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Conserved domains on  [gi|269789420|gb|ACZ41561|]
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transcriptional activator, TenA family [Thermobaculum terrenum ATCC BAA-798]

Protein Classification

TenA family protein( domain architecture ID 14443674)

TenA family protein such as Bacillus halodurans aminopyrimidine aminohydrolase, which catalyzes an amino-pyrimidine hydrolysis reaction at the C5' of the pyrimidine moiety of thiamine compounds, as part of the thiamine salvage pathway

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TenA_C_BhTenA-like cd19366
TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to ...
 12-223 8.82e-115

TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus halodurans TenA which participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


:

Pssm-ID: 381701 [Multi-domain]  Cd Length: 213  Bit Score: 326.82  E-value: 8.82e-115
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  12 SIHLYELARPIWQKQFEHPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAHMERFGELLLNTIRVEQ 91
Cdd:cd19366    2 SERLREKAKPIWEAGLEHPFVQGLGDGTLDKEKFKFYLKQDYLYLIDYARVFALGAAKADDLETMGRFAELLHGTLNTEM 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  92 ELHRSYAQRFGMSPEEMLSVEMAPTNYAYTSHLHAVASVGTLPEIITAILPCAWIYAEVGKYFSSQGTPASDHPYRDWLL 171
Cdd:cd19366   82 DLHRQYAAEFGITEEELEATEPSPTTLAYTSYMLRTAQTGTLAELLAALLPCAWGYAEIGKRLAEQGGALEHNPYREWIE 161

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 269789420 172 TYSSDDFEQVGAWLRQVLDESTIHADDMEISRLEKIFITSSRYEWMFWDMAW 223
Cdd:cd19366  162 MYSSDEFTELADWLIDLLDELAEGKSEAELERLEEIFLTSSRYEYMFWDMAY 213
 
Name Accession Description Interval E-value
TenA_C_BhTenA-like cd19366
TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to ...
 12-223 8.82e-115

TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus halodurans TenA which participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381701 [Multi-domain]  Cd Length: 213  Bit Score: 326.82  E-value: 8.82e-115
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  12 SIHLYELARPIWQKQFEHPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAHMERFGELLLNTIRVEQ 91
Cdd:cd19366    2 SERLREKAKPIWEAGLEHPFVQGLGDGTLDKEKFKFYLKQDYLYLIDYARVFALGAAKADDLETMGRFAELLHGTLNTEM 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  92 ELHRSYAQRFGMSPEEMLSVEMAPTNYAYTSHLHAVASVGTLPEIITAILPCAWIYAEVGKYFSSQGTPASDHPYRDWLL 171
Cdd:cd19366   82 DLHRQYAAEFGITEEELEATEPSPTTLAYTSYMLRTAQTGTLAELLAALLPCAWGYAEIGKRLAEQGGALEHNPYREWIE 161

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 269789420 172 TYSSDDFEQVGAWLRQVLDESTIHADDMEISRLEKIFITSSRYEWMFWDMAW 223
Cdd:cd19366  162 MYSSDEFTELADWLIDLLDELAEGKSEAELERLEEIFLTSSRYEYMFWDMAY 213
TenA COG0819
Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and ...
 14-227 5.04e-91

Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and metabolism];


Pssm-ID: 440581 [Multi-domain]  Cd Length: 218  Bit Score: 266.75  E-value: 5.04e-91
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  14 HLYELARPIWQKQFEHPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAHMERFGELLLNTIRVEQEL 93
Cdd:COG0819    6 RLREAAAPIWEAILEHPFVQGLADGTLPREAFRHYLVQDYLYLEHFARALALAAAKAPDLEDMRFLAGLAAGLLEVELAL 85

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  94 HRSYAQRFGMSPEEMLSVEMAPTNYAYTSHLHAVASVGTLPEIITAILPCAWIYAEVGKYFSSQGTPAsDHPYRDWLLTY 173
Cdd:COG0819   86 HERYAAELGISEEELEATPPSPTTRAYTSYLLAAAASGDYAELLAALLPCEWGYAEIGKRLAERPLPP-DHPYAEWIETY 164

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....
gi 269789420 174 SSDDFEQVGAWLRQVLDESTIHADDMEISRLEKIFITSSRYEWMFWDMAWRQEQ 227
Cdd:COG0819  165 ASEEFQELVEWLIALLDRLAATASEAERERLEEAFRTASRLEYAFWDMAYRLER 218
salvage_TenA TIGR04306
thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the ...
 19-224 2.97e-52

thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the C-terminal region of trifunctional protein Thi20p from Saccharomyces cerevisiae, perform cleavages on thiamine and related compounds to produce 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP), a substrate a salvage pathway for thiamine biosynthesis. The gene symbol tenA, for Transcription ENhancement A, reflects a misleading early characterization as a regulatory protein. This family is related to PqqC from the PQQ biosynthesis system (see TIGR02111), heme oxygenase (pfam01126), and CADD (Chlamydia protein Associating with Death Domains), a putative folate metabolism enzyme (see TIGR04305).


