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Conserved domains on  [gi|256013060|gb|EAL71892|]
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hypothetical protein DDB_G0271120 [Dictyostelium discoideum AX4]

Protein Classification

PRKCSH domain-containing protein( domain architecture ID 719466)

PRKCSH (protein kinase C substrate 80K-H) domain-containing protein similar to Ustilago maydis glucosidase 2 subunit beta, is a subunit of glucosidase 2, which cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins in the endoplasmic reticulum (ER)

CATH:  2.70.130.10

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRKCSH-like super family cl28164
Glucosidase II beta subunit-like; The sequences found in this family are similar to a region ...
 20-172 2.91e-40

Glucosidase II beta subunit-like; The sequences found in this family are similar to a region found in the beta-subunit of glucosidase II, which is also known as protein kinase C substrate 80K-H (PRKCSH). The enzyme catalyzes the sequential removal of two alpha-1,3-linked glucose residues in the second step of N-linked oligosaccharide processing. The beta subunit is required for the solubility and stability of the heterodimeric enzyme, and is involved in retaining the enzyme within the endoplasmic reticulum.


The actual alignment was detected with superfamily member pfam12999:

Pssm-ID: 372423 [Multi-domain]  Cd Length: 176  Bit Score: 143.39  E-value: 2.91e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 256013060   20 SLSPTYGVGPEELEYYK--EGKYFNCL-RSNVQIPFSQVNDDFCDCPDGTDEPGTSACsSNGRFYCQNIGHKGNYISSSF 96
Cdd:pfam12999  15 ALGKLRGVSPDNLHLYQpdENGNWKCLnHSEIKLSFDQVNDDYCDCPDGSDEPGTNAC-SNGKFYCANEGFIPGYIPSFK 93

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 256013060   97 VNDGVCD---CCDGSDEYQlkVKCKNNCKEIGEESRKKQNQVIEAYEIGLKKKKQMEEEGTRvfneKTDEIIRLRKEID 172
Cdd:pfam12999  94 VDDGVCDydiCCDGSDEAL--GKCPNKCGEIARQFEEYLTEHNNSVKNGLKIKEGLLLAAQK----KRDELKKRLKELE 166
PRKCSH super family cl06793
Glucosidase II beta subunit-like protein; The sequences found in this family are similar to a ...
 379-523 3.37e-24

Glucosidase II beta subunit-like protein; The sequences found in this family are similar to a region found in the beta-subunit of glucosidase II, which is also known as protein kinase C substrate 80K-H (PRKCSH). The enzyme catalyzes the sequential removal of two alpha-1,3-linked glucose residues in the second step of N-linked oligosaccharide processing. The beta subunit is required for the solubility and stability of the heterodimeric enzyme, and is involved in retaining the enzyme within the endoplasmic reticulum. Mutations in the gene coding for PRKCSH have been found to be involved in the development of autosomal dominant polycystic liver disease (ADPLD), but the precise role the protein has in the pathogenesis of this disease is unknown. This family also includes an ER sensor for misfolded glycoproteins and is therefore likely to be a generic sugar binding domain.


The actual alignment was detected with superfamily member pfam13015:

Pssm-ID: 414904  Cd Length: 154  Bit Score: 98.75  E-value: 3.37e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 256013060  379 LESELSKKRESLKEKENEIEKIDKMLKSDLGVNNVFIPLYSKCFDLATKEYTYSVCPYEKASQGHTSLGKFESFGDNgkM 458
Cdd:pfam13015   1 LQMSIDEHEKDIKKIESDITILEENLNSRYGPDDILRAYEGRETKEKIGGYTYKVCFLGSIFQDDISIGNFKKQEGN--K 78

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 256013060  459 MLFENGQQCWGGPKRSLKVLMECGQDNELYDVQEPGKCEYTIKFKTPVLCSEEHLKILRLEGDHS 523
Cdd:pfam13015  79 LYYENGAKCWNGPHRSAIVEVECGDVNELVSVSEPEKCEYLFVVKSPAACSNVYLVKSLAEEESL 143
 
Name Accession Description Interval E-value
PRKCSH-like pfam12999
Glucosidase II beta subunit-like; The sequences found in this family are similar to a region ...
 20-172 2.91e-40

Glucosidase II beta subunit-like; The sequences found in this family are similar to a region found in the beta-subunit of glucosidase II, which is also known as protein kinase C substrate 80K-H (PRKCSH). The enzyme catalyzes the sequential removal of two alpha-1,3-linked glucose residues in the second step of N-linked oligosaccharide processing. The beta subunit is required for the solubility and stability of the heterodimeric enzyme, and is involved in retaining the enzyme within the endoplasmic reticulum.


