NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|1848022117|ref|WP_172674734|]
View 

nitroreductase family protein [Bifidobacterium pseudocatenulatum]

Protein Classification

nitroreductase family protein( domain architecture ID 104)

nitroreductase family protein may catalyze the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles, requiring NAD(P)H as an electron donor in an obligatory two-electron transfer and using FMN as cofactor

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
Nitro_FMN_reductase super family cl00514
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 6-231 5.44e-77

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


The actual alignment was detected with superfamily member cd02146:

Pssm-ID: 444950 [Multi-domain]  Cd Length: 229  Bit Score: 232.51  E-value: 5.44e-77
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117   6 TVDTLLERRSIRKFKPKPLSDDIVETLETVAQHAASSQFLNDWSAIRITDPAAKKRLAEI-GGQPYIATAPLLYVFVLDE 84
Cdd:cd02146     1 TIETILNHRSVRKFTDEPLTDETLETLIAAAQSASTSSNLQAYSVIVVTDPELREKLAELaGNQPYVAQAPVFLVFCADL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  85 HRNAAIAASKGVetasDEFTLNGSYRYTQAQNDAVLALHAMETAAYSLSLGCVILGSLLNDVPALIDLLNLPEYTYPVLG 164
Cdd:cd02146    81 YRHQKIAEEAGG----KDVGLDYLESFLVGVVDAALAAQNALVAAESLGLGIVYIGGIRNNPEEVIELLGLPEYVFPLFG 156

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1848022117 165 LAIGKPDQDPALKPRMPRPMQFFENEYPSNneTLLAGLQDFDEEVHRYYDLRNTERPVDPFSDQIAS 231
Cdd:cd02146   157 LTVGHPDPTPEVKPRLPLEAVVHEETYQDL--TDLEDLAAYDETMSEYYAERGSNKRTETWSQQIAD 221
 
Name Accession Description Interval E-value
NfsA-like cd02146
nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin ...
 6-231 5.44e-77

nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin reductase and oxygen-insensitive nitroreductase. These enzymes are homodimeric flavoproteins that contain one FMN per monomer as a cofactor. Flavin reductase catalyzes the reduction of flavin by using NADPH as an electron donor. Oxygen-insensitive nitroreductase, such as NfsA protein in Escherichia coli, catalyzes reduction of nitrocompounds using NADPH as electron donor.


Pssm-ID: 380322 [Multi-domain]  Cd Length: 229  Bit Score: 232.51  E-value: 5.44e-77
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117   6 TVDTLLERRSIRKFKPKPLSDDIVETLETVAQHAASSQFLNDWSAIRITDPAAKKRLAEI-GGQPYIATAPLLYVFVLDE 84
Cdd:cd02146     1 TIETILNHRSVRKFTDEPLTDETLETLIAAAQSASTSSNLQAYSVIVVTDPELREKLAELaGNQPYVAQAPVFLVFCADL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  85 HRNAAIAASKGVetasDEFTLNGSYRYTQAQNDAVLALHAMETAAYSLSLGCVILGSLLNDVPALIDLLNLPEYTYPVLG 164
Cdd:cd02146    81 YRHQKIAEEAGG----KDVGLDYLESFLVGVVDAALAAQNALVAAESLGLGIVYIGGIRNNPEEVIELLGLPEYVFPLFG 156

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1848022117 165 LAIGKPDQDPALKPRMPRPMQFFENEYPSNneTLLAGLQDFDEEVHRYYDLRNTERPVDPFSDQIAS 231
Cdd:cd02146   157 LTVGHPDPTPEVKPRLPLEAVVHEETYQDL--TDLEDLAAYDETMSEYYAERGSNKRTETWSQQIAD 221
PRK10765 PRK10765
oxygen-insensitive NADPH nitroreductase;
4-252 1.66e-56

oxygen-insensitive NADPH nitroreductase;