Pssm-ID: 213919 [Multi-domain]  Cd Length: 208  Bit Score: 167.75  E-value: 2.97e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420   19 ARPIWQKQFEHPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAHMERFGELLLNTIRVEQELHRSYA 98
Cdd:TIGR04306   1 VKPIWDDYINHPFVQKIGDGTLERDKFRFYIEQDYAYLVEYAKVHALGGSKACDEDMEKELVEQIQGGVETEMGQHKRLA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420   99 QRFGMSPEEML-SVEMAPTNYAYTSHLHAVASVGTLPEIITAILPCAWIYAEVGKYFSSQGTpASDHP-YRDWLLTYSSD 176
Cdd:TIGR04306  81 EVLGISDEEYFqKIKPGPALRSYTSYMYDVSRRGNWQELVAALLPCLWGYGEAATKLKGKHT-APEHSvYHKWIETYSSS 159

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*...
gi 269789420  177 DFEQVGAWLRQVLDESTIHADDMEISRLEKIFITSSRYEWMFWDMAWR 224
Cdd:TIGR04306 160 WFREAVNEGENLLNHLAETSSPEELEKLKTIFAESCEYEYNFWTMAYE 207
PRK14713 PRK14713
bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;
15-224 1.24e-47

bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;


Pssm-ID: 237797 [Multi-domain]  Cd Length: 530  Bit Score: 164.19  E-value: 1.24e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  15 LYELARPIWQKQFEHPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAHMERFGELLLNTIRVEQELH 94
Cdd:PRK14713 324 LWQASGPIREAIEDLPFVRALADGTLPEEAFEFYLAQDALYLNGYSRALARLAALAPDPAEQVFWAQSAQACLEVESELH 403

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  95 RSYAQRfgmspeEMLSVEMAPTNYAYTSHLHAVASVGTLPEIITAILPCAWIYAEVGKYFSSQGTPASDHPYRDWLLTYS 174
Cdd:PRK14713 404 RSWLGD------RDADTAPSPVTLAYTDFLLARAAGGSYAVGAAAVLPCFWLYAEVGAELHARAGNPDDHPYAEWLQTYA 477

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|
gi 269789420 175 SDDFEQVGAWLRQVLDESTIHADDMEISRLEKIFITSSRYEWMFWDMAWR 224
Cdd:PRK14713 478 DPEFAAATRRAIAFVDRAFRAASPAERAAMARAFLTACRYELEFFDQARR 527
TENA_THI-4 pfam03070
TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: ...
 19-224 9.38e-43

TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: archaebacteria, eubacteria, and eukaryotes. In Bacillus subtilis, TENA is one of a number of proteins that enhance the expression of extracellular enzymes, such as alkaline protease, neutral protease and levansucrase. The THI-4 protein, which is involved in thiamine biosynthesis, is also a member of this family. The C-terminal part of these proteins consistently show significant sequence similarity to TENA proteins. This similarity was first noted with the Neurospora crassa THI-4. This family includes bacterial coenzyme PQQ synthesis protein C or PQQC proteins. Pyrroloquinoline quinone (PQQ) is the prosthetic group of several bacterial enzymes,including methanol dehydrogenase of methylotrophs and the glucose dehydrogenase of a number of bacteria. PQQC has been found to be required in the synthesis of PQQ but its function is unclear. The exact molecular function of members of this family is uncertain.


Pssm-ID: 397272 [Multi-domain]  Cd Length: 210  Bit Score: 143.65  E-value: 9.38e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420   19 ARPIWQKQFEHPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAHMERFGELLLNTIRVEQELHRSYA 98
Cdd:pfam03070   4 LRPIWKFYINHPFVQALAKGTLPREQFQGYVIQDYLYLVNFARVLAIIASKSPDLEVRREWENRIVDHDGNEIELHLRLA 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420   99 QRFGMSPEEMLSVEMAPTNYAYTSHLHAVASVGTLPEIITAILPCAWIYAEVGKYFSSQGTPASDHPYRDWLLTYSSDDF 178
Cdd:pfam03070  84 EALGLSREDLSAYKPLPATRAYVRYLLDFARRGSWLEAVAALLPCLFVYQEIASRLGEKIRALEGPEYYEWVKTYASEYF 163

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 269789420  179 EQVGAWLRQVLDESTIHADDMEISRLEKIFITSSRYEWMFWDMAWR 224
Cdd:pfam03070 164 RAAVEEAKRLLDRLLAYVSDEQLEELQEIFLKALEFEYSFWTMALD 209
 
Name Accession Description Interval E-value
TenA_C_BhTenA-like cd19366
TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to ...
 12-223 8.82e-115

TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus halodurans TenA which participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381701 [Multi-domain]  Cd Length: 213  Bit Score: 326.82  E-value: 8.82e-115
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  12 SIHLYELARPIWQKQFEHPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAHMERFGELLLNTIRVEQ 91
Cdd:cd19366    2 SERLREKAKPIWEAGLEHPFVQGLGDGTLDKEKFKFYLKQDYLYLIDYARVFALGAAKADDLETMGRFAELLHGTLNTEM 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  92 ELHRSYAQRFGMSPEEMLSVEMAPTNYAYTSHLHAVASVGTLPEIITAILPCAWIYAEVGKYFSSQGTPASDHPYRDWLL 171
Cdd:cd19366   82 DLHRQYAAEFGITEEELEATEPSPTTLAYTSYMLRTAQTGTLAELLAALLPCAWGYAEIGKRLAEQGGALEHNPYREWIE 161

