NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|336359746|gb|AEI53609|]
View 

aminoglycoside phosphotransferase APH (3')-IIIA [Expression vector lacI-ptac-gusA-pBAV1k]

Protein Classification

aminoglycoside 3'-phosphotransferase( domain architecture ID 10790026)

aminoglycoside 3'-phosphotransferase phosphorylates and inactives antibiotic substrates such as kanamycin, streptomycin, neomycin, and gentamicin, among others

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
Aph COG3231
Aminoglycoside phosphotransferase [Translation, ribosomal structure and biogenesis];
6-220 7.37e-93

Aminoglycoside phosphotransferase [Translation, ribosomal structure and biogenesis];


:

Pssm-ID: 442463 [Multi-domain]  Cd Length: 258  Bit Score: 272.56  E-value: 7.37e-93
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746   6 YKGTTYDVEREKDMMLWLEGK-LPVPKVLHFERHDGWSNLLMSEADGVLCSEGYEdEQSPEKTIELYAECIRLFHSIDIS 84
Cdd:COG3231   46 PAGPAAELEDEADRLRWLAGQgLPVPEVLDFGEDDGGAWLLTTAVPGRPAASVSE-ALDPERAVELLAEALRRLHALPVA 124

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746  85 DCPYTNSLDGRLAELDYLLNNDLadVDCENWEEDTPFKDPRELYDFLKTEKP-EEELVFSHGDLGDSNIFVKDGKVSGFI 163
Cdd:COG3231  125 DCPFDRRLERRLAEARARVAAGL--VDPDDFDEERRGRPPEELLAELLAERPaEEDLVVTHGDACLPNILVDPGTFSGFI 202

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 336359746 164 DLGRSGRADKWYDIAFCVRSIREDIGEEqYVELFFDLLGIKPDWEKIKYYILLDELF 220
Cdd:COG3231  203 DLGRLGVADRYQDLALAARSLRENLGEG-WVEPFLDAYGIAPDPERLAFYRLLDEFF 258
 
Name Accession Description Interval E-value
Aph COG3231
Aminoglycoside phosphotransferase [Translation, ribosomal structure and biogenesis];
6-220 7.37e-93

Aminoglycoside phosphotransferase [Translation, ribosomal structure and biogenesis];


Pssm-ID: 442463 [Multi-domain]  Cd Length: 258  Bit Score: 272.56  E-value: 7.37e-93
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746   6 YKGTTYDVEREKDMMLWLEGK-LPVPKVLHFERHDGWSNLLMSEADGVLCSEGYEdEQSPEKTIELYAECIRLFHSIDIS 84
Cdd:COG3231   46 PAGPAAELEDEADRLRWLAGQgLPVPEVLDFGEDDGGAWLLTTAVPGRPAASVSE-ALDPERAVELLAEALRRLHALPVA 124

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746  85 DCPYTNSLDGRLAELDYLLNNDLadVDCENWEEDTPFKDPRELYDFLKTEKP-EEELVFSHGDLGDSNIFVKDGKVSGFI 163
Cdd:COG3231  125 DCPFDRRLERRLAEARARVAAGL--VDPDDFDEERRGRPPEELLAELLAERPaEEDLVVTHGDACLPNILVDPGTFSGFI 202

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 336359746 164 DLGRSGRADKWYDIAFCVRSIREDIGEEqYVELFFDLLGIKPDWEKIKYYILLDELF 220
Cdd:COG3231  203 DLGRLGVADRYQDLALAARSLRENLGEG-WVEPFLDAYGIAPDPERLAFYRLLDEFF 258
APH cd05150
Aminoglycoside 3'-phosphotransferase; APH catalyzes the transfer of the gamma-phosphoryl group ...
 7-220 3.07e-87

