvSSL 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 379 RLLGSVGEPInPEAwnwyynVVGEGR----CPIVDTWWQTETGGILIAPLPGATALKPGSATRPLFGIQPAIVDGEGNEL 454
Cdd:PRK13295 315 RTFLCAGAPI-PGA------LVERARaalgAKIVSAWGMTENGAVTLTKLDDPDERASTTDGCPLPGVEVRVVDADGAPL 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 455 EGAAEGNLVIK--DSWPGQM-RTIWGDPDrfIEAYFStfkntyfTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIE 531
Cdd:PRK13295 388 PAGQIGRLQVRgcSNFGGYLkRPQLNGTD--ADGWFD-------TGDLARIDADGYIRISGRSKDVIIRGGENIPVVEIE 458
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 532 SALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGvpESEELRkELISWVRKvlGPVAS---PDALHWAPALPKTRSGKI 608
Cdd:PRK13295 459 ALLYRHPAIAQVAIVAYPDERLGERACAFVVPRPG--QSLDFE-EMVEFLKA--QKVAKqyiPERLVVRDALPRTPSGKI 533
|
570
....*....|..
gi 446925704 609 MRRILRKIAANE 620
Cdd:PRK13295 534 QKFRLREMLRGE 545
|
|
| PRK09088 |
PRK09088 |
acyl-CoA synthetase; Validated |
107-621 |
4.69e-42 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181644 [Multi-domain] Cd Length: 488 Bit Score: 159.20 E-value: 4.69e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 107 SYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLvITADS 186
Cdd:PRK09088 24 TYAELDALVGRLAAVLRRRGCVDGERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSASELDALLQDAEPRL-LLGDD 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 187 SLRAGKLLPlkenVDLALalpgtecvenvivvyrnanpiemkpgrdlwyhlIIMEVDANCPP--EPMKAEDPLFILYTSG 264
Cdd:PRK09088 103 AVAAGRTDV----EDLAA---------------------------------FIASADALEPAdtPSIPPERVSLILFTSG 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 265 STGKPKGVLHTTGGyLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFEGVPQYPTWARLGhvv 344
Cdd:PRK09088 146 TSGQPKGVMLSERN-LQQTAHNFGVLGRVDAHSSFLCDAPMFHIIGLITSVRPVLAVGGSILVSNGFEPKRTLGRLG--- 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 345 DKHKVSILYTAPTAIRAMMREGDSYvresNRSSLRLLGSV---GEPINPEAWNWYynvVGEGrCPIVDTWWQTETGGILI 421
Cdd:PRK09088 222 DPALGITHYFCVPQMAQAFRAQPGF----DAAALRHLTALftgGAPHAAEDILGW---LDDG-IPMVDGFGMSEAGTVFG 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 422 APL-PGATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDswPGQMRTIWGDPDRFIEAYfsTFKNTYFTGDGA 500
Cdd:PRK09088 294 MSVdCDVIRAKAGAAGIPTPTVQTRVVDDQGNDCPAGVPGELLLRG--PNLSPGYWRRPQATARAF--TGDGWFRTGDIA 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 501 RRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQ-GICTFVTLQAGVPESEELRKELIS 579
Cdd:PRK09088 370 RRDADGFFWVVDRKKDMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQWGEvGYLAIVPADGAPLDLERIRSHLST 449
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 446925704 580 WVRKvlgpVASPDALHWAPALPKTRSGKIMRRILRKIAANEL 621
Cdd:PRK09088 450 RLAK----YKVPKHLRLVDALPRTASGKLQKARLRDALAAGR 487
|
|
| DltA |
cd05945 |
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ... |
84-613 |
7.58e-42 |
|
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341267 [Multi-domain] Cd Length: 449 Bit Score: 157.41 E-value: 7.58e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 84 LKEHPHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGG 163
Cdd:cd05945 1 AAANPDRPAVVEGGRT------LTYRELKERADALAAALASLGLDAGDPVVVYGHKSPDAIAAFLAALKAGHAYVPLDAS 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 164 FSPDSLASRIEDSQAKLVITAdsslragkllplkenvdlalalpgtecvenvivvyrnanpiemkpGRDLWYhliimevd 243
Cdd:cd05945 75 SPAERIREILDAAKPALLIAD---------------------------------------------GDDNAY-------- 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 244 ancppepmkaedplfILYTSGSTGKPKGVLHTTGGYLVYVASTFKEvFDLKQDDVYWCTAD-----------VGWITGHS 312
Cdd:cd05945 102 ---------------IIFTSGSTGRPKGVQISHDNLVSFTNWMLSD-FPLGPGDVFLNQAPfsfdlsvmdlyPALASGAT 165
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 313 YLIYGPLANGTTTLMFEGVPQyptwarlghvvdkHKVSILYTAPTAIR-AMMREGDSyvrESNRSSLRLLGSVGEPI-NP 390
Cdd:cd05945 166 LVPVPRDATADPKQLFRFLAE-------------HGITVWVSTPSFAAmCLLSPTFT---PESLPSLRHFLFCGEVLpHK 229
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 391 EAWNWyynvvgEGR---CPIVDTWWQTET----GGILIAPLPgATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLV 463
Cdd:cd05945 230 TARAL------QQRfpdARIYNTYGPTEAtvavTYIEVTPEV-LDGYDRLPIGYAKPGAKLVILDEDGRPVPPGEKGELV 302
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 464 IkdSWPGQMRTIWGDPDRFIEAYFSTFKNT-YFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAE 542
Cdd:cd05945 303 I--SGPSVSKGYLNNPEKTAAAFFPDEGQRaYRTGDLVRLEADGLLFYRGRLDFQVKLNGYRIELEEIEAALRQVPGVKE 380
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 446925704 543 AAVVGMPHDIKGQGICTFVTLQAGVPE--SEELRKELiswvRKVLGPVASPDALHWAPALPKTRSGKIMRRIL 613
Cdd:cd05945 381 AVVVPKYKGEKVTELIAFVVPKPGAEAglTKAIKAEL----AERLPPYMIPRRFVYLDELPLNANGKIDRKAL 449
|
|
| A_NRPS_Sfm_like |
cd12115 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ... |
82-613 |
1.28e-41 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.
Pssm-ID: 341280 [Multi-domain] Cd Length: 447 Bit Score: 156.71 E-value: 1.28e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 82 RHLKEHPHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVF 161
Cdd:cd12115 7 AQAARTPDAIALVCGDES------LTYAELNRRANRLAARLRAAGVGPESRVGVCLERTPDLVVALLAVLKAGAAYVPLD 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 162 GGFSPDSLASRIEDSQAKLVITadsslragkllplkenvdlalalpgtecvenvivvyrnanpiemkpgrdlwyhliime 241
Cdd:cd12115 81 PAYPPERLRFILEDAQARLVLT---------------------------------------------------------- 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 242 vdancppepmKAEDPLFILYTSGSTGKPKGVL---HTTGGYLVYVASTF-KEV-----------FDLKqddvywctadvg 306
Cdd:cd12115 103 ----------DPDDLAYVIYTSGSTGRPKGVAiehRNAAAFLQWAAAAFsAEElagvlastsicFDLS------------ 160
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 307 witghSYLIYGPLANGTTTLMFEGVPQYPTWARLGhvvdkhKVSILYTAPTAIRAMMREGDSyvresnRSSLRLLGSVGE 386
Cdd:cd12115 161 -----VFELFGPLATGGKVVLADNVLALPDLPAAA------EVTLINTVPSAAAELLRHDAL------PASVRVVNLAGE 223
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 387 PINPE-AWNWY--------YNVVGegrcPIVDTWWQTetggilIAPLPgATALKPGSATRPLFGIQPAIVDGEGNELEGA 457
Cdd:cd12115 224 PLPRDlVQRLYarlqvervVNLYG----PSEDTTYST------VAPVP-PGASGEVSIGRPLANTQAYVLDRALQPVPLG 292
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 458 AEGNLVIkdSWPGQMRTIWGDP----DRFIEAYFSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESA 533
Cdd:cd12115 293 VPGELYI--GGAGVARGYLGRPgltaERFLPDPFGPGARLYRTGDLVRWRPDGLLEFLGRADNQVKVRGFRIELGEIEAA 370
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 534 LVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGvpeSEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRIL 613
Cdd:cd12115 371 LRSIPGVREAVVVAIGDAAGERRLVAYIVAEPG---AAGLVEDLRRHLGTRLPAYMVPSRFVRLDALPLTPNGKIDRSAL 447
|
|
| PRK03640 |
PRK03640 |
o-succinylbenzoate--CoA ligase; |
88-616 |
1.43e-41 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 235146 [Multi-domain] Cd Length: 483 Bit Score: 157.43 E-value: 1.43e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 88 PHKPAIIWEGdhpsrhKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVhcVVF--GGFS 165
Cdd:PRK03640 16 PDRTAIEFEE------KKVTFMELHEAVVSVAGKLAALGVKKGDRVALLMKNGMEMILVIHALQQLGAV--AVLlnTRLS 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 166 PDSLASRIEDSQAKLVITADSslragklLPLKENVDLALALPgtecvENVIVVYRNANPIEMkpgrdlwYHLiimevdan 245
Cdd:PRK03640 88 REELLWQLDDAEVKCLITDDD-------FEAKLIPGISVKFA-----ELMNGPKEEAEIQEE-------FDL-------- 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 246 cppepmkaEDPLFILYTSGSTGKPKGVLHTTGGYLvYVASTFKEVFDLKQDDVYWCTADVGWITGHSYL----IYGPlan 321
Cdd:PRK03640 141 --------DEVATIMYTSGTTGKPKGVIQTYGNHW-WSAVGSALNLGLTEDDCWLAAVPIFHISGLSILmrsvIYGM--- 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 322 gTTTLM--FEGvpqyptwARLGHVVDKHKVSILYTAPTAIRAMMRE--GDSYvresnRSSLR--LLGsvGEPINP----- 390
Cdd:PRK03640 209 -RVVLVekFDA-------EKINKLLQTGGVTIISVVSTMLQRLLERlgEGTY-----PSSFRcmLLG--GGPAPKplleq 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 391 -EAWNwyynvvgegrCPIVDTWWQTETGGILIAPLPGATALKPGSATRPLFGIQPAIVDgEGNELEGAAEGNLVIKDswP 469
Cdd:PRK03640 274 cKEKG----------IPVYQSYGMTETASQIVTLSPEDALTKLGSAGKPLFPCELKIEK-DGVVVPPFEEGEIVVKG--P 340
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 470 GQMRTIWGDPDRFIEAyfstFKNTYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGM 548
Cdd:PRK03640 341 NVTKGYLNREDATRET----FQDGWFkTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPGVAEAGVVGV 416
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446925704 549 PHDIKGQGICTFVTLQAGVPESeelrkELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKI 616
Cdd:PRK03640 417 PDDKWGQVPVAFVVKSGEVTEE-----ELRHFCEEKLAKYKVPKRFYFVEELPRNASGKLLRHELKQL 479
|
|
| A_NRPS_Cytc1-like |
cd17643 |
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ... |
88-613 |
1.44e-41 |
|
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341298 [Multi-domain] Cd Length: 450 Bit Score: 156.70 E-value: 1.44e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 88 PHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPD 167
Cdd:cd17643 1 PEAVAVVDEDRR------LTYGELDARANRLARTLRAEGVGPGDRVALALPRSAELIVALLAILKAGGAYVPIDPAYPVE 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 168 SLASRIEDSQAKLVITadsslragkllplkenvdlalalpgtecvenvivvyrnanpiemkpgrdlwyhliimevdancp 247
Cdd:cd17643 75 RIAFILADSGPSLLLT---------------------------------------------------------------- 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 248 pepmKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVASTfKEVFDLKQDDVywctadvgWITGHSYL-------IYGPLA 320
Cdd:cd17643 91 ----DPDDLAYVIYTSGSTGRPKGVVVSHANVLALFAAT-QRWFGFNEDDV--------WTLFHSYAfdfsvweIWGALL 157
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 321 NGTTTLMfegVPQYP--TWARLGHVVDKHKVSILYTAPTAIRAMMREGDSyvRESNRSSLRLLGSVGEPINPEAWNWYYN 398
Cdd:cd17643 158 HGGRLVV---VPYEVarSPEDFARLLRDEGVTVLNQTPSAFYQLVEAADR--DGRDPLALRYVIFGGEALEAAMLRPWAG 232
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 399 VVGEGRCPIVDTWWQTETG------GILIAPLPGATALKPGsatRPLFGIQPAIVDGEGNELEGAAEGNLVIkdSWPGQM 472
Cdd:cd17643 233 RFGLDRPQLVNMYGITETTvhvtfrPLDAADLPAAAASPIG---RPLPGLRVYVLDADGRPVPPGVVGELYV--SGAGVA 307
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 473 RTIWGDP----DRFIEAYFSTF-KNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVG 547
Cdd:cd17643 308 RGYLGRPeltaERFVANPFGGPgSRMYRTGDLARRLPDGELEYLGRADEQVKIRGFRIELGEIEAALATHPSVRDAAVIV 387
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446925704 548 MPHDIKGQGICTFVTLQAGvpeSEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRIL 613
Cdd:cd17643 388 REDEPGDTRLVAYVVADDG---AAADIAELRALLKELLPDYMVPARYVPLDALPLTVNGKLDRAAL 450
|
|
| PLN03051 |
PLN03051 |
acyl-activating enzyme; Provisional |
137-621 |
1.59e-41 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215552 [Multi-domain] Cd Length: 499 Bit Score: 157.67 E-value: 1.59e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 137 MPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITADSSLRAGKLLPLKENVDLAlalpgTECVENVI 216
Cdd:PLN03051 1 MPMTVDAVIIYLAIVLAGCVVVSVADSFSAKEIATRLDISGAKGVFTQDVVLRGGRALPLYSKVVEA-----APAKAIVL 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 217 VVYRNANPIEMKPGRDLWYHLIIMEVDANCP------PEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVASTFKEV 290
Cdd:PLN03051 76 PAAGEPVAVPLREQDLSWCDFLGVAAAQGSVggneysPVYAPVESVTNILFSSGTTGEPKAIPWTHLSPLRCASDGWAHM 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 291 fDLKQDDVYWCTADVGWITGhSYLIYGPLANGTTTLMFEGVPQYPTWarlGHVVDKHKVSILYTAPTAIRAMMREGDSYV 370
Cdd:PLN03051 156 -DIQPGDVVCWPTNLGWMMG-PWLLYSAFLNGATLALYGGAPLGRGF---GKFVQDAGVTVLGLVPSIVKAWRHTGAFAM 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 371 RESNRSSLRLLGSVGEPINPEAWNWYYNVVGEGRcPIVDTWWQTETGG--ILIAPL-PGAtalkPGSATRPLFGIQPAIV 447
Cdd:PLN03051 231 EGLDWSKLRVFASTGEASAVDDVLWLSSVRGYYK-PVIEYCGGTELASgyISSTLLqPQA----PGAFSTASLGTRFVLL 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 448 DGEGNEL-EGAAEGNLVikdswpGQMRTIWGDPDRFIEA-----YFSTFKNTYFTGDGARRDED-------GYYWITGRV 514
Cdd:PLN03051 306 NDNGVPYpDDQPCVGEV------ALAPPMLGASDRLLNAdhdkvYYKGMPMYGSKGMPLRRHGDimkrtpgGYFCVQGRA 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 515 DDVLNVSGHRLGTAEIESALV-SHEAVAEAAVVGMPHDIKGQGICTFVtLQAGVPES-------EELRKELISWVRKVLG 586
Cdd:PLN03051 380 DDTMNLGGIKTSSVEIERACDrAVAGIAETAAVGVAPPDGGPELLVIF-LVLGEEKKgfdqarpEALQKKFQEAIQTNLN 458
|
490 500 510
....*....|....*....|....*....|....*
gi 446925704 587 PVASPDALHWAPALPKTRSGKIMRRILRKIAANEL 621
Cdd:PLN03051 459 PLFKVSRVKIVPELPRNASNKLLRRVLRDQLKKEL 493
|
|
| LC_FACS_like |
cd05935 |
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ... |
106-613 |
2.35e-41 |
|
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.
Pssm-ID: 341258 [Multi-domain] Cd Length: 430 Bit Score: 155.71 E-value: 2.35e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 106 VSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITAd 185
Cdd:cd05935 2 LTYLELLEVVKKLASFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVAVVG- 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 186 SSLRagkllplkenvDLALalpgtecvenvivvyrnanpiemkpgrdlwyhliimevdancppepmkaedplfILYTSGS 265
Cdd:cd05935 81 SELD-----------DLAL------------------------------------------------------IPYTSGT 95
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 266 TGKPKGVLHTTGGYLVYVASTfKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFEgvpqypTWAR--LGHV 343
Cdd:cd05935 96 TGLPKGCMHTHFSAAANALQS-AVWTGLTPSDVILACLPLFHVTGFVGSLNTAVYVGGTYVLMA------RWDRetALEL 168
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 344 VDKHKVSILYTAPTAIRAMMreGDSYVRESNRSSLRLLGSVGEPInPEAwnwyynvVGE---GRCPI--VDTWWQTETgg 418
Cdd:cd05935 169 IEKYKVTFWTNIPTMLVDLL--ATPEFKTRDLSSLKVLTGGGAPM-PPA-------VAEkllKLTGLrfVEGYGLTET-- 236
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 419 ilIAPLPGATALKPGSATR--PLFGIQPAIVDGE-GNELEGAAEGNLVIKDswPGQMRTIWGDPDRFIEAYFSTFKNTYF 495
Cdd:cd05935 237 --MSQTHTNPPLRPKLQCLgiP*FGVDARVIDIEtGRELPPNEVGEIVVRG--PQIFKGYWNRPEETEESFIEIKGRRFF 312
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 496 -TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVpESEELR 574
Cdd:cd05935 313 rTGDLGYMDEEGYFFFVDRVKRMINVSGFKVWPAEVEAKLYKHPAI*EVCVISVPDERVGEEVKAFIVLRPEY-RGKVTE 391
|
490 500 510
....*....|....*....|....*....|....*....
gi 446925704 575 KELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRIL 613
Cdd:cd05935 392 EDIIEWAREQMAAYKYPREVEFVDELPRSASGKILWRLL 430
|
|
| A_NRPS_Ta1_like |
cd12116 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ... |
102-613 |
4.56e-41 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.
Pssm-ID: 341281 [Multi-domain] Cd Length: 470 Bit Score: 155.91 E-value: 4.56e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 102 RHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLV 181
Cdd:cd12116 9 DDRSLSYAELDERANRLAARLRARGVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDYPADRLRYILEDAEPALV 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 182 ITADSSL-RAGKLLPLKENVDLALALPGTEcvenvivvyrnanpiemkpgrdlwyhliimevdancPPEPMKAEDPLFIL 260
Cdd:cd12116 89 LTDDALPdRLPAGLPVLLLALAAAAAAPAA------------------------------------PRTPVSPDDLAYVI 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 261 YTSGSTGKPKGVLHTTGGYLVYVAStFKEVFDLKQDDVYWCTADVGW-ItghSYL-IYGPLANGTTTLMFEGVPQYPTwA 338
Cdd:cd12116 133 YTSGSTGRPKGVVVSHRNLVNFLHS-MRERLGLGPGDRLLAVTTYAFdI---SLLeLLLPLLAGARVVIAPRETQRDP-E 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 339 RLGHVVDKHKVSILYTAPTAIRAMMREGdsyvrESNRSSLRLL-GsvGEPINP-----------EAWNWYynvvgeGrcP 406
Cdd:cd12116 208 ALARLIEAHSITVMQATPATWRMLLDAG-----WQGRAGLTALcG--GEALPPdlaarllsrvgSLWNLY------G--P 272
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 407 IVDTWWQTetggilIAPL-PGATALKPGsatRPLFGIQPAIVDGEGNELEGAAEGNLVIKDswPGQMRTIWGDPD----R 481
Cdd:cd12116 273 TETTIWST------AARVtAAAGPIPIG---RPLANTQVYVLDAALRPVPPGVPGELYIGG--DGVAQGYLGRPAltaeR 341
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 482 FIEAYFSTFKNT-YFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDiKGQGICTF 560
Cdd:cd12116 342 FVPDPFAGPGSRlYRTGDLVRRRADGRLEYLGRADGQVKIRGHRIELGEIEAALAAHPGVAQAAVVVREDG-GDRRLVAY 420
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 446925704 561 VTLQAGV-PESEELRKELiswvRKVLGPVASPDALHWAPALPKTRSGKIMRRIL 613
Cdd:cd12116 421 VVLKAGAaPDAAALRAHL----RATLPAYMVPSAFVRLDALPLTANGKLDRKAL 470
|
|
| PRK08276 |
PRK08276 |
long-chain-fatty-acid--CoA ligase; Validated |
106-614 |
2.72e-40 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236215 [Multi-domain] Cd Length: 502 Bit Score: 154.29 E-value: 2.72e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 106 VSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVIT-- 183
Cdd:PRK08276 12 VTYGELEARSNRLAHGLRALGLREGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDDSGAKVLIVsa 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 184 --ADSSLRAGKLLPLkeNVDLALALPGTecvenvivvyrnanpiemKPGRDLWYHLIimevdANCPPEPMKAEDP-LFIL 260
Cdd:PRK08276 92 alADTAAELAAELPA--GVPLLLVVAGP------------------VPGFRSYEEAL-----AAQPDTPIADETAgADML 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 261 YTSGSTGKPKGVL--------HTTGGYLVYVASTFkevFDLKQDDVYWCTAdvgwitghsyliygPLANgTTTLMFEGVP 332
Cdd:PRK08276 147 YSSGTTGRPKGIKrplpgldpDEAPGMMLALLGFG---MYGGPDSVYLSPA--------------PLYH-TAPLRFGMSA 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 333 QyptwaRLGHVV---------------DKHKVSILYTAPTAIRAMMREGDSyVRES-NRSSLRLLGSVGEPINPE----- 391
Cdd:PRK08276 209 L-----ALGGTVvvmekfdaeealaliERYRVTHSQLVPTMFVRMLKLPEE-VRARyDVSSLRVAIHAAAPCPVEvkram 282
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 392 -AWnWyynvvgeGrcPIVD-TWWQTETGGI-LIAPlpgATAL-KPGSATRPLFGiQPAIVDGEGNELEGAAEGNLVIKDs 467
Cdd:PRK08276 283 iDW-W-------G--PIIHeYYASSEGGGVtVITS---EDWLaHPGSVGKAVLG-EVRILDEDGNELPPGEIGTVYFEM- 347
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 468 wPGQMRTIWGDPDRFIEAYFstfKNTYFT-GDGARRDEDGYYWITGRVDDVLnVSGhrlGT----AEIESALVSHEAVAE 542
Cdd:PRK08276 348 -DGYPFEYHNDPEKTAAARN---PHGWVTvGDVGYLDEDGYLYLTDRKSDMI-ISG---GVniypQEIENLLVTHPKVAD 419
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 446925704 543 AAVVGMPHDIKGQGICTFVTLQAGVPESEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILR 614
Cdd:PRK08276 420 VAVFGVPDEEMGERVKAVVQPADGADAGDALAAELIAWLRGRLAHYKCPRSIDFEDELPRTPTGKLYKRRLR 491
|
|
| OSB_CoA_lg |
cd05912 |
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ... |
106-615 |
8.93e-40 |
|
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.
Pssm-ID: 341238 [Multi-domain] Cd Length: 411 Bit Score: 150.96 E-value: 8.93e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 106 VSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLvitad 185
Cdd:cd05912 2 YTFAELFEEVSRLAEHLAALGVRKGDRVALLSKNSIEMILLIHALWLLGAEAVLLNTRLTPNELAFQLKDSDVKL----- 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 186 sslragkllplkenvdlalalpgtecvenvivvyrnanpiemkpgrdlwyhliimevdancppepmkaEDPLFILYTSGS 265
Cdd:cd05912 77 --------------------------------------------------------------------DDIATIMYTSGT 88
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 266 TGKPKGVLHTTGGYLVYVASTfKEVFDLKQDDVYWCTADVGWITGHSYLIYGpLANGTTTLMFEGVPQyptwARLGHVVD 345
Cdd:cd05912 89 TGKPKGVQQTFGNHWWSAIGS-ALNLGLTEDDNWLCALPLFHISGLSILMRS-VIYGMTVYLVDKFDA----EQVLHLIN 162
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 346 KHKVSILYTAPTAIRAMMREGDsyvrESNRSSLR--LLGsvGEPINPEAwnwyYNVVGEGRCPIVDTWWQTETGGILIAP 423
Cdd:cd05912 163 SGKVTIISVVPTMLQRLLEILG----EGYPNNLRciLLG--GGPAPKPL----LEQCKEKGIPVYQSYGMTETCSQIVTL 232
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 424 LPGATALKPGSATRPLFGIQPAIVDGEGNELEgaaEGNLVIKDswPGQMRTIWGDPDRFIEayfsTFKNTYF-TGDGARR 502
Cdd:cd05912 233 SPEDALNKIGSAGKPLFPVELKIEDDGQPPYE---VGEILLKG--PNVTKGYLNRPDATEE----SFENGWFkTGDIGYL 303
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 503 DEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVpeSEElrkELISWVR 582
Cdd:cd05912 304 DEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPAIKEAGVVGIPDDKWGQVPVAFVVSERPI--SEE---ELIAYCS 378
|
490 500 510
....*....|....*....|....*....|...
gi 446925704 583 KVLGPVASPDALHWAPALPKTRSGKIMRRILRK 615
Cdd:cd05912 379 EKLAKYKVPKKIYFVDELPRTASGKLLRHELKQ 411
|
|
| menE |
TIGR01923 |
O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, ... |
107-610 |
5.48e-39 |
|
O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, which is involved in the fourth step of the menaquinone biosynthesis pathway. O-succinylbenzoate-CoA ligase, together with menB - naphtoate synthase, take 2-succinylbenzoate and convert it into 1,4-di-hydroxy-2- naphtoate. [Biosynthesis of cofactors, prosthetic groups, and carriers, Menaquinone and ubiquinone]
Pssm-ID: 162605 [Multi-domain] Cd Length: 436 Bit Score: 149.14 E-value: 5.48e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 107 SYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITADs 186
Cdd:TIGR01923 1 TWQDLDCEAAHLAKALKAQGIRSGSRVALVGQNSIEMVLLLHACLLLGAEIAMLNTRLTENERTNQLEDLDVQLLLTDS- 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 187 slragkllPLKENVDLALALPGTEcvenvivvYRNANPIEMKPgrdlwyhliimevdancppePMKAEDPLFILYTSGST 266
Cdd:TIGR01923 80 --------LLEEKDFQADSLDRIE--------AAGRYETSLSA--------------------SFNMDQIATLMFTSGTT 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 267 GKPKGVLHTTGGYLVYVASTfKEVFDLKQDDVYWCTADVGWITGHSyLIYGPLANGTTTLMFEGVPQyptwarLGHVVDK 346
Cdd:TIGR01923 124 GKPKAVPHTFRNHYASAVGS-KENLGFTEDDNWLLSLPLYHISGLS-ILFRWLIEGATLRIVDKFNQ------LLEMIAN 195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 347 HKVSILYTAPTAIRAMMREGDsyvRESNRSSLRLLGS-VGEPINPEAwnwyynvvGEGRCPIVDTWWQTETGGILIAPLP 425
Cdd:TIGR01923 196 ERVTHISLVPTQLNRLLDEGG---HNENLRKILLGGSaIPAPLIEEA--------QQYGLPIYLSYGMTETCSQVTTATP 264
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 426 GATALKPGSAtRPLFGIQPAI-VDGEgnelegAAEGNLVIKDswPGQMRTIWgDPDRFIEAyfsTFKNTYF-TGDGARRD 503
Cdd:TIGR01923 265 EMLHARPDVG-RPLAGREIKIkVDNK------EGHGEIMVKG--ANLMKGYL-YQGELTPA---FEQQGWFnTGDIGELD 331
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 504 EDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVtlqagVPESEELRKELISWVRK 583
Cdd:TIGR01923 332 GEGFLYVLGRRDDLIISGGENIYPEEIETVLYQHPGIQEAVVVPKPDAEWGQVPVAYI-----VSESDISQAKLIAYLTE 406
|
490 500
....*....|....*....|....*..
gi 446925704 584 VLGPVASPDALHWAPALPKTRSGKIMR 610
Cdd:TIGR01923 407 KLAKYKVPIAFEKLDELPYNASGKILR 433
|
|
| PRK06178 |
PRK06178 |
acyl-CoA synthetase; Validated |
85-608 |
2.21e-38 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235724 [Multi-domain] Cd Length: 567 Bit Score: 149.81 E-value: 2.21e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 85 KEHPHKPAIIWEGdhpsrhKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGF 164
Cdd:PRK06178 44 RERPQRPAIIFYG------HVITYAELDELSDRFAALLRQRGVGAGDRVAVFLPNCPQFHIVFFGILKLGAVHVPVSPLF 117
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 165 SPDSLASRIEDSQAKLVITADSslragkLLPLKENVdlalaLPGTEcVENVIVV----YRNANPIEMKPGR--------- 231
Cdd:PRK06178 118 REHELSYELNDAGAEVLLALDQ------LAPVVEQV-----RAETS-LRHVIVTsladVLPAEPTLPLPDSlraprlaaa 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 232 ---DLWYHLIIMEVDAncPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGgYLVYVASTFKEV-FDLKQDDVYWCTADVGW 307
Cdd:PRK06178 186 gaiDLLPALRACTAPV--PLPPPALDALAALNYTGGTTGMPKGCEHTQR-DMVYTAAAAYAVaVVGGEDSVFLSFLPEFW 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 308 ITGHSYLIYGPLANGTTTLMFEgvpqypTWARLG--HVVDKHKVSILY-TAPTAIRAMMREGdsyVRESNRSSLRLLGSV 384
Cdd:PRK06178 263 IAGENFGLLFPLFSGATLVLLA------RWDAVAfmAAVERYRVTRTVmLVDNAVELMDHPR---FAEYDLSSLRQVRVV 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 385 G--EPINPEAwnwyynvvgEGRcpivdtwWQTETGGILIAPLPGATA-----------------LKpgsaTRPLF----- 440
Cdd:PRK06178 334 SfvKKLNPDY---------RQR-------WRALTGSVLAEAAWGMTEthtcdtftagfqdddfdLL----SQPVFvglpv 393
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 441 -GIQPAIVDGE-GNELEGAAEGNLVIKDswPGQMRTIWGDPdrfiEAYFSTFKNTYF-TGDGARRDEDGYYWITGRVDDV 517
Cdd:PRK06178 394 pGTEFKICDFEtGELLPLGAEGEIVVRT--PSLLKGYWNKP----EATAEALRDGWLhTGDIGKIDEQGFLHYLGRRKEM 467
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 518 LNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEElrkELISWVRKVLGPVASPDaLHWA 597
Cdd:PRK06178 468 LKVNGMSVFPSEVEALLGQHPAVLGSAVVGRPDPDKGQVPVAFVQLKPGADLTAA---ALQAWCRENMAVYKVPE-IRIV 543
|
570
....*....|.
gi 446925704 598 PALPKTRSGKI 608
Cdd:PRK06178 544 DALPMTATGKV 554
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
81-622 |
6.54e-37 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 148.77 E-value: 6.54e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 81 DRHLKEHPHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVV 160
Cdd:PRK12467 519 EAQARQHPERPALVFGEQV------LSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMVVGLLAVLKAGGAYVPL 592
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 161 FGGFSPDSLASRIEDSQAKLVITaDSSLRAgkLLPLKENVD-LALALPGtecvenvivvyrnanpiemkpgrDLWYHlii 239
Cdd:PRK12467 593 DPEYPQDRLAYMLDDSGVRLLLT-QSHLLA--QLPVPAGLRsLCLDEPA-----------------------DLLCG--- 643
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 240 meVDANCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVASTfKEVFDLKQDDVYWCTADVGWITGHSYLiYGPL 319
Cdd:PRK12467 644 --YSGHNPEVALDPDNLAYVIYTSGSTGQPKGVAISHGALANYVCVI-AERLQLAADDSMLMVSTFAFDLGVTEL-FGAL 719
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 320 ANGTTTLMfegVPQYPTW--ARLGHVVDKHKVSILYTAPTAIRAMMRegDSYVRESNRSSLRLLGsvGEPINPEAWNWYY 397
Cdd:PRK12467 720 ASGATLHL---LPPDCARdaEAFAALMADQGVTVLKIVPSHLQALLQ--ASRVALPRPQRALVCG--GEALQVDLLARVR 792
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 398 NVVGEGRcpIVDTWWQTETG-GILIAPLPGATALKPGSAT-RPLFGIQPAIVDGEGNELEGAAEGNLVIkdSWPGQMRTI 475
Cdd:PRK12467 793 ALGPGAR--LINHYGPTETTvGVSTYELSDEERDFGNVPIgQPLANLGLYILDHYLNPVPVGVVGELYI--GGAGLARGY 868
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 476 WGDP----DRFIEAYFSTF-KNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPH 550
Cdd:PRK12467 869 HRRPaltaERFVPDPFGADgGRLYRTGDLARYRADGVIEYLGRMDHQVKIRGFRIELGEIEARLLAQPGVREAVVLAQPG 948
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 446925704 551 DIKGQGICTFV-TLQAGVPESEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKIAANELD 622
Cdd:PRK12467 949 DAGLQLVAYLVpAAVADGAEHQATRDELKAQLRQVLPDYMVPAHLLLLDSLPLTPNGKLDRKALPKPDASAVQ 1021
|
|
| FACL_like_6 |
cd05922 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
115-614 |
8.74e-37 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341246 [Multi-domain] Cd Length: 457 Bit Score: 143.35 E-value: 8.74e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 115 VCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDslasrIEDSQAKLVItADSSLRagkll 194
Cdd:cd05922 3 VSAAASALLEAGGVRGERVVLILPNRFTYIELSFAVAYAGGRLGLVFVPLNPT-----LKESVLRYLV-ADAGGR----- 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 195 plkenvdLALALPG-TECVENVIVVYRNanPIEMKPGRDLWyhliimEVDANCPPEPMKAEDPLFILYTSGSTGKPKGVL 273
Cdd:cd05922 72 -------IVLADAGaADRLRDALPASPD--PGTVLDADGIR------AARASAPAHEVSHEDLALLLYTSGSTGSPKLVR 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 274 --HTTggyLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFEGVPQYPTWARLGHvvdkHKVSI 351
Cdd:cd05922 137 lsHQN---LLANARSIAEYLGITADDRALTVLPLSYDYGLSVLNTHLLRGATLVLTNDGVLDDAFWEDLRE----HGATG 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 352 LYTAPTAIRAMMREGdsyVRESNRSSLRLLGSVGEPInPEAWNWYYNVVGEGrCPIVDTWWQTE-TGGILIAPlPGATAL 430
Cdd:cd05922 210 LAGVPSTYAMLTRLG---FDPAKLPSLRYLTQAGGRL-PQETIARLRELLPG-AQVYVMYGQTEaTRRMTYLP-PERILE 283
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 431 KPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIkdSWPGQMRTIWGDPDRFIEAyfSTFKNTYFTGDGARRDEDGYYWI 510
Cdd:cd05922 284 KPGSIGLAIPGGEFEILDDDGTPTPPGEPGEIVH--RGPNVMKGYWNDPPYRRKE--GRGGGVLHTGDLARRDEDGFLFI 359
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 511 TGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPhDIKGQGICTFVTLQAGVPESEELRKeliswVRKVLGPVAS 590
Cdd:cd05922 360 VGRRDRMIKLFGNRISPTEIEAAARSIGLIIEAAAVGLP-DPLGEKLALFVTAPDKIDPKDVLRS-----LAERLPPYKV 433
|
490 500
....*....|....*....|....
gi 446925704 591 PDALHWAPALPKTRSGKIMRRILR 614
Cdd:cd05922 434 PATVRVVDELPLTASGKVDYAALR 457
|
|
| 23DHB-AMP_lg |
cd05920 |
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ... |
80-613 |
1.15e-36 |
|
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.