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 269789420 172 TYSSDDFEQVGAWLRQVLDESTIHADDMEISRLEKIFITSSRYEWMFWDMAW 223
Cdd:cd19366  162 MYSSDEFTELADWLIDLLDELAEGKSEAELERLEEIFLTSSRYEYMFWDMAY 213
TenA COG0819
Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and ...
 14-227 5.04e-91

Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and metabolism];


Pssm-ID: 440581 [Multi-domain]  Cd Length: 218  Bit Score: 266.75  E-value: 5.04e-91
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  14 HLYELARPIWQKQFEHPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAHMERFGELLLNTIRVEQEL 93
Cdd:COG0819    6 RLREAAAPIWEAILEHPFVQGLADGTLPREAFRHYLVQDYLYLEHFARALALAAAKAPDLEDMRFLAGLAAGLLEVELAL 85

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  94 HRSYAQRFGMSPEEMLSVEMAPTNYAYTSHLHAVASVGTLPEIITAILPCAWIYAEVGKYFSSQGTPAsDHPYRDWLLTY 173
Cdd:COG0819   86 HERYAAELGISEEELEATPPSPTTRAYTSYLLAAAASGDYAELLAALLPCEWGYAEIGKRLAERPLPP-DHPYAEWIETY 164

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....
gi 269789420 174 SSDDFEQVGAWLRQVLDESTIHADDMEISRLEKIFITSSRYEWMFWDMAWRQEQ 227
Cdd:COG0819  165 ASEEFQELVEWLIALLDRLAATASEAERERLEEAFRTASRLEYAFWDMAYRLER 218
TenA_C-like cd19365
uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as ...
 12-222 1.69e-71

uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA_C- like proteins.


Pssm-ID: 381700 [Multi-domain]  Cd Length: 205  Bit Score: 216.61  E-value: 1.69e-71
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  12 SIHLYELARPIWQKQFEHPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAHMERFGELLLNTIRVEQ 91
Cdd:cd19365    1 TEALWEAIAPIYAAILAHPFIRELADGTLPREKFRFYLAQDALYLRDYARALALLAARAPDPEEQVFFARFAAGAIEVER 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  92 ELHRSYAQRFGMSPEemlsVEMAPTNYAYTSHLHAVASVGTLPEIITAILPCAWIYAEVGKYFSSQGTPasDHPYRDWLL 171
Cdd:cd19365   81 ELHRSFLGEFGIDAA----AEPSPVTLAYTSFLLATAATGPYAVAVAAVLPCFWIYAEVGKRLAAAASP--NHPYQDWID 154

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|.
gi 269789420 172 TYSSDDFEQVGAWLRQVLDESTIHADDMEISRLEKIFITSSRYEWMFWDMA 222
Cdd:cd19365  155 TYSDEEFAEAVRRAIAIVDRLAAEASPEERARMLEAFLRASRLEWMFWDAA 205
TenA_C_BsTenA-like cd19364
TenA_C proteins similar to Bacillus subtilis TenA; This family of TenA proteins belongs to the ...
 12-224 2.80e-67

TenA_C proteins similar to Bacillus subtilis TenA; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus subtilis TenA which has been shown to be a thiaminase II, catalyzing the hydrolysis of thiamine into HMP and 5-(2-hydroxyethyl)-4-methylthiazole (THZ), within thiamine metabolism. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381699 [Multi-domain]  Cd Length: 212  Bit Score: 206.27  E-value: 2.80e-67
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  12 SIHLYELARPIWQKQFEHPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAHMERFGELLLNTIRVEQ 91
Cdd:cd19364    2 TEELREAADPLWQKSFEHPFIQGLADGTLPLETFRYYLIQDAYYLKHFAKLHALAAAKADDPAIKALLLEGAQGLAEGEL 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  92 ELHRSYAQRFGMSPEEMLSVEMAPTNYAYTSHLHAVASVGTLPEIITAILPCAWIYAEVGKYFSSQGtpaSDHP-YRDWL 170
Cdd:cd19364   82 ALRETFFKELGITEEEIAQTPPAPTAYHYVSHMYRQLNEGSVAEAVAALLPCYWLYQEIGERLADAG---SPVPlYQRWI 158

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....
gi 269789420 171 LTYSSDDFEQVGAWLRQVLDESTIHADDMEISRLEKIFITSSRYEWMFWDMAWR 224
Cdd:cd19364  159 DTYASDEFTESVQQQIDLVDRLAEEASEEEREKMKQAFLISSYYELQFWEMAYT 212
TenA_PqqC-like cd16099
TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone ...
 22-221 8.84e-67

TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone (PQQ) synthesis protein C; TenA proteins participate in thiamin metabolism and can be classified into two classes: TenA_C which has an active site Cys, and TenA_E which does not; TenA_E proteins often have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product amino-HMP (4-amino-5-amino-methyl-2-methylpyrimidine) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP, but does not hydrolyze thiamin. Bacillus subtilis TenA_C can hydrolyze amino-HMP to AMP and can catalyze the hydrolysis of thiamin. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal ThiD domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). Bacillus halodurans TenA_C participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. Helicobacter pylori TenA_C is also thought to catalyze a salvage reaction but the pyrimidine substrate has not yet been identified. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect; Pyrococcus furiosus TenA_E lacks appropriate surface charges for DNA interactions. This family also includes bacterial coenzyme pyrroloquinoline quinone (PQQ) synthesis protein C (PQQC), an oxidase involved in the final step of PQQ biosynthesis, and CADD, a Chlamydia protein that interacts with death receptors.


Pssm-ID: 381691 [Multi-domain]  Cd Length: 196  Bit Score: 204.51  E-value: 8.84e-67
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  22 IWQKQFEHPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAHMERFgELLLNTIRVEQELHRSYAQRF 101
Cdd:cd16099    1 IWDAILNHPFVQELAAGTLPREKFRYYLAQDYYYLKDFARALALAAAKAPDLELRTFL-AELINVLDDELELHEKLLAEL 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420 102 GMSPEEMLSVEMAPTNYAYTSHLHAVASVGTLPEIITAILPCAWIYAEVGKYFSSQGTPasDHPYRDWLLTYSSDDFEQV 181
Cdd:cd16099   80 GISEEDLSEAEPNPATLAYTNHLLRVAARGTPAEGLAALLPCYWSYGEIGRRLAASLPE--HPPYRFWIDFYASDEYQEL 157

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|
gi 269789420 182 GAWLRQVLDESTiHADDMEISRLEKIFITSSRYEWMFWDM 221
Cdd:cd16099  158 VEELLQLLDQLA-AAGEEEKEELKEIFLTSLRYELMFWDA 196
TenA_C_HP1287-like cd19361
TenA_C proteins similar to Helicobacter pylori TenA (HP1287; This family of TenA proteins ...
 15-221 1.76e-62

TenA_C proteins similar to Helicobacter pylori TenA (HP1287; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Helicobacter pylori TenA (HP1287) protein which is thought to catalyze a salvage reaction in thiamin metabolism, however its pyrimidine substrate has not yet been identified. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. HP1287 may contribute to stomach colonization and persistence.


Pssm-ID: 381696 [Multi-domain]  Cd Length: 212  Bit Score: 193.95  E-value: 1.76e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  15 LYELARPIWQKQFEHPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAHMERFGELLLNTIRVEQELH 94
Cdd:cd19361    5 LYEAVEDIWDSYYEHPFVQGIADGTLDIEKFRFYMIQDYLYLLDYAKVFALGVAKAKDEEVMRFFADLINAILNEEMDIH 84

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  95 RSYAQRFGMSPEEMLSVEMAPTNYAYTSHLHAVASVGTLPEIITAILPCAWIYAEVGKYFSSQGTPASDHP-YRDWLLTY 173
Cdd:cd19361   85 RGYMKRLGITEEEIENTKPALDNLSYTSYMLSVAYEGGIAEILAAILSCSWSYEYIAKKLVERYPAALEHEfYGEWVKGY 164

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*...
gi 269789420 174 SSDDFEQVGAWLRQVLDESTIHADDMEISRLEKIFITSSRYEWMFWDM 221
Cdd:cd19361  165 SSEEYAEANQELIDLLDRLTEDISEEQIEKLEEIFVNCSRYELKFWDM 212
TenA_C_SaTenA-like cd19360
TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins ...
 15-221 2.06e-56

TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Staphylococcus aureus TenA (SaTenA) which plays two essential roles in thiamin metabolism: in the deamination of aminopyrimidine to HMP, and in hydrolyzing thiamin into HMP and 5-(2-hydroxyethyl)4-methylthiazole (THZ). It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. SaTenA is then also a putative transcriptional regulator controlling the secretion of extracellular proteases such as subtilisin-type proteases in bacteria. This family includes mostly uncharacterized TenA like proteins.


Pssm-ID: 381695 [Multi-domain]  Cd Length: 211  Bit Score: 178.55  E-value: 2.06e-56
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  15 LYELARPIWQKQFEHPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAHMERFGELLLNTIRVEQELH 94
Cdd:cd19360    5 LREEAQPILEAIYAHPFVQGIAAGELPKEALIHYVQQDYEYLNAFLKVYALAIAKSDTREDMRFFLEQIGFILNGESHAH 84

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  95 RSYAQRFGMSPEEMLSVEMAPTNYAYTSHLHAVASVGTLPEIITAILPCAWIYAEVGKYFSSQGTPASDHPYRDWLLTYS 174
Cdd:cd19360   85 QNLCEVAGVDYEELQGAPWAPTADHYIKHMYYAARTGDLGDILAALLPCPWTYVELAKRLIEEGKPTPDNPFYEWIDFYA 164

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*..
gi 269789420 175 SDDFEQVGAWLRQVLDESTIHADDMEISRLEKIFITSSRYEWMFWDM 221
Cdd:cd19360  165 DDEMDGLTDQLFARLDRLAEKASEEERERAKQAFLKSCQLEWRFWEM 211
TenA_C-like cd19369
uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as ...
 21-221 3.18e-54

uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA_C- like proteins.


Pssm-ID: 381704 [Multi-domain]  Cd Length: 202  Bit Score: 172.80  E-value: 3.18e-54
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  21 PIWQKQFEHPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAHMERFGELLLNTIRVEQELHRSYAQR 100
Cdd:cd19369    1 PIWEQYLEHPFIKELGEGTLDKEKFKNYLIQDSLYLKEYAKVFAMGIYKSRTMKEMQFFYSSLSFVNEDETATRIKYLKE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420 101 FGMSPEEMLSVEMAPTNYAYTSHLHAVASVGTLPEIITAILPCAWIYAEVGKYFSSQGTP-ASDHPYRDWLLTYSSDDFE 179
Cdd:cd19369   81 FGLTDEDIEKIEPLPENKAYTDYMLGIAKTGDVKEILMAVLPCMLSYYYIFKELVKKYKDnLESNPYKDWIEDYASEEYA 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|..
gi 269789420 180 QVGAWLRQVLDESTIHADDMEISRLEKIFITSSRYEWMFWDM 221
Cdd:cd19369  161 EYCKEWIDFADRLCENLSEEEKEKLKEIFRKASLYELKFWDM 202
TenA_C_PH1161-like cd19363
uncharacterized TenA_C proteins similar to Pyrococcus horikoshii PH1161; This family of TenA ...
 14-223 8.40e-54

uncharacterized TenA_C proteins similar to Pyrococcus horikoshii PH1161; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes functionally uncharacterized TenA like proteins such as Pyrococcus horikoshii PH1161 protein.


Pssm-ID: 381698 [Multi-domain]  Cd Length: 210  Bit Score: 171.74  E-value: 8.40e-54
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  14 HLYELARPIWQKQFEHPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAHMERFGELLLNTIRVEQEL 93
Cdd:cd19363    4 KLRRDADDIWKKILNHPFVVELYSGTLPMEKFKFYLLQDYNYLVGSTKNLSILASKAESLDLMRELLELAYGEATTEFAN 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  94 HRSYAQRFGMSPEEMLSVEMAPTNYAYTSHLHAVASVGTLPEIITAILPCAWIYAEVGKYFSSQGTPASDHPYRDWLLTY 173
Cdd:cd19363   84 YEELLDELGLSLEDAIKVEPFPTNVAYMNFLLSTSSLGSFYEGLAALLPCFWSYLEIAEYHKDKLSENPNDIYRDWASVY 163

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|
gi 269789420 174 SSDDFEQVGAWLRQVLDEStihADDMEISRLEKIFITSSRYEWMFWDMAW 223
Cdd:cd19363  164 LSKEYKELVERLRRIVDKY---GEGEPFEKLKRIFKTASKYEYMFWDAAY 210
salvage_TenA TIGR04306
thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the ...
 19-224 2.97e-52

thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the C-terminal region of trifunctional protein Thi20p from Saccharomyces cerevisiae, perform cleavages on thiamine and related compounds to produce 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP), a substrate a salvage pathway for thiamine biosynthesis. The gene symbol tenA, for Transcription ENhancement A, reflects a misleading early characterization as a regulatory protein. This family is related to PqqC from the PQQ biosynthesis system (see TIGR02111), heme oxygenase (pfam01126), and CADD (Chlamydia protein Associating with Death Domains), a putative folate metabolism enzyme (see TIGR04305).


Pssm-ID: 213919 [Multi-domain]  Cd Length: 208  Bit Score: 167.75  E-value: 2.97e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420   19 ARPIWQKQFEHPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAHMERFGELLLNTIRVEQELHRSYA 98
Cdd:TIGR04306   1 VKPIWDDYINHPFVQKIGDGTLERDKFRFYIEQDYAYLVEYAKVHALGGSKACDEDMEKELVEQIQGGVETEMGQHKRLA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420   99 QRFGMSPEEML-SVEMAPTNYAYTSHLHAVASVGTLPEIITAILPCAWIYAEVGKYFSSQGTpASDHP-YRDWLLTYSSD 176
Cdd:TIGR04306  81 EVLGISDEEYFqKIKPGPALRSYTSYMYDVSRRGNWQELVAALLPCLWGYGEAATKLKGKHT-APEHSvYHKWIETYSSS 159

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*...
gi 269789420  177 DFEQVGAWLRQVLDESTIHADDMEISRLEKIFITSSRYEWMFWDMAWR 224
Cdd:TIGR04306 160 WFREAVNEGENLLNHLAETSSPEELEKLKTIFAESCEYEYNFWTMAYE 207
TenA_C_ScTHI20-like cd19367
TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 ...
 21-223 1.20e-49

TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 protein; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal HMP kinase/HMP-P kinase (ThiD-like) domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). A majority of this family are single-domain TenA_C- like proteins; some however have additional domains such as a ThiD domain. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381702 [Multi-domain]  Cd Length: 204  Bit Score: 161.14  E-value: 1.20e-49
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  21 PIWQKQFEHPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAHMERFGELLLNtIRVEQELHRSYAQR 100
Cdd:cd19367    1 PIWKAYTNHPFVRQLADGTLPLESFRHYLKQDYLYLIHYARAHALAAYKAPTLEDIRAAAAILLA-ILEEMELHVKYCAE 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420 101 FGMSPEEMLSVEMAPTNYAYTSHLHAVASVGTLPEIITAILPCAWIYAEVGKYFSSQ-GTPASDHPYRDWLLTYSSDDFE 179
Cdd:cd19367   80 WGISEEELEATPESPATLAYTRYVLDVGLSGDLLDLLVALAPCLLGYGEIGLRLAADpATKLEGNPYWSWIETYASDEYQ 159

