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Conserved domains on  [gi|446048548|ref|WP_000126403|]
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MULTISPECIES: AbrB/MazE/SpoVT family DNA-binding domain-containing protein [Bacteria]

Protein Classification

AbrB/MazE/SpoVT family DNA-binding domain-containing protein( domain architecture ID 1230)

AbrB/MazE/SpoVT family DNA-binding domain-containing protein similar to Escherichia coli antitoxin PemI and antitoxin MazE

CATH:  2.10.260.10
Gene Ontology:  GO:0003677
SCOP:  4000397

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MazE_antitoxin super family cl00877
Antidote-toxin recognition MazE, bacterial antitoxin; AbrB-like is a family of small proteins ...
 15-92 4.85e-11

Antidote-toxin recognition MazE, bacterial antitoxin; AbrB-like is a family of small proteins that operate in conjunction with a cognate toxin molecule. The commonly attributed role of toxin-antitoxin systems is to maintain low-copy number plasmids from one generation to the next. Such gene-pairs are also found on chromosomes and to be associated with a number of biological functions such as: reduction of protein synthesis, gene regulation and retardation of cell growth under nutritional stress. This family includes proteins from a number of different pairings, eg MazE, AbrB, VapB, PhoU, PemI-like and SpoVT. MazE is the antidote to the toxin MazF of E. coli. MazE-MazF in E. coli is a regulated prokaryotic chromosomal addiction module. MazE antidote is degraded by the ClpPA protease of the bacterial proteasome. MazE-MazF is thought to play a role in programmed cell death when cells suffer nutrient deprivation, and MazE-MazF modules have also been implicated in the bacteriostatic effects of other addiction modules.


The actual alignment was detected with superfamily member TIGR02609:

Pssm-ID: 469969  Cd Length: 74  Bit Score: 53.54  E-value: 4.85e-11
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446048548  15 RKVTKIGNSLGLTLPQEFYTTLNIKQGDvvSLEINEENEQIVIKKSKKismPEDLNPKFLESLNRTINKFDGAFKDLV 92
Cdd:TIGR02609  1 RKIRKVGNSLVVTLPKEVLESLGLKEGD--TLYVDEEEGGLKLKRFDE---GKELEKKMQMAVERAMSKYDEALKELA 73
 
Name Accession Description Interval E-value
doc_partner TIGR02609
putative addiction module antidote; Members of this protein family are putative addiction ...
 15-92 4.85e-11

putative addiction module antidote; Members of this protein family are putative addiction module antidote proteins that appear recurringly in two-gene operons with members of the Doc (death-on-curing) family TIGR01550. Members of this family contain a SpoVT/AbrB-like domain (pfam04014). Note that the gene pairs with a member of this family tend to be found on bacterial chromosomes, not on plasmids. [Mobile and extrachromosomal element functions, Other]


Pssm-ID: 274230  Cd Length: 74  Bit Score: 53.54  E-value: 4.85e-11
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446048548  15 RKVTKIGNSLGLTLPQEFYTTLNIKQGDvvSLEINEENEQIVIKKSKKismPEDLNPKFLESLNRTINKFDGAFKDLV 92
Cdd:TIGR02609  1 RKIRKVGNSLVVTLPKEVLESLGLKEGD--TLYVDEEEGGLKLKRFDE---GKELEKKMQMAVERAMSKYDEALKELA 73
MazE COG2336
Antitoxin component MazE of the MazEF toxin-antitoxin module [Defense mechanisms];
16-62 5.29e-11

Antitoxin component MazE of the MazEF toxin-antitoxin module [Defense mechanisms];


Pssm-ID: 441907  Cd Length: 80  Bit Score: 53.35  E-value: 5.29e-11
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*..
gi 446048548 16 KVTKIGNSLGLTLPQEFYTTLNIKQGDVVslEINEENEQIVIKKSKK 62
Cdd:COG2336   4 KIRKWGNSLGVRIPKALLEQLGLKEGDEV--ELEVEDGRIVIRPVRR 48
SpoVT_AbrB smart00966
SpoVT / AbrB like domain; This domain is found in AbrB from Bacillus subtilis. The product of ...
 18-62 1.70e-07