Pssm-ID: 341244 [Multi-domain] Cd Length: 482 Bit Score: 143.62 E-value: 1.15e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIwegDHPSRHkivSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCV 159
Cdd:cd05920 21 LARSAARHPDRIAVV---DGDRRL---TYRELDRRADRLAAGLRGLGIRPGDRVVVQLPNVAEFVVLFFALLRLGAVPVL 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFGGFSPDSLASRIEDSQAKLVITADsslragkllplkenvdlalalpgtecvENVIVVYRNanpiemkpgrdlwyhlII 239
Cdd:cd05920 95 ALPSHRRSELSAFCAHAEAVAYIVPD---------------------------RHAGFDHRA----------------LA 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 240 MEVDANCPpepmkaeDPLFILYTSGSTGKPKGVLHTTGGYlVYVASTFKEVFDLKQDDVYWCTADVGwitgHSYLIYGPL 319
Cdd:cd05920 132 RELAESIP-------EVALFLLSGGTTGTPKLIPRTHNDY-AYNVRASAEVCGLDQDTVYLAVLPAA----HNFPLACPG 199
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 320 ANGTttLMFEGVPQYPTWARLGHV---VDKHKVSILYTAPTAIRAMMREGDSyvRESNRSSLRLLGSVGEPINPEAWNWY 396
Cdd:cd05920 200 VLGT--LLAGGRVVLAPDPSPDAAfplIEREGVTVTALVPALVSLWLDAAAS--RRADLSSLRLLQVGGARLSPALARRV 275
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 397 YNVVGegrcPIVDTWWQTETGGILIAPLPGATALKPGSATRPLF-GIQPAIVDGEGNELEGAAEGNLVIKDswPGQMRTI 475
Cdd:cd05920 276 PPVLG----CTLQQVFGMAEGLLNYTRLDDPDEVIIHTQGRPMSpDDEIRVVDEEGNPVPPGEEGELLTRG--PYTIRGY 349
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 476 WGDPDRFIEAYfsTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQ 555
Cdd:cd05920 350 YRAPEHNARAF--TPDGFYRTGDLVRRTPDGYLVVEGRIKDQINRGGEKIAAEEVENLLLRHPAVHDAAVVAMPDELLGE 427
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 446925704 556 GICTFVTLQAGVPESEELRKELiswvrKVLGpVAS---PDALHWAPALPKTRSGKIMRRIL 613
Cdd:cd05920 428 RSCAFVVLRDPPPSAAQLRRFL-----RERG-LAAyklPDRIEFVDSLPLTAVGKIDKKAL 482
|
|
| PRK06710 |
PRK06710 |
long-chain-fatty-acid--CoA ligase; Validated |
80-613 |
1.21e-36 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 180666 [Multi-domain] Cd Length: 563 Bit Score: 144.79 E-value: 1.21e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIWEGdhpsrhKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCV 159
Cdd:PRK06710 30 VEQMASRYPEKKALHFLG------KDITFSVFHDKVKRFANYLQKLGVEKGDRVAIMLPNCPQAVIGYYGTLLAGGIVVQ 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFGGFSPDSLASRIEDSQAKLVITADSSL------------------RAGKLLPLKENvdlaLALPGTECVE-NVIVVYR 220
Cdd:PRK06710 104 TNPLYTERELEYQLHDSGAKVILCLDLVFprvtnvqsatkiehvivtRIADFLPFPKN----LLYPFVQKKQsNLVVKVS 179
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 221 NANPIEmkpgrdLWYHL---IIMEVDANCPPEpmkaEDPLFILYTSGSTGKPKGVLHTtggYLVYVASTFKEVFDLkqdd 297
Cdd:PRK06710 180 ESETIH------LWNSVekeVNTGVEVPCDPE----NDLALLQYTGGTTGFPKGVMLT---HKNLVSNTLMGVQWL---- 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 298 vYWCTADVGWITG--HSYLIYGPLANGTTTLMfEG-----VPQYPTwARLGHVVDKHKVSILYTAPTAIRAMMREgdSYV 370
Cdd:PRK06710 243 -YNCKEGEEVVLGvlPFFHVYGMTAVMNLSIM-QGykmvlIPKFDM-KMVFEAIKKHKVTLFPGAPTIYIALLNS--PLL 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 371 RESNRSSLRLLGSVGEPINPEAWNWYYNVVGeGRcpIVDTWWQTETggiliAPLPGATAL----KPGSATRPLFGIQPAI 446
Cdd:PRK06710 318 KEYDISSIRACISGSAPLPVEVQEKFETVTG-GK--LVEGYGLTES-----SPVTHSNFLwekrVPGSIGVPWPDTEAMI 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 447 VDGEGNELEGAAE-GNLVIKDswPGQMRTIWGDPdrfiEAYFSTFKNTYF-TGDGARRDEDGYYWITGRVDDVLNVSGHR 524
Cdd:PRK06710 390 MSLETGEALPPGEiGEIVVKG--PQIMKGYWNKP----EETAAVLQDGWLhTGDVGYMDEDGFFYVKDRKKDMIVASGFN 463
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 525 LGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEElrkELISWVRKVLGPVASPDALHWAPALPKTR 604
Cdd:PRK06710 464 VYPREVEEVLYEHEKVQEVVTIGVPDPYRGETVKAFVVLKEGTECSEE---ELNQFARKYLAAYKVPKVYEFRDELPKTT 540
|
....*....
gi 446925704 605 SGKIMRRIL 613
Cdd:PRK06710 541 VGKILRRVL 549
|
|
| PRK07470 |
PRK07470 |
acyl-CoA synthetase; Validated |
77-615 |
3.46e-36 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 180988 [Multi-domain] Cd Length: 528 Bit Score: 142.87 E-value: 3.46e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 77 ANCLDRHLKEHPHKPAIIWeGDhpsrhKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAV 156
Cdd:PRK07470 10 AHFLRQAARRFPDRIALVW-GD-----RSWTWREIDARVDALAAALAARGVRKGDRILVHSRNCNQMFESMFAAFRLGAV 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 157 HCVVFGGFSPDSLASRIEDSQAKLVITADSslragkllpLKENVDLALAlpGTECVENVIVvyrnanpIEMKPGRDLWYH 236
Cdd:PRK07470 84 WVPTNFRQTPDEVAYLAEASGARAMICHAD---------FPEHAAAVRA--ASPDLTHVVA-------IGGARAGLDYEA 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 237 LIIMEVDANCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVASTFKEVF-DLKQDDVYWCTADVGWITGHSYLI 315
Cdd:PRK07470 146 LVARHLGARVANAAVDHDDPCWFFFTSGTTGRPKAAVLTHGQMAFVITNHLADLMpGTTEQDASLVVAPLSHGAGIHQLC 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 316 ygPLANGTTTLMFEGVPQYPT--WArlghVVDKHKVSILYTAPTaIRAMMREgDSYVRESNRSSLRLLGSVGEPINPEAW 393
Cdd:PRK07470 226 --QVARGAATVLLPSERFDPAevWA----LVERHRVTNLFTVPT-ILKMLVE-HPAVDRYDHSSLRYVIYAGAPMYRADQ 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 394 NWYYNVVGEgrcPIVDTWWQTE-TGGILIAPLPG-----ATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDs 467
Cdd:PRK07470 298 KRALAKLGK---VLVQYFGLGEvTGNITVLPPALhdaedGPDARIGTCGFERTGMEVQIQDDEGRELPPGETGEICVIG- 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 468 wPGQMRTIWGDPdrfiEAYFSTFKNTYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVV 546
Cdd:PRK07470 374 -PAVFAGYYNNP----EANAKAFRDGWFrTGDLGHLDARGFLYITGRASDMYISGGSNVYPREIEEKLLTHPAVSEVAVL 448
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 547 GMPHDIKGQ-GICTFVtLQAGVPESEElrkELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRK 615
Cdd:PRK07470 449 GVPDPVWGEvGVAVCV-ARDGAPVDEA---ELLAWLDGKVARYKLPKRFFFWDALPKSGYGKITKKMVRE 514
|
|
| PRK13391 |
PRK13391 |
acyl-CoA synthetase; Provisional |
81-614 |
5.36e-36 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 184022 [Multi-domain] Cd Length: 511 Bit Score: 142.14 E-value: 5.36e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 81 DRHLKEHPHKPAIIwegdHPSRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVV 160
Cdd:PRK13391 4 GIHAQTTPDKPAVI----MASTGEVVTYRELDERSNRLAHLFRSLGLKRGDHVAIFMENNLRYLEVCWAAERSGLYYTCV 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 161 FGGFSPDSLASRIEDSQAKLVIT----ADSSLRAGKLLP---LKENVDLALALPGTECVENVIvvyrnanpiemkpgrdl 233
Cdd:PRK13391 80 NSHLTPAEAAYIVDDSGARALITsaakLDVARALLKQCPgvrHRLVLDGDGELEGFVGYAEAV----------------- 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 234 wyhliimevdANCPPEPMKAE---DPLfiLYTSGSTGKPKGVL---------HTTGgylvyVASTFKEVFDLKQDDVYWC 301
Cdd:PRK13391 143 ----------AGLPATPIADEslgTDM--LYSSGTTGRPKGIKrplpeqppdTPLP-----LTAFLQRLWGFRSDMVYLS 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 302 TADVGwitgHSyliyGPLA--------NGTTTLMFEGVPQypTWARLghvVDKHKVSILYTAPTAIRAMMREGDSyVRES 373
Cdd:PRK13391 206 PAPLY----HS----APQRavmlvirlGGTVIVMEHFDAE--QYLAL---IEEYGVTHTQLVPTMFSRMLKLPEE-VRDK 271
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 374 -NRSSLRLLGSVGEPINPE------AWnWyynvvgegrCPIVDTWWQTETGGiliaplpGATAL-------KPGSATRPL 439
Cdd:PRK13391 272 yDLSSLEVAIHAAAPCPPQvkeqmiDW-W---------GPIIHEYYAATEGL-------GFTACdseewlaHPGTVGRAM 334
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 440 FGIqPAIVDGEGNELEgaaegnlvikdswPGQMRTIW----------GDPDRFIEAY-----FSTfkntyfTGDGARRDE 504
Cdd:PRK13391 335 FGD-LHILDDDGAELP-------------PGEPGTIWfeggrpfeylNDPAKTAEARhpdgtWST------VGDIGYVDE 394
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 505 DGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEELRKELISWVRKV 584
Cdd:PRK13391 395 DGYLYLTDRAAFMIISGGVNIYPQEAENLLITHPKVADAAVFGVPNEDLGEEVKAVVQPVDGVDPGPALAAELIAFCRQR 474
|
570 580 590
....*....|....*....|....*....|
gi 446925704 585 LGPVASPDALHWAPALPKTRSGKIMRRILR 614
Cdd:PRK13391 475 LSRQKCPRSIDFEDELPRLPTGKLYKRLLR 504
|
|
| PRK06164 |
PRK06164 |
acyl-CoA synthetase; Validated |
77-622 |
6.21e-36 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235722 [Multi-domain] Cd Length: 540 Bit Score: 142.19 E-value: 6.21e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 77 ANCLDRHLKEHPHKPAIIWEGdhpsrhKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAV 156
Cdd:PRK06164 13 ASLLDAHARARPDAVALIDED------RPLSRAELRALVDRLAAWLAAQGVRRGDRVAVWLPNCIEWVVLFLACARLGAT 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 157 HCVVFGGFSPDSLASRIEDSQAKLVITADsSLRAGKLLPLKENVDLALALPgtecVENVIVVyRNANPIEMKPGRDLWYH 236
Cdd:PRK06164 87 VIAVNTRYRSHEVAHILGRGRARWLVVWP-GFKGIDFAAILAAVPPDALPP----LRAIAVV-DDAADATPAPAPGARVQ 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 237 LIIMEVDANCP--PEPMKAEDPLFILY-TSGSTGKPKGVLHTTGGyLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSY 313
Cdd:PRK06164 161 LFALPDPAPPAaaGERAADPDAGALLFtTSGTTSGPKLVLHRQAT-LLRHARAIARAYGYDPGAVLLAALPFCGVFGFST 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 314 LIyGPLANGTTTLMFEGVPQYPTWARLGhvvdKHKVSILYTAPTAIRAMMREGDsyvRESNRSSLRLLGSVGepINPEAW 393
Cdd:PRK06164 240 LL-GALAGGAPLVCEPVFDAARTARALR----RHRVTHTFGNDEMLRRILDTAG---ERADFPSARLFGFAS--FAPALG 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 394 NWYYNVVGEGrCPIVDTWWQTEtggiLIAPLPGATALKPGSAtRPLFGIQPA-------IVDGEGNE-LEGAAEGNLVIK 465
Cdd:PRK06164 310 ELAALARARG-VPLTGLYGSSE----VQALVALQPATDPVSV-RIEGGGRPAspearvrARDPQDGAlLPDGESGEIEIR 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 466 DswPGQMRTIWGDPDRFIEAYFStfkNTYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAA 544
Cdd:PRK06164 384 A--PSLMRGYLDNPDATARALTD---DGYFrTGDLGYTRGDGQFVYQTRMGDSLRLGGFLVNPAEIEHALEALPGVAAAQ 458
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 545 VVGMPHDikGQGIC-TFVTLQAGVPESEElrkELISWVRKVLGPVASPDALHWAPALPKTRSG---KIMRRILRKIAANE 620
Cdd:PRK06164 459 VVGATRD--GKTVPvAFVIPTDGASPDEA---GLMAACREALAGFKVPARVQVVEAFPVTESAngaKIQKHRLREMAQAR 533
|
..
gi 446925704 621 LD 622
Cdd:PRK06164 534 LA 535
|
|
| caiC |
PRK08008 |
putative crotonobetaine/carnitine-CoA ligase; Validated |
87-614 |
8.67e-36 |
|
putative crotonobetaine/carnitine-CoA ligase; Validated
Pssm-ID: 181195 [Multi-domain] Cd Length: 517 Bit Score: 141.36 E-value: 8.67e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 87 HPHKPAIIWEgdhpSRHKIV---SYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGG 163
Cdd:PRK08008 20 YGHKTALIFE----SSGGVVrrySYLELNEEINRTANLFYSLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINAR 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 164 FSPDSLASRIEDSQAKLVITADsslragKLLPLKEnvdlALALPGTECVENvIVVYRNANPIEmkPGRDLWYHLIIMEVD 243
Cdd:PRK08008 96 LLREESAWILQNSQASLLVTSA------QFYPMYR----QIQQEDATPLRH-ICLTRVALPAD--DGVSSFTQLKAQQPA 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 244 ANCPPEPMKAEDPLFILYTSGSTGKPKGVLHTT-----GGYlvYVASTFKevfdLKQDDVYW-----------CTADVGW 307
Cdd:PRK08008 163 TLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHynlrfAGY--YSAWQCA----LRDDDVYLtvmpafhidcqCTAAMAA 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 308 ITGhsyliygplanGTTTLMFEGVPQYPTWARlghvVDKHKVSILYTAPTAIRAMM-------------REGDSYVRESN 374
Cdd:PRK08008 237 FSA-----------GATFVLLEKYSARAFWGQ----VCKYRATITECIPMMIRTLMvqppsandrqhclREVMFYLNLSD 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 375 --------RSSLRLLGSVGepinpeawnwyynvvgegrcpivdtwwQTETGGILIAPLPGATALKPgSATRPLFGIQPAI 446
Cdd:PRK08008 302 qekdafeeRFGVRLLTSYG---------------------------MTETIVGIIGDRPGDKRRWP-SIGRPGFCYEAEI 353
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 447 VDGEGNELEGAAEGNLVIKDSwPGqmRTI----WGDPdrfiEAYFSTFKNT--YFTGDGARRDEDGYYWITGRVDDVLNV 520
Cdd:PRK08008 354 RDDHNRPLPAGEIGEICIKGV-PG--KTIfkeyYLDP----KATAKVLEADgwLHTGDTGYVDEEGFFYFVDRRCNMIKR 426
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 521 SGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEElrkELISWVRKVLGPVASPDALHWAPAL 600
Cdd:PRK08008 427 GGENVSCVELENIIATHPKIQDIVVVGIKDSIRDEAIKAFVVLNEGETLSEE---EFFAFCEQNMAKFKVPSYLEIRKDL 503
|
570
....*....|....
gi 446925704 601 PKTRSGKIMRRILR 614
Cdd:PRK08008 504 PRNCSGKIIKKNLK 517
|
|
| PRK07788 |
PRK07788 |
acyl-CoA synthetase; Validated |
86-616 |
9.39e-35 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236097 [Multi-domain] Cd Length: 549 Bit Score: 138.91 E-value: 9.39e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 86 EHPHKPAIIWEgdhpsrHKIVSYKELHDEVCRFANVLKKYGIGKGDRV-VL---YMPMVteaaIAMLACARIGAVHCVVF 161
Cdd:PRK07788 61 RAPDRAALIDE------RGTLTYAELDEQSNALARGLLALGVRAGDGVaVLarnHRGFV----LALYAAGKVGARIILLN 130
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 162 GGFSPDSLASRIEDSQAKLVItADSSLrAGKLLPLKENVDLALALPGtecvenvivvyrnaNPIEMKPGRDLWYHL--II 239
Cdd:PRK07788 131 TGFSGPQLAEVAAREGVKALV-YDDEF-TDLLSALPPDLGRLRAWGG--------------NPDDDEPSGSTDETLddLI 194
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 240 MEVDANCPPEPMKAEDplFILYTSGSTGKPKGVLHTTGGYLVYVASTFKEVfDLKQDDVYWCTADVGWITGHSYLIYGpL 319
Cdd:PRK07788 195 AGSSTAPLPKPPKPGG--IVILTSGTTGTPKGAPRPEPSPLAPLAGLLSRV-PFRAGETTLLPAPMFHATGWAHLTLA-M 270
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 320 ANGTTTLM---FEgvpqyPtwARLGHVVDKHKVSILYTAPTAIRAMMREGDSYVRESNRSSLRLLGSVGEPINPEAWNWY 396
Cdd:PRK07788 271 ALGSTVVLrrrFD-----P--EATLEDIAKHKATALVVVPVMLSRILDLGPEVLAKYDTSSLKIIFVSGSALSPELATRA 343
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 397 --------YNVVGegrcpivdtwwQTETGGILIAPlPGATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDSW 468
Cdd:PRK07788 344 leafgpvlYNLYG-----------STEVAFATIAT-PEDLAEAPGTVGRPPKGVTVKILDENGNEVPRGVVGRIFVGNGF 411
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 469 PGQMRTIWGDPDRfIEAYFSTfkntyftGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGM 548
Cdd:PRK07788 412 PFEGYTDGRDKQI-IDGLLSS-------GDVGYFDEDGLLFVDGRDDDMIVSGGENVFPAEVEDLLAGHPDVVEAAVIGV 483
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446925704 549 PHDIKGQGICTFVTLQAGVPESEElrkELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKI 616
Cdd:PRK07788 484 DDEEFGQRLRAFVVKAPGAALDED---AIKDYVRDNLARYKVPRDVVFLDELPRNPTGKVLKRELREM 548
|
|
| PRK12583 |
PRK12583 |
acyl-CoA synthetase; Provisional |
80-621 |
2.37e-34 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237145 [Multi-domain] Cd Length: 558 Bit Score: 137.98 E-value: 2.37e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIwegdhpSRHKIV--SYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVH 157
Cdd:PRK12583 24 FDATVARFPDREALV------VRHQALryTWRQLADAVDRLARGLLALGVQPGDRVGIWAPNCAEWLLTQFATARIGAIL 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 158 CVVFGGFSPDSLASRIEDSQAKLVITAD----SSLRA--GKLLP-LKENVDLALALPGTECVENViVVYRNANPiemkPG 230
Cdd:PRK12583 98 VNINPAYRASELEYALGQSGVRWVICADafktSDYHAmlQELLPgLAEGQPGALACERLPELRGV-VSLAPAPP----PG 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 231 RDLWYHLI-----IMEVDANCPPEPMKAEDPLFILYTSGSTGKPKGVLHT-----TGGYLVyvastfKEVFDLKQDDV-- 298
Cdd:PRK12583 173 FLAWHELQargetVSREALAERQASLDRDDPINIQYTSGTTGFPKGATLShhnilNNGYFV------AESLGLTEHDRlc 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 299 -----YWC----TADVGWITGHSYLIY-----GPLAngttTLmfegvpqyptwarlgHVVDKHKVSILYTAPTAIRAMMR 364
Cdd:PRK12583 247 vpvplYHCfgmvLANLGCMTVGACLVYpneafDPLA----TL---------------QAVEEERCTALYGVPTMFIAELD 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 365 EGDSyvRESNRSSLRLLGSVGEPINPEAWNwyyNVVGEGRCP-IVDTWWQTETggiliAPLPGATA------LKPGSATR 437
Cdd:PRK12583 308 HPQR--GNFDLSSLRTGIMAGAPCPIEVMR---RVMDEMHMAeVQIAYGMTET-----SPVSLQTTaaddleRRVETVGR 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 438 PLFGIQPAIVDGEGNELEGAAEGNLVIKDSwpGQMRTIWGDPDRFIEAYFStfKNTYFTGDGARRDEDGYYWITGRVDDV 517
Cdd:PRK12583 378 TQPHLEVKVVDPDGATVPRGEIGELCTRGY--SVMKGYWNNPEATAESIDE--DGWMHTGDLATMDEQGYVRIVGRSKDM 453
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 518 LNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEElrkELISWVRKVLGPVASPDALHWA 597
Cdd:PRK12583 454 IIRGGENIYPREIEEFLFTHPAVADVQVFGVPDEKYGEEIVAWVRLHPGHAASEE---ELREFCKARIAHFKVPRYFRFV 530
|
570 580
....*....|....*....|....
gi 446925704 598 PALPKTRSGKIMRRILRKIAANEL 621
Cdd:PRK12583 531 DEFPMTVTGKVQKFRMREISIEEL 554
|
|
| PRK06145 |
PRK06145 |
acyl-CoA synthetase; Validated |
83-614 |
2.44e-34 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 102207 [Multi-domain] Cd Length: 497 Bit Score: 136.94 E-value: 2.44e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 83 HLKEHPHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFG 162
Cdd:PRK06145 11 HARRTPDRAALVYRDQE------ISYAEFHQRILQAAGMLHARGIGQGDVVALLMKNSAAFLELAFAASYLGAVFLPINY 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 163 GFSPDSLASRIEDSQAKLvitadsslragklLPLKENVDlalALPGTECVENVIVVYRNANPIEMKPGrdlwyhliimev 242
Cdd:PRK06145 85 RLAADEVAYILGDAGAKL-------------LLVDEEFD---AIVALETPKIVIDAAAQADSRRLAQG------------ 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 243 DANCPPEPMKAEDPLF-ILYTSGSTGKPKGVLHTTGgylvyvastfkevfdlkqdDVYWCTAD----VGWITGHSYLIYG 317
Cdd:PRK06145 137 GLEIPPQAAVAPTDLVrLMYTSGTTDRPKGVMHSYG-------------------NLHWKSIDhviaLGLTASERLLVVG 197
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 318 PLAN---------------GTTTLMFEGVPQYPTWArlghvVDKHKVSILYTAPTAIRAMMREGDSYvrESNRSSLRLLG 382
Cdd:PRK06145 198 PLYHvgafdlpgiavlwvgGTLRIHREFDPEAVLAA-----IERHRLTCAWMAPVMLSRVLTVPDRD--RFDLDSLAWCI 270
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 383 SVGEPINPEAWNWYYNVVGEGRcpIVDTWWQTETGGILIAPLPGATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNL 462
Cdd:PRK06145 271 GGGEKTPESRIRDFTRVFTRAR--YIDAYGLTETCSGDTLMEAGREIEKIGSTGRALAHVEIRIADGAGRWLPPNMKGEI 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 463 VIKDswPGQMRTIWGDPDRFIEAYFSTFkntYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAE 542
Cdd:PRK06145 349 CMRG--PKVTKGYWKDPEKTAEAFYGDW---FRSGDVGYLDEEGFLYLTDRKKDMIISGGENIASSEVERVIYELPEVAE 423
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 446925704 543 AAVVGMPHDIKGQGICTFVTLQAGVP-ESEELRKEliswVRKVLGPVASPDALHWAPALPKTRSGKIMRRILR 614
Cdd:PRK06145 424 AAVIGVHDDRWGERITAVVVLNPGATlTLEALDRH----CRQRLASFKVPRQLKVRDELPRNPSGKVLKRVLR 492
|
|
| FAA1 |
COG1022 |
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; |
80-547 |
4.68e-34 |
|
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
Pssm-ID: 440645 [Multi-domain] Cd Length: 603 Bit Score: 137.54 E-value: 4.68e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIWEGDHPSRHkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCV 159
Cdd:COG1022 17 LRRRAARFPDRVALREKEDGIWQS--LTWAEFAERVRALAAGLLALGVKPGDRVAILSDNRPEWVIADLAILAAGAVTVP 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFGGFSPDSLASRIEDSQAKLVITADSSLRAgKLLPLKENVdlalalpgtECVENVIVVyrnaNPIEMKPGRDL--WYHL 237
Cdd:COG1022 95 IYPTSSAEEVAYILNDSGAKVLFVEDQEQLD-KLLEVRDEL---------PSLRHIVVL----DPRGLRDDPRLlsLDEL 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 238 IIMEVDANCPPE------PMKAEDPLFILYTSGSTGKPKGVLHTTGGyLVYVASTFKEVFDLKQDDVY-----WCtadvg 306
Cdd:COG1022 161 LALGREVADPAElearraAVKPDDLATIIYTSGTTGRPKGVMLTHRN-LLSNARALLERLPLGPGDRTlsflpLA----- 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 307 WITGHSYLIYGpLANGTT-------------------TLMFeGVP----------------QYPT------WA-RLG--- 341
Cdd:COG1022 235 HVFERTVSYYA-LAAGATvafaespdtlaedlrevkpTFML-AVPrvwekvyagiqakaeeAGGLkrklfrWAlAVGrry 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 342 --HVVDKHKVSILYTAPTA---------IRAMMreGDsyvresnrsSLRLLGSVGEPINPEAWNWYYNV-VgegrcPIVD 409
Cdd:COG1022 313 arARLAGKSPSLLLRLKHAladklvfskLREAL--GG---------RLRFAVSGGAALGPELARFFRALgI-----PVLE 376
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 410 TWWQTETGGILIAPLPGATalKPGSATRPLFGIQPAIvdgegnelegAAEGNLVIKDswPGQMRTIWGDPdrfiEAYFST 489
Cdd:COG1022 377 GYGLTETSPVITVNRPGDN--RIGTVGPPLPGVEVKI----------AEDGEILVRG--PNVMKGYYKNP----EATAEA 438
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446925704 490 F-KNTYF-TGDGARRDEDGYYWITGRVDDVLNVSGhrlGT----AEIESALVSHEAVAEAAVVG 547
Cdd:COG1022 439 FdADGWLhTGDIGELDEDGFLRITGRKKDLIVTSG---GKnvapQPIENALKASPLIEQAVVVG 499
|
|
| PRK06087 |
PRK06087 |
medium-chain fatty-acid--CoA ligase; |
85-615 |
7.84e-34 |
|
medium-chain fatty-acid--CoA ligase;
Pssm-ID: 180393 [Multi-domain] Cd Length: 547 Bit Score: 136.03 E-value: 7.84e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 85 KEHPHKPAIIweGDHPSRHkivSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGF 164
Cdd:PRK06087 34 RAMPDKIAVV--DNHGASY---TYSALDHAASRLANWLLAKGIEPGDRVAFQLPGWCEFTIIYLACLKVGAVSVPLLPSW 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 165 SPDSLASRIEDSQAKLVI--TADSSLRAGKL-LPLKENVDlalalpgteCVENVIVVYRNAnpiemKPGRDLWYHLIIME 241
Cdd:PRK06087 109 REAELVWVLNKCQAKMFFapTLFKQTRPVDLiLPLQNQLP---------QLQQIVGVDKLA-----PATSSLSLSQIIAD 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 242 VDANCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGyLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLAN 321
Cdd:PRK06087 175 YEPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNN-ILASERAYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLI 253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 322 GTTTLMFEgvpqyptwarlghvvdkhkvsiLYTAPTAIRAMMREGDSYV----------------RESNRSSLRLLGSVG 385
Cdd:PRK06087 254 GARSVLLD----------------------IFTPDACLALLEQQRCTCMlgatpfiydllnllekQPADLSALRFFLCGG 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 386 EPInPEAwnwyynvvgegrcpIVDTWWQTetgGILIAPLPGATALKP--------------GSATRPLFGIQPAIVDGEG 451
Cdd:PRK06087 312 TTI-PKK--------------VARECQQR---GIKLLSVYGSTESSPhavvnlddplsrfmHTDGYAAAGVEIKVVDEAR 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 452 NELEGAAEGNLVIKDswPGQMRTIWGDPDRFIEAYFStfKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIE 531
Cdd:PRK06087 374 KTLPPGCEGEEASRG--PNVFMGYLDEPELTARALDE--EGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE 449
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 532 SALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAgvPESEELRKELISWV-RKVLGPVASPDALHWAPALPKTRSGKIMR 610
Cdd:PRK06087 450 DILLQHPKIHDACVVAMPDERLGERSCAYVVLKA--PHHSLTLEEVVAFFsRKRVAKYKYPEHIVVIDKLPRTASGKIQK 527
|
....*
gi 446925704 611 RILRK 615
Cdd:PRK06087 528 FLLRK 532
|
|
| PRK06839 |
PRK06839 |
o-succinylbenzoate--CoA ligase; |
87-616 |
1.19e-33 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 168698 [Multi-domain] Cd Length: 496 Bit Score: 134.99 E-value: 1.19e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 87 HPHKPAIIWEgdhpsrHKIVSYKELHDEVCRFANVLK-KYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFS 165
Cdd:PRK06839 15 HPDRIAIITE------EEEMTYKQLHEYVSKVAAYLIyELNVKKGERIAILSQNSLEYIVLLFAIAKVECIAVPLNIRLT 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 166 PDSLASRIEDSQAKlvitadsslragKLLPLKENVDLALALPGTECVENVIVVYRNANPIEMKPgrdlwyhliimeVDAn 245
Cdd:PRK06839 89 ENELIFQLKDSGTT------------VLFVEKTFQNMALSMQKVSYVQRVISITSLKEIEDRKI------------DNF- 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 246 cppEPMKAEDPLFILYTSGSTGKPKG-VLHTTGGYLVYVASTFkeVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTT 324
Cdd:PRK06839 144 ---VEKNESASFIICYTSGTTGKPKGaVLTQENMFWNALNNTF--AIDLTMHDRSIVLLPLFHIGGIGLFAFPTLFAGGV 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 325 TLM---FEgvpqyPTWARlgHVVDKHKVSILYTAPTAIRAMMREGDsyVRESNRSSLRLLGSVGEPINPEAWNWYYN--- 398
Cdd:PRK06839 219 IIVprkFE-----PTKAL--SMIEKHKVTVVMGVPTIHQALINCSK--FETTNLQSVRWFYNGGAPCPEELMREFIDrgf 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 399 VVGEGrcpivdtWWQTETGGILIAPLPGATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDswPGQMRTIWGD 478
Cdd:PRK06839 290 LFGQG-------FGMTETSPTVFMLSEEDARRKVGSIGKPVLFCDYELIDENKNKVEVGEVGELLIRG--PNVMKEYWNR 360
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 479 PDRFIEayfsTFKNTYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGI 557
Cdd:PRK06839 361 PDATEE----TIQDGWLcTGDLARVDEDGFVYIVGRKKEMIISGGENIYPLEVEQVINKLSDVYEVAVVGRQHVKWGEIP 436
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 446925704 558 CTFVTLQAGVPESEElrkELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKI 616
Cdd:PRK06839 437 IAFIVKKSSSVLIEK---DVIEHCRLFLAKYKIPKEIVFLKELPKNATGKIQKAQLVNQ 492
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
85-621 |
8.84e-33 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 135.86 E-value: 8.84e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 85 KEHPHKPAIIWEGdhpsrhKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGF 164
Cdd:PRK12316 4562 RMTPDAVAVVFDE------EKLTYAELNRRANRLAHALIARGVGPEVLVGIAMERSAEMMVGLLAVLKAGGAYVPLDPEY 4635
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 165 SPDSLASRIEDSQAKLVITaDSSLRAGklLPLKENVDlALALPGTECVEnvivvyrnanpiemkpGRdlwyhliimevDA 244
Cdd:PRK12316 4636 PRERLAYMMEDSGAALLLT-QSHLLQR--LPIPDGLA-SLALDRDEDWE----------------GF-----------PA 4684
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 245 NCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVASTfKEVFDLKQDDVYWCTADVGWITGHSYLiYGPLANGTT 324
Cdd:PRK12316 4685 HDPAVRLHPDNLAYVIYTSGSTGRPKGVAVSHGSLVNHLHAT-GERYELTPDDRVLQFMSFSFDGSHEGL-YHPLINGAS 4762
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 325 TLMFEgvPQYPTWARLGHVVDKHKVSILYTAPTAIRaMMREGDSyvRESNRSSLRLLGSVGEPINP----EAWN-----W 395
Cdd:PRK12316 4763 VVIRD--DSLWDPERLYAEIHEHRVTVLVFPPVYLQ-QLAEHAE--RDGEPPSLRVYCFGGEAVAQasydLAWRalkpvY 4837
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 396 YYNVVGEGRCPIVDTWWQTETGgiliaPLPGATALKPGsatRPLFGIQPAIVDGEGNELEGAAEGNLVIKDSwpGQMRTI 475
Cdd:PRK12316 4838 LFNGYGPTETTVTVLLWKARDG-----DACGAAYMPIG---TPLGNRSGYVLDGQLNPLPVGVAGELYLGGE--GVARGY 4907
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 476 WGDPD----RFIEAYFSTF-KNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPH 550
Cdd:PRK12316 4908 LERPAltaeRFVPDPFGAPgGRLYRTGDLARYRADGVIDYLGRVDHQVKIRGFRIELGEIEARLREHPAVREAVVIAQEG 4987
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446925704 551 DIKGQGICTFVTLQAGVPESEELRKELISWVRKVLGPVAsPD---ALHWA--PALPKTRSGKIMRRILRKIAANEL 621
Cdd:PRK12316 4988 AVGKQLVGYVVPQDPALADADEAQAELRDELKAALRERL-PEymvPAHLVflARMPLTPNGKLDRKALPQPDASLL 5062
|
|
| PRK06155 |
PRK06155 |
crotonobetaine/carnitine-CoA ligase; Provisional |
80-616 |
1.05e-32 |
|
crotonobetaine/carnitine-CoA ligase; Provisional
Pssm-ID: 235719 [Multi-domain] Cd Length: 542 Bit Score: 132.96 E-value: 1.05e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCV 159
Cdd:PRK06155 27 LARQAERYPDRPLLVFGGTR------WTYAEAARAAAAAAHALAAAGVKRGDRVALMCGNRIEFLDVFLGCAWLGAIAVP 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFGGFSPDSLASRIEDSQAKLVItADSSLragklLPLKENVD-LALALPGTECVENVIVVYRNANP--IEMKPGrdlwyh 236
Cdd:PRK06155 101 INTALRGPQLEHILRNSGARLLV-VEAAL-----LAALEAADpGDLPLPAVWLLDAPASVSVPAGWstAPLPPL------ 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 237 liimevDANCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYL---VYVAstfkEVFDLKQDDVYWCTADVGWITGHSY 313
Cdd:PRK06155 169 ------DAPAPAAAVQPGDTAAILYTSGTTGPSKGVCCPHAQFYwwgRNSA----EDLEIGADDVLYTTLPLFHTNALNA 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 314 LiYGPLANGTTTLMFEGVPQYPTWARlghvVDKHK----------VSILYTAPTairammregdsyvRESNR-SSLRL-L 381
Cdd:PRK06155 239 F-FQALLAGATYVLEPRFSASGFWPA----VRRHGatvtyllgamVSILLSQPA-------------RESDRaHRVRVaL 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 382 GSVGEPINPEAWNWYYNVvgegrcPIVDTWWQTETGGILIAPLPGAtalKPGSATRPLFGIQPAIVDGEGNELEGAAEGN 461
Cdd:PRK06155 301 GPGVPAALHAAFRERFGV------DLLDGYGSTETNFVIAVTHGSQ---RPGSMGRLAPGFEARVVDEHDQELPDGEPGE 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 462 LVIKDSWPGQMRT-IWGDPDRFIEAYfstfKNTYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEA 539
Cdd:PRK06155 372 LLLRADEPFAFATgYFGMPEKTVEAW----RNLWFhTGDRVVRDADGWFRFVDRIKDAIRRRGENISSFEVEQVLLSHPA 447
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 540 VAEAAVVGMPHDIKGQGICTFVTLQAGV---PEseelrkELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKI 616
Cdd:PRK06155 448 VAAAAVFPVPSELGEDEVMAAVVLRDGTalePV------ALVRHCEPRLAYFAVPRYVEFVAALPKTENGKVQKFVLREQ 521
|
|
| CBAL |
cd05923 |
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ... |
88-613 |
1.26e-32 |
|
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.
