NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|1903075921|dbj|GGY34420|]
View 

hypothetical protein GCM10010384_47090 [Streptomyces djakartensis]

Protein Classification

ATP-binding SpoIIE family protein phosphatase( domain architecture ID 13014758)

SpoIIE family serine/threonine-protein phosphatase with a histidine kinase-like ATPase domain, catalyzes the dephosphorylation of phosphoserine and phosphothreonine residues of specific protein substrates

CATH:  3.60.40.10
EC:  3.1.3.16
Gene Ontology:  GO:0004722|GO:0006470|GO:0046872|GO:0005524
PubMed:  8415923|9192069|8819174|10637609|10339418
SCOP:  3000909

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
HATPase_RsbT-like cd16934
Histidine kinase-like ATPase domain of the anti sigma-B factor Bacillus subtilis serine ...
 32-137 1.63e-27

Histidine kinase-like ATPase domain of the anti sigma-B factor Bacillus subtilis serine/threonine-protein kinase RsbT, and related domains; This family includes the histidine kinase-like ATPase (HATPase) domain of Bacillus subtilis serine/threonine-protein kinase RsbT, a component of the stressosome signaling complex of Bacillus subtilis. The stressosome is formed from multiple copies of three proteins, a sensor protein RsbR, a scaffold protein RsbS, and RsbT, and responds to environmental changes by initiating a protein partner switching cascade. Stress perception increases the phosphorylation of RsbR and RsbS, by RsbT. Subsequent dissociation of RsbT from the stressosome activates the sigma-B cascade, leading to the release of the alternative sigma factor, sigma-B.


:

Pssm-ID: 340411 [Multi-domain]  Cd Length: 117  Bit Score: 106.30  E-value: 1.63e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1903075921  32 DERRTAAAALVATELATNLLKHAEGGQVLIDVVDPPVLRpggerprVVQIVAFDHGPGVPDVPAALRDGFSTTGSLGAGL 111
Cdd:cd16934    19 SEVRQAEIATAVTELARNLLKHAGGGQVLLEVVAEGGRV-------ALEILAVDQGPGIADVDEALRDGFSTGGGLGLGL 91

                          90       100
                  ....*....|....*....|....*.
gi 1903075921 112 GTCLRLADDFHLHSTPGRGTVAAARV 137
Cdd:cd16934    92 GGVRRLADEFDLHSAPGRGTVVVARK 117
SpoIIE super family cl47965
Stage II sporulation protein E (SpoIIE); This family contains a number of bacterial stage II ...
 311-435 2.66e-09

Stage II sporulation protein E (SpoIIE); This family contains a number of bacterial stage II sporulation E proteins (EC:3.1.3.16). These are required for formation of a normal polar septum during sporulation. The N-terminal region is hydrophobic and is expected to contain up to 12 membrane-spanning segments.


The actual alignment was detected with superfamily member pfam07228:

Pssm-ID: 462119 [Multi-domain]  Cd Length: 192  Bit Score: 56.89  E-value: 2.66e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1903075921 311 SGDRVTLMLADGLGHGPEAARASSAAVAALHRWAH--LAPAESLRRLHEAL----RGTRGAAVALAQLDLRSWRLRFAGI 384
Cdd:pfam07228   1 PDGRLALVIGDVMGHGLPAALLMGLLRTALRALAAegLDPAEVLKRLNRLLqrnlEEDMFATAVLAVYDPETGTLEYANA 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1903075921 385 G-NVGARLRTDGTWRPLLSRPG-IVGVHRPATLREEEADWAADRLLILHTDGL 435
Cdd:pfam07228  81 GhPPPLLLRPDGGVVELLESPGlPLGILPDAPYEVVELELEPGDTLLLYTDGL 133
 
Name Accession Description Interval E-value
HATPase_RsbT-like cd16934
Histidine kinase-like ATPase domain of the anti sigma-B factor Bacillus subtilis serine ...
 32-137 1.63e-27

Histidine kinase-like ATPase domain of the anti sigma-B factor Bacillus subtilis serine/threonine-protein kinase RsbT, and related domains; This family includes the histidine kinase-like ATPase (HATPase) domain of Bacillus subtilis serine/threonine-protein kinase RsbT, a component of the stressosome signaling complex of Bacillus subtilis. The stressosome is formed from multiple copies of three proteins, a sensor protein RsbR, a scaffold protein RsbS, and RsbT, and responds to environmental changes by initiating a protein partner switching cascade. Stress perception increases the phosphorylation of RsbR and RsbS, by RsbT. Subsequent dissociation of RsbT from the stressosome activates the sigma-B cascade, leading to the release of the alternative sigma factor, sigma-B.


Pssm-ID: 340411 [Multi-domain]  Cd Length: 117  Bit Score: 106.30  E-value: 1.63e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1903075921  32 DERRTAAAALVATELATNLLKHAEGGQVLIDVVDPPVLRpggerprVVQIVAFDHGPGVPDVPAALRDGFSTTGSLGAGL 111
Cdd:cd16934    19 SEVRQAEIATAVTELARNLLKHAGGGQVLLEVVAEGGRV-------ALEILAVDQGPGIADVDEALRDGFSTGGGLGLGL 91

                          90       100
                  ....*....|....*....|....*.
gi 1903075921 112 GTCLRLADDFHLHSTPGRGTVAAARV 137
Cdd:cd16934    92 GGVRRLADEFDLHSAPGRGTVVVARK 117
RsbW COG2172
Anti-sigma regulatory factor (Ser/Thr protein kinase) [Signal transduction mechanisms];
32-132 3.57e-11

Anti-sigma regulatory factor (Ser/Thr protein kinase) [Signal transduction mechanisms];


Pssm-ID: 441775 [Multi-domain]  Cd Length: 127  Bit Score: 60.70  E-value: 3.57e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1903075921  32 DERRTAAAALVATELATNLLKHA----EGGQVLIDVVDppvlrpggeRPRVVQIVAFDHGPGVPdvPAALRDGFSTTGSL 107
Cdd:COG2172    28 DEDDADDLVLAVSEAVTNAVRHAyggdPDGPVEVELEL---------DPDGLEIEVRDEGPGFD--PEDLPDPYSTLAEG 96

                          90       100
                  ....*....|....*....|....*
gi 1903075921 108 GAGLGTCLRLADDFHLHSTPGRGTV 132
Cdd:COG2172    97 GRGLFLIRRLMDEVEYESDPGGTTV 121
SpoIIE pfam07228
Stage II sporulation protein E (SpoIIE); This family contains a number of bacterial stage II ...
 311-435 2.66e-09

Stage II sporulation protein E (SpoIIE); This family contains a number of bacterial stage II sporulation E proteins (EC:3.1.3.16). These are required for formation of a normal polar septum during sporulation. The N-terminal region is hydrophobic and is expected to contain up to 12 membrane-spanning segments.


Pssm-ID: 462119 [Multi-domain]  Cd Length: 192  Bit Score: 56.89  E-value: 2.66e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1903075921 311 SGDRVTLMLADGLGHGPEAARASSAAVAALHRWAH--LAPAESLRRLHEAL----RGTRGAAVALAQLDLRSWRLRFAGI 384
Cdd:pfam07228   1 PDGRLALVIGDVMGHGLPAALLMGLLRTALRALAAegLDPAEVLKRLNRLLqrnlEEDMFATAVLAVYDPETGTLEYANA 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1903075921 385 G-NVGARLRTDGTWRPLLSRPG-IVGVHRPATLREEEADWAADRLLILHTDGL 435
Cdd:pfam07228  81 GhPPPLLLRPDGGVVELLESPGlPLGILPDAPYEVVELELEPGDTLLLYTDGL 133
RsbU COG2208
Phosphoserine phosphatase RsbU, regulator of sigma subunit [Signal transduction mechanisms, ...
 277-435 5.25e-09

Phosphoserine phosphatase RsbU, regulator of sigma subunit [Signal transduction mechanisms, Transcription];


Pssm-ID: 441810 [Multi-domain]  Cd Length: 435  Bit Score: 58.15  E-value: 5.25e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1903075921 277 LRGRSVPVAGVRAGGVNIPyaGAEYSGDAWTWVR-SGDRVTLMLADGLGHGPEAARASSAAVAALHRWAH--LAPAESLR 353
Cdd:COG2208   211 LPPRLPEVPGLDIAARYRP--ADEVGGDFYDVFPlDDGRLAVVIGDVSGHGVPAALLMAMLRSALRALARegLDPAEVLE 288