Pssm-ID: 182710  Cd Length: 240  Bit Score: 180.55  E-value: 1.66e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117   4 NATVDTLLERRSIRKFKPKPLSDDIVETLETVAQHAASSQFLNDWSAIRITDPAAKKRLAEI-GGQPYIATAPLLYVFVL 82
Cdd:PRK10765    2 TPTIELILSHRSIRHFTDEPISEAQREAIINAARAASSSSFLQCSSIIRITDKALREALVELtGGQKYVAQAAEFWVFCA 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  83 DEHRNAAIAASkgVETASDEFTLNGsyrytqAQNDAVLALHAMeTAAYSLSLGCVILGSLLNDVPALIDLLNLPEYTYPV 162
Cdd:PRK10765   82 DFNRHLQICPD--AQLGLAEQLLIG------AVDTAIMAQNAL-LAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPL 152

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117 163 LGLAIGKPDQDPALKPRMPRPMQFFENEYPSNNETLLAglqDFDEEVHRYYDLRNTERPVDPFSDQIASnavdsgVTSKA 242
Cdd:PRK10765  153 FGLCLGWPAQNPDLKPRLPASLLVHENQYQPLDKDLLA---QYDEQLAEYYLTRSSNNRQDTWSDHIRR------TLIKE 223

                         250
                  ....*....|....
gi 1848022117 243 VAPNAK----RQGF 252
Cdd:PRK10765  224 SRPFILdylhKQGW 237
NfnB COG0778
Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway ...
 8-181 1.56e-36

Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440541 [Multi-domain]  Cd Length: 163  Bit Score: 126.89  E-value: 1.56e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117   8 DTLLERRSIRKFKPKPLSDDIVETLETVAQHAASSQFLNDWSAIRITDPAAKKRLAEI---GGQPYIATAPLLYVFVLDE 84
Cdd:COG0778     3 ELLLTRRSVRKFTDKPVSDEELEELLEAARLAPSAGNLQPWRFVVVRDPELRERLAEAlaeANQEWVADAPVLIVVCADP 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  85 HRNAAIAaskgvetasdeftlngsyrYTQAQNDAVLALHAMETAAYSLSLGCVILGSLlnDVPALIDLLNLPEYTYPVLG 164
Cdd:COG0778    83 DRSEKVP-------------------ERYALLDAGIAAQNLLLAARALGLGTCWIGGF--DPEKVRELLGLPEGEEPVAL 141

                         170
                  ....*....|....*..
gi 1848022117 165 LAIGKPDQDPALKPRMP 181
Cdd:COG0778   142 LALGYPAEELNPRPRKP 158
Nitroreductase pfam00881
Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent ...
 10-169 2.47e-19

Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent and NAD(P)H-dependent enzymes able to metabolize nitrosubstituted compounds.


Pssm-ID: 425926 [Multi-domain]  Cd Length: 168  Bit Score: 82.05  E-value: 2.47e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  10 LLERRSIRKFKPKPLSDDIVETLETVAQHAASSQFLNDWSAIRITDPAAKKRLAE-IGGQPYIATAPLLYVFVLDEHRNA 88
Cdd:pfam00881   1 IRQRRSVRKFDPEPVPKEVLEEILEAARRAPSAGNLQPWRFYVVTDGELRYRLAEaALELLLVEPAAALLLLLRRDANLK 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  89 AIAASKG---------VETASDEFTLNGSYRYTQAQNDAVLALHAMETAAYSLSLGCVILGSLLNDvpALIDLLNLPEYT 159
Cdd:pfam00881  81 LLLQDFLrgapvliviTASLSTYLRKAAERAYREALLDAGAAAQNLLLAATSLGLGSCPIGGFDAA--AVRELLGLPDDE 158

                         170
                  ....*....|
gi 1848022117 160 YPVLGLAIGK 169
Cdd:pfam00881 159 RLVGLIAVGY 168
 
Name Accession Description Interval E-value
NfsA-like cd02146
nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin ...
 6-231 5.44e-77

nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin reductase and oxygen-insensitive nitroreductase. These enzymes are homodimeric flavoproteins that contain one FMN per monomer as a cofactor. Flavin reductase catalyzes the reduction of flavin by using NADPH as an electron donor. Oxygen-insensitive nitroreductase, such as NfsA protein in Escherichia coli, catalyzes reduction of nitrocompounds using NADPH as electron donor.