Pssm-ID: 341247 [Multi-domain] Cd Length: 493 Bit Score: 131.86 E-value: 1.26e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 88 PHKPAIIwegdHPSRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPD 167
Cdd:cd05923 15 PDACAIA----DPARGLRLTYSELRARIEAVAARLHARGLRPGQRVAVVLPNSVEAVIALLALHRLGAVPALINPRLKAA 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 168 SLASRIEDSQAKLVITAD-------SSLRAGKLLPLKENVDLALAlpgtecvenvivvyRNANPIEMKPgrdlwyhliim 240
Cdd:cd05923 91 ELAELIERGEMTAAVIAVdaqvmdaIFQSGVRVLALSDLVGLGEP--------------ESAGPLIEDP----------- 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 241 evdancPPEPmkaEDPLFILYTSGSTGKPKGV-------------LHTTGGYLVYVASTFKEVFDLKQddvywctadvgw 307
Cdd:cd05923 146 ------PREP---EQPAFVFYTSGTTGLPKGAvipqraaesrvlfMSTQAGLRHGRHNVVLGLMPLYH------------ 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 308 ITGHSYLIYGPLANGTTTLmfegVPQYPTWARLGHVVDKHKVSILYTAPTAIRAMMreGDSYVRESNRSSLRLLGSVGEP 387
Cdd:cd05923 205 VIGFFAVLVAALALDGTYV----VVEEFDPADALKLIEQERVTSLFATPTHLDALA--AAAEFAGLKLSSLRHVTFAGAT 278
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 388 InPEAWNWYYNVVGEGrcPIVDTWWQTETGGILIAPLPgatalKPGSATRPLFGIQPAIVDGEGNELEGAA---EGNLVI 464
Cdd:cd05923 279 M-PDAVLERVNQHLPG--EKVNIYGTTEAMNSLYMRDA-----RTGTEMRPGFFSEVRIVRIGGSPDEALAngeEGELIV 350
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 465 KDSWPGQMRTIWGDPDrfieayfSTFKNT----YFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAV 540
Cdd:cd05923 351 AAAADAAFTGYLNQPE-------ATAKKLqdgwYRTGDVGYVDPSGDVRILGRVDDMIISGGENIHPSEIERVLSRHPGV 423
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446925704 541 AEAAVVGMPHDIKGQGICTFVTLQAGVPESEELRK-----ELISWVRkvlgpvasPDALHWAPALPKTRSGKIMRRIL 613
Cdd:cd05923 424 TEVVVIGVADERWGQSVTACVVPREGTLSADELDQfcrasELADFKR--------PRRYFFLDELPKNAMNKVLRRQL 493
|
|
| PRK13390 |
PRK13390 |
acyl-CoA synthetase; Provisional |
83-614 |
1.37e-32 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139538 [Multi-domain] Cd Length: 501 Bit Score: 132.05 E-value: 1.37e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 83 HLKEHPHKPAIIWegdhPSRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFG 162
Cdd:PRK13390 6 HAQIAPDRPAVIV----AETGEQVSYRQLDDDSAALARVLYDAGLRTGDVVALLSDNSPEALVVLWAALRSGLYITAINH 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 163 GFSPDSLASRIEDSQAKlVITADSSL-----RAGKLLPLKenVDLALALPGTECVENVIVvyrnanpiemkpgrdlwyhl 237
Cdd:PRK13390 82 HLTAPEADYIVGDSGAR-VLVASAALdglaaKVGADLPLR--LSFGGEIDGFGSFEAALA-------------------- 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 238 iimevdANCPPepmKAEDPL--FILYTSGSTGKPKGV--------LHTTGGYLVYVASTFkevFDLKQDDVYWCTADVGw 307
Cdd:PRK13390 139 ------GAGPR---LTEQPCgaVMLYSSGTTGFPKGIqpdlpgrdVDAPGDPIVAIARAF---YDISESDIYYSSAPIY- 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 308 itgHSyliyGPL-------ANGTTTLMfegVPQYPTWARLGHVvDKHKVSILYTAPTAIRAMMREGDSYVRESNRSSLRL 380
Cdd:PRK13390 206 ---HA----APLrwcsmvhALGGTVVL---AKRFDAQATLGHV-ERYRITVTQMVPTMFVRLLKLDADVRTRYDVSSLRA 274
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 381 LGSVGEPINPEAWNWYYNVVGegrcPIVDTWWQ-TETGGILIAPLPGATAlKPGSATRPLFGiQPAIVDGEGNELEGAAE 459
Cdd:PRK13390 275 VIHAAAPCPVDVKHAMIDWLG----PIVYEYYSsTEAHGMTFIDSPDWLA-HPGSVGRSVLG-DLHICDDDGNELPAGRI 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 460 GNLVI-KDSWPGQMRTiwgDPDRFIEAYFSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHE 538
Cdd:PRK13390 349 GTVYFeRDRLPFRYLN---DPEKTAAAQHPAHPFWTTVGDLGSVDEDGYLYLADRKSFMIISGGVNIYPQETENALTMHP 425
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446925704 539 AVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILR 614
Cdd:PRK13390 426 AVHDVAVIGVPDPEMGEQVKAVIQLVEGIRGSDELARELIDYTRSRIAHYKAPRSVEFVDELPRTPTGKLVKGLLR 501
|
|
| PRK07798 |
PRK07798 |
acyl-CoA synthetase; Validated |
88-607 |
5.21e-32 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236100 [Multi-domain] Cd Length: 533 Bit Score: 130.78 E-value: 5.21e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 88 PHKPAIIWeGDHpsrhkIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPD 167
Cdd:PRK07798 17 PDRVALVC-GDR-----RLTYAELEERANRLAHYLIAQGLGPGDHVGIYARNRIEYVEAMLGAFKARAVPVNVNYRYVED 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 168 SLASRIEDSQAKLVItADSSLRAgkllplkenvDLALALPGTECVENVIVVYRNANPIEMKPGRDlwYHLIIMEVDANCP 247
Cdd:PRK07798 91 ELRYLLDDSDAVALV-YEREFAP----------RVAEVLPRLPKLRTLVVVEDGSGNDLLPGAVD--YEDALAAGSPERD 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 248 PEPMKAEDpLFILYTSGSTGKPKGVLHTT--------GGYLVYVASTFKEVFDLkqddvywcTADVGWITGHSYLIYGPL 319
Cdd:PRK07798 158 FGERSPDD-LYLLYTGGTTGMPKGVMWRQedifrvllGGRDFATGEPIEDEEEL--------AKRAAAGPGMRRFPAPPL 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 320 ANGTTTL-----MFEG--VPQYPTW----ARLGHVVDKHKVSILYTAPTAI-RAMMREGDSyVRESNRSSLRLLGSVGEP 387
Cdd:PRK07798 229 MHGAGQWaafaaLFSGqtVVLLPDVrfdaDEVWRTIEREKVNVITIVGDAMaRPLLDALEA-RGPYDLSSLFAIASGGAL 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 388 INP---EAW-NWYYNVVgegrcpIVDTWWQTETGgiliAPLPGATALKPGSATRPLFGIQP--AIVDGEGNELEGAAEGn 461
Cdd:PRK07798 308 FSPsvkEALlELLPNVV------LTDSIGSSETG----FGGSGTVAKGAVHTGGPRFTIGPrtVVLDEDGNPVEPGSGE- 376
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 462 lvikDSWPGQMRTI----WGDPdrfiEAYFSTFKN----TY-FTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIES 532
Cdd:PRK07798 377 ----IGWIARRGHIplgyYKDP----EKTAETFPTidgvRYaIPGDRARVEADGTITLLGRGSVCINTGGEKVFPEEVEE 448
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 446925704 533 ALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEElrkELISWVRKVLGPVASPDALHWAPALPKTRSGK 607
Cdd:PRK07798 449 ALKAHPDVADALVVGVPDERWGQEVVAVVQLREGARPDLA---ELRAHCRSSLAGYKVPRAIWFVDEVQRSPAGK 520
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
107-613 |
1.31e-31 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 132.39 E-value: 1.31e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 107 SYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITaDS 186
Cdd:PRK12316 538 DYAELNRRANRLAHALIERGVGPDVLVGVAMERSIEMVVALLAILKAGGAYVPLDPEYPAERLAYMLEDSGVQLLLS-QS 616
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 187 SLraGKLLPLKENVD-LALALPGTEcVENvivvYRNANP-IEMKPgrdlwyhliimevdancppepmkaEDPLFILYTSG 264
Cdd:PRK12316 617 HL--GRKLPLAAGVQvLDLDRPAAW-LEG----YSEENPgTELNP------------------------ENLAYVIYTSG 665
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 265 STGKPKGVLHTTGGyLVYVASTFKEVFDLKQDDVYWCTADVGWITGHsYLIYGPLANGTT-TLMFEGVPQYPtwARLGHV 343
Cdd:PRK12316 666 STGKPKGAGNRHRA-LSNRLCWMQQAYGLGVGDTVLQKTPFSFDVSV-WEFFWPLMSGARlVVAAPGDHRDP--AKLVEL 741
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 344 VDKHKVSILYTAPTAIRAMMREGDsyvrESNRSSLRLLGSVGEPINPEA--------WNW-YYNVVGEGRCPIVDTWWqt 414
Cdd:PRK12316 742 INREGVDTLHFVPSMLQAFLQDED----VASCTSLRRIVCSGEALPADAqeqvfaklPQAgLYNLYGPTEAAIDVTHW-- 815
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 415 etggiliaplpgaTALKPGSAT----RPLFGIQPAIVDGEGNELEGAAEGNLVIKDSwpGQMRTIWGDP----DRFIEAY 486
Cdd:PRK12316 816 -------------TCVEEGGDSvpigRPIANLACYILDANLEPVPVGVLGELYLAGR--GLARGYHGRPgltaERFVPSP 880
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 487 FSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGmphdIKGQGICTFVTLQAg 566
Cdd:PRK12316 881 FVAGERMYRTGDLARYRADGVIEYAGRIDHQVKLRGLRIELGEIEARLLEHPWVREAAVLA----VDGKQLVGYVVLES- 955
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 446925704 567 vpESEELRKELISWVRKVLgpvasPDAL---HWAP--ALPKTRSGKIMRRIL 613
Cdd:PRK12316 956 --EGGDWREALKAHLAASL-----PEYMvpaQWLAleRLPLTPNGKLDRKAL 1000
|
|
| FACL_like_4 |
cd05944 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
261-618 |
2.28e-31 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341266 [Multi-domain] Cd Length: 359 Bit Score: 125.67 E-value: 2.28e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 261 YTSGSTGKPKGVLHTTGGyLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTtLMFEGVPQYPTWARL 340
Cdd:cd05944 9 HTGGTTGTPKLAQHTHSN-EVYNAWMLALNSLFDPDDVLLCGLPLFHVNGSVVTLLTPLASGAH-VVLAGPAGYRNPGLF 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 341 GH---VVDKHKVSILYTAPTAIRAMM-REGDSYVresnrSSLRLLGSVGEPINPEAWNWYYNVVGegrCPIVDTWWQTET 416
Cdd:cd05944 87 DNfwkLVERYRITSLSTVPTVYAALLqVPVNADI-----SSLRFAMSGAAPLPVELRARFEDATG---LPVVEGYGLTEA 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 417 GgILIAPLPGATALKPGSATRPL--FGIQPAIVDGEGNELEGAAE---GNLVIK--DSWPGQMRTIwGDPDRFIEAYFst 489
Cdd:cd05944 159 T-CLVAVNPPDGPKRPGSVGLRLpyARVRIKVLDGVGRLLRDCAPdevGEICVAgpGVFGGYLYTE-GNKNAFVADGW-- 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 490 fkntYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPE 569
Cdd:cd05944 235 ----LNTGDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDAHAGELPVAYVQLKPGAVV 310
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|
gi 446925704 570 SEElrkELISWVRK-VLGPVASPDALHWAPALPKTRSGKIMRRILRKIAA 618
Cdd:cd05944 311 EEE---ELLAWARDhVPERAAVPKHIEVLEELPVTAVGKVFKPALRADAI 357
|
|
| VL_LC_FACS_like |
cd05907 |
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ... |
106-547 |
2.52e-31 |
|
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341233 [Multi-domain] Cd Length: 452 Bit Score: 127.33 E-value: 2.52e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 106 VSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITAD 185
Cdd:cd05907 6 ITWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEAKALFVED 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 186 sslragkllplkenvdlalalpgtecvenvivvyrnanpiemkpgrdlwyhliimevdancppepmkAEDPLFILYTSGS 265
Cdd:cd05907 86 -------------------------------------------------------------------PDDLATIIYTSGT 98
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 266 TGKPKGVLHTTGGyLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTT-------------------TL 326
Cdd:cd05907 99 TGRPKGVMLSHRN-ILSNALALAERLPATEGDRHLSFLPLAHVFERRAGLYVPLLAGARiyfassaetllddlsevrpTV 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 327 MFeGVPQyptwarlghVVDKHKVSILYTAPTAIRAMMregdsyVRESNRSSLRLLGSVGEPINPEAWNWyYNVVGegrCP 406
Cdd:cd05907 178 FL-AVPR---------VWEKVYAAIKVKAVPGLKRKL------FDLAVGGRLRFAASGGAPLPAELLHF-FRALG---IP 237
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 407 IVDTWWQTETGGILIAPLPGatALKPGSATRPLFGIQPAIVDgegnelegaaEGNLVIKDswPGQMRTIWGDPDRFIEAY 486
Cdd:cd05907 238 VYEGYGLTETSAVVTLNPPG--DNRIGTVGKPLPGVEVRIAD----------DGEILVRG--PNVMLGYYKNPEATAEAL 303
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 446925704 487 FStfKNTYFTGDGARRDEDGYYWITGRVDDVL-NVSGHRLGTAEIESALVSHEAVAEAAVVG 547
Cdd:cd05907 304 DA--DGWLHTGDLGEIDEDGFLHITGRKKDLIiTSGGKNISPEPIENALKASPLISQAVVIG 363
|
|
| ttLC_FACS_AEE21_like |
cd12118 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ... |
80-615 |
9.52e-31 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.
Pssm-ID: 341283 [Multi-domain] Cd Length: 486 Bit Score: 126.26 E-value: 9.52e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIwegdHPSRHkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCV 159
Cdd:cd12118 10 LERAAAVYPDRTSIV----YGDRR--YTWRQTYDRCRRLASALAALGISRGDTVAVLAPNTPAMYELHFGVPMAGAVLNA 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFGGFSPDSLASRIEDSQAKLVItadsslragkllplkenVDlalalpgtecvenvivvyrnanpiemkpgRDLWYHLII 239
Cdd:cd12118 84 LNTRLDAEEIAFILRHSEAKVLF-----------------VD-----------------------------REFEYEDLL 117
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 240 MEVDANCPPEPMKAE-DPLFILYTSGSTGKPKGVLHT-TGGYLVYVASTFKevFDLKQDDVYWCTADV----GWItghsy 313
Cdd:cd12118 118 AEGDPDFEWIPPADEwDPIALNYTSGTTGRPKGVVYHhRGAYLNALANILE--WEMKQHPVYLWTLPMfhcnGWC----- 190
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 314 LIYGPLANGTTTLMFEGVpQYPTWARLghvVDKHKVSILYTAPTaIRAMMREGdsyvRESNRSSL--RLLGSVGEPINPE 391
Cdd:cd12118 191 FPWTVAAVGGTNVCLRKV-DAKAIYDL---IEKHKVTHFCGAPT-VLNMLANA----PPSDARPLphRVHVMTAGAPPPA 261
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 392 AwnwYYNVVGEGRCPIVDTWWQTET-GGILIAP-------LPGAT--ALKP--GSATRPLFGI--------QPAIVDGE- 450
Cdd:cd12118 262 A---VLAKMEELGFDVTHVYGLTETyGPATVCAwkpewdeLPTEEraRLKArqGVRYVGLEEVdvldpetmKPVPRDGKt 338
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 451 -GnelEGAAEGNLVikdswpgqMRTIWGDPdrfiEAYFSTFKNTYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTA 528
Cdd:cd12118 339 iG---EIVFRGNIV--------MKGYLKNP----EATAEAFRGGWFhSGDLAVIHPDGYIEIKDRSKDIIISGGENISSV 403
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 529 EIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEElrkELISWVRKVLGPVASPDALHWAPaLPKTRSGKI 608
Cdd:cd12118 404 EVEGVLYKHPAVLEAAVVARPDEKWGEVPCAFVELKEGAKVTEE---EIIAFCREHLAGFMVPKTVVFGE-LPKTSTGKI 479
|
....*..
gi 446925704 609 MRRILRK 615
Cdd:cd12118 480 QKFVLRD 486
|
|
| PRK06060 |
PRK06060 |
p-hydroxybenzoic acid--AMP ligase FadD22; |
98-614 |
1.95e-30 |
|
p-hydroxybenzoic acid--AMP ligase FadD22;
Pssm-ID: 180374 [Multi-domain] Cd Length: 705 Bit Score: 127.07 E-value: 1.95e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 98 DHPSRH--KIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIED 175
Cdd:PRK06060 21 DRPAFYaaDVVTHGQIHDGAARLGEVLRNRGLSSGDRVLLCLPDSPDLVQLLLACLARGVMAFLANPELHRDDHALAARN 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 176 SQAKLVITADsslragkllPLKENVDLALALPGTECVENVIvvyrNANPIEMkpgrdlwyhliimevdancppEPMKAED 255
Cdd:PRK06060 101 TEPALVVTSD---------ALRDRFQPSRVAEAAELMSEAA----RVAPGGY---------------------EPMGGDA 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 256 PLFILYTSGSTGKPKGVLHTTGGYLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLmfegVPQYP 335
Cdd:PRK06060 147 LAYATYTSGTTGPPKAAIHRHADPLTFVDAMCRKALRLTPEDTGLCSARMYFAYGLGNSVWFPLATGGSAV----INSAP 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 336 TWARLGHVVD-KHKVSILYTAPTairAMMREGDSYVRESNRsSLRLLGSVGEPINP---EAWNWYYnvvgeGRCPIVDTW 411
Cdd:PRK06060 223 VTPEAAAILSaRFGPSVLYGVPN---FFARVIDSCSPDSFR-SLRCVVSAGEALELglaERLMEFF-----GGIPILDGI 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 412 WQTETGGILIAplPGATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDswPGQMRTIWGDPDRFIEAyfstfK 491
Cdd:PRK06060 294 GSTEVGQTFVS--NRVDEWRLGTLGRVLPPYEIRVVAPDGTTAGPGVEGDLWVRG--PAIAKGYWNRPDSPVAN-----E 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 492 NTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESE 571
Cdd:PRK06060 365 GWLDTRDRVCIDSDGWVTYRCRADDTEVIGGVNVDPREVERLIIEDEAVAEAAVVAVRESTGASTLQAFLVATSGATIDG 444
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 446925704 572 ELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILR 614
Cdd:PRK06060 445 SVMRDLHRGLLNRLSAFKVPHRFAVVDRLPRTPNGKLVRGALR 487
|
|
| A_NRPS_CmdD_like |
cd17652 |
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ... |
88-613 |
2.15e-30 |
|
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).
Pssm-ID: 341307 [Multi-domain] Cd Length: 436 Bit Score: 124.29 E-value: 2.15e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 88 PHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPD 167
Cdd:cd17652 1 PDAPAVVFGDET------LTYAELNARANRLARLLAARGVGPERLVALALPRSAELVVAILAVLKAGAAYLPLDPAYPAE 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 168 SLASRIEDSQAKLVITadsslragkllplkenvdlalalpgtecvenvivvyrnanpiemkpgrdlwyhliimevdancp 247
Cdd:cd17652 75 RIAYMLADARPALLLT---------------------------------------------------------------- 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 248 pepmKAEDPLFILYTSGSTGKPKGVLHTTGGyLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLm 327
Cdd:cd17652 91 ----TPDNLAYVIYTSGSTGRPKGVVVTHRG-LANLAAAQIAAFDVGPGSRVLQFASPSFDASVWELLMALLAGATLVL- 164
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 328 fegVPQYPTWA--RLGHVVDKHKVSILYTAPTAIRAMmrEGDSYvresnrSSLRLLGSVGEPINPE-AWNW-----YYNV 399
Cdd:cd17652 165 ---APAEELLPgePLADLLREHRITHVTLPPAALAAL--PPDDL------PDLRTLVVAGEACPAElVDRWapgrrMINA 233
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 400 VGEGRCPIVDTWWQTETGGILIA---PLPGATALKPGSATRPLfgiqPAIVDGEgnelegaaegnLVIkdSWPGQMRTIW 476
Cdd:cd17652 234 YGPTETTVCATMAGPLPGGGVPPigrPVPGTRVYVLDARLRPV----PPGVPGE-----------LYI--AGAGLARGYL 296
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 477 GDPD----RFIEAYFSTF-KNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHD 551
Cdd:cd17652 297 NRPGltaeRFVADPFGAPgSRMYRTGDLARWRADGQLEFLGRADDQVKIRGFRIELGEVEAALTEHPGVAEAVVVVRDDR 376
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 446925704 552 IKGQGICTFVTLQAGV-PESEELRkeliSWVRKVLGPVASPDALHWAPALPKTRSGKIMRRIL 613
Cdd:cd17652 377 PGDKRLVAYVVPAPGAaPTAAELR----AHLAERLPGYMVPAAFVVLDALPLTPNGKLDRRAL 435
|
|
| PRK12406 |
PRK12406 |
long-chain-fatty-acid--CoA ligase; Provisional |
101-614 |
3.30e-30 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 183506 [Multi-domain] Cd Length: 509 Bit Score: 124.81 E-value: 3.30e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 101 SRHKivSYKELHDEVCRFANVLKKYGIGKGDRVVLYMP---MVTEAAiamLACARIGAVHCVVFGGFSPDSLASRIEDSQ 177
Cdd:PRK12406 9 DRRR--SFDELAQRAARAAGGLAALGVRPGDCVALLMRndfAFFEAA---YAAMRLGAYAVPVNWHFKPEEIAYILEDSG 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 178 AK-LVITADSSLRAGKLLPlkENVDLALALPGTECVENVIVVYRNANPIEMKPGRDLWyhliimeVDANCPPEPMKAEDP 256
Cdd:PRK12406 84 ARvLIAHADLLHGLASALP--AGVTVLSVPTPPEIAAAYRISPALLTPPAGAIDWEGW-------LAQQEPYDGPPVPQP 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 257 LFILYTSGSTGKPKGVLHT--TGGYLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMfegvPQY 334
Cdd:PRK12406 155 QSMIYTSGTTGHPKGVRRAapTPEQAAAAEQMRALIYGLKPGIRALLTGPLYHSAPNAYGLRAGRLGGVLVLQ----PRF 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 335 PTWARLgHVVDKHKVSILYTAPTAIRAMMREGDSYVRESNRSSLRLLGSVGEPINPEA----WNWYYNVVGEgrcpivdT 410
Cdd:PRK12406 231 DPEELL-QLIERHRITHMHMVPTMFIRLLKLPEEVRAKYDVSSLRHVIHAAAPCPADVkramIEWWGPVIYE-------Y 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 411 WWQTETGGILIAPLPGATAlKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVikdswpgqMR-------TIWGDPDRFI 483
Cdd:PRK12406 303 YGSTESGAVTFATSEDALS-HPGTVGKAAPGAELRFVDEDGRPLPQGEIGEIY--------SRiagnpdfTYHNKPEKRA 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 484 EAYFSTFKNTyftGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTL 563
Cdd:PRK12406 374 EIDRGGFITS---GDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGIPDAEFGEALMAVVEP 450
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 446925704 564 QAGV-PESEELRKELiswvRKVLGPVASPDALHWAPALPKTRSGKIMRRILR 614
Cdd:PRK12406 451 QPGAtLDEADIRAQL----KARLAGYKVPKHIEIMAELPREDSGKIFKRRLR 498
|
|
| PLN02246 |
PLN02246 |
4-coumarate--CoA ligase |
79-619 |
4.61e-30 |
|
4-coumarate--CoA ligase
Pssm-ID: 215137 [Multi-domain] Cd Length: 537 Bit Score: 124.71 E-value: 4.61e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 79 CLDRhLKEHPHKPAIIwegDHPSRHkIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHC 158
Cdd:PLN02246 29 CFER-LSEFSDRPCLI---DGATGR-VYTYADVELLSRRVAAGLHKLGIRQGDVVMLLLPNCPEFVLAFLGASRRGAVTT 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 159 VVFGGFSPDSLASRIEDSQAKLVITadSSLRAGKLLPLKENvdlalalpgtecvENVIVVYRNANPIEMKPGRDLwyhli 238
Cdd:PLN02246 104 TANPFYTPAEIAKQAKASGAKLIIT--QSCYVDKLKGLAED-------------DGVTVVTIDDPPEGCLHFSEL----- 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 239 IMEVDANCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVAstfKEV------FDLKQDDVYWCTADVGWITG-H 311
Cdd:PLN02246 164 TQADENELPEVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVA---QQVdgenpnLYFHSDDVILCVLPMFHIYSlN 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 312 SYLIYGPLANGTTTLMfegvPQYPTWARLGhVVDKHKVSILYTAPTAIRAMMRegDSYVRESNRSSLRLLGSVGEPIN-- 389
Cdd:PLN02246 241 SVLLCGLRVGAAILIM----PKFEIGALLE-LIQRHKVTIAPFVPPIVLAIAK--SPVVEKYDLSSIRMVLSGAAPLGke 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 390 ---------PEAwnwyynVVGEGrcpivdtWWQTETGGILIAPLPGA---TALKPGSATRPLFGIQPAIVDGE-GNELEG 456
Cdd:PLN02246 314 ledafraklPNA------VLGQG-------YGMTEAGPVLAMCLAFAkepFPVKSGSCGTVVRNAELKIVDPEtGASLPR 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 457 AAEGNLVIKDswPGQMRTIWGDPdrfiEAYFSTFKNTYF--TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESAL 534
Cdd:PLN02246 381 NQPGEICIRG--PQIMKGYLNDP----EATANTIDKDGWlhTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALL 454
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 535 VSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEELRKELIS-------WVRKVlgpvaspdalHWAPALPKTRSGK 607
Cdd:PLN02246 455 ISHPSIADAAVVPMKDEVAGEVPVAFVVRSNGSEITEDEIKQFVAkqvvfykRIHKV----------FFVDSIPKAPSGK 524
|
570
....*....|...
gi 446925704 608 IMRRILR-KIAAN 619
Cdd:PLN02246 525 ILRKDLRaKLAAG 537
|
|
| A_NRPS_TlmIV_like |
cd12114 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ... |
88-613 |
8.74e-30 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
Pssm-ID: 341279 [Multi-domain] Cd Length: 477 Bit Score: 123.15 E-value: 8.74e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 88 PHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVfggfSPD 167
Cdd:cd12114 1 PDATAVICGDGT------LTYGELAERARRVAGALKAAGVRPGDLVAVTLPKGPEQVVAVLGILAAGAAYVPV----DID 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 168 SLASRIE----DSQAKLVITADsslragkllplkenvDLALALPGTECVENVIVVYRNANPIEmkpgrdlwyhliimevd 243
Cdd:cd12114 71 QPAARREailaDAGARLVLTDG---------------PDAQLDVAVFDVLILDLDALAAPAPP----------------- 118
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 244 ancPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVAsTFKEVFDLKQDDVYWCtadvgwITGHS-----YLIYGP 318
Cdd:cd12114 119 ---PPVDVAPDDLAYVIFTSGSTGTPKGVMISHRAALNTIL-DINRRFAVGPDDRVLA------LSSLSfdlsvYDIFGA 188
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 319 LANGTTtLMFEGVPQYPTWARLGHVVDKHKVSILYTAPtAIRAMMREgdsYVRESNR--SSLRLLGSVGEPINPEawnwy 396
Cdd:cd12114 189 LSAGAT-LVLPDEARRRDPAHWAELIERHGVTLWNSVP-ALLEMLLD---VLEAAQAllPSLRLVLLSGDWIPLD----- 258
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 397 ynvvgegrcpIVDTWWQTETGGILIApLPGAT----------------ALKPGSATRPLFGIQPAIVDGEGNELEGAAEG 460
Cdd:cd12114 259 ----------LPARLRALAPDARLIS-LGGATeasiwsiyhpidevppDWRSIPYGRPLANQRYRVLDPRGRDCPDWVPG 327
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 461 NLVIkdSWPGQMRTIWGDP----DRFIEAyfSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVS 536
Cdd:cd12114 328 ELWI--GGRGVALGYLGDPeltaARFVTH--PDGERLYRTGDLGRYRPDGTLEFLGRRDGQVKVRGYRIELGEIEAALQA 403
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 446925704 537 HEAVAEAAVVGMPhDIKGQGICTFVTLQAGVPE--SEELRKELIswvrKVLGPVASPDALHWAPALPKTRSGKIMRRIL 613
Cdd:cd12114 404 HPGVARAVVVVLG-DPGGKRLAAFVVPDNDGTPiaPDALRAFLA----QTLPAYMIPSRVIALEALPLTANGKVDRAAL 477
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
88-646 |
3.00e-29 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 125.07 E-value: 3.00e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 88 PHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPD 167
Cdd:PRK12316 3071 PDAVALAFGEQR------LSYAELNRRANRLAHRLIERGVGPDVLVGVAVERSLEMVVGLLAILKAGGAYVPLDPEYPEE 3144
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 168 SLASRIEDSQAKLVITaDSSLRagklLPLKENVDLALALPGTEcvenvivVYRNANPIEMKPGRDLWYhliimevdancp 247
Cdd:PRK12316 3145 RLAYMLEDSGAQLLLS-QSHLR----LPLAQGVQVLDLDRGDE-------NYAEANPAIRTMPENLAY------------ 3200
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 248 pepmkaedplfILYTSGSTGKPKGVLHTTGGyLVYVASTFKEVFDLKQDDVYWCTADVGWiTGHSYLIYGPLANGTTTLM 327
Cdd:PRK12316 3201 -----------VIYTSGSTGKPKGVGIRHSA-LSNHLCWMQQAYGLGVGDRVLQFTTFSF-DVFVEELFWPLMSGARVVL 3267
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 328 fEGVPQYPTWARLGHVVDKHKVSILYTAPTAIRAMMREgdsyVRESNRSSLRLLGSVGEPINPE------AWNWYYNVVG 401
Cdd:PRK12316 3268 -AGPEDWRDPALLVELINSEGVDVLHAYPSMLQAFLEE----EDAHRCTSLKRIVCGGEALPADlqqqvfAGLPLYNLYG 3342
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 402 EGRCPIVDTWWQTETGGILIAPLpgatalkpgsaTRPLFGIQPAIVDGEGNELEGAAEGNLVIKDSwpGQMRTIWGDPD- 480
Cdd:PRK12316 3343 PTEATITVTHWQCVEEGKDAVPI-----------GRPIANRACYILDGSLEPVPVGALGELYLGGE--GLARGYHNRPGl 3409
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 481 ---RFIEAYFSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVgmphDIKGQGI 557
Cdd:PRK12316 3410 taeRFVPDPFVPGERLYRTGDLARYRADGVIEYIGRVDHQVKIRGFRIELGEIEARLLEHPWVREAVVL----AVDGRQL 3485
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 558 CTFVTLQAgvpESEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKIAAneldSLGDTSTLAEPAVVD 637
Cdd:PRK12316 3486 VAYVVPED---EAGDLREALKAHLKASLPEYMVPAHLLFLERMPLTPNGKLDRKALPRPDA----ALLQQDYVAPVNELE 3558
|
....*....
gi 446925704 638 QLITTVYPD 646
Cdd:PRK12316 3559 RRLAAIWAD 3567
|
|
| PRK13382 |
PRK13382 |
bile acid CoA ligase; |
88-614 |
4.52e-29 |
|
bile acid CoA ligase;
Pssm-ID: 172019 [Multi-domain] Cd Length: 537 Bit Score: 121.79 E-value: 4.52e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 88 PHKPAIIWEgdhpsrHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAvHCVVFG-GFSP 166
Cdd:PRK13382 57 PDRPGLIDE------LGTLTWRELDERSDALAAALQALPIGEPRVVGIMCRNHRGFVEALLAANRIGA-DILLLNtSFAG 129
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 167 DSLASRIEDSQAKLVITADsslragKLLPLkenVDLALA-LPGTecveNVIVVYRNAnpiemkPGRDLWYHLIIMEVDAN 245
Cdd:PRK13382 130 PALAEVVTREGVDTVIYDE------EFSAT---VDRALAdCPQA----TRIVAWTDE------DHDLTVEVLIAAHAGQR 190
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 246 CPPEPMKAEdplFILYTSGSTGKPKGVLHT-TGGYlvyvaSTFKEVFD---LKQDDVYWCTADVGWITGHSYLIYGPLAN 321
Cdd:PRK13382 191 PEPTGRKGR---VILLTSGTTGTPKGARRSgPGGI-----GTLKAILDrtpWRAEEPTVIVAPMFHAWGFSQLVLAASLA 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 322 GTTTLMFEGVPQyptwARLgHVVDKHKVSILYTAPTAIRAMMREGDSYVRESNRSSLRLLGSVGEPINPEAWNWYYNVVG 401
Cdd:PRK13382 263 CTIVTRRRFDPE----ATL-DLIDRHRATGLAVVPVMFDRIMDLPAEVRNRYSGRSLRFAAASGSRMRPDVVIAFMDQFG 337
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 402 EgrcPIVDTWWQTETGGILIAPlPGATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDSWPGQMRTIWGDPDr 481
Cdd:PRK13382 338 D---VIYNNYNATEAGMIATAT-PADLRAAPDTAGRPAEGTEIRILDQDFREVPTGEVGTIFVRNDTQFDGYTSGSTKD- 412
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 482 FIEAYFSTfkntyftGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFV 561
Cdd:PRK13382 413 FHDGFMAS-------GDVGYLDENGRLFVVGRDDEMIVSGGENVYPIEVEKTLATHPDVAEAAVIGVDDEQYGQRLAAFV 485
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 446925704 562 TLQAGVPESEElrkELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILR 614
Cdd:PRK13382 486 VLKPGASATPE---TLKQHVRDNLANYKVPRDIVVLDELPRGATGKILRRELQ 535
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
88-621 |
7.43e-29 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 123.53 E-value: 7.43e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 88 PHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPD 167
Cdd:PRK12316 2017 PEAIAVVFGDQH------LSYAELDSRANRLAHRLRARGVGPEVRVAIAAERSFELVVALLAVLKAGGAYVPLDPNYPAE 2090
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 168 SLASRIEDSQAKLVITaDSSLRAgkllplkenvdlalALPGTECVENVIVvyrnanpiemkpGRDLWYhliimevdANCP 247
Cdd:PRK12316 2091 RLAYMLEDSGAALLLT-QRHLLE--------------RLPLPAGVARLPL------------DRDAEW--------ADYP 2135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 248 ---PEPMKAEDPL-FILYTSGSTGKPKGVLHTTGGYLVYVASTfKEVFDLKQDDVYWCTADVGWITGHSYLiYGPLANGT 323
Cdd:PRK12316 2136 dtaPAVQLAGENLaYVIYTSGSTGLPKGVAVSHGALVAHCQAA-GERYELSPADCELQFMSFSFDGAHEQW-FHPLLNGA 2213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 324 TTLMFEGVPQYPtwARLGHVVDKHKVSILYTAPTAIRAMMREGDsyvRESNRSSLRLLGSVGEPINPEAWNWYYNVVGEG 403
Cdd:PRK12316 2214 RVLIRDDELWDP--EQLYDEMERHGVTILDFPPVYLQQLAEHAE---RDGRPPAVRVYCFGGEAVPAASLRLAWEALRPV 2288
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 404 RcpIVDTWWQTETggiLIAPL--------PGATALKPgsATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDSwpGQMRTI 475
Cdd:PRK12316 2289 Y--LFNGYGPTEA---VVTPLlwkcrpqdPCGAAYVP--IGRALGNRRAYILDADLNLLAPGMAGELYLGGE--GLARGY 2359
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 476 WGDP----DRFIEAYFS-TFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVgmph 550
Cdd:PRK12316 2360 LNRPgltaERFVPDPFSaSGERLYRTGDLARYRADGVVEYLGRIDHQVKIRGFRIELGEIEARLQAHPAVREAVVV---- 2435
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 446925704 551 DIKGQGICTFVTLQAGVPESEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKIAANEL 621
Cdd:PRK12316 2436 AQDGASGKQLVAYVVPDDAAEDLLAELRAWLAARLPAYMVPAHWVVLERLPLNPNGKLDRKALPKPDVSQL 2506
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
80-622 |
1.23e-28 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 122.96 E-value: 1.23e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIWEGdhpsrhKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCV 159
Cdd:PRK12467 3101 IEAQVARTPEAPALVFGD------QQLSYAELNRRANRLAHRLIAIGVGPDVLVGVAVERSVEMIVALLAVLKAGGAYVP 3174
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFGGFSPDSLASRIEDSQAKLVITADSSLRAgklLPLkENVDLALALPGTecvenvivvyrnanpiemkpgrDLWYHLii 239
Cdd:PRK12467 3175 LDPEYPRERLAYMIEDSGVKLLLTQAHLLEQ---LPA-PAGDTALTLDRL----------------------DLNGYS-- 3226
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 240 mevdANCPPEPMKAEDPLFILYTSGSTGKPKGV--------LHT---------TGGYLVYVASTFKevFDLKQDDVYWct 302
Cdd:PRK12467 3227 ----ENNPSTRVMGENLAYVIYTSGSTGKPKGVgvrhgalaNHLcwiaeayelDANDRVLLFMSFS--FDGAQERFLW-- 3298
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 303 advgwitghsyliygPLANGTTTLMFEGVPQYPtwARLGHVVDKHKVSILYTAPTAIRAMMREGDsyVRESNRSSLRLLG 382
Cdd:PRK12467 3299 ---------------TLICGGCLVVRDNDLWDP--EELWQAIHAHRISIACFPPAYLQQFAEDAG--GADCASLDIYVFG 3359
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 383 svGEPINPEAWNWY---------YNVVGEGRCPIVDTWWQTETGGIliaplPGATALKPGsatRPLFGIQPAIVDGEGNE 453
Cdd:PRK12467 3360 --GEAVPPAAFEQVkrklkprglTNGYGPTEAVVTVTLWKCGGDAV-----CEAPYAPIG---RPVAGRSIYVLDGQLNP 3429
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 454 LEGAAEGNLVIKDSwpGQMRTIWGDP----DRFIEAYFSTF-KNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTA 528
Cdd:PRK12467 3430 VPVGVAGELYIGGV--GLARGYHQRPsltaERFVADPFSGSgGRLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELG 3507
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 529 EIESALVSHEAVAEAAVVGMPHDiKGQGICTFVTLQAgvpESEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKI 608
Cdd:PRK12467 3508 EIEARLLQHPSVREAVVLARDGA-GGKQLVAYVVPAD---PQGDWRETLRDHLAASLPDYMVPAQLLVLAAMPLGPNGKV 3583
|
570
....*....|....
gi 446925704 609 MRRILRKIAANELD 622
Cdd:PRK12467 3584 DRKALPDPDAKGSR 3597
|
|
| AMP-binding_C |
pfam13193 |
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ... |
529-607 |
1.68e-28 |
|
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.
Pssm-ID: 463804 [Multi-domain] Cd Length: 76 Bit Score: 108.40 E-value: 1.68e-28
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 446925704 529 EIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVpesEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGK 607
Cdd:pfam13193 1 EVESALVSHPAVAEAAVVGVPDELKGEAPVAFVVLKPGV---ELLEEELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
|
|
| PRK05605 |
PRK05605 |
long-chain-fatty-acid--CoA ligase; Validated |
80-615 |
3.38e-28 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235531 [Multi-domain] Cd Length: 573 Bit Score: 119.33 E-value: 3.38e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIWEGdhpsrhKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVhcV 159
Cdd:PRK05605 38 YDNAVARFGDRPALDFFG------ATTTYAELGKQVRRAAAGLRALGVRPGDRVAIVLPNCPQHIVAFYAVLRLGAV--V 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFGG--FSPDSLASRIEDSQAKLVITADSSlragkllplkenVDLALALPGTECVENVIVV-YRNANPIEMK-------- 228
Cdd:PRK05605 110 VEHNplYTAHELEHPFEDHGARVAIVWDKV------------APTVERLRRTTPLETIVSVnMIAAMPLLQRlalrlpip 177
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 229 -------------PGRDLWYHL----IIMEVDANCPPEPmKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVASTFKEVF 291
Cdd:PRK05605 178 alrkaraaltgpaPGTVPWETLvdaaIGGDGSDVSHPRP-TPDDVALILYTSGTTGKPKGAQLTHRNLFANAAQGKAWVP 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 292 DL-KQDDVYWCTADVGwitgHSY-----LIYGPLANGTTTLMfeGVPQYPTWARlghVVDKHKVSILYTAPTAIRAMMRE 365
Cdd:PRK05605 257 GLgDGPERVLAALPMF----HAYgltlcLTLAVSIGGELVLL--PAPDIDLILD---AMKKHPPTWLPGVPPLYEKIAEA 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 366 GdsyvRESNRSslrlLGSVgepinpeawnwYYNVVGEGRCPiVDT--WWQTETGGILI--------APL----PGATALK 431
Cdd:PRK05605 328 A----EERGVD----LSGV-----------RNAFSGAMALP-VSTveLWEKLTGGLLVegygltetSPIivgnPMSDDRR 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 432 PGSATRPLFGIQPAIVDGE--GNELEGAAEGNLVIKDswPGQMRTIWGDPdrfiEAYFSTFKNTYF-TGDGARRDEDGYY 508
Cdd:PRK05605 388 PGYVGVPFPDTEVRIVDPEdpDETMPDGEEGELLVRG--PQVFKGYWNRP----EETAKSFLDGWFrTGDVVVMEEDGFI 461
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 509 WITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEELRKElisWVRKVLGPV 588
Cdd:PRK05605 462 RIVDRIKELIITGGFNVYPAEVEEVLREHPGVEDAAVVGLPREDGSEEVVAAVVLEPGAALDPEGLRA---YCREHLTRY 538
|
570 580
....*....|....*....|....*..
gi 446925704 589 ASPDALHWAPALPKTRSGKIMRRILRK 615
Cdd:PRK05605 539 KVPRRFYHVDELPRDQLGKVRRREVRE 565
|
|
| FADD10 |
cd17635 |
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ... |
254-610 |
5.21e-27 |
|
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.
Pssm-ID: 341290 [Multi-domain] Cd Length: 340 Bit Score: 112.35 E-value: 5.21e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 254 EDPLFILYTSGSTGKPKGVLhttggylVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGpLANGTTTLMFEGV-- 331
Cdd:cd17635 1 EDPLAVIFTSGTTGEPKAVL-------LANKTFFAVPDILQKEGLNWVVGDVTYLPLPATHIGG-LWWILTCLIHGGLcv 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 332 --PQYPTWARLGHVVDKHKVSILYTAPTAIRAMMREGDSYVRESnrSSLRLLGSVGE-PINPEAWNWYYNvvgeGRCPIV 408
Cdd:cd17635 73 tgGENTTYKSLFKILTTNAVTTTCLVPTLLSKLVSELKSANATV--PSLRLIGYGGSrAIAADVRFIEAT----GLTNTA 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 409 DTWWQTETGGILIAPLpGATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDSW--PGQMRTIWGDPDRFIEAY 486
Cdd:cd17635 147 QVYGLSETGTALCLPT-DDDSIEINAVGRPYPGVDVYLAATDGIAGPSASFGTIWIKSPAnmLGYWNNPERTAEVLIDGW 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 487 FstfkntyFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQgictfvTLQAG 566
Cdd:cd17635 226 V-------NTGDLGERREDGFLFITGRSSESINCGGVKIAPDEVERIAEGVSGVQECACYEISDEEFGE------LVGLA 292
|
330 340 350 360
....*....|....*....|....*....|....*....|....*...
gi 446925704 567 VPESEELRKELIS----WVRKVLGPVASPDALHWAPALPKTRSGKIMR 610
Cdd:cd17635 293 VVASAELDENAIRalkhTIRRELEPYARPSTIVIVTDIPRTQSGKVKR 340
|
|
| AAS_C |
cd05909 |
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ... |
106-617 |
6.56e-27 |
|
C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.