Pssm-ID: 372423 [Multi-domain]  Cd Length: 176  Bit Score: 143.39  E-value: 2.91e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 256013060   20 SLSPTYGVGPEELEYYK--EGKYFNCL-RSNVQIPFSQVNDDFCDCPDGTDEPGTSACsSNGRFYCQNIGHKGNYISSSF 96
Cdd:pfam12999  15 ALGKLRGVSPDNLHLYQpdENGNWKCLnHSEIKLSFDQVNDDYCDCPDGSDEPGTNAC-SNGKFYCANEGFIPGYIPSFK 93

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 256013060   97 VNDGVCD---CCDGSDEYQlkVKCKNNCKEIGEESRKKQNQVIEAYEIGLKKKKQMEEEGTRvfneKTDEIIRLRKEID 172
Cdd:pfam12999  94 VDDGVCDydiCCDGSDEAL--GKCPNKCGEIARQFEEYLTEHNNSVKNGLKIKEGLLLAAQK----KRDELKKRLKELE 166
PRKCSH_1 pfam13015
Glucosidase II beta subunit-like protein; The sequences found in this family are similar to a ...
 379-523 3.37e-24

Glucosidase II beta subunit-like protein; The sequences found in this family are similar to a region found in the beta-subunit of glucosidase II, which is also known as protein kinase C substrate 80K-H (PRKCSH). The enzyme catalyzes the sequential removal of two alpha-1,3-linked glucose residues in the second step of N-linked oligosaccharide processing. The beta subunit is required for the solubility and stability of the heterodimeric enzyme, and is involved in retaining the enzyme within the endoplasmic reticulum. The beta-subunit confers substrate specificity for di- and monoglucosylated glycans on the glucose-trimming activity of the alpha-subunit.


Pssm-ID: 404038  Cd Length: 154  Bit Score: 98.75  E-value: 3.37e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 256013060  379 LESELSKKRESLKEKENEIEKIDKMLKSDLGVNNVFIPLYSKCFDLATKEYTYSVCPYEKASQGHTSLGKFESFGDNgkM 458
Cdd:pfam13015   1 LQMSIDEHEKDIKKIESDITILEENLNSRYGPDDILRAYEGRETKEKIGGYTYKVCFLGSIFQDDISIGNFKKQEGN--K 78

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 256013060  459 MLFENGQQCWGGPKRSLKVLMECGQDNELYDVQEPGKCEYTIKFKTPVLCSEEHLKILRLEGDHS 523
Cdd:pfam13015  79 LYYENGAKCWNGPHRSAIVEVECGDVNELVSVSEPEKCEYLFVVKSPAACSNVYLVKSLAEEESL 143
LDLa smart00192
Low-density lipoprotein receptor domain class A; Cysteine-rich repeat in the low-density ...
 74-110 7.26e-03

Low-density lipoprotein receptor domain class A; Cysteine-rich repeat in the low-density lipoprotein (LDL) receptor that plays a central role in mammalian cholesterol metabolism. The N-terminal type A repeats in LDL receptor bind the lipoproteins. Other homologous domains occur in related receptors, including the very low-density lipoprotein receptor and the LDL receptor-related protein/alpha 2-macroglobulin receptor, and in proteins which are functionally unrelated, such as the C9 component of complement. Mutations in the LDL receptor gene cause familial hypercholesterolemia.


Pssm-ID: 197566  Cd Length: 33  Bit Score: 34.15  E-value: 7.26e-03
                           10        20        30
                   ....*....|....*....|....*....|....*..
gi 256013060    74 CSSNGRFYCQNighkGNYISSSFVNDGVCDCCDGSDE 110
Cdd:smart00192   1 TCPPGEFQCDN----GRCIPSSWVCDGVDDCGDGSDE 33
LDLa cd00112
Low Density Lipoprotein Receptor Class A domain, a cysteine-rich repeat that plays a central ...
 74-110 9.47e-03

Low Density Lipoprotein Receptor Class A domain, a cysteine-rich repeat that plays a central role in mammalian cholesterol metabolism; the receptor protein binds LDL and transports it into cells by endocytosis; 7 successive cysteine-rich repeats of about 40 amino acids are present in the N-terminal of this multidomain membrane protein; other homologous domains occur in related receptors, including the very low-density lipoprotein receptor and the LDL receptor-related protein/alpha 2-macroglobulin receptor, and in proteins which are functionally unrelated, such as the C9 component of complement; the binding of calcium is required for in vitro formation of the native disulfide isomer and is necessary in establishing and maintaining the modular structure


Pssm-ID: 238060  Cd Length: 35  Bit Score: 34.10  E-value: 9.47e-03
                         10        20        30
                 ....*....|....*....|....*....|....*..
gi 256013060  74 CSSNGrFYCQNighkGNYISSSFVNDGVCDCCDGSDE 110
Cdd:cd00112    1 CPPNE-FRCAN----GRCIPSSWVCDGEDDCGDGSDE 32
 
Name Accession Description Interval E-value
PRKCSH-like pfam12999
Glucosidase II beta subunit-like; The sequences found in this family are similar to a region ...
 20-172 2.91e-40

Glucosidase II beta subunit-like; The sequences found in this family are similar to a region found in the beta-subunit of glucosidase II, which is also known as protein kinase C substrate 80K-H (PRKCSH). The enzyme catalyzes the sequential removal of two alpha-1,3-linked glucose residues in the second step of N-linked oligosaccharide processing. The beta subunit is required for the solubility and stability of the heterodimeric enzyme, and is involved in retaining the enzyme within the endoplasmic reticulum.


Pssm-ID: 372423 [Multi-domain]  Cd Length: 176  Bit Score: 143.39  E-value: 2.91e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 256013060   20 SLSPTYGVGPEELEYYK--EGKYFNCL-RSNVQIPFSQVNDDFCDCPDGTDEPGTSACsSNGRFYCQNIGHKGNYISSSF 96
Cdd:pfam12999  15 ALGKLRGVSPDNLHLYQpdENGNWKCLnHSEIKLSFDQVNDDYCDCPDGSDEPGTNAC-SNGKFYCANEGFIPGYIPSFK 93

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 256013060   97 VNDGVCD---CCDGSDEYQlkVKCKNNCKEIGEESRKKQNQVIEAYEIGLKKKKQMEEEGTRvfneKTDEIIRLRKEID 172
Cdd:pfam12999  94 VDDGVCDydiCCDGSDEAL--GKCPNKCGEIARQFEEYLTEHNNSVKNGLKIKEGLLLAAQK----KRDELKKRLKELE 166
PRKCSH_1 pfam13015
Glucosidase II beta subunit-like protein; The sequences found in this family are similar to a ...
 379-523 3.37e-24

Glucosidase II beta subunit-like protein; The sequences found in this family are similar to a region found in the beta-subunit of glucosidase II, which is also known as protein kinase C substrate 80K-H (PRKCSH). The enzyme catalyzes the sequential removal of two alpha-1,3-linked glucose residues in the second step of N-linked oligosaccharide processing. The beta subunit is required for the solubility and stability of the heterodimeric enzyme, and is involved in retaining the enzyme within the endoplasmic reticulum. The beta-subunit confers substrate specificity for di- and monoglucosylated glycans on the glucose-trimming activity of the alpha-subunit.


Pssm-ID: 404038  Cd Length: 154  Bit Score: 98.75  E-value: 3.37e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 256013060  379 LESELSKKRESLKEKENEIEKIDKMLKSDLGVNNVFIPLYSKCFDLATKEYTYSVCPYEKASQGHTSLGKFESFGDNgkM 458
Cdd:pfam13015   1 LQMSIDEHEKDIKKIESDITILEENLNSRYGPDDILRAYEGRETKEKIGGYTYKVCFLGSIFQDDISIGNFKKQEGN--K 78

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 256013060  459 MLFENGQQCWGGPKRSLKVLMECGQDNELYDVQEPGKCEYTIKFKTPVLCSEEHLKILRLEGDHS 523
Cdd:pfam13015  79 LYYENGAKCWNGPHRSAIVEVECGDVNELVSVSEPEKCEYLFVVKSPAACSNVYLVKSLAEEESL 143
PRKCSH pfam07915
Glucosidase II beta subunit-like protein; The sequences found in this family are similar to a ...
 419-467 1.62e-04

Glucosidase II beta subunit-like protein; The sequences found in this family are similar to a region found in the beta-subunit of glucosidase II, which is also known as protein kinase C substrate 80K-H (PRKCSH). The enzyme catalyzes the sequential removal of two alpha-1,3-linked glucose residues in the second step of N-linked oligosaccharide processing. The beta subunit is required for the solubility and stability of the heterodimeric enzyme, and is involved in retaining the enzyme within the endoplasmic reticulum. Mutations in the gene coding for PRKCSH have been found to be involved in the development of autosomal dominant polycystic liver disease (ADPLD), but the precise role the protein has in the pathogenesis of this disease is unknown. This family also includes an ER sensor for misfolded glycoproteins and is therefore likely to be a generic sugar binding domain.


Pssm-ID: 400321  Cd Length: 72  Bit Score: 40.22  E-value: 1.62e-04
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 256013060  419 SKCFDLATKEYTYSVCPYEKASQGH---------TSLGKFESF--------------GDNGKMMLFENGQQC 467
Cdd:pfam07915   1 GKCFYYDEGEWTYEFCFGKHVRQFHkgqekggssFSLGRFSESswaestyddewtkgSNRYISMIYGNGTKC 72
ATP-synt_B pfam00430
ATP synthase B/B' CF(0); Part of the CF(0) (base unit) of the ATP synthase. The base unit is ...
 126-201 4.60e-03

ATP synthase B/B' CF(0); Part of the CF(0) (base unit) of the ATP synthase. The base unit is thought to translocate protons through membrane (inner membrane in mitochondria, thylakoid membrane in plants, cytoplasmic membrane in bacteria). The B subunits are thought to interact with the stalk of the CF(1) subunits. This domain should not be confused with the ab CF(1) proteins (in the head of the ATP synthase) which are found in pfam00006


Pssm-ID: 425677 [Multi-domain]  Cd Length: 132  Bit Score: 37.29  E-value: 4.60e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 256013060  126 EESRKKQNQVIEAYEiglKKKKQMEEEGTRVFNEKTDEIIRLRKEIdpITQEIKELEVLIEQKKSERELENKRLLN 201
Cdd:pfam00430  43 EERRKDAAAALAEAE---QQLKEARAEAQEIIENAKKRAEKLKEEI--VAAAEAEAERIIEQAAAEIEQEKDRALA 113
LDLa smart00192
Low-density lipoprotein receptor domain class A; Cysteine-rich repeat in the low-density ...
 74-110 7.26e-03

Low-density lipoprotein receptor domain class A; Cysteine-rich repeat in the low-density lipoprotein (LDL) receptor that plays a central role in mammalian cholesterol metabolism. The N-terminal type A repeats in LDL receptor bind the lipoproteins. Other homologous domains occur in related receptors, including the very low-density lipoprotein receptor and the LDL receptor-related protein/alpha 2-macroglobulin receptor, and in proteins which are functionally unrelated, such as the C9 component of complement. Mutations in the LDL receptor gene cause familial hypercholesterolemia.


Pssm-ID: 197566  Cd Length: 33  Bit Score: 34.15  E-value: 7.26e-03
                           10        20        30
                   ....*....|....*....|....*....|....*..
gi 256013060    74 CSSNGRFYCQNighkGNYISSSFVNDGVCDCCDGSDE 110
Cdd:smart00192   1 TCPPGEFQCDN----GRCIPSSWVCDGVDDCGDGSDE 33
LDLa cd00112
Low Density Lipoprotein Receptor Class A domain, a cysteine-rich repeat that plays a central ...
 74-110 9.47e-03

Low Density Lipoprotein Receptor Class A domain, a cysteine-rich repeat that plays a central role in mammalian cholesterol metabolism; the receptor protein binds LDL and transports it into cells by endocytosis; 7 successive cysteine-rich repeats of about 40 amino acids are present in the N-terminal of this multidomain membrane protein; other homologous domains occur in related receptors, including the very low-density lipoprotein receptor and the LDL receptor-related protein/alpha 2-macroglobulin receptor, and in proteins which are functionally unrelated, such as the C9 component of complement; the binding of calcium is required for in vitro formation of the native disulfide isomer and is necessary in establishing and maintaining the modular structure


Pssm-ID: 238060  Cd Length: 35  Bit Score: 34.10  E-value: 9.47e-03
                         10        20        30
                 ....*....|....*....|....*....|....*..
gi 256013060  74 CSSNGrFYCQNighkGNYISSSFVNDGVCDCCDGSDE 110
Cdd:cd00112    1 CPPNE-FRCAN----GRCIPSSWVCDGEDDCGDGSDE 32
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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