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Conserved domains on  [gi|205360949|ref|NP_077282|]
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fatty acid 2-hydroxylase [Homo sapiens]

Protein Classification

fatty acid 2-hydroxylase( domain architecture ID 10445814)

fatty acid 2-hydroxylase is an integral membrane involved in processes such as cholesterol biosynthesis or biosynthesis of plant cuticular wax. It catalyzes the hydroxylation of free fatty acids at the C-2 position to produce 2-hydroxy fatty acids, which are building blocks of sphingolipids and glycosphingolipids, for example, and contains an N-terminal cytochrome b5-like heme/steroid binding domain

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
FA_hydroxylase super family cl01132
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
 124-366 1.55e-86

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


The actual alignment was detected with superfamily member PLN02434:

Pssm-ID: 412761 [Multi-domain]  Cd Length: 237  Bit Score: 261.50  E-value: 1.55e-86
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 205360949 124 VDWRKPLLWQVGHLGEKYDEWVHQPVTR--PIRLFHSDLIEGLSKTVWYSVPIIWVPLVLylsWSYYRTFAQGNvrlfts 201
Cdd:PLN02434   8 VDLNKPLVFQVGHLGEDYEEWVHQPIVSkeGPRFFESDVLEFLTRTVWWAVPLIWLPVVC---WCLVKSVRMGL------ 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 205360949 202 ftteytvavPKSMFPGLFMLGTFLWSLIEYLIHRFLFHMKPpsDSYYLIMLHFVMHGQHHKAPFDGSRLVFPPVPASLVI 281
Cdd:PLN02434  79 ---------PLSAVVLMVAFGVFIWTLLEYILHRFLFHIKT--KSYWGNTAHYLLHGCHHKHPMDGLRLVFPPAATAILC 147

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 205360949 282 GVFYLCMQLILPEAVGGTVFAGGLLGYVLYDMTHYYLHFGSPHKGsYLYSLKAHHVKHHFAHQKSGFGISTKLWDYCFHT 361
Cdd:PLN02434 148 VPFWNLIALFATPATAPALFGGGLLGYVMYDCTHYFLHHGQPSTD-VLRNLKKYHLNHHFRDQDKGFGITSSLWDRVFGT 226


                 ....*
gi 205360949 362 LTPEK 366
Cdd:PLN02434 227 LPPSK 231
Cyt-b5 pfam00173
Cytochrome b5-like Heme/Steroid binding domain; This family includes heme binding domains from ...
 15-86 1.82e-12

Cytochrome b5-like Heme/Steroid binding domain; This family includes heme binding domains from a diverse range of proteins. This family also includes proteins that bind to steroids. The family includes progesterone receptors. Many members of this subfamily are membrane anchored by an N-terminal transmembrane alpha helix. This family also includes a domain in some chitin synthases. There is no known ligand for this domain in the chitin synthases.


:

Pssm-ID: 459698 [Multi-domain]  Cd Length: 74  Bit Score: 62.25  E-value: 1.82e-12
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 205360949   15 EVQRRLAAGACWVRRGARLYDLSSFVRHHPGGEQLLRARAGQDISADLDGPPHRhSANARRWLEQYYVGELR 86
Cdd:pfam00173   4 ELSKHNGDGDCWVAINGKVYDVTKFLKEHPGGEDVILSAAGKDATDAFEAIGHS-EDAAEKLLKKYRIGELA 74
 
Name Accession Description Interval E-value
PLN02434 PLN02434
fatty acid hydroxylase
 124-366 1.55e-86

fatty acid hydroxylase


Pssm-ID: 178053 [Multi-domain]  Cd Length: 237  Bit Score: 261.50  E-value: 1.55e-86
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 205360949 124 VDWRKPLLWQVGHLGEKYDEWVHQPVTR--PIRLFHSDLIEGLSKTVWYSVPIIWVPLVLylsWSYYRTFAQGNvrlfts 201
Cdd:PLN02434   8 VDLNKPLVFQVGHLGEDYEEWVHQPIVSkeGPRFFESDVLEFLTRTVWWAVPLIWLPVVC---WCLVKSVRMGL------ 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 205360949 202 ftteytvavPKSMFPGLFMLGTFLWSLIEYLIHRFLFHMKPpsDSYYLIMLHFVMHGQHHKAPFDGSRLVFPPVPASLVI 281
Cdd:PLN02434  79 ---------PLSAVVLMVAFGVFIWTLLEYILHRFLFHIKT--KSYWGNTAHYLLHGCHHKHPMDGLRLVFPPAATAILC 147

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 205360949 282 GVFYLCMQLILPEAVGGTVFAGGLLGYVLYDMTHYYLHFGSPHKGsYLYSLKAHHVKHHFAHQKSGFGISTKLWDYCFHT 361
Cdd:PLN02434 148 VPFWNLIALFATPATAPALFGGGLLGYVMYDCTHYFLHHGQPSTD-VLRNLKKYHLNHHFRDQDKGFGITSSLWDRVFGT 226


                 ....*
gi 205360949 362 LTPEK 366
Cdd:PLN02434 227 LPPSK 231
FA_hydroxylase pfam04116
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
 219-361 9.98e-18

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


Pssm-ID: 397991  Cd Length: 134  Bit Score: 78.63  E-value: 9.98e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 205360949  219 FMLGTFLWSLIEYLIHRfLFHMKPPsdsyylimLHFVMHGQHH--KAPFDGSRLVFPPVPASLVIGVFYLcmQLILPEAV 296
Cdd:pfam04116   1 VLLGLLLFDFLFYWVHR-LLHRLPW--------LWRRFHKVHHssEAPNALTALRFHPLEALLFALLVLL--PLLLLGLP 69

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 205360949  297 GGTVFAGGLLGYVLYDMTHYYLHFGSPHKGSYLYSLKAHHVKHHFAHQKSGFGISTKLWDYCFHT 361
Cdd:pfam04116  70 LLAFLLGLLLGTLWYLFIHSGLLFPLPWLLKRLLGTPRFHRLHHSKNEEYNFGVTFPLWDRLFGT 134
Cyt-b5 pfam00173
Cytochrome b5-like Heme/Steroid binding domain; This family includes heme binding domains from ...
 15-86 1.82e-12

Cytochrome b5-like Heme/Steroid binding domain; This family includes heme binding domains from a diverse range of proteins. This family also includes proteins that bind to steroids. The family includes progesterone receptors. Many members of this subfamily are membrane anchored by an N-terminal transmembrane alpha helix. This family also includes a domain in some chitin synthases. There is no known ligand for this domain in the chitin synthases.


Pssm-ID: 459698 [Multi-domain]  Cd Length: 74  Bit Score: 62.25  E-value: 1.82e-12
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 205360949   15 EVQRRLAAGACWVRRGARLYDLSSFVRHHPGGEQLLRARAGQDISADLDGPPHRhSANARRWLEQYYVGELR 86
Cdd:pfam00173   4 ELSKHNGDGDCWVAINGKVYDVTKFLKEHPGGEDVILSAAGKDATDAFEAIGHS-EDAAEKLLKKYRIGELA 74
CYB5 COG5274
Cytochrome b involved in lipid metabolism [Energy production and conversion, Lipid transport ...
 25-87 6.25e-11

Cytochrome b involved in lipid metabolism [Energy production and conversion, Lipid transport and metabolism];


Pssm-ID: 444085 [Multi-domain]  Cd Length: 93  Bit Score: 58.51  E-value: 6.25e-11
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 205360949  25 CWVRRGARLYDLSSFVRHHPGGEQLLRARAGQDISADLDGpPHRHSANARRWLEQYYVGELRG 87
Cdd:COG5274   32 CWMAIDGNVYDLTEYIPKHPGGEAVILRWCGKDATEAFNT-KHPHSPKAERLLESYRIGRLAQ 93
PLN02252 PLN02252
nitrate reductase [NADPH]
 15-85 1.08e-07

nitrate reductase [NADPH]


Pssm-ID: 215141 [Multi-domain]  Cd Length: 888  Bit Score: 53.91  E-value: 1.08e-07
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 205360949  15 EVQRRLAAGACWVRRGARLYDLSSFVRHHPGGEQLLRARAGQDISADLDGPphrHSANARRWLEQYYVGEL 85
Cdd:PLN02252 524 EVRKHNSEDSCWIVVHGHVYDCTRFLKDHPGGADSILINAGTDCTEEFDAI---HSDKAKKMLEDYRIGEL 591
 
Name Accession Description Interval E-value
PLN02434 PLN02434
fatty acid hydroxylase
 124-366 1.55e-86

fatty acid hydroxylase


Pssm-ID: 178053 [Multi-domain]  Cd Length: 237  Bit Score: 261.50  E-value: 1.55e-86
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 205360949 124 VDWRKPLLWQVGHLGEKYDEWVHQPVTR--PIRLFHSDLIEGLSKTVWYSVPIIWVPLVLylsWSYYRTFAQGNvrlfts 201
Cdd:PLN02434   8 VDLNKPLVFQVGHLGEDYEEWVHQPIVSkeGPRFFESDVLEFLTRTVWWAVPLIWLPVVC---WCLVKSVRMGL------ 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 205360949 202 ftteytvavPKSMFPGLFMLGTFLWSLIEYLIHRFLFHMKPpsDSYYLIMLHFVMHGQHHKAPFDGSRLVFPPVPASLVI 281
Cdd:PLN02434  79 ---------PLSAVVLMVAFGVFIWTLLEYILHRFLFHIKT--KSYWGNTAHYLLHGCHHKHPMDGLRLVFPPAATAILC 147

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 205360949 282 GVFYLCMQLILPEAVGGTVFAGGLLGYVLYDMTHYYLHFGSPHKGsYLYSLKAHHVKHHFAHQKSGFGISTKLWDYCFHT 361
Cdd:PLN02434 148 VPFWNLIALFATPATAPALFGGGLLGYVMYDCTHYFLHHGQPSTD-VLRNLKKYHLNHHFRDQDKGFGITSSLWDRVFGT 226


                 ....*
gi 205360949 362 LTPEK 366
Cdd:PLN02434 227 LPPSK 231
FA_hydroxylase pfam04116
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
 219-361 9.98e-18

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


Pssm-ID: 397991  Cd Length: 134  Bit Score: 78.63  E-value: 9.98e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 205360949  219 FMLGTFLWSLIEYLIHRfLFHMKPPsdsyylimLHFVMHGQHH--KAPFDGSRLVFPPVPASLVIGVFYLcmQLILPEAV 296
Cdd:pfam04116   1 VLLGLLLFDFLFYWVHR-LLHRLPW--------LWRRFHKVHHssEAPNALTALRFHPLEALLFALLVLL--PLLLLGLP 69

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 205360949  297 GGTVFAGGLLGYVLYDMTHYYLHFGSPHKGSYLYSLKAHHVKHHFAHQKSGFGISTKLWDYCFHT 361
Cdd:pfam04116  70 LLAFLLGLLLGTLWYLFIHSGLLFPLPWLLKRLLGTPRFHRLHHSKNEEYNFGVTFPLWDRLFGT 134
Cyt-b5 pfam00173
Cytochrome b5-like Heme/Steroid binding domain; This family includes heme binding domains from ...
 15-86 1.82e-12

Cytochrome b5-like Heme/Steroid binding domain; This family includes heme binding domains from a diverse range of proteins. This family also includes proteins that bind to steroids. The family includes progesterone receptors. Many members of this subfamily are membrane anchored by an N-terminal transmembrane alpha helix. This family also includes a domain in some chitin synthases. There is no known ligand for this domain in the chitin synthases.


Pssm-ID: 459698 [Multi-domain]  Cd Length: 74  Bit Score: 62.25  E-value: 1.82e-12
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 205360949   15 EVQRRLAAGACWVRRGARLYDLSSFVRHHPGGEQLLRARAGQDISADLDGPPHRhSANARRWLEQYYVGELR 86
Cdd:pfam00173   4 ELSKHNGDGDCWVAINGKVYDVTKFLKEHPGGEDVILSAAGKDATDAFEAIGHS-EDAAEKLLKKYRIGELA 74
CYB5 COG5274
Cytochrome b involved in lipid metabolism [Energy production and conversion, Lipid transport ...
 25-87 6.25e-11

Cytochrome b involved in lipid metabolism [Energy production and conversion, Lipid transport and metabolism];


Pssm-ID: 444085 [Multi-domain]  Cd Length: 93  Bit Score: 58.51  E-value: 6.25e-11
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 205360949  25 CWVRRGARLYDLSSFVRHHPGGEQLLRARAGQDISADLDGpPHRHSANARRWLEQYYVGELRG 87
Cdd:COG5274   32 CWMAIDGNVYDLTEYIPKHPGGEAVILRWCGKDATEAFNT-KHPHSPKAERLLESYRIGRLAQ 93
PLN02252 PLN02252
nitrate reductase [NADPH]
 15-85 1.08e-07

nitrate reductase [NADPH]


Pssm-ID: 215141 [Multi-domain]  Cd Length: 888  Bit Score: 53.91  E-value: 1.08e-07
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 205360949  15 EVQRRLAAGACWVRRGARLYDLSSFVRHHPGGEQLLRARAGQDISADLDGPphrHSANARRWLEQYYVGEL 85
Cdd:PLN02252 524 EVRKHNSEDSCWIVVHGHVYDCTRFLKDHPGGADSILINAGTDCTEEFDAI---HSDKAKKMLEDYRIGEL 591
PLN03198 PLN03198
delta6-acyl-lipid desaturase; Provisional
 25-85 6.36e-03

delta6-acyl-lipid desaturase; Provisional


Pssm-ID: 178739 [Multi-domain]  Cd Length: 526  Bit Score: 38.52  E-value: 6.36e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 205360949  25 CWVRRGARLYDLSSFVRHHPGGEQLLR--ARAGQDISADLdgpphrHSANARRWLEQYYVGEL 85
Cdd:PLN03198 120 CWIVIKNKVYDVSDFAAEHPGGSVISTyfGRDGTDAFSSF------HAASTWKILQDFYIGDV 176
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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