Pssm-ID: 341235 [Multi-domain] Cd Length: 490 Bit Score: 114.74 E-value: 6.56e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 106 VSYKELHDEVCRFANVLKKYGIGkGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITAD 185
Cdd:cd05909 8 LTYRKLLTGAIALARKLAKMTKE-GENVGVMLPPSAGGALANFALALSGKVPVMLNYTAGLRELRACIKLAGIKTVLTSK 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 186 SSLRAGKLLPlkenvdlalalpGTECVENVIVVYrnanpIEMKPGRDLWYHLIIMEVDANCPPEPM---------KAEDP 256
Cdd:cd05909 87 QFIEKLKLHH------------LFDVEYDARIVY-----LEDLRAKISKADKCKAFLAGKFPPKWLlrifgvapvQPDDP 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 257 LFILYTSGSTGKPKGVLHTTGGYLVYVASTFKeVFDLKQDDVywctadvgwITG-----HSYliygPLANGTTTLMFEGV 331
Cdd:cd05909 150 AVILFTSGSEGLPKGVVLSHKNLLANVEQITA-IFDPNPEDV---------VFGalpffHSF----GLTGCLWLPLLSGI 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 332 PQY----PTWAR-LGHVVDKHKVSILYTAPTAIRAMMRegdsYVRESNRSSLRLLGSVGEPINP---EAWNWYYNVvgeg 403
Cdd:cd05909 216 KVVfhpnPLDYKkIPELIYDKKATILLGTPTFLRGYAR----AAHPEDFSSLRLVVAGAEKLKDtlrQEFQEKFGI---- 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 404 rcPIVDTWWQTETGGILIAPLPgATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDSwPGQMRTIWGDPDRFI 483
Cdd:cd05909 288 --RILEGYGTTECSPVISVNTP-QSPNKEGTVGRPLPGMEVKIVSVETHEEVPIGEGGLLLVRG-PNVMLGYLNEPELTS 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 484 EAYFSTFkntYFTGDGARRDEDGYYWITGRVDdvlnvsghRLgtAEIESALVSHEAV-----------AEAAVVGMPHDI 552
Cdd:cd05909 364 FAFGDGW---YDTGDIGKIDGEGFLTITGRLS--------RF--AKIAGEMVSLEAIedilseilpedNEVAVVSVPDGR 430
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446925704 553 KGQGICTFVTLQAGVPES--EELRKELISWVRKvlgpvasPDALHWAPALPKTRSGKIMRRILRKIA 617
Cdd:cd05909 431 KGEKIVLLTTTTDTDPSSlnDILKNAGISNLAK-------PSYIHQVEEIPLLGTGKPDYVTLKALA 490
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
107-621 |
1.71e-26 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 116.03 E-value: 1.71e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 107 SYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITaDS 186
Cdd:PRK12467 1601 TYGELNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAILKAGGAYVPLDPEYPRERLAYMIEDSGIELLLT-QS 1679
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 187 SLRAGklLPLKENVDLALALPGTECVENvivvYRNANPIEMKPGRDLWYhliimevdancppepmkaedplfILYTSGST 266
Cdd:PRK12467 1680 HLQAR--LPLPDGLRSLVLDQEDDWLEG----YSDSNPAVNLAPQNLAY-----------------------VIYTSGST 1730
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 267 GKPKGVLHTTGGYLVYVASTfKEVFDLKQDDVywctadvgWITGHSYL-------IYGPLANGTTTLmfegVPQYPTW-- 337
Cdd:PRK12467 1731 GRPKGAGNRHGALVNRLCAT-QEAYQLSAADV--------VLQFTSFAfdvsvweLFWPLINGARLV----IAPPGAHrd 1797
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 338 -ARLGHVVDKHKVSILYTAPTAIRAMMREGDSYVREsnrSSLRLLGSVGEPINPEA---------WNWYYNVVGEGRCPI 407
Cdd:PRK12467 1798 pEQLIQLIERQQVTTLHFVPSMLQQLLQMDEQVEHP---LSLRRVVCGGEALEVEAlrpwlerlpDTGLFNLYGPTETAV 1874
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 408 VDTWW----QTETG------GILIAPLPG---ATALKPgsatrplfgiQPAIVDGE----GnelEGAAEGNLvikdSWPG 470
Cdd:PRK12467 1875 DVTHWtcrrKDLEGrdsvpiGQPIANLSTyilDASLNP----------VPIGVAGElylgG---VGLARGYL----NRPA 1937
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 471 QMRTiwgdpdRFIEAYFSTF-KNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVgmP 549
Cdd:PRK12467 1938 LTAE------RFVADPFGTVgSRLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLREQGGVREAVVI--A 2009
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446925704 550 HDIKG--QGICTFVTLQAGVPESEE----LRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKIAANEL 621
Cdd:PRK12467 2010 QDGANgkQLVAYVVPTDPGLVDDDEaqvaLRAILKNHLKASLPEYMVPAHLVFLARMPLTPNGKLDRKALPAPDASEL 2087
|
|
| A_NRPS_PpsD_like |
cd17650 |
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ... |
106-613 |
2.69e-26 |
|
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341305 [Multi-domain] Cd Length: 447 Bit Score: 112.18 E-value: 2.69e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 106 VSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITad 185
Cdd:cd17650 13 LTYRELNERANQLARTLRGLGVAPGSVVGVCADRSLDAIVGLLAVLKAGGAYVPIDPDYPAERLQYMLEDSGAKLLLT-- 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 186 sslragkllplkenvdlalalpgtecvenvivvyrnanpiemkpgrdlwyhliimevdancppEPmkaEDPLFILYTSGS 265
Cdd:cd17650 91 ---------------------------------------------------------------QP---EDLAYVIYTSGT 104
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 266 TGKPKGVLHTTGGYlVYVASTFKEVFDLKQDDV-YWCTADVGWITGHSYLIYGPLANGTTTLMFEGVPQYPtwARLGHVV 344
Cdd:cd17650 105 TGKPKGVMVEHRNV-AHAAHAWRREYELDSFPVrLLQMASFSFDVFAGDFARSLLNGGTLVICPDEVKLDP--AALYDLI 181
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 345 DKHKVSILYTAPTAIRAMMREgdSYVRESNRSSLRLLgSVGEPINPEAW-NWYYNVVGEgRCPIVDTWWQTETGgILIAP 423
Cdd:cd17650 182 LKSRITLMESTPALIRPVMAY--VYRNGLDLSAMRLL-IVGSDGCKAQDfKTLAARFGQ-GMRIINSYGVTEAT-IDSTY 256
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 424 LPGATALKPGSAT----RPLFGIQPAIVDGEGNELEGAAEGNLVIKDSwpGQMRTIWGDPD----RFIEAYFSTFKNTYF 495
Cdd:cd17650 257 YEEGRDPLGDSANvpigRPLPNTAMYVLDERLQPQPVGVAGELYIGGA--GVARGYLNRPEltaeRFVENPFAPGERMYR 334
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 496 TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVgMPHDIKGQ-GICTFVtLQAGVPESEELR 574
Cdd:cd17650 335 TGDLARWRADGNVELLGRVDHQVKIRGFRIELGEIESQLARHPAIDEAVVA-VREDKGGEaRLCAYV-VAAATLNTAELR 412
|
490 500 510
....*....|....*....|....*....|....*....
gi 446925704 575 KELIswvrKVLGPVASPDALHWAPALPKTRSGKIMRRIL 613
Cdd:cd17650 413 AFLA----KELPSYMIPSYYVQLDALPLTPNGKVDRRAL 447
|
|
| OSB_MenE-like |
cd17630 |
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ... |
255-617 |
2.89e-26 |
|
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.
Pssm-ID: 341285 [Multi-domain] Cd Length: 325 Bit Score: 110.11 E-value: 2.89e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 255 DPLFILYTSGSTGKPKGVLHTTGGyLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLmfeGVPQY 334
Cdd:cd17630 1 RLATVILTSGSTGTPKAVVHTAAN-LLASAAGLHSRLGFGGGDSWLLSLPLYHVGGLAILVRSLLAGAELVL---LERNQ 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 335 PTWARLGHVVDKHkVSIlytAPTAIRAMMREGdsyvreSNRSSLRLLGSV---GEPINPEAwnwyYNVVGEGRCPIVDTW 411
Cdd:cd17630 77 ALAEDLAPPGVTH-VSL---VPTQLQRLLDSG------QGPAALKSLRAVllgGAPIPPEL----LERAADRGIPLYTTY 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 412 WQTETGGILIAPLPGATALkpGSATRPLFGIQPAIVDGEGNELEGAAegnlVIKDSWPGQMRtiwgdPDRFIEAYFSTfk 491
Cdd:cd17630 143 GMTETASQVATKRPDGFGR--GGVGVLLPGRELRIVEDGEIWVGGAS----LAMGYLRGQLV-----PEFNEDGWFTT-- 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 492 ntyftGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESe 571
Cdd:cd17630 210 -----KDLGELHADGRLTVLGRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEELGQRPVAVIVGRGPADPA- 283
|
330 340 350 360
....*....|....*....|....*....|....*....|....*.
gi 446925704 572 elrkELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKIA 617
Cdd:cd17630 284 ----ELRAWLKDKLARFKLPKRIYPVPELPRTGGGKVDRRALRAWL 325
|
|
| PRK07787 |
PRK07787 |
acyl-CoA synthetase; Validated |
247-614 |
4.88e-26 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236096 [Multi-domain] Cd Length: 471 Bit Score: 112.01 E-value: 4.88e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 247 PPEPmKAEDPLFILYTSGSTGKPKGVLHTTGGylvyVASTFKEVFDLKQddvyWCTADV-----------GWITGhsylI 315
Cdd:PRK07787 122 YPEP-DPDAPALIVYTSGTTGPPKGVVLSRRA----IAADLDALAEAWQ----WTADDVlvhglplfhvhGLVLG----V 188
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 316 YGPLANGTT--------------------TLMFeGVPQypTWARLGhvvdkhkvsilyTAPTAIRAMmregdsyvresnr 375
Cdd:PRK07787 189 LGPLRIGNRfvhtgrptpeayaqalseggTLYF-GVPT--VWSRIA------------ADPEAARAL------------- 240
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 376 SSLRLL--GSVGEPInpeawnwyynVVGEGRC-----PIVDTWWQTETGGILIAPLPGATalKPGSATRPLFGIQPAIVD 448
Cdd:PRK07787 241 RGARLLvsGSAALPV----------PVFDRLAaltghRPVERYGMTETLITLSTRADGER--RPGWVGLPLAGVETRLVD 308
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 449 GEGNEL--EGAAEGNLVIKDswPGQMRTIWGDPDRFIEAYfsTFKNTYFTGDGARRDEDGYYWITGRVD-DVLNVSGHRL 525
Cdd:PRK07787 309 EDGGPVphDGETVGELQVRG--PTLFDGYLNRPDATAAAF--TADGWFRTGDVAVVDPDGMHRIVGREStDLIKSGGYRI 384
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 526 GTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESeelrkELISWVRKVLGPVASPDALHWAPALPKTRS 605
Cdd:PRK07787 385 GAGEIETALLGHPGVREAAVVGVPDDDLGQRIVAYVVGADDVAAD-----ELIDFVAQQLSVHKRPREVRFVDALPRNAM 459
|
....*....
gi 446925704 606 GKIMRRILR 614
Cdd:PRK07787 460 GKVLKKQLL 468
|
|
| A_NRPS_Bac |
cd17655 |
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ... |
88-613 |
5.01e-26 |
|
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341310 [Multi-domain] Cd Length: 490 Bit Score: 112.04 E-value: 5.01e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 88 PHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVfggfSPD 167
Cdd:cd17655 11 PDHTAVVFEDQT------LTYRELNERANQLARTLREKGVGPDTIVGIMAERSLEMIVGILGILKAGGAYLPI----DPD 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 168 SLASRI----EDSQAKLVITAdsslragkllplkENVDLALALPGTecvenviVVYRNANPIEMKPGRDLwyhliimevd 243
Cdd:cd17655 81 YPEERIqyilEDSGADILLTQ-------------SHLQPPIAFIGL-------IDLLDEDTIYHEESENL---------- 130
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 244 ancpPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVASTFKEVFDLKQDDV-----YWCTADVGWI-----TGHSY 313
Cdd:cd17655 131 ----EPVSKSDDLAYVIYTSGSTGKPKGVMIEHRGVVNLVEWANKVIYQGEHLRValfasISFDASVTEIfasllSGNTL 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 314 LIYGplanGTTTLMFEGVPQYptwarlghvVDKHKVSILYTAPTAIRAMmregdSYVRESNRSSLRLLGSVGEPINPE-A 392
Cdd:cd17655 207 YIVR----KETVLDGQALTQY---------IRQNRITIIDLTPAHLKLL-----DAADDSEGLSLKHLIVGGEALSTElA 268
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 393 WNW---------YYNVVGEGRCPIVDTWWQTETGGILIAPLP-GatalkpgsatRPLFGIQPAIVDGEGNELEGAAEGNL 462
Cdd:cd17655 269 KKIielfgtnptITNAYGPTETTVDASIYQYEPETDQQVSVPiG----------KPLGNTRIYILDQYGRPQPVGVAGEL 338
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 463 VIkdSWPGQMRTIWGDPD----RFIEAYFSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHE 538
Cdd:cd17655 339 YI--GGEGVARGYLNRPEltaeKFVDDPFVPGERMYRTGDLARWLPDGNIEFLGRIDHQVKIRGYRIELGEIEARLLQHP 416
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 539 AVAEAAVVGMPHDIKGQGICTFVTLQAGVPeSEELRKELiswvRKVLgpvasPDalHWAPA-------LPKTRSGKIMRR 611
Cdd:cd17655 417 DIKEAVVIARKDEQGQNYLCAYIVSEKELP-VAQLREFL----AREL-----PD--YMIPSyfikldeIPLTPNGKVDRK 484
|
..
gi 446925704 612 IL 613
Cdd:cd17655 485 AL 486
|
|
| Firefly_Luc |
cd17642 |
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ... |
106-616 |
6.69e-26 |
|
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341297 [Multi-domain] Cd Length: 532 Bit Score: 112.24 E-value: 6.69e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 106 VSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLV---- 181
Cdd:cd17642 45 YSYAEYLEMSVRLAEALKKYGLKQNDRIAVCSENSLQFFLPVIAGLFIGVGVAPTNDIYNERELDHSLNISKPTIVfcsk 124
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 182 ITADSSLRAGKLLPLKENV---DLALALPGTECVENVIVVYRNANpiemkpgrdlwyhliiMEVDANCPPEPMKAEDPLF 258
Cdd:cd17642 125 KGLQKVLNVQKKLKIIKTIiilDSKEDYKGYQCLYTFITQNLPPG----------------FNEYDFKPPSFDRDEQVAL 188
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 259 ILYTSGSTGKPKGVLHTTGGYLVYVASTFKEVF-DLKQDDVYWCTAdVGWItgHSYLIYGPLanGTTTLMFEGVPQYPTW 337
Cdd:cd17642 189 IMNSSGSTGLPKGVQLTHKNIVARFSHARDPIFgNQIIPDTAILTV-IPFH--HGFGMFTTL--GYLICGFRVVLMYKFE 263
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 338 ARLG-HVVDKHKVSILYTAPTAIRAMMREgdSYVRESNRSSLRLLGSVGEPINPEawnwyynvVGEG-----RCPIV-DT 410
Cdd:cd17642 264 EELFlRSLQDYKVQSALLVPTLFAFFAKS--TLVDKYDLSNLHEIASGGAPLSKE--------VGEAvakrfKLPGIrQG 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 411 WWQTE-TGGILIAPlpgATALKPGSATRPLFGIQPAIVDGEGNELEGAAE-GNLVIKDswPGQMRTIWGDPDRFIEAyfs 488
Cdd:cd17642 334 YGLTEtTSAILITP---EGDDKPGAVGKVVPFFYAKVVDLDTGKTLGPNErGELCVKG--PMIMKGYVNNPEATKAL--- 405
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 489 TFKNTYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGV 567
Cdd:cd17642 406 IDKDGWLhSGDIAYYDEDGHFFIVDRLKSLIKYKGYQVPPAELESILLQHPKIFDAGVAGIPDEDAGELPAAVVVLEAGK 485
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 446925704 568 PESEelrKELISWV-------RKVLGPVASPDalhwapALPKTRSGKIMRRILRKI 616
Cdd:cd17642 486 TMTE---KEVMDYVasqvstaKRLRGGVKFVD------EVPKGLTGKIDRRKIREI 532
|
|
| PRK08162 |
PRK08162 |
acyl-CoA synthetase; Validated |
80-620 |
1.02e-25 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236169 [Multi-domain] Cd Length: 545 Bit Score: 111.58 E-value: 1.02e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIwegdHPSRHkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCV 159
Cdd:PRK08162 24 LERAAEVYPDRPAVI----HGDRR--RTWAETYARCRRLASALARRGIGRGDTVAVLLPNIPAMVEAHFGVPMAGAVLNT 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFGGFSPDSLASRIEDSQAKLVIT----ADSSLRAGKLLPLKENVDLALALPgtecvenvivVYRNANPIemkpgRDLWY 235
Cdd:PRK08162 98 LNTRLDAASIAFMLRHGEAKVLIVdtefAEVAREALALLPGPKPLVIDVDDP----------EYPGGRFI-----GALDY 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 236 HLIIMEVDANCPPEPMKAE-DPLFILYTSGSTGKPKGVL-HTTGGYLVYVASTFkeVFDLKQDDVY-----------WCT 302
Cdd:PRK08162 163 EAFLASGDPDFAWTLPADEwDAIALNYTSGTTGNPKGVVyHHRGAYLNALSNIL--AWGMPKHPVYlwtlpmfhcngWCF 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 303 AdvgWitghsyliygplangTTTLMfEGVPQYPTWARLGHVVD---KHKVSILYTAPTaIRAMMREGDSYVRESNRSSLR 379
Cdd:PRK08162 241 P---W---------------TVAAR-AGTNVCLRKVDPKLIFDlirEHGVTHYCGAPI-VLSALINAPAEWRAGIDHPVH 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 380 LLGSVGEPinPEAwnwyynVVG----------------EGRCPIVDTWWQTETG--------------GILIAPLPGATA 429
Cdd:PRK08162 301 AMVAGAAP--PAA------VIAkmeeigfdlthvygltETYGPATVCAWQPEWDalplderaqlkarqGVRYPLQEGVTV 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 430 LKPGSatrplfgIQPAIVDGE--GnelEGAAEGNLVIKdswpGQMRtiwgDPDRFIEAyfstFKNTYF-TGDGARRDEDG 506
Cdd:PRK08162 373 LDPDT-------MQPVPADGEtiG---EIMFRGNIVMK----GYLK----NPKATEEA----FAGGWFhTGDLAVLHPDG 430
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 507 YYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEElrkELISWVRKVLG 586
Cdd:PRK08162 431 YIKIKDRSKDIIISGGENISSIEVEDVLYRHPAVLVAAVVAKPDPKWGEVPCAFVELKDGASATEE---EIIAHCREHLA 507
|
570 580 590
....*....|....*....|....*....|....
gi 446925704 587 PVASPDALHWAPaLPKTRSGKIMRRILRKIAANE 620
Cdd:PRK08162 508 GFKVPKAVVFGE-LPKTSTGKIQKFVLREQAKSL 540
|
|
| PRK05857 |
PRK05857 |
fatty acid--CoA ligase; |
74-613 |
1.14e-25 |
|
fatty acid--CoA ligase;
Pssm-ID: 180293 [Multi-domain] Cd Length: 540 Bit Score: 111.64 E-value: 1.14e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 74 NVSANCLDRHLKEHPHKPAIIW--EGDHPSRhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACA 151
Cdd:PRK05857 11 QLPSTVLDRVFEQARQQPEAIAlrRCDGTSA---LRYRELVAEVGGLAADLRAQSVSRGSRVLVISDNGPETYLSVLACA 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 152 RIGAVHCVVFGGFSPDSLASRIEDSQAKLVITADSSLRAGKLLPlkenvDLALALPGTECVENVIVVYRNANPIEMKPGR 231
Cdd:PRK05857 88 KLGAIAVMADGNLPIAAIERFCQITDPAAALVAPGSKMASSAVP-----EALHSIPVIAVDIAAVTRESEHSLDAASLAG 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 232 DLWYhliimevdancppepmKAEDPLFILYTSGSTGKPKGVLHTTggylvyvaSTFKEVFD-LKQDDVYWctadVGWITG 310
Cdd:PRK05857 163 NADQ----------------GSEDPLAMIFTSGTTGEPKAVLLAN--------RTFFAVPDiLQKEGLNW----VTWVVG 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 311 HSylIYGPLANG--------TTTLMFEG--VPQYPTWARLGHVVDKHKVSILYTAPTAIRAMMRE---GDSYVresnrSS 377
Cdd:PRK05857 215 ET--TYSPLPAThigglwwiLTCLMHGGlcVTGGENTTSLLEILTTNAVATTCLVPTLLSKLVSElksANATV-----PS 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 378 LRLLGsvgepinpeawnwyynvVGEGRCPIVDTWWqTETGGILIAPL-----PGATAL------------KPGSATRPLF 440
Cdd:PRK05857 288 LRLVG-----------------YGGSRAIAADVRF-IEATGVRTAQVyglseTGCTALclptddgsivkiEAGAVGRPYP 349
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 441 GIQP--AIVDGEGNELEGAAE----GNLVIKDswPGQMRTIWGDPDRFIEAYFSTFKNTyftGDGARRDEDGYYWITGRV 514
Cdd:PRK05857 350 GVDVylAATDGIGPTAPGAGPsasfGTLWIKS--PANMLGYWNNPERTAEVLIDGWVNT---GDLLERREDGFFYIKGRS 424
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 515 DDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQ--GICTFVTLQAGVPESEELRKELISWVRKVLGPVASPD 592
Cdd:PRK05857 425 SEMIICGGVNIAPDEVDRIAEGVSGVREAACYEIPDEEFGAlvGLAVVASAELDESAARALKHTIAARFRRESEPMARPS 504
|
570 580
....*....|....*....|.
gi 446925704 593 ALHWAPALPKTRSGKIMRRIL 613
Cdd:PRK05857 505 TIVIVTDIPRTQSGKVMRASL 525
|
|
| PLN02330 |
PLN02330 |
4-coumarate--CoA ligase-like 1 |
104-615 |
5.13e-25 |
|
4-coumarate--CoA ligase-like 1
Pssm-ID: 215189 [Multi-domain] Cd Length: 546 Bit Score: 109.68 E-value: 5.13e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 104 KIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVhcvvFGGFSPDSLASRI----EDSQAK 179
Cdd:PLN02330 54 KAVTYGEVVRDTRRFAKALRSLGLRKGQVVVVVLPNVAEYGIVALGIMAAGGV----FSGANPTALESEIkkqaEAAGAK 129
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 180 LVITADssLRAGKLlplkENVDLALALPGTECVENVIvvyrNANPIEMKPGR--DLWYHLIIMEVDANCPPepmkaedpl 257
Cdd:PLN02330 130 LIVTND--TNYGKV----KGLGLPVIVLGEEKIEGAV----NWKELLEAADRagDTSDNEEILQTDLCALP--------- 190
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 258 filYTSGSTGKPKGVLHTTGGYLVYVASTFKEVfdlkQDDVYWCTADVGWITghSYLIYGPLANGTTTLMFEG----VPQ 333
Cdd:PLN02330 191 ---FSSGTTGISKGVMLTHRNLVANLCSSLFSV----GPEMIGQVVTLGLIP--FFHIYGITGICCATLRNKGkvvvMSR 261
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 334 YPTWARLgHVVDKHKVSILYTAPTAIRAMMRegDSYVRESNRSSLRL--LGSVGEPINPEAWNWYynvvgEGRCPIV--- 408
Cdd:PLN02330 262 FELRTFL-NALITQEVSFAPIVPPIILNLVK--NPIVEEFDLSKLKLqaIMTAAAPLAPELLTAF-----EAKFPGVqvq 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 409 DTWWQTETGGILIA---PLPGATALKPGSATRPLFGIQPAIVDGE-GNELEGAAEGNLVIKDSWPGQ---------MRTI 475
Cdd:PLN02330 334 EAYGLTEHSCITLThgdPEKGHGIAKKNSVGFILPNLEVKFIDPDtGRSLPKNTPGELCVRSQCVMQgyynnkeetDRTI 413
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 476 wgDPDRFIEayfstfkntyfTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQ 555
Cdd:PLN02330 414 --DEDGWLH-----------TGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDAAVVPLPDEEAGE 480
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 556 GICTFVTLQAGVPESEElrkELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRK 615
Cdd:PLN02330 481 IPAACVVINPKAKESEE---DILNFVAANVAHYKKVRVVQFVDSIPKSLSGKIMRRLLKE 537
|
|
| PRK08974 |
PRK08974 |
long-chain-fatty-acid--CoA ligase FadD; |
80-614 |
9.17e-25 |
|
long-chain-fatty-acid--CoA ligase FadD;
Pssm-ID: 236359 [Multi-domain] Cd Length: 560 Bit Score: 108.99 E-value: 9.17e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIWEGdhpsrhKIVSYKELHDEVCRFANVLK-KYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHC 158
Cdd:PRK08974 29 FEQAVARYADQPAFINMG------EVMTFRKLEERSRAFAAYLQnGLGLKKGDRVALMMPNLLQYPIALFGILRAGMIVV 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 159 VVFGGFSPDSLASRIEDSQAK-LVITAD--SSL---------------RAGKLLPLKEN--VDLALA----------LPG 208
Cdd:PRK08974 103 NVNPLYTPRELEHQLNDSGAKaIVIVSNfaHTLekvvfktpvkhviltRMGDQLSTAKGtlVNFVVKyikrlvpkyhLPD 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 209 TecvenviVVYRNAnpieMKPGRDLWYhliimevdanCPPEpMKAEDPLFILYTSGSTGKPKGVLHTTGGYLVyvastfk 288
Cdd:PRK08974 183 A-------ISFRSA----LHKGRRMQY----------VKPE-LVPEDLAFLQYTGGTTGVAKGAMLTHRNMLA------- 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 289 evfDLKQDDVywctadvgwitghsylIYGPLANGTTTLMFEGVPQYptwarlgHV----------VDKHKVSILYTAPTA 358
Cdd:PRK08974 234 ---NLEQAKA----------------AYGPLLHPGKELVVTALPLY-------HIfaltvncllfIELGGQNLLITNPRD 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 359 IRAMMREGDSY--------------------VRESNRSSLRLlgSVGepinpeawnwyynvvgeGRCPI---VDTWWQTE 415
Cdd:PRK08974 288 IPGFVKELKKYpftaitgvntlfnallnneeFQELDFSSLKL--SVG-----------------GGMAVqqaVAERWVKL 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 416 TGGILI--------APL----PGATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDswPGQMRTIWGDPdrfi 483
Cdd:PRK08974 349 TGQYLLegygltecSPLvsvnPYDLDYYSGSIGLPVPSTEIKLVDDDGNEVPPGEPGELWVKG--PQVMLGYWQRP---- 422
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 484 EAYFSTFKNTYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVt 562
Cdd:PRK08974 423 EATDEVIKDGWLaTGDIAVMDEEGFLRIVDRKKDMILVSGFNVYPNEIEDVVMLHPKVLEVAAVGVPSEVSGEAVKIFV- 501
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|...
gi 446925704 563 lqagVPESEEL-RKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILR 614
Cdd:PRK08974 502 ----VKKDPSLtEEELITHCRRHLTGYKVPKLVEFRDELPKSNVGKILRRELR 550
|
|
| A_NRPS_SidN3_like |
cd05918 |
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ... |
82-619 |
1.30e-24 |
|
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341242 [Multi-domain] Cd Length: 481 Bit Score: 107.63 E-value: 1.30e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 82 RHLKEHPHKPAI-IWEGDhpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVhCVV 160
Cdd:cd05918 7 ERARSQPDAPAVcAWDGS-------LTYAELDRLSSRLAHHLRSLGVGPGVFVPLCFEKSKWAVVAMLAVLKAGGA-FVP 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 161 FGGFSPDS-LASRIEDSQAKLVITADSSlragkllplkenvdlalalpgtecvenvivvyrnanpiemkpgrdlwyhlii 239
Cdd:cd05918 79 LDPSHPLQrLQEILQDTGAKVVLTSSPS---------------------------------------------------- 106
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 240 mevdancppepmkaeDPLFILYTSGSTGKPKGVL--HTTggyLVYVASTFKEVFDLKQDD-VYWCTA---DVgwitgHSY 313
Cdd:cd05918 107 ---------------DAAYVIFTSGSTGKPKGVVieHRA---LSTSALAHGRALGLTSESrVLQFASytfDV-----SIL 163
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 314 LIYGPLANGTTTLmfegVP-QYPTWARLGHVVDKHKVSILYTAPTAIRAMMREGDsyvresnrSSLRLLGSVGEPINPEa 392
Cdd:cd05918 164 EIFTTLAAGGCLC----IPsEEDRLNDLAGFINRLRVTWAFLTPSVARLLDPEDV--------PSLRTLVLGGEALTQS- 230
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 393 wnwyynvvgegrcpIVDTWWQ----------TET--GGILIAPLPGATALKPGSAtrplFGIQPAIVD-GEGNEL--EGA 457
Cdd:cd05918 231 --------------DVDTWADrvrlinaygpAECtiAATVSPVVPSTDPRNIGRP----LGATCWVVDpDNHDRLvpIGA 292
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 458 AeGNLVIkdSWPGQMRTIWGDPDR----FIEAYFSTFKNT-------YFTGDGARRDEDG--YYwiTGRVDDVLNVSGHR 524
Cdd:cd05918 293 V-GELLI--EGPILARGYLNDPEKtaaaFIEDPAWLKQEGsgrgrrlYRTGDLVRYNPDGslEY--VGRKDTQVKIRGQR 367
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 525 LGTAEIESALVSHEAVAEAAVVGM--PHDIKGQGI-CTFVTLQ-----------AGVPESEELR---KELISWVRKVLGP 587
Cdd:cd05918 368 VELGEIEHHLRQSLPGAKEVVVEVvkPKDGSSSPQlVAFVVLDgsssgsgdgdsLFLEPSDEFRalvAELRSKLRQRLPS 447
|
570 580 590
....*....|....*....|....*....|....
gi 446925704 588 VASPDalHWAP--ALPKTRSGKIMRRILRKIAAN 619
Cdd:cd05918 448 YMVPS--VFLPlsHLPLTASGKIDRRALRELAES 479
|
|
| FACL_like_2 |
cd05917 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
254-614 |
1.93e-24 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341241 [Multi-domain] Cd Length: 349 Bit Score: 105.05 E-value: 1.93e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 254 EDPLFILYTSGSTGKPKGVLHT-----TGGYLVyvastfKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMF 328
Cdd:cd05917 2 DDVINIQFTSGTTGSPKGATLThhnivNNGYFI------GERLGLTEQDRLCIPVPLFHCFGSVLGVLACLTHGATMVFP 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 329 EgvPQYPTWARLgHVVDKHKVSILYTAPTAIRAMMREGDsyVRESNRSSLRLLGSVGEPINPEAWNWYYNVVGEGRCPIV 408
Cdd:cd05917 76 S--PSFDPLAVL-EAIEKEKCTALHGVPTMFIAELEHPD--FDKFDLSSLRTGIMAGAPCPPELMKRVIEVMNMKDVTIA 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 409 dtWWQTETGGILIAPLPGATA-LKPGSATRPLFGIQPAIVDGEGNELEG-AAEGNLVIKDSwpGQMRTIWGDPDRFIEAY 486
Cdd:cd05917 151 --YGMTETSPVSTQTRTDDSIeKRVNTVGRIMPHTEAKIVDPEGGIVPPvGVPGELCIRGY--SVMKGYWNDPEKTAEAI 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 487 fsTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAG 566
Cdd:cd05917 227 --DGDGWLHTGDLAVMDEDGYCRIVGRIKDMIIRGGENIYPREIEEFLHTHPKVSDVQVVGVPDERYGEEVCAWIRLKEG 304
|
330 340 350 360
....*....|....*....|....*....|....*....|....*...
gi 446925704 567 VPESEElrkELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILR 614
Cdd:cd05917 305 AELTEE---DIKAYCKGKIAHYKVPRYVFFVDEFPLTVSGKIQKFKLR 349
|
|
| PLN02574 |
PLN02574 |
4-coumarate--CoA ligase-like |
69-623 |
2.14e-24 |
|
4-coumarate--CoA ligase-like
Pssm-ID: 215312 [Multi-domain] Cd Length: 560 Bit Score: 107.62 E-value: 2.14e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 69 ADGELNVSANCLDRHlkEHPHKPAIIwegDHPSRHKIvSYKELHDEVCRFANVLKKY-GIGKGDRVVLYMPMVTEAAIAM 147
Cdd:PLN02574 36 SDPNLDAVSFIFSHH--NHNGDTALI---DSSTGFSI-SYSELQPLVKSMAAGLYHVmGVRQGDVVLLLLPNSVYFPVIF 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 148 LACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITADSSLraGKLLPLKenvdlalaLPgtecvenVIVVYRNANPIEM 227
Cdd:PLN02574 110 LAVLSLGGIVTTMNPSSSLGEIKKRVVDCSVGLAFTSPENV--EKLSPLG--------VP-------VIGVPENYDFDSK 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 228 KPGRDLWYHLIIMEVDAnCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVASTFKevFDLKQ------DDVYWC 301
Cdd:PLN02574 173 RIEFPKFYELIKEDFDF-VPKPVIKQDDVAAIMYSSGTTGASKGVVLTHRNLIAMVELFVR--FEASQyeypgsDNVYLA 249
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 302 TADVGWITGHSYLIYGPLANGTTTLMFEGVPQyptwARLGHVVDKHKVSILYTAPTAIRAMMREGDSYVRESNRSsLRLL 381
Cdd:PLN02574 250 ALPMFHIYGLSLFVVGLLSLGSTIVVMRRFDA----SDMVKVIDRFKVTHFPVVPPILMALTKKAKGVCGEVLKS-LKQV 324
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 382 GSVGEPINPEAWNWYYNVVgegrcPIVD---TWWQTETGGILIAPLPGATALKPGSATRPLFGIQPAIVDGE-GNELEGA 457
Cdd:PLN02574 325 SCGAAPLSGKFIQDFVQTL-----PHVDfiqGYGMTESTAVGTRGFNTEKLSKYSSVGLLAPNMQAKVVDWStGCLLPPG 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 458 AEGNLVIKDswPGQMRTIWGDPdrfiEAYFSTFKNTYF--TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALV 535
Cdd:PLN02574 400 NCGELWIQG--PGVMKGYLNNP----KATQSTIDKDGWlrTGDIAYFDEDGYLYIVDRLKEIIKYKGFQIAPADLEAVLI 473
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 536 SHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEElrkELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRK 615
Cdd:PLN02574 474 SHPEIIDAAVTAVPDKECGEIPVAFVVRRQGSTLSQE---AVINYVAKQVAPYKKVRKVVFVQSIPKSPAGKILRRELKR 550
|
....*...
gi 446925704 616 IAANELDS 623
Cdd:PLN02574 551 SLTNSVSS 558
|
|
| BACL_like |
cd05929 |
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ... |
259-614 |
2.33e-24 |
|
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.