SpoVT / AbrB like domain; This domain is found in AbrB from Bacillus subtilis. The product of the abrB gene is an ambiactive repressor and activator of the transcription of genes expressed during the transition state between vegetative growth and the onset of stationary phase and sporulation. AbrB is thought to interact directly with the transcription initiation regions of genes under its control. AbrB contains a helix-turn-helix structure, but this domain ends before the helix-turn-helix begins. The product of the B. subtilis gene spoVT is another member of this family and is also a transcriptional regulator. DNA-binding activity in this AbrB homologue requires hexamerisation. Another family member has been isolated from the Sulfolobus solfataricus and has been identified as a homologue of bacterial repressor-like proteins. The Escherichia coli family member SohA or Prl1F appears to be bifunctional and is able to regulate its own expression as well as relieve the export block imposed by high-level synthesis of beta-galactosidase hybrid proteins.


Pssm-ID: 198034 [Multi-domain]  Cd Length: 45  Bit Score: 43.65  E-value: 1.70e-07
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*
gi 446048548   18 TKIGNSLGLTLPQEFYTTLNIKQGDVVslEINEENEQIVIKKSKK 62
Cdd:smart00966  1 VKVGNSGQVTIPKEVREKLGIKEGDEV--ELEVDGDGIILEPVKK 43
MazE_antitoxin pfam04014
Antidote-toxin recognition MazE, bacterial antitoxin; AbrB-like is a family of small proteins ...
 18-63 1.41e-05

Antidote-toxin recognition MazE, bacterial antitoxin; AbrB-like is a family of small proteins that operate in conjunction with a cognate toxin molecule. The commonly attributed role of toxin-antitoxin systems is to maintain low-copy number plasmids from one generation to the next. Such gene-pairs are also found on chromosomes and to be associated with a number of biological functions such as: reduction of protein synthesis, gene regulation and retardation of cell growth under nutritional stress. This family includes proteins from a number of different pairings, eg MazE, AbrB, VapB, PhoU, PemI-like and SpoVT. MazE is the antidote to the toxin MazF of E. coli. MazE-MazF in E. coli is a regulated prokaryotic chromosomal addiction module. MazE antidote is degraded by the ClpPA protease of the bacterial proteasome. MazE-MazF is thought to play a role in programmed cell death when cells suffer nutrient deprivation, and MazE-MazF modules have also been implicated in the bacteriostatic effects of other addiction modules.


Pssm-ID: 397909 [Multi-domain]  Cd Length: 44  Bit Score: 38.65  E-value: 1.41e-05
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*.
gi 446048548  18 TKIGNSLGLTLPQEFYTTLNIKQGDVVSLEIneENEQIVIKKSKKI 63
Cdd:pfam04014  1 FKVGNSGAVRIPKEVRKALGIKEGDEVELEV--DGDKIILKPKKSE 44
 
Name Accession Description Interval E-value
doc_partner TIGR02609
putative addiction module antidote; Members of this protein family are putative addiction ...
 15-92 4.85e-11

putative addiction module antidote; Members of this protein family are putative addiction module antidote proteins that appear recurringly in two-gene operons with members of the Doc (death-on-curing) family TIGR01550. Members of this family contain a SpoVT/AbrB-like domain (pfam04014). Note that the gene pairs with a member of this family tend to be found on bacterial chromosomes, not on plasmids. [Mobile and extrachromosomal element functions, Other]


Pssm-ID: 274230  Cd Length: 74  Bit Score: 53.54  E-value: 4.85e-11
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446048548  15 RKVTKIGNSLGLTLPQEFYTTLNIKQGDvvSLEINEENEQIVIKKSKKismPEDLNPKFLESLNRTINKFDGAFKDLV 92
Cdd:TIGR02609  1 RKIRKVGNSLVVTLPKEVLESLGLKEGD--TLYVDEEEGGLKLKRFDE---GKELEKKMQMAVERAMSKYDEALKELA 73
MazE COG2336
Antitoxin component MazE of the MazEF toxin-antitoxin module [Defense mechanisms];
16-62 5.29e-11

Antitoxin component MazE of the MazEF toxin-antitoxin module [Defense mechanisms];