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*...
gi 269789420 180 QVGAWLRQVLDEstiHADDM----EISRLEKIFITSSRYEWMFWDMAW 223
Cdd:cd19367  160 EAVREGIELLEK---LAAERpspaRLEELVKIFATATRLEIGFWDMGL 204
PRK14713 PRK14713
bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;
15-224 1.24e-47

bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;


Pssm-ID: 237797 [Multi-domain]  Cd Length: 530  Bit Score: 164.19  E-value: 1.24e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  15 LYELARPIWQKQFEHPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAHMERFGELLLNTIRVEQELH 94
Cdd:PRK14713 324 LWQASGPIREAIEDLPFVRALADGTLPEEAFEFYLAQDALYLNGYSRALARLAALAPDPAEQVFWAQSAQACLEVESELH 403

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  95 RSYAQRfgmspeEMLSVEMAPTNYAYTSHLHAVASVGTLPEIITAILPCAWIYAEVGKYFSSQGTPASDHPYRDWLLTYS 174
Cdd:PRK14713 404 RSWLGD------RDADTAPSPVTLAYTDFLLARAAGGSYAVGAAAVLPCFWLYAEVGAELHARAGNPDDHPYAEWLQTYA 477

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|
gi 269789420 175 SDDFEQVGAWLRQVLDESTIHADDMEISRLEKIFITSSRYEWMFWDMAWR 224
Cdd:PRK14713 478 DPEFAAATRRAIAFVDRAFRAASPAERAAMARAFLTACRYELEFFDQARR 527
TENA_THI-4 pfam03070
TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: ...
 19-224 9.38e-43

TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: archaebacteria, eubacteria, and eukaryotes. In Bacillus subtilis, TENA is one of a number of proteins that enhance the expression of extracellular enzymes, such as alkaline protease, neutral protease and levansucrase. The THI-4 protein, which is involved in thiamine biosynthesis, is also a member of this family. The C-terminal part of these proteins consistently show significant sequence similarity to TENA proteins. This similarity was first noted with the Neurospora crassa THI-4. This family includes bacterial coenzyme PQQ synthesis protein C or PQQC proteins. Pyrroloquinoline quinone (PQQ) is the prosthetic group of several bacterial enzymes,including methanol dehydrogenase of methylotrophs and the glucose dehydrogenase of a number of bacteria. PQQC has been found to be required in the synthesis of PQQ but its function is unclear. The exact molecular function of members of this family is uncertain.


Pssm-ID: 397272 [Multi-domain]  Cd Length: 210  Bit Score: 143.65  E-value: 9.38e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420   19 ARPIWQKQFEHPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAHMERFGELLLNTIRVEQELHRSYA 98
Cdd:pfam03070   4 LRPIWKFYINHPFVQALAKGTLPREQFQGYVIQDYLYLVNFARVLAIIASKSPDLEVRREWENRIVDHDGNEIELHLRLA 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420   99 QRFGMSPEEMLSVEMAPTNYAYTSHLHAVASVGTLPEIITAILPCAWIYAEVGKYFSSQGTPASDHPYRDWLLTYSSDDF 178
Cdd:pfam03070  84 EALGLSREDLSAYKPLPATRAYVRYLLDFARRGSWLEAVAALLPCLFVYQEIASRLGEKIRALEGPEYYEWVKTYASEYF 163

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 269789420  179 EQVGAWLRQVLDESTIHADDMEISRLEKIFITSSRYEWMFWDMAWR 224
Cdd:pfam03070 164 RAAVEEAKRLLDRLLAYVSDEQLEELQEIFLKALEFEYSFWTMALD 209
TenA_C_AtTH2-like cd19368
TenA_C family similar to the N-terminal TenA_C domain of Arabidopsis thaliana thiamine ...
 18-208 2.78e-40

TenA_C family similar to the N-terminal TenA_C domain of Arabidopsis thaliana thiamine requiring 2; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Arabidopsis thaliana TH2 is an orphan enzyme thiamin monophosphate phosphatase which has a haloacid dehalogenase (HAD) family domain fused to its TenA_C domain, it's TenA_C domain has thiamin salvage hydrolase activity against amino-HMP. This family includes mostly uncharacterized single-domain TenA_C- like proteins; some however have additional domains such as a HAD family domain or a kinase domain It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381703 [Multi-domain]  Cd Length: 210  Bit Score: 137.37  E-value: 2.78e-40
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  18 LARPIWQK-----QF--EHPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAHMERFGELLLNTIRvE 90
Cdd:cd19368    1 LAKRLWKKnrdeaLFalYHPFVVGLAAGNLPLDSFRHYISQDAHFLEAFARAYELAEAKADDDEDKKAIRELRKGVLE-E 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  91 QELHRSYAQRFGMSPEEmlSVEMAPTNYAYTSHLHAVAS--VGTLPEIITAILPCAWIYAEVGKYFSSQGTPASDHPYRD 168
Cdd:cd19368   80 LKLHDSYAEEWGVDLPK--EVTPDPATRKYTDFLLATASgkVKVAAYTLAAMAPCMRLYAFLGQELARALDDTEDHPYKK 157

