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Conserved domains on  [gi|172044036|gb|ACB69909|]
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membrane protein M [SARS coronavirus BJ182-12]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
SARS-like-CoV_M cd21569
Membrane (or Matrix) protein from Severe acute respiratory syndrome (SARS) coronavirus, ...
 4-221 1.73e-146

Membrane (or Matrix) protein from Severe acute respiratory syndrome (SARS) coronavirus, SARS-CoV-2, and related betacoronaviruses in the B lineage; This group contains the Membrane (M) protein of Severe acute respiratory syndrome coronavirus (SARS-CoV), SARS-CoV-2 (also known as 2019 novel CoV (2019-nCoV) or COVID-19 virus), and related proteins from betacoronaviruses in the sarbecovirus subgenera (B lineage). There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the Orf1ab (a large polyprotein known as replicase/protease); all are required to produce a structurally complete viral particle. The M protein, a triple-spanning membrane protein, is the most abundant protein in the virion. It plays a central role in virion assembly and morphogenesis, and it defines the shape of the viral envelope. It is regarded as the central organizer of CoV assembly, interacting with all other major coronaviral structural proteins and turning cellular membranes into workshops where virus and host factors come together to make new virus particles. While homotypic interactions between the M proteins are the major driving force behind virion envelope formation, it needs to interact with other coronaviral structural proteins for complete virion formation. The interaction of the Spike protein with M is not required for the assembly process. However, binding of M to N protein stabilizes the nucleocapsid (N protein-RNA complex), as well as the internal core of virions, and thereby promotes completion of viral assembly. Thus, the M protein, and its interactions with other structural proteins, is necessary for the production and release of virus-like particles.


:

Pssm-ID: 394855  Cd Length: 218  Bit Score: 406.70  E-value: 1.73e-146
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036   4 NGTITVEELKQLLEQWNLVIGFLCLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAVYRINWVTGGIAIAM 83
Cdd:cd21569    1 NGTITVEELKQLLEQWNLVIGFLFLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAVYRINWVTGGIAIAM 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036  84 ACIVGLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTIVTRPLMESELVIGAVIIRGHLRMAGHSLGRCDIKDL 163
Cdd:cd21569   81 ACIVGLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTILTRPLMESELVIGAVIIRGHLRMAGHSLGRCDIKDL 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 172044036 164 PKEITVATSRTLSYYKLGASQRVGTDSGFAAYNRYRIGNYKLNTDHAGSNDNIALLVQ 221
Cdd:cd21569  161 PKEITVATSRTLSYYKLGASQRVGTDSGFAAYNRYRIGNYKLNTDHSGSNDNIALLVQ 218
 
Name Accession Description Interval E-value
SARS-like-CoV_M cd21569
Membrane (or Matrix) protein from Severe acute respiratory syndrome (SARS) coronavirus, ...
 4-221 1.73e-146

Membrane (or Matrix) protein from Severe acute respiratory syndrome (SARS) coronavirus, SARS-CoV-2, and related betacoronaviruses in the B lineage; This group contains the Membrane (M) protein of Severe acute respiratory syndrome coronavirus (SARS-CoV), SARS-CoV-2 (also known as 2019 novel CoV (2019-nCoV) or COVID-19 virus), and related proteins from betacoronaviruses in the sarbecovirus subgenera (B lineage). There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the Orf1ab (a large polyprotein known as replicase/protease); all are required to produce a structurally complete viral particle. The M protein, a triple-spanning membrane protein, is the most abundant protein in the virion. It plays a central role in virion assembly and morphogenesis, and it defines the shape of the viral envelope. It is regarded as the central organizer of CoV assembly, interacting with all other major coronaviral structural proteins and turning cellular membranes into workshops where virus and host factors come together to make new virus particles. While homotypic interactions between the M proteins are the major driving force behind virion envelope formation, it needs to interact with other coronaviral structural proteins for complete virion formation. The interaction of the Spike protein with M is not required for the assembly process. However, binding of M to N protein stabilizes the nucleocapsid (N protein-RNA complex), as well as the internal core of virions, and thereby promotes completion of viral assembly. Thus, the M protein, and its interactions with other structural proteins, is necessary for the production and release of virus-like particles.


Pssm-ID: 394855  Cd Length: 218  Bit Score: 406.70  E-value: 1.73e-146
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036   4 NGTITVEELKQLLEQWNLVIGFLCLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAVYRINWVTGGIAIAM 83
Cdd:cd21569    1 NGTITVEELKQLLEQWNLVIGFLFLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAVYRINWVTGGIAIAM 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036  84 ACIVGLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTIVTRPLMESELVIGAVIIRGHLRMAGHSLGRCDIKDL 163
Cdd:cd21569   81 ACIVGLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTILTRPLMESELVIGAVIIRGHLRMAGHSLGRCDIKDL 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 172044036 164 PKEITVATSRTLSYYKLGASQRVGTDSGFAAYNRYRIGNYKLNTDHAGSNDNIALLVQ 221
Cdd:cd21569  161 PKEITVATSRTLSYYKLGASQRVGTDSGFAAYNRYRIGNYKLNTDHSGSNDNIALLVQ 218
CoV_M pfam01635
Coronavirus M matrix/glycoprotein; This family consists of various coronavirus matrix proteins ...
 16-215 2.70e-70

Coronavirus M matrix/glycoprotein; This family consists of various coronavirus matrix proteins which are transmembrane glycoproteins. The membrane (M) protein is the most abundant structural protein and defines the shape of the viral envelope, being the central organizer of coronavirus assembly. M proteins play a critical role in protein-protein interactions (as well as protein-RNA interactions) since virus-like particle (VLP) formation in many CoVs requires only the M and envelope (E) proteins for efficient virion assembly. Interaction of spike (S) with M is necessary for retention of S in the ER-Golgi intermediate compartment (ERGIC)/Golgi complex and its incorporation into new virions; binding of M to nucleocapsid (N) proteins stabilizes the nucleocapsid. Together, M and E protein make up the viral envelope and their interaction is sufficient for the production and release of virus-like particles (VLPs).


Pssm-ID: 460275  Cd Length: 208  Bit Score: 213.30  E-value: 2.70e-70
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036   16 LEQWNLVIGFLCLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAV--YRINWVTGGIAIAMACIVGLMWLS 93
Cdd:pfam01635   3 LREWNFAWSIILTLLIVVLQYGYASRSRFLYVLKMILLWLLWPLTLALSIFNAVasYNVNYVYFGFSIIFAVVTFVLWLM 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036   94 YFVASFRLFARTRSMWSFNPETNILLNVPLRGTIVTRPLMESELVIGAVIIRGHLRMAGHSLGR-CDIKDLPKEITVATS 172
Cdd:pfam01635  83 YFVNSIRLYRRTGSWWSFNPETNALLCVSVLGRTVTRPLDEDPTGITLTLLSGTLYVEGQKLARgVSVESLPNYVTVAKP 162

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....
gi 172044036  173 RTLSYY-KLGASQRVGTDSGFAAYNRYRIGNYKLNTDHAGSNDN 215
Cdd:pfam01635 163 STTIHYlKKGKSDNVGSNTGWAIYVRSKAGNYSLPSTTTGKLEE 206
 
Name Accession Description Interval E-value
SARS-like-CoV_M cd21569
Membrane (or Matrix) protein from Severe acute respiratory syndrome (SARS) coronavirus, ...
 4-221 1.73e-146

Membrane (or Matrix) protein from Severe acute respiratory syndrome (SARS) coronavirus, SARS-CoV-2, and related betacoronaviruses in the B lineage; This group contains the Membrane (M) protein of Severe acute respiratory syndrome coronavirus (SARS-CoV), SARS-CoV-2 (also known as 2019 novel CoV (2019-nCoV) or COVID-19 virus), and related proteins from betacoronaviruses in the sarbecovirus subgenera (B lineage). There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the Orf1ab (a large polyprotein known as replicase/protease); all are required to produce a structurally complete viral particle. The M protein, a triple-spanning membrane protein, is the most abundant protein in the virion. It plays a central role in virion assembly and morphogenesis, and it defines the shape of the viral envelope. It is regarded as the central organizer of CoV assembly, interacting with all other major coronaviral structural proteins and turning cellular membranes into workshops where virus and host factors come together to make new virus particles. While homotypic interactions between the M proteins are the major driving force behind virion envelope formation, it needs to interact with other coronaviral structural proteins for complete virion formation. The interaction of the Spike protein with M is not required for the assembly process. However, binding of M to N protein stabilizes the nucleocapsid (N protein-RNA complex), as well as the internal core of virions, and thereby promotes completion of viral assembly. Thus, the M protein, and its interactions with other structural proteins, is necessary for the production and release of virus-like particles.


Pssm-ID: 394855  Cd Length: 218  Bit Score: 406.70  E-value: 1.73e-146
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036   4 NGTITVEELKQLLEQWNLVIGFLCLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAVYRINWVTGGIAIAM 83
Cdd:cd21569    1 NGTITVEELKQLLEQWNLVIGFLFLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAVYRINWVTGGIAIAM 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036  84 ACIVGLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTIVTRPLMESELVIGAVIIRGHLRMAGHSLGRCDIKDL 163
Cdd:cd21569   81 ACIVGLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTILTRPLMESELVIGAVIIRGHLRMAGHSLGRCDIKDL 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 172044036 164 PKEITVATSRTLSYYKLGASQRVGTDSGFAAYNRYRIGNYKLNTDHAGSNDNIALLVQ 221
Cdd:cd21569  161 PKEITVATSRTLSYYKLGASQRVGTDSGFAAYNRYRIGNYKLNTDHSGSNDNIALLVQ 218
betaCoV_M cd21565
betacoronavirus Membrane (or Matrix) protein; This subfamily contains the Membrane (M) protein ...
 12-220 9.64e-112

betacoronavirus Membrane (or Matrix) protein; This subfamily contains the Membrane (M) protein of betacoronaviruses including the M proteins from three highly pathogenic human coronaviruses (CoVs) such as Middle East respiratory syndrome (MERS)-related CoV, severe acute respiratory syndrome (SARS) CoV, and SARS-CoV-2, also known as 2019 novel CoV (2019-nCoV) or COVID-19 virus. There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the Orf1ab (a large polyprotein known as replicase/protease); all are required to produce a structurally complete viral particle. The M protein, a triple-spanning membrane protein, is the most abundant protein in the virion. It plays a central role in virion assembly and morphogenesis, and it defines the shape of the viral envelope. It is regarded as the central organizer of CoV assembly, interacting with all other major coronaviral structural proteins and turning cellular membranes into workshops where virus and host factors come together to make new virus particles. While homotypic interactions between the M proteins are the major driving force behind virion envelope formation, it needs to interact with other coronaviral structural proteins for complete virion formation. The interaction of the Spike protein with M is not required for the assembly process. However, binding of M to N protein stabilizes the nucleocapsid (N protein-RNA complex), as well as the internal core of virions, and thereby promotes completion of viral assembly. Thus, the M protein, and its interactions with other structural proteins, is necessary for the production and release of virus-like particles.