Pssm-ID: 441907  Cd Length: 80  Bit Score: 53.35  E-value: 5.29e-11
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*..
gi 446048548 16 KVTKIGNSLGLTLPQEFYTTLNIKQGDVVslEINEENEQIVIKKSKK 62
Cdd:COG2336   4 KIRKWGNSLGVRIPKALLEQLGLKEGDEV--ELEVEDGRIVIRPVRR 48
SpoVT_AbrB smart00966
SpoVT / AbrB like domain; This domain is found in AbrB from Bacillus subtilis. The product of ...
 18-62 1.70e-07

SpoVT / AbrB like domain; This domain is found in AbrB from Bacillus subtilis. The product of the abrB gene is an ambiactive repressor and activator of the transcription of genes expressed during the transition state between vegetative growth and the onset of stationary phase and sporulation. AbrB is thought to interact directly with the transcription initiation regions of genes under its control. AbrB contains a helix-turn-helix structure, but this domain ends before the helix-turn-helix begins. The product of the B. subtilis gene spoVT is another member of this family and is also a transcriptional regulator. DNA-binding activity in this AbrB homologue requires hexamerisation. Another family member has been isolated from the Sulfolobus solfataricus and has been identified as a homologue of bacterial repressor-like proteins. The Escherichia coli family member SohA or Prl1F appears to be bifunctional and is able to regulate its own expression as well as relieve the export block imposed by high-level synthesis of beta-galactosidase hybrid proteins.


Pssm-ID: 198034 [Multi-domain]  Cd Length: 45  Bit Score: 43.65  E-value: 1.70e-07
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*
gi 446048548   18 TKIGNSLGLTLPQEFYTTLNIKQGDVVslEINEENEQIVIKKSKK 62
Cdd:smart00966  1 VKVGNSGQVTIPKEVREKLGIKEGDEV--ELEVDGDGIILEPVKK 43
MazE_antitoxin pfam04014
Antidote-toxin recognition MazE, bacterial antitoxin; AbrB-like is a family of small proteins ...
 18-63 1.41e-05

Antidote-toxin recognition MazE, bacterial antitoxin; AbrB-like is a family of small proteins that operate in conjunction with a cognate toxin molecule. The commonly attributed role of toxin-antitoxin systems is to maintain low-copy number plasmids from one generation to the next. Such gene-pairs are also found on chromosomes and to be associated with a number of biological functions such as: reduction of protein synthesis, gene regulation and retardation of cell growth under nutritional stress. This family includes proteins from a number of different pairings, eg MazE, AbrB, VapB, PhoU, PemI-like and SpoVT. MazE is the antidote to the toxin MazF of E. coli. MazE-MazF in E. coli is a regulated prokaryotic chromosomal addiction module. MazE antidote is degraded by the ClpPA protease of the bacterial proteasome. MazE-MazF is thought to play a role in programmed cell death when cells suffer nutrient deprivation, and MazE-MazF modules have also been implicated in the bacteriostatic effects of other addiction modules.


Pssm-ID: 397909 [Multi-domain]  Cd Length: 44  Bit Score: 38.65  E-value: 1.41e-05
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*.
gi 446048548  18 TKIGNSLGLTLPQEFYTTLNIKQGDVVSLEIneENEQIVIKKSKKI 63
Cdd:pfam04014  1 FKVGNSGAVRIPKEVRKALGIKEGDEVELEV--DGDKIILKPKKSE 44
AbrB COG2002
Bifunctional DNA-binding transcriptional regulator of stationary/sporulation/toxin gene ...
 16-62 2.02e-03

Bifunctional DNA-binding transcriptional regulator of stationary/sporulation/toxin gene expression and antitoxin component of the YhaV-PrlF toxin-antitoxin module [Transcription, Defense mechanisms];


Pssm-ID: 441605 [Multi-domain]  Cd Length: 70  Bit Score: 33.80  E-value: 2.02e-03
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*...
gi 446048548 16 KVTKIGnSLG-LTLPQEFYTTLNIKQGDVVslEINEENEQIVIKKSKK 62
Cdd:COG2002   2 KTVKVT-SKGqITIPKEIREALGIKEGDKL--EVIVDGDGIVLKPVKP 46
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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