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Conserved domains on  [gi|22749443|ref|NP_689939|]
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calcium uptake protein 2, mitochondrial [Homo sapiens]

Protein Classification

EFh_MICU2 domain-containing protein( domain architecture ID 11610843)

EFh_MICU2 domain-containing protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
EFh_MICU2 cd16174
EF-hand, calcium binding motif, found in calcium uptake protein 2, mitochondrial (MICU2) and ...
 176-395 1.86e-82

EF-hand, calcium binding motif, found in calcium uptake protein 2, mitochondrial (MICU2) and similar proteins; MICU2, also termed EF-hand domain-containing family member A1 (EFHA1), is a mitochondrial-localized paralog of MICU1. MICU2 and its paralog, MICU1, are physically associated within the mitochondrial calcium uniporter (MCU) complex and are co-expressed across all tissues. They may operate together with MCU to regulate the channel. At present, the precise molecular function of MICU2 remains unclear. It may play possible roles in Ca2+ sensing and regulation of MCU, calcium buffering with a secondary impact on transport or assembly and stabilization of MCU. MICU2 contains an N-terminal mitochondrial targeting sequence (MTS) as well as two evolutionarily conserved canonical Ca2+-binding EF-hands separated by a long stretch of residues predicted to form alpha-helices.


:

Pssm-ID: 320082 [Multi-domain]  Cd Length: 154  Bit Score: 250.17  E-value: 1.86e-82
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22749443 176 GFHVAFKMLDTDGNEMIEKREFFKLQKIISKQDDLMTVKTNETgYQEAIVKEPEINTTLQMRFFGKRGQRKLHYKEFRRF 255
Cdd:cd16174   1 GFHIAFKMLDTDGNEQVEKREFFKLQKIIGKKDDLMTQGGTET-YQEASDNSDEVNTTLQVHFFGKDGNEKLQYKEFCRF 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22749443 256 MENLQTEIqemeflqfskglsfmrkedfaewllfftntenkdiywknvreklsagesisldefksfchftthlEDFAIAM 335
Cdd:cd16174  80 MENLQTEV-----------------------------------------------------------------EDFAIAM 94

                       170       180       190       200       210       220
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 22749443 336 QMFSLAHRPVRLAEFKRAVKVATGQELSNNILDTVFKIFDLDGDECLSHEEFLGVLKNRM 395
Cdd:cd16174  95 KMFSEANRPIKLAEFKRAVKVATGQELSDNVLDTVFKIFDLDGDDCLSHGEFLGVLKNRV 154
 
Name Accession Description Interval E-value
EFh_MICU2 cd16174
EF-hand, calcium binding motif, found in calcium uptake protein 2, mitochondrial (MICU2) and ...
 176-395 1.86e-82

EF-hand, calcium binding motif, found in calcium uptake protein 2, mitochondrial (MICU2) and similar proteins; MICU2, also termed EF-hand domain-containing family member A1 (EFHA1), is a mitochondrial-localized paralog of MICU1. MICU2 and its paralog, MICU1, are physically associated within the mitochondrial calcium uniporter (MCU) complex and are co-expressed across all tissues. They may operate together with MCU to regulate the channel. At present, the precise molecular function of MICU2 remains unclear. It may play possible roles in Ca2+ sensing and regulation of MCU, calcium buffering with a secondary impact on transport or assembly and stabilization of MCU. MICU2 contains an N-terminal mitochondrial targeting sequence (MTS) as well as two evolutionarily conserved canonical Ca2+-binding EF-hands separated by a long stretch of residues predicted to form alpha-helices.


Pssm-ID: 320082 [Multi-domain]  Cd Length: 154  Bit Score: 250.17  E-value: 1.86e-82
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22749443 176 GFHVAFKMLDTDGNEMIEKREFFKLQKIISKQDDLMTVKTNETgYQEAIVKEPEINTTLQMRFFGKRGQRKLHYKEFRRF 255
Cdd:cd16174   1 GFHIAFKMLDTDGNEQVEKREFFKLQKIIGKKDDLMTQGGTET-YQEASDNSDEVNTTLQVHFFGKDGNEKLQYKEFCRF 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22749443 256 MENLQTEIqemeflqfskglsfmrkedfaewllfftntenkdiywknvreklsagesisldefksfchftthlEDFAIAM 335
Cdd:cd16174  80 MENLQTEV-----------------------------------------------------------------EDFAIAM 94

                       170       180       190       200       210       220
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 22749443 336 QMFSLAHRPVRLAEFKRAVKVATGQELSNNILDTVFKIFDLDGDECLSHEEFLGVLKNRM 395
Cdd:cd16174  95 KMFSEANRPIKLAEFKRAVKVATGQELSDNVLDTVFKIFDLDGDDCLSHGEFLGVLKNRV 154
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
311-388 1.81e-04

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 41.32  E-value: 1.81e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22749443 311 ESISLDEFKSFCHFTTHLEDFAIAMQMFSLAHRP----VRLAEFKRAVKVAtgqELSNNILDTVFKIFDLDGDECLSHEE 386
Cdd:COG5126  48 GRISREEFVAGMESLFEATVEPFARAAFDLLDTDgdgkISADEFRRLLTAL---GVSEEEADELFARLDTDGDGKISFEE 124


                ..
gi 22749443 387 FL 388
Cdd:COG5126 125 FV 126
EF-hand_8 pfam13833
EF-hand domain pair;
348-394 4.69e-04

EF-hand domain pair;


Pssm-ID: 404678 [Multi-domain]  Cd Length: 54  Bit Score: 38.06  E-value: 4.69e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*..
gi 22749443   348 AEFKRAVKVATGQELSNNILDTVFKIFDLDGDECLSHEEFLGVLKNR 394
Cdd:pfam13833   8 EELKRALALLGLKDLSEDEVDILFREFDTDGDGYISFDEFCVLLERR 54
 
Name Accession Description Interval E-value
EFh_MICU2 cd16174
EF-hand, calcium binding motif, found in calcium uptake protein 2, mitochondrial (MICU2) and ...
 176-395 1.86e-82

EF-hand, calcium binding motif, found in calcium uptake protein 2, mitochondrial (MICU2) and similar proteins; MICU2, also termed EF-hand domain-containing family member A1 (EFHA1), is a mitochondrial-localized paralog of MICU1. MICU2 and its paralog, MICU1, are physically associated within the mitochondrial calcium uniporter (MCU) complex and are co-expressed across all tissues. They may operate together with MCU to regulate the channel. At present, the precise molecular function of MICU2 remains unclear. It may play possible roles in Ca2+ sensing and regulation of MCU, calcium buffering with a secondary impact on transport or assembly and stabilization of MCU. MICU2 contains an N-terminal mitochondrial targeting sequence (MTS) as well as two evolutionarily conserved canonical Ca2+-binding EF-hands separated by a long stretch of residues predicted to form alpha-helices.


Pssm-ID: 320082 [Multi-domain]  Cd Length: 154  Bit Score: 250.17  E-value: 1.86e-82
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22749443 176 GFHVAFKMLDTDGNEMIEKREFFKLQKIISKQDDLMTVKTNETgYQEAIVKEPEINTTLQMRFFGKRGQRKLHYKEFRRF 255
Cdd:cd16174   1 GFHIAFKMLDTDGNEQVEKREFFKLQKIIGKKDDLMTQGGTET-YQEASDNSDEVNTTLQVHFFGKDGNEKLQYKEFCRF 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22749443 256 MENLQTEIqemeflqfskglsfmrkedfaewllfftntenkdiywknvreklsagesisldefksfchftthlEDFAIAM 335
Cdd:cd16174  80 MENLQTEV-----------------------------------------------------------------EDFAIAM 94

                       170       180       190       200       210       220
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 22749443 336 QMFSLAHRPVRLAEFKRAVKVATGQELSNNILDTVFKIFDLDGDECLSHEEFLGVLKNRM 395
Cdd:cd16174  95 KMFSEANRPIKLAEFKRAVKVATGQELSDNVLDTVFKIFDLDGDDCLSHGEFLGVLKNRV 154
EFh_MICU cd15900
EF-hand, calcium binding motif, found in mitochondrial calcium uptake proteins MICU1, MICU2, ...
 176-395 4.89e-56

EF-hand, calcium binding motif, found in mitochondrial calcium uptake proteins MICU1, MICU2, MICU3, and similar proteins; This family includes mitochondrial calcium uptake protein MICU1 and its two additional paralogs, MICU2 and MICU3. MICU1 localizes to the inner mitochondrial membrane (IMM). It functions as a gatekeeper of the mitochondrial calcium uniporter (MCU) and regulates MCU-mediated mitochondrial Ca2+ uptake, which is essential for maintaining mitochondrial homoeostasis. MICU1 and MICU2 are physically associated within the uniporter complex and are co-expressed across all tissues. They may play non-redundant roles in the regulation of the mitochondrial calcium uniporter. At present, the precise molecular function of MICU2 and MICU3 remain unclear. MICU2 may play possible roles in Ca2+ sensing and regulation of MCU, calcium buffering with a secondary impact on transport or assembly and stabilization of MCU. MICU3 likely has a role in mitochondrial calcium handling. All members in this family contains an N-terminal mitochondrial targeting sequence (MTS) as well as two evolutionarily conserved canonical Ca2+-binding EF-hands separated by a long stretch of residues predicted to form alpha-helices.


Pssm-ID: 320080 [Multi-domain]  Cd Length: 152  Bit Score: 182.04  E-value: 4.89e-56
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22749443 176 GFHVAFKMLDTDGNEMIEKREFFKLQKIISKQDDLMTVKTNETGyqeAIVKEPEINTTLQMRFFGKRGQRKLHYKEFRRF 255
Cdd:cd15900   1 HFEIAFKMFDLDGDGELDKEEFNKVQSIIRSQTSVGQRHRDHTN---GESTKLGMNSTLARYFFGKDGKQKLSIEKFLEF 77

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22749443 256 MENLQTEIqemeflqfskglsfmrkedfaewllfftntenkdiywknvreklsagesisldefksfchftthlEDFAIAM 335
Cdd:cd15900  78 QENLQEEI-----------------------------------------------------------------DDVDTAL 92

                       170       180       190       200       210       220
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 22749443 336 QMFSLAHRPVRLAEFKRAVKVATGQELSNNILDTVFKIFDLDGDECLSHEEFLGVLKNRM 395
Cdd:cd15900  93 TFYHLAGASIDRKTFKRAAKVVAGVELSDHVVDVVFTIFDEDGDGILSHKEFISVMKDRL 152
EFh_MICU3 cd16175
EF-hand, calcium binding motif, found in calcium uptake protein 3, mitochondrial (MICU3) and ...
 176-395 1.01e-35

EF-hand, calcium binding motif, found in calcium uptake protein 3, mitochondrial (MICU3) and similar proteins; MICU3, also termed EF-hand domain-containing family member A2 (EFHA2), is a paralog of MICU1 and notably found in the central nervous system (CNS) and skeletal muscle. At present, the precise molecular function of MICU3 remains unclear. It likely has a role in mitochondrial calcium handling. MICU3 contains an N-terminal mitochondrial targeting sequence (MTS) as well as two evolutionarily conserved canonical Ca2+-binding EF-hands separated by a long stretch of residues predicted to form alpha-helices.


Pssm-ID: 320083 [Multi-domain]  Cd Length: 128  Bit Score: 128.02  E-value: 1.01e-35
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22749443 176 GFHVAFKMLDTDGNEMIEKREFFKLQKIISkqddlmtvktnetgyqeaivkepeintTLQMRFFGKRGQRKLHYKEFRRF 255
Cdd:cd16175   1 GFRIAFNMFDTDGNEMVDKKEFLVLQEIFR---------------------------TLLVHFFGKKGKAELNFEDFYRF 53

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22749443 256 MENLQTEIqemeflqfskglsfmrkedfaewllfftntenkdiywknvreklsagesisldefksfchftthlEDFAIAM 335
Cdd:cd16175  54 MDNLQTEV-----------------------------------------------------------------EDFTIAM 68

                       170       180       190       200       210       220
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 22749443 336 QMFSLAHRPVRLAEFKRAVKVATGQELSNNILDTVFKIFDLDGDECLSHEEFLGVLKNRM 395
Cdd:cd16175  69 RMYTFADRSISQDEFARAVKVCTGLKLSPHLVNTVFKIFDVDGDGQLSYKEFIGIMKDRL 128
EFh_MICU1 cd16173
EF-hand, calcium binding motif, found in calcium uptake protein 1, mitochondrial (MICU1) and ...
 177-395 1.01e-05

EF-hand, calcium binding motif, found in calcium uptake protein 1, mitochondrial (MICU1) and similar proteins; MICU1, also termed atopy-related autoantigen CALC (ara CALC), or calcium-binding atopy-related autoantigen 1 (CBARA1), or Hom s 4, or EFHA3, localizes to the inner mitochondrial membrane (IMM). It functions as a gatekeeper of the mitochondrial calcium uniporter (MCU) and regulates MCU-mediated mitochondrial Ca2+ uptake, which is essential for maintaining mitochondrial homoeostasis. MICU1 and its paralog, MICU2, are physically associated within the uniporter complex and are co-expressed across all tissues. They may operate together with MCU to regulate the channel. The mutations in MICU1 are associated with neuromuscular abnormalities in children. MICU1 contains an N-terminal mitochondrial targeting sequence (MTS) as well as two evolutionarily conserved canonical Ca2+-binding EF-hands separated by a long stretch of residues predicted to form alpha-helices.


Pssm-ID: 320081 [Multi-domain]  Cd Length: 153  Bit Score: 45.40  E-value: 1.01e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22749443 177 FHVAFKMLDTDGNEMIEKREFFKLQKIISKQDDL-MTVKTNETGyqeAIVKEPEINTTLQMRFFGKRGQRKLHYKEFRRF 255
Cdd:cd16173   2 FEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMgMRHRDRSTT---GNTLKTGFSSALTTYFFGADLKGKLTIKNFLEF 78

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22749443 256 MENLQTEIQEMEflqfskglsfmrkedfaewllfftntenkdiywknvreklsagesisldefksfchftthledfaIAM 335
Cdd:cd16173  79 QRKLQHDVNDVD-----------------------------------------------------------------TAL 93

                       170       180       190       200       210       220
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 22749443 336 QMFSLAHRPVRLAEFKRAVKVATGQELSNNILDTVFKIFDLDGDECLSHEEFLGVLKNRM 395
Cdd:cd16173  94 SFYHMAGASLDKVTMQQVARTVAKVELSDHVCDVVFALFDCDGNGELSNKEFVAIMKQRL 153
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
311-388 1.81e-04

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 41.32  E-value: 1.81e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 22749443 311 ESISLDEFKSFCHFTTHLEDFAIAMQMFSLAHRP----VRLAEFKRAVKVAtgqELSNNILDTVFKIFDLDGDECLSHEE 386
Cdd:COG5126  48 GRISREEFVAGMESLFEATVEPFARAAFDLLDTDgdgkISADEFRRLLTAL---GVSEEEADELFARLDTDGDGKISFEE 124


                ..
gi 22749443 387 FL 388
Cdd:COG5126 125 FV 126
EF-hand_8 pfam13833
EF-hand domain pair;
348-394 4.69e-04

EF-hand domain pair;


Pssm-ID: 404678 [Multi-domain]  Cd Length: 54  Bit Score: 38.06  E-value: 4.69e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*..
gi 22749443   348 AEFKRAVKVATGQELSNNILDTVFKIFDLDGDECLSHEEFLGVLKNR 394
Cdd:pfam13833   8 EELKRALALLGLKDLSEDEVDILFREFDTDGDGYISFDEFCVLLERR 54
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
151-202 5.76e-03

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 37.08  E-value: 5.76e-03
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|..
gi 22749443 151 DLGDKGLISYTEYLFLLTILTKPHSGFHVAFKMLDTDGNEMIEKREFFKLQK 202
Cdd:COG5126  79 DTDGDGKISADEFRRLLTALGVSEEEADELFARLDTDGDGKISFEEFVAAVR 130
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 349-388 6.19e-03

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 35.22  E-value: 6.19e-03
                        10        20        30        40
                ....*....|....*....|....*....|....*....|
gi 22749443 349 EFKRAVKvATGQELSNNILDTVFKIFDLDGDECLSHEEFL 388
Cdd:cd00051  21 ELKAALK-SLGEGLSEEEIDEMIREVDKDGDGKIDFEEFL 59
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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