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Conserved domains on  [gi|1970712122|gb|QRD20813|]
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IS3 family transposase [Escherichia coli]

Protein Classification

transposase( domain architecture ID 1000502)

transposase binds to the end of a transposon and catalyzes the movement of the transposon to another part of the genome by a cut and paste mechanism or a replicative transposition mechanism

Gene Ontology:  GO:0004803

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
DDE_3 super family cl46863
DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are ...
 21-109 4.71e-43

DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are probably endonucleases of the DDE superfamily. Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction.


The actual alignment was detected with superfamily member PHA02517:

Pssm-ID: 481203 [Multi-domain]  Cd Length: 277  Bit Score: 141.54  E-value: 4.71e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1970712122  21 LHIAPSTYYHCQQQRHHPDKRSARAQHD--VKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVL 98
Cdd:PHA02517    1 LGIAPSTYYRCQQQRHHPDKRRARAQHDdwLKSEILRVYDENHQVYGVRKVWRQLNREGIRVARCTVGRLMKELGLAGVL 80

                          90
                  ....*....|.
gi 1970712122  99 RGKKVRTTISR 109
Cdd:PHA02517   81 RGKKVRTTISR 91
 
Name Accession Description Interval E-value
PHA02517 PHA02517
putative transposase OrfB; Reviewed
 21-109 4.71e-43

putative transposase OrfB; Reviewed


Pssm-ID: 222853 [Multi-domain]  Cd Length: 277  Bit Score: 141.54  E-value: 4.71e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1970712122  21 LHIAPSTYYHCQQQRHHPDKRSARAQHD--VKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVL 98
Cdd:PHA02517    1 LGIAPSTYYRCQQQRHHPDKRRARAQHDdwLKSEILRVYDENHQVYGVRKVWRQLNREGIRVARCTVGRLMKELGLAGVL 80

                          90
                  ....*....|.
gi 1970712122  99 RGKKVRTTISR 109
Cdd:PHA02517   81 RGKKVRTTISR 91
transpos_IS3 NF033516
IS3 family transposase;
4-110 1.81e-23

IS3 family transposase;


Pssm-ID: 468052 [Multi-domain]  Cd Length: 369  Bit Score: 92.24  E-value: 1.81e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1970712122   4 LLDKLREQYGVGPVCSELHIAPSTYYhcqQQRHHPDKRSARAQHDVKREIQRVYDENHQVYGVRKVWRQLLREGIRVARC 83
Cdd:NF033516   93 LIDALRGEYSVRRACRVLGVSRSTYY---YWRKRPPSRRAPDDAELRARIREIFEESRGRYGYRRITALLRREGIRVNHK 169

                          90       100
                  ....*....|....*....|....*...
gi 1970712122  84 TVARLMAVMGLAGVLRGK-KVRTTISRR 110
Cdd:NF033516  170 RVYRLMRELGLLARRRRKrRPYTTDSGH 197
Tra5 COG2801
Transposase InsO and inactivated derivatives [Mobilome: prophages, transposons];
20-110 2.07e-13

Transposase InsO and inactivated derivatives [Mobilome: prophages, transposons];


Pssm-ID: 442053 [Multi-domain]  Cd Length: 309  Bit Score: 64.02  E-value: 2.07e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1970712122  20 ELHIAPSTYYHCQQQRHHPDKRSARAQHDVKREIQRVYDEnHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLR 99
Cdd:COG2801    44 RLLRRRRARSRRRRRLRRPRSYRADEDAELLERIKEIFAE-SPRYGYRRITAELRREGIAVNRKRVRRLMRELGLQARRR 122

                          90
                  ....*....|.
gi 1970712122 100 GKKVRTTISRR 110
Cdd:COG2801   123 RKKKYTTYSGH 133
HTH_21 pfam13276
HTH-like domain; This domain contains a predicted helix-turn-helix suggesting a DNA-binding ...
 50-102 2.98e-11

HTH-like domain; This domain contains a predicted helix-turn-helix suggesting a DNA-binding function.


Pssm-ID: 463824 [Multi-domain]  Cd Length: 60  Bit Score: 54.11  E-value: 2.98e-11
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1970712122  50 KREIQRVYDENHQVYGVRKVWRQLLREG-IRVARCTVARLMAVMGLAGVLRGKK 102
Cdd:pfam13276   7 LEAIREIFEESRGTYGYRRITAELRREGgIRVNRKRVARLMRELGLRARRRRKR 60
 
Name Accession Description Interval E-value
PHA02517 PHA02517
putative transposase OrfB; Reviewed
 21-109 4.71e-43

putative transposase OrfB; Reviewed


Pssm-ID: 222853 [Multi-domain]  Cd Length: 277  Bit Score: 141.54  E-value: 4.71e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1970712122  21 LHIAPSTYYHCQQQRHHPDKRSARAQHD--VKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVL 98
Cdd:PHA02517    1 LGIAPSTYYRCQQQRHHPDKRRARAQHDdwLKSEILRVYDENHQVYGVRKVWRQLNREGIRVARCTVGRLMKELGLAGVL 80

                          90
                  ....*....|.
gi 1970712122  99 RGKKVRTTISR 109
Cdd:PHA02517   81 RGKKVRTTISR 91
transpos_IS3 NF033516
IS3 family transposase;
4-110 1.81e-23

IS3 family transposase;


Pssm-ID: 468052 [Multi-domain]  Cd Length: 369  Bit Score: 92.24  E-value: 1.81e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1970712122   4 LLDKLREQYGVGPVCSELHIAPSTYYhcqQQRHHPDKRSARAQHDVKREIQRVYDENHQVYGVRKVWRQLLREGIRVARC 83
Cdd:NF033516   93 LIDALRGEYSVRRACRVLGVSRSTYY---YWRKRPPSRRAPDDAELRARIREIFEESRGRYGYRRITALLRREGIRVNHK 169

                          90       100
                  ....*....|....*....|....*...
gi 1970712122  84 TVARLMAVMGLAGVLRGK-KVRTTISRR 110
Cdd:NF033516  170 RVYRLMRELGLLARRRRKrRPYTTDSGH 197
Tra5 COG2801
Transposase InsO and inactivated derivatives [Mobilome: prophages, transposons];
20-110 2.07e-13

Transposase InsO and inactivated derivatives [Mobilome: prophages, transposons];


Pssm-ID: 442053 [Multi-domain]  Cd Length: 309  Bit Score: 64.02  E-value: 2.07e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1970712122  20 ELHIAPSTYYHCQQQRHHPDKRSARAQHDVKREIQRVYDEnHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLR 99
Cdd:COG2801    44 RLLRRRRARSRRRRRLRRPRSYRADEDAELLERIKEIFAE-SPRYGYRRITAELRREGIAVNRKRVRRLMRELGLQARRR 122

                          90
                  ....*....|.
gi 1970712122 100 GKKVRTTISRR 110
Cdd:COG2801   123 RKKKYTTYSGH 133
HTH_21 pfam13276
HTH-like domain; This domain contains a predicted helix-turn-helix suggesting a DNA-binding ...
 50-102 2.98e-11

HTH-like domain; This domain contains a predicted helix-turn-helix suggesting a DNA-binding function.


Pssm-ID: 463824 [Multi-domain]  Cd Length: 60  Bit Score: 54.11  E-value: 2.98e-11
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1970712122  50 KREIQRVYDENHQVYGVRKVWRQLLREG-IRVARCTVARLMAVMGLAGVLRGKK 102
Cdd:pfam13276   7 LEAIREIFEESRGTYGYRRITAELRREGgIRVNRKRVARLMRELGLRARRRRKR 60
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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