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Conserved domains on  [gi|396591|emb|CAA52462|]
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catechol O-methyltransferase [Nicotiana tabacum]

Protein Classification

COMT family class I SAM-dependent methyltransferase( domain architecture ID 10547674)

COMT family class I SAM-dependent methyltransferase catalyzes the methylation of one or more specific substrates using S-adenosyl-L-methionine (SAM or AdoMet) as the methyl donor; similar to caffeic acid 3-O-methyltransferase (COMT) that catalyzes the conversion of caffeic acid to ferulic acid and of 5-hydroxyferulic acid to sinapic acid

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Methyltransf_2 pfam00891
O-methyltransferase domain; This family includes a range of O-methyltransferases. These ...
 140-344 9.67e-104

O-methyltransferase domain; This family includes a range of O-methyltransferases. These enzymes utilize S-adenosyl methionine.


:

Pssm-ID: 395719 [Multi-domain]  Cd Length: 208  Bit Score: 303.94  E-value: 9.67e-104
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 396591     140 WYHLKDAVLDGGIPFNKAYGMTAFEYHGTDPRFNKVFNRGMSDHSTMSMKKILEDYKgFEGLNSIVDVGGGTGATVNMIV 219
Cdd:pfam00891   1 WRYLADAVREGRNQYNKAFGISLFEAIYRDEEERLLFNRGLQEHWSLIGKDVLTAFD-LSGFRSLVDVGGGTGALAQAIV 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 396591     220 SKHPSIKGINFDLPHVIGDAPAY------PGVEHVGGDMFA-SVPKADAIFMKWICHDWSDEHCLKFLKNCYEALPANGK 292
Cdd:pfam00891  80 SLYPGCKGIVFDLPHVVEAAPTHfsageePRVTFHGGDFFKdSLPEADAYILKRVLHDWSDEKCVKLLKRCYKACPAGGK 159

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|..
gi 396591     293 VIIAECILPEAPDTSLATKNtvhVDIVMLAHnPGGKERTEKEFEALAKGAGF 344
Cdd:pfam00891 160 VILVESLLGADPSGPLHTQL---YSLNMLAQ-TEGRERTEAEYSELLTGAGF 207
dimerization pfam08100
dimerization domain; This domain is found at the N-terminus of a variety of plant ...
 34-85 1.02e-18

dimerization domain; This domain is found at the N-terminus of a variety of plant O-methyltransferases. It has been shown to mediate dimerization of these proteins.


:

Pssm-ID: 400439  Cd Length: 50  Bit Score: 78.77  E-value: 1.02e-18
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|..
gi 396591      34 MVLKSALELDLLELMAKAGpgAAISPSELAAQLSTQNPEAPVILDRMLRLLA 85
Cdd:pfam08100   1 MVLKCAIELGIPDIIAKHG--KPLSPSELASKLPTKNPEAPVMLDRLLRLLA 50
 
Name Accession Description Interval E-value
Methyltransf_2 pfam00891
O-methyltransferase domain; This family includes a range of O-methyltransferases. These ...
 140-344 9.67e-104

O-methyltransferase domain; This family includes a range of O-methyltransferases. These enzymes utilize S-adenosyl methionine.


Pssm-ID: 395719 [Multi-domain]  Cd Length: 208  Bit Score: 303.94  E-value: 9.67e-104
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 396591     140 WYHLKDAVLDGGIPFNKAYGMTAFEYHGTDPRFNKVFNRGMSDHSTMSMKKILEDYKgFEGLNSIVDVGGGTGATVNMIV 219
Cdd:pfam00891   1 WRYLADAVREGRNQYNKAFGISLFEAIYRDEEERLLFNRGLQEHWSLIGKDVLTAFD-LSGFRSLVDVGGGTGALAQAIV 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 396591     220 SKHPSIKGINFDLPHVIGDAPAY------PGVEHVGGDMFA-SVPKADAIFMKWICHDWSDEHCLKFLKNCYEALPANGK 292
Cdd:pfam00891  80 SLYPGCKGIVFDLPHVVEAAPTHfsageePRVTFHGGDFFKdSLPEADAYILKRVLHDWSDEKCVKLLKRCYKACPAGGK 159

