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Conserved domains on  [gi|2127777007|gb|UEC03221|]
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hypothetical protein LK462_06870 [Burkholderia vietnamiensis]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
DHFR super family cl17279
Dihydrofolate reductase (DHFR). Reduces 7,8-dihydrofolate to 5,6,7,8-tetrahydrofolate with ...
 1-39 1.73e-03

Dihydrofolate reductase (DHFR). Reduces 7,8-dihydrofolate to 5,6,7,8-tetrahydrofolate with NADPH as a cofactor. This is an essential step in the biosynthesis of deoxythymidine phosphate since 5,6,7,8-tetrahydrofolate is required to regenerate 5,10-methylenetetrahydrofolate which is then utilized by thymidylate synthase. Inhibition of DHFR interrupts thymidilate synthesis and DNA replication, inhibitors of DHFR (such as Methotrexate) are used in cancer chemotherapy. 5,6,7,8-tetrahydrofolate also is involved in glycine, serine, and threonine metabolism and aminoacyl-tRNA biosynthesis.


The actual alignment was detected with superfamily member PRK10786:

Pssm-ID: 473077 [Multi-domain]  Cd Length: 367  Bit Score: 33.59  E-value: 1.73e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|.
gi 2127777007   1 MERA--LAQRSLYTTDPNQRMGYVLVKNGVVRLPSFPQWAG 39
Cdd:PRK10786    7 MARAlkLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQRAG 47
 
Name Accession Description Interval E-value
ribD PRK10786
bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6- ...
 1-39 1.73e-03

bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD;


Pssm-ID: 182729 [Multi-domain]  Cd Length: 367  Bit Score: 33.59  E-value: 1.73e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|.
gi 2127777007   1 MERA--LAQRSLYTTDPNQRMGYVLVKNGVVRLPSFPQWAG 39
Cdd:PRK10786    7 MARAlkLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQRAG 47
 
Name Accession Description Interval E-value
ribD PRK10786
bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6- ...
 1-39 1.73e-03

bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD;


Pssm-ID: 182729 [Multi-domain]  Cd Length: 367  Bit Score: 33.59  E-value: 1.73e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|.
gi 2127777007   1 MERA--LAQRSLYTTDPNQRMGYVLVKNGVVRLPSFPQWAG 39
Cdd:PRK10786    7 MARAlkLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQRAG 47
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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