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Conserved domains on  [gi|256012984|gb|EEU04114|]
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GCN5-related N-acetyltransferase [Dictyostelium discoideum AX4]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 11418877)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  27367672|26818562|15581578|10940244
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 67-155 2.02e-17

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


:

Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 72.77  E-value: 2.02e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 256012984  67 QVQGEQPSLYIMTFCVLAQYRNLGIGRKLLEYIEELCKTEKYEKISLHVQVGSD-AIDFYKKFSFSIESTINNYYRNiqp 145
Cdd:COG0456    6 GLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEaAIALYEKLGFEEVGERPNYYGD--- 82

                         90
                 ....*....|
gi 256012984 146 aDCYVMSKKM 155
Cdd:COG0456   83 -DALVMEKEL 91
 
Name Accession Description Interval E-value
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 67-155 2.02e-17

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 72.77  E-value: 2.02e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 256012984  67 QVQGEQPSLYIMTFCVLAQYRNLGIGRKLLEYIEELCKTEKYEKISLHVQVGSD-AIDFYKKFSFSIESTINNYYRNiqp 145
Cdd:COG0456    6 GLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEaAIALYEKLGFEEVGERPNYYGD--- 82

                         90
                 ....*....|
gi 256012984 146 aDCYVMSKKM 155
Cdd:COG0456   83 -DALVMEKEL 91
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 48-130 7.20e-14

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 64.08  E-value: 7.20e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 256012984   48 LAFFNDIMVGAVCCKIDQsqvqGEQPSLYIMTFCVLAQYRNLGIGRKLLEYIEELCKTEKYEKISLHVQVGSD-AIDFYK 126
Cdd:pfam00583  37 VAEEDGELVGFASLSIID----DEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFLEVAADNLaAIALYE 112


                  ....
gi 256012984  127 KFSF 130
Cdd:pfam00583 113 KLGF 116
rimI TIGR01575
ribosomal-protein-alanine acetyltransferase; Members of this model belong to the GCN5-related ...
 77-151 2.74e-11

ribosomal-protein-alanine acetyltransferase; Members of this model belong to the GCN5-related N-acetyltransferase (GNAT) superfamily. This model covers prokarotes and the archaea. The seed contains a characterized accession for Gram negative E. coli. An untraceable characterized accession (PIR|S66013) for Gram positive B. subtilis scores well (205.0) in the full alignment. Characterized members are lacking in the archaea. Noise cutoff (72.4) was set to exclude M. loti paralog of rimI. Trusted cutoff (80.0) was set at next highest scoring member in the mini-database. [Protein synthesis, Ribosomal proteins: synthesis and modification]


Pssm-ID: 273701 [Multi-domain]  Cd Length: 131  Bit Score: 57.72  E-value: 2.74e-11
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 256012984   77 IMTFCVLAQYRNLGIGRKLLEYIEELCKTEKYEKISLHVQVGSD-AIDFYKKFSFSIESTINNYYRNIQpADCYVM 151
Cdd:TIGR01575  57 ILNIAVKPEYQGQGIGRALLRELIDEAKGRGVNEIFLEVRVSNIaAQALYKKLGFNEIAIRRNYYPDPG-EDAIVM 131
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 48-114 2.27e-10

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 53.82  E-value: 2.27e-10
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 256012984  48 LAFFNDIMVGAVCCKIDQSqvqgEQPSLYIMTFCVLAQYRNLGIGRKLLEYIEELCKTEKYEKISLH 114
Cdd:cd04301    3 VAEDDGEIVGFASLSPDGS----GGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
PRK10140 PRK10140
N-acetyltransferase;
20-153 4.90e-08

N-acetyltransferase;


Pssm-ID: 182263 [Multi-domain]  Cd Length: 162  Bit Score: 49.98  E-value: 4.90e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 256012984  20 VLLNNTTLPVSYEEKFYSKLLSTGFVSKL-AFFNDIMVGAVCCKIDQSQVQGEQPSLYImtfCVLAQYRNLGIGRKLLEY 98
Cdd:PRK10140  26 VYHNTLQVPHPSDHMWQERLADRPGIKQLvACIDGDVVGHLTIDVQQRPRRSHVADFGI---CVDSRWKNRGVASALMRE 102

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 256012984  99 IEELCKTE-KYEKISLHVQVGSD-AIDFYKKFSFSIESTINNY-YRNIQPADCYVMSK 153
Cdd:PRK10140 103 MIEMCDNWlRVDRIELTVFVDNApAIKVYKKYGFEIEGTGKKYaLRNGEYVDAYYMAR 160
 
Name Accession Description Interval E-value
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 67-155 2.02e-17

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 72.77  E-value: 2.02e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 256012984  67 QVQGEQPSLYIMTFCVLAQYRNLGIGRKLLEYIEELCKTEKYEKISLHVQVGSD-AIDFYKKFSFSIESTINNYYRNiqp 145
Cdd:COG0456    6 GLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEaAIALYEKLGFEEVGERPNYYGD--- 82

                         90
                 ....*....|
gi 256012984 146 aDCYVMSKKM 155
Cdd:COG0456   83 -DALVMEKEL 91
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 48-130 7.20e-14

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 64.08  E-value: 7.20e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 256012984   48 LAFFNDIMVGAVCCKIDQsqvqGEQPSLYIMTFCVLAQYRNLGIGRKLLEYIEELCKTEKYEKISLHVQVGSD-AIDFYK 126
Cdd:pfam00583  37 VAEEDGELVGFASLSIID----DEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFLEVAADNLaAIALYE 112


                  ....
gi 256012984  127 KFSF 130
Cdd:pfam00583 113 KLGF 116
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
80-134 8.97e-13

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 61.74  E-value: 8.97e-13
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 256012984  80 FCVLAQYRNLGIGRKLLEYIEELCKTEKYEKISLHVQVgsDAIDFYKKFSFSIES 134
Cdd:COG2153   64 VAVLPEYRGQGLGRALMEAAIEEARERGARRIVLSAQA--HAVGFYEKLGFVPVG 116
rimI TIGR01575
ribosomal-protein-alanine acetyltransferase; Members of this model belong to the GCN5-related ...
 77-151 2.74e-11

ribosomal-protein-alanine acetyltransferase; Members of this model belong to the GCN5-related N-acetyltransferase (GNAT) superfamily. This model covers prokarotes and the archaea. The seed contains a characterized accession for Gram negative E. coli. An untraceable characterized accession (PIR|S66013) for Gram positive B. subtilis scores well (205.0) in the full alignment. Characterized members are lacking in the archaea. Noise cutoff (72.4) was set to exclude M. loti paralog of rimI. Trusted cutoff (80.0) was set at next highest scoring member in the mini-database. [Protein synthesis, Ribosomal proteins: synthesis and modification]


Pssm-ID: 273701 [Multi-domain]  Cd Length: 131  Bit Score: 57.72  E-value: 2.74e-11
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 256012984   77 IMTFCVLAQYRNLGIGRKLLEYIEELCKTEKYEKISLHVQVGSD-AIDFYKKFSFSIESTINNYYRNIQpADCYVM 151
Cdd:TIGR01575  57 ILNIAVKPEYQGQGIGRALLRELIDEAKGRGVNEIFLEVRVSNIaAQALYKKLGFNEIAIRRNYYPDPG-EDAIVM 131
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
11-155 3.01e-11

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 57.79  E-value: 3.01e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 256012984  11 LTDKNLGQLVLLNNTTLPVSYEEKFYSKLLSTGFVSKL--AFFNDIMVGAVCCkiDQSQVQGEQPSLYIMTFCVLAQYRN 88
Cdd:COG3153    4 ATPEDAEAIAALLRAAFGPGREAELVDRLREDPAAGLSlvAEDDGEIVGHVAL--SPVDIDGEGPALLLGPLAVDPEYRG 81

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 256012984  89 LGIGRKLLEYIEELCKTEKYEKISLHvqVGSDAIDFYKKFSFSIESTinnyYRNIQPADCYVMSKKM 155
Cdd:COG3153   82 QGIGRALMRAALEAARERGARAVVLL--GDPSLLPFYERFGFRPAGE----LGLTLGPDEVFLAKEL 142
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 48-132 3.47e-11

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 56.31  E-value: 3.47e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 256012984   48 LAFFNDIMVGAVCCKIDqsqvqGEQPSLYIMTFCVLAQYRNLGIGRKLLEYIEELCKTEKYEKISLHVQVgsDAIDFYKK 127
Cdd:pfam13508   7 VAEDDGKIVGFAALLPL-----DDEGALAELRLAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLELETTN--RAAAFYEK 79


