forkhead associated (FHA) domain found in kinesin-like protein KIF1C; KIF1C is a new kinesin-like protein involved in vesicle transport from the Golgi apparatus to the endoplasmic reticulum. It has a microtubule plus end-directed motility. The FHA domain is a small phosphopeptide recognition module, but this group may lack the conserved residues that are required for binding phosphothreonine.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907080148  13 TPHLVNLNEDPLMSECLLYHIKDGVTRVGQVDVDIKLTGQFIREQHCLFRSIPQPDGEVMVTLEPCEGAETYVNGKLVTE 92
Cdd:cd22728     1 TPHLVNLNEDPLMSECLLYHIKDGVTRVGQVDVDIKLSGQFIREQHCLFRSIPNPSGEVVVTLEPCEGAETYVNGKQVTE 80

                          90       100
                  ....*....|....*....|..
gi 1907080148  93 PLVLKSGNRIVMGKNHVFRFNH 114
Cdd:cd22728    81 PLVLKSGNRIVMGKNHVFRFNH 102

forkhead associated (FHA) domain found in kinesin-like protein KIF1C; KIF1C is a new kinesin-like protein involved in vesicle transport from the Golgi apparatus to the endoplasmic reticulum. It has a microtubule plus end-directed motility. The FHA domain is a small phosphopeptide recognition module, but this group may lack the conserved residues that are required for binding phosphothreonine.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907080148  13 TPHLVNLNEDPLMSECLLYHIKDGVTRVGQVDVDIKLTGQFIREQHCLFRSIPQPDGEVMVTLEPCEGAETYVNGKLVTE 92
Cdd:cd22728     1 TPHLVNLNEDPLMSECLLYHIKDGVTRVGQVDVDIKLSGQFIREQHCLFRSIPNPSGEVVVTLEPCEGAETYVNGKQVTE 80

                          90       100
                  ....*....|....*....|..
gi 1907080148  93 PLVLKSGNRIVMGKNHVFRFNH 114
Cdd:cd22728    81 PLVLKSGNRIVMGKNHVFRFNH 102

Forkhead associated (FHA) domain, binds pSer, pThr, pTyr [Signal transduction mechanisms];

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907080148  31 YHIKDGVTRVG-QVDVDIKLTGQFIREQHCLFRsiPQPDGevmVTLEPCEGA-ETYVNGKLVTEPLVLKSGNRIVMGKnH 108
Cdd:COG1716    16 FPLDGGPLTIGrAPDNDIVLDDPTVSRRHARIR--RDGGG---WVLEDLGSTnGTFVNGQRVTEPAPLRDGDVIRLGK-T 89

                  ....
gi 1907080148 109 VFRF 112
Cdd:COG1716    90 ELRF 93

forkhead associated (FHA) domain found in kinesin-like protein KIF1C; KIF1C is a new kinesin-like protein involved in vesicle transport from the Golgi apparatus to the endoplasmic reticulum. It has a microtubule plus end-directed motility. The FHA domain is a small phosphopeptide recognition module, but this group may lack the conserved residues that are required for binding phosphothreonine.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907080148  13 TPHLVNLNEDPLMSECLLYHIKDGVTRVGQVDVDIKLTGQFIREQHCLFRSIPQPDGEVMVTLEPCEGAETYVNGKLVTE 92
Cdd:cd22728     1 TPHLVNLNEDPLMSECLLYHIKDGVTRVGQVDVDIKLSGQFIREQHCLFRSIPNPSGEVVVTLEPCEGAETYVNGKQVTE 80

                          90       100
                  ....*....|....*....|..
gi 1907080148  93 PLVLKSGNRIVMGKNHVFRFNH 114
Cdd:cd22728    81 PLVLKSGNRIVMGKNHVFRFNH 102

forkhead associated (FHA) domain found in kinesin-like protein KIF1B; KIF1B, also called Klp, is a motor for anterograde transport of mitochondria. It has a microtubule plus end-directed motility. Isoform 1 mediates the transport of synaptic vesicles in neuronal cells, while isoform 2 is required for induction of neuronal apoptosis. The FHA domain is a small phosphopeptide recognition module, but this group may lack the conserved residues that are required for binding phosphothreonine.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907080148  12 KTPHLVNLNEDPLMSECLLYHIKDGVTRVGQVDV----DIKLTGQFIREQHCLFRSIPQPDGEVMVTLEPCEGAETYVNG 87
Cdd:cd22727     1 KTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAerrqDIVLSGAHIKEEHCIFRSERNNNGEVIVTLEPCERSETYVNG 80

