RhoGAP_fLRG1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of fungal LRG1-like proteins. Yeast Lrg1p is required for efficient cell fusion, and mother-daughter cell separation, possibly through acting as a RhoGAP specifically regulating 1,3-beta-glucan synthesis. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  881 FGVALDYLVEKDGAESTHGVGPGALRIPALVDDAVSAMRQMDMSVEGVFRKNGNIRRLKDLAELIDNKY-EQVDLSKENP 959
Cdd:cd04397      1 FGVPLEILVEKFGADSTLGVGPGKLRIPALIDDIISAMRQMDMSVEGVFRKNGNIRRLKELTEEIDKNPtEVPDLSKENP 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  960 VQIAALLKKFLREMPDPLLTFKLHRLFVISQKISDPDKQKRILHLTCCLLPKAHRDTMEVLFAFLNWTSSFSHVDEETGS 1039
Cdd:cd04397     81 VQLAALLKKFLRELPDPLLTFKLYRLWISSQKIEDEEERKRVLHLVYCLLPKYHRDTMEVLFSFLKWVSSFSHIDEETGS 160

                          170       180       190       200       210
                   ....*....|....*....|....*....|....*....|....*....|...
gi 1556664380 1040 KMDIHNLATVITPNILYSNTKNGGM-DESFLAIEAVTALITYNDTMCEVPDDL 1091
Cdd:cd04397    161 KMDIHNLATVITPNILYSKTDNPNTgDEYFLAIEAVNYLIENNEEFCEVPDEL 213

The first LIM domain of Lrg1p, a LIM and RhoGap domain containing protein; The first LIM domain of Lrg1p, a LIM and RhoGap domain containing protein: The members of this family contain three tandem repeats of LIM domains and a Rho-type GTPase activating protein (RhoGap) domain. Lrg1p is a Rho1 GTPase-activating protein required for efficient cell fusion in yeast. Lrg1p-GAP domain strongly and specifically stimulates the GTPase activity of Rho1p, a regulator of beta (1-3)-glucan synthase in vitro. The LIM domain is 50-60 amino acids in size and shares two characteristic zinc finger motifs. The two zinc fingers contain eight conserved residues, mostly cysteines and histidines, which coordinately bond to two zinc atoms. LIM domains function as adaptors or scaffolds to support the assembly of multimeric protein.

                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*..
gi 1556664380  142 CKKCGETLTGQFVRALGGTFHLECFKCSDCGQIVASKFFPVDAEDGNGQYPLCETDY 198
Cdd:cd09391      1 CAKCGKPITGQFVRALGDVYHLDCFTCHDCGKPVASKFFPVDDPDTSEQVPLCETDY 57

LIM is a small protein-protein interaction domain, containing two zinc fingers; LIM domains are identified in a diverse group of proteins with wide variety of biological functions, including gene expression regulation, cell fate determination, cytoskeleton organization, tumor formation and development. LIM domains function as adaptors or scaffolds to support the assembly of multimeric protein complexes. They perform their functions through interactions with other protein partners. LIM domains are 50-60 amino acids in size and share two characteristic highly conserved zinc finger motifs. The two zinc fingers contain eight conserved residues, mostly cysteines and histidines, which coordinately bond to two zinc atoms. The consensus sequence of LIM domain has been defined as C-x(2)-C-x(16,23)-H-x(2)-[CH]-x(2)-C-x(2)-C-x(16,21)-C-x(2,3)-[CHD] (where X denotes any amino acid).