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|
gi 269789420 169 WLLTYSSDDFEQVGAWLRQVLDESTIHADDMEISRLEKIF 208
Cdd:cd19368  158 WIDTYSSQEFEALALQLEDLLDKLSASLTGEELEALEKLY 197
PTZ00347 PTZ00347
phosphomethylpyrimidine kinase; Provisional
 29-230 1.61e-34

phosphomethylpyrimidine kinase; Provisional


Pssm-ID: 240375 [Multi-domain]  Cd Length: 504  Bit Score: 128.54  E-value: 1.61e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  29 HPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAHMerfGELLLNTIRVEQEL---HRSYAQrfgmsp 105
Cdd:PTZ00347  31 LPFVQGLGDGTLDQNAFRTYIAQDTLYLNGYIRILSYCITKSDVTATG---GGLLELLKGVLEELkncHHHYID------ 101

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420 106 eemlSVEMAPTNYA---YTSHLHAVASVGTLP--EIITAILPCAWIYAEVGKYFSSQGTPASDHPYRDWLLTYSSDDFEQ 180
Cdd:PTZ00347 102 ----NPDAAGPEAAcrkYVDFLLASGNADTLGpsVVIAAVIPCARLYAWVGQELTNEVELTESHPFRRWLLSYSDEPINT 177

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|
gi 269789420 181 VGAWLRQVLDEstiHADDMEISRLEKIFITSSRYEWMFWDMAWRQEQWPV 230
Cdd:PTZ00347 178 SVEQLESLLDK---YIRPGEFSEVAQAYRRAMELEYDFFDSFGYCLGRPV 224
TenA_C_SsTenA-1-like cd19362
uncharacterized TenA_C proteins similar to Sulfolobus solfataricus TenA-1 (Sso2206); This ...
 15-222 1.04e-31

uncharacterized TenA_C proteins similar to Sulfolobus solfataricus TenA-1 (Sso2206); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA like proteins such as Sulfolobus solfataricus putative TenA-like thiaminase (Tena-1, Sso2206).


Pssm-ID: 381697 [Multi-domain]  Cd Length: 200  Bit Score: 114.85  E-value: 1.04e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  15 LYELARPIWQKQFEHPFVTSLGDGSLPQENFRFYICQDALFLNELTKifgyATTRSQQLAHMERFGELL---LNTIRVEQ 91
Cdd:cd19362    4 LKNLVGDLWNKYVRHEFVERMRDGTLPLDNFRYYLIQDSKYVEEMLR----ALLRASSKAPLDKAIKILnsvFSGRDKGM 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  92 ELHRSYAQRFGMSPEEMLSVEMAPTNYAYTSHLHAVASVGtLPEIITAILPCAWIYAEVGKYfsSQGTPasDHPYRDWLL 171
Cdd:cd19362   80 EVHKFLYSELGITEDEIRRTGYNLVNYAYTRHLYYYSTLG-WPQFLAAWAPCMWGYSEIGKY--VLNSP--NELYKTWAS 154

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|...
gi 269789420 172 TYSSDDFE-QVGAWLRQVldestihaDDMEISR-LEKIFITSSRYEWMFWDMA 222
Cdd:cd19362  155 FYASKDYKkRVEAILEAL--------DSIEDTEdIKNIFRNSVNFEIMFWDAA 199
PRK09517 PRK09517
multifunctional thiamine-phosphate pyrophosphorylase/synthase/phosphomethylpyrimidine kinase; ...
 30-225 2.83e-29

multifunctional thiamine-phosphate pyrophosphorylase/synthase/phosphomethylpyrimidine kinase; Provisional


Pssm-ID: 169939 [Multi-domain]  Cd Length: 755  Bit Score: 115.07  E-value: 2.83e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  30 PFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAHMERFGELLLNTIRVEQELHRSYaqrfgMSPEEML 109
Cdd:PRK09517 565 DFIRMLGDGTLRRPEFDFYIDQDAQYLRQYSRALARLSSIAPDSHAQVEWAQSAAECIVVEAELHRSY-----LSGKEAP 639

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420 110 SVEmAPTNYAYTSHLHAVASVGTLPEIITAILPCAWIYAEVGKYFSSQGTPAsdHPYRDWLLTYSSDDFEQVGAWLRQVL 189
Cdd:PRK09517 640 SAP-SPVTMAYTDFLIARTYTEDYVVGVAAVLPCYWLYAEIGLMLAEQNHDE--HPYKDWLNTYSGEEFIAGTRAAIARV 716

                        170       180       190
                 ....*....|....*....|....*....|....*.
gi 269789420 190 DESTIHADDMEISRLEKIFITSSRYEWMFWDMAWRQ 225
Cdd:PRK09517 717 EKALENAGPEQRVDAARAFLSASVHEREFFDQATRH 752
TenA_E_Spr0628-like cd19358
TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C ...
 15-223 1.98e-28

TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C PET18; This family of TenA proteins belongs to the TenA_E class, and lacks the conserved active site Cys residue of the TenA_C class; most have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2; aminopyrimidine aminohydrolase, also known as thiaminase II) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E (not belonging to this family) hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP. Members of this family include the putative thiaminase Streptococcus pneumoniae Spr0628, and Saccharomyces cerevisiae S288C PET18, a protein of unknown function whose expression is induced in the absence of thiamin. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. Many proteins in this family have yet to be characterized.


Pssm-ID: 381693 [Multi-domain]  Cd Length: 209  Bit Score: 106.50  E-value: 1.98e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  15 LYELARPIWQKQFEHPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAHMERFGE---LLLNtirvEQ 91
Cdd:cd19358    5 LRAANAEDWDAAVTHRFVRELCAGTLPDAVLARYLVQDYQFVETFLRLLGKAVAKAPDLEAKLRLARflgFLAN----DE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  92 E--LHRSYAQRfGMSPEEMLSVEMAPTNYAYTSHLHAVASVGTLPEIITAILPCAWIYAEVGKYFSSqgtPASDHP-YRD 168
Cdd:cd19358   81 NdyFERAFAAL-GVSEADREAPPLLPATRAFIDLMLEAARSGSYAEILTVLLVAEWLYLDWASRAAA---AAPLRFkHQE 156

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 269789420 169 WLLTYSSDDFEQVGAWLRQVLDESTIHADDMEisRLEKIFITSSRYEWMFWDMAW 223
Cdd:cd19358  157 WIDLHSGPEFEAWVDFLRDEVDRVGPTEEERE--RLEAVFARAVELEIAFFDAAY 209
TenA_E_At3g16990-like cd19357
TenA_E proteins similar to Arabidopsis thaliana At3g16990; This family of TenA proteins ...
 14-221 5.77e-19

TenA_E proteins similar to Arabidopsis thaliana At3g16990; This family of TenA proteins belongs to the TenA_E class, and lacks the conserved active site Cys residue of the TenA_C class; most have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2; aminopyrimidine aminohydrolase, also known as thiaminase II) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Members of this family include Arabidopsis thaliana At3g16990, Zea mays GRMZM2G080501, and Pyrococcus furiosus PF1337, among others. Arabidopsis thaliana TenA_E hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP, but does not hydrolyze thiamin; nor does it have activity with other thiamine degradation products such as thiamine mono- or diphosphate, oxythiamine, oxothiamine, thiamine disulfide, desthiothiamine or thiochrome as substrates. Structural studies of P. furiosus PF1337 strongly support its enzymatic function in thiamine biosynthesis. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381692 [Multi-domain]  Cd Length: 217  Bit Score: 81.60  E-value: 5.77e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  14 HLYELARPIWQKQFEHPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFG--------YATTRSQQLAHMERFGellLN 85
Cdd:cd19357    3 HLLSLHPALYTAATQHPFLRAAADGTLPKEALSRWLAQDRLYVQAYIRFLGsllaraplPSSSLNQRLLDVLLGA---LA 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  86 TIRVEQELHRSYAQRFGMSPEEmLSVEMAPTNYAYTSHLHAVASVG-TLPEIITAIlpcaW----IYAEVGKYFSSQGTP 160
Cdd:cd19357   80 NLRRELAFFEETAAEYGLDLPG-LGVPPSPATRAYVDFLASLASEGvSYLEGLVVL----WatekVYLDAWSYARSFLPS 154

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 269789420 161 ASD--HPYRDWLLTYSSDDFEQVGAWLRQVLDESTIHADDMEISRLEKIFITSSRYEWMFWDM 221
Cdd:cd19357  155 DADggALYREFIPNWTSPEFAAFVDRLGDLVDEALEQAGEEVLERAEEVWRRVLELEEAFWPM 217
TenA_C_Bt3146-like cd19359
uncharacterized TenA_C proteins similar to Bacteroides thetaiotaomicron Bt3146; This family of ...
 22-220 7.93e-10

uncharacterized TenA_C proteins similar to Bacteroides thetaiotaomicron Bt3146; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA-like proteins such as Bacteroides thetaiotaomicron Bt3146.


Pssm-ID: 381694 [Multi-domain]  Cd Length: 206  Bit Score: 56.59  E-value: 7.93e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420  22 IWQKQFEHPFVTSLGDGSLPQENFRFYICQDALFLNELTKIFGYATTRSQQLAhmerfgelLLNTIRVEQELHRSYAQRF 101
Cdd:cd19359   11 LWDKALNNPFCQGMADGTLDLDGFGYYMVQDYYYCINYVRFKALRAAKAPDPD--------LLAFLAAKIKSYLDYAEDF 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269789420 102 -------GMSPEEMLSVEMAPTNYAYTSHLHAVASVGTLPEIITAILPCAWIYAEVGKYFSSQGTPASDHPYRDWLL--T 172
Cdd:cd19359   83 lktchikLGIPDVVDGVKPSPALKAYVDFERSVAESEDWFYLLVAMIPCIYLWYWLANQLNEDPSDKNTNFYKTWIEpnL 162

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*...
gi 269789420 173 YSSDDFEQVGAWLRQVLDESTIHADdmeisRLEKIFITSSRYEWMFWD 220
Cdd:cd19359  163 PDPSSAKQLEFFLNANAAWSKIDRE-----KANEIFRQAMQLEINFFN 205
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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