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1903075921 354 RLHEALRGT----RGAAVALAQLDLRSWRLRFAGIGNVGA-RLRTDGTWRPLLSRPGIVGVHRPATLREEEADWAADRLL 428
Cdd:COG2208   289 RLNRALYEDlgggRFVTAFLGVLDPETGRLTYANAGHPPPlLLRADGEVEELDGGGLPLGLLPDAEYEEHEIPLEPGDRL 368


                  ....*..
gi 1903075921 429 ILHTDGL 435
Cdd:COG2208   369 LLYTDGL 375
PP2C_SIG smart00331
Sigma factor PP2C-like phosphatases;
294-435 9.65e-06

Sigma factor PP2C-like phosphatases;


Pssm-ID: 214624 [Multi-domain]  Cd Length: 193  Bit Score: 46.19  E-value: 9.65e-06
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1903075921  294 IPYAGAEYSGDAWTWVRSGD-RVTLMLADGLGHGPEAARASSAAVAALHRWAHL--APAESLRRLHEALR----GTRGAA 366
Cdd:smart00331   9 YYEDATQVGGDFYDVVKLPEgRLLIAIADVMGKGLAAALAMSMARSALRTLLSEgiSLSQILERLNRAIYengeDGMFAT 88

                           90       100       110       120       130       140       150
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1903075921  367 VALAQLDLRSWRLRFAGIGNVGARLRT--DGTWRPLLSRPGIVGVHRPATLREEEADWAADRLLILHTDGL 435
Cdd:smart00331  89 LFLALYDFAGGTLSYANAGHSPPYLLRadGGLVEDLDDLGAPLGLEPDVEVDVRELTLEPGDLLLLYTDGL 159
 
Name Accession Description Interval E-value
HATPase_RsbT-like cd16934
Histidine kinase-like ATPase domain of the anti sigma-B factor Bacillus subtilis serine ...
 32-137 1.63e-27

Histidine kinase-like ATPase domain of the anti sigma-B factor Bacillus subtilis serine/threonine-protein kinase RsbT, and related domains; This family includes the histidine kinase-like ATPase (HATPase) domain of Bacillus subtilis serine/threonine-protein kinase RsbT, a component of the stressosome signaling complex of Bacillus subtilis. The stressosome is formed from multiple copies of three proteins, a sensor protein RsbR, a scaffold protein RsbS, and RsbT, and responds to environmental changes by initiating a protein partner switching cascade. Stress perception increases the phosphorylation of RsbR and RsbS, by RsbT. Subsequent dissociation of RsbT from the stressosome activates the sigma-B cascade, leading to the release of the alternative sigma factor, sigma-B.


Pssm-ID: 340411 [Multi-domain]  Cd Length: 117  Bit Score: 106.30  E-value: 1.63e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1903075921  32 DERRTAAAALVATELATNLLKHAEGGQVLIDVVDPPVLRpggerprVVQIVAFDHGPGVPDVPAALRDGFSTTGSLGAGL 111
Cdd:cd16934    19 SEVRQAEIATAVTELARNLLKHAGGGQVLLEVVAEGGRV-------ALEILAVDQGPGIADVDEALRDGFSTGGGLGLGL 91

                          90       100
                  ....*....|....*....|....*.
gi 1903075921 112 GTCLRLADDFHLHSTPGRGTVAAARV 137
Cdd:cd16934    92 GGVRRLADEFDLHSAPGRGTVVVARK 117
RsbW COG2172
Anti-sigma regulatory factor (Ser/Thr protein kinase) [Signal transduction mechanisms];
32-132 3.57e-11

Anti-sigma regulatory factor (Ser/Thr protein kinase) [Signal transduction mechanisms];


Pssm-ID: 441775 [Multi-domain]  Cd Length: 127  Bit Score: 60.70  E-value: 3.57e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1903075921  32 DERRTAAAALVATELATNLLKHA----EGGQVLIDVVDppvlrpggeRPRVVQIVAFDHGPGVPdvPAALRDGFSTTGSL 107
Cdd:COG2172    28 DEDDADDLVLAVSEAVTNAVRHAyggdPDGPVEVELEL---------DPDGLEIEVRDEGPGFD--PEDLPDPYSTLAEG 96

                          90       100
                  ....*....|....*....|....*
gi 1903075921 108 GAGLGTCLRLADDFHLHSTPGRGTV 132
Cdd:COG2172    97 GRGLFLIRRLMDEVEYESDPGGTTV 121
SpoIIE pfam07228
Stage II sporulation protein E (SpoIIE); This family contains a number of bacterial stage II ...
 311-435 2.66e-09

Stage II sporulation protein E (SpoIIE); This family contains a number of bacterial stage II sporulation E proteins (EC:3.1.3.16). These are required for formation of a normal polar septum during sporulation. The N-terminal region is hydrophobic and is expected to contain up to 12 membrane-spanning segments.


