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Conserved domains on  [gi|381354248|gb|AFG25846|]
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nuclear RNA polymerase I second-largest subunit, partial [Sidalcea hartwegii]

Protein Classification

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
RNA_pol_Rpb2_6 super family cl47566
RNA polymerase Rpb2, domain 6; RNA polymerases catalyze the DNA dependent polymerization of ...
1-54 1.04e-06

RNA polymerase Rpb2, domain 6; RNA polymerases catalyze the DNA dependent polymerization of RNA. Prokaryotes contain a single RNA polymerase compared to three in eukaryotes (not including mitochondrial. and chloroplast polymerases). This domain represents the hybrid binding domain and the wall domain. The hybrid binding domain binds the nascent RNA strand / template DNA strand in the Pol II transcription elongation complex. This domain contains the important structural motifs, switch 3 and the flap loop and binds an active site metal ion. This domain is also involved in binding to Rpb1 and Rpb3. Many of the bacterial members contain large insertions within this domain, as region known as dispensable region 2 (DRII).


The actual alignment was detected with superfamily member pfam00562:

Pssm-ID: 459854 [Multi-domain]  Cd Length: 371  Bit Score: 42.90  E-value: 1.04e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 381354248    1 MLLESVAAKGGSLHGKSVDATPFgdvakgtdgkteTESESLVDELGSMLRARGF 54
Cdd:pfam00562 270 QLLETHLGKAAALLGVFVDATPF------------DGASTEVEDIGELLEKAGY 311
 
Name Accession Description Interval E-value
RNA_pol_Rpb2_6 pfam00562
RNA polymerase Rpb2, domain 6; RNA polymerases catalyze the DNA dependent polymerization of ...
1-54 1.04e-06

RNA polymerase Rpb2, domain 6; RNA polymerases catalyze the DNA dependent polymerization of RNA. Prokaryotes contain a single RNA polymerase compared to three in eukaryotes (not including mitochondrial. and chloroplast polymerases). This domain represents the hybrid binding domain and the wall domain. The hybrid binding domain binds the nascent RNA strand / template DNA strand in the Pol II transcription elongation complex. This domain contains the important structural motifs, switch 3 and the flap loop and binds an active site metal ion. This domain is also involved in binding to Rpb1 and Rpb3. Many of the bacterial members contain large insertions within this domain, as region known as dispensable region 2 (DRII).


Pssm-ID: 459854 [Multi-domain]  Cd Length: 371  Bit Score: 42.90  E-value: 1.04e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 381354248    1 MLLESVAAKGGSLHGKSVDATPFgdvakgtdgkteTESESLVDELGSMLRARGF 54
Cdd:pfam00562 270 QLLETHLGKAAALLGVFVDATPF------------DGASTEVEDIGELLEKAGY 311
PRK08565 PRK08565
DNA-directed RNA polymerase subunit B; Provisional
1-54 2.16e-05

DNA-directed RNA polymerase subunit B; Provisional


Pssm-ID: 236291 [Multi-domain]  Cd Length: 1103  Bit Score: 39.17  E-value: 2.16e-05
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 381354248    1 MLLESVAAKGGSLHGKSVDATPFgdvakgtdgktetESESlVDELGSMLRARGF 54
Cdd:PRK08565  907 QLLESIAGKVAALEGRFVDATPF-------------YGEP-EEELRKELLKLGY 946
 
Name Accession Description Interval E-value
RNA_pol_Rpb2_6 pfam00562
RNA polymerase Rpb2, domain 6; RNA polymerases catalyze the DNA dependent polymerization of ...
1-54 1.04e-06

RNA polymerase Rpb2, domain 6; RNA polymerases catalyze the DNA dependent polymerization of RNA. Prokaryotes contain a single RNA polymerase compared to three in eukaryotes (not including mitochondrial. and chloroplast polymerases). This domain represents the hybrid binding domain and the wall domain. The hybrid binding domain binds the nascent RNA strand / template DNA strand in the Pol II transcription elongation complex. This domain contains the important structural motifs, switch 3 and the flap loop and binds an active site metal ion. This domain is also involved in binding to Rpb1 and Rpb3. Many of the bacterial members contain large insertions within this domain, as region known as dispensable region 2 (DRII).


Pssm-ID: 459854 [Multi-domain]  Cd Length: 371  Bit Score: 42.90  E-value: 1.04e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 381354248    1 MLLESVAAKGGSLHGKSVDATPFgdvakgtdgkteTESESLVDELGSMLRARGF 54
Cdd:pfam00562 270 QLLETHLGKAAALLGVFVDATPF------------DGASTEVEDIGELLEKAGY 311
PRK08565 PRK08565
DNA-directed RNA polymerase subunit B; Provisional
1-54 2.16e-05

DNA-directed RNA polymerase subunit B; Provisional


Pssm-ID: 236291 [Multi-domain]  Cd Length: 1103  Bit Score: 39.17  E-value: 2.16e-05
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 381354248    1 MLLESVAAKGGSLHGKSVDATPFgdvakgtdgktetESESlVDELGSMLRARGF 54
Cdd:PRK08565  907 QLLESIAGKVAALEGRFVDATPF-------------YGEP-EEELRKELLKLGY 946
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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