Pssm-ID: 380322 [Multi-domain]  Cd Length: 229  Bit Score: 232.51  E-value: 5.44e-77
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117   6 TVDTLLERRSIRKFKPKPLSDDIVETLETVAQHAASSQFLNDWSAIRITDPAAKKRLAEI-GGQPYIATAPLLYVFVLDE 84
Cdd:cd02146     1 TIETILNHRSVRKFTDEPLTDETLETLIAAAQSASTSSNLQAYSVIVVTDPELREKLAELaGNQPYVAQAPVFLVFCADL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  85 HRNAAIAASKGVetasDEFTLNGSYRYTQAQNDAVLALHAMETAAYSLSLGCVILGSLLNDVPALIDLLNLPEYTYPVLG 164
Cdd:cd02146    81 YRHQKIAEEAGG----KDVGLDYLESFLVGVVDAALAAQNALVAAESLGLGIVYIGGIRNNPEEVIELLGLPEYVFPLFG 156

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1848022117 165 LAIGKPDQDPALKPRMPRPMQFFENEYPSNneTLLAGLQDFDEEVHRYYDLRNTERPVDPFSDQIAS 231
Cdd:cd02146   157 LTVGHPDPTPEVKPRLPLEAVVHEETYQDL--TDLEDLAAYDETMSEYYAERGSNKRTETWSQQIAD 221
PRK10765 PRK10765
oxygen-insensitive NADPH nitroreductase;
4-252 1.66e-56

oxygen-insensitive NADPH nitroreductase;


Pssm-ID: 182710  Cd Length: 240  Bit Score: 180.55  E-value: 1.66e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117   4 NATVDTLLERRSIRKFKPKPLSDDIVETLETVAQHAASSQFLNDWSAIRITDPAAKKRLAEI-GGQPYIATAPLLYVFVL 82
Cdd:PRK10765    2 TPTIELILSHRSIRHFTDEPISEAQREAIINAARAASSSSFLQCSSIIRITDKALREALVELtGGQKYVAQAAEFWVFCA 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  83 DEHRNAAIAASkgVETASDEFTLNGsyrytqAQNDAVLALHAMeTAAYSLSLGCVILGSLLNDVPALIDLLNLPEYTYPV 162
Cdd:PRK10765   82 DFNRHLQICPD--AQLGLAEQLLIG------AVDTAIMAQNAL-LAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPL 152

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117 163 LGLAIGKPDQDPALKPRMPRPMQFFENEYPSNNETLLAglqDFDEEVHRYYDLRNTERPVDPFSDQIASnavdsgVTSKA 242
Cdd:PRK10765  153 FGLCLGWPAQNPDLKPRLPASLLVHENQYQPLDKDLLA---QYDEQLAEYYLTRSSNNRQDTWSDHIRR------TLIKE 223

                         250
                  ....*....|....
gi 1848022117 243 VAPNAK----RQGF 252
Cdd:PRK10765  224 SRPFILdylhKQGW 237
NfnB COG0778
Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway ...
 8-181 1.56e-36

Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440541 [Multi-domain]  Cd Length: 163  Bit Score: 126.89  E-value: 1.56e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117   8 DTLLERRSIRKFKPKPLSDDIVETLETVAQHAASSQFLNDWSAIRITDPAAKKRLAEI---GGQPYIATAPLLYVFVLDE 84
Cdd:COG0778     3 ELLLTRRSVRKFTDKPVSDEELEELLEAARLAPSAGNLQPWRFVVVRDPELRERLAEAlaeANQEWVADAPVLIVVCADP 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  85 HRNAAIAaskgvetasdeftlngsyrYTQAQNDAVLALHAMETAAYSLSLGCVILGSLlnDVPALIDLLNLPEYTYPVLG 164
Cdd:COG0778    83 DRSEKVP-------------------ERYALLDAGIAAQNLLLAARALGLGTCWIGGF--DPEKVRELLGLPEGEEPVAL 141

                         170
                  ....*....|....*..
gi 1848022117 165 LAIGKPDQDPALKPRMP 181
Cdd:COG0778   142 LALGYPAEELNPRPRKP 158
Nitro_FMN_reductase cd02062
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 10-168 6.90e-23

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380311 [Multi-domain]  Cd Length: 139  Bit Score: 90.82  E-value: 6.90e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  10 LLERRSIRKFKPKPLSDDIVETLETVAQHAASSQFLNDWSAIRITDPAAKKRLAEI--GGQPYIATAPLLYVFVLDEHRN 87
Cdd:cd02062     1 IKTRRSIRKFTDKPVPEEKLRKILEAARLAPSAGNLQPWRFIVVRDREKKEKLAKLaaPNQKFIAGAPVVIVVVADPDKS 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  88 AAIAAskgvetasdeftlngsyrytqaqNDAVLALHAMETAAYSLSLGCVILGSLLNDVPALIDLLNLPEYTYPVLGLAI 167
Cdd:cd02062    81 RPWAL-----------------------EDAGAAAQNLLLAAAALGLGSCWIGGFDFREDKVRELLGIPENLRPVALIAI 137


                  .
gi 1848022117 168 G 168
Cdd:cd02062   138 G 138
Nitroreductase pfam00881
Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent ...
 10-169 2.47e-19

Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent and NAD(P)H-dependent enzymes able to metabolize nitrosubstituted compounds.


Pssm-ID: 425926 [Multi-domain]  Cd Length: 168  Bit Score: 82.05  E-value: 2.47e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  10 LLERRSIRKFKPKPLSDDIVETLETVAQHAASSQFLNDWSAIRITDPAAKKRLAE-IGGQPYIATAPLLYVFVLDEHRNA 88
Cdd:pfam00881   1 IRQRRSVRKFDPEPVPKEVLEEILEAARRAPSAGNLQPWRFYVVTDGELRYRLAEaALELLLVEPAAALLLLLRRDANLK 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  89 AIAASKG---------VETASDEFTLNGSYRYTQAQNDAVLALHAMETAAYSLSLGCVILGSLLNDvpALIDLLNLPEYT 159
Cdd:pfam00881  81 LLLQDFLrgapvliviTASLSTYLRKAAERAYREALLDAGAAAQNLLLAATSLGLGSCPIGGFDAA--AVRELLGLPDDE 158

                         170
                  ....*....|
gi 1848022117 160 YPVLGLAIGK 169
Cdd:pfam00881 159 RLVGLIAVGY 168
nitroreductase cd02139
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 8-181 2.12e-18

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380316 [Multi-domain]  Cd Length: 165  Bit Score: 79.44  E-value: 2.12e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117   8 DTLLERRSIRKFKPKPLSDDIVETLETVAQHAASSQFLNDWSAIRITDPAAKKRLAE-IGGQPYIATAPLLYVFVLDEhr 86
Cdd:cd02139     3 EAIKKRRSIRKYKPTPVEEEKLLRILEAARLAPSAKNRQPWRFIVVKDKELKEKLAEaANGQKFIAEAPVVIVACADP-- 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  87 naaiaaskgvetaSDEFTLNGSYRYTQaqnDAVLALHAMETAAYSLSLGCVILGSLLNDvpALIDLLNLPEyTYPVLGL- 165
Cdd:cd02139    81 -------------SESGMGCGKPYYLV---DVAIAMEHLVLAATEEGLGTCWIGAFDED--KVKEILGIPE-EYRVVALt 141

                         170
                  ....*....|....*.
gi 1848022117 166 AIGKPDQDPALKPRMP 181
Cdd:cd02139   142 PLGYPAEEPPPRPRKP 157
PnbA_NfnB-like cd02136
nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as ...
 9-182 3.32e-17

nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as a cofactor and catalyze reduction of a variety of nitroaromatic compounds, including nitrofurans, nitrobenzens, nitrophenol, nitrobenzoate and quinones by using either NADH or NADPH as a source of reducing equivalents in an obligatory two-election transfer mechanism. The enzyme is typically a homodimer. Mycobacterium smegmatis nitroreductase NfnB plays a role in resistance to benzothiazinone.


Pssm-ID: 380313 [Multi-domain]  Cd Length: 152  Bit Score: 76.09  E-value: 3.32e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117   9 TLLERRSIRKFKPKPLSDDIVETLETVAQHAASSQFLNDWSAIRITDpAAKKRLAEIGgqpyiATAPLLYVFVLDehrna 88
Cdd:cd02136     1 AIKSRRSVRAFKDKPVPKETIEKILEAARRAPSGKNTQPWRVYVVTG-KARERLKKAF-----FGAPVALFLTMD----- 69

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  89 aiaasKGVETASDEftlngsyrytqaqnDAVLALHAMETAAYSLSLGCVILGSLLNDVPALIDLLNLPEYTYPVLGLAIG 168
Cdd:cd02136    70 -----KVLGPWSWF--------------DLGAFLQNLMLAAHALGLGTCPQGALAGYPDVVRKELGIPDDEELVCGIALG 130

                         170
                  ....*....|....*
gi 1848022117 169 KPDQD-PALKPRMPR 182
Cdd:cd02136   131 YPDPDaPVNQFRTPR 145
MhqN-like cd02137
nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily ...
 8-181 3.59e-16

nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily of the nitroreductase family containing uncharacterized proteins; includes nitroreductases MhqN, YodC, YdgI, DrgA. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380314 [Multi-domain]  Cd Length: 147  Bit Score: 73.04  E-value: 3.59e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117   8 DTLLERRSIRKFKP-KPLSDDIVETLETVAQHAASSQFLNDWSAIRITDPAAKKRLAEI-GGQPYIATAPLLYVFVLDeh 85
Cdd:cd02137     2 EVIKSRRSVRNFDPdHKIPKEELKEILELATLAPSSFNLQPWRFVVVRDPELKAKLAEAaYNQPQVTTASAVILVLGD-- 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  86 RNAAIAaskgvetasdeftlngsyrytqAQNdAVLALHAM--ETaayslslgCVILGSllnDVPALIDLLNLPEYTYPVL 163
Cdd:cd02137    80 LNAGLA----------------------AMN-LMLAAKAKgyDT--------CPMGGF---DKEKVAELLNLPDRYVPVL 125

                         170
                  ....*....|....*...
gi 1848022117 164 GLAIGKPDQDPALKPRMP 181
Cdd:cd02137   126 LIAIGKAADKAPRSGRLP 143
nitroreductase cd02151
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 8-178 3.96e-16

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers..


Pssm-ID: 380326 [Multi-domain]  Cd Length: 157  Bit Score: 73.34  E-value: 3.96e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117   8 DTLLERRSIRKFKPKPLSDDIVETLETVAQHAASSQFLNDWSAIRITDPAAKKRLAEI--GGQPYIATAPLLYVFVLDEh 85
Cdd:cd02151     1 ELLKKRRSIRKYTDEPIEEEKLEEILEAALLAPSSRNSRPVEFIVVDDKETLKKLSECkpHGSAFLKGAPAAIVVLADT- 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  86 rnaaiaaskgveTASDeftlngsyRYTQaqnDAVLALHAMETAAYSLSLGC----VILGSLLNDVPA---LIDLLNLPEy 158
Cdd:cd02151    80 ------------EKSD--------TWIE---DASIAATYIQLAAESLGLGScwiqIRNRETQDGKTAeeyVRELLGIPE- 135

                         170       180
                  ....*....|....*....|.
gi 1848022117 159 TYPVLG-LAIGKPDQDPALKP 178
Cdd:cd02151   136 NYRVLCiIALGYPDEEKPPHE 156
nitroreductase_FeS-like cd02143
nitroreductases with an N-terminal iron-sulfur cluster-binding domain; Members of this family ...
 13-170 3.26e-14

nitroreductases with an N-terminal iron-sulfur cluster-binding domain; Members of this family utilize FMN as a cofactor. This family may be involved in the reduction of flavin or nitroaromatic compounds via an obligatory two-electron transfer. Nitroreductase is homodimer. Each subunit contains one FMN molecule.