Aminoglycoside 3'-phosphotransferase; APH catalyzes the transfer of the gamma-phosphoryl group from ATP to aminoglycoside antibiotics such as kanamycin, streptomycin, neomycin, and gentamicin, among others. The aminoglycoside antibiotics target the 30S ribosome and promote miscoding, leading to the production of defective proteins which insert into the bacterial membrane, resulting in membrane damage and the ultimate demise of the bacterium. Phosphorylation of the aminoglycoside antibiotics results in their inactivation, leading to bacterial antibiotic resistance. The APH gene is found on transposons and plasmids and is thought to have originated as a self-defense mechanism used by microorganisms that produce the antibiotics. The APH subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270699 [Multi-domain]  Cd Length: 244  Bit Score: 257.51  E-value: 3.07e-87
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746   7 KGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEGYEDEQsPEKTIELYAECIRLFHSIDISDC 86
Cdd:cd05150   32 AGYAYELAREAERLRWLAGKLPVPEVLDYGSDDGGDWLLTTALPGRDAASLEPLLD-PERLVDLLAEALRALHSLPIADC 110

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746  87 PYTNSLDGRLAELDYLLNNDLADVDceNWEEDTPFKDPRELYDFLKTEKPEEE-LVFSHGDLGDSNIFVKDGKVSGFIDL 165
Cdd:cd05150  111 PFDRRLDARLAEARARVEAGLVDED--DFDEERQGRTAEELLAELEATRPAEEdLVVTHGDACLPNIILDPGRFSGFIDL 188

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 336359746 166 GRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGI-KPDWEKIKYYILLDELF 220
Cdd:cd05150  189 GRLGVADRYQDLALAVRSLRENLGGEEYAERFLDAYGIdAPDPERLAYYRLLDEFF 244
APH pfam01636
Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance ...
 12-213 1.97e-22

Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance proteins, which confer resistance to various aminoglycosides they include: aminoglycoside 3'-phosphotransferase or kanamycin kinase / neomycin-kanamycin phosphotransferase and streptomycin 3''-kinase or streptomycin 3''-phosphotransferase. The aminoglycoside phosphotransferases inactivate aminoglycoside antibiotics via phosphorylation. This family also includes homoserine kinase. This family is related to fructosamine kinase pfam03881.


Pssm-ID: 426359 [Multi-domain]  Cd Length: 239  Bit Score: 91.41  E-value: 1.97e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746   12 DVEREKDMMLWL--EGKLPVPKVLHFER---HDGWSNLLMSEADGVLCSEGYEDEQSPEkTIELYAECIRLFHSIDISDC 86
Cdd:pfam01636  36 ELRRELALLRHLaaAGVPPVPRVLAGCTdaeLLGLPFLLMEYLPGEVLARPLLPEERGA-LLEALGRALARLHAVDPAAL 114

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746   87 PYTNSLDGRLAELDYLLNNDLADVDCENweEDTPFKDPRELYDFLKTEKP-EEELVFSHGDLGDSNIFV-KDGKVSGFID 164
Cdd:pfam01636 115 PLAGRLARLLELLRQLEAALARLLAAEL--LDRLEELEERLLAALLALLPaELPPVLVHGDLHPGNLLVdPGGRVSGVID 192

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*....
gi 336359746  165 LGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLgiKPDWEKIKYY 213
Cdd:pfam01636 193 FEDAGLGDPAYDLAILLNSWGRELGAELLAAYLAAYG--AFGYARLREL 239
 
Name Accession Description Interval E-value
Aph COG3231
Aminoglycoside phosphotransferase [Translation, ribosomal structure and biogenesis];
6-220 7.37e-93

Aminoglycoside phosphotransferase [Translation, ribosomal structure and biogenesis];


Pssm-ID: 442463 [Multi-domain]  Cd Length: 258  Bit Score: 272.56  E-value: 7.37e-93
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746   6 YKGTTYDVEREKDMMLWLEGK-LPVPKVLHFERHDGWSNLLMSEADGVLCSEGYEdEQSPEKTIELYAECIRLFHSIDIS 84
Cdd:COG3231   46 PAGPAAELEDEADRLRWLAGQgLPVPEVLDFGEDDGGAWLLTTAVPGRPAASVSE-ALDPERAVELLAEALRRLHALPVA 124

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746  85 DCPYTNSLDGRLAELDYLLNNDLadVDCENWEEDTPFKDPRELYDFLKTEKP-EEELVFSHGDLGDSNIFVKDGKVSGFI 163
Cdd:COG3231  125 DCPFDRRLERRLAEARARVAAGL--VDPDDFDEERRGRPPEELLAELLAERPaEEDLVVTHGDACLPNILVDPGTFSGFI 202