Pssm-ID: 341252 [Multi-domain] Cd Length: 473 Bit Score: 106.69 E-value: 2.33e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 259 ILYTSGSTGKPKGVLHTTGGYLVYVAS--TFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFEGVPQypT 336
Cdd:cd05929 130 MLYSGGTTGRPKGIKRGLPGGPPDNDTlmAAALGFGPGADSVYLSPAPLYHAAPFRWSMTALFMGGTLVLMEKFDPE--E 207
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 337 WARLghvVDKHKVSILYTAPTAIRAMMREGDSYVRESNRSSLRLLGSVGEPINPEA------------WNWYYNVVGEGR 404
Cdd:cd05929 208 FLRL---IERYRVTFAQFVPTMFVRLLKLPEAVRNAYDLSSLKRVIHAAAPCPPWVkeqwidwggpiiWEYYGGTEGQGL 284
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 405 CPIVDTWWQTEtggiliaplpgatalkPGSATRPLFGiQPAIVDGEGNELEGAAEGNLVIKDSWPgqmRTIWGDPDRFIE 484
Cdd:cd05929 285 TIINGEEWLTH----------------PGSVGRAVLG-KVHILDEDGNEVPPGEIGEVYFANGPG---FEYTNDPEKTAA 344
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 485 AY----FSTFkntyftGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTF 560
Cdd:cd05929 345 ARneggWSTL------GDVGYLDEDGYLYLTDRRSDMIISGGVNIYPQEIENALIAHPKVLDAAVVGVPDEELGQRVHAV 418
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....
gi 446925704 561 VTLQAGVPESEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILR 614
Cdd:cd05929 419 VQPAPGADAGTALAEELIAFLRDRLSRYKCPRSIEFVAELPRDDTGKLYRRLLR 472
|
|
| PRK07867 |
PRK07867 |
acyl-CoA synthetase; Validated |
234-615 |
2.33e-24 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236120 [Multi-domain] Cd Length: 529 Bit Score: 107.46 E-value: 2.33e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 234 WYHLIIMEVDANCPPEPMKAEDPLFILYTSGSTGKPKGVLhTTGGYLVYVASTFKEVFDLKQDDVYWCTAD--------V 305
Cdd:PRK07867 132 WADELAAHRDAEPPFRVADPDDLFMLIFTSGTSGDPKAVR-CTHRKVASAGVMLAQRFGLGPDDVCYVSMPlfhsnavmA 210
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 306 GWITGhsyliygpLANGTTTLM---FEGVPQYPTWARLG----HVVDKHKVSILYTAPtairammREGDSyvresnRSSL 378
Cdd:PRK07867 211 GWAVA--------LAAGASIALrrkFSASGFLPDVRRYGatyaNYVGKPLSYVLATPE-------RPDDA------DNPL 269
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 379 RLL-GSVGEPINPEAWnwyynvvgEGR--CPIVDTWWQTEtGGILIAPLPGAtalKPGSATRPLFGIQ-----------P 444
Cdd:PRK07867 270 RIVyGNEGAPGDIARF--------ARRfgCVVVDGFGSTE-GGVAITRTPDT---PPGALGPLPPGVAivdpdtgtecpP 337
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 445 AIVDGEGNELEGAAEGNLVIKDSwPGQMRTIWGDPdrfiEAYFSTFKN-TYFTGDGARRDEDGYYWITGRVDDVLNVSGH 523
Cdd:PRK07867 338 AEDADGRLLNADEAIGELVNTAG-PGGFEGYYNDP----EADAERMRGgVYWSGDLAYRDADGYAYFAGRLGDWMRVDGE 412
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 524 RLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVP-ESEELRKELISwvRKVLGPVASPDALHWAPALPK 602
Cdd:PRK07867 413 NLGTAPIERILLRYPDATEVAVYAVPDPVVGDQVMAALVLAPGAKfDPDAFAEFLAA--QPDLGPKQWPSYVRVCAELPR 490
|
410
....*....|...
gi 446925704 603 TRSGKIMRRILRK 615
Cdd:PRK07867 491 TATFKVLKRQLSA 503
|
|
| ACLS-CaiC |
cd17637 |
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ... |
255-610 |
2.47e-24 |
|
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341292 [Multi-domain] Cd Length: 333 Bit Score: 104.27 E-value: 2.47e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 255 DPLFILYTSGSTGKPKGVLHTTGGyLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFEGVPQy 334
Cdd:cd17637 1 DPFVIIHTAAVAGRPRGAVLSHGN-LIAANLQLIHAMGLTEADVYLNMLPLFHIAGLNLALATFHAGGANVVMEKFDPA- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 335 ptwaRLGHVVDKHKVSILYTAPTAIRAMMREGDSyvRESNRSSLRLLGSVGEPINPEAWnwyynvvgEGRCPivDTWW-- 412
Cdd:cd17637 79 ----EALELIEEEKVTLMGSFPPILSNLLDAAEK--SGVDLSSLRHVLGLDAPETIQRF--------EETTG--ATFWsl 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 413 --QTETGG-ILIAPlpgaTALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDswPGQMRTIWGDPDrfIEAYfsT 489
Cdd:cd17637 143 ygQTETSGlVTLSP----YRERPGSAGRPGPLVRVRIVDDNDRPVPAGETGEIVVRG--PLVFQGYWNLPE--LTAY--T 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 490 FKNTYF-TGDGARRDEDGYYWITGRV--DDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPhDIK-GQGICTFVTLQA 565
Cdd:cd17637 213 FRNGWHhTGDLGRFDEDGYLWYAGRKpeKELIKPGGENVYPAEVEKVILEHPAIAEVCVIGVP-DPKwGEGIKAVCVLKP 291
|
330 340 350 360
....*....|....*....|....*....|....*....|....*
gi 446925704 566 GVPESEElrkELISWVRKVLGPVASPDALHWAPALPKTRSGKIMR 610
Cdd:cd17637 292 GATLTAD---ELIEFVGSRIARYKKPRYVVFVEALPKTADGSIDR 333
|
|
| FAAL |
cd05931 |
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ... |
82-615 |
2.47e-24 |
|
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.
Pssm-ID: 341254 [Multi-domain] Cd Length: 547 Bit Score: 107.32 E-value: 2.47e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 82 RHLKEHPHKPAIIWEGDHPSRHKIVSYKELHDEVCRFANVLKKYGiGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVF 161
Cdd:cd05931 1 RRAAARPDRPAYTFLDDEGGREETLTYAELDRRARAIAARLQAVG-KPGDRVLLLAPPGLDFVAAFLGCLYAGAIAVPLP 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 162 ---GGFSPDSLASRIEDSQAKLVITADSSLRAgklLPLKENVDLALALPGTECVENVivvyrnanpieMKPGRDLWyhli 238
Cdd:cd05931 80 pptPGRHAERLAAILADAGPRVVLTTAAALAA---VRAFAASRPAAGTPRLLVVDLL-----------PDTSAADW---- 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 239 imevdanCPPEPmKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVASTFKeVFDLKQDDVYwctadVGWI-TGHSY-LIY 316
Cdd:cd05931 142 -------PPPSP-DPDDIAYLQYTSGSTGTPKGVVVTHRNLLANVRQIRR-AYGLDPGDVV-----VSWLpLYHDMgLIG 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 317 G---PLANGTTTLMFEgvpqyPT--------WARLghvVDKHKVSILYtAPT-----AIRAMMREGdsyVRESNRSSLRL 380
Cdd:cd05931 208 GlltPLYSGGPSVLMS-----PAaflrrplrWLRL---ISRYRATISA-APNfaydlCVRRVRDED---LEGLDLSSWRV 275
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 381 LGSVGEPINPEAWNWYYN--------------------------VVGEGRCPIVDTWWQTETGGILIAPL---PGATALK 431
Cdd:cd05931 276 ALNGAEPVRPATLRRFAEafapfgfrpeafrpsyglaeatlfvsGGPPGTGPVVLRVDRDALAGRAVAVAaddPAARELV 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 432 pgSATRPLFGIQPAIVDGEGN-ELEGAAEGNLVIKDswPGQMRTIWGDPDrFIEAYFSTFKNT----YF-TGD-GARRde 504
Cdd:cd05931 356 --SCGRPLPDQEVRIVDPETGrELPDGEVGEIWVRG--PSVASGYWGRPE-ATAETFGALAATdeggWLrTGDlGFLH-- 428
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 505 DGYYWITGRVDDVLNVSGHRLGTAEIE-SALVSHEAVAE--AAVVGMPHDIKGQGIctfVTLQAGVPESEELRKELISWV 581
Cdd:cd05931 429 DGELYITGRLKDLIIVRGRNHYPQDIEaTAEEAHPALRPgcVAAFSVPDDGEERLV---VVAEVERGADPADLAAIAAAI 505
|
570 580 590 600
....*....|....*....|....*....|....*....|.
gi 446925704 582 RKVlgpVAS-----PDALHWAP--ALPKTRSGKIMRRILRK 615
Cdd:cd05931 506 RAA---VARehgvaPADVVLVRpgSIPRTSSGKIQRRACRA 543
|
|
| entF |
PRK10252 |
enterobactin non-ribosomal peptide synthetase EntF; |
80-613 |
2.72e-24 |
|
enterobactin non-ribosomal peptide synthetase EntF;
Pssm-ID: 236668 [Multi-domain] Cd Length: 1296 Bit Score: 108.98 E-value: 2.72e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCV 159
Cdd:PRK10252 464 VAQQAAKTPDAPALADARYQ------FSYREMREQVVALANLLRERGVKPGDSVAVALPRSVFLTLALHAIVEAGAAWLP 537
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFGGFSPDSLASRIEDSQAKLVITADsslragkllplkenvDLALALPGTEcvenVIVVYRNANPIEMKPGRDLwyhlii 239
Cdd:PRK10252 538 LDTGYPDDRLKMMLEDARPSLLITTA---------------DQLPRFADVP----DLTSLCYNAPLAPQGAAPL------ 592
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 240 mevdanCPPEPmkaEDPLFILYTSGSTGKPKGVL--HTTggyLVYVASTFKEVFDLKQDDVYW----CTADVG-Witghs 312
Cdd:PRK10252 593 ------QLSQP---HHTAYIIFTSGSTGRPKGVMvgQTA---IVNRLLWMQNHYPLTADDVVLqktpCSFDVSvW----- 655
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 313 yLIYGPLANGTTTLMFE-GVPQYPTWarLGHVVDKHKVSILYTAPTAIRAMMREGDSYVRESNRSSLRLLGSVGEPINPE 391
Cdd:PRK10252 656 -EFFWPFIAGAKLVMAEpEAHRDPLA--MQQFFAEYGVTTTHFVPSMLAAFVASLTPEGARQSCASLRQVFCSGEALPAD 732
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 392 AWN-WY-------YNVVGEGRCPIVDTWWqtETGGILIAPLPGAtalkPGSATRPLFGIQPAIVDGEGNELEGAAEGNLV 463
Cdd:PRK10252 733 LCReWQqltgaplHNLYGPTEAAVDVSWY--PAFGEELAAVRGS----SVPIGYPVWNTGLRILDARMRPVPPGVAGDLY 806
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 464 IKdswpG-QM-RTIWGDPD----RFIEAYFSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSH 537
Cdd:PRK10252 807 LT----GiQLaQGYLGRPDltasRFIADPFAPGERMYRTGDVARWLDDGAVEYLGRSDDQLKIRGQRIELGEIDRAMQAL 882
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 538 EAVAEAAVV------GMPHDIKGQGICTFVTLQAGVP-ESEELRKELiswvRKVLGPVASPDALHWAPALPKTRSGKIMR 610
Cdd:PRK10252 883 PDVEQAVTHacvinqAAATGGDARQLVGYLVSQSGLPlDTSALQAQL----RERLPPHMVPVVLLQLDQLPLSANGKLDR 958
|
...
gi 446925704 611 RIL 613
Cdd:PRK10252 959 KAL 961
|
|
| LC_FACS_bac |
cd05932 |
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ... |
107-588 |
1.14e-23 |
|
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341255 [Multi-domain] Cd Length: 508 Bit Score: 105.24 E-value: 1.14e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 107 SYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITads 186
Cdd:cd05932 8 TWGEVADKARRLAAALRALGLEPGSKIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRYVLEHSESKALFV--- 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 187 slraGKLlplKENVDLALALPGTecvenviVVYRNANPIEMKPGRDLWYHLIIMEvdancPPE----PMKAEDPLFILYT 262
Cdd:cd05932 85 ----GKL---DDWKAMAPGVPEG-------LISISLPPPSAANCQYQWDDLIAQH-----PPLeerpTRFPEQLATLIYT 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 263 SGSTGKPKGVLHTTGGYlVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFEGVPQY-------- 334
Cdd:cd05932 146 SGTTGQPKGVMLTFGSF-AWAAQAGIEHIGTEENDRMLSYLPLAHVTERVFVEGGSLYGGVLVAFAESLDTFvedvqrar 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 335 PT--------WARLGH-VVDK---HKVSILYTAPTAIRAMMREgdsYVRESNRSSLRLLGSVGEPINPEAWNWYYNVvge 402
Cdd:cd05932 225 PTlffsvprlWTKFQQgVQDKipqQKLNLLLKIPVVNSLVKRK---VLKGLGLDQCRLAGCGSAPVPPALLEWYRSL--- 298
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 403 gRCPIVDTWWQTETGGILIAPLPGATalKPGSATRPLFGIQPAIVDgegnelegaaEGNLVIKDswPGQMRTIWGDPDRF 482
Cdd:cd05932 299 -GLNILEAYGMTENFAYSHLNYPGRD--KIGTVGNAGPGVEVRISE----------DGEILVRS--PALMMGYYKDPEAT 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 483 IEAYfsTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVS-GHRLGTAEIESALVSHEAVAEAAVVG--MPHDIkgqGICt 559
Cdd:cd05932 364 AEAF--TADGFLRTGDKGELDADGNLTITGRVKDIFKTSkGKYVAPAPIENKLAEHDRVEMVCVIGsgLPAPL---ALV- 437
|
490 500 510
....*....|....*....|....*....|
gi 446925704 560 fVTLQAGVPESEE-LRKELISWVRKVLGPV 588
Cdd:cd05932 438 -VLSEEARLRADAfARAELEASLRAHLARV 466
|
|
| PLN03102 |
PLN03102 |
acyl-activating enzyme; Provisional |
99-617 |
1.93e-23 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215576 [Multi-domain] Cd Length: 579 Bit Score: 104.72 E-value: 1.93e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 99 HPSRHKIV------SYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASR 172
Cdd:PLN03102 27 YPNRTSIIygktrfTWPQTYDRCCRLAASLISLNITKNDVVSVLAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAI 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 173 IEDSQAKLVITADSslragkLLPLKENVdLALaLPGTECVENVIVVYRNANPIEMKP-GRDLWYHLIIMEVDancpPEPM 251
Cdd:PLN03102 107 LRHAKPKILFVDRS------FEPLAREV-LHL-LSSEDSNLNLPVIFIHEIDFPKRPsSEELDYECLIQRGE----PTPS 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 252 ---------KAEDPLFILYTSGSTGKPKGVLHT-TGGYLVYVASTFKevFDLKQDDVYWCTADV----GWItghsyLIYG 317
Cdd:PLN03102 175 lvarmfriqDEHDPISLNYTSGTTADPKGVVIShRGAYLSTLSAIIG--WEMGTCPVYLWTLPMfhcnGWT-----FTWG 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 318 PLANGTTTLMFEGVpqypTWARLGHVVDKHKVSILYTAPTAIRAMMrEGDSYVRESNRSSLRLLGSVGEPinPEAWNWYY 397
Cdd:PLN03102 248 TAARGGTSVCMRHV----TAPEIYKNIEMHNVTHMCCVPTVFNILL-KGNSLDLSPRSGPVHVLTGGSPP--PAALVKKV 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 398 NVVG----------EGRCPIVDTWWQTETGGIliaPLPGATALKPGSATRPLFGIQPAIVDGEGNE---LEGAAEGNLVI 464
Cdd:PLN03102 321 QRLGfqvmhaygltEATGPVLFCEWQDEWNRL---PENQQMELKARQGVSILGLADVDVKNKETQEsvpRDGKTMGEIVI 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 465 KDSwpGQMRTIWGDPdrfiEAYFSTFKNTYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEA 543
Cdd:PLN03102 398 KGS--SIMKGYLKNP----KATSEAFKHGWLnTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLET 471
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 544 AVVGMPHDIKGQGICTFVTLQAGVPESEEL-------RKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKI 616
Cdd:PLN03102 472 AVVAMPHPTWGETPCAFVVLEKGETTKEDRvdklvtrERDLIEYCRENLPHFMCPRKVVFLQELPKNGNGKILKPKLRDI 551
|
.
gi 446925704 617 A 617
Cdd:PLN03102 552 A 552
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
80-613 |
2.17e-23 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 106.41 E-value: 2.17e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCV 159
Cdd:PRK05691 1137 LNEQARQTPERIALVWDGGS------LDYAELHAQANRLAHYLRDKGVGPDVCVAIAAERSPQLLVGLLAILKAGGAYVP 1210
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFGGFSPDSLASRIEDSQAKLVITADSSLRAgkllplkenvdlalaLPGTECVenvivvyrnaNPIEMKPgrdlwYHLii 239
Cdd:PRK05691 1211 LDPDYPAERLAYMLADSGVELLLTQSHLLER---------------LPQAEGV----------SAIALDS-----LHL-- 1258
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 240 mevdANCPPEP----MKAEDPLFILYTSGSTGKPKGVLHTTGGY---LVYVASTFKevfdLKQDDVYWCTADVG-----W 307
Cdd:PRK05691 1259 ----DSWPSQApglhLHGDNLAYVIYTSGSTGQPKGVGNTHAALaerLQWMQATYA----LDDSDVLMQKAPISfdvsvW 1330
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 308 ------ITGHSYLIYGPlangtttlmfeGVPQYPtwARLGHVVDKHKVSILYTAPTAIRAMMREGDSyvreSNRSSLRLL 381
Cdd:PRK05691 1331 ecfwplITGCRLVLAGP-----------GEHRDP--QRIAELVQQYGVTTLHFVPPLLQLFIDEPLA----AACTSLRRL 1393
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 382 GSVGEPINPEAWN---------WYYNVVGEGRCPIVDTWWQTETGGILIAPLpgatalkpgsaTRPLFGIQPAIVDGEGN 452
Cdd:PRK05691 1394 FSGGEALPAELRNrvlqrlpqvQLHNRYGPTETAINVTHWQCQAEDGERSPI-----------GRPLGNVLCRVLDAELN 1462
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 453 ELEGAAEGNLVIKDSwpGQMRTIWGDP----DRFI-EAYFSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGT 527
Cdd:PRK05691 1463 LLPPGVAGELCIGGA--GLARGYLGRPaltaERFVpDPLGEDGARLYRTGDRARWNADGALEYLGRLDQQVKLRGFRVEP 1540
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 528 AEIESALVSHEAVAEAAVVgMPHDIKGQGICTFVTLQAGvPESEELRkeLISWVRKVLGPVASPDALHWAPALPKTRSGK 607
Cdd:PRK05691 1541 EEIQARLLAQPGVAQAAVL-VREGAAGAQLVGYYTGEAG-QEAEAER--LKAALAAELPEYMVPAQLIRLDQMPLGPSGK 1616
|
....*.
gi 446925704 608 IMRRIL 613
Cdd:PRK05691 1617 LDRRAL 1622
|
|
| A_NRPS_GliP_like |
cd17653 |
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ... |
81-615 |
3.41e-23 |
|
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341308 [Multi-domain] Cd Length: 433 Bit Score: 102.77 E-value: 3.41e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 81 DRHLKEHPHKPAIiwegDHPSRHkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVV 160
Cdd:cd17653 4 ERIAAAHPDAVAV----ESLGGS--LTYGELDAASNALANRLLQLGVVPGDVVPLLSDRSLEMLVAILAILKAGAAYVPL 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 161 FGGFSPDSLASRIEDSQAKLVITADSslragkllplkenvdlalalpgtecvenvivvyrnanpiemkpgrdlwyhliim 240
Cdd:cd17653 78 DAKLPSARIQAILRTSGATLLLTTDS------------------------------------------------------ 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 241 evdancppepmkAEDPLFILYTSGSTGKPKGVLHTTGGYLVYV-----------ASTFKEVFDLKqddvYWCTADVgwit 309
Cdd:cd17653 104 ------------PDDLAYIIFTSGSTGIPKGVMVPHRGVLNYVsqpparldvgpGSRVAQVLSIA----FDACIGE---- 163
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 310 ghsylIYGPLANGTTtLMFEGVPQypTWArlgHVVDKhkVSILYTAPTAIrammregdSYVRESNRSSLRLLGSVGEPIN 389
Cdd:cd17653 164 -----IFSTLCNGGT-LVLADPSD--PFA---HVART--VDALMSTPSIL--------STLSPQDFPNLKTIFLGGEAVP 222
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 390 P---EAWnwyynvvGEGRCpIVDTWWQTETGgILIAplpgATALKPGSAT---RPLFGIQPAIVDGEGNELEGAAEGNLV 463
Cdd:cd17653 223 PsllDRW-------SPGRR-LYNAYGPTECT-ISST----MTELLPGQPVtigKPIPNSTCYILDADLQPVPEGVVGEIC 289
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 464 IkdSWPGQMRTIWGDP----DRFIEAYFSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEA 539
Cdd:cd17653 290 I--SGVQVARGYLGNPaltaSKFVPDPFWPGSRMYRTGDYGRWTEDGGLEFLGREDNQVKVRGFRINLEEIEEVVLQSQP 367
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 446925704 540 VAEAAVVGMPHDIkgqgICTFVTlqagvPES---EELRKELiswvRKVLGPVASPDALHWAPALPKTRSGKIMRRILRK 615
Cdd:cd17653 368 EVTQAAAIVVNGR----LVAFVT-----PETvdvDGLRSEL----AKHLPSYAVPDRIIALDSFPLTANGKVDRKALRE 433
|
|
| PRK07638 |
PRK07638 |
acyl-CoA synthetase; Validated |
88-621 |
8.67e-23 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236071 [Multi-domain] Cd Length: 487 Bit Score: 102.16 E-value: 8.67e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 88 PHKPAIIwEGDhpsrhKIVSYKELHDEVCRFANVLKKYGiGKGDRVVLYMPMVTE-----AAIAMLACArigavhCVVFG 162
Cdd:PRK07638 15 PNKIAIK-END-----RVLTYKDWFESVCKVANWLNEKE-SKNKTIAILLENRIEflqlfAGAAMAGWT------CVPLD 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 163 -GFSPDSLASRIEDSQAKLVITADSSLRAgkllplkenvdlalaLPGTECveNVIVVyrnanpiemkpgrDLWYHLIIME 241
Cdd:PRK07638 82 iKWKQDELKERLAISNADMIVTERYKLND---------------LPDEEG--RVIEI-------------DEWKRMIEKY 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 242 VDANCPPEPMKaEDPLFILYTSGSTGKPKGVLHTTGGYLvyvastfkEVFDLKQDDVYWCTADVGWITG---HSYLIYGP 318
Cdd:PRK07638 132 LPTYAPIENVQ-NAPFYMGFTSGSTGKPKAFLRAQQSWL--------HSFDCNVHDFHMKREDSVLIAGtlvHSLFLYGA 202
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 319 LA----NGTTTLMFEGVP-QYPTWARLGhvvdkhKVSILYTAPTAIRAMMREgdSYVRESnrsSLRLLGSVGE------- 386
Cdd:PRK07638 203 IStlyvGQTVHLMRKFIPnQVLDKLETE------NISVMYTVPTMLESLYKE--NRVIEN---KMKIISSGAKweaeake 271
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 387 ---PINPEAWnwYYNVVGEGRCPIVDtwwqtetggiliAPLPGATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGNLV 463
Cdd:PRK07638 272 kikNIFPYAK--LYEFYGASELSFVT------------ALVDEESERRPNSVGRPFHNVQVRICNEAGEEVQKGEIGTVY 337
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 464 IKDswpgqmrtiwgdPDRF----IEAYFSTFKNT--YFT-GDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVS 536
Cdd:PRK07638 338 VKS------------PQFFmgyiIGGVLARELNAdgWMTvRDVGYEDEEGFIYIVGREKNMILFGGINIFPEEIESVLHE 405
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 537 HEAVAEAAVVGMPHDIKGQGICTFVtlqagvpESEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKI 616
Cdd:PRK07638 406 HPAVDEIVVIGVPDSYWGEKPVAII-------KGSATKQQLKSFCLQRLSSFKIPKEWHFVDEIPYTNSGKIARMEAKSW 478
|
....*
gi 446925704 617 AANEL 621
Cdd:PRK07638 479 IENQE 483
|
|
| PRK05677 |
PRK05677 |
long-chain-fatty-acid--CoA ligase; Validated |
104-614 |
1.30e-22 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 168170 [Multi-domain] Cd Length: 562 Bit Score: 102.15 E-value: 1.30e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 104 KIVSYKELHDEVCRFANVLKKY-GIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAK-LV 181
Cdd:PRK05677 48 KTLTYGELYKLSGAFAAWLQQHtDLKPGDRIAVQLPNVLQYPVAVFGAMRAGLIVVNTNPLYTAREMEHQFNDSGAKaLV 127
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 182 ITADSSLRAGKLLP--------LKENVDLALALPGTecVENVIVVYRNanpiEMKPGrdlwYHL-------IIMEVDANC 246
Cdd:PRK05677 128 CLANMAHLAEKVLPktgvkhviVTEVADMLPPLKRL--LINAVVKHVK----KMVPA----YHLpqavkfnDALAKGAGQ 197
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 247 PPEPM--KAEDPLFILYTSGSTGKPKGVLHTtggYLVYVASTFKevfdlkqddvywCTAdvgwitghsyLIYGPLANGTT 324
Cdd:PRK05677 198 PVTEAnpQADDVAVLQYTGGTTGVAKGAMLT---HRNLVANMLQ------------CRA----------LMGSNLNEGCE 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 325 TLMfEGVPQYPTWARLGH-----VVDKHkvSILYTAPTAIRAMMRE--------------------GDSYVRESNRSSLR 379
Cdd:PRK05677 253 ILI-APLPLYHIYAFTFHcmammLIGNH--NILISNPRDLPAMVKElgkwkfsgfvglntlfvalcNNEAFRKLDFSALK 329
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 380 LLGSVGEPINPEAWNWYYNVVGegrCPIVDTWWQTETGGIliAPLPGATALKPGSATRPLFGIQPAIVDGEGNELEGAAE 459
Cdd:PRK05677 330 LTLSGGMALQLATAERWKEVTG---CAICEGYGMTETSPV--VSVNPSQAIQVGTIGIPVPSTLCKVIDDDGNELPLGEV 404
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 460 GNLVIKDswPGQMRTIWGDPDRFIEAYFST--FKntyfTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSH 537
Cdd:PRK05677 405 GELCVKG--PQVMKGYWQRPEATDEILDSDgwLK----TGDIALIQEDGYMRIVDRKKDMILVSGFNVYPNELEDVLAAL 478
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446925704 538 EAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEElrkELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILR 614
Cdd:PRK05677 479 PGVLQCAAIGVPDEKSGEAIKVFVVVKPGETLTKE---QVMEHMRANLTGYKVPKAVEFRDELPTTNVGKILRRELR 552
|
|
| ttLC_FACS_like |
cd05915 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
107-615 |
2.37e-22 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.
Pssm-ID: 213283 [Multi-domain] Cd Length: 509 Bit Score: 100.97 E-value: 2.37e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 107 SYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKlVITADS 186
Cdd:cd05915 26 TYAEVYQRARRLMGGLRALGVGVGDRVATLGFNHFRHLEAYFAVPGMGAVLHTANPRLSPKEIAYILNHAEDK-VLLFDP 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 187 SLragkllplkenvdLALALPGTECVENVivvyrNANPIEMKPGRDlwYHLIIMEVDANCPP-EPMKAEDPLFILYTSGS 265
Cdd:cd05915 105 NL-------------LPLVEAIRGELKTV-----QHFVVMDEKAPE--GYLAYEEALGEEADpVRVPERAACGMAYTTGT 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 266 TGKPKGVLHT-TGGYLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYlIYGPLANGTTTLMFEGVPQYPTwarLGHVV 344
Cdd:cd05915 165 TGLPKGVVYShRALVLHSLAASLVDGTALSEKDVVLPVVPMFHVNAWCL-PYAATLVGAKQVLPGPRLDPAS---LVELF 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 345 DKHKVSILYTAPTAIRAMMREGDSyVRESNRSSLRLLGSVGEPinPEAwnwYYNVVGEGRCPIVDTWWQTETGGILIA-- 422
Cdd:cd05915 241 DGEGVTFTAGVPTVWLALADYLES-TGHRLKTLRRLVVGGSAA--PRS---LIARFERMGVEVRQGYGLTETSPVVVQnf 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 423 ------PLPGATALKPgSATRPLFGIQPAIVDGEGNELEGAAEGNLVikdswpgqmRTIWGDPDRFIEAYFSTFKNT--- 493
Cdd:cd05915 315 vkshleSLSEEEKLTL-KAKTGLPIPLVRLRVADEEGRPVPKDGKAL---------GEVQLKGPWITGGYYGNEEATrsa 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 494 ------YFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQagv 567
Cdd:cd05915 385 ltpdgfFRTGDIAVWDEEGYVEIKDRLKDLIKSGGEWISSVDLENALMGHPKVKEAAVVAIPHPKWQERPLAVVVPR--- 461
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 446925704 568 pESEELRKELISWVRKVLGPVAS-PDALHWAPALPKTRSGKIMRRILRK 615
Cdd:cd05915 462 -GEKPTPEELNEHLLKAGFAKWQlPDAYVFAEEIPRTSAGKFLKRALRE 509
|
|
| PRK05852 |
PRK05852 |
fatty acid--CoA ligase family protein; |
77-611 |
3.90e-22 |
|
fatty acid--CoA ligase family protein;
Pssm-ID: 235625 [Multi-domain] Cd Length: 534 Bit Score: 100.35 E-value: 3.90e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 77 ANCLDRHLKEHPHKPAIIWEGDHPSrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAV 156
Cdd:PRK05852 19 ADLVEVAATRLPEAPALVVTADRIA----ISYRDLARLVDDLAGQLTRSGLLPGDRVALRMGSNAEFVVALLAASRADLV 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 157 HCVVFGGFSPDSLASRIEDSQAKLVIT-----ADSSLRAGKLLPLKENVDlalalPGTECVENVIVVYRNANpIEMKPgr 231
Cdd:PRK05852 95 VVPLDPALPIAEQRVRSQAAGARVVLIdadgpHDRAEPTTRWWPLTVNVG-----GDSGPSGGTLSVHLDAA-TEPTP-- 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 232 dlwyhliimevdANCPPEPMKAEDPLfILYTSGSTGKPKGVLHTTGGylvyVASTFKEV---FDLKQDDVywCTADVGWI 308
Cdd:PRK05852 167 ------------ATSTPEGLRPDDAM-IMFTGGTTGLPKMVPWTHAN----IASSVRAIitgYRLSPRDA--TVAVMPLY 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 309 TGHsyliyGPLANGTTTLMFEGVPQYPTWARL-GHVV--DKHKV-SILYTA-PTAIRAMMREGDSYVRESNRSSLRLLGS 383
Cdd:PRK05852 228 HGH-----GLIAALLATLASGGAVLLPARGRFsAHTFwdDIKAVgATWYTAvPTIHQILLERAATEPSGRKPAALRFIRS 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 384 VGEPINPEAWNWYYNVVGegrCPIVDTWWQTET---------GGILIAPLPGATALKPGSATrplfGIQPAIVDGEGNEL 454
Cdd:PRK05852 303 CSAPLTAETAQALQTEFA---APVVCAFGMTEAthqvtttqiEGIGQTENPVVSTGLVGRST----GAQIRIVGSDGLPL 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 455 EGAAEGNLVIKDswPGQMRTIWGDPDrfIEAyfSTFKNTYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESA 533
Cdd:PRK05852 376 PAGAVGEVWLRG--TTVVRGYLGDPT--ITA--ANFTDGWLrTGDLGSLSAAGDLSIRGRIKELINRGGEKISPERVEGV 449
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446925704 534 LVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESeelRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRR 611
Cdd:PRK05852 450 LASHPNVMEAAVFGVPDQLYGEAVAAVIVPRESAPPT---AEELVQFCRERLAAFEIPASFQEASGLPHTAKGSLDRR 524
|
|
| ACAS_N |
pfam16177 |
Acetyl-coenzyme A synthetase N-terminus; This domain is found at the N-terminus of many ... |
22-79 |
4.48e-22 |
|
Acetyl-coenzyme A synthetase N-terminus; This domain is found at the N-terminus of many acetyl-coenzyme A synthetase enzymes.
Pssm-ID: 465043 [Multi-domain] Cd Length: 55 Bit Score: 89.45 E-value: 4.48e-22
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|....*...
gi 446925704 22 YFKRYQHSIENPDEFWAEQAKIVDWIKPFTQVKNTSFDKDnfkIEWFADGELNVSANC 79
Cdd:pfam16177 1 YEALYRRSIEDPEGFWGEVAKELDWFKPFDKVLDGSNGPF---AKWFVGGKLNVCYNC 55
|
|
| PRK07514 |
PRK07514 |
malonyl-CoA synthase; Validated |
88-614 |
4.62e-22 |
|
malonyl-CoA synthase; Validated
Pssm-ID: 181011 [Multi-domain] Cd Length: 504 Bit Score: 99.95 E-value: 4.62e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 88 PHKPAIiwEGDHPSRhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPD 167
Cdd:PRK07514 16 RDAPFI--ETPDGLR---YTYGDLDAASARLANLLVALGVKPGDRVAVQVEKSPEALALYLATLRAGAVFLPLNTAYTLA 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 168 SLASRIEDSQAKLVITADSslRAGKLLPLKENVdlalalpGTECVENVivvyrnanpiemkpgrDLWYHLIIMEVDANCP 247
Cdd:PRK07514 91 ELDYFIGDAEPALVVCDPA--NFAWLSKIAAAA-------GAPHVETL----------------DADGTGSLLEAAAAAP 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 248 PE----PMKAEDPLFILYTSGSTGKPKGVLHTTGGyLVYVASTFKEVFDLKQDDVYWctadvgwitgHSYLIY------- 316
Cdd:PRK07514 146 DDfetvPRGADDLAAILYTSGTTGRSKGAMLSHGN-LLSNALTLVDYWRFTPDDVLI----------HALPIFhthglfv 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 317 ---GPLANG--------------------TTTLMfeGVPQYptwarlghvvdkhkvsilYTaptaiRamMREGDSYVRES 373
Cdd:PRK07514 215 atnVALLAGasmiflpkfdpdavlalmprATVMM--GVPTF------------------YT-----R--LLQEPRLTREA 267
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 374 NRSsLRLLGSVGEPINPEAWNWYYNVVGEgrcPIVDTWWQTETGGILIAPLPGATalKPGSATRPLFGIQPAIVDGE-GN 452
Cdd:PRK07514 268 AAH-MRLFISGSAPLLAETHREFQERTGH---AILERYGMTETNMNTSNPYDGER--RAGTVGFPLPGVSLRVTDPEtGA 341
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 453 ELEGAAEGNLVIKDswPGQMRTIWGDPDRFIEAYFStfkNTYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIE 531
Cdd:PRK07514 342 ELPPGEIGMIEVKG--PNVFKGYWRMPEKTAEEFRA---DGFFiTGDLGKIDERGYVHIVGRGKDLIISGGYNVYPKEVE 416
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 532 SALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEElrkELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRR 611
Cdd:PRK07514 417 GEIDELPGVVESAVIGVPHPDFGEGVTAVVVPKPGAALDEA---AILAALKGRLARFKQPKRVFFVDELPRNTMGKVQKN 493
|
...
gi 446925704 612 ILR 614
Cdd:PRK07514 494 LLR 496
|
|
| PRK12492 |
PRK12492 |
long-chain-fatty-acid--CoA ligase; Provisional |
81-617 |
1.11e-21 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 171539 [Multi-domain] Cd Length: 562 Bit Score: 99.13 E-value: 1.11e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 81 DRHLKEHPHKPAIIWEGdhpsrhKIVSYKELHDEVCRFANVLKKY-GIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCV 159
Cdd:PRK12492 31 ERSCKKFADRPAFSNLG------VTLSYAELERHSAAFAAYLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVN 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFGGFSPDSLASRIEDSQAK-LV-----------ITADSSL------RAGKLLPLKENvdlALALPGTECVENVIVVYRN 221
Cdd:PRK12492 105 TNPLYTAREMRHQFKDSGARaLVylnmfgklvqeVLPDTGIeylieaKMGDLLPAAKG---WLVNTVVDKVKKMVPAYHL 181
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 222 ANPIEMKpgrdlwyHLIIMEVDANCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYlvyvastfkeVFDLKQddVYWC 301
Cdd:PRK12492 182 PQAVPFK-------QALRQGRGLSLKPVPVGLDDIAVLQYTGGTTGLAKGAMLTHGNL----------VANMLQ--VRAC 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 302 TADVGwITGHsyliygPLANGTTTLMFEGVPQYPTWARLGH-----VVDKHKVsiLYTAPTAIRAMMREGDSY------- 369
Cdd:PRK12492 243 LSQLG-PDGQ------PLMKEGQEVMIAPLPLYHIYAFTANcmcmmVSGNHNV--LITNPRDIPGFIKELGKWrfsallg 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 370 -------------VRESNRSSLRLLGSVGEPI---NPEAWNwyyNVVGegrCPIVDTWWQTETGGILIAPlPGATALKPG 433
Cdd:PRK12492 314 lntlfvalmdhpgFKDLDFSALKLTNSGGTALvkaTAERWE---QLTG---CTIVEGYGLTETSPVASTN-PYGELARLG 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 434 SATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDswPGQMRTIWGDPDRFIEAYFST--FKntyfTGDGARRDEDGYYWIT 511
Cdd:PRK12492 387 TVGIPVPGTALKVIDDDGNELPLGERGELCIKG--PQVMKGYWQQPEATAEALDAEgwFK----TGDIAVIDPDGFVRIV 460
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 512 GRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEELRkeliSWVRKVLGPVASP 591
Cdd:PRK12492 461 DRKKDLIIVSGFNVYPNEIEDVVMAHPKVANCAAIGVPDERSGEAVKLFVVARDPGLSVEELK----AYCKENFTGYKVP 536
|
570 580
....*....|....*....|....*.
gi 446925704 592 DALHWAPALPKTRSGKIMRRILRKIA 617
Cdd:PRK12492 537 KHIVLRDSLPMTPVGKILRRELRDIA 562
|
|
| AFD_YhfT-like |
cd17633 |
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ... |
255-610 |
2.00e-21 |
|
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain
Pssm-ID: 341288 [Multi-domain] Cd Length: 320 Bit Score: 95.55 E-value: 2.00e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 255 DPLFILYTSGSTGKPKGVLHTTGGYLvyvastfkEVFDLKQDDVYWCTADVGWITG---HSYLIYGPLA----NGTTTLM 327
Cdd:cd17633 1 NPFYIGFTSGTTGLPKAYYRSERSWI--------ESFVCNEDLFNISGEDAILAPGplsHSLFLYGAISalylGGTFIGQ 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 328 FEGVPqyptwARLGHVVDKHKVSILYTAPTAIRAMMREGdsyvreSNRSSLRLLGSVGEPINPEAWNWYYNVVGEGRcpI 407
Cdd:cd17633 73 RKFNP-----KSWIRKINQYNATVIYLVPTMLQALARTL------EPESKIKSIFSSGQKLFESTKKKLKNIFPKAN--L 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 408 VDTWWQTETGgiLIAPLPGATALKPGSATRPLFGIQPAIVDGEGNELegaaeGNLVIKDswpgQMRTiwgdpDRFIEAYF 487
Cdd:cd17633 140 IEFYGTSELS--FITYNFNQESRPPNSVGRPFPNVEIEIRNADGGEI-----GKIFVKS----EMVF-----SGYVRGGF 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 488 STFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQgICTFVTLQAGV 567
Cdd:cd17633 204 SNPDGWMSVGDIGYVDEEGYLYLVGRESDMIIIGGINIFPTEIESVLKAIPGIEEAIVVGIPDARFGE-IAVALYSGDKL 282
|
330 340 350 360
....*....|....*....|....*....|....*....|...
gi 446925704 568 peseeLRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMR 610
Cdd:cd17633 283 -----TYKQLKRFLKQKLSRYEIPKKIIFVDSLPYTSSGKIAR 320
|
|
| PRK08315 |
PRK08315 |
AMP-binding domain protein; Validated |
80-621 |
3.15e-21 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236236 [Multi-domain] Cd Length: 559 Bit Score: 97.96 E-value: 3.15e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIWegdhPSRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCV 159
Cdd:PRK08315 22 LDRTAARYPDREALVY----RDQGLRWTYREFNEEVDALAKGLLALGIEKGDRVGIWAPNVPEWVLTQFATAKIGAILVT 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFGGFSPDSLASRIEDSQAKLVITA----DSSLRA--GKLLP-LKENVDLALALPGTECVENVIVVYRnanpiEMKPGRD 232
Cdd:PRK08315 98 INPAYRLSELEYALNQSGCKALIAAdgfkDSDYVAmlYELAPeLATCEPGQLQSARLPELRRVIFLGD-----EKHPGML 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 233 LWYHLIIMEVDAncPPEPMKA-------EDPLFILYTSGSTGKPKGVLHT-----TGGYLVYVASTFKEvfdlkQDDV-- 298
Cdd:PRK08315 173 NFDELLALGRAV--DDAELAArqatldpDDPINIQYTSGTTGFPKGATLThrnilNNGYFIGEAMKLTE-----EDRLci 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 299 ----YWCTADV----GWITGHSYLIY-GPLANGTTTLmfegvpqyptwarlgHVVDKHKVSILYTAPTAIRAMM--REGD 367
Cdd:PRK08315 246 pvplYHCFGMVlgnlACVTHGATMVYpGEGFDPLATL---------------AAVEEERCTALYGVPTMFIAELdhPDFA 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 368 SYvresNRSSLR---LLGSVgepinpeawnwyynvvgegrCPI------VDTWWQTEtggILIAPlpGATALKPGS---- 434
Cdd:PRK08315 311 RF----DLSSLRtgiMAGSP--------------------CPIevmkrvIDKMHMSE---VTIAY--GMTETSPVStqtr 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 435 ---------AT--RPLFGIQPAIVDGE-GNELEGAAEGNLVIKdswpGQ--MRTIWGDPDRFIEAYFStfKNTYFTGDGA 500
Cdd:PRK08315 362 tddplekrvTTvgRALPHLEVKIVDPEtGETVPRGEQGELCTR----GYsvMKGYWNDPEKTAEAIDA--DGWMHTGDLA 435
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 501 RRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEElrkELISW 580
Cdd:PRK08315 436 VMDEEGYVNIVGRIKDMIIRGGENIYPREIEEFLYTHPKIQDVQVVGVPDEKYGEEVCAWIILRPGATLTEE---DVRDF 512
|
570 580 590 600
....*....|....*....|....*....|....*....|....
gi 446925704 581 VRkvlGPVAS---PDALHWAPALPKTRSGKIMRRILRKIAANEL 621
Cdd:PRK08315 513 CR---GKIAHykiPRYIRFVDEFPMTVTGKIQKFKMREMMIEEL 553
|
|
| PRK06018 |
PRK06018 |
putative acyl-CoA synthetase; Provisional |
107-615 |
5.74e-21 |
|
putative acyl-CoA synthetase; Provisional
Pssm-ID: 235673 [Multi-domain] Cd Length: 542 Bit Score: 97.13 E-value: 5.74e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 107 SYKELHDEVCRFANVLKKYGIGKGDRVvlympmvteAAIAM-----LAC----ARIGAVHCVVFGGFSPDSLASRIEDSQ 177
Cdd:PRK06018 41 TYAQIHDRALKVSQALDRDGIKLGDRV---------ATIAWntwrhLEAwygiMGIGAICHTVNPRLFPEQIAWIINHAE 111
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 178 AKLVITaDSSLragklLPLKENVdlALALPGtecVENVIVVYRNANPIEMKPGRDLWYHLIIMEVDANCPPEPMKAEDPL 257
Cdd:PRK06018 112 DRVVIT-DLTF-----VPILEKI--ADKLPS---VERYVVLTDAAHMPQTTLKNAVAYEEWIAEADGDFAWKTFDENTAA 180
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 258 FILYTSGSTGKPKGVLHT-TGGYLVYVASTFKEVFDLKQDDVY-----------WCTADVGWITGHSYLIYGPLANGttt 325
Cdd:PRK06018 181 GMCYTSGTTGDPKGVLYShRSNVLHALMANNGDALGTSAADTMlpvvplfhansWGIAFSAPSMGTKLVMPGAKLDG--- 257
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 326 lmfegvpqyptwARLGHVVDKHKVSILYTAPTAIRAMMREgdsyvRESNRSSLRLLGSV--GEPINPEAWNWYYNVVGeg 403
Cdd:PRK06018 258 ------------ASVYELLDTEKVTFTAGVPTVWLMLLQY-----MEKEGLKLPHLKMVvcGGSAMPRSMIKAFEDMG-- 318
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 404 rCPIVDTWWQTETGGI-LIAPLPGATALKPGSAT--------RPLFGIQPAIVDGEGNEL--EGAAEGNLVIkdSWPGQM 472
Cdd:PRK06018 319 -VEVRHAWGMTEMSPLgTLAALKPPFSKLPGDARldvlqkqgYPPFGVEMKITDDAGKELpwDGKTFGRLKV--RGPAVA 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 473 RTIWGDPDRFIEAyfstfkNTYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHD 551
Cdd:PRK06018 396 AAYYRVDGEILDD------DGFFdTGDVATIDAYGYMRITDRSKDVIKSGGEWISSIDLENLAVGHPKVAEAAVIGVYHP 469
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446925704 552 IKGQGICTFVTLQAGVPESeelRKELISWVRkvlGPVAS---PDALHWAPALPKTRSGKIMRRILRK 615
Cdd:PRK06018 470 KWDERPLLIVQLKPGETAT---REEILKYMD---GKIAKwwmPDDVAFVDAIPHTATGKILKTALRE 530
|
|
| A_NRPS_LgrA-like |
cd17645 |
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ... |
81-613 |
8.24e-21 |
|
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.
Pssm-ID: 341300 [Multi-domain] Cd Length: 440 Bit Score: 95.70 E-value: 8.24e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 81 DRHLKEHPHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVV 160
Cdd:cd17645 5 EEQVERTPDHVAVVDRGQS------LTYKQLNEKANQLARHLRGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVPI 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 161 FGGFSPDSLASRIEDSQAKLVITadsslragkllplkenvdlalalpgtecvenvivvyrnanpiemkpgrdlwyhliim 240
Cdd:cd17645 79 DPDYPGERIAYMLADSSAKILLT--------------------------------------------------------- 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 241 evdancppepmKAEDPLFILYTSGSTGKPKGVL--HTTggyLVYVASTFKEVFDLKQDDVYWCTADVGWiTGHSYLIYGP 318
Cdd:cd17645 102 -----------NPDDLAYVIYTSGSTGLPKGVMieHHN---LVNLCEWHRPYFGVTPADKSLVYASFSF-DASAWEIFPH 166
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 319 LANGTTTLMFEGVPQYPTwARLGHVVDKHKVSILYTaPTAIRAMMREGDSyvresnrSSLRLLGSVGEPINPEAWNWY-- 396
Cdd:cd17645 167 LTAGAALHVVPSERRLDL-DALNDYFNQEGITISFL-PTGAAEQFMQLDN-------QSLRVLLTGGDKLKKIERKGYkl 237
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 397 YNVVGEGRCPIVDTWWQT--ETGGILIAplpgatalKPGSATRPLfgiqpaiVDGEGNEL--EGAAeGNLVIKDSwpGQM 472
Cdd:cd17645 238 VNNYGPTENTVVATSFEIdkPYANIPIG--------KPIDNTRVY-------ILDEALQLqpIGVA-GELCIAGE--GLA 299
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 473 RTIWGDPD----RFIEAYFSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGM 548
Cdd:cd17645 300 RGYLNRPEltaeKFIVHPFVPGERMYRTGDLAKFLPDGNIEFLGRLDQQVKIRGYRIEPGEIEPFLMNHPLIELAAVLAK 379
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 446925704 549 PHDIKGQGICTFVTLQAGVPEsEELRkeliSWVRKVLGPVASPDALHWAPALPKTRSGKIMRRIL 613
Cdd:cd17645 380 EDADGRKYLVAYVTAPEEIPH-EELR----EWLKNDLPDYMIPTYFVHLKALPLTANGKVDRKAL 439
|
|
| PRK07059 |
PRK07059 |
Long-chain-fatty-acid--CoA ligase; Validated |
80-614 |
2.42e-20 |
|
Long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235923 [Multi-domain] Cd Length: 557 Bit Score: 95.09 E-value: 2.42e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIWEGdhpsrhKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCV 159
Cdd:PRK07059 29 LEESFRQYADRPAFICMG------KAITYGELDELSRALAAWLQSRGLAKGARVAIMMPNVLQYPVAIAAVLRAGYVVVN 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFGGFSPDSLASRIEDSQAKLVItadsslragkllpLKEN--VDLALALPGTEcVENVIV----------------VYRN 221
Cdd:PRK07059 103 VNPLYTPRELEHQLKDSGAEAIV-------------VLENfaTTVQQVLAKTA-VKHVVVasmgdllgfkghivnfVVRR 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 222 ANpiEMKPGRDLWYHLIIMEVDA-----NCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVAST---FKEVFDL 293
Cdd:PRK07059 169 VK--KMVPAWSLPGHVRFNDALAegarqTFKPVKLGPDDVAFLQYTGGTTGVSKGATLLHRNIVANVLQMeawLQPAFEK 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 294 KQDD---VYWCTADVgwitghsYLIYGPLANGTTTLMFEGV----PQYPTWARLGHVVDKHKVSILYTAPTAIRAMMREG 366
Cdd:PRK07059 247 KPRPdqlNFVCALPL-------YHIFALTVCGLLGMRTGGRniliPNPRDIPGFIKELKKYQVHIFPAVNTLYNALLNNP 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 367 DsyVRESNRSSLRL-LG---SVGEPInPEAWnwyYNVVGegrCPIVDTWWQTETGGILIAPLPGATALKpGSATRPLFGI 442
Cdd:PRK07059 320 D--FDKLDFSKLIVaNGggmAVQRPV-AERW---LEMTG---CPITEGYGLSETSPVATCNPVDATEFS-GTIGLPLPST 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 443 QPAIVDGEGNELEGAAEGNLVIKDswPGQMRTIWGDPDrfiEAYFSTFKNTYF-TGDGARRDEDGYYWITGRVDDVLNVS 521
Cdd:PRK07059 390 EVSIRDDDGNDLPLGEPGEICIRG--PQVMAGYWNRPD---ETAKVMTADGFFrTGDVGVMDERGYTKIVDRKKDMILVS 464
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 522 GHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVtlqagVPESEEL-RKELISWVRKVLGPVASPDALHWAPAL 600
Cdd:PRK07059 465 GFNVYPNEIEEVVASHPGVLEVAAVGVPDEHSGEAVKLFV-----VKKDPALtEEDVKAFCKERLTNYKRPKFVEFRTEL 539
|
570
....*....|....
gi 446925704 601 PKTRSGKIMRRILR 614
Cdd:PRK07059 540 PKTNVGKILRRELR 553
|
|
| FadD3 |
cd17638 |
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ... |
259-610 |
3.19e-20 |
|
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.
Pssm-ID: 341293 [Multi-domain] Cd Length: 330 Bit Score: 92.18 E-value: 3.19e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 259 ILYTSGSTGKPKGVL--HTTGgylVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTlmfegVPQ--Y 334
Cdd:cd17638 5 IMFTSGTTGRSKGVMcaHRQT---LRAAAAWADCADLTEDDRYLIINPFFHTFGYKAGIVACLLTGATV-----VPVavF 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 335 PTWARLgHVVDKHKVSILYTAPTAIRAMMREGDSyvRESNRSSLRLlgsvgepinpeawnwyyNVVGEGRCPIVDT-WWQ 413
Cdd:cd17638 77 DVDAIL-EAIERERITVLPGPPTLFQSLLDHPGR--KKFDLSSLRA-----------------AVTGAATVPVELVrRMR 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 414 TETGGILIAPLPGATALKPGSATRPlfGIQPAIVDG------EGNELEGAAEGNLVIKDswPGQMRTIWGDPDRFIEAYF 487
Cdd:cd17638 137 SELGFETVLTAYGLTEAGVATMCRP--GDDAETVATtcgracPGFEVRIADDGEVLVRG--YNVMQGYLDDPEATAEAID 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 488 StfKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGV 567
Cdd:cd17638 213 A--DGWLHTGDVGELDERGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVPDERMGEVGKAFVVARPGV 290
|
330 340 350 360
....*....|....*....|....*....|....*....|...
gi 446925704 568 PESEElrkELISWVRKVLGPVASPDALHWAPALPKTRSGKIMR 610
Cdd:cd17638 291 TLTEE---DVIAWCRERLANYKVPRFVRFLDELPRNASGKVMK 330
|
|
| PRK08633 |
PRK08633 |
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated |
82-621 |
5.98e-20 |
|
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
Pssm-ID: 236315 [Multi-domain] Cd Length: 1146 Bit Score: 94.99 E-value: 5.98e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 82 RHLKEHPHKPAIIwegDhpSRHKIVSYKELHDEVCRFANVLKKyGIGKGDRVVLYMPMVTEAAIAMLACARIGAVhcVVF 161
Cdd:PRK08633 623 DTAKRNWSRLAVA---D--STGGELSYGKALTGALALARLLKR-ELKDEENVGILLPPSVAGALANLALLLAGKV--PVN 694
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 162 GGF--SPDSLASRIEDSQAKLVITADSSLR----AGKLLPLKENV------DLALALPGTECVENVIVVYrnanpieMKP 229
Cdd:PRK08633 695 LNYtaSEAALKSAIEQAQIKTVITSRKFLEklknKGFDLELPENVkviyleDLKAKISKVDKLTALLAAR-------LLP 767
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 230 GRDLwyhliimevdANCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVAStFKEVFDLKQDDVywctadvgwIT 309
Cdd:PRK08633 768 ARLL----------KRLYGPTFKPDDTATIIFSSGSEGEPKGVMLSHHNILSNIEQ-ISDVFNLRNDDV---------IL 827
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 310 G-----HSYLIYGplangtTTLM--FEGVPQY----PTWAR-LGHVVDKHKVSILYTAPTAIRAMMRegdsyvreSNR-- 375
Cdd:PRK08633 828 SslpffHSFGLTV------TLWLplLEGIKVVyhpdPTDALgIAKLVAKHRATILLGTPTFLRLYLR--------NKKlh 893
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 376 ----SSLRLLGSVGEPINPEAWNWYYNVVGEgrcPIVDTWWQTETGGILIAPLPGA--------TALKPGSATRPLFGIQ 443
Cdd:PRK08633 894 plmfASLRLVVAGAEKLKPEVADAFEEKFGI---RILEGYGATETSPVASVNLPDVlaadfkrqTGSKEGSVGMPLPGVA 970
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 444 PAIVDGE-GNELEGAAEGNLVIKDswPGQMRTIWGDPDRFIEAYFSTFKNTYF-TGDGARRDEDGYYWITGRVDdvlnvs 521
Cdd:PRK08633 971 VRIVDPEtFEELPPGEDGLILIGG--PQVMKGYLGDPEKTAEVIKDIDGIGWYvTGDKGHLDEDGFLTITDRYS------ 1042
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 522 ghRLgtAEIESALVSHEAVAEA------------AVVGMPHDIKGQGICTFVTLQAGvpESEELRKELISwvrKVLGPVA 589
Cdd:PRK08633 1043 --RF--AKIGGEMVPLGAVEEElakalggeevvfAVTAVPDEKKGEKLVVLHTCGAE--DVEELKRAIKE---SGLPNLW 1113
|
570 580 590
....*....|....*....|....*....|..
gi 446925704 590 SPDALHWAPALPKTRSGKIMRRILRKIAANEL 621
Cdd:PRK08633 1114 KPSRYFKVEALPLLGSGKLDLKGLKELALALL 1145
|
|
| entE |
PRK10946 |
(2,3-dihydroxybenzoyl)adenylate synthase; |
80-621 |
6.68e-20 |
|
(2,3-dihydroxybenzoyl)adenylate synthase;
Pssm-ID: 236803 [Multi-domain] Cd Length: 536 Bit Score: 93.52 E-value: 6.68e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKehPHKPAIIwegdhpSRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVhcV 159
Cdd:PRK10946 31 LTRHAA--SDAIAVI------CGERQFSYRELNQASDNLACSLRRQGIKPGDTALVQLGNVAEFYITFFALLKLGVA--P 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFGGFSPDS--LASRIEDSQAKLVItADSSLragkllPLKENVDLALALpGTECVENVIVVYRNANPIEmkpGRDLWyhl 237
Cdd:PRK10946 101 VNALFSHQRseLNAYASQIEPALLI-ADRQH------ALFSDDDFLNTL-VAEHSSLRVVLLLNDDGEH---SLDDA--- 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 238 iIMEVDANCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYV-ASTfkEVFDLKQDDVYWCTADvgwiTGHSYLIY 316
Cdd:PRK10946 167 -INHPAEDFTATPSPADEVAFFQLSGGSTGTPKLIPRTHNDYYYSVrRSV--EICGFTPQTRYLCALP----AAHNYPMS 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 317 GP------LANGTTTLMFEgvpqyPTWARLGHVVDKHKVSILYTAPTAIRAMMREGDSYVRESNRSSLRLL--------G 382
Cdd:PRK10946 240 SPgalgvfLAGGTVVLAPD-----PSATLCFPLIEKHQVNVTALVPPAVSLWLQAIAEGGSRAQLASLKLLqvggarlsE 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 383 SVGEPINPEAWNWYYNVVG--EGrcpIV------DTWWQTETggiliaplpgatalkpgSATRPlfgIQPA----IVDGE 450
Cdd:PRK10946 315 TLARRIPAELGCQLQQVFGmaEG---LVnytrldDSDERIFT-----------------TQGRP---MSPDdevwVADAD 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 451 GNELEGAAEGNLVIKDswPGQMRTIWGDPDRFIEAYFStfKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEI 530
Cdd:PRK10946 372 GNPLPQGEVGRLMTRG--PYTFRGYYKSPQHNASAFDA--NGFYCSGDLVSIDPDGYITVVGREKDQINRGGEKIAAEEI 447
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 531 ESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVpESEELRKELISwvrkvLGpVAS---PDALHWAPALPKTRSGK 607
Cdd:PRK10946 448 ENLLLRHPAVIHAALVSMEDELMGEKSCAFLVVKEPL-KAVQLRRFLRE-----QG-IAEfklPDRVECVDSLPLTAVGK 520
|
570
....*....|....
gi 446925704 608 IMRRILRKIAANEL 621
Cdd:PRK10946 521 VDKKQLRQWLASRA 534
|
|
| PRK07008 |
PRK07008 |
long-chain-fatty-acid--CoA ligase; Validated |
261-614 |
2.82e-19 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235908 [Multi-domain] Cd Length: 539 Bit Score: 91.69 E-value: 2.82e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 261 YTSGSTGKPKGVLHTTGGYLVYV-ASTFKEVFDLKQDDVY-----------WCTADVGWITGHSYLIYGPLANGtttlmf 328
Cdd:PRK07008 183 YTSGTTGNPKGALYSHRSTVLHAyGAALPDAMGLSARDAVlpvvpmfhvnaWGLPYSAPLTGAKLVLPGPDLDG------ 256
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 329 egvpqyptwARLGHVVDKHKVSILYTAPTAIRAMMregdSYVRESNR--SSLR---LLGSVGEPINPEAWNWYYNVvgeg 403
Cdd:PRK07008 257 ---------KSLYELIEAERVTFSAGVPTVWLGLL----NHMREAGLrfSTLRrtvIGGSACPPAMIRTFEDEYGV---- 319
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 404 rcPIVDTWWQTEtggilIAPLPGATALK------PGSAT--------RPLFGIQPAIVDGEGNEL--EGAAEGNLVIKDS 467
Cdd:PRK07008 320 --EVIHAWGMTE-----MSPLGTLCKLKwkhsqlPLDEQrkllekqgRVIYGVDMKIVGDDGRELpwDGKAFGDLQVRGP 392
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 468 WpgqmrtiwgdpdrFIEAYF----STFKNTYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAE 542
Cdd:PRK07008 393 W-------------VIDRYFrgdaSPLVDGWFpTGDVATIDADGFMQITDRSKDVIKSGGEWISSIDIENVAVAHPAVAE 459
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 446925704 543 AAVVGMPHDIKGQGICTFVTLQAGVpesEELRKELISWVRkvlGPVAS---PDALHWAPALPKTRSGKIMRRILR 614
Cdd:PRK07008 460 AACIACAHPKWDERPLLVVVKRPGA---EVTREELLAFYE---GKVAKwwiPDDVVFVDAIPHTATGKLQKLKLR 528
|
|
| PRK05620 |
PRK05620 |
long-chain fatty-acid--CoA ligase; |
95-619 |
5.43e-19 |
|
long-chain fatty-acid--CoA ligase;
Pssm-ID: 180167 [Multi-domain] Cd Length: 576 Bit Score: 91.00 E-value: 5.43e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 95 WEGDHPSRhkiVSYKELHDEVCRFANVLK-KYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVhcvvfggFSP------- 166
Cdd:PRK05620 31 WGGAEQEQ---TTFAAIGARAAALAHALHdELGITGDQRVGSMMYNCAEHLEVLFAVACMGAV-------FNPlnkqlmn 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 167 DSLASRIEDSQAKlVITADSSLrAGKLLPLkenvdlalaLPGTECVENVIVVYRN---ANPIEMKPGRDLWYHLIIMEVD 243
Cdd:PRK05620 101 DQIVHIINHAEDE-VIVADPRL-AEQLGEI---------LKECPCVRAVVFIGPSdadSAAAHMPEGIKVYSYEALLDGR 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 244 ANCPPEPMKAE-DPLFILYTSGSTGKPKGVLHTTggylvyvASTFKEVFDLKqddvywcTADVGWIT-GHSYL----IYG 317
Cdd:PRK05620 170 STVYDWPELDEtTAAAICYSTGTTGAPKGVVYSH-------RSLYLQSLSLR-------TTDSLAVThGESFLccvpIYH 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 318 PLANGT--------TTLMFEGVPQYPtwARLGHVVDKHKVSILYTAPTAIRAMMREgdsYVRES-NRSSLRLLGSVGEPI 388
Cdd:PRK05620 236 VLSWGVplaafmsgTPLVFPGPDLSA--PTLAKIIATAMPRVAHGVPTLWIQLMVH---YLKNPpERMSLQEIYVGGSAV 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 389 NP---EAWNWYYNVvgegrcPIVDTWWQTETGGI-LIAPLPGATALKPGSATRPLFGIQPA-----IVDgEGNELEGA-- 457
Cdd:PRK05620 311 PPiliKAWEERYGV------DVVHVWGMTETSPVgTVARPPSGVSGEARWAYRVSQGRFPAsleyrIVN-DGQVMESTdr 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 458 AEGNLVIKDSWPGQmrTIWGDPDRFIEAYFSTFK-------NTYFTGDGARR-------DEDGYYWITGRVDDVLNVSGH 523
Cdd:PRK05620 384 NEGEIQVRGNWVTA--SYYHSPTEEGGGAASTFRgedvedaNDRFTADGWLRtgdvgsvTRDGFLTIHDRARDVIRSGGE 461
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 524 RLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEELRKELISWVRKVLGPVASPDALHWAPALPKT 603
Cdd:PRK05620 462 WIYSAQLENYIMAAPEVVECAVIGYPDDKWGERPLAVTVLAPGIEPTRETAERLRDQLRDRLPNWMLPEYWTFVDEIDKT 541
|
570
....*....|....*.
gi 446925704 604 RSGKIMRRILRKIAAN 619
Cdd:PRK05620 542 SVGKFDKKDLRQHLAD 557
|
|
| PLN02479 |
PLN02479 |
acetate-CoA ligase |
80-617 |
1.18e-18 |
|
acetate-CoA ligase
Pssm-ID: 178097 [Multi-domain] Cd Length: 567 Bit Score: 89.90 E-value: 1.18e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIwegdHPSRHkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCV 159
Cdd:PLN02479 26 LERAAVVHPTRKSVV----HGSVR--YTWAQTYQRCRRLASALAKRSIGPGSTVAVIAPNIPAMYEAHFGVPMAGAVVNC 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFGGFSPDSLASRIEDSQAKLVITADsslragKLLPLKENvdlALALpgtecvenvivvyrnanpIEMKPGRDLWYHLII 239
Cdd:PLN02479 100 VNIRLNAPTIAFLLEHSKSEVVMVDQ------EFFTLAEE---ALKI------------------LAEKKKSSFKPPLLI 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 240 MEVDANCPPEPMKAE---------------DPLF-------------ILYTSGSTGKPKGV-LHTTGGYLVYVASTFkeV 290
Cdd:PLN02479 153 VIGDPTCDPKSLQYAlgkgaieyekfletgDPEFawkppadewqsiaLGYTSGTTASPKGVvLHHRGAYLMALSNAL--I 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 291 FDLKQDDVY-----------WC-TADVGWITGHSYL--------IYGPLAN-GTTTL--------MFEGVPQYPTWARLG 341
Cdd:PLN02479 231 WGMNEGAVYlwtlpmfhcngWCfTWTLAALCGTNIClrqvtakaIYSAIANyGVTHFcaapvvlnTIVNAPKSETILPLP 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 342 HVVDkhkVSILYTAPTA--IRAMMREGdsyvresnrssLRLLGSVG--EPINPE---AWNWYYNvvgegRCPIVDTWWQT 414
Cdd:PLN02479 311 RVVH---VMTAGAAPPPsvLFAMSEKG-----------FRVTHTYGlsETYGPStvcAWKPEWD-----SLPPEEQARLN 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 415 ETGGILIAPLPGATALKPGSatrplfgIQPAIVDGEgNELEGAAEGNLVIKdswpGQMRtiwgDPdrfiEAYFSTFKNTY 494
Cdd:PLN02479 372 ARQGVRYIGLEGLDVVDTKT-------MKPVPADGK-TMGEIVMRGNMVMK----GYLK----NP----KANEEAFANGW 431
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 495 F-TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEE- 572
Cdd:PLN02479 432 FhSGDLGVKHPDGYIEIKDRSKDIIISGGENISSLEVENVVYTHPAVLEASVVARPDERWGESPCAFVTLKPGVDKSDEa 511
|
570 580 590 600
....*....|....*....|....*....|....*....|....*.
gi 446925704 573 -LRKELISWVRKVLGPVASPDALHWAPaLPKTRSGKIMRRILRKIA 617
Cdd:PLN02479 512 aLAEDIMKFCRERLPAYWVPKSVVFGP-LPKTATGKIQKHVLRAKA 556
|
|
| PRK08751 |
PRK08751 |
long-chain fatty acid--CoA ligase; |
77-618 |
1.21e-18 |
|
long-chain fatty acid--CoA ligase;
Pssm-ID: 181546 [Multi-domain] Cd Length: 560 Bit Score: 89.94 E-value: 1.21e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 77 ANCLDRHLKEHPHKPAiiwegdHPSRHKIVSYKELHDEVCRFAN-VLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGA 155
Cdd:PRK08751 28 AEVFATSVAKFADRPA------YHSFGKTITYREADQLVEQFAAyLLGELQLKKGDRVALMMPNCLQYPIATFGVLRAGL 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 156 VHCVVFGGFSPDSLASRIEDSQAKLVITADSSLRAGKLL----PLKENV-----DLaLALPGTECVENVIVVYRNANPIE 226
Cdd:PRK08751 102 TVVNVNPLYTPRELKHQLIDSGASVLVVIDNFGTTVQQViadtPVKQVIttglgDM-LGFPKAALVNFVVKYVKKLVPEY 180
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 227 MKPGRDLWYHLIIMEVDANCPPEPMKAEDPLFILYTSGSTGKPKGVLHTtggYLVYVASTfkevfdlkQDDVYWCTADVG 306
Cdd:PRK08751 181 RINGAIRFREALALGRKHSMPTLQIEPDDIAFLQYTGGTTGVAKGAMLT---HRNLVANM--------QQAHQWLAGTGK 249
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 307 WITGHS--------YLIYGPLANGTTTLMFEGVPQYPTWAR--LGHVVDKHKVSilYTAPTAIRAMMRE--GDSYVRESN 374
Cdd:PRK08751 250 LEEGCEvvitalplYHIFALTANGLVFMKIGGCNHLISNPRdmPGFVKELKKTR--FTAFTGVNTLFNGllNTPGFDQID 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 375 RSSLRLLGSVGEPINPEAWNWYYNVVGegrCPIVDTWWQTETG-GILIAPLpgatALKP--GSATRPLFGIQPAIVDGEG 451
Cdd:PRK08751 328 FSSLKMTLGGGMAVQRSVAERWKQVTG---LTLVEAYGLTETSpAACINPL----TLKEynGSIGLPIPSTDACIKDDAG 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 452 NELEGAAEGNLVIKDswPGQMRTIWGDPDRFIEAYFStfKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIE 531
Cdd:PRK08751 401 TVLAIGEIGELCIKG--PQVMKGYWKRPEETAKVMDA--DGWLHTGDIARMDEQGFVYIVDRKKDMILVSGFNVYPNEIE 476
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 532 SALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEELRKEliswVRKVLGPVASPDALHWAPALPKTRSGKIMRR 611
Cdd:PRK08751 477 DVIAMMPGVLEVAAVGVPDEKSGEIVKVVIVKKDPALTAEDVKAH----ARANLTGYKQPRIIEFRKELPKTNVGKILRR 552
|
....*..
gi 446925704 612 ILRKIAA 618
Cdd:PRK08751 553 ELRDAAK 559
|
|
| LC_FACL_like |
cd05914 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ... |
106-610 |
1.51e-18 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341240 [Multi-domain] Cd Length: 463 Bit Score: 89.04 E-value: 1.51e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 106 VSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITAD 185
Cdd:cd05914 8 LTYKDLADNIAKFALLLKINGVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSEAKAIFVSD 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 186 sslragkllplkeNVDLALalpgtecvenvivvyrnanpiemkpgrdlwyhliimevdancppepmkaedplfILYTSGS 265
Cdd:cd05914 88 -------------EDDVAL------------------------------------------------------INYTSGT 100
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 266 TGKPKGVLhTTGGYLVYVASTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFEGVP--------QYPTW 337
Cdd:cd05914 101 TGNSKGVM-LTYRNIVSNVDGVKEVVLLGKGDKILSILPLHHIYPLTFTLLLPLLNGAHVVFLDKIPsakiialaFAQVT 179
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 338 ARLG-----HVVDKHKVSI------------LYTAP--TAIRAMMRegdSYVRESNRSSLRLLGSVGEPINPEAWNWYYN 398
Cdd:cd05914 180 PTLGvpvplVIEKIFKMDIipkltlkkfkfkLAKKInnRKIRKLAF---KKVHEAFGGNIKEFVIGGAKINPDVEEFLRT 256
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 399 VvgegRCPIVDTWWQTETGGILIAPLPGATALkpGSATRPLFGIQPAIVDgegnELEGAAEGNLVIKDswPGQMRTIWGD 478
Cdd:cd05914 257 I----GFPYTIGYGMTETAPIISYSPPNRIRL--GSAGKVIDGVEVRIDS----PDPATGEGEIIVRG--PNVMKGYYKN 324
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 479 PdrfiEAYFSTF-KNTYF-TGDGARRDEDGYYWITGRVDDV-LNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQ 555
Cdd:cd05914 325 P----EATAEAFdKDGWFhTGDLGKIDAEGYLYIRGRKKEMiVLSSGKNIYPEEIEAKINNMPFVLESLVVVQEKKLVAL 400
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446925704 556 GICTFVTLQA---GVPES-----EELRKEL---ISWVRKVLGPVASPDalhwapALPKTRSGKIMR 610
Cdd:cd05914 401 AYIDPDFLDVkalKQRNIidaikWEVRDKVnqkVPNYKKISKVKIVKE------EFEKTPKGKIKR 460
|
|
| A_NRPS_ProA |
cd17656 |
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ... |
100-613 |
2.18e-18 |
|
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341311 [Multi-domain] Cd Length: 479 Bit Score: 88.69 E-value: 2.18e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 100 PSRHKIV------SYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRI 173
Cdd:cd17656 2 PDAVAVVfenqklTYRELNERSNQLARFLREKGVKKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPEYPEERRIYIM 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 174 EDSQAKLVITADSSlrAGKLLPLKENVDLALALPGTECVENVIVVYRNanpiemkpgrdlwyhliimevdancppepmka 253
Cdd:cd17656 82 LDSGVRVVLTQRHL--KSKLSFNKSTILLEDPSISQEDTSNIDYINNS-------------------------------- 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 254 EDPLFILYTSGSTGKPKGVLHTTGGYLVYVASTFKEVFDLKQDDVYW---CTADVGWITGHSYLIYGplanGTTTLMFEG 330
Cdd:cd17656 128 DDLLYIIYTSGTTGKPKGVQLEHKNMVNLLHFEREKTNINFSDKVLQfatCSFDVCYQEIFSTLLSG----GTLYIIREE 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 331 ----VPQyptwarLGHVVDKHKVSILYTaPTAIRAMMREGDSYVrESNRSSLRLLGSVGEP--INPEAWNWY-------Y 397
Cdd:cd17656 204 tkrdVEQ------LFDLVKRHNIEVVFL-PVAFLKFIFSEREFI-NRFPTCVKHIITAGEQlvITNEFKEMLhehnvhlH 275
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 398 NVVGEGRCPIVDTWwqTETGGILIAPLPGATalKPGSATRPLfgiqpaIVDGEGNELEGAAEGNLVIkdSWPGQMRTIWG 477
Cdd:cd17656 276 NHYGPSETHVVTTY--TINPEAEIPELPPIG--KPISNTWIY------ILDQEQQLQPQGIVGELYI--SGASVARGYLN 343
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 478 DP----DRFIEAYFSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIK 553
Cdd:cd17656 344 RQeltaEKFFPDPFDPNERMYRTGDLARYLPDGNIEFLGRADHQVKIRGYRIELGEIEAQLLNHPGVSEAVVLDKADDKG 423
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 554 GQGICTFVtlqagVPESEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRIL 613
Cdd:cd17656 424 EKYLCAYF-----VMEQELNISQLREYLAKQLPEYMIPSFFVPLDQLPLTPNGKVDRKAL 478
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
88-613 |
4.83e-18 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 89.07 E-value: 4.83e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 88 PHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPD 167
Cdd:PRK05691 2202 PQAPALTFAGQT------LSYAELDARANRLARALRERGVGPQVRVGLALERSLEMVVGLLAILKAGGAYVPLDPEYPLE 2275
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 168 SLASRIEDSQAKLVITADSSLRAGKLLPlkenvdlalALPGTECVENVIVVYRNANPIEMkPGRDLWYHLIimevdancp 247
Cdd:PRK05691 2276 RLHYMIEDSGIGLLLSDRALFEALGELP---------AGVARWCLEDDAAALAAYSDAPL-PFLSLPQHQA--------- 2336
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 248 pepmkaedplFILYTSGSTGKPKGVLHTTGGYLVYVASTFKEvFDLKQDDvywCTAdvgwitgHSYLI---------YGP 318
Cdd:PRK05691 2337 ----------YLIYTSGSTGKPKGVVVSHGEIAMHCQAVIER-FGMRADD---CEL-------HFYSInfdaaserlLVP 2395
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 319 LANGT-TTLMFEGvpqypTWA--RLGHVVDKHKVSILYTAPtairammregdSY--------VRESNRSSLRLLGSVGEP 387
Cdd:PRK05691 2396 LLCGArVVLRAQG-----QWGaeEICQLIREQQVSILGFTP-----------SYgsqlaqwlAGQGEQLPVRMCITGGEA 2459
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 388 INPEAWN---------WYYNVVGEgrcpivdtwwqTETggiLIAPL--PGATALKPGSATRP---LFGIQPA-IVDGEGN 452
Cdd:PRK05691 2460 LTGEHLQrirqafapqLFFNAYGP-----------TET---VVMPLacLAPEQLEEGAASVPigrVVGARVAyILDADLA 2525
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 453 ELEGAAEGNLVIKDSwpGQMRTIWGDPD----RFIEAYFSTFK-NTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGT 527
Cdd:PRK05691 2526 LVPQGATGELYVGGA--GLAQGYHDRPGltaeRFVADPFAADGgRLYRTGDLVRLRADGLVEYVGRIDHQVKIRGFRIEL 2603
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 528 AEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESE--ELRKELISWVRKVLGPVASPDALHWAPALPKTRS 605
Cdd:PRK05691 2604 GEIESRLLEHPAVREAVVLALDTPSGKQLAGYLVSAVAGQDDEAqaALREALKAHLKQQLPDYMVPAHLILLDSLPLTAN 2683
|
....*...
gi 446925704 606 GKIMRRIL 613
Cdd:PRK05691 2684 GKLDRRAL 2691
|
|
| FACL_like_5 |
cd05924 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
254-607 |
3.44e-17 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341248 [Multi-domain] Cd Length: 364 Bit Score: 83.59 E-value: 3.44e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 254 EDPLFILYTSGSTGKPKGVLHTTGGylVYVAStfKEVFDL---KQDDVYWCTADVGWITGHSYLIYGPLANGTTTlmfeg 330
Cdd:cd05924 3 ADDLYILYTGGTTGMPKGVMWRQED--IFRML--MGGADFgtgEFTPSEDAHKAAAAAAGTVMFPAPPLMHGTGS----- 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 331 vpqyptWARLGHVVDKHKVSIL---YTAPTAIRAMMRE--------GDSYVR------ESNR----SSLRLLGSVGEPIN 389
Cdd:cd05924 74 ------WTAFGGLLGGQTVVLPddrFDPEEVWRTIEKHkvtsmtivGDAMARplidalRDAGpydlSSLFAISSGGALLS 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 390 PEawnwyynvVGEGRCP------IVDTWWQTETGGILIAPlpgATALKPGSATRPLFGIQPAIVDGEGNELEGAAEGnlv 463
Cdd:cd05924 148 PE--------VKQGLLElvpnitLVDAFGSSETGFTGSGH---SAGSGPETGPFTRANPDTVVLDDDGRVVPPGSGG--- 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 464 ikDSWPGQMRTI----WGDPDRFIEAYFSTFKNTY-FTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHE 538
Cdd:cd05924 214 --VGWIARRGHIplgyYGDEAKTAETFPEVDGVRYaVPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKSHP 291
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 446925704 539 AVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEElrkELISWVRKVLGPVASPDALHWAPALPKTRSGK 607
Cdd:cd05924 292 AVYDVLVVGRPDERWGQEVVAVVQLREGAGVDLE---ELREHCRTRIARYKLPKQVVFVDEIERSPAGK 357
|
|
| PtmA |
cd17636 |
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ... |
255-606 |
6.63e-17 |
|
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341291 [Multi-domain] Cd Length: 331 Bit Score: 82.35 E-value: 6.63e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 255 DPLFILYTSGSTGKPKGVLHTTGGyLVYVAstfkevfdlkqddvyWCTADVGWITGHS-YLIYGPLAN------GTTTLM 327
Cdd:cd17636 1 DPVLAIYTAAFSGRPNGALLSHQA-LLAQA---------------LVLAVLQAIDEGTvFLNSGPLFHigtlmfTLATFH 64
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 328 FEG----VPQYPTwARLGHVVDKHKVSILYTAPTAIRAMmregdsyvRESNRSSLRLLGSVGEPINPEAWN--------- 394
Cdd:cd17636 65 AGGtnvfVRRVDA-EEVLELIEAERCTHAFLLPPTIDQI--------VELNADGLYDLSSLRSSPAAPEWNdmatvdtsp 135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 395 WYYNVVGEGrcpivdtwwQTETGGILIAPLPGATALkpGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDswPGQMRT 474
Cdd:cd17636 136 WGRKPGGYG---------QTEVMGLATFAALGGGAI--GGAGRPSPLVQVRILDEDGREVPDGEVGEIVARG--PTVMAG 202
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 475 IWGDPDrfIEAYFSTFkNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKG 554
Cdd:cd17636 203 YWNRPE--VNARRTRG-GWHHTNDLGRREPDGSLSFVGPKTRMIKSGAENIYPAEVERCLRQHPAVADAAVIGVPDPRWA 279
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|..