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|..
gi 396591     293 VIIAECILPEAPDTSLATKNtvhVDIVMLAHnPGGKERTEKEFEALAKGAGF 344
Cdd:pfam00891 160 VILVESLLGADPSGPLHTQL---YSLNMLAQ-TEGRERTEAEYSELLTGAGF 207
dimerization pfam08100
dimerization domain; This domain is found at the N-terminus of a variety of plant ...
 34-85 1.02e-18

dimerization domain; This domain is found at the N-terminus of a variety of plant O-methyltransferases. It has been shown to mediate dimerization of these proteins.


Pssm-ID: 400439  Cd Length: 50  Bit Score: 78.77  E-value: 1.02e-18
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|..
gi 396591      34 MVLKSALELDLLELMAKAGpgAAISPSELAAQLSTQNPEAPVILDRMLRLLA 85
Cdd:pfam08100   1 MVLKCAIELGIPDIIAKHG--KPLSPSELASKLPTKNPEAPVMLDRLLRLLA 50
Tam COG4106
Trans-aconitate methyltransferase [Energy production and conversion];
203-297 4.50e-05

Trans-aconitate methyltransferase [Energy production and conversion];


Pssm-ID: 443282 [Multi-domain]  Cd Length: 100  Bit Score: 41.73  E-value: 4.50e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 396591   203 SIVDVGGGTGATVNMIVSKHPSIKGINFDL-PHVIGDAPA-YPGVEHVGGDM--FASVPKADAIFMKWICHdWSDEHcLK 278
Cdd:COG4106   4 RVLDLGCGTGRLTALLAERFPGARVTGVDLsPEMLARARArLPNVRFVVADLrdLDPPEPFDLVVSNAALH-WLPDH-AA 81

                        90
                ....*....|....*....
gi 396591   279 FLKNCYEALPANGKVIIAE 297
Cdd:COG4106  82 LLARLAAALAPGGVLAVQV 100
AdoMet_MTases cd02440
S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; ...
 204-295 1.53e-04

S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). There are at least five structurally distinct families of AdoMet-MTases, class I being the largest and most diverse. Within this class enzymes can be classified by different substrate specificities (small molecules, lipids, nucleic acids, etc.) and different target atoms for methylation (nitrogen, oxygen, carbon, sulfur, etc.).


Pssm-ID: 100107 [Multi-domain]  Cd Length: 107  Bit Score: 40.49  E-value: 1.53e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 396591   204 IVDVGGGTGATVNMIVSkHPSIKGINFDL-PHVIGDAPA------YPGVEHVGGDM----FASVPKADAIFMKWICHdWS 272
Cdd:cd02440   2 VLDLGCGTGALALALAS-GPGARVTGVDIsPVALELARKaaaallADNVEVLKGDAeelpPEADESFDVIISDPPLH-HL 79

                        90       100
                ....*....|....*....|...
gi 396591   273 DEHCLKFLKNCYEALPANGKVII 295
Cdd:cd02440  80 VEDLARFLEEARRLLKPGGVLVL 102
PRK06922 PRK06922
class I SAM-dependent methyltransferase;
183-306 2.45e-03

class I SAM-dependent methyltransferase;


Pssm-ID: 180751 [Multi-domain]  Cd Length: 677  Bit Score: 39.85  E-value: 2.45e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 396591    183 HSTMSMKKILEDY-KGfeglNSIVDVGGGTGATVNMIVSKHPSIKGINFDLP------------------HVI-GDA--- 239
Cdd:PRK06922 404 NSSADDKRIILDYiKG----DTIVDVGAGGGVMLDMIEEETEDKRIYGIDISenvidtlkkkkqnegrswNVIkGDAinl 479

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 396591    240 PAYPGVEHVGGDMFASVPKADAIFMKWICHDWSDEHCLKFLKNCYEALPANGKVIIAECILPEAPDT 306
Cdd:PRK06922 480 SSSFEKESVDTIVYSSILHELFSYIEYEGKKFNHEVIKKGLQSAYEVLKPGGRIIIRDGIMTEDKRL 546
methyltran_NanM TIGR04371
putative sugar O-methyltransferase; Members of this family appear to be SAM-dependent ...
 188-242 6.98e-03

putative sugar O-methyltransferase; Members of this family appear to be SAM-dependent O-methyltransferases acting on sugars, based on iterated sequence searches and gene context. Members occur in Leptospira O-antigen regions, as well NanM from the biosynthesis cluster for nanchangmycin, which produces 4-O-methyl-L-rhodinose as an intermediate. [Cell envelope, Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides]


Pssm-ID: 275164  Cd Length: 273  Bit Score: 37.78  E-value: 6.98e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 396591     188 MKKILEDYKGFeglnSIVDVGGGTGATVNMIVSKHPSIKGINFDLPHVIGDAPAY 242
Cdd:TIGR04371 141 LKKFFGDLSVF----RVLEIGGGYGRLGEILLKLFPNAIYYIVDLPPQLALSEAY 191
 
Name Accession Description Interval E-value
Methyltransf_2 pfam00891
O-methyltransferase domain; This family includes a range of O-methyltransferases. These ...
 140-344 9.67e-104

O-methyltransferase domain; This family includes a range of O-methyltransferases. These enzymes utilize S-adenosyl methionine.


Pssm-ID: 395719 [Multi-domain]  Cd Length: 208  Bit Score: 303.94  E-value: 9.67e-104
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 396591     140 WYHLKDAVLDGGIPFNKAYGMTAFEYHGTDPRFNKVFNRGMSDHSTMSMKKILEDYKgFEGLNSIVDVGGGTGATVNMIV 219
Cdd:pfam00891   1 WRYLADAVREGRNQYNKAFGISLFEAIYRDEEERLLFNRGLQEHWSLIGKDVLTAFD-LSGFRSLVDVGGGTGALAQAIV 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 396591     220 SKHPSIKGINFDLPHVIGDAPAY------PGVEHVGGDMFA-SVPKADAIFMKWICHDWSDEHCLKFLKNCYEALPANGK 292
Cdd:pfam00891  80 SLYPGCKGIVFDLPHVVEAAPTHfsageePRVTFHGGDFFKdSLPEADAYILKRVLHDWSDEKCVKLLKRCYKACPAGGK 159

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|..
gi 396591     293 VIIAECILPEAPDTSLATKNtvhVDIVMLAHnPGGKERTEKEFEALAKGAGF 344
Cdd:pfam00891 160 VILVESLLGADPSGPLHTQL---YSLNMLAQ-TEGRERTEAEYSELLTGAGF 207
dimerization pfam08100
dimerization domain; This domain is found at the N-terminus of a variety of plant ...
 34-85 1.02e-18

dimerization domain; This domain is found at the N-terminus of a variety of plant O-methyltransferases. It has been shown to mediate dimerization of these proteins.


Pssm-ID: 400439  Cd Length: 50  Bit Score: 78.77  E-value: 1.02e-18
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|..
gi 396591      34 MVLKSALELDLLELMAKAGpgAAISPSELAAQLSTQNPEAPVILDRMLRLLA 85
Cdd:pfam08100   1 MVLKCAIELGIPDIIAKHG--KPLSPSELASKLPTKNPEAPVMLDRLLRLLA 50
Tam COG4106
Trans-aconitate methyltransferase [Energy production and conversion];
203-297 4.50e-05

Trans-aconitate methyltransferase [Energy production and conversion];


Pssm-ID: 443282 [Multi-domain]  Cd Length: 100  Bit Score: 41.73  E-value: 4.50e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 396591   203 SIVDVGGGTGATVNMIVSKHPSIKGINFDL-PHVIGDAPA-YPGVEHVGGDM--FASVPKADAIFMKWICHdWSDEHcLK 278
Cdd:COG4106   4 RVLDLGCGTGRLTALLAERFPGARVTGVDLsPEMLARARArLPNVRFVVADLrdLDPPEPFDLVVSNAALH-WLPDH-AA 81

                        90
                ....*....|....*....
gi 396591   279 FLKNCYEALPANGKVIIAE 297
Cdd:COG4106  82 LLARLAAALAPGGVLAVQV 100
AdoMet_MTases cd02440
S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; ...
 204-295 1.53e-04

S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). There are at least five structurally distinct families of AdoMet-MTases, class I being the largest and most diverse. Within this class enzymes can be classified by different substrate specificities (small molecules, lipids, nucleic acids, etc.) and different target atoms for methylation (nitrogen, oxygen, carbon, sulfur, etc.).


Pssm-ID: 100107 [Multi-domain]  Cd Length: 107  Bit Score: 40.49  E-value: 1.53e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 396591   204 IVDVGGGTGATVNMIVSkHPSIKGINFDL-PHVIGDAPA------YPGVEHVGGDM----FASVPKADAIFMKWICHdWS 272
Cdd:cd02440   2 VLDLGCGTGALALALAS-GPGARVTGVDIsPVALELARKaaaallADNVEVLKGDAeelpPEADESFDVIISDPPLH-HL 79

                        90       100
                ....*....|....*....|...
gi 396591   273 DEHCLKFLKNCYEALPANGKVII 295
Cdd:cd02440  80 VEDLARFLEEARRLLKPGGVLVL 102
PRK06922 PRK06922
class I SAM-dependent methyltransferase;
183-306 2.45e-03

class I SAM-dependent methyltransferase;


Pssm-ID: 180751 [Multi-domain]  Cd Length: 677  Bit Score: 39.85  E-value: 2.45e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 396591    183 HSTMSMKKILEDY-KGfeglNSIVDVGGGTGATVNMIVSKHPSIKGINFDLP------------------HVI-GDA--- 239
Cdd:PRK06922 404 NSSADDKRIILDYiKG----DTIVDVGAGGGVMLDMIEEETEDKRIYGIDISenvidtlkkkkqnegrswNVIkGDAinl 479

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 396591    240 PAYPGVEHVGGDMFASVPKADAIFMKWICHDWSDEHCLKFLKNCYEALPANGKVIIAECILPEAPDT 306
Cdd:PRK06922 480 SSSFEKESVDTIVYSSILHELFSYIEYEGKKFNHEVIKKGLQSAYEVLKPGGRIIIRDGIMTEDKRL 546
methyltran_NanM TIGR04371
putative sugar O-methyltransferase; Members of this family appear to be SAM-dependent ...
 188-242 6.98e-03

putative sugar O-methyltransferase; Members of this family appear to be SAM-dependent O-methyltransferases acting on sugars, based on iterated sequence searches and gene context. Members occur in Leptospira O-antigen regions, as well NanM from the biosynthesis cluster for nanchangmycin, which produces 4-O-methyl-L-rhodinose as an intermediate. [Cell envelope, Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides]


Pssm-ID: 275164  Cd Length: 273  Bit Score: 37.78  E-value: 6.98e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 396591     188 MKKILEDYKGFeglnSIVDVGGGTGATVNMIVSKHPSIKGINFDLPHVIGDAPAY 242
Cdd:TIGR04371 141 LKKFFGDLSVF----RVLEIGGGYGRLGEILLKLFPNAIYYIVDLPPQLALSEAY 191
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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