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Conserved domains on  [gi|20071881|gb|AAH26676|]
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Zscan22 protein [Mus musculus]

Protein Classification

C2H2-type zinc finger protein( domain architecture ID 11472214)

Cys2His2 (C2H2)-type zinc finger protein may be involved in transcriptional regulation

CATH:  3.30.160.60
Gene Ontology:  GO:0008270|GO:0003677
PubMed:  11361095|22803940
SCOP:  4003583

Graphical summary

 Zoom to residue level

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List of domain hits

 Name Accession Description Interval E-value
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
19-320 6.54e-09

FOG: Zn-finger [General function prediction only];


:

Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 57.01  E-value: 6.54e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881  19 TCKPESHSESQPELL--GALWMKSTAQEMDFRKALGPHMDAPKDQPGHESNTSGNGSNMWPNFPSQDKASSEEKFGPLLD 96
Cdd:COG5048 137 LPDLLSISNLRNNPLpgNNSSSVNTPQSNSLHPPLPANSLSKDPSSNLSLLISSNVSTSIPSSSENSPLSSSYSIPSSSS 216

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881  97 NETVPPDTCSEKKSSKDSECLKTF--QNTSALEAHQKSHSQKTPYACTECGKVFSRSTHLVQHQVVHTGA-KPHACKECG 173
Cdd:COG5048 217 DQNLENSSSSLPLTTNSQLSPKSLlsQSPSSLSSSDSSSSASESPRSSLPTASSQSSSPNESDSSSEKGFsLPIKSKQCN 296

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881 174 KAFSRVAHLTQHLR--IHTGE--KPYKCEE--CGKTFSRSTHLTQHQRVHTGERPYEC--DTCGKAFSQSTH-----LTQ 240
Cdd:COG5048 297 ISFSRSSPLTRHLRsvNHSGEslKPFSCPYslCGKLFSRNDALKRHILLHTSISPAKEklLNSSSKFSPLLNneppqSLQ 376

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881 241 HQRIHTGEKPYRCDV--CGKAFSDCSALVRHLRVHSGEKPYQCKD--CPKAFAQSSSLIEHQRTHTGEKPYKCSDCGKAF 316
Cdd:COG5048 377 QYKDLKNDKKSETLSnsCIRNFKRDSNLSLHIITHLSFRPYNCKNppCSKSFNRHYNLIPHKKIHTNHAPLLCSILKSFR 456


                ....
gi 20071881 317 SRSS 320
Cdd:COG5048 457 RDLD 460
 
Name Accession Description Interval E-value
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
19-320 6.54e-09

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 57.01  E-value: 6.54e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881  19 TCKPESHSESQPELL--GALWMKSTAQEMDFRKALGPHMDAPKDQPGHESNTSGNGSNMWPNFPSQDKASSEEKFGPLLD 96
Cdd:COG5048 137 LPDLLSISNLRNNPLpgNNSSSVNTPQSNSLHPPLPANSLSKDPSSNLSLLISSNVSTSIPSSSENSPLSSSYSIPSSSS 216

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881  97 NETVPPDTCSEKKSSKDSECLKTF--QNTSALEAHQKSHSQKTPYACTECGKVFSRSTHLVQHQVVHTGA-KPHACKECG 173
Cdd:COG5048 217 DQNLENSSSSLPLTTNSQLSPKSLlsQSPSSLSSSDSSSSASESPRSSLPTASSQSSSPNESDSSSEKGFsLPIKSKQCN 296

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881 174 KAFSRVAHLTQHLR--IHTGE--KPYKCEE--CGKTFSRSTHLTQHQRVHTGERPYEC--DTCGKAFSQSTH-----LTQ 240
Cdd:COG5048 297 ISFSRSSPLTRHLRsvNHSGEslKPFSCPYslCGKLFSRNDALKRHILLHTSISPAKEklLNSSSKFSPLLNneppqSLQ 376

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881 241 HQRIHTGEKPYRCDV--CGKAFSDCSALVRHLRVHSGEKPYQCKD--CPKAFAQSSSLIEHQRTHTGEKPYKCSDCGKAF 316
Cdd:COG5048 377 QYKDLKNDKKSETLSnsCIRNFKRDSNLSLHIITHLSFRPYNCKNppCSKSFNRHYNLIPHKKIHTNHAPLLCSILKSFR 456


                ....
gi 20071881 317 SRSS 320
Cdd:COG5048 457 RDLD 460
zf-H2C2_2 pfam13465
Zinc-finger double domain;
181-206 2.67e-05

