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Conserved domains on  [gi|206597190|dbj|BAG71502|]
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spectinomycin adenyltransferase (plasmid) [Plasmid vector pTANS191]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK13746 super family cl32927
aminoglycoside resistance protein; Provisional
 10-232 2.84e-48

aminoglycoside resistance protein; Provisional


The actual alignment was detected with superfamily member PRK13746:

Pssm-ID: 184296 [Multi-domain]  Cd Length: 262  Bit Score: 160.19  E-value: 2.84e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 206597190  10 EQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPNSDLDFLVVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTI 89
Cdd:PRK13746  11 TQLSEACAVIERHLEPTLLAIHLYGSAVDGGLKPHSDIDLLVTVAVPLDETTRRALMNDLLEVSAPPGESEILRALEVTV 90

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 206597190  90 IIQQEMVPWNHPPKQEFIYGEWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNyDLEELLPDIPFSDVRRAIMDS 169
Cdd:PRK13746  91 VVHDDVVPWRYPAKRELQFGEWLRNDILAGIFEPAMIDHDLAILLTKAREHSVALVGP-AAEELFDPVPEQDLFEALRDT 169

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 206597190 170 ----SEEliDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERILLAVRSYLGE 232
Cdd:PRK13746 170 lalwNSP--PDWAGDERNVVLTLARIWYSASTGKIAPKDVAADWALERLPAEYQPVLLEARQAYLGQ 234
 
Name Accession Description Interval E-value
PRK13746 PRK13746
aminoglycoside resistance protein; Provisional
 10-232 2.84e-48

aminoglycoside resistance protein; Provisional


Pssm-ID: 184296 [Multi-domain]  Cd Length: 262  Bit Score: 160.19  E-value: 2.84e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 206597190  10 EQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPNSDLDFLVVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTI 89
Cdd:PRK13746  11 TQLSEACAVIERHLEPTLLAIHLYGSAVDGGLKPHSDIDLLVTVAVPLDETTRRALMNDLLEVSAPPGESEILRALEVTV 90

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 206597190  90 IIQQEMVPWNHPPKQEFIYGEWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNyDLEELLPDIPFSDVRRAIMDS 169
Cdd:PRK13746  91 VVHDDVVPWRYPAKRELQFGEWLRNDILAGIFEPAMIDHDLAILLTKAREHSVALVGP-AAEELFDPVPEQDLFEALRDT 169

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 206597190 170 ----SEEliDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERILLAVRSYLGE 232
Cdd:PRK13746 170 lalwNSP--PDWAGDERNVVLTLARIWYSASTGKIAPKDVAADWALERLPAEYQPVLLEARQAYLGQ 234
DUF4111 pfam13427
Domain of unknown function (DUF4111); Although the exact function of this domain is not known ...
 152-248 3.56e-20

Domain of unknown function (DUF4111); Although the exact function of this domain is not known it frequently appears downstream of the family Nucleotidyltransferase, pfam01909. It is also found in species associated with methicillin-resistant bacteria.


Pssm-ID: 433197  Cd Length: 102  Bit Score: 82.25  E-value: 3.56e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 206597190  152 ELLPDIPFSDVRRAIMDSSEELIDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERILLAVRSYLG 231
Cdd:pfam13427   1 EVFPPVPRADYRDAILADVPELLEEIEEDPRYVVLTLARVWATLETGEILSKDEAAEWALPRLPKEYRPLLAQARDAYLG 80

                          90       100
                  ....*....|....*....|
gi 206597190  232 ---ENIEWTNENVNLTINYL 248
Cdd:pfam13427  81 gaeEDWDGDREELEEFADYM 100
MJ0604 COG1708
Predicted nucleotidyltransferase, MJ0604 family [General function prediction only];
1-98 2.54e-05

Predicted nucleotidyltransferase, MJ0604 family [General function prediction only];


Pssm-ID: 441314  Cd Length: 95  Bit Score: 41.94  E-value: 2.54e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 206597190   1 MRRIYLntyEQINKVKKILRKHLKNNLIgtYMFGSGVESGLKPNSDLDFLVVVSEPLTDQSKeilIQKIRPISKKIGdks 80
Cdd:COG1708    1 MSTLLR---ELLEEIVEALRRGPEVAAV--YLFGSYARGDARPDSDIDLLVVVDDPPLPDER---LELLADLLRELG--- 69

                         90
                 ....*....|....*...
gi 206597190  81 nlRYIELTIIIQQEMVPW 98
Cdd:COG1708   70 --LPVDLVVLTPAELELR 85
NT_KNTase_like cd05403
Nucleotidyltransferase (NT) domain of Staphylococcus aureus kanamycin nucleotidyltransferase, ...
 10-70 4.81e-05

Nucleotidyltransferase (NT) domain of Staphylococcus aureus kanamycin nucleotidyltransferase, and similar proteins; S. aureus KNTase is a plasmid encoded enzyme which confers resistance to a wide range of aminoglycoside antibiotics which have a 4'- or 4''-hydroxyl group in the equatorial position, such as kanamycin A. This enzyme transfers a nucleoside monophosphate group from a nucleotide (ATP,GTP, or UTP) to the 4'-hydroxyl group of kanamycin A. This enzyme is a homodimer, having two NT active sites. The nucleotide and antibiotic binding sites of each active site include residues from each monomer. Included in this subgroup is Escherichia coli AadA5 which confers resistance to the antibiotic spectinomycin and is a putative aminoglycoside-3'-adenylyltransferase. It is part of the aadA5 cassette of a class 1 integron. This subgroup also includes Haemophilus influenzae HI0073 which forms a 2:2 heterotetramer with an unrelated protein HI0074. Structurally HI0074 is related to the substrate-binding domain of S. aureus KNTase. The genes encoding HI0073 and HI0074 form an operon. Little is known about the substrate specificity or function of two-component NTs. The characterized members of this subgroup may not be representive of the function of this subgroup. This subgroup belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, co-ordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are conserved in this subgroup.


Pssm-ID: 143393  Cd Length: 93  Bit Score: 41.25  E-value: 4.81e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 206597190  10 EQINKVKKILRKHLKNnLIGTYMFGSGVESGLKPNSDLDFLVVVSEPLTDQSKEILIQKIR 70
Cdd:cd05403    2 EILEEILEILRELLGG-VEKVYLFGSYARGDARPDSDIDLLVIFDDPLDPLELARLLEELE 61
 
Name Accession Description Interval E-value
PRK13746 PRK13746
aminoglycoside resistance protein; Provisional
 10-232 2.84e-48

aminoglycoside resistance protein; Provisional


Pssm-ID: 184296 [Multi-domain]  Cd Length: 262  Bit Score: 160.19  E-value: 2.84e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 206597190  10 EQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPNSDLDFLVVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTI 89
Cdd:PRK13746  11 TQLSEACAVIERHLEPTLLAIHLYGSAVDGGLKPHSDIDLLVTVAVPLDETTRRALMNDLLEVSAPPGESEILRALEVTV 90

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 206597190  90 IIQQEMVPWNHPPKQEFIYGEWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNyDLEELLPDIPFSDVRRAIMDS 169
Cdd:PRK13746  91 VVHDDVVPWRYPAKRELQFGEWLRNDILAGIFEPAMIDHDLAILLTKAREHSVALVGP-AAEELFDPVPEQDLFEALRDT 169

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 206597190 170 ----SEEliDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERILLAVRSYLGE 232
Cdd:PRK13746 170 lalwNSP--PDWAGDERNVVLTLARIWYSASTGKIAPKDVAADWALERLPAEYQPVLLEARQAYLGQ 234
DUF4111 pfam13427
Domain of unknown function (DUF4111); Although the exact function of this domain is not known ...
 152-248 3.56e-20

Domain of unknown function (DUF4111); Although the exact function of this domain is not known it frequently appears downstream of the family Nucleotidyltransferase, pfam01909. It is also found in species associated with methicillin-resistant bacteria.


Pssm-ID: 433197  Cd Length: 102  Bit Score: 82.25  E-value: 3.56e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 206597190  152 ELLPDIPFSDVRRAIMDSSEELIDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERILLAVRSYLG 231
Cdd:pfam13427   1 EVFPPVPRADYRDAILADVPELLEEIEEDPRYVVLTLARVWATLETGEILSKDEAAEWALPRLPKEYRPLLAQARDAYLG 80

                          90       100
                  ....*....|....*....|
gi 206597190  232 ---ENIEWTNENVNLTINYL 248
Cdd:pfam13427  81 gaeEDWDGDREELEEFADYM 100
MJ0604 COG1708
Predicted nucleotidyltransferase, MJ0604 family [General function prediction only];
1-98 2.54e-05

Predicted nucleotidyltransferase, MJ0604 family [General function prediction only];


Pssm-ID: 441314  Cd Length: 95  Bit Score: 41.94  E-value: 2.54e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 206597190   1 MRRIYLntyEQINKVKKILRKHLKNNLIgtYMFGSGVESGLKPNSDLDFLVVVSEPLTDQSKeilIQKIRPISKKIGdks 80
Cdd:COG1708    1 MSTLLR---ELLEEIVEALRRGPEVAAV--YLFGSYARGDARPDSDIDLLVVVDDPPLPDER---LELLADLLRELG--- 69

                         90
                 ....*....|....*...
gi 206597190  81 nlRYIELTIIIQQEMVPW 98
Cdd:COG1708   70 --LPVDLVVLTPAELELR 85
NT_KNTase_like cd05403
Nucleotidyltransferase (NT) domain of Staphylococcus aureus kanamycin nucleotidyltransferase, ...
 10-70 4.81e-05

Nucleotidyltransferase (NT) domain of Staphylococcus aureus kanamycin nucleotidyltransferase, and similar proteins; S. aureus KNTase is a plasmid encoded enzyme which confers resistance to a wide range of aminoglycoside antibiotics which have a 4'- or 4''-hydroxyl group in the equatorial position, such as kanamycin A. This enzyme transfers a nucleoside monophosphate group from a nucleotide (ATP,GTP, or UTP) to the 4'-hydroxyl group of kanamycin A. This enzyme is a homodimer, having two NT active sites. The nucleotide and antibiotic binding sites of each active site include residues from each monomer. Included in this subgroup is Escherichia coli AadA5 which confers resistance to the antibiotic spectinomycin and is a putative aminoglycoside-3'-adenylyltransferase. It is part of the aadA5 cassette of a class 1 integron. This subgroup also includes Haemophilus influenzae HI0073 which forms a 2:2 heterotetramer with an unrelated protein HI0074. Structurally HI0074 is related to the substrate-binding domain of S. aureus KNTase. The genes encoding HI0073 and HI0074 form an operon. Little is known about the substrate specificity or function of two-component NTs. The characterized members of this subgroup may not be representive of the function of this subgroup. This subgroup belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, co-ordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are conserved in this subgroup.


Pssm-ID: 143393  Cd Length: 93  Bit Score: 41.25  E-value: 4.81e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 206597190  10 EQINKVKKILRKHLKNnLIGTYMFGSGVESGLKPNSDLDFLVVVSEPLTDQSKEILIQKIR 70
Cdd:cd05403    2 EILEEILEILRELLGG-VEKVYLFGSYARGDARPDSDIDLLVIFDDPLDPLELARLLEELE 61
NTP_transf_2 pfam01909
Nucleotidyltransferase domain; Members of this family belong to a large family of ...
 12-86 2.08e-03

Nucleotidyltransferase domain; Members of this family belong to a large family of nucleotidyltransferases. This family includes kanamycin nucleotidyltransferase (KNTase) which is a plasmid-coded enzyme responsible for some types of bacterial resistance to aminoglycosides. KNTase in-activates antibiotics by catalysing the addition of a nucleotidyl group onto the drug.


Pssm-ID: 396474  Cd Length: 91  Bit Score: 36.62  E-value: 2.08e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 206597190   12 INKVKKILRKHLKNNLIgtYMFGSGVESGLKPNSDLDFLVVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIE 86
Cdd:pfam01909   1 LRKLREILKELFPVAEV--VLFGSYARGTALPGSDIDLLVVFPEPVEEERLLKLAKIIKELEELLGLEVDLVTRE 73
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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