                          90       100       110
                  ....*....|....*....|....*....|
gi 1907080148  88 KLVTEPLVLKSGNRIVMGKNHVFRFNHPEQ 117
Cdd:cd22727    81 KRVVQPVQLRSGNRIIMGKNHVFRFNHPEQ 110

forkhead associated (FHA) domain found in kinesin-like protein KIF14 and similar proteins; KIF14 is a microtubule motor protein that binds to microtubules with high affinity through each tubulin heterodimer and has an ATPase activity. It plays a role in many processes like cell division, cytokinesis and in cell proliferation and apoptosis. KIF14 is a potential oncogene and is involved in the metastasis of various cancers. Mutations of KIF14 cause primary microcephaly by impairing cytokinesis. The FHA domain is a small phosphopeptide recognition module, but this group may lack the conserved residues that are required for binding phosphothreonine.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907080148  12 KTPHLVNLNEDPLMSECLLYHIKDGVTRVGQ----VDVDIKLTGQFIREQHCLFRSIpqpdgEVMVTLEPCEGAETYVNG 87
Cdd:cd22707     6 KLPNLVNLNEDPQLSEMLLYMLKEGQTRVGRskasSSHDIQLSGALIADDHCTIENN-----GGKVTIIPVGDAETYVNG 80

                          90       100
                  ....*....|....*....|....*...
gi 1907080148  88 KLVTEPLVLKSGNRIVMGKNHVFRFNHP 115
Cdd:cd22707    81 ELISEPTVLHHGDRVILGGDHYFRFNHP 108

forkhead associated (FHA) domain found in the kinesin-like protein KIF16 family; The KIF16 family includes StARD9/KIF16A and KIF16B. StARD9, also called START domain-containing protein 9, or kinesin-like protein KIF16A, is a microtubule-dependent motor protein required for spindle pole assembly during mitosis. It is required to stabilize the pericentriolar material (PCM). KIF16B, also called sorting nexin-23, is a plus end-directed microtubule-dependent motor protein involved in endosome transport and receptor recycling and degradation. It regulates the plus end motility of early endosomes and the balance between recycling and degradation of receptors such as EGF receptor (EGFR) and FGF receptor (FGFR). It regulates the Golgi to endosome transport of FGFR-containing vesicles during early development, a key process for developing basement membrane and epiblast and primitive endoderm lineages during early postimplantation development. The FHA domain is a small phosphopeptide recognition module, but this group may lack the conserved residues that are required for binding phosphothreonine.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907080148   7 SSLQKKTPHLVNLNEDPLMSECLLYHIKDGVTRVGQVDV----DIKLTGQFIREQHCLFRSIpqpDGevMVTLEPCEGAE 82
Cdd:cd22708     2 VVLDSELPHLIGIDDDLLSTGVVLYHLKEGKTRIGREDApqeqDIVLDGEDIEAEHCIIENV---GG--VVTLHPLPGAL 76

                          90       100       110
                  ....*....|....*....|....*....|...
gi 1907080148  83 TYVNGKLVTEPLVLKSGNRIVMGKNHVFRFNHP 115
Cdd:cd22708    77 CAVNGQVITQPTRLTQGDVILLGKTNMFRFNHP 109

forkhead associated (FHA) domain found in the kinesin-like protein KIF13 family; The KIF13 family includes KIF13A and KIF13B. KIF13A, also called kinesin-like protein RBKIN, is a plus end-directed microtubule-dependent motor protein involved in intracellular transport and in regulating various processes such as mannose-6-phosphate receptor (M6PR) transport to the plasma membrane, endosomal sorting during melanosome biogenesis, and cytokinesis. It mediates the transport of M6PR-containing vesicles from trans-Golgi network to the plasma membrane via direct interaction with the AP-1 complex. During melanosome maturation, KIF13A is required for delivering melanogenic enzymes from recycling endosomes to nascent melanosomes by creating peripheral recycling endosomal subdomains in melanocytes. It is also required for the abscission step in cytokinesis: it mediates translocation of ZFYVE26, and possibly TTC19, to the midbody during cytokinesis. KIF13B, also called kinesin-like protein GAKIN, is a novel kinesin-like protein that associates with the human homolog of the Drosophila discs large tumor suppressor in T lymphocytes. It is involved in reorganization of the cortical cytoskeleton. It regulates axon formation by promoting the formation of extra axons. KIF13B may be functionally important for the intracellular trafficking of membrane-associated guanylate kinase homologs (MAGUKs) and associated protein complexes. The FHA domain is a small phosphopeptide recognition module, but this group may lack the conserved residues that are required for binding phosphothreonine.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907080148  16 LVNLNEDPLMSECLLYHIKDgVTRVGQVDV----DIKLTGQFIREQHCLfrsIPQPDGEVMVTlePCEGAETYVNGKLVT 91
Cdd:cd22706     4 LVNLNADPSLNELLVYYLKE-HTLIGRSDAptqqDIQLSGLGIQPEHCI---ITIENEDVYLT--PLEGARTCVNGSIVT 77

                          90       100
                  ....*....|....*....|....
gi 1907080148  92 EPLVLKSGNRIVMGKNHVFRFNHP 115
Cdd:cd22706    78 EKTQLRHGDRILWGNNHFFRLNCP 101

forkhead associated (FHA) domain found in kinesin-like protein KIF13A; KIF13A, also called kinesin-like protein RBKIN, is a plus end-directed microtubule-dependent motor protein involved in intracellular transport and in regulating various processes such as mannose-6-phosphate receptor (M6PR) transport to the plasma membrane, endosomal sorting during melanosome biogenesis, and cytokinesis. It mediates the transport of M6PR-containing vesicles from trans-Golgi network to the plasma membrane via direct interaction with the AP-1 complex. During melanosome maturation, KIF13A is required for delivering melanogenic enzymes from recycling endosomes to nascent melanosomes by creating peripheral recycling endosomal subdomains in melanocytes. It is also required for the abscission step in cytokinesis: it mediates translocation of ZFYVE26, and possibly TTC19, to the midbody during cytokinesis. The FHA domain is a small phosphopeptide recognition module, but this group may lack the conserved residues that are required for binding phosphothreonine.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907080148  16 LVNLNEDPLMSECLLYHIKDGvTRVG-QVDVDIKLTGQFIREQHCLFRSipQPDGEVmvTLEPCEGAETYVNGKLVTEPL 94
Cdd:cd22729     4 LVNLNADPALNELLVYYLKDH-TRVGaDTSQDIQLFGIGIQPEHCVIDI--AADGDV--TLTPKENARTCVNGTLVCSVT 78

                          90       100
                  ....*....|....*....|....*...
gi 1907080148  95 VLKSGNRIVMGKNHVFRFNHPEQARLER 122
Cdd:cd22729    79 QLWHGDRILWGNNHFFRINLPKRKRRDW 106

forkhead associated (FHA) domain found in pleckstrin homology-like domain family B member 1 (PHLDB1) and similar proteins; PHLDB1, also called protein LL5-alpha (LL5A), acts as an insulin-responsive protein that enhances Akt activation. PHLDB1 contains a pleckstrin homology domain, which binds phosphatidylinositol PI(3,4)P(2), PI(3,5)P(2), and PI(3,4,5)P(3), as well as a Forkhead-associated (FHA) domain and coiled coil regions. The FHA domain is a small phosphopeptide recognition module, but this group may lack the conserved residues that are required for binding phosphothreonine.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907080148  10 QKKTPHLVNLNEDPLMSECLLYHIKDGVTRVGQVDVD-IKLTGQFIREQHCLFRSIpqpDGEVmvTLEPCEGAETyVNGK 88
Cdd:cd22713    13 QTEKPHLVSLGSGRLSTAVTLLPLPEGKTTIGTAASDiISLQGPGVEPEHCYIENI---NGTV--TLYPCGNLCS-VDGL 86