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 336359746 164 DLGRSGRADKWYDIAFCVRSIREDIGEEqYVELFFDLLGIKPDWEKIKYYILLDELF 220
Cdd:COG3231  203 DLGRLGVADRYQDLALAARSLRENLGEG-WVEPFLDAYGIAPDPERLAFYRLLDEFF 258
APH cd05150
Aminoglycoside 3'-phosphotransferase; APH catalyzes the transfer of the gamma-phosphoryl group ...
 7-220 3.07e-87

Aminoglycoside 3'-phosphotransferase; APH catalyzes the transfer of the gamma-phosphoryl group from ATP to aminoglycoside antibiotics such as kanamycin, streptomycin, neomycin, and gentamicin, among others. The aminoglycoside antibiotics target the 30S ribosome and promote miscoding, leading to the production of defective proteins which insert into the bacterial membrane, resulting in membrane damage and the ultimate demise of the bacterium. Phosphorylation of the aminoglycoside antibiotics results in their inactivation, leading to bacterial antibiotic resistance. The APH gene is found on transposons and plasmids and is thought to have originated as a self-defense mechanism used by microorganisms that produce the antibiotics. The APH subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270699 [Multi-domain]  Cd Length: 244  Bit Score: 257.51  E-value: 3.07e-87
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746   7 KGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEGYEDEQsPEKTIELYAECIRLFHSIDISDC 86
Cdd:cd05150   32 AGYAYELAREAERLRWLAGKLPVPEVLDYGSDDGGDWLLTTALPGRDAASLEPLLD-PERLVDLLAEALRALHSLPIADC 110

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746  87 PYTNSLDGRLAELDYLLNNDLADVDceNWEEDTPFKDPRELYDFLKTEKPEEE-LVFSHGDLGDSNIFVKDGKVSGFIDL 165
Cdd:cd05150  111 PFDRRLDARLAEARARVEAGLVDED--DFDEERQGRTAEELLAELEATRPAEEdLVVTHGDACLPNIILDPGRFSGFIDL 188

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 336359746 166 GRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGI-KPDWEKIKYYILLDELF 220
Cdd:cd05150  189 GRLGVADRYQDLALAVRSLRENLGGEEYAERFLDAYGIdAPDPERLAYYRLLDEFF 244
PKc_like cd13968
Catalytic domain of the Protein Kinase superfamily; The PK superfamily contains the large ...
 1-166 1.95e-24

Catalytic domain of the Protein Kinase superfamily; The PK superfamily contains the large family of typical PKs that includes serine/threonine kinases (STKs), protein tyrosine kinases (PTKs), and dual-specificity PKs that phosphorylate both serine/threonine and tyrosine residues of target proteins, as well as pseudokinases that lack crucial residues for catalytic activity and/or ATP binding. It also includes phosphoinositide 3-kinases (PI3Ks), aminoglycoside 3'-phosphotransferases (APHs), choline kinase (ChoK), Actin-Fragmin Kinase (AFK), and the atypical RIO and Abc1p-like protein kinases. These proteins catalyze the transfer of the gamma-phosphoryl group from ATP to their target substrates; these include serine/threonine/tyrosine residues in proteins for typical or atypical PKs, the 3-hydroxyl of the inositol ring of D-myo-phosphatidylinositol (PtdIns) or its derivatives for PI3Ks, the 4-hydroxyl of PtdIns for PI4Ks, and other small molecule substrates for APH/ChoK and similar proteins such as aminoglycosides, macrolides, choline, ethanolamine, and homoserine.


Pssm-ID: 270870 [Multi-domain]  Cd Length: 136  Bit Score: 93.66  E-value: 1.95e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746   1 MTDSRYKGTTYDVEREKDMMLWLEGK-LPVPKVLHFERHDGWSNLLMSEADGVLCSE----GYEDEQSPEKTIELYAECI 75
Cdd:cd13968   25 IGDDVNNEEGEDLESEMDILRRLKGLeLNIPKVLVTEDVDGPNILLMELVKGGTLIAytqeEELDEKDVESIMYQLAECM 104

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746  76 RLFHSIdisdcpytnsldgrlaeldyllnndladvdcenweedtpfkdprelydflktekpeeelVFSHGDLGDSNIFVK 155
Cdd:cd13968  105 RLLHSF-----------------------------------------------------------HLIHRDLNNDNILLS 125

                        170
                 ....*....|.
gi 336359746 156 DGKVSGFIDLG 166
Cdd:cd13968  126 EDGNVKLIDFG 136
APH_ChoK_like cd05120
Aminoglycoside 3'-phosphotransferase and Choline Kinase family; This family is composed of APH, ...
 11-186 6.25e-24

Aminoglycoside 3'-phosphotransferase and Choline Kinase family; This family is composed of APH, ChoK, ethanolamine kinase (ETNK), macrolide 2'-phosphotransferase (MPH2'), an unusual homoserine kinase, and uncharacterized proteins with similarity to the N-terminal domain of acyl-CoA dehydrogenase 10 (ACAD10). The members of this family catalyze the transfer of the gamma-phosphoryl group from ATP (or CTP) to small molecule substrates such as aminoglycosides, macrolides, choline, ethanolamine, and homoserine. Phosphorylation of the antibiotics, aminoglycosides and macrolides, leads to their inactivation and to bacterial antibiotic resistance. Phosphorylation of choline, ethanolamine, and homoserine serves as precursors to the synthesis of important biological compounds, such as the major phospholipids, phosphatidylcholine and phosphatidylethanolamine and the amino acids, threonine, methionine, and isoleucine. The APH/ChoK family is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270690 [Multi-domain]  Cd Length: 158  Bit Score: 93.14  E-value: 6.25e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746  11 YDVEREKDMMLWLEGK--LPVPKVLHFERHDGWSNLLMSEADGVLCSEGY--EDEQSPEKTIELYAECIRLFHSIDISdc 86
Cdd:cd05120   34 KDLEKEAAMLQLLAGKlsLPVPKVYGFGESDGWEYLLMERIEGETLSEVWprLSEEEKEKIADQLAEILAALHRIDSS-- 111

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746  87 pytnsldgrlaeldyllnndladvdcenweedtpfkdprelydflktekpeeelVFSHGDLGDSNIFVKD-GKVSGFIDL 165
Cdd:cd05120  112 ------------------------------------------------------VLTHGDLHPGNILVKPdGKLSGIIDW 137

                        170       180
                 ....*....|....*....|.
gi 336359746 166 GRSGRADKWYDIAFCVRSIRE 186
Cdd:cd05120  138 EFAGYGPPAFDYAAALRDWTE 158
APH pfam01636
Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance ...
 12-213 1.97e-22

Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance proteins, which confer resistance to various aminoglycosides they include: aminoglycoside 3'-phosphotransferase or kanamycin kinase / neomycin-kanamycin phosphotransferase and streptomycin 3''-kinase or streptomycin 3''-phosphotransferase. The aminoglycoside phosphotransferases inactivate aminoglycoside antibiotics via phosphorylation. This family also includes homoserine kinase. This family is related to fructosamine kinase pfam03881.


Pssm-ID: 426359 [Multi-domain]  Cd Length: 239  Bit Score: 91.41  E-value: 1.97e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746   12 DVEREKDMMLWL--EGKLPVPKVLHFER---HDGWSNLLMSEADGVLCSEGYEDEQSPEkTIELYAECIRLFHSIDISDC 86
Cdd:pfam01636  36 ELRRELALLRHLaaAGVPPVPRVLAGCTdaeLLGLPFLLMEYLPGEVLARPLLPEERGA-LLEALGRALARLHAVDPAAL 114

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746   87 PYTNSLDGRLAELDYLLNNDLADVDCENweEDTPFKDPRELYDFLKTEKP-EEELVFSHGDLGDSNIFV-KDGKVSGFID 164
Cdd:pfam01636 115 PLAGRLARLLELLRQLEAALARLLAAEL--LDRLEELEERLLAALLALLPaELPPVLVHGDLHPGNLLVdPGGRVSGVID 192

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*....
gi 336359746  165 LGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLgiKPDWEKIKYY 213
Cdd:pfam01636 193 FEDAGLGDPAYDLAILLNSWGRELGAELLAAYLAAYG--AFGYARLREL 239
YcbJ COG3173
Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction ...
 5-220 4.11e-13

Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction only];


Pssm-ID: 442406 [Multi-domain]  Cd Length: 284  Bit Score: 66.68  E-value: 4.11e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746   5 RYKGTTYDVEREKDMMLWLEGKL--PVPKVLHFERHD---GWSNLLMSEADGVLCSEGYEDEqSPEKTIELYAECIRLF- 78
Cdd:COG3173   52 RGLASAHDVRREARVLRALAPRLgvPVPRPLALGEDGeviGAPFYVMEWVEGETLEDALPDL-SPAERRALARALGEFLa 130

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746  79 --HSIDISDCPYTnslDGRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEE-ELVFSHGDLGDSNIFV- 154
Cdd:COG3173  131 alHAVDPAAAGLA---DGRPEGLERQLARWRAQLRRALARTDDLPALRERLAAWLAANLPEWgPPVLVHGDLRPGNLLVd 207

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 336359746 155 -KDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIG----EEQYVELFFDLLGIKPDWEkikYYILLDELF 220
Cdd:COG3173  208 pDDGRLTAVIDWELATLGDPAADLAYLLLYWRLPDDllgpRAAFLAAYEEATGDLDDLT---WWALADPEL 275
SrkA COG2334
Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist [Signal ...
 144-199 1.71e-05

Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist [Signal transduction mechanisms]; Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist is part of the Pathway/BioSystem: Threonine biosynthesis


Pssm-ID: 441905 [Multi-domain]  Cd Length: 297  Bit Score: 44.53  E-value: 1.71e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 336359746 144 HGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFD 199
Cdd:COG2334  183 HGDLHPDNVLFDGDGVSGLIDFDDAGYGPRLYDLAIALNGWADGPLDPARLAALLE 238
HomoserineK_II cd05153
Type II Homoserine Kinase; This subfamily is composed of unusual homoserine kinases, from a ...
 144-199 1.28e-04

Type II Homoserine Kinase; This subfamily is composed of unusual homoserine kinases, from a subset of bacteria, which have a Protein Kinase fold. These proteins do not bear any similarity to the GHMP family homoserine kinases present in most bacteria and eukaryotes. Homoserine kinase catalyzes the transfer of the gamma-phosphoryl group from ATP to L-homoserine producing L-homoserine phosphate, an intermediate in the production of the amino acids threonine, methionine, and isoleucine. The Type II homoserine kinase subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270702 [Multi-domain]  Cd Length: 300  Bit Score: 41.86  E-value: 1.28e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 336359746 144 HGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA-FCVRSIREDIG--EEQYVELFFD 199
Cdd:cd05153  183 HADLFRDNVLFDGDRLSGIIDFYDACYDPLLYDLAiALNDWCFDDDGklDPERAKALLA 241
ACAD10_11_N-like cd05154
N-terminal domain of Acyl-CoA dehydrogenase (ACAD) 10 and 11, and similar proteins; This ...
 9-187 1.53e-04

N-terminal domain of Acyl-CoA dehydrogenase (ACAD) 10 and 11, and similar proteins; This subfamily is composed of the N-terminal domains of vertebrate ACAD10 and ACAD11, and similar uncharacterized bacterial and eukaryotic proteins. ACADs are a family of flavoproteins that are involved in the beta-oxidation of fatty acyl-CoA derivatives. ACAD deficiency can cause metabolic disorders including muscle fatigue, hypoglycemia, and hepatic lipidosis. There are at least 11 distinct ACADs, some of which show distinct substrate specificities to either straight-chain or branched-chain fatty acids. ACAD10 is widely expressed in human tissues and highly expressed in liver, kidney, pancreas, and spleen. ACAD10 and ACAD11 are both significantly expressed in human brain tissues. They contain a long N-terminal domain with similarity to phosphotransferases with a Protein Kinase fold, which is absent in other ACADs. They may exhibit multiple functions in acyl-CoA oxidation pathways. ACAD11 utilizes substrates with carbon chain lengths of 20 to 26, with optimal activity towards C22CoA. ACAD10 may be associated with an increased risk in type II diabetes. The ACAD10/11-like subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270703 [Multi-domain]  Cd Length: 254  Bit Score: 41.45  E-value: 1.53e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746   9 TTYDVEREKDMMLWLEGK-LPVPKVLHFE---RHDGWSNLLMSEADG-VLCSEGYEDEQSPEKTIELYAECIRLF---HS 80
Cdd:cd05154   41 SAHDLEREYRVLRALAGTgVPVPRVLALCedpSVLGAPFYVMERVDGrVLPDPLPRPDLSPEERRALARSLVDALaalHS 120

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746  81 IDISDCPytnsLDGRLAELDYLLN--NDLADVdcenWEEDTPFKDP--RELYDFLKTEKPEE-ELVFSHGDLGDSN-IFV 154
Cdd:cd05154  121 VDPAALG----LADLGRPEGYLERqvDRWRRQ----LEAAATDPPPalEEALRWLRANLPADgRPVLVHGDFRLGNlLFD 192

                        170       180       190
                 ....*....|....*....|....*....|....
gi 336359746 155 KDGKVSGFIDLGRSGRADKWYDIA-FCVRSIRED 187
Cdd:cd05154  193 PDGRVTAVLDWELATLGDPLEDLAwLLARWWRPG 226
CotS COG0510
Thiamine kinase or a related kinase [Coenzyme transport and metabolism];
125-219 3.37e-04

Thiamine kinase or a related kinase [Coenzyme transport and metabolism];


Pssm-ID: 440276 [Multi-domain]  Cd Length: 156  Bit Score: 39.76  E-value: 3.37e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746 125 RELYDFLKT--EKPEEELVFSHGDLGDSNIFV-KDGKVSgFIDLGRSGRADKWYDIA-FCVRSIREDIGEEQYVELFFDL 200
Cdd:COG0510   32 LRRLEELERalAARPLPLVLCHGDLHPGNFLVtDDGRLY-LIDWEYAGLGDPAFDLAaLLVEYGLSPEQAEELLEAYGFG 110

                         90
                 ....*....|....*....
gi 336359746 201 LGIKPDWEKIKYYILLDEL 219
Cdd:COG0510  111 RPTEELLRRLRAYRALADL 129
APH_ChoK_like_1 cd05155
Uncharacterized bacterial proteins with similarity to Aminoglycoside 3'-phosphotransferase and ...
 72-172 6.66e-04

Uncharacterized bacterial proteins with similarity to Aminoglycoside 3'-phosphotransferase and Choline kinase; This subfamily is composed of uncharacterized bacterial proteins with similarity to APH and ChoK. Other APH/ChoK-like proteins include ethanolamine kinase (ETNK), macrolide 2'-phosphotransferase (MPH2'), an unusual homoserine kinase, and uncharacterized proteins with similarity to the N-terminal domain of acyl-CoA dehydrogenase 10 (ACAD10). These proteins catalyze the transfer of the gamma-phosphoryl group from ATP (or CTP) to small molecule substrates, such as aminoglycosides, macrolides, choline, ethanolamine, and homoserine. Phosphorylation of the antibiotics, aminoglycosides, and macrolides leads to their inactivation and to bacterial antibiotic resistance. Phosphorylation of choline, ethanolamine, and homoserine serves as precursors to the synthesis of important biological compounds, such as the major phospholipids, phosphatidylcholine and phosphatidylethanolamine and the amino acids, threonine, methionine, and isoleucine. The APH/ChoK-like subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270704 [Multi-domain]  Cd Length: 234  Bit Score: 39.53  E-value: 6.66e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 336359746  72 AECIRLFHSIDISDCPytnsLDGRLAELDYLlnnDLADVDCENWEED-TPFKDPREL----YDFLKTEKPEEELVFSHGD 146
Cdd:cd05155   97 ARFLAALHAIDPAGPP----NPGRGNPLRGR---DLAVRDAEEALAAlAGLLDVAAAralwERALAAPAWAGPPVWLHGD 169

                         90       100
                 ....*....|....*....|....*.
gi 336359746 147 LGDSNIFVKDGKVSGFIDLGRSGRAD 172
Cdd:cd05155  170 LHPGNLLVRDGRLSAVIDFGDLGVGD 195
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH