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Conserved domains on  [gi|2064674935|ref|NP_001382374|]
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myosin light chain 5 isoform 2 [Homo sapiens]

Protein Classification

EF-hand domain-containing protein( domain architecture ID 1000080)

EF-hand (EFh) domain-containing protein may be involved in binding intracellular calcium and in calcium signal transduction

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PTZ00184 super family cl33172
calmodulin; Provisional
 2-117 3.71e-15

calmodulin; Provisional


The actual alignment was detected with superfamily member PTZ00184:

Pssm-ID: 185504 [Multi-domain]  Cd Length: 149  Bit Score: 67.09  E-value: 3.71e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2064674935   2 DQNRDGFIDKEDLKDTYASLGKtNVKDDELDAMLKE----ASGPINFTMFLNLFGEKLSGTDAEETILNAFKMLDPDGKG 77
Cdd:PTZ00184   21 DKDGDGTITTKELGTVMRSLGQ-NPTEAELQDMINEvdadGNGTIDFPEFLTLMARKMKDTDSEEEIKEAFKVFDRDGNG 99

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 2064674935  78 KINKEYIKRLLMSQADKMTAEEVDQMFQFASIDVAGNLDY 117
Cdd:PTZ00184  100 FISAAELRHVMTNLGEKLTDEEVDEMIREADVDGDGQINY 139
 
Name Accession Description Interval E-value
PTZ00184 PTZ00184
calmodulin; Provisional
 2-117 3.71e-15

calmodulin; Provisional


Pssm-ID: 185504 [Multi-domain]  Cd Length: 149  Bit Score: 67.09  E-value: 3.71e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2064674935   2 DQNRDGFIDKEDLKDTYASLGKtNVKDDELDAMLKE----ASGPINFTMFLNLFGEKLSGTDAEETILNAFKMLDPDGKG 77
Cdd:PTZ00184   21 DKDGDGTITTKELGTVMRSLGQ-NPTEAELQDMINEvdadGNGTIDFPEFLTLMARKMKDTDSEEEIKEAFKVFDRDGNG 99

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 2064674935  78 KINKEYIKRLLMSQADKMTAEEVDQMFQFASIDVAGNLDY 117
Cdd:PTZ00184  100 FISAAELRHVMTNLGEKLTDEEVDEMIREADVDGDGQINY 139
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
1-114 5.34e-07

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 45.55  E-value: 5.34e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2064674935   1 MDQNRDGFIDKEDLKDTYASLGKTNVKDDELDAmlkeaSGPINFTMFLNlFGEKLSGTDAEETILNAFKMLDPDGKGKIN 80
Cdd:COG5126    14 LDADGDGVLERDDFEALFRRLWATLFSEADTDG-----DGRISREEFVA-GMESLFEATVEPFARAAFDLLDTDGDGKIS 87

                          90       100       110
                  ....*....|....*....|....*....|....
gi 2064674935  81 KEYIKRLLmsQADKMTAEEVDQMfqFASIDVAGN 114
Cdd:COG5126    88 ADEFRRLL--TALGVSEEEADEL--FARLDTDGD 117
EFh_calglandulin_like cd16252
EF-hand, calcium binding motif, found in uncharacterized calglandulin-like proteins; The ...
 40-125 6.90e-07

EF-hand, calcium binding motif, found in uncharacterized calglandulin-like proteins; The family corresponds to a group of uncharacterized calglandulin-like proteins. Although their biological function remain unclear, they show high sequence similarity with human calglandulin-like protein GAGLP, which is an ortholog of calglandulin from the venom glands of Bothrops insularis snake. Both GAGLP and calglandulin are putative Ca2+-binding proteins with four EF-hand motifs. However, members in this family contain only three EF-hand motifs. In this point, they may belong to the parvalbumin-like EF-hand family, which is characterized by the presence of three consecutive EF-hand motifs (helix-loop-helix).


Pssm-ID: 319995 [Multi-domain]  Cd Length: 106  Bit Score: 44.83  E-value: 6.90e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2064674935  40 GPINFTMFLNLFGEKLSGTDAEETILNAFKMLDPDGKGKINKEYIKRLLMSQADKM-----TAEEVDQMFQFASIDVAGN 114
Cdd:cd16252    15 GSFNYSKFFEYMQKFQTSEQQEEAIRKAFQMLDKDKSGFIEWNEIKYILSTVPSSMpvaplSDEEAEAMIQAADTDGDGR 94

                          90
                  ....*....|.
gi 2064674935 115 LDYKALSYVIT 125
Cdd:cd16252    95 IDFQEFSDMVK 105
EF-hand_8 pfam13833
EF-hand domain pair;
6-51 3.70e-04

EF-hand domain pair;


Pssm-ID: 404678 [Multi-domain]  Cd Length: 54  Bit Score: 36.14  E-value: 3.70e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 2064674935   6 DGFIDKEDLKDTYASLGKTNVKDDELDAMLKEA----SGPINFTMFLNLF 51
Cdd:pfam13833   2 KGVITREELKRALALLGLKDLSEDEVDILFREFdtdgDGYISFDEFCVLL 51
 
Name Accession Description Interval E-value
PTZ00184 PTZ00184
calmodulin; Provisional
 2-117 3.71e-15

calmodulin; Provisional


Pssm-ID: 185504 [Multi-domain]  Cd Length: 149  Bit Score: 67.09  E-value: 3.71e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2064674935   2 DQNRDGFIDKEDLKDTYASLGKtNVKDDELDAMLKE----ASGPINFTMFLNLFGEKLSGTDAEETILNAFKMLDPDGKG 77
Cdd:PTZ00184   21 DKDGDGTITTKELGTVMRSLGQ-NPTEAELQDMINEvdadGNGTIDFPEFLTLMARKMKDTDSEEEIKEAFKVFDRDGNG 99

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 2064674935  78 KINKEYIKRLLMSQADKMTAEEVDQMFQFASIDVAGNLDY 117
Cdd:PTZ00184  100 FISAAELRHVMTNLGEKLTDEEVDEMIREADVDGDGQINY 139
PTZ00183 PTZ00183
centrin; Provisional
 2-116 3.18e-11

centrin; Provisional


Pssm-ID: 185503 [Multi-domain]  Cd Length: 158  Bit Score: 57.01  E-value: 3.18e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2064674935   2 DQNRDGFIDKEDLKDTYASLGkTNVKDDELDAML----KEASGPINFTMFLNLFGEKLSGTDAEETILNAFKMLDPDGKG 77
Cdd:PTZ00183   27 DTDGSGTIDPKELKVAMRSLG-FEPKKEEIKQMIadvdKDGSGKIDFEEFLDIMTKKLGERDPREEILKAFRLFDDDKTG 105

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 2064674935  78 KINKEYIKRLLMSQADKMTAEEVDQMFQFASIDVAGNLD 116
Cdd:PTZ00183  106 KISLKNLKRVAKELGETITDEELQEMIDEADRNGDGEIS 144
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
1-114 5.34e-07

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 45.55  E-value: 5.34e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2064674935   1 MDQNRDGFIDKEDLKDTYASLGKTNVKDDELDAmlkeaSGPINFTMFLNlFGEKLSGTDAEETILNAFKMLDPDGKGKIN 80
Cdd:COG5126    14 LDADGDGVLERDDFEALFRRLWATLFSEADTDG-----DGRISREEFVA-GMESLFEATVEPFARAAFDLLDTDGDGKIS 87

                          90       100       110
                  ....*....|....*....|....*....|....
gi 2064674935  81 KEYIKRLLmsQADKMTAEEVDQMfqFASIDVAGN 114
Cdd:COG5126    88 ADEFRRLL--TALGVSEEEADEL--FARLDTDGD 117
EFh_calglandulin_like cd16252
EF-hand, calcium binding motif, found in uncharacterized calglandulin-like proteins; The ...
 40-125 6.90e-07

EF-hand, calcium binding motif, found in uncharacterized calglandulin-like proteins; The family corresponds to a group of uncharacterized calglandulin-like proteins. Although their biological function remain unclear, they show high sequence similarity with human calglandulin-like protein GAGLP, which is an ortholog of calglandulin from the venom glands of Bothrops insularis snake. Both GAGLP and calglandulin are putative Ca2+-binding proteins with four EF-hand motifs. However, members in this family contain only three EF-hand motifs. In this point, they may belong to the parvalbumin-like EF-hand family, which is characterized by the presence of three consecutive EF-hand motifs (helix-loop-helix).


Pssm-ID: 319995 [Multi-domain]  Cd Length: 106  Bit Score: 44.83  E-value: 6.90e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2064674935  40 GPINFTMFLNLFGEKLSGTDAEETILNAFKMLDPDGKGKINKEYIKRLLMSQADKM-----TAEEVDQMFQFASIDVAGN 114
Cdd:cd16252    15 GSFNYSKFFEYMQKFQTSEQQEEAIRKAFQMLDKDKSGFIEWNEIKYILSTVPSSMpvaplSDEEAEAMIQAADTDGDGR 94

                          90
                  ....*....|.
gi 2064674935 115 LDYKALSYVIT 125
Cdd:cd16252    95 IDFQEFSDMVK 105
EF-hand_8 pfam13833
EF-hand domain pair;
6-51 3.70e-04

EF-hand domain pair;


Pssm-ID: 404678 [Multi-domain]  Cd Length: 54  Bit Score: 36.14  E-value: 3.70e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 2064674935   6 DGFIDKEDLKDTYASLGKTNVKDDELDAMLKEA----SGPINFTMFLNLF 51
Cdd:pfam13833   2 KGVITREELKRALALLGLKDLSEDEVDILFREFdtdgDGYISFDEFCVLL 51
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 67-118 5.75e-04

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 35.99  E-value: 5.75e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|..
gi 2064674935  67 AFKMLDPDGKGKINKEYIKRLLMSQADKMTAEEVDQMFQFASIDVAGNLDYK 118
Cdd:cd00051     5 AFRLFDKDGDGTISADELKAALKSLGEGLSEEEIDEMIREVDKDGDGKIDFE 56
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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