Pssm-ID: 462119 [Multi-domain]  Cd Length: 192  Bit Score: 56.89  E-value: 2.66e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1903075921 311 SGDRVTLMLADGLGHGPEAARASSAAVAALHRWAH--LAPAESLRRLHEAL----RGTRGAAVALAQLDLRSWRLRFAGI 384
Cdd:pfam07228   1 PDGRLALVIGDVMGHGLPAALLMGLLRTALRALAAegLDPAEVLKRLNRLLqrnlEEDMFATAVLAVYDPETGTLEYANA 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1903075921 385 G-NVGARLRTDGTWRPLLSRPG-IVGVHRPATLREEEADWAADRLLILHTDGL 435
Cdd:pfam07228  81 GhPPPLLLRPDGGVVELLESPGlPLGILPDAPYEVVELELEPGDTLLLYTDGL 133
RsbU COG2208
Phosphoserine phosphatase RsbU, regulator of sigma subunit [Signal transduction mechanisms, ...
 277-435 5.25e-09

Phosphoserine phosphatase RsbU, regulator of sigma subunit [Signal transduction mechanisms, Transcription];


Pssm-ID: 441810 [Multi-domain]  Cd Length: 435  Bit Score: 58.15  E-value: 5.25e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1903075921 277 LRGRSVPVAGVRAGGVNIPyaGAEYSGDAWTWVR-SGDRVTLMLADGLGHGPEAARASSAAVAALHRWAH--LAPAESLR 353
Cdd:COG2208   211 LPPRLPEVPGLDIAARYRP--ADEVGGDFYDVFPlDDGRLAVVIGDVSGHGVPAALLMAMLRSALRALARegLDPAEVLE 288

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1903075921 354 RLHEALRGT----RGAAVALAQLDLRSWRLRFAGIGNVGA-RLRTDGTWRPLLSRPGIVGVHRPATLREEEADWAADRLL 428
Cdd:COG2208   289 RLNRALYEDlgggRFVTAFLGVLDPETGRLTYANAGHPPPlLLRADGEVEELDGGGLPLGLLPDAEYEEHEIPLEPGDRL 368


                  ....*..
gi 1903075921 429 ILHTDGL 435
Cdd:COG2208   369 LLYTDGL 375
PP2C_SIG smart00331
Sigma factor PP2C-like phosphatases;
294-435 9.65e-06

Sigma factor PP2C-like phosphatases;


Pssm-ID: 214624 [Multi-domain]  Cd Length: 193  Bit Score: 46.19  E-value: 9.65e-06
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1903075921  294 IPYAGAEYSGDAWTWVRSGD-RVTLMLADGLGHGPEAARASSAAVAALHRWAHL--APAESLRRLHEALR----GTRGAA 366
Cdd:smart00331   9 YYEDATQVGGDFYDVVKLPEgRLLIAIADVMGKGLAAALAMSMARSALRTLLSEgiSLSQILERLNRAIYengeDGMFAT 88

                           90       100       110       120       130       140       150
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1903075921  367 VALAQLDLRSWRLRFAGIGNVGARLRT--DGTWRPLLSRPGIVGVHRPATLREEEADWAADRLLILHTDGL 435
Cdd:smart00331  89 LFLALYDFAGGTLSYANAGHSPPYLLRadGGLVEDLDDLGAPLGLEPDVEVDVRELTLEPGDLLLLYTDGL 159
HATPase_TmoS-FixL-DctS-like cd16920
Histidine kinase-like ATPase domain of two-component sensor histidine kinases similar to ...
 76-132 5.63e-04