Pssm-ID: 380319 [Multi-domain]  Cd Length: 187  Bit Score: 68.65  E-value: 3.26e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  13 RRSIRKFKPKPLSDDIVETLETVAQHAAS---SQFLNdWSAirITDPAAKKRLAE---------IGGQPYIATAPLLYVF 80
Cdd:cd02143     5 RRSIRRYKDKPVPRETLEKLLDIARYAPTghnSQPVH-WLV--VDDPEKVRRLAElvidwmrelIKEDPELAGKLFLDGI 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  81 V------LDE-HRNA---AIA-ASKGVETASDeftlngsyrytqaqnDAVLALHAMETAAYSLSLGCVILGSL---LNDV 146
Cdd:cd02143    82 VaawekgIDViLRGAphlVVAhAPKDAPTPPV---------------DCAIALTYLELAAPSLGLGTCWAGFFtaaANNY 146

                         170       180
                  ....*....|....*....|....*
gi 1848022117 147 PALIDLLNLPEyTYPVLG-LAIGKP 170
Cdd:cd02143   147 PPLREALGLPE-GHKVGGaMMLGYP 170
nitroreductase cd02150
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 10-179 1.02e-11

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers.


Pssm-ID: 380325 [Multi-domain]  Cd Length: 156  Bit Score: 61.46  E-value: 1.02e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  10 LLERRSIRKFKPKPLSDDIVETLETVAQHAASSQFLNDWSAIRITDPAAKKRLAEIggQPY---IATAPLlyvfvldehr 86
Cdd:cd02150     1 ILTRRSIRKYTDKPVEEEDIEKLLRAAMAAPSAGNQQPWHFIVVTDREKLDKIAEA--HPYgkmLKEAPL---------- 68

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  87 naAIAaskgveTASDEfTLNGSYRYTQAqnDAVLALHAMETAAYSLSLGCVILGSLLND--VPALIDLLNLPEYTYPVLG 164
Cdd:cd02150    69 --AIV------VCGDP-SKEKAPGYWVQ--DCSAATENILLAAHALGLGAVWLGVYPFEerVKAIREILNIPENIIPFCV 137

                         170
                  ....*....|....*
gi 1848022117 165 LAIGKPDQDPALKPR 179
Cdd:cd02150   138 IALGYPAEEKEPKDR 152
BluB cd02145
5,6-dimethylbenzimidazole synthase; BluB catalyzes the O2-dependent conversion of FMNH2 to 5, ...
 10-171 2.77e-11

5,6-dimethylbenzimidazole synthase; BluB catalyzes the O2-dependent conversion of FMNH2 to 5,6-dimethylbenzimidazole (DMB), a component of vitamin B12; is is a subfamily of the nitroreductase family; nitroreductases typically reduce their substrates by using NAD(P)H as electron donor and often use FMN as a cofactor.


Pssm-ID: 380321  Cd Length: 196  Bit Score: 60.83  E-value: 2.77e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  10 LLERRSIRKFKPKPLSDDIVETLETVAQHAASSQFLNDWSAIRITDPAAKKRLAEIggqpyiatapllyvfVLDEHRNAA 89
Cdd:cd02145     4 IRWRRDVRHFRPDPVPEEVLERLLQAAHLAPSVGLMQPWRFVRVRSAATRKAVHEL---------------FQRANAEAA 68

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  90 IAASKGVETASDEFTLNG----------SYRYTQAQNDAVLALHAMETAAYS----------------LSLGCVilgSLL 143
Cdd:cd02145    69 EMYTGERAAQYRTLKLEGieeaplqlavFCDRARAGGHGLGRTTMPEMDLYSsvcavqnlwlaaraegLGVGWV---SIL 145

                         170       180
                  ....*....|....*....|....*...
gi 1848022117 144 NDVpALIDLLNLPEYTYPVLGLAIGKPD 171
Cdd:cd02145   146 DPD-EVKRLLGIPEHWEPVAYLCIGYPE 172
nitroreductase cd20608
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 13-168 9.96e-10