gi 446925704 555 QGICTFVTLQAGVPESEElrkELISWVRKVLGPVASPDALHWAPALPKTRSG 606
Cdd:cd17636 280 QSVKAIVVLKPGASVTEA---ELIEHCRARIASYKKPKSVEFADALPRTAGG 328
|
|
| PRK08279 |
PRK08279 |
long-chain-acyl-CoA synthetase; Validated |
80-302 |
1.33e-16 |
|
long-chain-acyl-CoA synthetase; Validated
Pssm-ID: 236217 [Multi-domain] Cd Length: 600 Bit Score: 83.39 E-value: 1.33e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIWEGDHpsrhkiVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCV 159
Cdd:PRK08279 43 FEEAAARHPDRPALLFEDQS------ISYAELNARANRYAHWAAARGVGKGDVVALLMENRPEYLAAWLGLAKLGAVVAL 116
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 160 VFGGFSPDSLASRIEDSQAKLVITADsslragKLLPLKENVDLALALPGTECVENVIvvyRNANPIEMkpgRDLwyhlii 239
Cdd:PRK08279 117 LNTQQRGAVLAHSLNLVDAKHLIVGE------ELVEAFEEARADLARPPRLWVAGGD---TLDDPEGY---EDL------ 178
|
170 180 190 200 210 220
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 446925704 240 MEVDANCPPEP------MKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYvASTFKEVFDLKQDDVYWCT 302
Cdd:PRK08279 179 AAAAAGAPTTNpasrsgVTAKDTAFYIYTSGTTGLPKAAVMSHMRWLKA-MGGFGGLLRLTPDDVLYCC 246
|
|
| A_NRPS_TubE_like |
cd05906 |
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ... |
105-616 |
3.90e-16 |
|
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341232 [Multi-domain] Cd Length: 540 Bit Score: 81.94 E-value: 3.90e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 105 IVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACarigavhcvVFGGFSPDSLAsriedsqaKLVITA 184
Cdd:cd05906 39 FQSYQDLLEDARRLAAGLRQLGLRPGDSVILQFDDNEDFIPAFWAC---------VLAGFVPAPLT--------VPPTYD 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 185 DSSLRAGKLLPLKENVDLALALPGTECVEnvivvyrnanpiEMKPGRDLWYH----LIIMEVDANCPPEPM----KAEDP 256
Cdd:cd05906 102 EPNARLRKLRHIWQLLGSPVVLTDAELVA------------EFAGLETLSGLpgirVLSIEELLDTAADHDlpqsRPDDL 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 257 LFILYTSGSTGKPKGVLHTTGGYLVYVASTfKEVFDLKQDDVY--WCTAD-VGWITgHSYL---------IYGPlangtT 324
Cdd:cd05906 170 ALLMLTSGSTGFPKAVPLTHRNILARSAGK-IQHNGLTPQDVFlnWVPLDhVGGLV-ELHLravylgcqqVHVP-----T 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 325 TLMFEGVPQyptWARLghvVDKHKVSILYtAPTAIRAMMREgdsYVRESNR-----SSLRLLGSVGEPINPEAWNWYYNV 399
Cdd:cd05906 243 EEILADPLR---WLDL---IDRYRVTITW-APNFAFALLND---LLEEIEDgtwdlSSLRYLVNAGEAVVAKTIRRLLRL 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 400 VGE-GRCP--IVDTWWQTETG-GILIAPLPGATALKPG----SATRPLFGIQPAIVDGEGNELEGAAEGNLVIKdswpGQ 471
Cdd:cd05906 313 LEPyGLPPdaIRPAFGMTETCsGVIYSRSFPTYDHSQAlefvSLGRPIPGVSMRIVDDEGQLLPEGEVGRLQVR----GP 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 472 MRTI--WGDPdrfiEAYFSTF-KNTYF-TGDGARRDeDGYYWITGRVDDVLNVSGHRLGTAEIESALvshEAVAE----- 542
Cdd:cd05906 389 VVTKgyYNNP----EANAEAFtEDGWFrTGDLGFLD-NGNLTITGRTKDTIIVNGVNYYSHEIEAAV---EEVPGvepsf 460
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 543 --AAVVGMPHDIKGQGICTFVTLQAGVPESEELRKELISWVRKVLGpvASPDALhwAP----ALPKTRSGKIMRRILRKI 616
Cdd:cd05906 461 taAFAVRDPGAETEELAIFFVPEYDLQDALSETLRAIRSVVSREVG--VSPAYL--IPlpkeEIPKTSLGKIQRSKLKAA 536
|
|
| A_NRPS_ApnA-like |
cd17644 |
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ... |
254-613 |
5.20e-16 |
|
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341299 [Multi-domain] Cd Length: 465 Bit Score: 80.94 E-value: 5.20e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 254 EDPLFILYTSGSTGKPKGVL--------HTTGGYLVYVASTFKEV-------FDLKQDDVYwctadVGWITGHSYLIygp 318
Cdd:cd17644 106 ENLAYVIYTSGSTGKPKGVMiehqslvnLSHGLIKEYGITSSDRVlqfasiaFDVAAEEIY-----VTLLSGATLVL--- 177
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 319 langTTTLMFEGVPQYPTWARlghvvdKHKVSILYTAPTAIRAMMREGdSYVRESNRSSLRLLGSVGEPINPEAWN-W-- 395
Cdd:cd17644 178 ----RPEEMRSSLEDFVQYIQ------QWQLTVLSLPPAYWHLLVLEL-LLSTIDLPSSLRLVIVGGEAVQPELVRqWqk 246
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 396 -------YYNVVGEGRCPIVDTWWqtetggiLIAPLPGATALKPgSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDSw 468
Cdd:cd17644 247 nvgnfiqLINVYGPTEATIAATVC-------RLTQLTERNITSV-PIGRPIANTQVYILDENLQPVPVGVPGELHIGGV- 317
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 469 pGQMRTIWGDPD----RFIEAYF--STFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAE 542
Cdd:cd17644 318 -GLARGYLNRPEltaeKFISHPFnsSESERLYKTGDLARYLPDGNIEYLGRIDNQVKIRGFRIELGEIEAVLSQHNDVKT 396
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 446925704 543 AAVVGMPHDIKGQGICTFVtlqagVPESEE--LRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRIL 613
Cdd:cd17644 397 AVVIVREDQPGNKRLVAYI-----VPHYEEspSTVELRQFLKAKLPDYMIPSAFVVLEELPLTPNGKIDRRAL 464
|
|
| PRK13383 |
PRK13383 |
acyl-CoA synthetase; Provisional |
87-613 |
5.72e-16 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139531 [Multi-domain] Cd Length: 516 Bit Score: 81.20 E-value: 5.72e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 87 HPHKPAIIWEGDHPSRHKIV------SYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVV 160
Cdd:PRK13383 36 NPYTLLAVTAARWPGRTAIIdddgalSYRELQRATESLARRLTRDGVAPGRAVGVMCRNGRGFVTAVFAVGLLGADVVPI 115
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 161 FGGFSPDSLASRIEDSQAKLVITADSSlrAGKLLPLKENVdlALALPGTECVENVivvyrNANPIEMKPGRdlwyhliim 240
Cdd:PRK13383 116 STEFRSDALAAALRAHHISTVVADNEF--AERIAGADDAV--AVIDPATAGAEES-----GGRPAVAAPGR--------- 177
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 241 evdancppepmkaedplFILYTSGSTGKPKGVLHT---TGGYLVYVasTFKEVFDLKQDDVYWCTADVGWITGHSYLIYg 317
Cdd:PRK13383 178 -----------------IVLLTSGTTGKPKGVPRApqlRSAVGVWV--TILDRTRLRTGSRISVAMPMFHGLGLGMLML- 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 318 PLANGTTTLMFEGVPQYPTWARlghvVDKHKVSILYTAPTAIrAMMREGDSYVRESNR-SSLRLLGSVGEPINPEAWNWY 396
Cdd:PRK13383 238 TIALGGTVLTHRHFDAEAALAQ----ASLHRADAFTAVPVVL-ARILELPPRVRARNPlPQLRVVMSSGDRLDPTLGQRF 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 397 YNVVGEgrcPIVDTWWQTETG-GILIAPLPGATAlkPGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDSWPGQmRTI 475
Cdd:PRK13383 313 MDTYGD---ILYNGYGSTEVGiGALATPADLRDA--PETVGKPVAGCPVRILDRNNRPVGPRVTGRIFVGGELAGT-RYT 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 476 WGDPDRFIEAYFSTfkntyftGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQ 555
Cdd:PRK13383 387 DGGGKAVVDGMTST-------GDMGYLDNAGRLFIVGREDDMIISGGENVYPRAVENALAAHPAVADNAVIGVPDERFGH 459
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 446925704 556 GICTFVTLQAGVP-ESEELRKELISWVRKvlgpVASPDALHWAPALPKTRSGKIMRRIL 613
Cdd:PRK13383 460 RLAAFVVLHPGSGvDAAQLRDYLKDRVSR----FEQPRDINIVSSIPRNPTGKVLRKEL 514
|
|
| PRK13388 |
PRK13388 |
acyl-CoA synthetase; Provisional |
247-614 |
7.53e-16 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237374 [Multi-domain] Cd Length: 540 Bit Score: 80.84 E-value: 7.53e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 247 PPEPMKAEDPLFILYTSGSTGKPKGVLhTTGGYLVYVASTFKEVFDLKQDDVYWCTADVGwitgHSYLIY---GP-LANG 322
Cdd:PRK13388 143 PHREVDAMDPFMLIFTSGTTGAPKAVR-CSHGRLAFAGRALTERFGLTRDDVCYVSMPLF----HSNAVMagwAPaVASG 217
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 323 TTtlmfegVPQYPTWARLGHVVDkhkvsilytaptaIRammREGDSYvresnrsslrlLGSVGEPI-----NPEAWNWYY 397
Cdd:PRK13388 218 AA------VALPAKFSASGFLDD-------------VR---RYGATY-----------FNYVGKPLayilaTPERPDDAD 264
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 398 N--VVGEG---------------RCPIVDTWWQTETGGILIAPlPGAtalKPGSATRPLFGIqpAIVDGEGNELEGAAE- 459
Cdd:PRK13388 265 NplRVAFGneasprdiaefsrrfGCQVEDGYGSSEGAVIVVRE-PGT---PPGSIGRGAPGV--AIYNPETLTECAVARf 338
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 460 ---GNLVIKDSWPGQMRTIWGdPDRFiEAYFSTFKNT--------YFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTA 528
Cdd:PRK13388 339 dahGALLNADEAIGELVNTAG-AGFF-EGYYNNPEATaermrhgmYWSGDLAYRDADGWIYFAGRTADWMRVDGENLSAA 416
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 529 EIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVP-ESEELRKELISwvRKVLGPVASPDALHWAPALPKTRSGK 607
Cdd:PRK13388 417 PIERILLRHPAINRVAVYAVPDERVGDQVMAALVLRDGATfDPDAFAAFLAA--QPDLGTKAWPRYVRIAADLPSTATNK 494
|
....*..
gi 446925704 608 IMRRILR 614
Cdd:PRK13388 495 VLKRELI 501
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
258-646 |
1.31e-15 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 81.37 E-value: 1.31e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 258 FILYTSGSTGKPKGVLHTTGGYLVYVASTFKeVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFEGVPQYPTw 337
Cdd:PRK05691 3873 YVIYTSGSTGLPKGVMVEQRGMLNNQLSKVP-YLALSEADVIAQTASQSFDISVWQFLAAPLFGARVEIVPNAIAHDPQ- 3950
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 338 ARLGHVVDKhKVSILYTAPTAIRAMMREgdsyvRESNRSSLRLLGSVGEPINPE-AWNWY--------YNVVGEGRCPIV 408
Cdd:PRK05691 3951 GLLAHVQAQ-GITVLESVPSLIQGMLAE-----DRQALDGLRWMLPTGEAMPPElARQWLqrypqiglVNAYGPAECSDD 4024
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 409 DTWWQTETGGILIAPLPGATalkPGSATRpLFgiqpaIVDGEGNELEGAAEGNLVIKDSWPGqmRTIWGDPDR----FIE 484
Cdd:PRK05691 4025 VAFFRVDLASTRGSYLPIGS---PTDNNR-LY-----LLDEALELVPLGAVGELCVAGTGVG--RGYVGDPLRtalaFVP 4093
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 485 AYFSTF-KNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAvVGMPHDIKGQGICTFVTL 563
Cdd:PRK05691 4094 HPFGAPgERLYRTGDLARRRSDGVLEYVGRIDHQVKIRGYRIELGEIEARLHEQAEVREAA-VAVQEGVNGKHLVGYLVP 4172
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 564 QAGVPESEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILrkiAANELDSLGDTSTLAEPAVVDQLITTV 643
Cdd:PRK05691 4173 HQTVLAQGALLERIKQRLRAELPDYMVPLHWLWLDRLPLNANGKLDRKAL---PALDIGQLQSQAYLAPRNELEQTLATI 4249
|
...
gi 446925704 644 YPD 646
Cdd:PRK05691 4250 WAD 4252
|
|
| PRK09274 |
PRK09274 |
peptide synthase; Provisional |
82-577 |
1.39e-15 |
|
peptide synthase; Provisional
Pssm-ID: 236443 [Multi-domain] Cd Length: 552 Bit Score: 79.94 E-value: 1.39e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 82 RHL----KEHPHKPAII----WEGDHPSRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLympMVTEAA--IA-MLAC 150
Cdd:PRK09274 10 RHLpraaQERPDQLAVAvpggRGADGKLAYDELSFAELDARSDAIAHGLNAAGIGRGMRAVL---MVTPSLefFAlTFAL 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 151 ARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITADSSLRAGKLLPLKEnvdlalalpgtECVENVIVVYRNANPiemkPG 230
Cdd:PRK09274 87 FKAGAVPVLVDPGMGIKNLKQCLAEAQPDAFIGIPKAHLARRLFGWGK-----------PSVRRLVTVGGRLLW----GG 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 231 RDLwYHLIIMEVDANCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVaSTFKEVFDLKQDDVYWCTADVgwitg 310
Cdd:PRK09274 152 TTL-ATLLRDGAAAPFPMADLAPDDMAAILFTSGSTGTPKGVVYTHGMFEAQI-EALREDYGIEPGEIDLPTFPL----- 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 311 hsYLIYGPlANGTTTLMFEGVPQYPTWA---RLGHVVDKHKVSILYTAPTAIRAMMREGDSyvRESNRSSLRLLGSVGEP 387
Cdd:PRK09274 225 --FALFGP-ALGMTSVIPDMDPTRPATVdpaKLFAAIERYGVTNLFGSPALLERLGRYGEA--NGIKLPSLRRVISAGAP 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 388 INPEAWNWY----------YNVVG----------EGRCPIVDTWWQTETG-GILIAplpgatalkpgsatRPLFGIQP-- 444
Cdd:PRK09274 300 VPIAVIERFramlppdaeiLTPYGatealpissiESREILFATRAATDNGaGICVG--------------RPVDGVEVri 365
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 445 -AIVDGEGNELEGAAE------GNLVIKdswpGQMRT--------------IWgDPDRFIeayfstfknTYFTGDGARRD 503
Cdd:PRK09274 366 iAISDAPIPEWDDALRlatgeiGEIVVA----GPMVTrsyynrpeatrlakIP-DGQGDV---------WHRMGDLGYLD 431
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446925704 504 EDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPhdIKGQ---GICtfVTLQAGV-PESEELRKEL 577
Cdd:PRK09274 432 AQGRLWFCGRKAHRVETAGGTLYTIPCERIFNTHPGVKRSALVGVG--VPGAqrpVLC--VELEPGVaCSKSALYQEL 505
|
|
| A_NRPS_ACVS-like |
cd17648 |
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ... |
106-613 |
5.16e-14 |
|
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.
Pssm-ID: 341303 [Multi-domain] Cd Length: 453 Bit Score: 74.74 E-value: 5.16e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 106 VSYKELHDEVCRFANVLKKYGIGKGD-RVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITa 184
Cdd:cd17648 13 LTYRELNERANRLAHYLLSVAEIRPDdLVGLVLDKSELMIIAILAVWKAGAAYVPIDPSYPDERIQFILEDTGARVVIT- 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 185 DSSlragkllplkenvDLAlalpgtecvenvivvyrnanpiemkpgrdlwyhliimevdancppepmkaedplFILYTSG 264
Cdd:cd17648 92 NST-------------DLA------------------------------------------------------YAIYTSG 104
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 265 STGKPKGVLHTTGGyLVYVASTFKEVFDLKqddvywCTADVGWITGHSYlIYGP--------LANGTTTLMF--EGVPQY 334
Cdd:cd17648 105 TTGKPKGVLVEHGS-VVNLRTSLSERYFGR------DNGDEAVLFFSNY-VFDFfveqmtlaLLNGQKLVVPpdEMRFDP 176
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 335 PTWARLGhvvDKHKVSILYTAPTAIrammregdSYVRESNRSSLRLLGSVGEPINPEAWNwyyNVVGEGRCPIVDTWWQT 414
Cdd:cd17648 177 DRFYAYI---NREKVTYLSGTPSVL--------QQYDLARLPHLKRVDAAGEEFTAPVFE---KLRSRFAGLIINAYGPT 242
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 415 ETG-GILIAPLPGATALKpGSATRPLFGIQPAIVDGEGNELEGAAEGNLVIKDSwpGQMRTIWGDP----DRFIEAYFST 489
Cdd:cd17648 243 ETTvTNHKRFFPGDQRFD-KSLGRPVRNTKCYVLNDAMKRVPVGAVGELYLGGD--GVARGYLNRPeltaERFLPNPFQT 319
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 490 --------FKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQG----- 556
Cdd:cd17648 320 eqerargrNARLYKTGDLVRWLPSGELEYLGRNDFQVKIRGQRIEPGEVEAALASYPGVRECAVVAKEDASQAQSriqky 399
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 446925704 557 -ICTFVTLQAGVPESeelrkELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRIL 613
Cdd:cd17648 400 lVGYYLPEPGHVPES-----DLLSFLRAKLPRYMVPARLVRLEGIPVTINGKLDVRAL 452
|
|
| PRK07824 |
PRK07824 |
o-succinylbenzoate--CoA ligase; |
478-615 |
1.13e-13 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 236108 [Multi-domain] Cd Length: 358 Bit Score: 72.77 E-value: 1.13e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 478 DPDRFIEAyfstfknTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGI 557
Cdd:PRK07824 226 DPDPFAEP-------GWFRTDDLGALDDGVLTVLGRADDAISTGGLTVLPQVVEAALATHPAVADCAVFGLPDDRLGQRV 298
|
90 100 110 120 130
....*....|....*....|....*....|....*....|....*....|....*....
gi 446925704 558 CTFVTLQAG-VPESEELRkeliSWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRK 615
Cdd:PRK07824 299 VAAVVGDGGpAPTLEALR----AHVARTLDRTAAPRELHVVDELPRRGIGKVDRRALVR 353
|
|
| A_NRPS_acs4 |
cd17654 |
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal ... |
88-608 |
2.94e-13 |
|
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains acyl-CoA synthethase family member 4, also known as 2-aminoadipic 6-semialdehyde dehydrogenase or aminoadipate-semialdehyde dehydrogenase, most of which are uncharacterized. Acyl-CoA synthetase catalyzes the initial reaction in fatty acid metabolism, by forming a thioester with CoA. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341309 [Multi-domain] Cd Length: 449 Bit Score: 72.12 E-value: 2.94e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 88 PHKPAIIWegDHPSRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHcVVFGGFSPD 167
Cdd:cd17654 1 PDRPALII--DQTTSDTTVSYADLAEKISNLSNFLRKKFQTEERAIGLRCDRGTESPVAILAILFLGAAY-APIDPASPE 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 168 SLASRIEDSQAKLVITADSSLRAGKLLPLKENVDLALALPGTECvenvivvyrnanpiemkpgrdlwyhliimevdancp 247
Cdd:cd17654 78 QRSLTVMKKCHVSYLLQNKELDNAPLSFTPEHRHFNIRTDECLA------------------------------------ 121
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 248 pepmkaedplFILYTSGSTGKPKGVLhttggylVYVAS------TFKEVFDLKQDDVYWCTA----DVGWITghsylIYG 317
Cdd:cd17654 122 ----------YVIHTSGTTGTPKIVA-------VPHKCilpniqHFRSLFNITSEDILFLTSpltfDPSVVE-----IFL 179
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 318 PLANGTTTLMFE-GVPQYPtwARLGHVVDK-HKVSILYTAPTAIRammREGDSYVRESNRS---SLRLLGSVGE--PINP 390
Cdd:cd17654 180 SLSSGATLLIVPtSVKVLP--SKLADILFKrHRITVLQATPTLFR---RFGSQSIKSTVLSatsSLRVLALGGEpfPSLV 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 391 EAWNW--------YYNVVGEGRCPIVDTWWQTETGGiliAPLPGATalkpgsatrPLFGIQPAIVDGEGNELEGAAEGNL 462
Cdd:cd17654 255 ILSSWrgkgnrtrIFNIYGITEVSCWALAYKVPEED---SPVQLGS---------PLLGTVIEVRDQNGSEGTGQVFLGG 322
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 463 VIKDSwpgqmrtiwgdpdrFIEAYFSTFKNT-YFTGDGARRdEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVA 541
Cdd:cd17654 323 LNRVC--------------ILDDEVTVPKGTmRATGDFVTV-KDGELFFLGRKDSQIKRRGKRINLDLIQQVIESCLGVE 387
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446925704 542 EAAVvgMPHDiKGQGICTFVTLQAGVPESEELRKELISwvrkvlgPVASPDALHWAPALPKTRSGKI 608
Cdd:cd17654 388 SCAV--TLSD-QQRLIAFIVGESSSSRIHKELQLTLLS-------SHAIPDTFVQIDKLPLTSHGKV 444
|
|
| LC_FACS_like |
cd17640 |
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ... |
104-577 |
1.59e-12 |
|
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341295 [Multi-domain] Cd Length: 468 Bit Score: 70.08 E-value: 1.59e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 104 KIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHcVVFGGFSP-DSLASRIEDSQAKLVI 182
Cdd:cd17640 4 KRITYKDLYQEILDFAAGLRSLGVKAGEKVALFADNSPRWLIADQGIMALGAVD-VVRGSDSSvEELLYILNHSESVALV 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 183 tadsslragkllplkenvdlalalpgtecVENvivvyrnanpiemkpgrdlwyhliimevdancppepmKAEDPLFILYT 262
Cdd:cd17640 83 -----------------------------VEN-------------------------------------DSDDLATIIYT 96
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 263 SGSTGKPKGVLHTTGGyLVYVASTFKEVFDLKQDDVYWCTADVgWitgHSYliygplangtttlmfEGVPQYPTWARlgh 342
Cdd:cd17640 97 SGTTGNPKGVMLTHAN-LLHQIRSLSDIVPPQPGDRFLSILPI-W---HSY---------------ERSAEYFIFAC--- 153
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 343 vvdkhKVSILYTAPTAIRAMM------------------REG-DSYVRESNRSSLRLLG---SVGE---PIN-----PEA 392
Cdd:cd17640 154 -----GCSQAYTSIRTLKDDLkrvkphyivsvprlweslYSGiQKQVSKSSPIKQFLFLfflSGGIfkfGISgggalPPH 228
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 393 WNWYYNVVGegrCPIVDTWWQTETGGILIAPLPGATALkpGSATRPLFGIQPAIVDGEGNE-LEGAAEGNLVIKDswPGQ 471
Cdd:cd17640 229 VDTFFEAIG---IEVLNGYGLTETSPVVSARRLKCNVR--GSVGRPLPGTEIKIVDPEGNVvLPPGEKGIVWVRG--PQV 301
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 472 MRTIWGDPDRFIEAYFStfkNTYF-TGDGARRDEDGYYWITGRVDD--VLNvSGHRLGTAEIESALVSHEAVAEAAVVGm 548
Cdd:cd17640 302 MKGYYKNPEATSKVLDS---DGWFnTGDLGWLTCGGELVLTGRAKDtiVLS-NGENVEPQPIEEALMRSPFIEQIMVVG- 376
|
490 500
....*....|....*....|....*....
gi 446925704 549 pHDIKGQGICTfvtlqagVPESEELRKEL 577
Cdd:cd17640 377 -QDQKRLGALI-------VPNFEELEKWA 397
|
|
| PLN02387 |
PLN02387 |
long-chain-fatty-acid-CoA ligase family protein |
107-320 |
1.64e-12 |
|
long-chain-fatty-acid-CoA ligase family protein
Pssm-ID: 215217 [Multi-domain] Cd Length: 696 Bit Score: 70.53 E-value: 1.64e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 107 SYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITADS 186
Cdd:PLN02387 108 TYGQVFERVCNFASGLVALGHNKEERVAIFADTRAEWLIALQGCFRQNITVVTIYASLGEEALCHSLNETEVTTVICDSK 187
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 187 SLRagKLLPLKENVdlalalpgtECVENVIvvYRNANPIEMKPGRDLWYHLIIME------------VDANCPpepmKAE 254
Cdd:PLN02387 188 QLK--KLIDISSQL---------ETVKRVI--YMDDEGVDSDSSLSGSSNWTVSSfseveklgkenpVDPDLP----SPN 250
|
170 180 190 200 210 220
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446925704 255 DPLFILYTSGSTGKPKGVLHTTGGYLVYVASTFKEVFDLKQDDVywctadvgwitghsYLIYGPLA 320
Cdd:PLN02387 251 DIAVIMYTSGSTGLPKGVMMTHGNIVATVAGVMTVVPKLGKNDV--------------YLAYLPLA 302
|
|
| PLN02860 |
PLN02860 |
o-succinylbenzoate-CoA ligase |
109-630 |
7.10e-12 |
|
o-succinylbenzoate-CoA ligase
Pssm-ID: 215464 [Multi-domain] Cd Length: 563 Bit Score: 68.29 E-value: 7.10e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 109 KELHDEVCRFANVLKKYGIGKGDRVvlympmvteaAIA----------MLACARIGAVHCVVFGGFSPDSLASRIEDSQA 178
Cdd:PLN02860 36 HEFVDGVLSLAAGLLRLGLRNGDVV----------AIAalnsdlylewLLAVACAGGIVAPLNYRWSFEEAKSAMLLVRP 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 179 KLVITaDSS-------LRAGKLLPLKENVdlalaLPGTECVENVIVVYrNANPIEMkpgrdLWYHLIImevdancPPEPM 251
Cdd:PLN02860 106 VMLVT-DETcsswyeeLQNDRLPSLMWQV-----FLESPSSSVFIFLN-SFLTTEM-----LKQRALG-------TTELD 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 252 KA---EDPLFILYTSGSTGKPKGVL--HTTggyLVyVASTFK-EVFDLKQDDVYWCTADVGWITGhsyliygpLANGTTT 325
Cdd:PLN02860 167 YAwapDDAVLICFTSGTTGRPKGVTisHSA---LI-VQSLAKiAIVGYGEDDVYLHTAPLCHIGG--------LSSALAM 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 326 LMFEG----VPQYPTWARLgHVVDKHKVSILYTAPtairAMMREGDSYVRES-----NRSSLRLL---GSVGEPINPEAW 393
Cdd:PLN02860 235 LMVGAchvlLPKFDAKAAL-QAIKQHNVTSMITVP----AMMADLISLTRKSmtwkvFPSVRKILnggGSLSSRLLPDAK 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 394 NWYynvvgeGRCPIVDTWWQTETGGIL-IAPLPGATALKPGSATRPLFGIQPAIVD-GEGN---------EL-----EGA 457
Cdd:PLN02860 310 KLF------PNAKLFSAYGMTEACSSLtFMTLHDPTLESPKQTLQTVNQTKSSSVHqPQGVcvgkpaphvELkigldESS 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 458 AEGNLVIKDswPGQMRTIWGDP-----DRFIEAYFSTfkntyftGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIES 532
Cdd:PLN02860 384 RVGRILTRG--PHVMLGYWGQNsetasVLSNDGWLDT-------GDIGWIDKAGNLWLIGRSNDRIKTGGENVYPEEVEA 454
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 533 ALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAG----VPESEELRKELiswvrkvlgpVASPDALH------------- 595
Cdd:PLN02860 455 VLSQHPGVASVVVVGVPDSRLTEMVVACVRLRDGwiwsDNEKENAKKNL----------TLSSETLRhhcreknlsrfki 524
|
570 580 590 600
....*....|....*....|....*....|....*....|.
gi 446925704 596 ------WAPALPKTRSGKIMRRILRKIAANELDSLgdTSTL 630
Cdd:PLN02860 525 pklfvqWRKPFPLTTTGKIRRDEVRREVLSHLQSL--PSNL 563
|
|
| FATP_chFAT1_like |
cd05937 |
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ... |
102-615 |
1.22e-11 |
|
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.
Pssm-ID: 341260 [Multi-domain] Cd Length: 468 Bit Score: 67.46 E-value: 1.22e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 102 RHKIVSYKELHDEVCRFANVLKK-YGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKL 180
Cdd:cd05937 2 EGKTWTYSETYDLVLRYAHWLHDdLGVQAGDFVAIDLTNSPEFVFLWLGLWSIGAAPAFINYNLSGDPLIHCLKLSGSRF 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 181 VITADsslragkllplkenvdlalalpgtecvenvivvyrnanpiemkpgrdlwyhliimevdancppepmkaEDPLFIL 260
Cdd:cd05937 82 VIVDP--------------------------------------------------------------------DDPAILI 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 261 YTSGSTGKPKGVLHTTGGYLVyVASTFKEVFDLKQDD-VYWC----------TADVGWITGHSYLIYGP----------- 318
Cdd:cd05937 94 YTSGTTGLPKAAAISWRRTLV-TSNLLSHDLNLKNGDrTYTCmplyhgtaafLGACNCLMSGGTLALSRkfsasqfwkdv 172
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 319 LANGTTTLMFEG--------VPQYPTwarlghvvDK-HKVSILY---TAP---TAIRAmmREGDSYVRESNRSSLRLLGS 383
Cdd:cd05937 173 RDSGATIIQYVGelcryllsTPPSPY--------DRdHKVRVAWgngLRPdiwERFRE--RFNVPEIGEFYAATEGVFAL 242
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 384 vgepinpeaWNWYYNVVGEGRCPIVDTWWQ-------------TETGGILIAPlpgatalKPGSATR-PLfgiqpaivdG 449
Cdd:cd05937 243 ---------TNHNVGDFGAGAIGHHGLIRRwkfenqvvlvkmdPETDDPIRDP-------KTGFCVRaPV---------G 297
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 450 EGNELEGAAegNLVIKDSWPGqmrtIWGDPD----RFIEAYFSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRL 525
Cdd:cd05937 298 EPGEMLGRV--PFKNREAFQG----YLHNEDatesKLVRDVFRKGDIYFRTGDLLRQDADGRWYFLDRLGDTFRWKSENV 371
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 526 GTAEIESALVSHEAVAEAAVVGMP---HDikGQGICTFVTLQ-AGVPESEELRKELISWVRKVLGPVASPDALHWAPALP 601
Cdd:cd05937 372 STTEVADVLGAHPDIAEANVYGVKvpgHD--GRAGCAAITLEeSSAVPTEFTKSLLASLARKNLPSYAVPLFLRLTEEVA 449
|
570
....*....|....
gi 446925704 602 KTRSGKIMRRILRK 615
Cdd:cd05937 450 TTDNHKQQKGVLRD 463
|
|
| PRK07445 |
PRK07445 |
O-succinylbenzoic acid--CoA ligase; Reviewed |
494-621 |
2.10e-11 |
|
O-succinylbenzoic acid--CoA ligase; Reviewed
Pssm-ID: 236019 [Multi-domain] Cd Length: 452 Bit Score: 66.56 E-value: 2.10e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 494 YFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEEL 573
Cdd:PRK07445 326 FETDDLGYLDAQGYLHILGRNSQKIITGGENVYPAEVEAAILATGLVQDVCVLGLPDPHWGEVVTAIYVPKDPSISLEEL 405
|
90 100 110 120
....*....|....*....|....*....|....*....|....*...
gi 446925704 574 RkeliSWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRKIAANEL 621
Cdd:PRK07445 406 K----TAIKDQLSPFKQPKHWIPVPQLPRNPQGKINRQQLQQIAVQRL 449
|
|
| hsFATP4_like |
cd05939 |
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ... |
107-615 |
2.20e-11 |
|
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341262 [Multi-domain] Cd Length: 474 Bit Score: 66.68 E-value: 2.20e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 107 SYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITAds 186
Cdd:cd05939 5 TFRELNEYSNKVANFFQAQGYRSGDVVALFMENRLEFVALWLGLAKIGVETALINSNLRLESLLHCITVSKAKALIFN-- 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 187 slragKLLPLKEnvDLALALPGTECVEnvivvyrnanpiemkpgrdlwyhliimevdancppepmkAEDPLFILYTSGST 266
Cdd:cd05939 83 -----LLDPLLT--QSSTEPPSQDDVN---------------------------------------FRDKLFYIYTSGTT 116
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 267 GKPKGVLHTTGGYLVYVASTFKeVFDLKQDDV-YWC-----TAdvGWITGhsylIYGPLANGTTTLMFEGVPQYPTWARl 340
Cdd:cd05939 117 GLPKAAVIVHSRYYRIAAGAYY-AFGMRPEDVvYDClplyhSA--GGIMG----VGQALLHGSTVVIRKKFSASNFWDD- 188
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 341 ghvVDKHKVSILYTAPTAIRAMMREgdSYVRESNRSSLRLLgsVGEPINPEAWNWYYNVVG-----------EGRCPIVD 409
Cdd:cd05939 189 ---CVKYNCTIVQYIGEICRYLLAQ--PPSEEEQKHNVRLA--VGNGLRPQIWEQFVRRFGipqigefygatEGNSSLVN 261
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 410 TWWQTETGGILIAPLPGatalkpgsatrpLFGIQPAIVDGEGNELEGAAEGNLV-IKDSWPGQM--RTIWGDPDRFIEAY 486
Cdd:cd05939 262 IDNHVGACGFNSRILPS------------VYPIRLIKVDEDTGELIRDSDGLCIpCQPGEPGLLvgKIIQNDPLRRFDGY 329
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 487 FS-----------TFKN---TYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVG--MPH 550
Cdd:cd05939 330 VNegatnkkiardVFKKgdsAFLSGDVLVMDELGYLYFKDRTGDTFRWKGENVSTTEVEGILSNVLGLEDVVVYGveVPG 409
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 446925704 551 DIKGQGICTFVtlqagVPESEELRKELISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRK 615
Cdd:cd05939 410 VEGRAGMAAIV-----DPERKVDLDRFSAVLAKSLPPYARPQFIRLLPEVDKTGTFKLQKTDLQK 469
|
|
| FACL_like_1 |
cd05910 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
252-578 |
4.63e-11 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341236 [Multi-domain] Cd Length: 457 Bit Score: 65.56 E-value: 4.63e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 252 KAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVaSTFKEVFDLKQDDVYWCTADVgwitghsYLIYGPlANGTTTLMFEGV 331
Cdd:cd05910 83 KADEPAAILFTSGSTGTPKGVVYRHGTFAAQI-DALRQLYGIRPGEVDLATFPL-------FALFGP-ALGLTSVIPDMD 153
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 332 PQYPTWARLGHVVD---KHKVSILYTAPTAIRAMMREGDSYVREsnRSSLRLLGSVGEPINPEAWNWYYNVVGEGrCPIV 408
Cdd:cd05910 154 PTRPARADPQKLVGairQYGVSIVFGSPALLERVARYCAQHGIT--LPSLRRVLSAGAPVPIALAARLRKMLSDE-AEIL 230
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 409 DTWWQTETGGI-------LIAPLPGATAlkPGSAT---RPLFGIQPAIV---DGEGNELEGAAE------GNLVIKdswp 469
Cdd:cd05910 231 TPYGATEALPVssigsreLLATTTAATS--GGAGTcvgRPIPGVRVRIIeidDEPIAEWDDTLElprgeiGEITVT---- 304
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 470 GQM--RTIWGDPDRFIEAYFSTFKNTYF--TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAV 545
Cdd:cd05910 305 GPTvtPTYVNRPVATALAKIDDNSEGFWhrMGDLGYLDDEGRLWFCGRKAHRVITTGGTLYTEPVERVFNTHPGVRRSAL 384
|
330 340 350
....*....|....*....|....*....|...
gi 446925704 546 VGMPHDIKGQGICTFVTLQAGVPESEELRKELI 578
Cdd:cd05910 385 VGVGKPGCQLPVLCVEPLPGTITPRARLEQELR 417
|
|
| PRK08308 |
PRK08308 |
acyl-CoA synthetase; Validated |
474-613 |
7.71e-11 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236231 [Multi-domain] Cd Length: 414 Bit Score: 64.67 E-value: 7.71e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 474 TIWGDPDRFIEAYFSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIK 553
Cdd:PRK08308 273 SAGSDENAPEEIVVKMGDKEIFTKDLGYKSERGTLHFMGRMDDVINVSGLNVYPIEVEDVMLRLPGVQEAVVYRGKDPVA 352
|
90 100 110 120 130 140
....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 554 GQGICTFVTLQAGVpESEELRKelisWVRKVLGPVASPDALHWAPALPKTRSGKIMRRIL 613
Cdd:PRK08308 353 GERVKAKVISHEEI-DPVQLRE----WCIQHLAPYQVPHEIESVTEIPKNANGKVSRKLL 407
|
|
| LC_FACS_euk1 |
cd17639 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ... |
101-575 |
1.24e-10 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.