                  ....*
gi 256012984  128 FSFSI 132
Cdd:pfam13508  80 LGFEE 84
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 48-114 2.27e-10

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 53.82  E-value: 2.27e-10
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 256012984  48 LAFFNDIMVGAVCCKIDQSqvqgEQPSLYIMTFCVLAQYRNLGIGRKLLEYIEELCKTEKYEKISLH 114
Cdd:cd04301    3 VAEDDGEIVGFASLSPDGS----GGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 76-155 3.47e-10

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 55.00  E-value: 3.47e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 256012984  76 YIMTFCVLAQYRNLGIGRKLLEYIEELCKTEKYEKISLHvqVGSDAIDFYKKFSFSIESTINNYYRNIQPADCYVMSKKM 155
Cdd:COG1246   54 ELRSLAVHPDYRGRGIGRRLLEALLAEARELGLKRLFLL--TTSAAIHFYEKLGFEEIDKEDLPYAKVWQRDSVVMEKDL 131
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 52-139 3.56e-08

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 49.67  E-value: 3.56e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 256012984  52 NDIMVGAVCCKidqsqvQGEQPSLYIMTFCVLAQYRNLGIGRKLLEYIEELCKTEKYEKISLHVQVGSD-AIDFYKKFSF 130
Cdd:COG0454   42 KGEPIGFAGLR------RLDDKVLELKRLYVLPEYRGKGIGKALLEALLEWARERGCTALELDTLDGNPaAIRFYERLGF 115


                 ....*....
gi 256012984 131 SIESTINNY 139
Cdd:COG0454  116 KEIERYVAY 124
PRK10140 PRK10140
N-acetyltransferase;
20-153 4.90e-08

N-acetyltransferase;


Pssm-ID: 182263 [Multi-domain]  Cd Length: 162  Bit Score: 49.98  E-value: 4.90e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 256012984  20 VLLNNTTLPVSYEEKFYSKLLSTGFVSKL-AFFNDIMVGAVCCKIDQSQVQGEQPSLYImtfCVLAQYRNLGIGRKLLEY 98
Cdd:PRK10140  26 VYHNTLQVPHPSDHMWQERLADRPGIKQLvACIDGDVVGHLTIDVQQRPRRSHVADFGI---CVDSRWKNRGVASALMRE 102

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 256012984  99 IEELCKTE-KYEKISLHVQVGSD-AIDFYKKFSFSIESTINNY-YRNIQPADCYVMSK 153
Cdd:PRK10140 103 MIEMCDNWlRVDRIELTVFVDNApAIKVYKKYGFEIEGTGKKYaLRNGEYVDAYYMAR 160
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 33-130 1.21e-07

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 48.04  E-value: 1.21e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 256012984   33 EKFYSKLLSTGFVSKLAFFNDIMVGAVCckIDQSQvqgeqpslYIMTFCVLAQYRNLGIGRKLLEYIEELCKTEKYEKIS 112
Cdd:pfam13673  20 EALRERIDQGEYFFFVAFEGGQIVGVIA--LRDRG--------HISLLFVDPDYQGQGIGKALLEAVEDYAEKDGIKLSE 89

                          90
                  ....*....|....*...
gi 256012984  113 LHVQVGSDAIDFYKKFSF 130
Cdd:pfam13673  90 LTVNASPYAVPFYEKLGF 107
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
79-153 1.40e-07

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 48.45  E-value: 1.40e-07
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 256012984  79 TFCVLAQYRNLGIGRKLLEYIEELCKTEKYEKISLHVQVGSD-AIDFYKKFSFSIESTINN-YYRNIQPADCYVMSK 153
Cdd:COG1247   85 SIYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLADNEaSIALYEKLGFEEVGTLPEvGFKFGRWLDLVLMQK 161
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
76-130 2.95e-04

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 37.97  E-value: 2.95e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 256012984  76 YIMTFCVLAQYRNLGIGRKLLEYIEELCKTEKYEKISLHVQVGSD-AIDFYKKFSF 130
Cdd:COG3393   17 EISGVYTHPEYRGRGLASALVAALAREALARGARTPFLYVDADNPaARRLYERLGF 72
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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