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380329 [Multi-domain]  Cd Length: 145  Bit Score: 55.42  E-value: 9.96e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  13 RRSIRKFKPKPLSDDIVETLETVAQHAASSQFLNDWSAIRITDPAAKKRLAEIGGQP--YIATAPLLYVFVLDEHRNAai 90
Cdd:cd20608     7 RRSVRRFSDKPVEEEKLEKILEAARLAPSWANKQCWRFIVVTDKETLSELAKKESPSngWLKDAPVIIVVCADPKDSG-- 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  91 aaskgvetasdefTLNGS--YRYtqaqnDAVLALHAMETAAYSLSLGCVILGSLlnDVPALIDLLNLPEYTYPVLGLAIG 168
Cdd:cd20608    85 -------------WLNGQnyYLV-----DAAIAMQNLMLAATDLGLGTCWIGAF--DEKKVKEILGIPENIRVVALTPLG 144
NfsB-like cd02149
nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This ...
 7-182 7.53e-09

nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This domain catalyzes the reduction of flavin, nitrocompound, quinones and azo compounds using NADH or NADPH as an electron donor. The enzyme is a homodimer, and each monomer binds a FMN as co-factor. This family includes FRase I in Vibrio fischeri, wihich reduces FMN into FMNH2 as part of the bioluminescent reaction. The family also includes oxygen-insensitive nitroreductases that use NADH or NADPH as an electron donor in the ping pong bi bi mechanism. This type of nitroreductase can be used in cancer chemotherapy to activate a range of prodrugs.


Pssm-ID: 380324 [Multi-domain]  Cd Length: 156  Bit Score: 53.41  E-value: 7.53e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117   7 VDTLLERRSIRKFKP-KPLSDDIVETLETVAQHAASSQFLNDWSAIRITDPAAKKRLAEI--GGQPYIATAPLLYVFVLD 83
Cdd:cd02149     3 LELLNFRYATKKFDPnKKISDEDLETILEALRLSPSSFGLEPWKFLVVENPELKAKLAPAawFNQPQIKDASHVVVFLAK 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  84 EHrnaaiaaskgvetasdeftlngsyrYTQAQndAVLALHAMETAAYSLSLG-CVILGSllnDVPALIDLLNLPEYTY-P 161
Cdd:cd02149    83 KD-------------------------WSAKQ--TYIALGNMLLAAAMLGIDsCPIEGF---DPAKLDEILGLDEKGYkI 132

                         170       180
                  ....*....|....*....|.
gi 1848022117 162 VLGLAIGKPDQDPALKPRMPR 182
Cdd:cd02149   133 SVMVAFGYRSEEKLPKSRKPL 153
nitroreductase cd03370
uncharacterized nitroreductase family proteins; Nitroreductase family containing Thermus ...
 13-173 2.10e-08

uncharacterized nitroreductase family proteins; Nitroreductase family containing Thermus thermophilus NADH oxidase and other, uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380327 [Multi-domain]  Cd Length: 191  Bit Score: 52.71  E-value: 2.10e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  13 RRSIRKFKPKPLSDDIVETLETVAQHAASSQFLNDWSAIRITDPAAKKRLAEIG-GQPYIATAPLLYVFV---------L 82
Cdd:cd03370     8 RRSIRKYTQEPVPDEDLREILRLAGLAPSAWNIQPWRFVVVRDAELKEQLQAAAyGQAQVTSAPAVIVIYsdmedalanL 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  83 DEHRNAAIAASKGVETASdefTLNGSY-RYTQAQ------NDAVLALHAMETAAYSLSLGCVILGSLlnDVPALIDLLNL 155
Cdd:cd03370    88 EETIHPGLSEERRQREAA---GLRGAFgKMSVEQrgqwglAQANIALGFLLLAAQSLGYDTSPMLGF--DPEKVKALLGL 162

                         170
                  ....*....|....*....
gi 1848022117 156 PEY-TYPVLgLAIGKPDQD 173
Cdd:cd03370   163 PEHvTIAAL-VALGKPAEE 180
nitroreductase cd20609
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 12-168 3.24e-08

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers.