                           10        20        30
                   ....*....|....*....|....*....|..
gi 1556664380  266 CNGCHTAILKQFVEIFrngqNQHWHPECYMIH 297
Cdd:cd09338      1 CGGCNKPILENYISAL----NTQWHPECFVCR 28

RhoGAP_fLRG1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of fungal LRG1-like proteins. Yeast Lrg1p is required for efficient cell fusion, and mother-daughter cell separation, possibly through acting as a RhoGAP specifically regulating 1,3-beta-glucan synthesis. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  881 FGVALDYLVEKDGAESTHGVGPGALRIPALVDDAVSAMRQMDMSVEGVFRKNGNIRRLKDLAELIDNKY-EQVDLSKENP 959
Cdd:cd04397      1 FGVPLEILVEKFGADSTLGVGPGKLRIPALIDDIISAMRQMDMSVEGVFRKNGNIRRLKELTEEIDKNPtEVPDLSKENP 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  960 VQIAALLKKFLREMPDPLLTFKLHRLFVISQKISDPDKQKRILHLTCCLLPKAHRDTMEVLFAFLNWTSSFSHVDEETGS 1039
Cdd:cd04397     81 VQLAALLKKFLRELPDPLLTFKLYRLWISSQKIEDEEERKRVLHLVYCLLPKYHRDTMEVLFSFLKWVSSFSHIDEETGS 160

                          170       180       190       200       210
                   ....*....|....*....|....*....|....*....|....*....|...
gi 1556664380 1040 KMDIHNLATVITPNILYSNTKNGGM-DESFLAIEAVTALITYNDTMCEVPDDL 1091
Cdd:cd04397    161 KMDIHNLATVITPNILYSKTDNPNTgDEYFLAIEAVNYLIENNEEFCEVPDEL 213

GTPase-activator protein for Rho-like GTPases; GTPase activator proteins towards Rho/Rac/Cdc42-like small GTPases. etter domain limits and outliers.

                            10        20        30        40        50        60        70        80
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380   906 RIPALVDDAVSAMRQMDMSVEGVFRKNGNIRRLKDLAELIDN-KYEQVDLSKENPVQIAALLKKFLREMPDPLLTFKLHR 984
Cdd:smart00324    2 PIPIIVEKCIEYLEKRGLDTEGIYRVSGSKSRVKELRDAFDSgPDPDLDLSEYDVHDVAGLLKLFLRELPEPLITYELYE 81

                            90       100       110       120       130       140       150       160
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380   985 LFVISQKISDPDKQKRILHLTCCLLPKAHRDTMEVLFAFLNWTSSFSHVdeetgSKMDIHNLATVITPNILYSNTKN-GG 1063
Cdd:smart00324   82 EFIEAAKLEDETERLRALRELLSLLPPANRATLRYLLAHLNRVAEHSEE-----NKMTARNLAIVFGPTLLRPPDGEvAS 156

                           170
                    ....*....|....*..
gi 1556664380  1064 MDESFLAIEAVTALITY 1080
Cdd:smart00324  157 LKDIRHQNTVIEFLIEN 173

The first LIM domain of Lrg1p, a LIM and RhoGap domain containing protein; The first LIM domain of Lrg1p, a LIM and RhoGap domain containing protein: The members of this family contain three tandem repeats of LIM domains and a Rho-type GTPase activating protein (RhoGap) domain. Lrg1p is a Rho1 GTPase-activating protein required for efficient cell fusion in yeast. Lrg1p-GAP domain strongly and specifically stimulates the GTPase activity of Rho1p, a regulator of beta (1-3)-glucan synthase in vitro. The LIM domain is 50-60 amino acids in size and shares two characteristic zinc finger motifs. The two zinc fingers contain eight conserved residues, mostly cysteines and histidines, which coordinately bond to two zinc atoms. LIM domains function as adaptors or scaffolds to support the assembly of multimeric protein.

                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*..
gi 1556664380  142 CKKCGETLTGQFVRALGGTFHLECFKCSDCGQIVASKFFPVDAEDGNGQYPLCETDY 198
Cdd:cd09391      1 CAKCGKPITGQFVRALGDVYHLDCFTCHDCGKPVASKFFPVDDPDTSEQVPLCETDY 57

The third LIM domain of Lrg1p, a LIM and RhoGap domain containing protein; The third LIM domain of Lrg1p, a LIM and RhoGap domain containing protein: The members of this family contain three tandem repeats of LIM domains and a Rho-type GTPase activating protein (RhoGap) domain. Lrg1p is a Rho1 GTPase-activating protein required for efficient cell fusion in yeast. Lrg1p-GAP domain strongly and specifically stimulates the GTPase activity of Rho1p, a regulator of beta (1-3)-glucan synthase in vitro. The LIM domain is 50-60 amino acids in size and shares two characteristic zinc finger motifs. The two zinc fingers contain eight conserved residues, mostly cysteines and histidines, which coordinately bond to two zinc atoms. LIM domains function as adaptors or scaffolds to support the assembly of multimeric protein.