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 40.43  E-value: 2.67e-05
                          10        20
                  ....*....|....*....|....*.
gi 20071881   181 HLTQHLRIHTGEKPYKCEECGKTFSR 206
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
SUF4-like cd20908
N-terminal domain of Oryza sativa transcription factor SUPPRESSOR OF FRI 4 (OsSUF4), ...
 228-273 6.08e-04

N-terminal domain of Oryza sativa transcription factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana SUF4 (AtSUF4), and similar proteins; Oryza sativa SUPPRESSOR OF FRI 4 (OsSUF4) is a C2H2-type zinc finger transcription factor which interacts with the major H3K36 methyltransferase SDG725 to promote H3K36me3 (tri-methylation at H3K9) establishment. The transcription factor OsSUF4 recognizes a specific 7-bp DNA element (5'-CGGAAAT-3'), which is contained in the promoter regions of many genes throughout the rice genome. Through interaction with OsSUF4, SDG725 is recruited to the promoters of key florigen genes, RICE FLOWERING LOCUS T1 (RFT1) and Heading date 3a (Hd3a), for H3K36 deposition to promote gene activation and rice plant flowering. OsSUF4 target genes include a number of genes involved in many biological processes. Flowering plant Arabidopsis SUF4 binds to a 15bp DNA element (5'-CCAAATTTTAAGTTT-3') within the promoter of the floral repressor gene FLOWERING LOCUS C (FLC) and recruits the FRI-C transcription activator complex to the FLC promoter. Although the DNA-binding element and target genes of AtSUF4 are different from those of OsSUF4, AtSUF4 is known to interact with the Arabidopsis H3K36 methyltransferase SDG8 (also known as ASHH2/EFS/SET8), and the methylation deposition mechanism mediated by the SUF4 transcription factor and H3K36 methyltransferase may be conserved in Arabidopsis and rice. Proteins in this family have two conserved C2H2-type zinc finger motifs at the N-terminus (included in this model), and a large proline-rich domain at the C-terminus; for OsSUF4, it has been shown that the N-terminal zinc-finger domain is responsible for DNA binding, and that the C-terminal domain interacts with SDG725.


Pssm-ID: 411020 [Multi-domain]  Cd Length: 82  Bit Score: 37.92  E-value: 6.08e-04
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*..
gi 20071881 228 CGKAFSQSTHLTQHQRIHTgekpYRCDVCGKAFSDCSALVRH-LRVH 273
Cdd:cd20908   7 CDREFDDEKILIQHQKAKH----FKCHICHKKLYTAGGLAVHcLQVH 49
 
Name Accession Description Interval E-value
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
19-320 6.54e-09

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 57.01  E-value: 6.54e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881  19 TCKPESHSESQPELL--GALWMKSTAQEMDFRKALGPHMDAPKDQPGHESNTSGNGSNMWPNFPSQDKASSEEKFGPLLD 96
Cdd:COG5048 137 LPDLLSISNLRNNPLpgNNSSSVNTPQSNSLHPPLPANSLSKDPSSNLSLLISSNVSTSIPSSSENSPLSSSYSIPSSSS 216

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881  97 NETVPPDTCSEKKSSKDSECLKTF--QNTSALEAHQKSHSQKTPYACTECGKVFSRSTHLVQHQVVHTGA-KPHACKECG 173
Cdd:COG5048 217 DQNLENSSSSLPLTTNSQLSPKSLlsQSPSSLSSSDSSSSASESPRSSLPTASSQSSSPNESDSSSEKGFsLPIKSKQCN 296

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881 174 KAFSRVAHLTQHLR--IHTGE--KPYKCEE--CGKTFSRSTHLTQHQRVHTGERPYEC--DTCGKAFSQSTH-----LTQ 240
Cdd:COG5048 297 ISFSRSSPLTRHLRsvNHSGEslKPFSCPYslCGKLFSRNDALKRHILLHTSISPAKEklLNSSSKFSPLLNneppqSLQ 376

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881 241 HQRIHTGEKPYRCDV--CGKAFSDCSALVRHLRVHSGEKPYQCKD--CPKAFAQSSSLIEHQRTHTGEKPYKCSDCGKAF 316
Cdd:COG5048 377 QYKDLKNDKKSETLSnsCIRNFKRDSNLSLHIITHLSFRPYNCKNppCSKSFNRHYNLIPHKKIHTNHAPLLCSILKSFR 456


                ....
gi 20071881 317 SRSS 320
Cdd:COG5048 457 RDLD 460
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
193-335 2.04e-07