                          90       100       110
                  ....*....|....*....|....*....|
gi 1907080148  89 LVTEPLVLKSGNRIVMGKNHVFRFNHPEQA 118
Cdd:cd22713    87 PITEPTRLTQGCMICLGRSNYFRFNHPAEA 116

Kinesin-associated;

                          10        20
                  ....*....|....*....|....*..
gi 1907080148  11 KKTPHLVNLNEDPLMSECLLYHIKDGV 37
Cdd:pfam16183 151 KKTPHLVNLNEDPLMSECLLYYIKDGI 177

forkhead associated (FHA) domain superfamily; Forkhead-associated (FHA) domains are small phosphopeptide recognition modules mostly found in eubacteria and eukaryotes. It is about 95-120 residues long that fold into an 11-stranded beta-sandwich. FHA domains can mediate the recognition of phosphorylated and non-phosphorylated substrates, as well as protein oligomerization. They specifically recognize threonine phosphorylation (pThr) accompanying activation of protein serine/threonine kinases. FHA domains show diverse ligand specificity. They may recognize the pTXXD motif, the pTXXI/L motif, and TQ clusters (singly and multiply phosphorylated). In eukaryotes, FHA superfamily members include forkhead-type transcription factors, as well as other signaling proteins, such as many regulatory proteins, kinases, phosphatases, motor proteins called kinesins, and metabolic enzymes. Many of them localize to the nucleus, where they participate in establishing or maintaining cell cycle checkpoints, DNA repair, or transcriptional regulation. FHA domains play important roles in human diseases, particularly in relation to DNA damage responses and cancers. In bacteria, FHA domain-containing proteins may participate in injection of viral proteins into host cells, transmembrane transporters, and cell division. FHA domain-containing proteins rarely include more than one copy of the domain. The only exception in eukaryotes is the checkpoint kinase Rad53 from Saccharomyces cerevisiae, which harbors two FHA domains (FHA1 and FHA2) flanking a central kinase domain. The two FHA domains recognize different phosphorylated targets and function independently from one another. In contrast, Mycobacterium tuberculosis ABC transporter Rv1747 contains two FHA domains but only one of them is essential for protein function.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907080148  15 HLVNLNEDPLMSEcllYHIKDGVTRVG-QVDVDIKLTGQFIREQHCLFRSIpqpDGEVMVT-LEPCEGaeTYVNGKLVTE 92
Cdd:cd00060     1 RLIVLDGDGGGRE---FPLTKGVVTIGrSPDCDIVLDDPSVSRRHARIEVD---GGGVYLEdLGSTNG--TFVNGKRITP 72

                          90       100
                  ....*....|....*....|
gi 1907080148  93 PLVLKSGNRIVMGkNHVFRF 112
Cdd:cd00060    73 PVPLQDGDVIRLG-DTTFRF 91

Forkhead associated (FHA) domain, binds pSer, pThr, pTyr [Signal transduction mechanisms];

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907080148  31 YHIKDGVTRVG-QVDVDIKLTGQFIREQHCLFRsiPQPDGevmVTLEPCEGA-ETYVNGKLVTEPLVLKSGNRIVMGKnH 108
Cdd:COG1716    16 FPLDGGPLTIGrAPDNDIVLDDPTVSRRHARIR--RDGGG---WVLEDLGSTnGTFVNGQRVTEPAPLRDGDVIRLGK-T 89

                  ....
gi 1907080148 109 VFRF 112
Cdd:COG1716    90 ELRF 93

forkhead associated (FHA) domain found in proliferation marker protein Ki-67 and similar proteins; Ki-67, also called antigen identified by monoclonal antibody Ki-67, antigen KI-67, or antigen Ki67, acts as a biological surfactant to disperse mitotic chromosomes. It is required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Ki-67 binds DNA with a preference for supercoiled DNA and AT-rich DNA. It may also play a role in chromatin organization. Ki-67 contains an FHA domain at its N-terminus. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1907080148  46 DIKLTGQFIREQHCLFRsipqPDGEVMVTLEP-CEGAETYVNGKLVTEPLVLKSGNRIVMGkNHVFRF 112
Cdd:cd22673    32 DIRIQLPGVSREHCRIE----VDENGKAYLENlSTTNPTLVNGKAIEKSAELKDGDVITIG-GRSFRF 94