Pssm-ID: 341294 [Multi-domain] Cd Length: 507 Bit Score: 64.16 E-value: 1.24e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 101 SRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACArigavhcvvfggfspdslasriedSQAKL 180
Cdd:cd17639 1 GEYKYMSYAEVWERVLNFGRGLVELGLKPGDKVAIFAETRAEWLITALGCW------------------------SQNIP 56
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 181 VITADSSLraGKllplkenVDLALALPGTECVenviVVYRNANPiemkpgrdlwyhliimevdancppepmkaEDPLFIL 260
Cdd:cd17639 57 IVTVYATL--GE-------DALIHSLNETECS----AIFTDGKP-----------------------------DDLACIM 94
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 261 YTSGSTGKPKGVLHTTGGYLVYVASTFKEVFDLKQDDVYWCT----ADVGWITGHSYLIY--GPLANGTT-TL------- 326
Cdd:cd17639 95 YTSGSTGNPKGVMLTHGNLVAGIAGLGDRVPELLGPDDRYLAylplAHIFELAAENVCLYrgGTIGYGSPrTLtdkskrg 174
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 327 -----------MFEGVPQypTWARLGH-VVDK------HKVSILYTAPTAIRAMMREGD----------SYVRESNRSSL 378
Cdd:cd17639 175 ckgdltefkptLMVGVPA--IWDTIRKgVLAKlnpmggLKRTLFWTAYQSKLKALKEGPgtplldelvfKKVRAALGGRL 252
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 379 RLLGSVGEPINPEAWNWyYNVVGegrCPIVDTWWQTETGGILIAPLPGatALKPGSATRPLFGIQPAIVD-GEGNELEGA 457
Cdd:cd17639 253 RYMLSGGAPLSADTQEF-LNIVL---CPVIQGYGLTETCAGGTVQDPG--DLETGRVGPPLPCCEIKLVDwEEGGYSTDK 326
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 458 AE--GNLVIKdswpGQMRT--IWGDPDRFIEAYFSTfknTYF-TGDGARRDEDGYYWITGRVDD-VLNVSGHRLGTAEIE 531
Cdd:cd17639 327 PPprGEILIR----GPNVFkgYYKNPEKTKEAFDGD---GWFhTGDIGEFHPDGTLKIIDRKKDlVKLQNGEYIALEKLE 399
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 446925704 532 SALVSHEAVAEAAVVGMPHDIKgqgICTFVtlqagVPESEELRK 575
Cdd:cd17639 400 SIYRSNPLVNNICVYADPDKSY---PVAIV-----VPNEKHLTK 435
|
|
| PRK07768 |
PRK07768 |
long-chain-fatty-acid--CoA ligase; Validated |
96-611 |
2.41e-10 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236091 [Multi-domain] Cd Length: 545 Bit Score: 63.48 E-value: 2.41e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 96 EGDHPSRHkivSYKELHDEVCRFANVLKKYGIGKGDRV-VLYMPMVTEAAIAMLACARIGAVHCVvfggFSPDS---LAS 171
Cdd:PRK07768 23 EPDAPVRH---TWGEVHERARRIAGGLAAAGVGPGDAVaVLAGAPVEIAPTAQGLWMRGASLTML----HQPTPrtdLAV 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 172 RIEDS-------QAKLVITADSSLRAGKLLplkenvdlalalpgtecVENVIVVYRNANPIEMKPGRdlwyhliimevda 244
Cdd:PRK07768 96 WAEDTlrvigmiGAKAVVVGEPFLAAAPVL-----------------EEKGIRVLTVADLLAADPID------------- 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 245 ncpPEPMKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVASTFKEV-FDLKQD-DVYW--CTADVGWItghSYLIYgPLA 320
Cdd:PRK07768 146 ---PVETGEDDLALMQLTSGSTGSPKAVQITHGNLYANAEAMFVAAeFDVETDvMVSWlpLFHDMGMV---GFLTV-PMY 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 321 NG-----TTTLMFEGVPQypTWARLghvVDKHKVSILyTAPTAIRAM----MREGDSYvRESNRSSLRLLGSVGEPINPE 391
Cdd:PRK07768 219 FGaelvkVTPMDFLRDPL--LWAEL---ISKYRGTMT-AAPNFAYALlarrLRRQAKP-GAFDLSSLRFALNGAEPIDPA 291
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 392 AWNWYYNV----------------------------VGEGrcPIVDtwwqtETGGILIAPLPGATALKPGSATR------ 437
Cdd:PRK07768 292 DVEDLLDAgarfglrpeailpaygmaeatlavsfspCGAG--LVVD-----EVDADLLAALRRAVPATKGNTRRlatlgp 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 438 PLFGIQPAIVDGEGNELEGAAEGNLVIK-DSWPGQMRTiwgdPDRFIEAyfSTFKNTYFTGDGARRDEDGYYWITGRVDD 516
Cdd:PRK07768 365 PLPGLEVRVVDEDGQVLPPRGVGVIELRgESVTPGYLT----MDGFIPA--QDADGWLDTGDLGYLTEEGEVVVCGRVKD 438
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 517 VLNVSGHRLGTAEIESALVSHEAV--AEAAVVGMPHDIKGQGICTFVTLQAGVPESE--ELRKELISWVRKVLGpvASPD 592
Cdd:PRK07768 439 VIIMAGRNIYPTDIERAAARVEGVrpGNAVAVRLDAGHSREGFAVAVESNAFEDPAEvrRIRHQVAHEVVAEVG--VRPR 516
|
570 580
....*....|....*....|.
gi 446925704 593 ALHWAPA--LPKTRSGKIMRR 611
Cdd:PRK07768 517 NVVVLGPgsIPKTPSGKLRRA 537
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
77-623 |
4.44e-10 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 63.26 E-value: 4.44e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 77 ANCLDRHLKEHPHKPAIIWEGDHPSRHKIVSYKELHDEVCRFANVLKKYGiGKGDRVVLYMPMVTEAAIAMLACARIGAV 156
Cdd:PRK05691 12 VQALQRRAAQTPDRLALRFLADDPGEGVVLSYRDLDLRARTIAAALQARA-SFGDRAVLLFPSGPDYVAAFFGCLYAGVI 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 157 hcvVFGGFSPDS--------LASRIEDSQAKLVITaDSSLRaGKLLPLKENvdLALALPGTECVENVIVVYRNAnpiemk 228
Cdd:PRK05691 91 ---AVPAYPPESarrhhqerLLSIIADAEPRLLLT-VADLR-DSLLQMEEL--AAANAPELLCVDTLDPALAEA------ 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 229 pgrdlWYhliimevdancppEP-MKAEDPLFILYTSGSTGKPKGVlHTTGGYLVYVASTFKEVF--DLKQDDVYwctadV 305
Cdd:PRK05691 158 -----WQ-------------EPaLQPDDIAFLQYTSGSTALPKGV-QVSHGNLVANEQLIRHGFgiDLNPDDVI-----V 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 306 GWIT-GHSYLIYGPLANGtttlMFEGVP---QYPTW-----ARLGHVVDKHKVSILYTAPTAIR-AMMREGDSYVRESNR 375
Cdd:PRK05691 214 SWLPlYHDMGLIGGLLQP----IFSGVPcvlMSPAYflerpLRWLEAISEYGGTISGGPDFAYRlCSERVSESALERLDL 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 376 SSLRLLGSVGEPI-----------------NPEAWNWYYN-------VVGEGRcpivdtwwqtetgGILIAPLP-GATAL 430
Cdd:PRK05691 290 SRWRVAYSGSEPIrqdslerfaekfaacgfDPDSFFASYGlaeatlfVSGGRR-------------GQGIPALElDAEAL 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 431 -----KPGSATrPLFGI---QP--AIVDGEGNELEGAAEGNL-VIKDSWPGQMRTIWGDPdrfiEAYFSTFKN----TYF 495
Cdd:PRK05691 357 arnraEPGTGS-VLMSCgrsQPghAVLIVDPQSLEVLGDNRVgEIWASGPSIAHGYWRNP----EASAKTFVEhdgrTWL 431
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 496 -TGD-GARRDedGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVA---EAAVVGMPHD-IKGQGICTFVT--LQAGV 567
Cdd:PRK05691 432 rTGDlGFLRD--GELFVTGRLKDMLIVRGHNLYPQDIEKTVEREVEVVrkgRVAAFAVNHQgEEGIGIAAEISrsVQKIL 509
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446925704 568 PEseelrKELISWVRKVlgpVAspDALHWAP---------ALPKTRSGKIMRRILR-KIAANELDS 623
Cdd:PRK05691 510 PP-----QALIKSIRQA---VA--EACQEAPsvvlllnpgALPKTSSGKLQRSACRlRLADGSLDS 565
|
|
| LC_FACS_bac1 |
cd17641 |
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ... |
104-613 |
6.33e-10 |
|
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341296 [Multi-domain] Cd Length: 569 Bit Score: 62.06 E-value: 6.33e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 104 KIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVIT 183
Cdd:cd17641 10 QEFTWADYADRVRAFALGLLALGVGRGDVVAILGDNRPEWVWAELAAQAIGALSLGIYQDSMAEEVAYLLNYTGARVVIA 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 184 ADsslragkllplKENVDLALAL-PGTECVENVIVVyrnaNPIEMKPGRD---LWYHLIIMEVDANCPPEP--------- 250
Cdd:cd17641 90 ED-----------EEQVDKLLEIaDRIPSVRYVIYC----DPRGMRKYDDprlISFEDVVALGRALDRRDPglyerevaa 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 251 MKAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVAsTFKEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANG-------- 322
Cdd:cd17641 155 GKGEDVAVLCTTSGTTGKPKLAMLSHGNFLGHCA-AYLAADPLGPGDEYVSVLPLPWIGEQMYSVGQALVCGfivnfpee 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 323 TTTLM-----------------FEGVpQYPTWAR------LGHVVDKHKVSILYTA----------PTAIRAMMREGDSY 369
Cdd:cd17641 234 PETMMedlreigptfvllpprvWEGI-AADVRARmmdatpFKRFMFELGMKLGLRAldrgkrgrpvSLWLRLASWLADAL 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 370 VRESNR-----SSLRLLGSVGEPINPEAWNwYYNVVGegrCPIVDTWWQTETGGILIAPLPGatALKPGSATRPLFGIQP 444
Cdd:cd17641 313 LFRPLRdrlgfSRLRSAATGGAALGPDTFR-FFHAIG---VPLKQLYGQTELAGAYTVHRDG--DVDPDTVGVPFPGTEV 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 445 AIvdgegnelegAAEGNLVIKDswPGQMRTIWGDPdrfiEAYFSTFKNTYF--TGDGARRDEDGYYWITGRVDDVLNVS- 521
Cdd:cd17641 387 RI----------DEVGEILVRS--PGVFVGYYKNP----EATAEDFDEDGWlhTGDAGYFKENGHLVVIDRAKDVGTTSd 450
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 522 GHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGIC---------------TFVTLQ--AGVPESEEL-RKEliswVRK 583
Cdd:cd17641 451 GTRFSPQFIENKLKFSPYIAEAVVLGAGRPYLTAFICidyaivgkwaeqrgiAFTTYTdlASRPEVYELiRKE----VEK 526
|
570 580 590
....*....|....*....|....*....|....*....
gi 446925704 584 VLGPVASPDALHWAPALPK---------TRSGKIMRRIL 613
Cdd:cd17641 527 VNASLPEAQRIRRFLLLYKeldaddgelTRTRKVRRGVI 565
|
|
| LC-FACS_euk |
cd05927 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ... |
102-329 |
9.17e-10 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.
Pssm-ID: 341250 [Multi-domain] Cd Length: 545 Bit Score: 61.46 E-value: 9.17e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 102 RHKIVSYKELHDEVCRFANVLKKYGI--GKGDRVVLYMPMVTEAAIAMLACARIGAVhCVVF----GgfsPDSLASRIED 175
Cdd:cd05927 2 PYEWISYKEVAERADNIGSALRSLGGkpAPASFVGIYSINRPEWIISELACYAYSLV-TVPLydtlG---PEAIEYILNH 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 176 SQAKLVItadsslragkllplkenvdlalalpgteCVENVIVVyrnanpiemkpgrdLWYHLIIMEVDANCPPEPMKAED 255
Cdd:cd05927 78 AEISIVF----------------------------CDAGVKVY--------------SLEEFEKLGKKNKVPPPPPKPED 115
|
170 180 190 200 210 220 230
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 446925704 256 PLFILYTSGSTGKPKGVLHTTGGYLVYVASTFKEVFDLK---QDDVYWctadvgwitghSYLiygPLANgtttlMFE 329
Cdd:cd05927 116 LATICYTSGTTGNPKGVMLTHGNIVSNVAGVFKILEILNkinPTDVYI-----------SYL---PLAH-----IFE 173
|
|
| PLN02430 |
PLN02430 |
long-chain-fatty-acid-CoA ligase |
107-299 |
2.58e-09 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178049 [Multi-domain] Cd Length: 660 Bit Score: 60.21 E-value: 2.58e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 107 SYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVhCV-VFGGFSPDSLASRIEDSQAKLVITAD 185
Cdd:PLN02430 78 TYKEVYEEVLQIGSALRASGAEPGSRVGIYGSNCPQWIVAMEACAAHSLI-CVpLYDTLGPGAVDYIVDHAEIDFVFVQD 156
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 186 SSLRAgkllPLKENVDLALALPGTECVENVIVVYRN-ANPIEMKPGRdlWYHLIIMEVDANCPPEPMKAEDPLFILYTSG 264
Cdd:PLN02430 157 KKIKE----LLEPDCKSAKRLKAIVSFTSVTEEESDkASQIGVKTYS--WIDFLHMGKENPSETNPPKPLDICTIMYTSG 230
|
170 180 190
....*....|....*....|....*....|....*....
gi 446925704 265 STGKPKGVLHTTGGYLVYVAST--FKEVFDLKQ--DDVY 299
Cdd:PLN02430 231 TSGDPKGVVLTHEAVATFVRGVdlFMEQFEDKMthDDVY 269
|
|
| PTZ00216 |
PTZ00216 |
acyl-CoA synthetase; Provisional |
95-277 |
1.08e-08 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240316 [Multi-domain] Cd Length: 700 Bit Score: 58.45 E-value: 1.08e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 95 WEGDHPSRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIE 174
Cdd:PTZ00216 111 MEVTHFNETRYITYAELWERIVNFGRGLAELGLTKGSNVAIYEETRWEWLASIYGIWSQSMVAATVYANLGEDALAYALR 190
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 175 DSQAKLVITADSSLraGKLLPLKENVdlalALPGTecvenvIVVYRNANPiemkPGRDL-------WYHLIIM---EVDA 244
Cdd:PTZ00216 191 ETECKAIVCNGKNV--PNLLRLMKSG----GMPNT------TIIYLDSLP----ASVDTegcrlvaWTDVVAKghsAGSH 254
|
170 180 190
....*....|....*....|....*....|...
gi 446925704 245 NCPPEPMKAEDPLFILYTSGSTGKPKGVLHTTG 277
Cdd:PTZ00216 255 HPLNIPENNDDLALIMYTSGTTGDPKGVMHTHG 287
|
|
| PRK06814 |
PRK06814 |
acyl-[ACP]--phospholipid O-acyltransferase; |
126-618 |
3.32e-08 |
|
acyl-[ACP]--phospholipid O-acyltransferase;
Pssm-ID: 235865 [Multi-domain] Cd Length: 1140 Bit Score: 56.90 E-value: 3.32e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 126 GIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVfgGFS--PDSLASRIEDSQAKLVITADSSLRAGKLLPLKENVDLA 203
Cdd:PRK06814 678 NTPPGENVGVMLPNANGAAVTFFALQSAGRVPAMI--NFSagIANILSACKAAQVKTVLTSRAFIEKARLGPLIEALEFG 755
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 204 LALPGTECVENVIvvyRNANPIE--MKPGRDLWYHliimevdanCPPEPmkaEDPLFILYTSGSTGKPKGVLHTTGGYLV 281
Cdd:PRK06814 756 IRIIYLEDVRAQI---GLADKIKglLAGRFPLVYF---------CNRDP---DDPAVILFTSGSEGTPKGVVLSHRNLLA 820
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 282 YVASTFKEVfDLKQDDVYWCTADVgwitGHSYLIYG----PLANGTTTLMFEG------VPQ--YPTWArlghvvdkhkv 349
Cdd:PRK06814 821 NRAQVAARI-DFSPEDKVFNALPV----FHSFGLTGglvlPLLSGVKVFLYPSplhyriIPEliYDTNA----------- 884
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 350 SILYTAPTAIRAMMREGDSYvresNRSSLRLLGSVGEPINPEAWNWYYNVVGegrCPIVDTWWQTETGGILIAPLPgaTA 429
Cdd:PRK06814 885 TILFGTDTFLNGYARYAHPY----DFRSLRYVFAGAEKVKEETRQTWMEKFG---IRILEGYGVTETAPVIALNTP--MH 955
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 430 LKPGSATRPLFGIQPAIVDGEGNElEGaaeGNLVIKDswP----GQMRTiwgDPDRFIEAYFSTFkntYFTGDGARRDED 505
Cdd:PRK06814 956 NKAGTVGRLLPGIEYRLEPVPGID-EG---GRLFVRG--PnvmlGYLRA---ENPGVLEPPADGW---YDTGDIVTIDEE 1023
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 506 GYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTlqagvpESEELRKELISWVRKV- 584
Cdd:PRK06814 1024 GFITIKGRAKRFAKIAGEMISLAAVEELAAELWPDALHAAVSIPDARKGERIILLTT------ASDATRAAFLAHAKAAg 1097
|
490 500 510
....*....|....*....|....*....|....*...
gi 446925704 585 LGPVASPDALHWAPALPKTRSGKI----MRRILRKIAA 618
Cdd:PRK06814 1098 ASELMVPAEIITIDEIPLLGTGKIdyvaVTKLAEEAAA 1135
|
|
| PLN02861 |
PLN02861 |
long-chain-fatty-acid-CoA ligase |
106-321 |
3.50e-08 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178452 [Multi-domain] Cd Length: 660 Bit Score: 56.77 E-value: 3.50e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 106 VSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITAD 185
Cdd:PLN02861 78 LTYKEVYDAAIRIGSAIRSRGVNPGDRCGIYGSNCPEWIIAMEACNSQGITYVPLYDTLGANAVEFIINHAEVSIAFVQE 157
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 186 SSLRAgkllplkenvdLALALPGTECVENVIVVYRNANPIEMKPGRDL------WYHLIIM-EVDANCPPEpmKAEDPLF 258
Cdd:PLN02861 158 SKISS-----------ILSCLPKCSSNLKTIVSFGDVSSEQKEEAEELgvscfsWEEFSLMgSLDCELPPK--QKTDICT 224
|
170 180 190 200 210 220
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 446925704 259 ILYTSGSTGKPKGVLHTTGGYLVYVASTFKEvfdLKQDDVYWCTADvgwitghSYLIYGPLAN 321
Cdd:PLN02861 225 IMYTSGTTGEPKGVILTNRAIIAEVLSTDHL---LKVTDRVATEED-------SYFSYLPLAH 277
|
|
| AFD_CAR-like |
cd17632 |
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ... |
102-275 |
4.82e-08 |
|
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.
Pssm-ID: 341287 [Multi-domain] Cd Length: 588 Bit Score: 55.92 E-value: 4.82e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 102 RHKIVSYKELHDEVCRFANVLK-KYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKL 180
Cdd:cd17632 64 RFETITYAELWERVGAVAAAHDpEQPVRPGDFVAVLGFTSPDYATVDLALTRLGAVSVPLQAGASAAQLAPILAETEPRL 143
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 181 vITADSslragkllplkENVDLALALPGTECVENVIVVYRNANPIE-------------MKPGRDLWYHLIIMEVDANCP 247
Cdd:cd17632 144 -LAVSA-----------EHLDLAVEAVLEGGTPPRLVVFDHRPEVDahraalesarerlAAVGIPVTTLTLIAVRGRDLP 211
|
170 180 190
....*....|....*....|....*....|...
gi 446925704 248 PEPMKAE----DPL-FILYTSGSTGKPKGVLHT 275
Cdd:cd17632 212 PAPLFRPepddDPLaLLIYTSGSTGTPKGAMYT 244
|
|
| FATP_FACS |
cd05940 |
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ... |
107-615 |
6.47e-08 |
|
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341263 [Multi-domain] Cd Length: 449 Bit Score: 55.44 E-value: 6.47e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 107 SYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDSLASRIEDSQAKLVITads 186
Cdd:cd05940 5 TYAELDAMANRYARWLKSLGLKPGDVVALFMENRPEYVLLWLGLVKIGAVAALINYNLRGESLAHCLNVSSAKHLVV--- 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 187 slragkllplkenvdlalalpgtecvenvivvyrnanpiemkpgrdlwyhliimevdancppepmkaeDPLFILYTSGST 266
Cdd:cd05940 82 --------------------------------------------------------------------DAALYIYTSGTT 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 267 GKPKGVLHTTGGYLVYVASTFKEVFDLKQDDVYWCTAdvgwiTGHSY-LIYGP---LANGTTTLMFEGVPQYPTWARlgh 342
Cdd:cd05940 94 GLPKAAIISHRRAWRGGAFFAGSGGALPSDVLYTCLP-----LYHSTaLIVGWsacLASGATLVIRKKFSASNFWDD--- 165
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 343 vVDKHKVSILYTAPTAIRAMMREGDSyvRESNRSSLRLLgsVGEPINPEAWNWYYNVVGEGRcpIVDTWWQTE--TGGIL 420
Cdd:cd05940 166 -IRKYQATIFQYIGELCRYLLNQPPK--PTERKHKVRMI--FGNGLRPDIWEEFKERFGVPR--IAEFYAATEgnSGFIN 238
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 421 IAPLPGATALKPgSATRPLFGIQPAIVDGEGNELEGAAEGnLVIK--DSWPGQMRTIWGDPDRFiEAYFSTFKNT----- 493
Cdd:cd05940 239 FFGKPGAIGRNP-SLLRKVAPLALVKYDLESGEPIRDAEG-RCIKvpRGEPGLLISRINPLEPF-DGYTDPAATEkkilr 315
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 494 --------YF-TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMP---HDikGQGICTFV 561
Cdd:cd05940 316 dvfkkgdaWFnTGDLMRLDGEGFWYFVDRLGDTFRWKGENVSTTEVAAVLGAFPGVEEANVYGVQvpgTD--GRAGMAAI 393
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 446925704 562 TLQAGvpESEELRKeLISWVRKVLGPVASPDALHWAPALPKTRSGKIMRRILRK 615
Cdd:cd05940 394 VLQPN--EEFDLSA-LAAHLEKNLPGYARPLFLRLQPEMEITGTFKQQKVDLRN 444
|
|
| PRK04813 |
PRK04813 |
D-alanine--poly(phosphoribitol) ligase subunit DltA; |
80-613 |
5.32e-07 |
|
D-alanine--poly(phosphoribitol) ligase subunit DltA;
Pssm-ID: 235313 [Multi-domain] Cd Length: 503 Bit Score: 52.59 E-value: 5.32e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 80 LDRHLKEHPHKPAIIWEGDhpsRHkivSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIG----- 154
Cdd:PRK04813 8 IEEFAQTQPDFPAYDYLGE---KL---TYGQLKEDSDALAAFIDSLKLPDKSPIIVFGHMSPEMLATFLGAVKAGhayip 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 155 -AVHcvvfggfSP-DSLASRIEDSQAKLVITADsslragkllplkenvDLALALPGTECV--ENVIVVYRNANPIEMKpg 230
Cdd:PRK04813 82 vDVS-------SPaERIEMIIEVAKPSLIIATE---------------ELPLEILGIPVItlDELKDIFATGNPYDFD-- 137
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 231 rdlwyhliimevdancppEPMKAEDPLFILYTSGSTGKPKGV--LHTTggyLVyvasTFKEvfdlkqddvyWCTADVGWI 308
Cdd:PRK04813 138 ------------------HAVKGDDNYYIIFTSGTTGKPKGVqiSHDN---LV----SFTN----------WMLEDFALP 182
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 309 TGHSYL-------------IYGPLANGTT------------TLMFEGVPQYP--TWarlghvvdkhkVSilyTAPTAIRA 361
Cdd:PRK04813 183 EGPQFLnqapysfdlsvmdLYPTLASGGTlvalpkdmtanfKQLFETLPQLPinVW-----------VS---TPSFADMC 248
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 362 MMregDSYVRESNRSSLRLLGSVGEPI-NPEAWNWYynvvgeGRCP---IVDTWWQTETGGILIA------------PLP 425
Cdd:PRK04813 249 LL---DPSFNEEHLPNLTHFLFCGEELpHKTAKKLL------ERFPsatIYNTYGPTEATVAVTSieitdemldqykRLP 319
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 426 -GATalKPGSatrPLFgiqpaIVDGEGNELEGAAEGNLVIkdSWPGQMRTIWGDPDRFIEAYFsTFKN--TYFTGDGARR 502
Cdd:PRK04813 320 iGYA--KPDS---PLL-----IIDEEGTKLPDGEQGEIVI--SGPSVSKGYLNNPEKTAEAFF-TFDGqpAYHTGDAGYL 386
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 503 DeDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESE-ELRKELISWV 581
Cdd:PRK04813 387 E-DGLLFYQGRIDFQIKLNGYRIELEEIEQNLRQSSYVESAVVVPYNKDHKVQYLIAYVVPKEEDFEREfELTKAIKKEL 465
|
570 580 590
....*....|....*....|....*....|..
gi 446925704 582 RKVLGPVASPDALHWAPALPKTRSGKIMRRIL 613
Cdd:PRK04813 466 KERLMEYMIPRKFIYRDSLPLTPNGKIDRKAL 497
|
|
| PRK08043 |
PRK08043 |
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase; |
107-620 |
6.13e-07 |
|
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;
Pssm-ID: 181207 [Multi-domain] Cd Length: 718 Bit Score: 52.79 E-value: 6.13e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 107 SYKELHDEVCRFANVLKKYGiGKGDRVVLYMPMVTEAAIAMLACA---RIGAVHCVVFGGfspDSLASRIEDSQAKLVIT 183
Cdd:PRK08043 233 SYRKLLKKTLFVGRILEKYS-VEGERIGLMLPNATISAAVIFGASlrrRIPAMMNYTAGV---KGLTSAITAAEIKTIFT 308
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 184 ADSSLRAGKLLPLKENVDlalalpgtecveNVIVVYRNANPIEMKPGRDLW--YHLIiMEVDANCPPEPmkaEDPLFILY 261
Cdd:PRK08043 309 SRQFLDKGKLWHLPEQLT------------QVRWVYLEDLKDDVTTADKLWifAHLL-MPRLAQVKQQP---EDAALILF 372
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 262 TSGSTGKPKGVLHTTGGYLVYVaSTFKEVFDLKQDDVYWCTADVgwitGHSY-LIYGPLangtTTLMfegvpqypTWARL 340
Cdd:PRK08043 373 TSGSEGHPKGVVHSHKSLLANV-EQIKTIADFTPNDRFMSALPL----FHSFgLTVGLF----TPLL--------TGAEV 435
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 341 ghvvdkhkvsILYTAPTAIRaMMREgdsYVRESNRSSL----RLLGSVGEPINPeaWNWY---YNVVG-EGRCPIVDTWW 412
Cdd:PRK08043 436 ----------FLYPSPLHYR-IVPE---LVYDRNCTVLfgtsTFLGNYARFANP--YDFArlrYVVAGaEKLQESTKQLW 499
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 413 QtETGGILIAPLPGAT------------ALKPGSATRPLFGIQ------PAIVDGEGNELEGA--AEGNLVIKDswPGQM 472
Cdd:PRK08043 500 Q-DKFGLRILEGYGVTecapvvsinvpmAAKPGTVGRILPGMDarllsvPGIEQGGRLQLKGPniMNGYLRVEK--PGVL 576
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 473 rtiwgDPDRFIEAYFSTFKNTYFTGDGARRDEDGYYWITGRVDDVLNVSGH--RLGTAEIESALVSHEAVAEAAVVgmPH 550
Cdd:PRK08043 577 -----EVPTAENARGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEmvSLEMVEQLALGVSPDKQHATAIK--SD 649
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 446925704 551 DIKGQGICTFVTlqagvpESEELRKELISWVRKVLGP-VASPDALHWAPALPKTRSGKIMRRILRKIAANE 620
Cdd:PRK08043 650 ASKGEALVLFTT------DSELTREKLQQYAREHGVPeLAVPRDIRYLKQLPLLGSGKPDFVTLKSMVDEP 714
|
|
| PRK09188 |
PRK09188 |
serine/threonine protein kinase; Provisional |
530-626 |
1.96e-06 |
|
serine/threonine protein kinase; Provisional
Pssm-ID: 236400 [Multi-domain] Cd Length: 365 Bit Score: 50.53 E-value: 1.96e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 530 IESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPEsEELRKELiswvrKVLGPVASPDALHWAPALPKTRSGKIM 609
Cdd:PRK09188 245 IQAALKSDPAVSDVAIALFSLPAKGVGLYAFVEAELPADE-KSLRARL-----AGAKPPKPPEHIQPVAALPRDADGTVR 318
|
90
....*....|....*..
gi 446925704 610 RRILRKIAANELDSLGD 626
Cdd:PRK09188 319 DDILRLIAMNQIDELDD 335
|
|
| A_NRPS_MycA_like |
cd05908 |
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ... |
253-615 |
4.23e-06 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341234 [Multi-domain] Cd Length: 499 Bit Score: 49.79 E-value: 4.23e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 253 AEDPLFILYTSGSTGKPKGVLHTTGGyLVYVASTFKEVFDLKQDDVY--W--CTADVGWITGH-SYLIYGPLANGTTTLM 327
Cdd:cd05908 105 ADELAFIQFSSGSTGDPKGVMLTHEN-LVHNMFAILNSTEWKTKDRIlsWmpLTHDMGLIAFHlAPLIAGMNQYLMPTRL 183
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 328 FEGVPQypTWARlghVVDKHKVSILYTAPTAIRAMMRE-GDSYVRESNRSSLRLLGSVGEPINPEAW------------- 393
Cdd:cd05908 184 FIRRPI--LWLK---KASEHKATIVSSPNFGYKYFLKTlKPEKANDWDLSSIRMILNGAEPIDYELChefldhmskyglk 258
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 394 -NWYYNVVG-----EGRC----------PIVDTWWQTETGGILIAPLPGATALKPGSATRPLFGIQPAIVDGEGNELEGA 457
Cdd:cd05908 259 rNAILPVYGlaeasVGASlpkaqspfktITLGRRHVTHGEPEPEVDKKDSECLTFVEVGKPIDETDIRICDEDNKILPDG 338
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 458 AEGNLVIKdswpGQMRTiwgdpdrfiEAYFSTFKNT--YFTGDGARRDED------GYYWITGRVDDVLNVSGHRLGTAE 529
Cdd:cd05908 339 YIGHIQIR----GKNVT---------PGYYNNPEATakVFTDDGWLKTGDlgfirnGRLVITGREKDIIFVNGQNVYPHD 405
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 530 IESALVSHEAV--AEAAVVGMpHD--IKGQGICTFVTLQAGVPESEELRKELISWVRKVLG-------PVAspdalhwap 598
Cdd:cd05908 406 IERIAEELEGVelGRVVACGV-NNsnTRNEEIFCFIEHRKSEDDFYPLGKKIKKHLNKRGGwqinevlPIR--------- 475
|
410
....*....|....*..
gi 446925704 599 ALPKTRSGKIMRRILRK 615
Cdd:cd05908 476 RIPKTTSGKVKRYELAQ 492
|
|
| PRK07868 |
PRK07868 |
acyl-CoA synthetase; Validated |
117-605 |
5.94e-06 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236121 [Multi-domain] Cd Length: 994 Bit Score: 49.72 E-value: 5.94e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 117 RFANVLK---KYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVfggfSPDS-LASRIEDSQAKLVITADSSLRAGK 192
Cdd:PRK07868 481 RINNVVRgliAVGVRQGDRVGVLMETRPSALVAIAALSRLGAVAVLM----PPDTdLAAAVRLGGVTEIITDPTNLEAAR 556
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 193 LLPLKenvdlALALPGTEcvENVIVVYRNANPIEMK---------PGrdlWYHliimevdancpPEPMKAEDPLFILYTs 263
Cdd:PRK07868 557 QLPGR-----VLVLGGGE--SRDLDLPDDADVIDMEkidpdavelPG---WYR-----------PNPGLARDLAFIAFS- 614
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 264 gstgkpkgvlhTTGGYLV--------YVASTF--KEVFDLKQDDVYWCTADVGWITGHSYLIYGPLANGTTTLMFEGV-P 332
Cdd:PRK07868 615 -----------TAGGELVakqitnyrWALSAFgtASAAALDRRDTVYCLTPLHHESGLLVSLGGAVVGGSRIALSRGLdP 683
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 333 qyptwARLGHVVDKHKVSIL-YTAptairAMMRE--GDSYVRESNRSSLRLLGSVGEPInpEAWNWYYNVVGEGRcpiVD 409
Cdd:PRK07868 684 -----DRFVQEVRQYGVTVVsYTW-----AMLREvvDDPAFVLHGNHPVRLFIGSGMPT--GLWERVVEAFAPAH---VV 748
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 410 TWWQTETGGILIAPLPGAtalKPGSATRPLFGiqpaivdgeGNELEGAA---EGNLVIKD-------SWPGQMRTIWGDP 479
Cdd:PRK07868 749 EFFATTDGQAVLANVSGA---KIGSKGRPLPG---------AGRVELAAydpEHDLILEDdrgfvrrAEVNEVGVLLARA 816
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 480 DRFIEAYFSTFKNTYFTGDG-------ARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDI 552
Cdd:PRK07868 817 RGPIDPTASVKRGVFAPADTwisteylFRRDDDGDYWLVDRRGSVIRTARGPVYTEPVTDALGRIGGVDLAVTYGVEVGG 896
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 446925704 553 KGQGICTFVTLQAGVPESEELRKELISwvrkvLGPVASPDALHWAPALPKTRS 605
Cdd:PRK07868 897 RQLAVAAVTLRPGAAITAADLTEALAS-----LPVGLGPDIVHVVPEIPLSAT 944
|
|
| PLN02736 |
PLN02736 |
long-chain acyl-CoA synthetase |
100-320 |
9.35e-06 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 178337 [Multi-domain] Cd Length: 651 Bit Score: 48.94 E-value: 9.35e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 100 PSRHKIVSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVFGGFSPDslasriedsqAK 179
Cdd:PLN02736 73 VGEYKWMTYGEAGTARTAIGSGLVQHGIPKGACVGLYFINRPEWLIVDHACSAYSYVSVPLYDTLGPD----------AV 142
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 180 LVITADSSLRAGKLLPLKENVDLALaLPGTECVENVIVVYRNANPIEMKPG----RDLWYHLIIMEVDANC-PPEPMKAE 254
Cdd:PLN02736 143 KFIVNHAEVAAIFCVPQTLNTLLSC-LSEIPSVRLIVVVGGADEPLPSLPSgtgvEIVTYSKLLAQGRSSPqPFRPPKPE 221
|
170 180 190 200 210 220
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446925704 255 DPLFILYTSGSTGKPKGVLHTTGGYLVYVASTfkevfdlkQDDVYWCTADVgwitghsYLIYGPLA 320
Cdd:PLN02736 222 DVATICYTSGTTGTPKGVVLTHGNLIANVAGS--------SLSTKFYPSDV-------HISYLPLA 272
|
|
| PRK08180 |
PRK08180 |
feruloyl-CoA synthase; Reviewed |
106-275 |
2.25e-05 |
|
feruloyl-CoA synthase; Reviewed
Pssm-ID: 236175 [Multi-domain] Cd Length: 614 Bit Score: 47.57 E-value: 2.25e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 106 VSYKELHDEVCRFANVLKKYGIGKGDRVVLYMPMVTEAAIAMLACARIGAVHCVVfggfSPD-SLASR--------IEDS 176
Cdd:PRK08180 70 LTYAEALERVRAIAQALLDRGLSAERPLMILSGNSIEHALLALAAMYAGVPYAPV----SPAySLVSQdfgklrhvLELL 145
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 177 QAKLVITADSSLRAGkllplkenvdlALALPGTECVEnvIVVYRNAnpiemKPGRDLwyHLIIMEVDANCPPEPMKA--- 253
Cdd:PRK08180 146 TPGLVFADDGAAFAR-----------ALAAVVPADVE--VVAVRGA-----VPGRAA--TPFAALLATPPTAAVDAAhaa 205
|
170 180
....*....|....*....|....*
gi 446925704 254 ---EDPLFILYTSGSTGKPKGVLHT 275
Cdd:PRK08180 206 vgpDTIAKFLFTSGSTGLPKAVINT 230
|
|
| hsFATP2a_ACSVL_like |
cd05938 |
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar ... |
105-302 |
8.35e-04 |
|
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar proteins; Fatty acid transport proteins (FATP) of this family transport long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes hsFATP2, hsFATP5, and hsFATP6, and similar proteins. Each FATP has unique patterns of tissue distribution. These FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. The hsFATP proteins exist in two splice variants; the b variant, lacking exon 3, has no acyl-CoA synthetase activity. FATPs are key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341261 [Multi-domain] Cd Length: 537 Bit Score: 42.28 E-value: 8.35e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 105 IVSYKELHDEVCRFANVLKKY-GIGKGDRVVLYMPMVTEAAIAMLACARIG-AVHCVVFGGFSpDSLASRIEDSQAKlVI 182
Cdd:cd05938 5 TYTYRDVDRRSNQAARALLAHaGLRPGDTVALLLGNEPAFLWIWLGLAKLGcPVAFLNTNIRS-KSLLHCFRCCGAK-VL 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 183 TADSSLRAG--KLLP--LKENVDLALALPGTECvENVIVVYR--NANPIEMKPGrDLWYHLIIMevdancppepmkaeDP 256
Cdd:cd05938 83 VVAPELQEAveEVLPalRADGVSVWYLSHTSNT-EGVISLLDkvDAASDEPVPA-SLRAHVTIK--------------SP 146
|
170 180 190 200
....*....|....*....|....*....|....*....|....*.
gi 446925704 257 LFILYTSGSTGKPKGVLHTTggYLVYVASTFKEVFDLKQDDVYWCT 302
Cdd:cd05938 147 ALYIYTSGTTGLPKAARISH--LRVLQCSGFLSLCGVTADDVIYIT 190
|
|
| PRK05851 |
PRK05851 |
long-chain-fatty acid--ACP ligase MbtM; |
505-610 |
2.73e-03 |
|
long-chain-fatty acid--ACP ligase MbtM;
Pssm-ID: 180289 [Multi-domain] Cd Length: 525 Bit Score: 40.90 E-value: 2.73e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446925704 505 DGYYWITGRVDDVLNVSGHRLGTAEIESALVSHEAVAEAAVVGMPHDIKGQGICTFVTLQAGVPESEELRKELISWVRKV 584
Cdd:PRK05851 408 DGGLVVCGRAKELITVAGRNIFPTEIERVAAQVRGVREGAVVAVGTGEGSARPGLVIAAEFRGPDEAGARSEVVQRVASE 487
|
90 100
....*....|....*....|....*..
gi 446925704 585 LGPVASpDALHWAP-ALPKTRSGKIMR 610
Cdd:PRK05851 488 CGVVPS-DVVFVAPgSLPRTSSGKLRR 513
|
|
| |