                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*...
gi 1556664380  515 CTACREPIDDECIILGERRWHLKPphLQCAACQKDLTVDLADGKWNETNNRVFCSSCA 572
Cdd:cd09393      1 CASCGKSIEDECIKFEDKRWHLKC--FTCSRCHREISSELSDAAFNNKDQRILCSNCS 56

Zinc-binding domain present in Lin-11, Isl-1, Mec-3; Zinc-binding domain family. Some LIM domains bind protein partners via tyrosine-containing motifs. LIM domains are found in many key regulators of developmental pathways.

                            10        20        30        40        50
                    ....*....|....*....|....*....|....*....|....*....|....
gi 1556664380   206 CHECGGALRGS--YITALDRKYHIEHFTCSVCPTVFGaQDSYYEHEGKVYCHYH 257
Cdd:smart00132    2 CAGCGKPIYGTerVLRALGKVWHPECFKCATCGKPLS-GDTFFEKDGKLYCKDC 54

LIM domain; This family represents two copies of the LIM structural domain.

                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*...
gi 1556664380  515 CTACREPIDDECIILG-ERRWHlkPPHLQCAACQKDLTvdlaDGKWNETNNRVFCSSC 571
Cdd:pfam00412    1 CAGCNRPIYDRELVRAlGKVWH--PECFRCAVCGKPLT----TGDFYEKDGKLYCKHD 52

RhoGAP_fLRG1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of fungal LRG1-like proteins. Yeast Lrg1p is required for efficient cell fusion, and mother-daughter cell separation, possibly through acting as a RhoGAP specifically regulating 1,3-beta-glucan synthesis. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  881 FGVALDYLVEKDGAESTHGVGPGALRIPALVDDAVSAMRQMDMSVEGVFRKNGNIRRLKDLAELIDNKY-EQVDLSKENP 959
Cdd:cd04397      1 FGVPLEILVEKFGADSTLGVGPGKLRIPALIDDIISAMRQMDMSVEGVFRKNGNIRRLKELTEEIDKNPtEVPDLSKENP 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  960 VQIAALLKKFLREMPDPLLTFKLHRLFVISQKISDPDKQKRILHLTCCLLPKAHRDTMEVLFAFLNWTSSFSHVDEETGS 1039
Cdd:cd04397     81 VQLAALLKKFLRELPDPLLTFKLYRLWISSQKIEDEEERKRVLHLVYCLLPKYHRDTMEVLFSFLKWVSSFSHIDEETGS 160

                          170       180       190       200       210
                   ....*....|....*....|....*....|....*....|....*....|...
gi 1556664380 1040 KMDIHNLATVITPNILYSNTKNGGM-DESFLAIEAVTALITYNDTMCEVPDDL 1091
Cdd:cd04397    161 KMDIHNLATVITPNILYSKTDNPNTgDEYFLAIEAVNYLIENNEEFCEVPDEL 213

GTPase-activator protein for Rho-like GTPases; GTPase activator proteins towards Rho/Rac/Cdc42-like small GTPases. etter domain limits and outliers.

                            10        20        30        40        50        60        70        80
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380   906 RIPALVDDAVSAMRQMDMSVEGVFRKNGNIRRLKDLAELIDN-KYEQVDLSKENPVQIAALLKKFLREMPDPLLTFKLHR 984
Cdd:smart00324    2 PIPIIVEKCIEYLEKRGLDTEGIYRVSGSKSRVKELRDAFDSgPDPDLDLSEYDVHDVAGLLKLFLRELPEPLITYELYE 81

                            90       100       110       120       130       140       150       160
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380   985 LFVISQKISDPDKQKRILHLTCCLLPKAHRDTMEVLFAFLNWTSSFSHVdeetgSKMDIHNLATVITPNILYSNTKN-GG 1063
Cdd:smart00324   82 EFIEAAKLEDETERLRALRELLSLLPPANRATLRYLLAHLNRVAEHSEE-----NKMTARNLAIVFGPTLLRPPDGEvAS 156

                           170
                    ....*....|....*..
gi 1556664380  1064 MDESFLAIEAVTALITY 1080
Cdd:smart00324  157 LKDIRHQNTVIEFLIEN 173

The second LIM domain of Lrg1p, a LIM and RhoGap domain containing protein; The second LIM domain of Lrg1p, a LIM and RhoGap domain containing protein: The members of this family contain three tandem repeats of LIM domains and a Rho-type GTPase activating protein (RhoGap) domain. Lrg1p is a Rho1 GTPase-activating protein required for efficient cell fusion in yeast. Lrg1p-GAP domain strongly and specifically stimulates the GTPase activity of Rho1p, a regulator of beta (1-3)-glucan synthase in vitro. The LIM domain is 50-60 amino acids in size and shares two characteristic zinc finger motifs. The two zinc fingers contain eight conserved residues, mostly cysteines and histidines, which coordinately bond to two zinc atoms. LIM domains function as adaptors or scaffolds to support the assembly of multimeric protein.

                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|...
gi 1556664380  206 CHECGGALRGSYITALDRKYHIEHFTCSVCPTVFGAQDSYYEHEGKVYCHYHY 258
Cdd:cd09392      1 CFKCGGALRGSYITALGRKYHVEHFTCSVCPTVFGPNDSYYEHEGKIYCHYHY 53

RhoGAP_ARHGAP6: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of ArhGAP6-like proteins. ArhGAP6 shows GAP activity towards RhoA, but not towards Cdc42 and Rac1. ArhGAP6 is often deleted in microphthalmia with linear skin defects syndrome (MLS); MLS is a severe X-linked developmental disorder. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  900 VGPGALRIPALVDDAVSAMRQMDMSVEGVFRKNGNIRRLKDLAELIDNKYEQVDLSKENPVQIAALLKKFLREMPDPLLT 979
Cdd:cd04376      2 LNPIARQVPRLVESCCQHLEKHGLQTVGIFRVGSSKKRVRQLREEFDRGIDVVLDENHSVHDVAALLKEFFRDMPDPLLP 81

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  980 FKLHRLFVISQKISdPDKQKRILHLTCCLLPKAHRDTMEVLFAFLNWTS--SFSHVDEE----TGSKMDIHNLATVITPN 1053
Cdd:cd04376     82 RELYTAFIGTALLE-PDEQLEALQLLIYLLPPCNCDTLHRLLKFLHTVAehAADSIDEDgqevSGNKMTSLNLATIFGPN 160

                          170       180       190       200
                   ....*....|....*....|....*....|....*....|....*..
gi 1556664380 1054 ILYSnTKNG---------GMDESFLAIEAVTALITYNDTMCEVPDDL 1091
Cdd:cd04376    161 LLHK-QKSGerefvqaslRIEESTAIINVVQTMIDNYEELFMVSPEL 206

RhoGAP-ARHGAP11A: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of ArhGAP11A-like proteins. The mouse homolog of human ArhGAP11A has been detected as a gene exclusively expressed in immature ganglion cells, potentially playing a role in retinal development. The exact function of ArhGAP11A is unknown. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  880 VFGVALDYLvekdgaesTHGVGPGALRIPALVDDAVSAMRQmDMSVEGVFRKNGNIRRLKDLAELIDNKyeQVDLSKENP 959
Cdd:cd04394      1 VFGVPLHSL--------PHSTVPEYGNVPKFLVDACTFLLD-HLSTEGLFRKSGSVVRQKELKAKLEGG--EACLSSALP 69