Pssm-ID: 394851  Cd Length: 208  Bit Score: 318.56  E-value: 9.64e-112
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036  12 LKQLLEQWNLVIGFLCLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAVYRINWVTGGIAIAMACIVGLMW 91
Cdd:cd21565    1 IIAIIKDWNFAWSIIFLLITIVLQYGYPSRSMTVYVIKMFVLWLLWPLSIALSVFAAVYPINLVSQGISGIFAAISALMW 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036  92 LSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTIVTRPLMESELVIGAVIIRGHLRMAGHSLGRCDIKDLPKEITVAT 171
Cdd:cd21565   81 LSYFVQSIRLFRRTGSWWSFNPETNCLLNVPIGGTTVVRPLVEDSTSITAVVTRGHLKMAGMHLGRCDYDRLPNEVTVAK 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*....
gi 172044036 172 SRTLSYYKLGASQRVGTDSGFAAYNRYRIGNYKLNTDhAGSNDNIALLV 220
Cdd:cd21565  161 PNVLIAYKMVKRQRYGTNSGVAIYHRYKAGNYRVPPT-ITSDDELALLR 208
CoV_M cd21529
coronavirus Membrane (or Matrix) protein; This family contains the Membrane (M) protein of ...
 12-205 1.66e-87

coronavirus Membrane (or Matrix) protein; This family contains the Membrane (M) protein of coronaviruses (CoVs) including three highly pathogenic human CoVs such as Middle East respiratory syndrome (MERS)-related CoV, severe acute respiratory syndrome (SARS) CoV, and SARS-CoV-2, also known as 2019 novel CoV (2019-nCoV) or COVID-19 virus. There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the Orf1ab (a large polyprotein known as replicase/protease); all are required to produce a structurally complete viral particle. The M protein, a triple-spanning membrane protein, is the most abundant protein in the virion. It plays a central role in virion assembly and morphogenesis, and it defines the shape of the viral envelope. It is regarded as the central organizer of CoV assembly, interacting with all other major coronaviral structural proteins and turning cellular membranes into workshops where virus and host factors come together to make new virus particles. While homotypic interactions between the M proteins are the major driving force behind virion envelope formation, it needs to interact with other coronaviral structural proteins for complete virion formation. The interaction of the Spike protein with M is not required for the assembly process. However, binding of M to N protein stabilizes the nucleocapsid (N protein-RNA complex), as well as the internal core of virions, and thereby promotes completion of viral assembly. Thus, the M protein, and its interactions with other structural proteins, is necessary for the production and release of virus-like particles.


Pssm-ID: 394849 [Multi-domain]  Cd Length: 198  Bit Score: 256.66  E-value: 1.66e-87
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036  12 LKQLLEQWNLVIGFLCLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAVYRIN--WVTGGIAIAMACIVGL 89
Cdd:cd21529    1 SVSLLEWWNLVLIIILLALIAILQFGYATRSRMIYIIKLIVLWLLWPLTLAVTIWAAVYHGNskDAVFIISIIMAVLTFL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036  90 MWLSYFVASFRLFARTRSMWSFNPETNILLNVPL-RGTIVTRPLMESELVIGAVIIRGHLRMAGHSLGRCDIKD-LPKEI 167
Cdd:cd21529   81 MWLKYWIDSFRLFMRTRSAWSFNPESNALAGIMDpMGQWRCIPIDHMPPVISPVIKHGVLYLHGQELATGVSVHsLPKGI 160

                        170       180       190
                 ....*....|....*....|....*....|....*...
gi 172044036 168 TVATSRTLSYYKLGASQRVGTDSGFAAYNRYRIGNYKL 205
Cdd:cd21529  161 TVATPSTTFIYTLVKRIESTNDRGFAVYIYVKHGASNA 198
CoV_M pfam01635
Coronavirus M matrix/glycoprotein; This family consists of various coronavirus matrix proteins ...
 16-215 2.70e-70

Coronavirus M matrix/glycoprotein; This family consists of various coronavirus matrix proteins which are transmembrane glycoproteins. The membrane (M) protein is the most abundant structural protein and defines the shape of the viral envelope, being the central organizer of coronavirus assembly. M proteins play a critical role in protein-protein interactions (as well as protein-RNA interactions) since virus-like particle (VLP) formation in many CoVs requires only the M and envelope (E) proteins for efficient virion assembly. Interaction of spike (S) with M is necessary for retention of S in the ER-Golgi intermediate compartment (ERGIC)/Golgi complex and its incorporation into new virions; binding of M to nucleocapsid (N) proteins stabilizes the nucleocapsid. Together, M and E protein make up the viral envelope and their interaction is sufficient for the production and release of virus-like particles (VLPs).


Pssm-ID: 460275  Cd Length: 208  Bit Score: 213.30  E-value: 2.70e-70
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036   16 LEQWNLVIGFLCLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAV--YRINWVTGGIAIAMACIVGLMWLS 93
Cdd:pfam01635   3 LREWNFAWSIILTLLIVVLQYGYASRSRFLYVLKMILLWLLWPLTLALSIFNAVasYNVNYVYFGFSIIFAVVTFVLWLM 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036   94 YFVASFRLFARTRSMWSFNPETNILLNVPLRGTIVTRPLMESELVIGAVIIRGHLRMAGHSLGR-CDIKDLPKEITVATS 172
Cdd:pfam01635  83 YFVNSIRLYRRTGSWWSFNPETNALLCVSVLGRTVTRPLDEDPTGITLTLLSGTLYVEGQKLARgVSVESLPNYVTVAKP 162

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....
gi 172044036  173 RTLSYY-KLGASQRVGTDSGFAAYNRYRIGNYKLNTDHAGSNDN 215
Cdd:pfam01635 163 STTIHYlKKGKSDNVGSNTGWAIYVRSKAGNYSLPSTTTGKLEE 206
batCoV_HKU9-like_M cd21570
Membrane (or Matrix) protein from bat coronavirus HKU9 and related betacoronaviruses in the D ...
 4-220 1.34e-65

Membrane (or Matrix) protein from bat coronavirus HKU9 and related betacoronaviruses in the D lineage; This group contains the Membrane (M) protein of Rousettus bat coronavirus HKU9, and similar proteins from betacoronaviruses in the nobecovirus subgenera (D lineage). There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the Orf1ab (a large polyprotein known as replicase/protease); all are required to produce a structurally complete viral particle. The M protein, a triple-spanning membrane protein, is the most abundant protein in the virion. It plays a central role in virion assembly and morphogenesis, and it defines the shape of the viral envelope. It is regarded as the central organizer of CoV assembly, interacting with all other major coronaviral structural proteins and turning cellular membranes into workshops where virus and host factors come together to make new virus particles. While homotypic interactions between the M proteins are the major driving force behind virion envelope formation, it needs to interact with other coronaviral structural proteins for complete virion formation. The interaction of the Spike protein with M is not required for the assembly process. However, binding of M to N protein stabilizes the nucleocapsid (N protein-RNA complex), as well as the internal core of virions, and thereby promotes completion of viral assembly. Thus, the M protein, and its interactions with other structural proteins, is necessary for the production and release of virus-like particles.


Pssm-ID: 394856  Cd Length: 217  Bit Score: 202.01  E-value: 1.34e-65
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036   4 NGTITVEELKQLLEQWNLVIGFLCLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAVYRINWVTGGIAIAM 83
Cdd:cd21570    1 NSTFSREEVFAAVKDWNFAVSVLLLFITILLQWGYPSRCKPLWVVKMFILWLLWPLSIAAAVFAAVYPINKVAFGFAIAF 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036  84 ACIVGLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTIVTRPLMESELVIGAVIIRGHLRMAGHSLGRCDIKDL 163
Cdd:cd21570   81 ACISGLMWLSYFISSFRLLCRTGSAWSFMPETNMLLNVPLLGRTVTRPILADSPAIQFLLLRGEIQFDGFKLGRCDPGEM 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 172044036 164 PKEITVATSRTLSYYKLGASQRVGTDSGFAAYNRYRIGNYKL-NTDHAGsnDNIALLV 220
Cdd:cd21570  161 PDIVTVAKPASLHWYKKALTRNIGPKSAIIVYIKYKVGNHRVqNTNEDG--ERLAMFV 216
MERS-like-CoV_M cd21567
Membrane (or Matrix) protein from Middle East respiratory syndrome-related coronavirus and ...
 4-204 1.98e-63

Membrane (or Matrix) protein from Middle East respiratory syndrome-related coronavirus and related betacoronaviruses in the C lineage; This group contains the Membrane (M) protein of Middle East respiratory syndrome (MERS)-related CoV, bat-CoV HKU5, and similar proteins from betacoronaviruses in the merbecovirus subgenera (C lineage). There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the Orf1ab (a large polyprotein known as replicase/protease); all are required to produce a structurally complete viral particle. The M protein, a triple-spanning membrane protein, is the most abundant protein in the virion. It plays a central role in virion assembly and morphogenesis, and it defines the shape of the viral envelope. It is regarded as the central organizer of CoV assembly, interacting with all other major coronaviral structural proteins and turning cellular membranes into workshops where virus and host factors come together to make new virus particles. While homotypic interactions between the M proteins are the major driving force behind virion envelope formation, it needs to interact with other coronaviral structural proteins for complete virion formation. The interaction of the Spike protein with M is not required for the assembly process. However, binding of M to N protein stabilizes the nucleocapsid (N protein-RNA complex), as well as the internal core of virions, and thereby promotes completion of viral assembly. Thus, the M protein, and its interactions with other structural proteins, is necessary for the production and release of virus-like particles.


Pssm-ID: 394853  Cd Length: 216  Bit Score: 196.26  E-value: 1.98e-63
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036   4 NGTITVEELKQLLEQWNLVIGFLCLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAVYRINWVTGGIAIAM 83
Cdd:cd21567    2 NMTLTTEQIIAIIKDWNFAWSIIFLLITIVLQYGYPSRSMTVYVFKMFVLWLLWPSSMALSIFSAVYPIDLASQIISGIL 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036  84 ACIVGLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTIVTRPLMESELVIGAVIIRGHLRMAGHSLGRCDIKDL 163
Cdd:cd21567   82 AAISAMMWISYFVQSIRLFMRTGSWWSFNPETNCLLNVPIGGTTVVRPLVEDSTSVTAVVTNGYLKMAGMHFGACDYDRL 161

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|.
gi 172044036 164 PKEITVATSRTLSYYKLGASQRVGTDSGFAAYNRYRIGNYK 204
Cdd:cd21567  162 PNEVTVAKPNVLIALKMVKRQSYGTNSGVAIYHRYKAGNYR 202
HCoV-like_M cd21568
Membrane (or Matrix) protein from human coronavirus and related betacoronaviruses in the A ...
 8-219 1.06e-54

Membrane (or Matrix) protein from human coronavirus and related betacoronaviruses in the A lineage; This group contains the Membrane (M) protein of human coronaviruses (HCoVs), HCoV-OC43 and HCoV-HKU1, and similar proteins from betacoronaviruses in the embecovirus subgenera (A lineage). There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the Orf1ab (a large polyprotein known as replicase/protease); all are required to produce a structurally complete viral particle. The M protein, a triple-spanning membrane protein, is the most abundant protein in the virion. It plays a central role in virion assembly and morphogenesis, and it defines the shape of the viral envelope. It is regarded as the central organizer of CoV assembly, interacting with all other major coronaviral structural proteins and turning cellular membranes into workshops where virus and host factors come together to make new virus particles. While homotypic interactions between the M proteins are the major driving force behind virion envelope formation, it needs to interact with other coronaviral structural proteins for complete virion formation. The interaction of the Spike protein with M is not required for the assembly process. However, binding of M to N protein stabilizes the nucleocapsid (N protein-RNA complex), as well as the internal core of virions, and thereby promotes completion of viral assembly. Thus, the M protein, and its interactions with other structural proteins, is necessary for the production and release of virus-like particles.


Pssm-ID: 394854  Cd Length: 220  Bit Score: 174.21  E-value: 1.06e-54
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036   8 TVEELKQLLEQWNLVIGFLCLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAVYRINWVTGGIAIAMACIV 87
Cdd:cd21568    8 TADEAIKFLKEWNFSLGIILLFITIILQFGYTSRSMFVYVIKMIILWLMWPLTIILTIFNCVYALNNVYLGFSIVFTIVS 87

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036  88 GLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTIVTRPLMESELVIGAVIIRGHLRMAGHSLGR-CDIKDLPKE 166
Cdd:cd21568   88 IIMWIVYFVNSIRLFIRTGSWWSFNPETNNLMCIDMKGTMYVRPIIEDYHTLTATIIRGHLYIQGVKLGTgYSLSDLPAY 167

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|...
gi 172044036 167 ITVATSRTLSYYKLGASQRVGTDSGFAAYNRYRIGNYKLNTDHaGSNDNIALL 219
Cdd:cd21568  168 VTVAKVSHLCTYKRAFLDKIDDTSGFAVYVKSKVGNYRLPSNK-GSGMDTALL 219
alphaCoV_M cd21564
alphacoronavirus Membrane (or Matrix) protein; This subfamily contains the Membrane (M) ...
 5-203 7.25e-44

alphacoronavirus Membrane (or Matrix) protein; This subfamily contains the Membrane (M) protein of alphacoronaviruses including human coronaviruses (HCoVs), HCoV-229E and HCoV-NL63. There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the Orf1ab (a large polyprotein known as replicase/protease); all are required to produce a structurally complete viral particle. The M protein, a triple-spanning membrane protein, is the most abundant protein in the virion. It plays a central role in virion assembly and morphogenesis, and it defines the shape of the viral envelope. It is regarded as the central organizer of CoV assembly, interacting with all other major coronaviral structural proteins and turning cellular membranes into workshops where virus and host factors come together to make new virus particles. While homotypic interactions between the M proteins are the major driving force behind virion envelope formation, it needs to interact with other coronaviral structural proteins for complete virion formation. The interaction of the Spike protein with M is not required for the assembly process. However, binding of M to N protein stabilizes the nucleocapsid (N protein-RNA complex), as well as the internal core of virions, and thereby promotes completion of viral assembly. Thus, the M protein, and its interactions with other structural proteins, is necessary for the production and release of virus-like particles.


Pssm-ID: 394850  Cd Length: 218  Bit Score: 146.26  E-value: 7.25e-44
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036   5 GTITVEELKQLLEQWNLVIGFLCLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAV--YRINWVTGGIAIA 82
Cdd:cd21564    1 GCIPVGEVIWHLRNWNFSWNIILTIFIVVLQYGHYKYSWFLYGLKMLILWLLWPLVLALSIFDAWasFQVNWVFFAFSIL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036  83 MACIVGLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTIVTRPLMESELVIGAVIIRGHLRMAGHSL-GRCDIK 161
Cdd:cd21564   81 MACITLVLWIMYFVNSIRLYRRTHSWWSFNPETNAILCVSVLGRSYCLPVLGAPTGVTLTLLSGTLLVEGFKVaTGVQVD 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|...
gi 172044036 162 DLPKEITVAT-SRTLSYYKLGASQRVGTDSGFAAYNRYRIGNY 203
Cdd:cd21564  161 QLPKYVTVAKpSTTIVYTRVGRSLNASSATGWAFYVRSKHGDY 203
gammaCoV_M cd21566
gammacoronavirus Membrane (or Matrix) protein; This subfamily contains the Membrane (M) ...
 6-179 2.26e-35

gammacoronavirus Membrane (or Matrix) protein; This subfamily contains the Membrane (M) protein of gammacoronavirus including avian infectious bronchitis virus (IBV). There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the Orf1ab (a large polyprotein known as replicase/protease); all are required to produce a structurally complete viral particle. The M protein, a triple-spanning membrane protein, is the most abundant protein in the virion. It plays a central role in virion assembly and morphogenesis, and it defines the shape of the viral envelope. It is regarded as the central organizer of CoV assembly, interacting with all other major coronaviral structural proteins and turning cellular membranes into workshops where virus and host factors come together to make new virus particles. While homotypic interactions between the M proteins are the major driving force behind virion envelope formation, it needs to interact with other coronaviral structural proteins for complete virion formation. The interaction of the Spike protein with M is not required for the assembly process. However, binding of M to N protein stabilizes the nucleocapsid (N protein-RNA complex), as well as the internal core of virions, and thereby promotes completion of viral assembly. Thus, the M protein, and its interactions with other structural proteins, is necessary for the production and release of virus-like particles.


Pssm-ID: 394852  Cd Length: 212  Bit Score: 124.47  E-value: 2.26e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036   6 TITVEELKQLLEQWNLVIGFLCLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAVYRINwvTGGI--AIAM 83
Cdd:cd21566    2 TLDTEQAVELFKEYNLFITAFLLFLTILLQYGYATRSRFIYILKMIVLWCFWPLNIAVGVISCIYPPN--TGGLvaAIIL 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036  84 ACIVGLMWLSYFVASFRLFARTRSMWSFNPETN----ILLNvplRGTIVTRPLMESELVIGAVIIRGHLRMAGHSLGRCD 159
Cdd:cd21566   80 TVFACLSFVGYWIQSIRLFKRCRSWWSFNPESNavgsILLT---NGQQCNFAIESVPMVLSPIIKNGVLYCEGQWLAKCE 156

                        170       180
                 ....*....|....*....|
gi 172044036 160 IKDLPKEITVATSRTLSYYK 179
Cdd:cd21566  157 PDHLPKDIFVCTPDRRNIYR 176
deltaCoV_M cd21594
deltacoronavirus Membrane (or Matrix) protein; This subfamily contains the Membrane (M) ...
 17-218 4.66e-23

deltacoronavirus Membrane (or Matrix) protein; This subfamily contains the Membrane (M) protein of deltacoronaviruses including porcine deltacoronavirus and Bulbul coronavirus HKU11. There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and the Orf1ab (a large polyprotein known as replicase/protease); all are required to produce a structurally complete viral particle. The M protein, a triple-spanning membrane protein, is the most abundant protein in the virion. It plays a central role in virion assembly and morphogenesis, and it defines the shape of the viral envelope. It is regarded as the central organizer of CoV assembly, interacting with all other major coronaviral structural proteins and turning cellular membranes into workshops where virus and host factors come together to make new virus particles. While homotypic interactions between the M proteins are the major driving force behind virion envelope formation, it needs to interact with other coronaviral structural proteins for complete virion formation. The interaction of the Spike protein with M is not required for the assembly process. However, binding of M to N protein stabilizes the nucleocapsid (N protein-RNA complex), as well as the internal core of virions, and thereby promotes completion of viral assembly. Thus, the M protein, and its interactions with other structural proteins, is necessary for the production and release of virus-like particles.


Pssm-ID: 394857  Cd Length: 217  Bit Score: 92.51  E-value: 4.66e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036  17 EQWNLVIgFLCLAWIM--LLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAVYRINWVTGG---IAIAMACIVGLMW 91
Cdd:cd21594    5 EEWQIIV-FIIIIWALgvILQGGYATRNRMIYVIKLILLWLLQPFTLVVTIWTAVDNGSKKTDAvfiISIIFAVLTFVSW 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 172044036  92 LSYFVASFRLFARTRSMWSFNPETNILLNV--PLrGTIVTRPLMESELVIGAVIIRGHLRMAGHSLGR-CDIKDLPKEIT 168
Cdd:cd21594   84 LKYWYDSIRLVMKTKSAWSFSPESRLLAGImdPM-GNWRCIPIDHMAPILTPVVKHGKLKLHGQELATgISVTSPPKDMV 162

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 172044036 169 VATSRTLSYYKLGASQRVGTDSGFAA--YNRYRIGNYKLNTDHAGSNDNIAL 218
Cdd:cd21594  163 VVSPSDTFHYTLKKTIESTDDPDFAVliYQGDRASNAGLHTITTSKSGNARL 214
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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