Histidine kinase-like ATPase domain of two-component sensor histidine kinases similar to Rhizobium meliloti FixL, and Rhodobacter capsulatus DctS; includes hybrid sensor histidine kinase similar to Pseudomonas mendocina TmoS; This family includes the histidine kinase-like ATPase (HATPase) domains of various histidine kinases (HKs) of two-component signal transduction systems (TCSs), such as Pseudomonas mendocina TmoS HK of the TmoS-TmoT TCS, which controls the expression of the toluene-4-monooxygenase pathway, Rhizobium meliloti FixL HK of the FixL-FixJ TCS, which regulates the expression of the genes related to nitrogen fixation in the root nodule in response to O(2) levels, and Rhodobacter capsulatus DctS of the DctS-DctR TCS, which controls synthesis of the high-affinity C4-dicarboxylate transport system. Proteins having this HATPase domain also contain a histidine kinase dimerization and phosphoacceptor domain (HisKA) and PAS sensor domain(s); many are hybrid sensor histidine kinases as they also contain a REC signal receiver domain.


Pssm-ID: 340397 [Multi-domain]  Cd Length: 104  Bit Score: 39.30  E-value: 5.63e-04
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1903075921  76 PRVVQIVAFDHGPGVPD-VPAALRDGFSTTGS--LGAGLGTCLRLADDFH----LHSTPGRGTV 132
Cdd:cd16920    35 DRAVTISVKDTGPGIAEeVAGQLFDPFYTTKSegLGMGLSICRSIIEAHGgrlsVESPAGGGAT 98
HATPase_RsbW-like cd16936
Histidine kinase-like ATPase domain of RsbW, an anti sigma-B factor and serine-protein kinase ...
 39-132 1.99e-03

Histidine kinase-like ATPase domain of RsbW, an anti sigma-B factor and serine-protein kinase involved in regulating sigma-B during stress in Bacilli, and related domains; This family includes histidine kinase-like ATPase (HATPase) domain of RsbW, an anti sigma-B factor as well as a serine-protein kinase involved in regulating sigma-B during stress in Bacilli. The alternative sigma factor sigma-B is an important regulator of the general stress response of Bacillus cereus and B. subtilis. RsbW is an anti-sigma factor while RsbV is an anti-sigma factor antagonist (anti-anti-sigma factor). RsbW can also act as a kinase on RsbV. In a partner-switching mechanism, RsbW, RsbV, and sigma-B participate as follows: in non-stressed cells, sigma-B is present in an inactive form complexed with RsbW; in this form, sigma-B is unable to bind to RNA polymerase. Under stress, RsbV binds to RsbW, forming an RsbV-RsbW complex, and sigma-B is released to bind to RNA polymerase. RsbW may then act as a kinase on RsbV, phosphorylating a serine residue; RsbW is then released to bind to sigma-B, hence blocking its ability to bind RNA polymerase. A phosphatase then dephosphorylates RsbV so that it can again form a complex with RsbW, leading to the release of sigma-B.


Pssm-ID: 340413 [Multi-domain]  Cd Length: 91  Bit Score: 37.25  E-value: 1.99e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1903075921  39 AALVATELATNLLKHA--EGGQVLIDVvdppVLRPGGERprvVQIVAFDHGPGVPDVPAALRDGFSTTGslgaGLGTCL- 115
Cdd:cd16936     1 VELAVSEAVTNAVRHAyrHDGPGPVRL----ELDLDPDR---LRVEVTDSGPGFDPLRPADPDAGLREG----GRGLALi 69

                          90
                  ....*....|....*...
gi 1903075921 116 -RLADDFHLHSTPGRGTV 132
Cdd:cd16936    70 rALMDEVGYRRTPGGKTV 87
NtrB COG3852
Signal transduction histidine kinase NtrB, nitrogen specific [Signal transduction mechanisms];
49-132 7.09e-03

Signal transduction histidine kinase NtrB, nitrogen specific [Signal transduction mechanisms];


Pssm-ID: 443061 [Multi-domain]  Cd Length: 361  Bit Score: 38.67  E-value: 7.09e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1903075921  49 NLLKHA-----EGGQVLIDV-VDPPVLRPGGERPRVVQI-VAfDHGPGVPDvpaALR----DGFSTTGSLGAGLG--TCL 115
Cdd:COG3852   251 NLVRNAaeampEGGTITIRTrVERQVTLGGLRPRLYVRIeVI-DNGPGIPE---EILdrifEPFFTTKEKGTGLGlaIVQ 326

                          90       100
                  ....*....|....*....|..
gi 1903075921 116 RLADDfH-----LHSTPGRGTV 132
Cdd:COG3852   327 KIVEQ-HggtieVESEPGKGTT 347
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH