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Conserved domains on  [gi|1859657950|ref|YP_009846194|]
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tail fiber protein [Aeromonas phage 2L372D]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Tail_spike_N super family cl39985
Tail spike TSP1/Gp66 receptor binding N-terminal domain; Bacteriophages recognize and bind to ...
 128-190 3.99e-12

Tail spike TSP1/Gp66 receptor binding N-terminal domain; Bacteriophages recognize and bind to their hosts with the help of receptor-binding proteins (RBPs) that emanate from the phage particle in the form of fibers or tailspikes. RBPs of podovirus G7C tailspikes gp63.1 and gp66 are essential for infection of its natural host bacterium E. coli 4s. Gp63.1 and gp66 form a stable complex, in which the N-terminal part of gp66 serves as an attachment site for gp63.1 and anchors the gp63.1-gp66 complex to the G7C tail. The two N-terminal domains show 70% sequence identity to the N-terminal region of the CBA120 phage tailspike 1 (orf210, TSP1). The N-terminal domain of TSP1 is the virion head binding domain that interfaces with the phage baseplate. The N-terminal domain can be further divided into two subdomains, each beginning with a alpha-helix followed by an anti-parallel beta-sandwich. Subdomain two folds similarly to the chitin binding domain of Chitinase from Bacillus circulans.


The actual alignment was detected with superfamily member pfam18668:

Pssm-ID: 408449  Cd Length: 70  Bit Score: 62.20  E-value: 3.99e-12
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1859657950 128 VGYSIVAG-SFEQGATIVDSKQALLFELEGKGYAWKGAVPKTIPAGSTPASSGGLGPTAWEDQS 190
Cdd:pfam18668   6 VEYVISGGkTFSEGATLNSPREEILYDDEKQMYRWTGAFPKVVPAASTPDSTGGVGDGAWIYVG 69
NosD super family cl34609
Nitrous oxide reductase accessory protein NosD, contains tandem CASH domains [Inorganic ion ...
 526-749 2.18e-04

Nitrous oxide reductase accessory protein NosD, contains tandem CASH domains [Inorganic ion transport and metabolism];


The actual alignment was detected with superfamily member COG3420:

Pssm-ID: 442646 [Multi-domain]  Cd Length: 343  Bit Score: 44.52  E-value: 2.18e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1859657950 526 GSVFTGNTGINCWRGI-AARSREDVIANNRLFGNRAYPTSPytggdtyGMALNEGYTVGATIRDNYISGFARNVFINNSs 604
Cdd:COG3420   108 NAVIENNRIENNLFGIyLEGSDNNVIRNNTISGNRDLRADR-------GNGIHLWNSPGNVIEGNTISGGRDGIYLEFS- 179

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1859657950 605 prgagfeyNRVTVLNNRMAYCR--------NHIEIDNATPTDKSI------DSGIIIQGNQFDSHEREAILIGtYVRTAI 670
Cdd:COG3420   180 --------DNNVIRNNTIRNLRygihymysNDNLVEGNTFRDNGAgialmySKGNTVRGNTILGNSGYGILLK-ESSDSV 250

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1859657950 671 IKDNIFLDAPSGSFYQiktegNCVGIQVVGNSFKDSAKTLWVQA------ITDPVLGTNSIETAAITSVNNI----TRGN 740
Cdd:COG3420   251 IEGNTISGNGKGIFIY-----NSNRNTIRGNLFAGNGIGIHLTAgsegnrIYGNNFIGNRTQVKYVGGRDNEwsadGRGN 325


                  ....*....
gi 1859657950 741 QFSDSFVYD 749
Cdd:COG3420   326 YWSDYTGFD 334
 
Name Accession Description Interval E-value
Tail_spike_N pfam18668
Tail spike TSP1/Gp66 receptor binding N-terminal domain; Bacteriophages recognize and bind to ...
 128-190 3.99e-12

Tail spike TSP1/Gp66 receptor binding N-terminal domain; Bacteriophages recognize and bind to their hosts with the help of receptor-binding proteins (RBPs) that emanate from the phage particle in the form of fibers or tailspikes. RBPs of podovirus G7C tailspikes gp63.1 and gp66 are essential for infection of its natural host bacterium E. coli 4s. Gp63.1 and gp66 form a stable complex, in which the N-terminal part of gp66 serves as an attachment site for gp63.1 and anchors the gp63.1-gp66 complex to the G7C tail. The two N-terminal domains show 70% sequence identity to the N-terminal region of the CBA120 phage tailspike 1 (orf210, TSP1). The N-terminal domain of TSP1 is the virion head binding domain that interfaces with the phage baseplate. The N-terminal domain can be further divided into two subdomains, each beginning with a alpha-helix followed by an anti-parallel beta-sandwich. Subdomain two folds similarly to the chitin binding domain of Chitinase from Bacillus circulans.