Pssm-ID: 380330 [Multi-domain]  Cd Length: 145  Bit Score: 51.24  E-value: 3.24e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  12 ERRSIRKFKPKPLSDDIVET-LET--VAQHAASSQflnDWSAIRITDPAAKKRLAEIGgqPYIATAPlLYVFVLDEHRNA 88
Cdd:cd20609     8 KRYSVRKFSDKPVEKEKLDKiLEAgrLAPTAVNYQ---PQRILVVRSEEALEKLAKAT--PRFFGAP-LVIVVCYDKDES 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  89 AIAASKGVETAsDEftlngsyrytqaqnDAVLAL-HAMeTAAYSLSLGCVILGSLlnDVPALIDLLNLPEYTYPVLGLAI 167
Cdd:cd20609    82 WKRPYDGKDSG-DI--------------DAAIVAtHMM-LAATELGLGTCWVGNF--DPEKVREAFNLPENLEPVAILPL 143


                  .
gi 1848022117 168 G 168
Cdd:cd20609   144 G 144
iodotyrosine_dehalogenase cd02144
iodotyrosine dehalogenase; Iodotyrosine dehalogenase catalyzes the removal of iodine from the ...
 13-182 1.15e-04

iodotyrosine dehalogenase; Iodotyrosine dehalogenase catalyzes the removal of iodine from the 3, 5 positions of L-tyosine in thyroid, liver and kidney, using NADPH as electron donor. This enzyme is a homolog of the nitroreductase family. These enzymes are usually homodimers.


Pssm-ID: 380320  Cd Length: 192  Bit Score: 41.75  E-value: 1.15e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  13 RRSIRKFKPKPLSDDIVETLETVAQHAASSQFLNDWSAIRITDPAAKKRLAEI-------------GGQ----------- 68
Cdd:cd02144     8 RRSVRDFSSEPVPREVIENAIRTAGTAPSGANTQPWTFVVVSDPEIKRKIREAaeeeekefyekrmGEEwvwdlkplgtn 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  69 ---PYIATAPLLyvfvldehrnaaIAASKGVETASDEFTLNGSYRYTQAQNDAV----LALHAMEtaayslslgcviLGS 141
Cdd:cd02144    88 wekPYLTEAPYL------------IVVFKQKYGVLPDGKKKKHYYNEESVGIAVgillAALHNAG------------LVT 143

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....
gi 1848022117 142 LL---NDVPALIDLLNLPEYTYPVLGLAIGKPDQDpALKPRMPR 182
Cdd:cd02144   144 LThtpSPMPFLRDLLGRPKNEKPLLLLPVGYPAED-ATVPDLKR 186
nitroreductase cd20610
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 12-65 1.61e-03

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380331 [Multi-domain]  Cd Length: 167  Bit Score: 38.03  E-value: 1.61e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1848022117  12 ERRSIRKFKPKPLSDDIVETLETVAQHAASSQFLNDWSAIRITDPAAKKRLAEI 65
Cdd:cd20610     3 KRRSIRKFKPDPVPKEDIEKILEAANWAPSGMNRQNWEFVVVKGGEKIEKIGIS 56
TdsD-like cd02138
nitroreductase similar to Burkholderia pseudomallei TdsD; A subfamily of the nitroreductase ...
 10-176 1.96e-03

nitroreductase similar to Burkholderia pseudomallei TdsD; A subfamily of the nitroreductase family containing uncharacterized proteins that are similar to Burkholderia pseudomallei TdsD, may be involved in the processing of organosulfur compounds. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380315 [Multi-domain]  Cd Length: 174  Bit Score: 37.91  E-value: 1.96e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  10 LLERRSIRKFKPKPLSDDIVETLETVAQHAASSqfLND--WS---AIRITDPAAKKR--LAEiGGQPYIATAPLLyVFVL 82
Cdd:cd02138     2 IAERWSPRAFSPEPISEEDLLSLFEAARWAPSC--FNEqpWRfvvARRDTEAFEKLLdlLAE-GNQSWAKNAPVL-IVVL 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1848022117  83 dehrnaaiaaskgvetASDEFTLNGSYRYTqAQNDAVLALHAMETAAYSLSLGCVILGSLlnDVPALIDLLNLPEYTYPV 162
Cdd:cd02138    78 ----------------AKTEFDHNGKPNRY-ALFDTGAAVANLALQATALGLVVHQMAGF--DPEKAKEALGIPDEYEPI 138

                         170
                  ....*....|....
gi 1848022117 163 LGLAIGKPDQDPAL 176
Cdd:cd02138   139 TMIAIGYPGDPESL 152
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH