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Chain A, APH(2'')-Id
phosphotransferase family protein( domain architecture ID 10007008)
phosphotransferase family protein may catalyze the phosphorylation of aminoglycosides and confer aminoglycoside antibiotic resistance; similar to viomycin phosphotransferase, 4-hydroxytryptamine kinase, and hygromycin B phosphotransferase
List of domain hits
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Name | Accession | Description | Interval | E-value | ||||
YcbJ | COG3173 | Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction ... |
7-234 | 2.99e-22 | ||||
Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction only]; : Pssm-ID: 442406 [Multi-domain] Cd Length: 284 Bit Score: 93.64 E-value: 2.99e-22
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Name | Accession | Description | Interval | E-value | ||||
YcbJ | COG3173 | Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction ... |
7-234 | 2.99e-22 | ||||
Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction only]; Pssm-ID: 442406 [Multi-domain] Cd Length: 284 Bit Score: 93.64 E-value: 2.99e-22
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APH | pfam01636 | Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance ... |
40-252 | 5.78e-22 | ||||
Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance proteins, which confer resistance to various aminoglycosides they include: aminoglycoside 3'-phosphotransferase or kanamycin kinase / neomycin-kanamycin phosphotransferase and streptomycin 3''-kinase or streptomycin 3''-phosphotransferase. The aminoglycoside phosphotransferases inactivate aminoglycoside antibiotics via phosphorylation. This family also includes homoserine kinase. This family is related to fructosamine kinase pfam03881. Pssm-ID: 426359 [Multi-domain] Cd Length: 239 Bit Score: 92.18 E-value: 5.78e-22
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APH_ChoK_like_1 | cd05155 | Uncharacterized bacterial proteins with similarity to Aminoglycoside 3'-phosphotransferase and ... |
42-226 | 1.76e-14 | ||||
Uncharacterized bacterial proteins with similarity to Aminoglycoside 3'-phosphotransferase and Choline kinase; This subfamily is composed of uncharacterized bacterial proteins with similarity to APH and ChoK. Other APH/ChoK-like proteins include ethanolamine kinase (ETNK), macrolide 2'-phosphotransferase (MPH2'), an unusual homoserine kinase, and uncharacterized proteins with similarity to the N-terminal domain of acyl-CoA dehydrogenase 10 (ACAD10). These proteins catalyze the transfer of the gamma-phosphoryl group from ATP (or CTP) to small molecule substrates, such as aminoglycosides, macrolides, choline, ethanolamine, and homoserine. Phosphorylation of the antibiotics, aminoglycosides, and macrolides leads to their inactivation and to bacterial antibiotic resistance. Phosphorylation of choline, ethanolamine, and homoserine serves as precursors to the synthesis of important biological compounds, such as the major phospholipids, phosphatidylcholine and phosphatidylethanolamine and the amino acids, threonine, methionine, and isoleucine. The APH/ChoK-like subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K). Pssm-ID: 270704 [Multi-domain] Cd Length: 234 Bit Score: 71.11 E-value: 1.76e-14
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Name | Accession | Description | Interval | E-value | ||||
YcbJ | COG3173 | Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction ... |
7-234 | 2.99e-22 | ||||
Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction only]; Pssm-ID: 442406 [Multi-domain] Cd Length: 284 Bit Score: 93.64 E-value: 2.99e-22
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APH | pfam01636 | Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance ... |
40-252 | 5.78e-22 | ||||
Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance proteins, which confer resistance to various aminoglycosides they include: aminoglycoside 3'-phosphotransferase or kanamycin kinase / neomycin-kanamycin phosphotransferase and streptomycin 3''-kinase or streptomycin 3''-phosphotransferase. The aminoglycoside phosphotransferases inactivate aminoglycoside antibiotics via phosphorylation. This family also includes homoserine kinase. This family is related to fructosamine kinase pfam03881. Pssm-ID: 426359 [Multi-domain] Cd Length: 239 Bit Score: 92.18 E-value: 5.78e-22
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APH_ChoK_like_1 | cd05155 | Uncharacterized bacterial proteins with similarity to Aminoglycoside 3'-phosphotransferase and ... |
42-226 | 1.76e-14 | ||||
Uncharacterized bacterial proteins with similarity to Aminoglycoside 3'-phosphotransferase and Choline kinase; This subfamily is composed of uncharacterized bacterial proteins with similarity to APH and ChoK. Other APH/ChoK-like proteins include ethanolamine kinase (ETNK), macrolide 2'-phosphotransferase (MPH2'), an unusual homoserine kinase, and uncharacterized proteins with similarity to the N-terminal domain of acyl-CoA dehydrogenase 10 (ACAD10). These proteins catalyze the transfer of the gamma-phosphoryl group from ATP (or CTP) to small molecule substrates, such as aminoglycosides, macrolides, choline, ethanolamine, and homoserine. Phosphorylation of the antibiotics, aminoglycosides, and macrolides leads to their inactivation and to bacterial antibiotic resistance. Phosphorylation of choline, ethanolamine, and homoserine serves as precursors to the synthesis of important biological compounds, such as the major phospholipids, phosphatidylcholine and phosphatidylethanolamine and the amino acids, threonine, methionine, and isoleucine. The APH/ChoK-like subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K). Pssm-ID: 270704 [Multi-domain] Cd Length: 234 Bit Score: 71.11 E-value: 1.76e-14
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MPH2' | cd05152 | Macrolide 2'-Phosphotransferase; MPH2' catalyzes the transfer of the gamma-phosphoryl group ... |
40-254 | 2.38e-13 | ||||
Macrolide 2'-Phosphotransferase; MPH2' catalyzes the transfer of the gamma-phosphoryl group from ATP to the 2'-hydroxyl of macrolide antibiotics such as erythromycin, clarithromycin, and azithromycin, among others. Macrolides penetrate the bacterial cell and bind to ribosomes, where it interrupts protein elongation, leading ultimately to the demise of the bacterium. Phosphorylation of macrolides leads to their inactivation. Based on substrate specificity and amino acid sequence, MPH2' is divided into types I and II, encoded by mphA and mphB genes, respectively. MPH2'I inactivates 14-membered ring macrolides while MPH2'II inactivates both 14- and 16-membered ring macrolides. Enzymatic inactivation of macrolides has been reported as a mechanism for bacterial resistance in clinical samples. MPH2' is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K). Pssm-ID: 270701 [Multi-domain] Cd Length: 276 Bit Score: 68.81 E-value: 2.38e-13
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APH_ChoK_like | cd05120 | Aminoglycoside 3'-phosphotransferase and Choline Kinase family; This family is composed of APH, ... |
40-239 | 3.37e-12 | ||||
Aminoglycoside 3'-phosphotransferase and Choline Kinase family; This family is composed of APH, ChoK, ethanolamine kinase (ETNK), macrolide 2'-phosphotransferase (MPH2'), an unusual homoserine kinase, and uncharacterized proteins with similarity to the N-terminal domain of acyl-CoA dehydrogenase 10 (ACAD10). The members of this family catalyze the transfer of the gamma-phosphoryl group from ATP (or CTP) to small molecule substrates such as aminoglycosides, macrolides, choline, ethanolamine, and homoserine. Phosphorylation of the antibiotics, aminoglycosides and macrolides, leads to their inactivation and to bacterial antibiotic resistance. Phosphorylation of choline, ethanolamine, and homoserine serves as precursors to the synthesis of important biological compounds, such as the major phospholipids, phosphatidylcholine and phosphatidylethanolamine and the amino acids, threonine, methionine, and isoleucine. The APH/ChoK family is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K). Pssm-ID: 270690 [Multi-domain] Cd Length: 158 Bit Score: 63.09 E-value: 3.37e-12
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CotS | COG0510 | Thiamine kinase or a related kinase [Coenzyme transport and metabolism]; |
191-234 | 1.26e-03 | ||||
Thiamine kinase or a related kinase [Coenzyme transport and metabolism]; Pssm-ID: 440276 [Multi-domain] Cd Length: 156 Bit Score: 38.61 E-value: 1.26e-03
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HomoserineK_II | cd05153 | Type II Homoserine Kinase; This subfamily is composed of unusual homoserine kinases, from a ... |
120-221 | 1.28e-03 | ||||
Type II Homoserine Kinase; This subfamily is composed of unusual homoserine kinases, from a subset of bacteria, which have a Protein Kinase fold. These proteins do not bear any similarity to the GHMP family homoserine kinases present in most bacteria and eukaryotes. Homoserine kinase catalyzes the transfer of the gamma-phosphoryl group from ATP to L-homoserine producing L-homoserine phosphate, an intermediate in the production of the amino acids threonine, methionine, and isoleucine. The Type II homoserine kinase subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K). Pssm-ID: 270702 [Multi-domain] Cd Length: 300 Bit Score: 39.93 E-value: 1.28e-03
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SrkA | COG2334 | Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist [Signal ... |
193-221 | 2.04e-03 | ||||
Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist [Signal transduction mechanisms]; Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist is part of the Pathway/BioSystem: Threonine biosynthesis Pssm-ID: 441905 [Multi-domain] Cd Length: 297 Bit Score: 39.14 E-value: 2.04e-03
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