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  960 VQIAALLKKFLREMPDPLLTFKLHRLFVISQKISDPDKQKRILHLTCCLLPKAHRDTMEVLFAFLNWTSsfSHVDEetgS 1039
Cdd:cd04394     70 CDVAGLLKQFFRELPEPLLPYDLHEALLKAQELPTDEERKSATLLLTCLLPDEHVNTLRYFFSFLYDVA--QRCSE---N 144

                          170       180       190       200       210
                   ....*....|....*....|....*....|....*....|....*....|....*
gi 1556664380 1040 KMDIHNLATVITPNILYSNTKNGGMDESF---LAIEA--VTALITYNDTMCEVPD 1089
Cdd:cd04394    145 KMDSSNLAVIFAPNLFQSEEGGEKMSSSTekrLRLQAavVQTLIDNASNIGIVPD 199

RhoGAP_nadrin: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of Nadrin-like proteins. Nadrin, also named Rich-1, has been shown to be involved in the regulation of Ca2+-dependent exocytosis in neurons and recently has been implicated in tight junction maintenance in mammalian epithelium. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  878 KGVFGVALDYLVEKDGAEsthgvgpgalrIPALVDDAVSAMRQMDMSVEGVFRKNGNIRRLKDLAELIDNKYEQVDLSKE 957
Cdd:cd04386      2 KPVFGTPLEEHLKRTGRE-----------IALPIEACVMCLLETGMNEEGLFRVGGGASKLKRLKAALDAGTFSLPLDEF 70

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  958 N--PVQIAALLKKFLREMPDPLLTFKLHRLFVISQKISDPDKQKRILHLTCCLLPKAHRDTMEVLFAFLNWTSSFSHVDe 1035
Cdd:cd04386     71 YsdPHAVASALKSYLRELPDPLLTYNLYEDWVQAANKPDEDERLQAIWRILNKLPRENRDNLRYLIKFLSKLAQKSDEN- 149

                          170       180
                   ....*....|....*....|....*.
gi 1556664380 1036 etgsKMDIHNLATVITPNILYSNTKN 1061
Cdd:cd04386    150 ----KMSPSNIAIVLAPNLLWAKNEG 171

RhoGAP_FAM13A1a: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of FAM13A1, isoform a-like proteins. The function of FAM13A1a is unknown. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by up several orders of magnitude.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  880 VFGVALDYLveKDGAESTHGVgpgalriPALVDDAVSAMRQMDMSVEGVFRKNGNIRRLKDLAELIDNKyEQVDLSKENP 959
Cdd:cd04393      2 VFGVPLQEL--QQAGQPENGV-------PAVVRHIVEYLEQHGLEQEGLFRVNGNAETVEWLRQRLDSG-EEVDLSKEAD 71

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  960 V-QIAALLKKFLREMPDPLLTFKLH-RLFVISQKISDPDKQKRILHLTCCLLPKAHRDTMEVLFAFLNWTSSFSHVDeet 1037
Cdd:cd04393     72 VcSAASLLRLFLQELPEGLIPASLQiRLMQLYQDYNGEDEFGRKLRDLLQQLPPVNYSLLKFLCHFLSNVASQHHEN--- 148

                          170       180
                   ....*....|....*....|....*....
gi 1556664380 1038 gsKMDIHNLATVITPNILYSNTKNGGMDE 1066
Cdd:cd04393    149 --RMTAENLAAVFGPDVFHVYTDVEDMKE 175

RhoGAP_fRGD1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of fungal RGD1-like proteins. Yeast Rgd1 is a GAP protein for Rho3 and Rho4 and plays a role in low-pH response. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  881 FGVALDYLVEKDGAEsthgvgpgalrIPALVDDAVSAMRQMDMSVEGVFRKNGNIRRLKDLAELIDNKYEQVDLSK---- 956
Cdd:cd04398      1 FGVPLEDLILREGDN-----------VPNIVYQCIQAIENFGLNLEGIYRLSGNVSRVNKLKELFDKDPLNVLLISpedy 69

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  957 ENPVQ-IAALLKKFLREMPDPLLTFKLHRLFVISQKISDPDKQKRILHLTCCLLPKAHRDTMEVLFAFLNWTSSFSHVDe 1035
Cdd:cd04398     70 ESDIHsVASLLKLFFRELPEPLLTKALSREFIEAAKIEDESRRRDALHGLINDLPDANYATLRALMFHLARIKEHESVN- 148

                          170       180
                   ....*....|....*....|....*.
gi 1556664380 1036 etgsKMDIHNLATVITPNILYSNTKN 1061
Cdd:cd04398    149 ----RMSVNNLAIIWGPTLMNAAPDN 170

RhoGAP_DLC1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of DLC1-like proteins. DLC1 shows in vitro GAP activity towards RhoA and CDC42. Beside its C-terminal GAP domain, DLC1 also contains a SAM (sterile alpha motif) and a START (StAR-related lipid transfer action) domain. DLC1 has tumor suppressor activity in cell culture. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  878 KGVFGVALDYLVEKDGAEsthgvgpgalrIPALVDDAVSAMRQMDMSVEGVFRKNGNIRRLKDLAELIDNKYEQVDLSKE 957
Cdd:cd04375      2 KNVFGVPLLVNLQRTGQP-----------LPRSIQQAMRWLRNNALDQVGLFRKSGVKSRIQKLRSMIESSTDNVNYDGQ 70

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  958 NPVQIAALLKKFLREMPDPLLTFKLHRLFV-ISQKIsdPDKQkRILHLTCC--LLPKAHRDTMEVLFAFLNWTSSFSHVD 1034
Cdd:cd04375     71 QAYDVADMLKQYFRDLPEPLLTNKLSETFIaIFQYV--PKEQ-RLEAVQCAilLLPDENREVLQTLLYFLSDVAANSQEN 147

                          170       180       190       200       210       220       230
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1556664380 1035 EETGSkmdihNLATVITPNILYSNTKNGG---------------------MDESFLAIEAVTALITYNDTMCEVPDDL 1091
Cdd:cd04375    148 QMTAT-----NLAVCLAPSLFHLNTSRREnssparrmqrkkslgkpdqkeLSENKAAHQCLAYMIEECNTLFMVPKEM 220

RhoGAP_ARHGAP21: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of ArhGAP21-like proteins. ArhGAP21 is a multi-domain protein, containing RhoGAP, PH and PDZ domains, and is believed to play a role in the organization of the cell-cell junction complex. It has been shown to function as a GAP of Cdc42 and RhoA, and to interact with alpha-catenin and Arf6. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  906 RIPALVDDAVSAMRQMDMSVEGVFRKNGNIRRLKDLAELIDNKYEQVDLSKE--NPVQ-IAALLKKFLREMPDPLLTFKL 982
Cdd:cd04395     17 YVPLIVEVCCNIVEARGLETVGIYRVPGNNAAISALQEELNRGGFDIDLQDPrwRDVNvVSSLLKSFFRKLPEPLFTNEL 96

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  983 HRLFVISQKISDPDKQ----KRILHltccLLPKAHRDTMEVLFAFLNWTSSFSHVDeetgsKMDIHNLATVITPNILYSN 1058
Cdd:cd04395     97 YPDFIEANRIEDPVERlkelRRLIH----SLPDHHYETLKHLIRHLKTVADNSEVN-----KMEPRNLAIVFGPTLVRTS 167

                          170       180       190
                   ....*....|....*....|....*....|.
gi 1556664380 1059 TKN-----GGMDESFLAIEavtALITYNDTM 1084
Cdd:cd04395    168 DDNmetmvTHMPDQCKIVE---TLIQHYDWF 195

RhoGAP_ARHGAP27_15_12_9: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP27 (also called CAMGAP1), ARHGAP15, 12 and 9-like proteins; This subgroup of ARHGAPs are multidomain proteins that contain RhoGAP, PH, SH3 and WW domains. Most members that are studied show GAP activity towards Rac1, some additionally show activity towards Cdc42. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  881 FGVALDYLVEKDGAesthgvgpgalRIPALVDDAVSAMRQMDMSVEGVFRKNGNIRRLKDLAELID-NKYEQVDLSKENP 959
Cdd:cd04403      1 FGCHLEALCQRENS-----------TVPKFVRLCIEAVEKRGLDVDGIYRVSGNLAVIQKLRFAVDhDEKLDLDDSKWED 69

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  960 VQ-IAALLKKFLREMPDPLLTFKLHRLFVISQKISDPdkQKRILHLTCCL--LPKAHRDTMEVLFAFLNwtSSFSHVDEe 1036
Cdd:cd04403     70 IHvITGALKLFFRELPEPLFPYSLFNDFVAAIKLSDY--EQRVSAVKDLIksLPKPNHDTLKMLFRHLC--RVIEHGEK- 144

                          170       180
                   ....*....|....*....|....*...
gi 1556664380 1037 tgSKMDIHNLATVITPNILYSNTKNGGM 1064
Cdd:cd04403    145 --NRMTTQNLAIVFGPTLLRPEQETGNI 170

RhoGAP_ARAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in ARAPs. ARAPs (also known as centaurin deltas) contain, besides the RhoGAP domain, an Arf GAP, ankyrin repeat ras-associating, and PH domains. Since their ArfGAP activity is PIP3-dependent, ARAPs are considered integration points for phosphoinositide, Arf and Rho signaling. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  907 IPALVDDAVSAMRQMDMSVEGVFRKNGNIRRLKDLAELI--DNKYEQVDLSKENPVQIAALLKKFLREMPDPLLTFKLHR 984
Cdd:cd04385     15 IPVIVDKCIDFITQHGLMSEGIYRKNGKNSSVKKLLEAFrkDARSVQLREGEYTVHDVADVLKRFLRDLPDPLLTSELHA 94

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  985 LFVISQKISDPD-KQKRILHLTCClLPKAHRDTMEVLFAFLNWTSSFShvDEetgSKMDIHNLATVITPNILYSNTKNGG 1063
Cdd:cd04385     95 EWIEAAELENKDeRIARYKELIRR-LPPINRATLKVLIGHLYRVQKHS--DE---NQMSVHNLALVFGPTLFQTDEHSVG 168

                          170
                   ....*....|....*..
gi 1556664380 1064 MDESflAIEAVTALITY 1080
Cdd:cd04385    169 QTSH--EVKVIEDLIDN 183

RhoGAP_GMIP_PARG1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of GMIP (Gem interacting protein) and PARG1 (PTPL1-associated RhoGAP1). GMIP plays important roles in neurite growth and axonal guidance, and interacts with Gem, a member of the RGK subfamily of the Ras small GTPase superfamily, through the N-terminal half of the protein. GMIP contains a C-terminal RhoGAP domain. GMIP inhibits RhoA function, but is inactive towards Rac1 and Cdc41. PARG1 interacts with Rap2, also a member of the Ras small GTPase superfamily whose exact function is unknown, and shows strong preference for Rho. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  907 IPALVDDAVSAMRQMDMSVEGVFRKNGNIRRLKDLAELIDNKYEQVDLSKENPVQIAALLKKFLREMPDPLLTFKLHRLF 986
Cdd:cd04378     16 VPFIIKKCTSEIENRALGVQGIYRVSGSKARVEKLCQAFENGKDLVELSELSPHDISSVLKLFLRQLPEPLILFRLYNDF 95

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  987 VI----SQKISDPDKQ-------KRILHLT---CCLLPKAHRDTMEVLFAFLNWTSsfshvDEETGSKMDIHNLATVITP 1052
Cdd:cd04378     96 IAlakeIQRDTEEDKApntpievNRIIRKLkdlLRQLPASNYNTLQHLIAHLYRVA-----EQFEENKMSPNNLGIVFGP 170

                   ...
gi 1556664380 1053 NIL 1055
Cdd:cd04378    171 TLI 173

RhoGAP_myosin_IXA: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in myosins IXA. Class IX myosins contain a characteristic head domain, a neck domain and a tail domain which contains a C6H2-zinc binding motif and a Rho-GAP domain. Class IX myosins are single-headed, processive myosins that are partly cytoplasmic, and partly associated with membranes and the actin cytoskeleton. Class IX myosins are implicated in the regulation of neuronal morphogenesis and function of sensory systems, like the inner ear. There are two major isoforms, myosin IXA and IXB with several splice variants, which are both expressed in developing neurons. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  881 FGVALDYLVEKDGAesthgvgpgalrIPALVDDAVSAMRQMDMSVEGVFRKNGNIRRLKDLAELIDNKYEQVDLSKENPV 960
Cdd:cd04406      1 FGVELSRLTSEDRS------------VPLVVEKLINYIEMHGLYTEGIYRKSGSTNKIKELRQGLDTDANSVNLDDYNIH 68

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  961 QIAALLKKFLREMPDPLLTFKLHRLFVISQKISDPDKQKRILHLTCCLLPKAHRDTMEVLFAFLNWTSsfshVDEETgSK 1040
Cdd:cd04406     69 VIASVFKQWLRDLPNPLMTFELYEEFLRAMGLQERRETVRGVYSVIDQLSRTHLNTLERLIFHLVRIA----LQEET-NR 143

                          170
                   ....*....|....*
gi 1556664380 1041 MDIHNLATVITPNIL 1055
Cdd:cd04406    144 MSANALAIVFAPCIL 158

The third LIM domain of Lrg1p, a LIM and RhoGap domain containing protein; The third LIM domain of Lrg1p, a LIM and RhoGap domain containing protein: The members of this family contain three tandem repeats of LIM domains and a Rho-type GTPase activating protein (RhoGap) domain. Lrg1p is a Rho1 GTPase-activating protein required for efficient cell fusion in yeast. Lrg1p-GAP domain strongly and specifically stimulates the GTPase activity of Rho1p, a regulator of beta (1-3)-glucan synthase in vitro. The LIM domain is 50-60 amino acids in size and shares two characteristic zinc finger motifs. The two zinc fingers contain eight conserved residues, mostly cysteines and histidines, which coordinately bond to two zinc atoms. LIM domains function as adaptors or scaffolds to support the assembly of multimeric protein.

                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*...
gi 1556664380  515 CTACREPIDDECIILGERRWHLKPphLQCAACQKDLTVDLADGKWNETNNRVFCSSCA 572
Cdd:cd09393      1 CASCGKSIEDECIKFEDKRWHLKC--FTCSRCHREISSELSDAAFNNKDQRILCSNCS 56

RhoGAP_KIAA1688: GTPase-activator protein (GAP) domain for Rho-like GTPases found in KIAA1688-like proteins; KIAA1688 is a protein of unknown function that contains a RhoGAP domain and a myosin tail homology 4 (MyTH4) domain. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1556664380  926 EGVFRKNGNIRRLKDLAELIDN-KYEQVDLskENPVQIAALLKKFLREMPDPLLTFKLHRLFVISQKisDPDKQKRILHL 1004
Cdd:cd04389     41 EGIFRVPGDIDEVNELKLRVDQwDYPLSGL--EDPHVPASLLKLWLRELEEPLIPDALYQQCISASE--DPDKAVEIVQK 116

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|.
gi 1556664380 1005 tcclLPKAHRDTMEVLFAFLNWTSSFSHVDEetgSKMDIHNLATVITPNIL 1055
Cdd:cd04389    117 ----LPIINRLVLCYLINFLQVFAQPENVAH---TKMDVSNLAMVFAPNIL 160