Pssm-ID: 408449  Cd Length: 70  Bit Score: 62.20  E-value: 3.99e-12
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1859657950 128 VGYSIVAG-SFEQGATIVDSKQALLFELEGKGYAWKGAVPKTIPAGSTPASSGGLGPTAWEDQS 190
Cdd:pfam18668   6 VEYVISGGkTFSEGATLNSPREEILYDDEKQMYRWTGAFPKVVPAASTPDSTGGVGDGAWIYVG 69
NosD COG3420
Nitrous oxide reductase accessory protein NosD, contains tandem CASH domains [Inorganic ion ...
 526-749 2.18e-04

Nitrous oxide reductase accessory protein NosD, contains tandem CASH domains [Inorganic ion transport and metabolism];


Pssm-ID: 442646 [Multi-domain]  Cd Length: 343  Bit Score: 44.52  E-value: 2.18e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1859657950 526 GSVFTGNTGINCWRGI-AARSREDVIANNRLFGNRAYPTSPytggdtyGMALNEGYTVGATIRDNYISGFARNVFINNSs 604
Cdd:COG3420   108 NAVIENNRIENNLFGIyLEGSDNNVIRNNTISGNRDLRADR-------GNGIHLWNSPGNVIEGNTISGGRDGIYLEFS- 179

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1859657950 605 prgagfeyNRVTVLNNRMAYCR--------NHIEIDNATPTDKSI------DSGIIIQGNQFDSHEREAILIGtYVRTAI 670
Cdd:COG3420   180 --------DNNVIRNNTIRNLRygihymysNDNLVEGNTFRDNGAgialmySKGNTVRGNTILGNSGYGILLK-ESSDSV 250

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1859657950 671 IKDNIFLDAPSGSFYQiktegNCVGIQVVGNSFKDSAKTLWVQA------ITDPVLGTNSIETAAITSVNNI----TRGN 740
Cdd:COG3420   251 IEGNTISGNGKGIFIY-----NSNRNTIRGNLFAGNGIGIHLTAgsegnrIYGNNFIGNRTQVKYVGGRDNEwsadGRGN 325


                  ....*....
gi 1859657950 741 QFSDSFVYD 749
Cdd:COG3420   326 YWSDYTGFD 334
 
Name Accession Description Interval E-value
Tail_spike_N pfam18668
Tail spike TSP1/Gp66 receptor binding N-terminal domain; Bacteriophages recognize and bind to ...
 128-190 3.99e-12

Tail spike TSP1/Gp66 receptor binding N-terminal domain; Bacteriophages recognize and bind to their hosts with the help of receptor-binding proteins (RBPs) that emanate from the phage particle in the form of fibers or tailspikes. RBPs of podovirus G7C tailspikes gp63.1 and gp66 are essential for infection of its natural host bacterium E. coli 4s. Gp63.1 and gp66 form a stable complex, in which the N-terminal part of gp66 serves as an attachment site for gp63.1 and anchors the gp63.1-gp66 complex to the G7C tail. The two N-terminal domains show 70% sequence identity to the N-terminal region of the CBA120 phage tailspike 1 (orf210, TSP1). The N-terminal domain of TSP1 is the virion head binding domain that interfaces with the phage baseplate. The N-terminal domain can be further divided into two subdomains, each beginning with a alpha-helix followed by an anti-parallel beta-sandwich. Subdomain two folds similarly to the chitin binding domain of Chitinase from Bacillus circulans.


Pssm-ID: 408449  Cd Length: 70  Bit Score: 62.20  E-value: 3.99e-12
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1859657950 128 VGYSIVAG-SFEQGATIVDSKQALLFELEGKGYAWKGAVPKTIPAGSTPASSGGLGPTAWEDQS 190
Cdd:pfam18668   6 VEYVISGGkTFSEGATLNSPREEILYDDEKQMYRWTGAFPKVVPAASTPDSTGGVGDGAWIYVG 69
NosD COG3420
Nitrous oxide reductase accessory protein NosD, contains tandem CASH domains [Inorganic ion ...
 526-749 2.18e-04

Nitrous oxide reductase accessory protein NosD, contains tandem CASH domains [Inorganic ion transport and metabolism];


Pssm-ID: 442646 [Multi-domain]  Cd Length: 343  Bit Score: 44.52  E-value: 2.18e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1859657950 526 GSVFTGNTGINCWRGI-AARSREDVIANNRLFGNRAYPTSPytggdtyGMALNEGYTVGATIRDNYISGFARNVFINNSs 604
Cdd:COG3420   108 NAVIENNRIENNLFGIyLEGSDNNVIRNNTISGNRDLRADR-------GNGIHLWNSPGNVIEGNTISGGRDGIYLEFS- 179

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1859657950 605 prgagfeyNRVTVLNNRMAYCR--------NHIEIDNATPTDKSI------DSGIIIQGNQFDSHEREAILIGtYVRTAI 670
Cdd:COG3420   180 --------DNNVIRNNTIRNLRygihymysNDNLVEGNTFRDNGAgialmySKGNTVRGNTILGNSGYGILLK-ESSDSV 250

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1859657950 671 IKDNIFLDAPSGSFYQiktegNCVGIQVVGNSFKDSAKTLWVQA------ITDPVLGTNSIETAAITSVNNI----TRGN 740
Cdd:COG3420   251 IEGNTISGNGKGIFIY-----NSNRNTIRGNLFAGNGIGIHLTAgsegnrIYGNNFIGNRTQVKYVGGRDNEwsadGRGN 325


                  ....*....
gi 1859657950 741 QFSDSFVYD 749
Cdd:COG3420   326 YWSDYTGFD 334
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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