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 52.39  E-value: 2.04e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881 193 KPYKCEECGKTFSRSTHLTQHQR--VHTGE--RPYECD--TCGKAFSQSTHLTQHQRIHTGEKPYRC--DVCGKAFSDCS 264
Cdd:COG5048 288 LPIKSKQCNISFSRSSPLTRHLRsvNHSGEslKPFSCPysLCGKLFSRNDALKRHILLHTSISPAKEklLNSSSKFSPLL 367

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881 265 ALVRHLRVH-----SGEKPYQC--KDCPKAFAQSSSLIEHQRTHTGEKP--YKCSDCGKAFSRSSALMVHLKIHITVTRS 335
Cdd:COG5048 368 NNEPPQSLQqykdlKNDKKSETlsNSCIRNFKRDSNLSLHIITHLSFRPynCKNPPCSKSFNRHYNLIPHKKIHTNHAPL 447
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
221-301 1.47e-05

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 46.61  E-value: 1.47e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881 221 RPYECDTCGKAFSQSTHLTQHQRIHTGEKPYRCDV--CGKAFSDCSALVRHLRVHSGEKPYQCKD--CPKAFAQSSSLIE 296
Cdd:COG5048  32 RPDSCPNCTDSFSRLEHLTRHIRSHTGEKPSQCSYsgCDKSFSRPLELSRHLRTHHNNPSDLNSKslPLSNSKASSSSLS 111


                ....*
gi 20071881 297 HQRTH 301
Cdd:COG5048 112 SSSSN 116
zf-H2C2_2 pfam13465
Zinc-finger double domain;
181-206 2.67e-05

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 40.43  E-value: 2.67e-05
                          10        20
                  ....*....|....*....|....*.
gi 20071881   181 HLTQHLRIHTGEKPYKCEECGKTFSR 206
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
zf-H2C2_2 pfam13465
Zinc-finger double domain;
237-261 6.52e-05

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 39.28  E-value: 6.52e-05
                          10        20
                  ....*....|....*....|....*
gi 20071881   237 HLTQHQRIHTGEKPYRCDVCGKAFS 261
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFK 25
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
14-272 7.41e-05

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 44.30  E-value: 7.41e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881  14 STLAHTCKPESHSESQPELLGALWMKSTAQEMDFrkaLGPHMDAPKDQPGHESNTSGNGSNMWPNFPSQDKASSEEKFGP 93
Cdd:COG5048 195 SIPSSSENSPLSSSYSIPSSSSDQNLENSSSSLP---LTTNSQLSPKSLLSQSPSSLSSSDSSSSASESPRSSLPTASSQ 271

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881  94 LL-DNETVPPDTCSEKKSSKDSECLKTFQNTSALEAHQKSHSQK----TPYACTE--CGKVFSRSTHLVQHQVVHTGAKP 166
Cdd:COG5048 272 SSsPNESDSSSEKGFSLPIKSKQCNISFSRSSPLTRHLRSVNHSgeslKPFSCPYslCGKLFSRNDALKRHILLHTSISP 351

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881 167 HACK--ECGKAFS------RVAHLTQHLRIHTGEKPYKCE-ECGKTFSRSTHLTQHQRVHTGERPYECD--TCGKAFSQS 235
Cdd:COG5048 352 AKEKllNSSSKFSpllnnePPQSLQQYKDLKNDKKSETLSnSCIRNFKRDSNLSLHIITHLSFRPYNCKnpPCSKSFNRH 431

                       250       260       270
                ....*....|....*....|....*....|....*..
gi 20071881 236 THLTQHQRIHTGEKPYRCDVCGKaFSDCSALVRHLRV 272
Cdd:COG5048 432 YNLIPHKKIHTNHAPLLCSILKS-FRRDLDLSNHGKD 467
zf-H2C2_2 pfam13465
Zinc-finger double domain;
293-318 1.39e-04

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 38.51  E-value: 1.39e-04
                          10        20
                  ....*....|....*....|....*.
gi 20071881   293 SLIEHQRTHTGEKPYKCSDCGKAFSR 318
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
SFP1 COG5189
Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division ...
 191-274 2.06e-04

Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division and chromosome partitioning];


Pssm-ID: 227516 [Multi-domain]  Cd Length: 423  Bit Score: 42.78  E-value: 2.06e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881 191 GEKPYKCE--ECGKTFSRSTHLTQHqRVHTGERPYECDTCGKafsqsthlTQHQRIHTGEKPYRCDVCGKAFSDCSALVR 268
Cdd:COG5189 346 DGKPYKCPveGCNKKYKNQNGLKYH-MLHGHQNQKLHENPSP--------EKMNIFSAKDKPYRCEVCDKRYKNLNGLKY 416


                ....*.
gi 20071881 269 HlRVHS 274
Cdd:COG5189 417 H-RKHS 421
zf-H2C2_2 pfam13465
Zinc-finger double domain;
209-234 2.16e-04

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 37.74  E-value: 2.16e-04
                          10        20
                  ....*....|....*....|....*.
gi 20071881   209 HLTQHQRVHTGERPYECDTCGKAFSQ 234
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
zf-H2C2_2 pfam13465
Zinc-finger double domain;
265-290 2.22e-04

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 37.74  E-value: 2.22e-04
                          10        20
                  ....*....|....*....|....*.
gi 20071881   265 ALVRHLRVHSGEKPYQCKDCPKAFAQ 290
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 195-217 3.39e-04

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 37.28  E-value: 3.39e-04
                          10        20
                  ....*....|....*....|...
gi 20071881   195 YKCEECGKTFSRSTHLTQHQRVH 217
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
193-254 4.65e-04

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 41.61  E-value: 4.65e-04
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 20071881 193 KPYKCEECGKTFSRSTHLTQHQRVHTGERPYEC--DTCGKAFSQSTHLTQHQRIHTGEKPYRCD 254
Cdd:COG5048  32 RPDSCPNCTDSFSRLEHLTRHIRSHTGEKPSQCsySGCDKSFSRPLELSRHLRTHHNNPSDLNS 95
SUF4-like cd20908
N-terminal domain of Oryza sativa transcription factor SUPPRESSOR OF FRI 4 (OsSUF4), ...
 228-273 6.08e-04

N-terminal domain of Oryza sativa transcription factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana SUF4 (AtSUF4), and similar proteins; Oryza sativa SUPPRESSOR OF FRI 4 (OsSUF4) is a C2H2-type zinc finger transcription factor which interacts with the major H3K36 methyltransferase SDG725 to promote H3K36me3 (tri-methylation at H3K9) establishment. The transcription factor OsSUF4 recognizes a specific 7-bp DNA element (5'-CGGAAAT-3'), which is contained in the promoter regions of many genes throughout the rice genome. Through interaction with OsSUF4, SDG725 is recruited to the promoters of key florigen genes, RICE FLOWERING LOCUS T1 (RFT1) and Heading date 3a (Hd3a), for H3K36 deposition to promote gene activation and rice plant flowering. OsSUF4 target genes include a number of genes involved in many biological processes. Flowering plant Arabidopsis SUF4 binds to a 15bp DNA element (5'-CCAAATTTTAAGTTT-3') within the promoter of the floral repressor gene FLOWERING LOCUS C (FLC) and recruits the FRI-C transcription activator complex to the FLC promoter. Although the DNA-binding element and target genes of AtSUF4 are different from those of OsSUF4, AtSUF4 is known to interact with the Arabidopsis H3K36 methyltransferase SDG8 (also known as ASHH2/EFS/SET8), and the methylation deposition mechanism mediated by the SUF4 transcription factor and H3K36 methyltransferase may be conserved in Arabidopsis and rice. Proteins in this family have two conserved C2H2-type zinc finger motifs at the N-terminus (included in this model), and a large proline-rich domain at the C-terminus; for OsSUF4, it has been shown that the N-terminal zinc-finger domain is responsible for DNA binding, and that the C-terminal domain interacts with SDG725.


Pssm-ID: 411020 [Multi-domain]  Cd Length: 82  Bit Score: 37.92  E-value: 6.08e-04
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*..
gi 20071881 228 CGKAFSQSTHLTQHQRIHTgekpYRCDVCGKAFSDCSALVRH-LRVH 273
Cdd:cd20908   7 CDREFDDEKILIQHQKAKH----FKCHICHKKLYTAGGLAVHcLQVH 49
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
165-236 7.10e-04

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 41.22  E-value: 7.10e-04
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 20071881 165 KPHACKECGKAFSRVAHLTQHLRIHTGEKPYKC--EECGKTFSRSTHLTQHQRVHTGERPYECDTCGKAFSQST 236
Cdd:COG5048  32 RPDSCPNCTDSFSRLEHLTRHIRSHTGEKPSQCsySGCDKSFSRPLELSRHLRTHHNNPSDLNSKSLPLSNSKA 105
SUF4-like cd20908
N-terminal domain of Oryza sativa transcription factor SUPPRESSOR OF FRI 4 (OsSUF4), ...
 277-329 8.49e-04

N-terminal domain of Oryza sativa transcription factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana SUF4 (AtSUF4), and similar proteins; Oryza sativa SUPPRESSOR OF FRI 4 (OsSUF4) is a C2H2-type zinc finger transcription factor which interacts with the major H3K36 methyltransferase SDG725 to promote H3K36me3 (tri-methylation at H3K9) establishment. The transcription factor OsSUF4 recognizes a specific 7-bp DNA element (5'-CGGAAAT-3'), which is contained in the promoter regions of many genes throughout the rice genome. Through interaction with OsSUF4, SDG725 is recruited to the promoters of key florigen genes, RICE FLOWERING LOCUS T1 (RFT1) and Heading date 3a (Hd3a), for H3K36 deposition to promote gene activation and rice plant flowering. OsSUF4 target genes include a number of genes involved in many biological processes. Flowering plant Arabidopsis SUF4 binds to a 15bp DNA element (5'-CCAAATTTTAAGTTT-3') within the promoter of the floral repressor gene FLOWERING LOCUS C (FLC) and recruits the FRI-C transcription activator complex to the FLC promoter. Although the DNA-binding element and target genes of AtSUF4 are different from those of OsSUF4, AtSUF4 is known to interact with the Arabidopsis H3K36 methyltransferase SDG8 (also known as ASHH2/EFS/SET8), and the methylation deposition mechanism mediated by the SUF4 transcription factor and H3K36 methyltransferase may be conserved in Arabidopsis and rice. Proteins in this family have two conserved C2H2-type zinc finger motifs at the N-terminus (included in this model), and a large proline-rich domain at the C-terminus; for OsSUF4, it has been shown that the N-terminal zinc-finger domain is responsible for DNA binding, and that the C-terminal domain interacts with SDG725.


Pssm-ID: 411020 [Multi-domain]  Cd Length: 82  Bit Score: 37.54  E-value: 8.49e-04
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....
gi 20071881 277 KPYqCKDCPKAFAQSSSLIEHQRTHTgekpYKCSDCGKAFSRSSALMVH-LKIH 329
Cdd:cd20908   1 KPW-CYYCDREFDDEKILIQHQKAKH----FKCHICHKKLYTAGGLAVHcLQVH 49
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 307-329 1.71e-03

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 35.35  E-value: 1.71e-03
                          10        20
                  ....*....|....*....|...
gi 20071881   307 YKCSDCGKAFSRSSALMVHLKIH 329
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 279-301 2.43e-03

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 34.97  E-value: 2.43e-03
                          10        20
                  ....*....|....*....|...
gi 20071881   279 YQCKDCPKAFAQSSSLIEHQRTH 301
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 223-245 2.84e-03

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 34.58  E-value: 2.84e-03
                          10        20
                  ....*....|....*....|...
gi 20071881   223 YECDTCGKAFSQSTHLTQHQRIH 245
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
249-314 3.06e-03

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 39.29  E-value: 3.06e-03
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 20071881 249 KPYRCDVCGKAFSDCSALVRHLRVHSGEKPYQCKDCPKAFAQS--SSLIEHQRTHTGEKPYKCSDCGK 314
Cdd:COG5048  32 RPDSCPNCTDSFSRLEHLTRHIRSHTGEKPSQCSYSGCDKSFSrpLELSRHLRTHHNNPSDLNSKSLP 99
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
110-190 3.14e-03

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 39.29  E-value: 3.14e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881 110 SSKDSECLKTFQNTSALEAHQKSHSQKTPYACTECGKVFSRSTHLVQHQVVHTGAKPHAC--KECGKAFSRVAHLTQHLR 187
Cdd:COG5048   5 SSQSSSSNNSVLSSTPKSTLKSLSNAPRPDSCPNCTDSFSRLEHLTRHIRSHTGEKPSQCsySGCDKSFSRPLELSRHLR 84


                ...
gi 20071881 188 IHT 190
Cdd:COG5048  85 THH 87
SUF4-like cd20908
N-terminal domain of Oryza sativa transcription factor SUPPRESSOR OF FRI 4 (OsSUF4), ...
 169-217 3.48e-03

N-terminal domain of Oryza sativa transcription factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana SUF4 (AtSUF4), and similar proteins; Oryza sativa SUPPRESSOR OF FRI 4 (OsSUF4) is a C2H2-type zinc finger transcription factor which interacts with the major H3K36 methyltransferase SDG725 to promote H3K36me3 (tri-methylation at H3K9) establishment. The transcription factor OsSUF4 recognizes a specific 7-bp DNA element (5'-CGGAAAT-3'), which is contained in the promoter regions of many genes throughout the rice genome. Through interaction with OsSUF4, SDG725 is recruited to the promoters of key florigen genes, RICE FLOWERING LOCUS T1 (RFT1) and Heading date 3a (Hd3a), for H3K36 deposition to promote gene activation and rice plant flowering. OsSUF4 target genes include a number of genes involved in many biological processes. Flowering plant Arabidopsis SUF4 binds to a 15bp DNA element (5'-CCAAATTTTAAGTTT-3') within the promoter of the floral repressor gene FLOWERING LOCUS C (FLC) and recruits the FRI-C transcription activator complex to the FLC promoter. Although the DNA-binding element and target genes of AtSUF4 are different from those of OsSUF4, AtSUF4 is known to interact with the Arabidopsis H3K36 methyltransferase SDG8 (also known as ASHH2/EFS/SET8), and the methylation deposition mechanism mediated by the SUF4 transcription factor and H3K36 methyltransferase may be conserved in Arabidopsis and rice. Proteins in this family have two conserved C2H2-type zinc finger motifs at the N-terminus (included in this model), and a large proline-rich domain at the C-terminus; for OsSUF4, it has been shown that the N-terminal zinc-finger domain is responsible for DNA binding, and that the C-terminal domain interacts with SDG725.


Pssm-ID: 411020 [Multi-domain]  Cd Length: 82  Bit Score: 36.00  E-value: 3.48e-03
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|
gi 20071881 169 CKECGKAFSRVAHLTQHLRIHTgekpYKCEECGKTFSRSTHLTQH-QRVH 217
Cdd:cd20908   4 CYYCDREFDDEKILIQHQKAKH----FKCHICHKKLYTAGGLAVHcLQVH 49
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 251-273 4.33e-03

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 34.20  E-value: 4.33e-03
                          10        20
                  ....*....|....*....|...
gi 20071881   251 YRCDVCGKAFSDCSALVRHLRVH 273
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
66-309 6.75e-03

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 38.14  E-value: 6.75e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881  66 SNTSGNGSNMWPNFPSQDkasseekFGPLLDNETVPPDTCSEKKSSKDSECLKTFQNTSALEAHQKSHSQK-TPYACTEC 144
Cdd:COG5048 106 SSSSLSSSSSNSNDNNLL-------SSHSLPPSSRDPQLPDLLSISNLRNNPLPGNNSSSVNTPQSNSLHPpLPANSLSK 178

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881 145 GKVFSRSTHLvqHQVVHTGAKPHACKECGKAFSRVAHLTQHLRIHTGEKPYKCEECgkTFSRSTHLTQHQRVHTGER--P 222
Cdd:COG5048 179 DPSSNLSLLI--SSNVSTSIPSSSENSPLSSSYSIPSSSSDQNLENSSSSLPLTTN--SQLSPKSLLSQSPSSLSSSdsS 254

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881 223 YECDTCGKAFSQSTHLTQHQRIHTGE-------KPYRCDVCGKAFSDCSALVRHLR--VHSGE--KPYQC--KDCPKAFA 289
Cdd:COG5048 255 SSASESPRSSLPTASSQSSSPNESDSssekgfsLPIKSKQCNISFSRSSPLTRHLRsvNHSGEslKPFSCpySLCGKLFS 334

                       250       260
                ....*....|....*....|
gi 20071881 290 QSSSLIEHQRTHTGEKPYKC 309
Cdd:COG5048 335 RNDALKRHILLHTSISPAKE 354
SFP1 COG5189
Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division ...
 247-327 8.32e-03

Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division and chromosome partitioning];


Pssm-ID: 227516 [Multi-domain]  Cd Length: 423  Bit Score: 37.78  E-value: 8.32e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 20071881 247 GEKPYRCDV--CGKAFSDCSALVRHlRVHSgekpyqckDCPKAFAQSSSLIEHQRTHTGEKPYKCSDCGKAFSRSSALMV 324
Cdd:COG5189 346 DGKPYKCPVegCNKKYKNQNGLKYH-MLHG--------HQNQKLHENPSPEKMNIFSAKDKPYRCEVCDKRYKNLNGLKY 416


                ...
gi 20071881 325 HLK 327
Cdd:COG5189 417 HRK 419
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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